AlfalfaGEDB | MS.gene28659

M. sativa cultivar XinJiangDaYe / MS.gene28659

KEGG: K12456
GO: GO:0000151 GO:0000152 GO:0005575 GO:0005680 GO:0031461 GO:0032991 GO:1902494 GO:1990234
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28659.t1	XP_003626105.1	97.8	46	1	0	1	46	1	46	6.10E-16	92.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28659.t1	Q8L981	78.3	46	10	0	1	46	1	46	1.3e-14	79.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28659.t1	Q2HV02	97.8	46	1	0	1	46	1	46	4.4e-16	92.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene28659	0.826765	2.27E-54	-1.69E-46
MS.gene049404	MS.gene28659	0.802855	4.60E-49	-1.69E-46
MS.gene049435	MS.gene28659	0.805772	1.14E-49	-1.69E-46
MS.gene049501	MS.gene28659	0.830726	2.50E-55	-1.69E-46
MS.gene049647	MS.gene28659	0.801383	9.24E-49	-1.69E-46
MS.gene050442	MS.gene28659	0.824361	8.42E-54	-1.69E-46
MS.gene050750	MS.gene28659	0.807747	4.35E-50	-1.69E-46
MS.gene050826	MS.gene28659	0.804687	1.92E-49	-1.69E-46
MS.gene050953	MS.gene28659	0.816714	4.80E-52	-1.69E-46
MS.gene051198	MS.gene28659	0.814489	1.50E-51	-1.69E-46
MS.gene051247	MS.gene28659	0.81298	3.23E-51	-1.69E-46
MS.gene051265	MS.gene28659	0.810407	1.17E-50	-1.69E-46
MS.gene051710	MS.gene28659	0.808344	3.24E-50	-1.69E-46
MS.gene051719	MS.gene28659	0.806516	7.92E-50	-1.69E-46
MS.gene051800	MS.gene28659	0.822821	1.93E-53	-1.69E-46
MS.gene052020	MS.gene28659	0.805445	1.33E-49	-1.69E-46
MS.gene052333	MS.gene28659	0.801033	1.09E-48	-1.69E-46
MS.gene052863	MS.gene28659	0.809406	1.92E-50	-1.69E-46
MS.gene052867	MS.gene28659	0.821066	4.92E-53	-1.69E-46
MS.gene05288	MS.gene28659	0.820207	7.75E-53	-1.69E-46
MS.gene052896	MS.gene28659	0.811029	8.59E-51	-1.69E-46
MS.gene05289	MS.gene28659	0.836719	7.93E-57	-1.69E-46
MS.gene053166	MS.gene28659	0.818791	1.63E-52	-1.69E-46
MS.gene053221	MS.gene28659	0.831719	1.42E-55	-1.69E-46
MS.gene053584	MS.gene28659	0.814742	1.32E-51	-1.69E-46
MS.gene05414	MS.gene28659	0.826683	2.37E-54	-1.69E-46
MS.gene05415	MS.gene28659	0.821967	3.05E-53	-1.69E-46
MS.gene05417	MS.gene28659	0.80717	5.76E-50	-1.69E-46
MS.gene054236	MS.gene28659	0.856889	2.37E-62	-1.69E-46
MS.gene054405	MS.gene28659	0.811056	8.47E-51	-1.69E-46
MS.gene054467	MS.gene28659	0.842348	2.73E-58	-1.69E-46
MS.gene054468	MS.gene28659	0.80496	1.68E-49	-1.69E-46
