Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08616.t1 | XP_003606407.1 | 93.1 | 218 | 15 | 0 | 1 | 218 | 1 | 218 | 2.50E-117 | 431.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08616.t1 | Q9SR36 | 45.6 | 215 | 113 | 3 | 3 | 215 | 5 | 217 | 6.1e-48 | 192.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08616.t1 | G7JPE9 | 93.1 | 218 | 15 | 0 | 1 | 218 | 1 | 218 | 1.8e-117 | 431.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04932 | MS.gene08616 | 0.800117 | 1.68E-48 | -1.69E-46 |
| MS.gene049611 | MS.gene08616 | 0.822122 | 2.80E-53 | -1.69E-46 |
| MS.gene049634 | MS.gene08616 | 0.813565 | 2.40E-51 | -1.69E-46 |
| MS.gene050082 | MS.gene08616 | 0.827352 | 1.64E-54 | -1.69E-46 |
| MS.gene050323 | MS.gene08616 | -0.828676 | 7.87E-55 | -1.69E-46 |
| MS.gene050381 | MS.gene08616 | 0.823041 | 1.71E-53 | -1.69E-46 |
| MS.gene050496 | MS.gene08616 | -0.804249 | 2.36E-49 | -1.69E-46 |
| MS.gene050612 | MS.gene08616 | -0.811406 | 7.11E-51 | -1.69E-46 |
| MS.gene050675 | MS.gene08616 | -0.801228 | 9.94E-49 | -1.69E-46 |
| MS.gene05079 | MS.gene08616 | 0.80292 | 4.46E-49 | -1.69E-46 |
| MS.gene050809 | MS.gene08616 | 0.84044 | 8.68E-58 | -1.69E-46 |
| MS.gene050833 | MS.gene08616 | 0.808385 | 3.18E-50 | -1.69E-46 |
| MS.gene050868 | MS.gene08616 | -0.807888 | 4.06E-50 | -1.69E-46 |
| MS.gene050871 | MS.gene08616 | -0.807925 | 3.98E-50 | -1.69E-46 |
| MS.gene05104 | MS.gene08616 | 0.821793 | 3.34E-53 | -1.69E-46 |
| MS.gene051127 | MS.gene08616 | 0.832068 | 1.17E-55 | -1.69E-46 |
| MS.gene051138 | MS.gene08616 | 0.844049 | 9.62E-59 | -1.69E-46 |
| MS.gene051364 | MS.gene08616 | 0.811875 | 5.62E-51 | -1.69E-46 |
| MS.gene051366 | MS.gene08616 | 0.808967 | 2.39E-50 | -1.69E-46 |
| MS.gene051422 | MS.gene08616 | 0.828159 | 1.05E-54 | -1.69E-46 |
| MS.gene051632 | MS.gene08616 | 0.840733 | 7.28E-58 | -1.69E-46 |
| MS.gene051651 | MS.gene08616 | 0.821008 | 5.07E-53 | -1.69E-46 |
| MS.gene051863 | MS.gene08616 | 0.809409 | 1.92E-50 | -1.69E-46 |
| MS.gene052129 | MS.gene08616 | 0.803604 | 3.22E-49 | -1.69E-46 |
| MS.gene05240 | MS.gene08616 | 0.805864 | 1.09E-49 | -1.69E-46 |
| MS.gene053420 | MS.gene08616 | 0.842353 | 2.72E-58 | -1.69E-46 |
| MS.gene053479 | MS.gene08616 | -0.803194 | 3.92E-49 | -1.69E-46 |
| MS.gene054706 | MS.gene08616 | 0.828865 | 7.09E-55 | -1.69E-46 |
| MS.gene055154 | MS.gene08616 | 0.821826 | 3.28E-53 | -1.69E-46 |
| MS.gene055158 | MS.gene08616 | 0.816035 | 6.81E-52 | -1.69E-46 |
| MS.gene055202 | MS.gene08616 | 0.824056 | 9.92E-54 | -1.69E-46 |
| MS.gene055502 | MS.gene08616 | -0.807183 | 5.72E-50 | -1.69E-46 |
| MS.gene055577 | MS.gene08616 | -0.811547 | 6.63E-51 | -1.69E-46 |
| MS.gene05589 | MS.gene08616 | -0.806396 | 8.39E-50 | -1.69E-46 |
