Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074617.t1 | XP_013468051.1 | 98.8 | 165 | 2 | 0 | 47 | 211 | 347 | 511 | 1.00E-88 | 336.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074617.t1 | Q9LZS0 | 28.7 | 293 | 115 | 9 | 1 | 206 | 302 | 587 | 1.2e-16 | 88.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074617.t1 | A0A072VKX9 | 98.8 | 165 | 2 | 0 | 47 | 211 | 347 | 511 | 7.6e-89 | 336.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene074617 | 0.826801 | 2.22E-54 | -1.69E-46 |
| MS.gene049255 | MS.gene074617 | 0.808131 | 3.60E-50 | -1.69E-46 |
| MS.gene049341 | MS.gene074617 | 0.832308 | 1.02E-55 | -1.69E-46 |
| MS.gene049501 | MS.gene074617 | 0.802688 | 4.98E-49 | -1.69E-46 |
| MS.gene049821 | MS.gene074617 | 0.804799 | 1.82E-49 | -1.69E-46 |
| MS.gene050020 | MS.gene074617 | 0.865427 | 5.95E-65 | -1.69E-46 |
| MS.gene050303 | MS.gene074617 | 0.829582 | 4.75E-55 | -1.69E-46 |
| MS.gene050438 | MS.gene074617 | 0.815305 | 9.90E-52 | -1.69E-46 |
| MS.gene050586 | MS.gene074617 | 0.80006 | 1.72E-48 | -1.69E-46 |
| MS.gene050807 | MS.gene074617 | 0.853323 | 2.58E-61 | -1.69E-46 |
| MS.gene050808 | MS.gene074617 | 0.827154 | 1.83E-54 | -1.69E-46 |
| MS.gene050815 | MS.gene074617 | 0.875053 | 4.17E-68 | -1.69E-46 |
| MS.gene051180 | MS.gene074617 | 0.813838 | 2.09E-51 | -1.69E-46 |
| MS.gene051243 | MS.gene074617 | 0.81272 | 3.68E-51 | -1.69E-46 |
| MS.gene051247 | MS.gene074617 | 0.803732 | 3.03E-49 | -1.69E-46 |
| MS.gene051328 | MS.gene074617 | 0.838936 | 2.14E-57 | -1.69E-46 |
| MS.gene051344 | MS.gene074617 | 0.800869 | 1.18E-48 | -1.69E-46 |
| MS.gene05134 | MS.gene074617 | 0.8007 | 1.27E-48 | -1.69E-46 |
| MS.gene051388 | MS.gene074617 | 0.805097 | 1.57E-49 | -1.69E-46 |
| MS.gene051626 | MS.gene074617 | 0.819461 | 1.15E-52 | -1.69E-46 |
| MS.gene051719 | MS.gene074617 | 0.825674 | 4.12E-54 | -1.69E-46 |
| MS.gene051720 | MS.gene074617 | 0.817449 | 3.28E-52 | -1.69E-46 |
| MS.gene051789 | MS.gene074617 | 0.810046 | 1.40E-50 | -1.69E-46 |
| MS.gene052012 | MS.gene074617 | 0.80719 | 5.71E-50 | -1.69E-46 |
| MS.gene052020 | MS.gene074617 | 0.802424 | 5.65E-49 | -1.69E-46 |
| MS.gene052133 | MS.gene074617 | 0.805067 | 1.60E-49 | -1.69E-46 |
| MS.gene052613 | MS.gene074617 | 0.802816 | 4.69E-49 | -1.69E-46 |
| MS.gene052665 | MS.gene074617 | 0.833015 | 6.80E-56 | -1.69E-46 |
| MS.gene052863 | MS.gene074617 | 0.824197 | 9.20E-54 | -1.69E-46 |
| MS.gene052896 | MS.gene074617 | 0.809497 | 1.84E-50 | -1.69E-46 |
| MS.gene053008 | MS.gene074617 | 0.841643 | 4.20E-58 | -1.69E-46 |
