Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33634.t1 | RHN77890.1 | 93.4 | 211 | 13 | 1 | 1 | 211 | 46 | 255 | 7.00E-109 | 403.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33634.t1 | O04086 | 40.6 | 217 | 121 | 4 | 1 | 210 | 1 | 216 | 1.0e-36 | 154.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33634.t1 | A0A072VGE1 | 93.4 | 211 | 13 | 1 | 1 | 211 | 1 | 210 | 5.0e-109 | 403.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene33634 | -0.807084 | 6.01E-50 | -1.69E-46 |
| MS.gene049320 | MS.gene33634 | -0.80921 | 2.12E-50 | -1.69E-46 |
| MS.gene049633 | MS.gene33634 | -0.816477 | 5.42E-52 | -1.69E-46 |
| MS.gene050170 | MS.gene33634 | -0.804355 | 2.25E-49 | -1.69E-46 |
| MS.gene050381 | MS.gene33634 | 0.802448 | 5.58E-49 | -1.69E-46 |
| MS.gene050504 | MS.gene33634 | -0.805492 | 1.30E-49 | -1.69E-46 |
| MS.gene050612 | MS.gene33634 | -0.828209 | 1.02E-54 | -1.69E-46 |
| MS.gene050809 | MS.gene33634 | 0.822306 | 2.54E-53 | -1.69E-46 |
| MS.gene05080 | MS.gene33634 | -0.823686 | 1.21E-53 | -1.69E-46 |
| MS.gene050868 | MS.gene33634 | -0.811558 | 6.59E-51 | -1.69E-46 |
| MS.gene050871 | MS.gene33634 | -0.809828 | 1.56E-50 | -1.69E-46 |
| MS.gene051044 | MS.gene33634 | 0.816086 | 6.63E-52 | -1.69E-46 |
| MS.gene051127 | MS.gene33634 | 0.8062 | 9.23E-50 | -1.69E-46 |
| MS.gene051138 | MS.gene33634 | 0.851306 | 9.67E-61 | -1.69E-46 |
| MS.gene051364 | MS.gene33634 | 0.808169 | 3.53E-50 | -1.69E-46 |
| MS.gene051366 | MS.gene33634 | 0.807882 | 4.07E-50 | -1.69E-46 |
| MS.gene051651 | MS.gene33634 | 0.800496 | 1.40E-48 | -1.69E-46 |
| MS.gene052211 | MS.gene33634 | 0.809493 | 1.84E-50 | -1.69E-46 |
| MS.gene05240 | MS.gene33634 | 0.817375 | 3.41E-52 | -1.69E-46 |
| MS.gene052606 | MS.gene33634 | 0.809793 | 1.59E-50 | -1.69E-46 |
| MS.gene053420 | MS.gene33634 | 0.815331 | 9.76E-52 | -1.69E-46 |
| MS.gene053488 | MS.gene33634 | 0.823724 | 1.19E-53 | -1.69E-46 |
| MS.gene053561 | MS.gene33634 | 0.818253 | 2.16E-52 | -1.69E-46 |
| MS.gene05470 | MS.gene33634 | -0.801904 | 7.22E-49 | -1.69E-46 |
| MS.gene055124 | MS.gene33634 | 0.812933 | 3.30E-51 | -1.69E-46 |
| MS.gene055154 | MS.gene33634 | 0.819089 | 1.39E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene33634 | -0.801338 | 9.44E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene33634 | -0.811943 | 5.44E-51 | -1.69E-46 |
| MS.gene055577 | MS.gene33634 | -0.815472 | 9.08E-52 | -1.69E-46 |
| MS.gene055748 | MS.gene33634 | -0.812341 | 4.45E-51 | -1.69E-46 |
| MS.gene055751 | MS.gene33634 | -0.817476 | 3.23E-52 | -1.69E-46 |
| MS.gene05589 | MS.gene33634 | -0.834904 | 2.29E-56 | -1.69E-46 |
| MS.gene055942 | MS.gene33634 | 0.816492 | 5.38E-52 | -1.69E-46 |
| MS.gene056224 | MS.gene33634 | 0.803531 | 3.33E-49 | -1.69E-46 |
| MS.gene056359 | MS.gene33634 | 0.80643 | 8.26E-50 | -1.69E-46 |
| MS.gene056504 | MS.gene33634 | -0.815732 | 7.95E-52 | -1.69E-46 |
| MS.gene056590 | MS.gene33634 | 0.81477 | 1.30E-51 | -1.69E-46 |
| MS.gene056591 | MS.gene33634 | 0.813985 | 1.94E-51 | -1.69E-46 |