| MS.gene055959 | MS.gene08616 | 0.816268 | 6.04E-52 | -1.69E-46 |
| MS.gene056224 | MS.gene08616 | 0.822125 | 2.80E-53 | -1.69E-46 |
| MS.gene056387 | MS.gene08616 | -0.808197 | 3.49E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene08616 | -0.821212 | 4.55E-53 | -1.69E-46 |
| MS.gene056590 | MS.gene08616 | 0.813528 | 2.45E-51 | -1.69E-46 |
| MS.gene056591 | MS.gene08616 | 0.813194 | 2.90E-51 | -1.69E-46 |
| MS.gene056688 | MS.gene08616 | 0.81165 | 6.30E-51 | -1.69E-46 |
| MS.gene056692 | MS.gene08616 | -0.802593 | 5.21E-49 | -1.69E-46 |
| MS.gene056697 | MS.gene08616 | 0.813819 | 2.11E-51 | -1.69E-46 |
| MS.gene056748 | MS.gene08616 | 0.811784 | 5.89E-51 | -1.69E-46 |
| MS.gene056856 | MS.gene08616 | 0.880736 | 4.29E-70 | -1.69E-46 |
| MS.gene056857 | MS.gene08616 | 0.851628 | 7.84E-61 | -1.69E-46 |
| MS.gene057261 | MS.gene08616 | 0.814488 | 1.50E-51 | -1.69E-46 |
| MS.gene057473 | MS.gene08616 | 0.804703 | 1.90E-49 | -1.69E-46 |
| MS.gene057544 | MS.gene08616 | 0.819849 | 9.36E-53 | -1.69E-46 |
| MS.gene057547 | MS.gene08616 | 0.818791 | 1.63E-52 | -1.69E-46 |
| MS.gene057549 | MS.gene08616 | 0.840722 | 7.33E-58 | -1.69E-46 |
| MS.gene057717 | MS.gene08616 | 0.808677 | 2.75E-50 | -1.69E-46 |
| MS.gene057768 | MS.gene08616 | 0.822823 | 1.93E-53 | -1.69E-46 |
| MS.gene058067 | MS.gene08616 | 0.834506 | 2.88E-56 | -1.69E-46 |
| MS.gene058068 | MS.gene08616 | 0.827835 | 1.26E-54 | -1.69E-46 |
| MS.gene058093 | MS.gene08616 | 0.824356 | 8.44E-54 | -1.69E-46 |
| MS.gene058099 | MS.gene08616 | 0.858307 | 9.01E-63 | -1.69E-46 |
| MS.gene058109 | MS.gene08616 | 0.801447 | 8.97E-49 | -1.69E-46 |
| MS.gene058111 | MS.gene08616 | 0.802309 | 5.96E-49 | -1.69E-46 |
| MS.gene058158 | MS.gene08616 | 0.801703 | 7.94E-49 | -1.69E-46 |
| MS.gene058171 | MS.gene08616 | 0.809466 | 1.87E-50 | -1.69E-46 |
| MS.gene058173 | MS.gene08616 | 0.808871 | 2.50E-50 | -1.69E-46 |
| MS.gene058175 | MS.gene08616 | 0.818865 | 1.57E-52 | -1.69E-46 |
| MS.gene058268 | MS.gene08616 | 0.855071 | 8.07E-62 | -1.69E-46 |
| MS.gene058269 | MS.gene08616 | 0.849445 | 3.22E-60 | -1.69E-46 |
| MS.gene058334 | MS.gene08616 | 0.805869 | 1.08E-49 | -1.69E-46 |
| MS.gene058382 | MS.gene08616 | 0.805262 | 1.45E-49 | -1.69E-46 |
| MS.gene058419 | MS.gene08616 | -0.800245 | 1.58E-48 | -1.69E-46 |
| MS.gene058603 | MS.gene08616 | 0.806259 | 8.97E-50 | -1.69E-46 |
| MS.gene059045 | MS.gene08616 | 0.803853 | 2.86E-49 | -1.69E-46 |
| MS.gene05926 | MS.gene08616 | 0.8061 | 9.69E-50 | -1.69E-46 |
| MS.gene059699 | MS.gene08616 | 0.829964 | 3.83E-55 | -1.69E-46 |
| MS.gene059703 | MS.gene08616 | 0.834658 | 2.64E-56 | -1.69E-46 |
| MS.gene059865 | MS.gene08616 | -0.819727 | 9.98E-53 | -1.69E-46 |
| MS.gene060405 | MS.gene08616 | 0.824553 | 7.59E-54 | -1.69E-46 |