| MS.gene053074 | MS.gene074617 | 0.842545 | 2.42E-58 | -1.69E-46 |
| MS.gene053221 | MS.gene074617 | 0.805167 | 1.52E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene074617 | 0.810835 | 9.46E-51 | -1.69E-46 |
| MS.gene053630 | MS.gene074617 | 0.823022 | 1.73E-53 | -1.69E-46 |
| MS.gene054184 | MS.gene074617 | 0.801684 | 8.02E-49 | -1.69E-46 |
| MS.gene054185 | MS.gene074617 | 0.806977 | 6.33E-50 | -1.69E-46 |
| MS.gene054472 | MS.gene074617 | 0.827149 | 1.83E-54 | -1.69E-46 |
| MS.gene055465 | MS.gene074617 | 0.819078 | 1.40E-52 | -1.69E-46 |
| MS.gene055466 | MS.gene074617 | 0.848435 | 6.14E-60 | -1.69E-46 |
| MS.gene055468 | MS.gene074617 | 0.842344 | 2.74E-58 | -1.69E-46 |
| MS.gene055512 | MS.gene074617 | 0.813525 | 2.45E-51 | -1.69E-46 |
| MS.gene055608 | MS.gene074617 | 0.847632 | 1.02E-59 | -1.69E-46 |
| MS.gene055818 | MS.gene074617 | 0.821501 | 3.91E-53 | -1.69E-46 |
| MS.gene056069 | MS.gene074617 | -0.80799 | 3.86E-50 | -1.69E-46 |
| MS.gene056215 | MS.gene074617 | 0.806817 | 6.84E-50 | -1.69E-46 |
| MS.gene056760 | MS.gene074617 | 0.814552 | 1.45E-51 | -1.69E-46 |
| MS.gene057057 | MS.gene074617 | 0.810558 | 1.09E-50 | -1.69E-46 |
| MS.gene057060 | MS.gene074617 | 0.832927 | 7.15E-56 | -1.69E-46 |
| MS.gene057260 | MS.gene074617 | 0.803169 | 3.96E-49 | -1.69E-46 |
| MS.gene057693 | MS.gene074617 | 0.836772 | 7.69E-57 | -1.69E-46 |
| MS.gene058084 | MS.gene074617 | 0.80448 | 2.12E-49 | -1.69E-46 |
| MS.gene058168 | MS.gene074617 | 0.833701 | 4.58E-56 | -1.69E-46 |
| MS.gene058906 | MS.gene074617 | 0.80842 | 3.12E-50 | -1.69E-46 |
| MS.gene059006 | MS.gene074617 | 0.828749 | 7.56E-55 | -1.69E-46 |
| MS.gene059151 | MS.gene074617 | 0.812236 | 4.69E-51 | -1.69E-46 |
| MS.gene05954 | MS.gene074617 | 0.806062 | 9.87E-50 | -1.69E-46 |
| MS.gene059842 | MS.gene074617 | 0.826997 | 2.00E-54 | -1.69E-46 |
| MS.gene059937 | MS.gene074617 | 0.827491 | 1.52E-54 | -1.69E-46 |
| MS.gene060137 | MS.gene074617 | -0.81379 | 2.14E-51 | -1.69E-46 |
| MS.gene060322 | MS.gene074617 | 0.818391 | 2.01E-52 | -1.69E-46 |
| MS.gene060429 | MS.gene074617 | 0.834874 | 2.33E-56 | -1.69E-46 |
| MS.gene060587 | MS.gene074617 | 0.806897 | 6.58E-50 | -1.69E-46 |
| MS.gene060802 | MS.gene074617 | 0.808171 | 3.53E-50 | -1.69E-46 |
| MS.gene060803 | MS.gene074617 | 0.856107 | 4.02E-62 | -1.69E-46 |
| MS.gene061176 | MS.gene074617 | 0.800555 | 1.36E-48 | -1.69E-46 |
| MS.gene061177 | MS.gene074617 | 0.800325 | 1.52E-48 | -1.69E-46 |
| MS.gene061178 | MS.gene074617 | 0.800117 | 1.68E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074617.t1 | MTR_1g060990 | 99.052 | 211 | 2 | 0 | 1 | 211 | 301 | 511 | 5.53e-155 | 439 |