| MS.gene056748 | MS.gene33634 | 0.814141 | 1.79E-51 | -1.69E-46 |
| MS.gene056856 | MS.gene33634 | 0.850516 | 1.61E-60 | -1.69E-46 |
| MS.gene057768 | MS.gene33634 | 0.819295 | 1.25E-52 | -1.69E-46 |
| MS.gene058099 | MS.gene33634 | 0.805788 | 1.13E-49 | -1.69E-46 |
| MS.gene058109 | MS.gene33634 | 0.807925 | 3.98E-50 | -1.69E-46 |
| MS.gene058171 | MS.gene33634 | 0.814501 | 1.49E-51 | -1.69E-46 |
| MS.gene058173 | MS.gene33634 | 0.80674 | 7.10E-50 | -1.69E-46 |
| MS.gene058175 | MS.gene33634 | 0.817434 | 3.31E-52 | -1.69E-46 |
| MS.gene058334 | MS.gene33634 | 0.811801 | 5.84E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene33634 | -0.826268 | 2.98E-54 | -1.69E-46 |
| MS.gene058419 | MS.gene33634 | -0.806614 | 7.55E-50 | -1.69E-46 |
| MS.gene058603 | MS.gene33634 | 0.816441 | 5.52E-52 | -1.69E-46 |
| MS.gene058838 | MS.gene33634 | -0.801269 | 9.75E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene33634 | -0.81344 | 2.56E-51 | -1.69E-46 |
| MS.gene059023 | MS.gene33634 | -0.801055 | 1.08E-48 | -1.69E-46 |
| MS.gene059677 | MS.gene33634 | -0.802518 | 5.40E-49 | -1.69E-46 |
| MS.gene059691 | MS.gene33634 | 0.806442 | 8.21E-50 | -1.69E-46 |
| MS.gene059694 | MS.gene33634 | 0.804444 | 2.15E-49 | -1.69E-46 |
| MS.gene059697 | MS.gene33634 | 0.812672 | 3.77E-51 | -1.69E-46 |
| MS.gene059865 | MS.gene33634 | -0.826507 | 2.61E-54 | -1.69E-46 |
| MS.gene060762 | MS.gene33634 | -0.825085 | 5.68E-54 | -1.69E-46 |
| MS.gene06077 | MS.gene33634 | -0.808832 | 2.55E-50 | -1.69E-46 |
| MS.gene060982 | MS.gene33634 | -0.811905 | 5.54E-51 | -1.69E-46 |
| MS.gene061044 | MS.gene33634 | 0.829533 | 4.88E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33634.t1 | MTR_1g029600 | 93.365 | 211 | 13 | 1 | 1 | 211 | 1 | 210 | 4.27e-144 | 400 |
| MS.gene33634.t1 | MTR_1g029610 | 75.598 | 209 | 51 | 0 | 1 | 209 | 1 | 209 | 2.17e-116 | 344 |
| MS.gene33634.t1 | MTR_8g088780 | 45.536 | 224 | 102 | 5 | 4 | 209 | 2 | 223 | 5.58e-54 | 182 |
| MS.gene33634.t1 | MTR_2g006910 | 44.712 | 208 | 106 | 4 | 9 | 209 | 12 | 217 | 9.14e-50 | 171 |
| MS.gene33634.t1 | MTR_2g006910 | 44.712 | 208 | 106 | 4 | 9 | 209 | 12 | 217 | 9.98e-50 | 171 |
| MS.gene33634.t1 | MTR_8g088760 | 37.000 | 200 | 113 | 4 | 18 | 205 | 17 | 215 | 8.81e-37 | 135 |
| MS.gene33634.t1 | MTR_4g076660 | 42.969 | 128 | 70 | 2 | 86 | 211 | 1 | 127 | 8.76e-30 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene33634.t1 | AT1G11050 | 42.708 | 192 | 103 | 4 | 25 | 210 | 26 | 216 | 1.27e-37 | 138 |
Find 33 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATCTCTTCTTAGTCTCTT+TGG | 0.194083 | 1.1:+15204981 | MS.gene33634:CDS |
| GAAAGAGTGAAGTTTCTTTC+AGG | 0.244676 | 1.1:-15205022 | None:intergenic |
| ATTGGTATTGTGAATCATTT+TGG | 0.245591 | 1.1:+15205380 | MS.gene33634:CDS |
| CTTTACAAGAAGTGTCTAAA+AGG | 0.323911 | 1.1:-15205232 | None:intergenic |
| CAGAAGGAGTAGCTATGATA+AGG | 0.380662 | 1.1:-15204860 | None:intergenic |
| TTGTAAAGGTGATTTGAAAT+CGG | 0.381407 | 1.1:+15205247 | MS.gene33634:CDS |