| MS.gene060762 | MS.gene08616 | -0.816629 | 5.01E-52 | -1.69E-46 |
| MS.gene060823 | MS.gene08616 | 0.813789 | 2.14E-51 | -1.69E-46 |
| MS.gene060982 | MS.gene08616 | -0.813609 | 2.35E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08616.t1 | MTR_4g059730 | 93.119 | 218 | 15 | 0 | 1 | 218 | 1 | 218 | 7.32e-152 | 420 |
| MS.gene08616.t1 | MTR_1g090060 | 68.372 | 215 | 68 | 0 | 4 | 218 | 3 | 217 | 2.48e-111 | 318 |
| MS.gene08616.t1 | MTR_1g090100 | 67.290 | 214 | 70 | 0 | 5 | 218 | 3 | 216 | 3.20e-106 | 305 |
| MS.gene08616.t1 | MTR_1g090070 | 63.889 | 216 | 77 | 1 | 4 | 218 | 9 | 224 | 5.02e-103 | 297 |
| MS.gene08616.t1 | MTR_1g090090 | 65.581 | 215 | 74 | 0 | 4 | 218 | 3 | 217 | 8.41e-102 | 294 |
| MS.gene08616.t1 | MTR_1g115195 | 48.837 | 215 | 106 | 3 | 1 | 213 | 1 | 213 | 3.12e-66 | 204 |
| MS.gene08616.t1 | MTR_1g090150 | 47.867 | 211 | 109 | 1 | 3 | 213 | 2 | 211 | 4.49e-66 | 203 |
| MS.gene08616.t1 | MTR_4g019790 | 45.755 | 212 | 111 | 2 | 4 | 214 | 7 | 215 | 1.87e-62 | 194 |
| MS.gene08616.t1 | MTR_4g019780 | 46.083 | 217 | 113 | 3 | 3 | 218 | 7 | 220 | 5.74e-61 | 190 |
| MS.gene08616.t1 | MTR_7g065660 | 46.479 | 213 | 110 | 2 | 3 | 214 | 6 | 215 | 4.92e-59 | 185 |
| MS.gene08616.t1 | MTR_7g065740 | 44.393 | 214 | 114 | 2 | 3 | 214 | 6 | 216 | 5.13e-59 | 185 |
| MS.gene08616.t1 | MTR_7g065230 | 44.907 | 216 | 110 | 3 | 3 | 217 | 6 | 213 | 2.36e-57 | 181 |
| MS.gene08616.t1 | MTR_3g099757 | 45.540 | 213 | 112 | 3 | 3 | 214 | 4 | 213 | 3.54e-57 | 181 |
| MS.gene08616.t1 | MTR_7g065720 | 44.393 | 214 | 114 | 2 | 3 | 214 | 6 | 216 | 4.38e-57 | 180 |
| MS.gene08616.t1 | MTR_5g040430 | 44.545 | 220 | 116 | 4 | 2 | 216 | 5 | 223 | 4.43e-57 | 180 |
| MS.gene08616.t1 | MTR_7g065590 | 42.056 | 214 | 118 | 3 | 3 | 214 | 6 | 215 | 4.08e-55 | 175 |
| MS.gene08616.t1 | MTR_7g065750 | 42.056 | 214 | 119 | 2 | 3 | 214 | 6 | 216 | 2.21e-54 | 174 |
| MS.gene08616.t1 | MTR_8g087425 | 44.186 | 215 | 114 | 2 | 2 | 214 | 3 | 213 | 1.67e-53 | 171 |
| MS.gene08616.t1 | MTR_2g070120 | 42.254 | 213 | 118 | 3 | 1 | 212 | 1 | 209 | 4.40e-52 | 167 |
| MS.gene08616.t1 | MTR_7g065600 | 39.906 | 213 | 124 | 2 | 3 | 214 | 6 | 215 | 6.07e-52 | 167 |
| MS.gene08616.t1 | MTR_7g065270 | 42.056 | 214 | 118 | 4 | 3 | 214 | 6 | 215 | 6.84e-52 | 167 |
| MS.gene08616.t1 | MTR_8g087410 | 46.188 | 223 | 109 | 6 | 1 | 218 | 2 | 218 | 3.11e-51 | 166 |
| MS.gene08616.t1 | MTR_6g080440 | 43.939 | 198 | 105 | 3 | 18 | 213 | 17 | 210 | 4.43e-51 | 165 |
| MS.gene08616.t1 | MTR_2g070150 | 44.976 | 209 | 108 | 4 | 1 | 207 | 1 | 204 | 7.21e-51 | 164 |
| MS.gene08616.t1 | MTR_7g065630 | 40.376 | 213 | 119 | 3 | 3 | 214 | 24 | 229 | 1.93e-50 | 164 |
| MS.gene08616.t1 | MTR_7g065290 | 40.376 | 213 | 123 | 2 | 3 | 214 | 6 | 215 | 3.46e-50 | 163 |