MS.gene054472	MS.gene28659	0.825838	3.77E-54	-1.69E-46
MS.gene054559	MS.gene28659	0.817491	3.21E-52	-1.69E-46
MS.gene05468	MS.gene28659	0.806359	8.55E-50	-1.69E-46
MS.gene054775	MS.gene28659	0.824001	1.02E-53	-1.69E-46
MS.gene054801	MS.gene28659	0.823761	1.16E-53	-1.69E-46
MS.gene055227	MS.gene28659	0.800827	1.20E-48	-1.69E-46
MS.gene055228	MS.gene28659	0.804906	1.73E-49	-1.69E-46
MS.gene055356	MS.gene28659	0.817659	2.94E-52	-1.69E-46
MS.gene055357	MS.gene28659	0.829039	6.43E-55	-1.69E-46
MS.gene055466	MS.gene28659	0.819446	1.16E-52	-1.69E-46
MS.gene055608	MS.gene28659	0.815479	9.05E-52	-1.69E-46
MS.gene055701	MS.gene28659	0.802363	5.81E-49	-1.69E-46
MS.gene055818	MS.gene28659	0.82665	2.41E-54	-1.69E-46
MS.gene055875	MS.gene28659	0.811503	6.78E-51	-1.69E-46
MS.gene055926	MS.gene28659	0.852456	4.56E-61	-1.69E-46
MS.gene05604	MS.gene28659	0.820376	7.09E-53	-1.69E-46
MS.gene056173	MS.gene28659	0.829633	4.61E-55	-1.69E-46
MS.gene056784	MS.gene28659	0.803192	3.92E-49	-1.69E-46
MS.gene057444	MS.gene28659	0.805352	1.39E-49	-1.69E-46
MS.gene057869	MS.gene28659	0.802466	5.54E-49	-1.69E-46
MS.gene057915	MS.gene28659	0.802636	5.11E-49	-1.69E-46
MS.gene058010	MS.gene28659	0.819801	9.60E-53	-1.69E-46
MS.gene058037	MS.gene28659	0.801307	9.58E-49	-1.69E-46
MS.gene058515	MS.gene28659	0.80983	1.56E-50	-1.69E-46
MS.gene058625	MS.gene28659	0.828801	7.35E-55	-1.69E-46
MS.gene059128	MS.gene28659	0.802518	5.40E-49	-1.69E-46
MS.gene059272	MS.gene28659	0.804873	1.75E-49	-1.69E-46
MS.gene059382	MS.gene28659	0.807512	4.88E-50	-1.69E-46
MS.gene059584	MS.gene28659	0.803427	3.50E-49	-1.69E-46
MS.gene060011	MS.gene28659	0.826521	2.59E-54	-1.69E-46
MS.gene060137	MS.gene28659	-0.837894	3.97E-57	-1.69E-46
MS.gene060167	MS.gene28659	0.829125	6.13E-55	-1.69E-46
MS.gene060312	MS.gene28659	0.801378	9.26E-49	-1.69E-46
MS.gene060322	MS.gene28659	0.835771	1.38E-56	-1.69E-46
MS.gene06052	MS.gene28659	0.821541	3.82E-53	-1.69E-46
MS.gene060802	MS.gene28659	0.817383	3.39E-52	-1.69E-46
MS.gene060803	MS.gene28659	0.819948	8.88E-53	-1.69E-46
MS.gene060998	MS.gene28659	0.815628	8.39E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28659.t1	MTR_7g111090	91.837	49	4	0	1	49	1	49	6.61e-26	89.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28659.t1	AT1G73177	73.469	49	13	0	1	49	1	49	8.10e-11	51.2

