Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074616.t1 | XP_013468051.1 | 98.8 | 165 | 2 | 0 | 47 | 211 | 347 | 511 | 1.00E-88 | 336.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074616.t1 | Q9LZS0 | 40.8 | 120 | 66 | 2 | 1 | 115 | 302 | 421 | 3.0e-15 | 83.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074616.t1 | A0A072VKX9 | 98.8 | 165 | 2 | 0 | 47 | 211 | 347 | 511 | 7.6e-89 | 336.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene074616 | 0.826321 | 2.89E-54 | -1.69E-46 |
| MS.gene049255 | MS.gene074616 | 0.807058 | 6.09E-50 | -1.69E-46 |
| MS.gene049341 | MS.gene074616 | 0.830952 | 2.20E-55 | -1.69E-46 |
| MS.gene049501 | MS.gene074616 | 0.802289 | 6.02E-49 | -1.69E-46 |
| MS.gene049821 | MS.gene074616 | 0.804605 | 1.99E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene074616 | 0.867098 | 1.76E-65 | -1.69E-46 |
| MS.gene050303 | MS.gene074616 | 0.827108 | 1.88E-54 | -1.69E-46 |
| MS.gene050438 | MS.gene074616 | 0.813604 | 2.35E-51 | -1.69E-46 |
| MS.gene050586 | MS.gene074616 | 0.801164 | 1.02E-48 | -1.69E-46 |
| MS.gene050807 | MS.gene074616 | 0.853729 | 1.97E-61 | -1.69E-46 |
| MS.gene050808 | MS.gene074616 | 0.827905 | 1.21E-54 | -1.69E-46 |
| MS.gene050815 | MS.gene074616 | 0.873842 | 1.07E-67 | -1.69E-46 |
| MS.gene051180 | MS.gene074616 | 0.813231 | 2.84E-51 | -1.69E-46 |
| MS.gene051243 | MS.gene074616 | 0.810985 | 8.78E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene074616 | 0.803945 | 2.74E-49 | -1.69E-46 |
| MS.gene051328 | MS.gene074616 | 0.838215 | 3.28E-57 | -1.69E-46 |
| MS.gene051388 | MS.gene074616 | 0.806596 | 7.62E-50 | -1.69E-46 |
| MS.gene05157 | MS.gene074616 | 0.801436 | 9.01E-49 | -1.69E-46 |
| MS.gene051626 | MS.gene074616 | 0.818312 | 2.09E-52 | -1.69E-46 |
| MS.gene051719 | MS.gene074616 | 0.827196 | 1.79E-54 | -1.69E-46 |
| MS.gene051720 | MS.gene074616 | 0.819939 | 8.93E-53 | -1.69E-46 |
| MS.gene051789 | MS.gene074616 | 0.809644 | 1.71E-50 | -1.69E-46 |
| MS.gene052012 | MS.gene074616 | 0.806137 | 9.52E-50 | -1.69E-46 |
| MS.gene052020 | MS.gene074616 | 0.802148 | 6.44E-49 | -1.69E-46 |
| MS.gene052133 | MS.gene074616 | 0.804609 | 1.99E-49 | -1.69E-46 |
| MS.gene052613 | MS.gene074616 | 0.802636 | 5.11E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene074616 | 0.83302 | 6.78E-56 | -1.69E-46 |
| MS.gene052863 | MS.gene074616 | 0.825652 | 4.17E-54 | -1.69E-46 |
| MS.gene052896 | MS.gene074616 | 0.809054 | 2.29E-50 | -1.69E-46 |
| MS.gene053008 | MS.gene074616 | 0.841496 | 4.59E-58 | -1.69E-46 |
| MS.gene053074 | MS.gene074616 | 0.841463 | 4.68E-58 | -1.69E-46 |