| MS.gene08616.t1 | MTR_7g065640 | 41.784 | 213 | 102 | 3 | 3 | 214 | 6 | 197 | 4.48e-50 | 162 |
| MS.gene08616.t1 | MTR_7g065710 | 41.395 | 215 | 116 | 4 | 1 | 213 | 1 | 207 | 7.26e-50 | 162 |
| MS.gene08616.t1 | MTR_8g061950 | 43.119 | 218 | 116 | 6 | 5 | 215 | 6 | 222 | 8.44e-50 | 162 |
| MS.gene08616.t1 | MTR_7g065265 | 40.845 | 213 | 122 | 2 | 3 | 214 | 6 | 215 | 2.99e-49 | 160 |
| MS.gene08616.t1 | MTR_7g065700 | 42.056 | 214 | 116 | 3 | 1 | 213 | 4 | 210 | 7.46e-49 | 159 |
| MS.gene08616.t1 | MTR_2g070200 | 42.342 | 222 | 119 | 6 | 1 | 218 | 1 | 217 | 1.52e-48 | 159 |
| MS.gene08616.t1 | MTR_7g065260 | 43.256 | 215 | 115 | 5 | 3 | 214 | 13 | 223 | 2.28e-47 | 156 |
| MS.gene08616.t1 | MTR_2g070130 | 40.191 | 209 | 120 | 3 | 1 | 208 | 1 | 205 | 3.90e-47 | 155 |
| MS.gene08616.t1 | MTR_2g070180 | 41.905 | 210 | 115 | 4 | 1 | 208 | 1 | 205 | 5.55e-47 | 154 |
| MS.gene08616.t1 | MTR_2g070210 | 40.278 | 216 | 120 | 5 | 1 | 212 | 1 | 211 | 8.83e-46 | 151 |
| MS.gene08616.t1 | MTR_2g070140 | 42.647 | 204 | 110 | 4 | 17 | 218 | 1 | 199 | 2.52e-45 | 150 |
| MS.gene08616.t1 | MTR_7g065680 | 39.906 | 213 | 120 | 3 | 3 | 214 | 6 | 211 | 1.13e-44 | 149 |
| MS.gene08616.t1 | MTR_8g056940 | 39.437 | 213 | 124 | 3 | 1 | 212 | 1 | 209 | 1.39e-43 | 145 |
| MS.gene08616.t1 | MTR_3g467420 | 40.000 | 210 | 121 | 3 | 10 | 218 | 15 | 220 | 6.36e-43 | 144 |
| MS.gene08616.t1 | MTR_2g070070 | 40.376 | 213 | 122 | 3 | 1 | 212 | 1 | 209 | 3.86e-42 | 142 |
| MS.gene08616.t1 | MTR_5g037380 | 37.778 | 225 | 131 | 4 | 3 | 218 | 4 | 228 | 4.04e-42 | 142 |
| MS.gene08616.t1 | MTR_4g124130 | 39.545 | 220 | 127 | 4 | 1 | 218 | 1 | 216 | 1.17e-41 | 141 |
| MS.gene08616.t1 | MTR_3g467430 | 39.171 | 217 | 127 | 3 | 3 | 218 | 8 | 220 | 1.22e-41 | 141 |
| MS.gene08616.t1 | MTR_2g070060 | 37.963 | 216 | 129 | 3 | 1 | 215 | 1 | 212 | 1.66e-41 | 140 |
| MS.gene08616.t1 | MTR_2g070110 | 41.232 | 211 | 114 | 5 | 1 | 208 | 1 | 204 | 1.24e-40 | 138 |
| MS.gene08616.t1 | MTR_5g076900 | 35.849 | 212 | 131 | 3 | 2 | 212 | 4 | 211 | 1.01e-36 | 128 |
| MS.gene08616.t1 | MTR_0186s0030 | 46.324 | 136 | 72 | 1 | 1 | 135 | 1 | 136 | 1.39e-35 | 123 |
| MS.gene08616.t1 | MTR_2g072120 | 60.714 | 56 | 21 | 1 | 48 | 102 | 2 | 57 | 2.33e-19 | 79.3 |
| MS.gene08616.t1 | MTR_5g037390 | 42.857 | 91 | 42 | 2 | 28 | 115 | 11 | 94 | 1.06e-13 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08616.t1 | AT3G09270 | 45.581 | 215 | 113 | 3 | 3 | 215 | 5 | 217 | 5.22e-59 | 185 |
| MS.gene08616.t1 | AT2G29450 | 43.578 | 218 | 119 | 3 | 3 | 218 | 5 | 220 | 1.70e-56 | 179 |
| MS.gene08616.t1 | AT2G29460 | 43.318 | 217 | 120 | 2 | 3 | 218 | 6 | 220 | 3.02e-56 | 178 |
| MS.gene08616.t1 | AT2G29490 | 43.981 | 216 | 118 | 2 | 3 | 217 | 6 | 219 | 7.27e-56 | 177 |