Find 10 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCCTCCAACACTGGTTGTT+CGG	0.370391	7.1:-3992008	MS.gene28659:CDS
AAAATGGCAGAACTGAGTTT+GGG	0.446438	7.1:-3992610	MS.gene28659:CDS
TTGACATTGTTGATGAGGAT+TGG	0.447381	7.1:-3992579	MS.gene28659:CDS
ACAACCAGTGTTGGAGGCAG+TGG	0.463826	7.1:+3992012	None:intergenic
TCTTCCACTGCCTCCAACAC+TGG	0.491395	7.1:-3992016	MS.gene28659:CDS
TCTAATTGACATTGTTGATG+AGG	0.512652	7.1:-3992584	MS.gene28659:CDS
GTCCGAACAACCAGTGTTGG+AGG	0.546833	7.1:+3992006	None:intergenic
AGAGACATTCTTCCTCAAGA+TGG	0.570997	7.1:-3992553	MS.gene28659:intron
TCTGTCCGAACAACCAGTGT+TGG	0.577627	7.1:+3992003	None:intergenic
TGTTCGGACAGATGATACTG+AGG	0.734610	7.1:-3991992	MS.gene28659:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTTATCAATATACAATAT+AGG	+	chr7.1:3991807-3991826	None:intergenic	10.0%
!!	AAAAAAAAAATACCATCTTG+AGG	+	chr7.1:3991507-3991526	None:intergenic	20.0%
!!	AAAAAACAGTTGCAAAAAAA+AGG	-	chr7.1:3991629-3991648	MS.gene28659:intron	20.0%
!!	CAAATATAACAAAGTATAGA+TGG	-	chr7.1:3991872-3991891	MS.gene28659:intron	20.0%
!!!	ACTTCTTATTTCATTTACAA+TGG	+	chr7.1:3991703-3991722	None:intergenic	20.0%
!!!	ATGTTTTCAAACTTATATGT+CGG	-	chr7.1:3992469-3992488	MS.gene28659:intron	20.0%
!	ATCAATATACAATATAGGAC+AGG	+	chr7.1:3991802-3991821	None:intergenic	25.0%
!	ATTGATTATTGTTCATGAGA+TGG	-	chr7.1:3992515-3992534	MS.gene28659:intron	25.0%
!	CCAACTACAAATAGATTATT+CGG	-	chr7.1:3991594-3991613	MS.gene28659:intron	25.0%
!	GTTTAAAGAAAATGTGTTCT+TGG	-	chr7.1:3992289-3992308	MS.gene28659:intron	25.0%
!	TATAACAAAGTATAGATGGT+TGG	-	chr7.1:3991876-3991895	MS.gene28659:intron	25.0%
!	TCAATATACAATATAGGACA+GGG	+	chr7.1:3991801-3991820	None:intergenic	25.0%
!	TGTAAATTAGTATACACTGA+CGG	+	chr7.1:3991568-3991587	None:intergenic	25.0%
!	TTGATTATTGTTCATGAGAT+GGG	-	chr7.1:3992516-3992535	MS.gene28659:intron	25.0%
!!	CTAAATTGCAACTTTTTGAT+TGG	+	chr7.1:3992241-3992260	None:intergenic	25.0%
!!	TGTTTTCAAACTTATATGTC+GGG	-	chr7.1:3992470-3992489	MS.gene28659:intron	25.0%
	AAAAAGGCTACATAGCAAAA+TGG	-	chr7.1:3991645-3991664	MS.gene28659:intron	30.0%
	AACTATATATGTTGCCATGT+TGG	-	chr7.1:3992414-3992433	MS.gene28659:intron	30.0%
	ACGAAGAGAATGTAAGTTTA+GGG	-	chr7.1:3991920-3991939	MS.gene28659:intron	30.0%
	CCGAATAATCTATTTGTAGT+TGG	+	chr7.1:3991597-3991616	None:intergenic	30.0%
	CTGATTAACAACAAATCACA+TGG	+	chr7.1:3992590-3992609	None:intergenic	30.0%
	GGCTACATAACTTATAATAG+TGG	-	chr7.1:3992492-3992511	MS.gene28659:intron	30.0%
	TAAACTAAGCTCAAGAAAGA+TGG	+	chr7.1:3992128-3992147	None:intergenic	30.0%
	TACGAAGAGAATGTAAGTTT+AGG	-	chr7.1:3991919-3991938	MS.gene28659:intron	30.0%
	TCTAATTGACATTGTTGATG+AGG	-	chr7.1:3991461-3991480	MS.gene28659:intron	30.0%
!	ACAACCATGTTTTCATTGAA+AGG	+	chr7.1:3992271-3992290	None:intergenic	30.0%
!	GTTTTCAAACTTATATGTCG+GGG	-	chr7.1:3992471-3992490	MS.gene28659:intron	30.0%
!	TTGATAATTGATATCCACGT+GGG	-	chr7.1:3991974-3991993	MS.gene28659:intron	30.0%
!!	GATTTGTTGTTAATCAGATC+AGG	-	chr7.1:3992593-3992612	MS.gene28659:CDS	30.0%
	AATCTATTTGTAGTTGGCGT+TGG	+	chr7.1:3991591-3991610	None:intergenic	35.0%