| MS.gene053221 | MS.gene074616 | 0.806535 | 7.85E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene074616 | 0.812434 | 4.25E-51 | -1.69E-46 |
| MS.gene053630 | MS.gene074616 | 0.820962 | 5.20E-53 | -1.69E-46 |
| MS.gene054185 | MS.gene074616 | 0.804297 | 2.31E-49 | -1.69E-46 |
| MS.gene054472 | MS.gene074616 | 0.828361 | 9.38E-55 | -1.69E-46 |
| MS.gene055358 | MS.gene074616 | 0.800186 | 1.62E-48 | -1.69E-46 |
| MS.gene055465 | MS.gene074616 | 0.820918 | 5.32E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene074616 | 0.849544 | 3.02E-60 | -1.69E-46 |
| MS.gene055468 | MS.gene074616 | 0.842965 | 1.87E-58 | -1.69E-46 |
| MS.gene055512 | MS.gene074616 | 0.814266 | 1.68E-51 | -1.69E-46 |
| MS.gene055608 | MS.gene074616 | 0.847598 | 1.04E-59 | -1.69E-46 |
| MS.gene055818 | MS.gene074616 | 0.823346 | 1.46E-53 | -1.69E-46 |
| MS.gene056069 | MS.gene074616 | -0.806951 | 6.41E-50 | -1.69E-46 |
| MS.gene056215 | MS.gene074616 | 0.805743 | 1.15E-49 | -1.69E-46 |
| MS.gene056760 | MS.gene074616 | 0.815117 | 1.09E-51 | -1.69E-46 |
| MS.gene057057 | MS.gene074616 | 0.807932 | 3.97E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene074616 | 0.833484 | 5.19E-56 | -1.69E-46 |
| MS.gene057260 | MS.gene074616 | 0.800371 | 1.49E-48 | -1.69E-46 |
| MS.gene057693 | MS.gene074616 | 0.836306 | 1.01E-56 | -1.69E-46 |
| MS.gene058084 | MS.gene074616 | 0.801871 | 7.34E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene074616 | 0.83323 | 6.01E-56 | -1.69E-46 |
| MS.gene058906 | MS.gene074616 | 0.805372 | 1.38E-49 | -1.69E-46 |
| MS.gene059006 | MS.gene074616 | 0.827887 | 1.22E-54 | -1.69E-46 |
| MS.gene059151 | MS.gene074616 | 0.811344 | 7.34E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene074616 | 0.805985 | 1.02E-49 | -1.69E-46 |
| MS.gene059607 | MS.gene074616 | 0.800019 | 1.75E-48 | -1.69E-46 |
| MS.gene059842 | MS.gene074616 | 0.827 | 1.99E-54 | -1.69E-46 |
| MS.gene059937 | MS.gene074616 | 0.827476 | 1.53E-54 | -1.69E-46 |
| MS.gene060137 | MS.gene074616 | -0.814379 | 1.59E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene074616 | 0.818819 | 1.61E-52 | -1.69E-46 |
| MS.gene060429 | MS.gene074616 | 0.834193 | 3.45E-56 | -1.69E-46 |
| MS.gene060587 | MS.gene074616 | 0.807014 | 6.22E-50 | -1.69E-46 |
| MS.gene060802 | MS.gene074616 | 0.807593 | 4.69E-50 | -1.69E-46 |
| MS.gene060803 | MS.gene074616 | 0.855618 | 5.59E-62 | -1.69E-46 |
| MS.gene061176 | MS.gene074616 | 0.803487 | 3.41E-49 | -1.69E-46 |
| MS.gene061177 | MS.gene074616 | 0.80324 | 3.83E-49 | -1.69E-46 |