| MS.gene08616.t1 | AT2G29470 | 42.661 | 218 | 121 | 3 | 3 | 218 | 6 | 221 | 6.23e-55 | 175 |
| MS.gene08616.t1 | AT2G29420 | 40.092 | 217 | 128 | 1 | 2 | 218 | 7 | 221 | 1.30e-54 | 174 |
| MS.gene08616.t1 | AT2G29480 | 43.779 | 217 | 119 | 2 | 3 | 218 | 6 | 220 | 3.86e-54 | 173 |
| MS.gene08616.t1 | AT1G10370 | 43.243 | 222 | 121 | 3 | 2 | 218 | 3 | 224 | 5.56e-54 | 172 |
| MS.gene08616.t1 | AT1G17180 | 41.096 | 219 | 124 | 3 | 1 | 218 | 1 | 215 | 3.53e-49 | 160 |
| MS.gene08616.t1 | AT2G29440 | 40.092 | 217 | 127 | 2 | 3 | 218 | 5 | 219 | 9.10e-49 | 159 |
| MS.gene08616.t1 | AT1G78340 | 41.509 | 212 | 119 | 3 | 1 | 211 | 1 | 208 | 1.78e-47 | 155 |
| MS.gene08616.t1 | AT1G74590 | 39.269 | 219 | 130 | 2 | 3 | 218 | 6 | 224 | 4.38e-47 | 155 |
| MS.gene08616.t1 | AT1G27130 | 40.092 | 217 | 124 | 4 | 3 | 213 | 5 | 221 | 1.26e-46 | 154 |
| MS.gene08616.t1 | AT1G10360 | 40.724 | 221 | 126 | 3 | 3 | 218 | 4 | 224 | 1.30e-46 | 154 |
| MS.gene08616.t1 | AT1G78320 | 40.566 | 212 | 117 | 6 | 1 | 209 | 1 | 206 | 1.93e-46 | 153 |
| MS.gene08616.t1 | AT5G62480 | 36.404 | 228 | 130 | 5 | 1 | 217 | 5 | 228 | 2.35e-45 | 151 |
| MS.gene08616.t1 | AT1G78370 | 39.151 | 212 | 124 | 3 | 1 | 211 | 1 | 208 | 5.66e-45 | 149 |
| MS.gene08616.t1 | AT1G59670 | 39.631 | 217 | 125 | 4 | 3 | 213 | 5 | 221 | 7.24e-45 | 149 |
| MS.gene08616.t1 | AT1G69930 | 37.209 | 215 | 131 | 3 | 3 | 213 | 11 | 225 | 8.18e-44 | 147 |
| MS.gene08616.t1 | AT1G27140 | 39.462 | 223 | 128 | 4 | 3 | 218 | 5 | 227 | 2.11e-43 | 146 |
| MS.gene08616.t1 | AT1G17170 | 39.906 | 213 | 122 | 4 | 1 | 212 | 1 | 208 | 4.30e-43 | 144 |
| MS.gene08616.t1 | AT1G78380 | 39.269 | 219 | 128 | 3 | 1 | 218 | 1 | 215 | 5.49e-43 | 144 |
| MS.gene08616.t1 | AT1G59700 | 37.327 | 217 | 130 | 4 | 3 | 213 | 5 | 221 | 2.94e-42 | 143 |
| MS.gene08616.t1 | AT1G17190 | 35.945 | 217 | 134 | 3 | 3 | 218 | 4 | 216 | 2.47e-41 | 140 |
| MS.gene08616.t1 | AT1G53680 | 38.119 | 202 | 118 | 4 | 10 | 208 | 13 | 210 | 4.41e-38 | 132 |
| MS.gene08616.t1 | AT1G78360 | 36.916 | 214 | 129 | 4 | 1 | 212 | 1 | 210 | 1.44e-37 | 130 |
| MS.gene08616.t1 | AT1G69920 | 35.349 | 215 | 133 | 4 | 5 | 213 | 35 | 249 | 2.54e-37 | 130 |
| MS.gene08616.t1 | AT3G43800 | 37.727 | 220 | 128 | 5 | 3 | 217 | 4 | 219 | 8.24e-37 | 129 |
| MS.gene08616.t1 | AT5G62480 | 30.222 | 225 | 117 | 5 | 3 | 217 | 7 | 201 | 2.95e-29 | 108 |
| MS.gene08616.t1 | AT5G62480 | 30.222 | 225 | 118 | 5 | 3 | 217 | 7 | 202 | 4.03e-29 | 108 |
| MS.gene08616.t1 | AT1G78320 | 32.701 | 211 | 108 | 6 | 1 | 209 | 1 | 179 | 9.81e-28 | 104 |
Find 35 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAGCCCAAGCTCGGTTC+TGG | 0.280479 | 4.1:-39003005 | MS.gene08616:CDS |
| AGGACACACCGTTAACATTC+TGG | 0.283468 | 4.1:-39002539 | MS.gene08616:CDS |
| AAGTGAAGCTACATGGATTT+TGG | 0.302285 | 4.1:-39003263 | MS.gene08616:CDS |