| MS.gene074617.t1 | MTR_1g098900 | 40.000 | 70 | 42 | 0 | 1 | 70 | 288 | 357 | 5.27e-12 | 64.7 |
| MS.gene074617.t1 | MTR_1g492790 | 36.000 | 75 | 47 | 1 | 1 | 74 | 311 | 385 | 6.99e-12 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074617.t1 | AT3G10000 | 36.771 | 223 | 116 | 7 | 1 | 211 | 261 | 470 | 4.17e-30 | 115 |
| MS.gene074617.t1 | AT3G10000 | 36.771 | 223 | 116 | 7 | 1 | 211 | 271 | 480 | 4.93e-30 | 115 |
| MS.gene074617.t1 | AT5G03680 | 45.000 | 120 | 61 | 2 | 1 | 115 | 302 | 421 | 8.46e-25 | 101 |
| MS.gene074617.t1 | AT1G76880 | 42.857 | 70 | 40 | 0 | 1 | 70 | 262 | 331 | 8.63e-11 | 61.2 |
Find 0 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTCAAAATCAAAATGAAGA+TGG | - | 6962:4397-4416 | MS.gene074617:CDS | 20.0% |
| !! | TTCAAAATCAAAATGAAGAT+GGG | - | 6962:4398-4417 | MS.gene074617:CDS | 20.0% |
| !! | ATGATTCTAAAGAAGGAAAT+AGG | - | 6962:4473-4492 | MS.gene074617:CDS | 25.0% |
| !! | TGGAAAAAGAACAAAAGTTT+TGG | - | 6962:4239-4258 | MS.gene074617:CDS | 25.0% |
| AAACTACAAAGGAATGATCA+TGG | - | 6962:4598-4617 | MS.gene074617:CDS | 30.0% | |
| AATGTTGGAAATGTTGCTAA+TGG | - | 6962:4640-4659 | MS.gene074617:CDS | 30.0% | |
| AGAAATGATTAAGGTTGAAG+AGG | - | 6962:4333-4352 | MS.gene074617:CDS | 30.0% | |
| ATGAGATTGTGAAACTACAA+AGG | - | 6962:4587-4606 | MS.gene074617:CDS | 30.0% | |
| TTCTGATAATTGTACTCCAA+GGG | - | 6962:4450-4469 | MS.gene074617:CDS | 30.0% | |
| TTTCCAACATTAGAATCAGA+AGG | + | 6962:4631-4650 | MS.gene074617:intergenic | 30.0% | |
| TTTCCTTTCATGATTAGTGA+GGG | - | 6962:4673-4692 | MS.gene074617:CDS | 30.0% | |
| ! | AAGGGTGTAAATGTAATAAC+TGG | - | 6962:4367-4386 | MS.gene074617:CDS | 30.0% |
| !! | ATGGAAGCTTTGAAGAATTT+AGG | - | 6962:4304-4323 | MS.gene074617:CDS | 30.0% |
| AGAAGGAAATAGGAAGAGAA+AGG | - | 6962:4483-4502 | MS.gene074617:CDS | 35.0% | |
| AGGAGGTAGAGAAATGATTA+AGG | - | 6962:4324-4343 | MS.gene074617:CDS | 35.0% | |
| ATCTTGAATAGTACTGTGAG+AGG | - | 6962:4427-4446 | MS.gene074617:CDS | 35.0% | |
| CAATGCTCTATGTACAATCA+AGG | - | 6962:4532-4551 | MS.gene074617:CDS | 35.0% | |
| CTTTCCTTTCATGATTAGTG+AGG | - | 6962:4672-4691 | MS.gene074617:CDS | 35.0% | |
| GTTCTGATAATTGTACTCCA+AGG | - | 6962:4449-4468 | MS.gene074617:CDS | 35.0% | |
| TCACCTTCTGATTCTAATGT+TGG | - | 6962:4625-4644 | MS.gene074617:CDS | 35.0% | |
| TCTATGTACAATCAAGGAAG+TGG | - | 6962:4538-4557 | MS.gene074617:CDS | 35.0% | |
| ! | GGAAATTTGTGGGAGAATTT+TGG | - | 6962:4697-4716 | MS.gene074617:CDS | 35.0% |