| MS.gene061178 | MS.gene074616 | 0.803039 | 4.22E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074616.t1 | MTR_1g060990 | 99.052 | 211 | 2 | 0 | 1 | 211 | 301 | 511 | 5.53e-155 | 439 |
| MS.gene074616.t1 | MTR_1g098900 | 40.000 | 70 | 42 | 0 | 1 | 70 | 288 | 357 | 5.27e-12 | 64.7 |
| MS.gene074616.t1 | MTR_1g492790 | 36.000 | 75 | 47 | 1 | 1 | 74 | 311 | 385 | 6.99e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074616.t1 | AT3G10000 | 36.771 | 223 | 116 | 7 | 1 | 211 | 261 | 470 | 4.17e-30 | 115 |
| MS.gene074616.t1 | AT3G10000 | 36.771 | 223 | 116 | 7 | 1 | 211 | 271 | 480 | 4.93e-30 | 115 |
| MS.gene074616.t1 | AT5G03680 | 45.000 | 120 | 61 | 2 | 1 | 115 | 302 | 421 | 8.46e-25 | 101 |
| MS.gene074616.t1 | AT1G76880 | 42.857 | 70 | 40 | 0 | 1 | 70 | 262 | 331 | 8.63e-11 | 61.2 |
Find 0 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCAAAATCAAAATGAAGA+TGG | - | 6961:4827-4846 | MS.gene074616:CDS | 20.0% |
| !! | TTCAAAATCAAAATGAAGAT+GGG | - | 6961:4828-4847 | MS.gene074616:CDS | 20.0% |
| !! | ATGATTCTAAAGAAGGAAAT+AGG | - | 6961:4903-4922 | MS.gene074616:CDS | 25.0% |
| !! | TGGAAAAAGAACAAAAGTTT+TGG | - | 6961:4669-4688 | MS.gene074616:CDS | 25.0% |
| AAACTACAAAGGAATGATCA+TGG | - | 6961:5028-5047 | MS.gene074616:CDS | 30.0% | |
| AATGTTGGAAATGTTGCTAA+TGG | - | 6961:5070-5089 | MS.gene074616:CDS | 30.0% | |
| AGAAATGATTAAGGTTGAAG+AGG | - | 6961:4763-4782 | MS.gene074616:CDS | 30.0% | |
| ATGAGATTGTGAAACTACAA+AGG | - | 6961:5017-5036 | MS.gene074616:CDS | 30.0% | |
| TTCTGATAATTGTACTCCAA+GGG | - | 6961:4880-4899 | MS.gene074616:CDS | 30.0% | |
| TTTCCAACATTAGAATCAGA+AGG | + | 6961:5061-5080 | MS.gene074616:intergenic | 30.0% | |
| TTTCCTTTCATGATTAGTGA+GGG | - | 6961:5103-5122 | MS.gene074616:CDS | 30.0% | |
| ! | AAGGGTGTAAATGTAATAAC+TGG | - | 6961:4797-4816 | MS.gene074616:CDS | 30.0% |
| !! | ATGGAAGCTTTGAAGAATTT+AGG | - | 6961:4734-4753 | MS.gene074616:CDS | 30.0% |
| AGAAGGAAATAGGAAGAGAA+AGG | - | 6961:4913-4932 | MS.gene074616:CDS | 35.0% | |
| AGGAGGTAGAGAAATGATTA+AGG | - | 6961:4754-4773 | MS.gene074616:CDS | 35.0% | |
| ATCTTGAATAGTACTGTGAG+AGG | - | 6961:4857-4876 | MS.gene074616:CDS | 35.0% | |
| CAATGCTCTATGTACAATCA+AGG | - | 6961:4962-4981 | MS.gene074616:CDS | 35.0% | |
| CTTTCCTTTCATGATTAGTG+AGG | - | 6961:5102-5121 | MS.gene074616:CDS | 35.0% | |
| GTTCTGATAATTGTACTCCA+AGG | - | 6961:4879-4898 | MS.gene074616:CDS | 35.0% | |
| TCACCTTCTGATTCTAATGT+TGG | - | 6961:5055-5074 | MS.gene074616:CDS | 35.0% | |
| TCTATGTACAATCAAGGAAG+TGG | - | 6961:4968-4987 | MS.gene074616:CDS | 35.0% | |
| ! | GGAAATTTGTGGGAGAATTT+TGG | - | 6961:5127-5146 | MS.gene074616:CDS | 35.0% |