| AATACTACCCTCAAAGTAAA+AGG | 0.302524 | 4.1:+39003234 | None:intergenic |
| CCGAGCTTGGGCTCTCTCAT+AGG | 0.329353 | 4.1:+39003011 | None:intergenic |
| AGAATACCATAGCGAAAATC+TGG | 0.331408 | 4.1:-39002618 | MS.gene08616:CDS |
| GTATTGACCCTTAAGCTAAA+AGG | 0.363440 | 4.1:-39003216 | MS.gene08616:CDS |
| TTGAATACATTGATGAGATA+TGG | 0.380718 | 4.1:-39003062 | MS.gene08616:CDS |
| TCTTCTTCTATGTTCTCATA+TGG | 0.416671 | 4.1:+39003189 | None:intergenic |
| AACATTCTGGACATAGCTCT+TGG | 0.422694 | 4.1:-39002526 | MS.gene08616:CDS |
| AGCTATGTCCAGAATGTTAA+CGG | 0.440075 | 4.1:+39002531 | None:intergenic |
| GCTATCAGTTTCTCATGATC+AGG | 0.440168 | 4.1:+39002373 | None:intergenic |
| GCTCTCTCATAGGGATCAAC+AGG | 0.444906 | 4.1:+39003021 | None:intergenic |
| AATTCCCTCACTTGCATGCA+TGG | 0.474769 | 4.1:-39002438 | MS.gene08616:CDS |
| TTCAAGAATGTACCTGTCAT+AGG | 0.475029 | 4.1:-39002406 | MS.gene08616:CDS |
| GAGAGCCCAAGCTCGGTTCT+GGG | 0.483641 | 4.1:-39003004 | MS.gene08616:CDS |
| CGAGCTTGGGCTCTCTCATA+GGG | 0.521012 | 4.1:+39003012 | None:intergenic |
| GATTGTATTGAAGAAGTTGA+GGG | 0.534705 | 4.1:+39003151 | None:intergenic |
| TCAAACCATGCATGCAAGTG+AGG | 0.535813 | 4.1:+39002433 | None:intergenic |
| ACTCCAGTGTTAGTTCATGA+TGG | 0.542419 | 4.1:-39003114 | MS.gene08616:CDS |
| GCAGATCAAGTGAAGCTACA+TGG | 0.559561 | 4.1:-39003270 | MS.gene08616:CDS |
| TTTCCATCATGAACTAACAC+TGG | 0.567459 | 4.1:+39003111 | None:intergenic |
| GAGGGAATTTCTCAGCTGCT+AGG | 0.567585 | 4.1:+39002452 | None:intergenic |
| CAAACCATGCATGCAAGTGA+GGG | 0.568010 | 4.1:+39002434 | None:intergenic |
| GGATTGTATTGAAGAAGTTG+AGG | 0.571991 | 4.1:+39003150 | None:intergenic |
| TAGGGATCAACAGGAAGCAA+AGG | 0.573576 | 4.1:+39003030 | None:intergenic |
| AGGGATCAACAGGAAGCAAA+GGG | 0.577972 | 4.1:+39003031 | None:intergenic |
| CTAACGAGTGCACCAACTGC+AGG | 0.579048 | 4.1:+39002664 | None:intergenic |
| GAATACCATAGCGAAAATCT+GGG | 0.582158 | 4.1:-39002617 | MS.gene08616:CDS |
| GGTGCACTCGTTAGTAGCGG+TGG | 0.611355 | 4.1:-39002655 | MS.gene08616:CDS |
| CCTATGAGAGAGCCCAAGCT+CGG | 0.617875 | 4.1:-39003011 | MS.gene08616:CDS |
| ATGATCATAGACTCACATAG+AGG | 0.640551 | 4.1:+39003087 | None:intergenic |
| GTAGCGGTGGCGATGAAGAG+CGG | 0.649339 | 4.1:-39002642 | MS.gene08616:CDS |
| GTTGGTGCACTCGTTAGTAG+CGG | 0.664719 | 4.1:-39002658 | MS.gene08616:CDS |
| ACAGGAAGCAAAGGGTACTG+TGG | 0.666663 | 4.1:+39003039 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTAATCAACATATTATTT+AGG | - | chr4.1:39002767-39002786 | MS.gene08616:intron | 10.0% |
| !! | TTATCATATTGAATAAATTT+AGG | + | chr4.1:39002890-39002909 | None:intergenic | 10.0% |
| !! | AAAAATAATATTGTTGGATA+TGG | + | chr4.1:39002844-39002863 | None:intergenic | 15.0% |