| TGTGTCAATTGGGTCTCTGC+TGG | 0.389764 | 1.1:-15204946 | None:intergenic |
| CAACAATGTTGTGTGTCAAT+TGG | 0.393976 | 1.1:-15204957 | None:intergenic |
| CTTCTTAGTCTCTTTGGCAT+AGG | 0.437265 | 1.1:+15204987 | MS.gene33634:CDS |
| TAAGGGTGAAGAGGAGGAGA+AGG | 0.460180 | 1.1:-15204842 | None:intergenic |
| AACAATGTTGTGTGTCAATT+GGG | 0.477153 | 1.1:-15204956 | None:intergenic |
| TGCAGCATGTGCACTGATGC+TGG | 0.480741 | 1.1:+15205278 | MS.gene33634:CDS |
| GTATCACAAATATCAAAGAT+TGG | 0.483732 | 1.1:+15205186 | MS.gene33634:CDS |
| CTATGATAAGGGTGAAGAGG+AGG | 0.492847 | 1.1:-15204848 | None:intergenic |
| CGACAAATTGTGTTGAGTTG+TGG | 0.499718 | 1.1:-15205142 | None:intergenic |
| TAGCTATGATAAGGGTGAAG+AGG | 0.511716 | 1.1:-15204851 | None:intergenic |
| AGAAGGAGTAGCTATGATAA+GGG | 0.514017 | 1.1:-15204859 | None:intergenic |
| GCTGCAGCTAGTTGTATACT+TGG | 0.518609 | 1.1:+15205416 | MS.gene33634:CDS |
| CTGCTGGTTGATGTGTCCCA+TGG | 0.518922 | 1.1:-15204930 | None:intergenic |
| TTCTGCAGTATCATGTCCTA+TGG | 0.534711 | 1.1:+15204884 | MS.gene33634:CDS |
| ACGAAGGGACTAACGAAGGT+TGG | 0.536160 | 1.1:-15205115 | None:intergenic |
| GCAAACAACTCAAGCTGCGC+AGG | 0.537549 | 1.1:+15205164 | MS.gene33634:CDS |
| TCAACATAGCTGAGATCCAT+AGG | 0.544235 | 1.1:-15204900 | None:intergenic |
| CTAGCTGCAGCAGGATCTGT+AGG | 0.557782 | 1.1:-15205404 | None:intergenic |
| GGTTGATGTGTCCCATGGAA+AGG | 0.563360 | 1.1:-15204925 | None:intergenic |
| AAAATGATTCACAATACCAA+TGG | 0.590923 | 1.1:-15205378 | None:intergenic |
| AGTATACAACTAGCTGCAGC+AGG | 0.596113 | 1.1:-15205413 | None:intergenic |
| CTATGTTGAGACCTTTCCAT+GGG | 0.598491 | 1.1:+15204914 | MS.gene33634:CDS |
| CATGATACTGCAGAAGCAGA+AGG | 0.601540 | 1.1:-15204876 | None:intergenic |
| GCTATGTTGAGACCTTTCCA+TGG | 0.614762 | 1.1:+15204913 | MS.gene33634:CDS |
| TTGAGTTGTGGAAACACGAA+GGG | 0.701321 | 1.1:-15205130 | None:intergenic |
| GTTGAGTTGTGGAAACACGA+AGG | 0.744002 | 1.1:-15205131 | None:intergenic |
| AAACACGAAGGGACTAACGA+AGG | 0.755553 | 1.1:-15205119 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTAAAGTGTTTTTATTA+GGG | - | chr1.1:15205052-15205071 | None:intergenic | 10.0% |
| !!! | GATTTTAAAGTGTTTTTATT+AGG | - | chr1.1:15205053-15205072 | None:intergenic | 15.0% |
| ! | AAAATGATTCACAATACCAA+TGG | - | chr1.1:15205381-15205400 | None:intergenic | 25.0% |
| ! | AAAGATTGGAAGAAAAAAGT+TGG | + | chr1.1:15205200-15205219 | MS.gene33634:CDS | 25.0% |
| ! | GTATCACAAATATCAAAGAT+TGG | + | chr1.1:15205186-15205205 | MS.gene33634:CDS | 25.0% |
| !! | ATTGGTATTGTGAATCATTT+TGG | + | chr1.1:15205380-15205399 | MS.gene33634:CDS | 25.0% |
| !! | TTGTAAAGGTGATTTGAAAT+CGG | + | chr1.1:15205247-15205266 | MS.gene33634:CDS | 25.0% |
| !!! | TTTTCAGTTTTATATGCCAT+TGG | + | chr1.1:15205362-15205381 | MS.gene33634:CDS | 25.0% |
| AACAATGTTGTGTGTCAATT+GGG | - | chr1.1:15204959-15204978 | None:intergenic | 30.0% | |
| ! | CTTTTAGACACTTCTTGTAA+AGG | + | chr1.1:15205233-15205252 | MS.gene33634:CDS | 30.0% |