	AGAGAATGTAAGTTTAGGGA+TGG	-	chr7.1:3991924-3991943	MS.gene28659:intron	35.0%
	AGTTTAGGGATGGATGAAAT+TGG	-	chr7.1:3991934-3991953	MS.gene28659:intron	35.0%
	ATACAATATAGGACAGGGAT+GGG	+	chr7.1:3991796-3991815	None:intergenic	35.0%
	GACTAAAGCAGTTACATTTG+TGG	-	chr7.1:3992099-3992118	MS.gene28659:intron	35.0%
	GATAAATTGTCCTGAACTGA+CGG	-	chr7.1:3991819-3991838	MS.gene28659:intron	35.0%
	TATACAATATAGGACAGGGA+TGG	+	chr7.1:3991797-3991816	None:intergenic	35.0%
	TGTGCCTTTCAATGAAAACA+TGG	-	chr7.1:3992264-3992283	MS.gene28659:intron	35.0%
!	CTTGATAATTGATATCCACG+TGG	-	chr7.1:3991973-3991992	MS.gene28659:intron	35.0%
!	GTATTAAGCACACGTTGATT+TGG	-	chr7.1:3992161-3992180	MS.gene28659:intron	35.0%
!	TTGACATTGTTGATGAGGAT+TGG	-	chr7.1:3991466-3991485	MS.gene28659:intron	35.0%
	ACAATGCATTACTGACAGTG+TGG	-	chr7.1:3992344-3992363	MS.gene28659:intron	40.0%
	ACAGTGTGGCATGTAAACAA+CGG	-	chr7.1:3992358-3992377	MS.gene28659:intron	40.0%
	AGAGACATTCTTCCTCAAGA+TGG	-	chr7.1:3991492-3991511	MS.gene28659:intron	40.0%
	ATATGTTGCCATGTTGGTGT+CGG	-	chr7.1:3992420-3992439	MS.gene28659:intron	40.0%
	ATCAGATTTACATTCCCACG+TGG	+	chr7.1:3991991-3992010	None:intergenic	40.0%
	CAAATATGTACCGTCAGTTC+AGG	+	chr7.1:3991832-3991851	None:intergenic	40.0%
!	TCATGAGATGGGTCTTATGT+TGG	-	chr7.1:3992527-3992546	MS.gene28659:intron	40.0%
	ACAATGGTTATGCACGCTGT+TGG	+	chr7.1:3991687-3991706	None:intergenic	45.0%
	CAATGGTTATGCACGCTGTT+GGG	+	chr7.1:3991686-3991705	None:intergenic	45.0%
	TGTCGGATACAGACACATGT+TGG	-	chr7.1:3992437-3992456	MS.gene28659:intron	45.0%
	TGTTCGGACAGATGATACTG+AGG	-	chr7.1:3992053-3992072	MS.gene28659:intron	45.0%
!	TGAGATGGGTCTTATGTTGG+AGG	-	chr7.1:3992530-3992549	MS.gene28659:intron	45.0%
!!	AGCACACGTTGATTTGGTGA+TGG	-	chr7.1:3992167-3992186	MS.gene28659:intron	45.0%
!!	GCACACGTTGATTTGGTGAT+GGG	-	chr7.1:3992168-3992187	MS.gene28659:intron	45.0%
	GTCTGTATCCGACACCAACA+TGG	+	chr7.1:3992431-3992450	None:intergenic	50.0%
	TCTGTCCGAACAACCAGTGT+TGG	+	chr7.1:3992045-3992064	None:intergenic	50.0%
	TGCACGCTGTTGGGTCTAAT+TGG	+	chr7.1:3991677-3991696	None:intergenic	50.0%
!	TGCCTCCAACACTGGTTGTT+CGG	-	chr7.1:3992037-3992056	MS.gene28659:intron	50.0%
	TCTTCCACTGCCTCCAACAC+TGG	-	chr7.1:3992029-3992048	MS.gene28659:intron	55.0%
!	ACAACCAGTGTTGGAGGCAG+TGG	+	chr7.1:3992036-3992055	None:intergenic	55.0%
!	GTCCGAACAACCAGTGTTGG+AGG	+	chr7.1:3992042-3992061	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	3991438	3992629	3991438	ID=MS.gene28659
chr7.1	mRNA	3991438	3992629	3991438	ID=MS.gene28659.t1;Parent=MS.gene28659
chr7.1	exon	3992554	3992629	3992554	ID=MS.gene28659.t1.exon1;Parent=MS.gene28659.t1
chr7.1	CDS	3992554	3992629	3992554	ID=cds.MS.gene28659.t1;Parent=MS.gene28659.t1
chr7.1	exon	3991986	3992039	3991986	ID=MS.gene28659.t1.exon2;Parent=MS.gene28659.t1
chr7.1	CDS	3991986	3992039	3991986	ID=cds.MS.gene28659.t1;Parent=MS.gene28659.t1
chr7.1	exon	3991438	3991457	3991438	ID=MS.gene28659.t1.exon3;Parent=MS.gene28659.t1
chr7.1	CDS	3991438	3991457	3991438	ID=cds.MS.gene28659.t1;Parent=MS.gene28659.t1