| !!! | AAATTACTATTACTTTTTCA+TGG | + | chr4.1:39002704-39002723 | None:intergenic | 15.0% |
| ! | CTAAGAAAATTTGCAAATGA+AGG | + | chr4.1:39002806-39002825 | None:intergenic | 25.0% |
| ! | TTGAATACATTGATGAGATA+TGG | - | chr4.1:39002556-39002575 | MS.gene08616:CDS | 25.0% |
| AATACTACCCTCAAAGTAAA+AGG | + | chr4.1:39002387-39002406 | None:intergenic | 30.0% | |
| GATTGTATTGAAGAAGTTGA+GGG | + | chr4.1:39002470-39002489 | None:intergenic | 30.0% | |
| TCTTCTTCTATGTTCTCATA+TGG | + | chr4.1:39002432-39002451 | None:intergenic | 30.0% | |
| ! | GAAACTGATAGCTTTTATGA+AGG | - | chr4.1:39003256-39003275 | MS.gene08616:CDS | 30.0% |
| ! | GACTACAAGAAGAAACTTTT+TGG | - | chr4.1:39003056-39003075 | MS.gene08616:CDS | 30.0% |
| ! | TACAAGAAGAAACTTTTTGG+AGG | - | chr4.1:39003059-39003078 | MS.gene08616:CDS | 30.0% |
| ! | TTTTCATGGTATGCAAATGA+AGG | + | chr4.1:39002690-39002709 | None:intergenic | 30.0% |
| ! | TTTTTATAGATTCCTGCAGT+TGG | - | chr4.1:39002942-39002961 | MS.gene08616:intron | 30.0% |
| !!! | ATGGTATACCTTTTAGCTTA+AGG | + | chr4.1:39002413-39002432 | None:intergenic | 30.0% |
| !!! | TGGTATACCTTTTAGCTTAA+GGG | + | chr4.1:39002412-39002431 | None:intergenic | 30.0% |
| AGAATACCATAGCGAAAATC+TGG | - | chr4.1:39003000-39003019 | MS.gene08616:CDS | 35.0% | |
| AGCTATGTCCAGAATGTTAA+CGG | + | chr4.1:39003090-39003109 | None:intergenic | 35.0% | |
| ATGATCATAGACTCACATAG+AGG | + | chr4.1:39002534-39002553 | None:intergenic | 35.0% | |
| GAATACCATAGCGAAAATCT+GGG | - | chr4.1:39003001-39003020 | MS.gene08616:CDS | 35.0% | |
| GGATATGGTATCAACTATCA+AGG | + | chr4.1:39002829-39002848 | None:intergenic | 35.0% | |
| GGATTGTATTGAAGAAGTTG+AGG | + | chr4.1:39002471-39002490 | None:intergenic | 35.0% | |
| TTCAAGAATGTACCTGTCAT+AGG | - | chr4.1:39003212-39003231 | MS.gene08616:CDS | 35.0% | |
| TTTCCATCATGAACTAACAC+TGG | + | chr4.1:39002510-39002529 | None:intergenic | 35.0% | |
| ! | AAGTGAAGCTACATGGATTT+TGG | - | chr4.1:39002355-39002374 | MS.gene08616:CDS | 35.0% |
| ! | GTACAGTCCTTTTACTTTGA+GGG | - | chr4.1:39002377-39002396 | MS.gene08616:CDS | 35.0% |
| ! | GTATTGACCCTTAAGCTAAA+AGG | - | chr4.1:39002402-39002421 | MS.gene08616:CDS | 35.0% |
| AACATTCTGGACATAGCTCT+TGG | - | chr4.1:39003092-39003111 | MS.gene08616:CDS | 40.0% | |
| ACTCCAGTGTTAGTTCATGA+TGG | - | chr4.1:39002504-39002523 | MS.gene08616:CDS | 40.0% | |
| GCTATCAGTTTCTCATGATC+AGG | + | chr4.1:39003248-39003267 | None:intergenic | 40.0% | |
| ! | ATGTTCCCAGATTTTCGCTA+TGG | + | chr4.1:39003009-39003028 | None:intergenic | 40.0% |
| ! | GGAAGATTTTCACCTATGAC+AGG | + | chr4.1:39003227-39003246 | None:intergenic | 40.0% |
| ! | GGTACAGTCCTTTTACTTTG+AGG | - | chr4.1:39002376-39002395 | MS.gene08616:CDS | 40.0% |
| AATTCCCTCACTTGCATGCA+TGG | - | chr4.1:39003180-39003199 | MS.gene08616:CDS | 45.0% | |