| !! | CTTTACAAGAAGTGTCTAAA+AGG | - | chr1.1:15205235-15205254 | None:intergenic | 30.0% |
| !!! | AAGCACTTAGTTGCATTTTT+TGG | - | chr1.1:15205338-15205357 | None:intergenic | 30.0% |
| AGAAGGAGTAGCTATGATAA+GGG | - | chr1.1:15204862-15204881 | None:intergenic | 35.0% | |
| CAACAATGTTGTGTGTCAAT+TGG | - | chr1.1:15204960-15204979 | None:intergenic | 35.0% | |
| CAATCTCTTCTTAGTCTCTT+TGG | + | chr1.1:15204981-15205000 | MS.gene33634:CDS | 35.0% | |
| GAAAGAGTGAAGTTTCTTTC+AGG | - | chr1.1:15205025-15205044 | None:intergenic | 35.0% | |
| CAGAAGGAGTAGCTATGATA+AGG | - | chr1.1:15204863-15204882 | None:intergenic | 40.0% | |
| CTATGTTGAGACCTTTCCAT+GGG | + | chr1.1:15204914-15204933 | MS.gene33634:CDS | 40.0% | |
| CTTCTTAGTCTCTTTGGCAT+AGG | + | chr1.1:15204987-15205006 | MS.gene33634:CDS | 40.0% | |
| TAGCTATGATAAGGGTGAAG+AGG | - | chr1.1:15204854-15204873 | None:intergenic | 40.0% | |
| TCAACATAGCTGAGATCCAT+AGG | - | chr1.1:15204903-15204922 | None:intergenic | 40.0% | |
| TTCTGCAGTATCATGTCCTA+TGG | + | chr1.1:15204884-15204903 | MS.gene33634:CDS | 40.0% | |
| ! | CGACAAATTGTGTTGAGTTG+TGG | - | chr1.1:15205145-15205164 | None:intergenic | 40.0% |
| ! | TTGAGTTGTGGAAACACGAA+GGG | - | chr1.1:15205133-15205152 | None:intergenic | 40.0% |
| AAACACGAAGGGACTAACGA+AGG | - | chr1.1:15205122-15205141 | None:intergenic | 45.0% | |
| AGTATACAACTAGCTGCAGC+AGG | - | chr1.1:15205416-15205435 | None:intergenic | 45.0% | |
| CATGATACTGCAGAAGCAGA+AGG | - | chr1.1:15204879-15204898 | None:intergenic | 45.0% | |
| CTATGATAAGGGTGAAGAGG+AGG | - | chr1.1:15204851-15204870 | None:intergenic | 45.0% | |
| GCTATGTTGAGACCTTTCCA+TGG | + | chr1.1:15204913-15204932 | MS.gene33634:CDS | 45.0% | |
| GCTGCAGCTAGTTGTATACT+TGG | + | chr1.1:15205416-15205435 | MS.gene33634:CDS | 45.0% | |
| ! | GTTGAGTTGTGGAAACACGA+AGG | - | chr1.1:15205134-15205153 | None:intergenic | 45.0% |
| ACGAAGGGACTAACGAAGGT+TGG | - | chr1.1:15205118-15205137 | None:intergenic | 50.0% | |
| TAAGGGTGAAGAGGAGGAGA+AGG | - | chr1.1:15204845-15204864 | None:intergenic | 50.0% | |
| TGTGTCAATTGGGTCTCTGC+TGG | - | chr1.1:15204949-15204968 | None:intergenic | 50.0% | |
| ! | GGTTGATGTGTCCCATGGAA+AGG | - | chr1.1:15204928-15204947 | None:intergenic | 50.0% |
| CTAGCTGCAGCAGGATCTGT+AGG | - | chr1.1:15205407-15205426 | None:intergenic | 55.0% | |
| GCAAACAACTCAAGCTGCGC+AGG | + | chr1.1:15205164-15205183 | MS.gene33634:CDS | 55.0% | |
| ! | CTGCTGGTTGATGTGTCCCA+TGG | - | chr1.1:15204933-15204952 | None:intergenic | 55.0% |
| ! | TGCAGCATGTGCACTGATGC+TGG | + | chr1.1:15205278-15205297 | MS.gene33634:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 15204822 | 15205457 | 15204822 | ID=MS.gene33634 |
| chr1.1 | mRNA | 15204822 | 15205457 | 15204822 | ID=MS.gene33634.t1;Parent=MS.gene33634 |
| chr1.1 | exon | 15204822 | 15205457 | 15204822 | ID=MS.gene33634.t1.exon1;Parent=MS.gene33634.t1 |
| chr1.1 | CDS | 15204822 | 15205457 | 15204822 | ID=cds.MS.gene33634.t1;Parent=MS.gene33634.t1 |
| Gene Sequence |
| Protein sequence |