Gene Sequence

>MS.gene28659

ATGGCAGAACTGAGTTTGGGAATTCTAATTGACATTGTTGATGAGGATTGGATGAGAGACATTCTTCCTCAAGATGGTATTTTTTTTTTTCTTCTTTAATGCATTTTGTTGAGAAAAAAGATATGCGCCGTCAGTGTATACTAATTTACACCAACGCCAACTACAAATAGATTATTCGGACAGTTATCTTTAAAAAACAGTTGCAAAAAAAAGGCTACATAGCAAAATGGTTAGTTCCAATTAGACCCAACAGCGTGCATAACCATTGTAAATGAAATAAGAAGTTGAATCATCTATTGAGAGTCTTAAGCAGTCTTACACATTCTGTTTATGTAGTCTTAAACATTTTGTTACACCCATCCCTGTCCTATATTGTATATTGATAAATTGTCCTGAACTGACGGTACATATTTGAAAATACGTGAATAAATGAACAAATATAACAAAGTATAGATGGTTGGTTTACTTGTGATCTACAAAGTACGAAGAGAATGTAAGTTTAGGGATGGATGAAATTGGTGATATTTTCTTTTAACTTGATAATTGATATCCACGTGGGAATGTAAATCTGATGTGTGCTGTTTTTACAGATCTTCCACTGCCTCCAACACTGGTTGTTCGGACAGATGATACTGAGGACTCAAGTATGTTCTTAATTCATGACTAAAGCAGTTACATTTGTGGATACCATCTTTCTTGAGCTTAGTTTATTAAAACATTGTTGTATTAAGCACACGTTGATTTGGTGATGGGAAGATGTATAGTTAACAATGAATGAAAAAGATGTATACACGAGAAAACCAATCAAAAAGTTGCAATTTAGTGATGTGCCTTTCAATGAAAACATGGTTGTTTAAAGAAAATGTGTTCTTGGACATGCAACTGCACTCTTACAATTTATGATGCACAATGCATTACTGACAGTGTGGCATGTAAACAACGGTCATAACTTAAGAAAATTGAAGTGATTGATTGTAACTATATATGTTGCCATGTTGGTGTCGGATACAGACACATGTTGGACACTGAACATGTTTTCAAACTTATATGTCGGGGCTACATAACTTATAATAGTGGATTGATTATTGTTCATGAGATGGGTCTTATGTTGGAGGATTGTTTCTGCTTTTTGTTGTTGACTTTTTAACACCATGTGATTTGTTGTTAATCAGATCAGGAGCACAACAAGTAA

Protein sequence

>MS.gene28659.t1

MAELSLGILIDIVDEDWMRDILPQDDLPLPPTLVVRTDDTEDSNQEHNK