| AGGACACACCGTTAACATTC+TGG | - | chr4.1:39003079-39003098 | MS.gene08616:CDS | 45.0% | |
| AGGGATCAACAGGAAGCAAA+GGG | + | chr4.1:39002590-39002609 | None:intergenic | 45.0% | |
| CAAACCATGCATGCAAGTGA+GGG | + | chr4.1:39003187-39003206 | None:intergenic | 45.0% | |
| GCAGATCAAGTGAAGCTACA+TGG | - | chr4.1:39002348-39002367 | MS.gene08616:CDS | 45.0% | |
| TAGGGATCAACAGGAAGCAA+AGG | + | chr4.1:39002591-39002610 | None:intergenic | 45.0% | |
| TCAAACCATGCATGCAAGTG+AGG | + | chr4.1:39003188-39003207 | None:intergenic | 45.0% | |
| ! | ACTGGAGTTTTCTTGTGCAC+TGG | + | chr4.1:39002492-39002511 | None:intergenic | 45.0% |
| ! | ATTTTACCCAGAACCGAGCT+TGG | + | chr4.1:39002623-39002642 | None:intergenic | 45.0% |
| ! | TTTTACCCAGAACCGAGCTT+GGG | + | chr4.1:39002622-39002641 | None:intergenic | 45.0% |
| !! | AGTATTATAAATAATTTATT+GGG | - | chr4.1:39002915-39002934 | MS.gene08616:intron | 5.0% |
| !! | ATAAAAAAAAATAATATTGT+TGG | + | chr4.1:39002850-39002869 | None:intergenic | 5.0% |
| !! | TAGTATTATAAATAATTTAT+TGG | - | chr4.1:39002914-39002933 | MS.gene08616:intron | 5.0% |
| GAGGGAATTTCTCAGCTGCT+AGG | + | chr4.1:39003169-39003188 | None:intergenic | 50.0% | |
| GCTCTCTCATAGGGATCAAC+AGG | + | chr4.1:39002600-39002619 | None:intergenic | 50.0% | |
| GTTGGTGCACTCGTTAGTAG+CGG | - | chr4.1:39002960-39002979 | MS.gene08616:intron | 50.0% | |
| !! | ACAGGAAGCAAAGGGTACTG+TGG | + | chr4.1:39002582-39002601 | None:intergenic | 50.0% |
| CCTATGAGAGAGCCCAAGCT+CGG | - | chr4.1:39002607-39002626 | MS.gene08616:CDS | 55.0% | |
| CGAGCTTGGGCTCTCTCATA+GGG | + | chr4.1:39002609-39002628 | None:intergenic | 55.0% | |
| CTAACGAGTGCACCAACTGC+AGG | + | chr4.1:39002957-39002976 | None:intergenic | 55.0% | |
| AGAGAGCCCAAGCTCGGTTC+TGG | - | chr4.1:39002613-39002632 | MS.gene08616:CDS | 60.0% | |
| CCGAGCTTGGGCTCTCTCAT+AGG | + | chr4.1:39002610-39002629 | None:intergenic | 60.0% | |
| GAGAGCCCAAGCTCGGTTCT+GGG | - | chr4.1:39002614-39002633 | MS.gene08616:CDS | 60.0% | |
| GGTGCACTCGTTAGTAGCGG+TGG | - | chr4.1:39002963-39002982 | MS.gene08616:intron | 60.0% | |
| GTAGCGGTGGCGATGAAGAG+CGG | - | chr4.1:39002976-39002995 | MS.gene08616:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 39002345 | 39003295 | 39002345 | ID=MS.gene08616 |
| chr4.1 | mRNA | 39002345 | 39003295 | 39002345 | ID=MS.gene08616.t1;Parent=MS.gene08616 |
| chr4.1 | exon | 39002984 | 39003295 | 39002984 | ID=MS.gene08616.t1.exon1;Parent=MS.gene08616.t1 |
| chr4.1 | CDS | 39002984 | 39003295 | 39002984 | ID=cds.MS.gene08616.t1;Parent=MS.gene08616.t1 |
| chr4.1 | exon | 39002345 | 39002689 | 39002345 | ID=MS.gene08616.t1.exon2;Parent=MS.gene08616.t1 |
| chr4.1 | CDS | 39002345 | 39002689 | 39002345 | ID=cds.MS.gene08616.t1;Parent=MS.gene08616.t1 |
| Gene Sequence |
| Protein sequence |