| ! | TTGTTCTTTTTCCACCCTTT+TGG | + | 6962:4233-4252 | MS.gene074617:intergenic | 35.0% |
| !! | GAAGCTTTGAAGAATTTAGG+AGG | - | 6962:4307-4326 | MS.gene074617:CDS | 35.0% |
| GAAGAGGCTCATGATCATAA+GGG | - | 6962:4349-4368 | MS.gene074617:CDS | 40.0% | |
| TAAGCTTGTGAAGACACTTG+AGG | - | 6962:4156-4175 | MS.gene074617:CDS | 40.0% | |
| TAGTGAGGGTGGAAATTTGT+GGG | - | 6962:4687-4706 | MS.gene074617:CDS | 40.0% | |
| TGAAGAGGCTCATGATCATA+AGG | - | 6962:4348-4367 | MS.gene074617:CDS | 40.0% | |
| TTAGTGAGGGTGGAAATTTG+TGG | - | 6962:4686-4705 | MS.gene074617:CDS | 40.0% | |
| ! | CCAAGGGATGATTCTAAAGA+AGG | - | 6962:4466-4485 | MS.gene074617:CDS | 40.0% |
| ! | TTTGGAGTAATGAGAGAGCA+TGG | - | 6962:4257-4276 | MS.gene074617:CDS | 40.0% |
| !! | CCTTCTTTAGAATCATCCCT+TGG | + | 6962:4469-4488 | MS.gene074617:intergenic | 40.0% |
| AGAAGAGTGGAGGAAACAAG+AGG | - | 6962:4207-4226 | MS.gene074617:CDS | 45.0% | |
| CCACCCTCACTAATCATGAA+AGG | + | 6962:4679-4698 | MS.gene074617:intergenic | 45.0% | |
| CCTTTCATGATTAGTGAGGG+TGG | - | 6962:4676-4695 | MS.gene074617:CDS | 45.0% | |
| CGTTAAGAGAAGAAGAGTGG+AGG | - | 6962:4197-4216 | MS.gene074617:CDS | 45.0% | |
| GACACTTGAGGACAAGGAAA+AGG | - | 6962:4168-4187 | MS.gene074617:CDS | 45.0% | |
| GAGGAAACAAGAGGCCAAAA+GGG | - | 6962:4216-4235 | MS.gene074617:CDS | 45.0% | |
| TGAGGACAAGGAAAAGGAGA+GGG | - | 6962:4174-4193 | MS.gene074617:CDS | 45.0% | |
| TGTGAAGACACTTGAGGACA+AGG | - | 6962:4162-4181 | MS.gene074617:CDS | 45.0% | |
| TTGAGGACAAGGAAAAGGAG+AGG | - | 6962:4173-4192 | MS.gene074617:CDS | 45.0% | |
| ! | AGAGAGCATGGATTGAAGCA+AGG | - | 6962:4269-4288 | MS.gene074617:CDS | 45.0% |
| ! | GAGAGCATGGATTGAAGCAA+GGG | - | 6962:4270-4289 | MS.gene074617:CDS | 45.0% |
| !! | AGCAAGGGATGCTGCTTTAA+TGG | - | 6962:4285-4304 | MS.gene074617:CDS | 45.0% |
| GAAACAAGAGGCCAAAAGGG+TGG | - | 6962:4219-4238 | MS.gene074617:CDS | 50.0% | |
| GAAGCTTGTGGAGAAGCAAG+AGG | - | 6962:4123-4142 | MS.gene074617:CDS | 50.0% | |
| GGAGGAAACAAGAGGCCAAA+AGG | - | 6962:4215-4234 | MS.gene074617:CDS | 50.0% | |
| GGGCGTTAAGAGAAGAAGAG+TGG | - | 6962:4194-4213 | MS.gene074617:CDS | 50.0% | |
| TTGTGGAGAAGCAAGAGGAG+TGG | - | 6962:4128-4147 | MS.gene074617:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 6962 | gene | 4118 | 4753 | 4118 | ID=MS.gene074617 |
| 6962 | mRNA | 4118 | 4753 | 4118 | ID=MS.gene074617.t1;Parent=MS.gene074617 |
| 6962 | exon | 4118 | 4753 | 4118 | ID=MS.gene074617.t1.exon1;Parent=MS.gene074617.t1 |
| 6962 | CDS | 4118 | 4753 | 4118 | ID=cds.MS.gene074617.t1;Parent=MS.gene074617.t1 |
| Gene Sequence |
| Protein sequence |