| ! | TTGTTCTTTTTCCACCCTTT+TGG | + | 6961:4663-4682 | MS.gene074616:intergenic | 35.0% |
| !! | GAAGCTTTGAAGAATTTAGG+AGG | - | 6961:4737-4756 | MS.gene074616:CDS | 35.0% |
| GAAGAGGCTCATGATCATAA+GGG | - | 6961:4779-4798 | MS.gene074616:CDS | 40.0% | |
| TAAGCTTGTGAAGACACTTG+AGG | - | 6961:4586-4605 | MS.gene074616:CDS | 40.0% | |
| TAGTGAGGGTGGAAATTTGT+GGG | - | 6961:5117-5136 | MS.gene074616:CDS | 40.0% | |
| TGAAGAGGCTCATGATCATA+AGG | - | 6961:4778-4797 | MS.gene074616:CDS | 40.0% | |
| TTAGTGAGGGTGGAAATTTG+TGG | - | 6961:5116-5135 | MS.gene074616:CDS | 40.0% | |
| ! | CCAAGGGATGATTCTAAAGA+AGG | - | 6961:4896-4915 | MS.gene074616:CDS | 40.0% |
| ! | TTTGGAGTAATGAGAGAGCA+TGG | - | 6961:4687-4706 | MS.gene074616:CDS | 40.0% |
| !! | CCTTCTTTAGAATCATCCCT+TGG | + | 6961:4899-4918 | MS.gene074616:intergenic | 40.0% |
| AGAAGAGTGGAGGAAACAAG+AGG | - | 6961:4637-4656 | MS.gene074616:CDS | 45.0% | |
| CCACCCTCACTAATCATGAA+AGG | + | 6961:5109-5128 | MS.gene074616:intergenic | 45.0% | |
| CCTTTCATGATTAGTGAGGG+TGG | - | 6961:5106-5125 | MS.gene074616:CDS | 45.0% | |
| CGTTAAGAGAAGAAGAGTGG+AGG | - | 6961:4627-4646 | MS.gene074616:CDS | 45.0% | |
| GACACTTGAGGACAAGGAAA+AGG | - | 6961:4598-4617 | MS.gene074616:CDS | 45.0% | |
| GAGGAAACAAGAGGCCAAAA+GGG | - | 6961:4646-4665 | MS.gene074616:CDS | 45.0% | |
| TGAGGACAAGGAAAAGGAGA+GGG | - | 6961:4604-4623 | MS.gene074616:CDS | 45.0% | |
| TGTGAAGACACTTGAGGACA+AGG | - | 6961:4592-4611 | MS.gene074616:CDS | 45.0% | |
| TTGAGGACAAGGAAAAGGAG+AGG | - | 6961:4603-4622 | MS.gene074616:CDS | 45.0% | |
| ! | AGAGAGCATGGATTGAAGCA+AGG | - | 6961:4699-4718 | MS.gene074616:CDS | 45.0% |
| ! | GAGAGCATGGATTGAAGCAA+GGG | - | 6961:4700-4719 | MS.gene074616:CDS | 45.0% |
| !! | AGCAAGGGATGCTGCTTTAA+TGG | - | 6961:4715-4734 | MS.gene074616:CDS | 45.0% |
| GAAACAAGAGGCCAAAAGGG+TGG | - | 6961:4649-4668 | MS.gene074616:CDS | 50.0% | |
| GAAGCTTGTGGAGAAGCAAG+AGG | - | 6961:4553-4572 | MS.gene074616:CDS | 50.0% | |
| GGAGGAAACAAGAGGCCAAA+AGG | - | 6961:4645-4664 | MS.gene074616:CDS | 50.0% | |
| GGGCGTTAAGAGAAGAAGAG+TGG | - | 6961:4624-4643 | MS.gene074616:CDS | 50.0% | |
| TTGTGGAGAAGCAAGAGGAG+TGG | - | 6961:4558-4577 | MS.gene074616:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 6961 | gene | 4548 | 5183 | 4548 | ID=MS.gene074616 |
| 6961 | mRNA | 4548 | 5183 | 4548 | ID=MS.gene074616.t1;Parent=MS.gene074616 |
| 6961 | exon | 4548 | 5183 | 4548 | ID=MS.gene074616.t1.exon1;Parent=MS.gene074616.t1 |
| 6961 | CDS | 4548 | 5183 | 4548 | ID=cds.MS.gene074616.t1;Parent=MS.gene074616.t1 |
| Gene Sequence |
| Protein sequence |
