AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34233


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34233.t1 MTR_8g058785 98.450 129 2 0 1 129 1 129 6.38e-92 261
MS.gene34233.t1 MTR_8g061270 98.450 129 2 0 1 129 1 129 6.38e-92 261
MS.gene34233.t1 MTR_2g078670 71.094 128 37 0 1 128 1 128 6.86e-64 191
MS.gene34233.t1 MTR_4g088505 57.031 128 53 1 1 128 1 126 2.01e-48 151
MS.gene34233.t1 MTR_3g070330 52.344 128 61 0 1 128 24 151 8.08e-46 145
MS.gene34233.t1 MTR_3g070330 60.194 103 41 0 1 103 24 126 6.63e-45 142
MS.gene34233.t1 MTR_7g068250 57.273 110 47 0 1 110 1 110 2.45e-44 141
MS.gene34233.t1 MTR_5g082490 59.091 110 45 0 1 110 1 110 6.26e-44 140
MS.gene34233.t1 MTR_4g088505 61.446 83 32 0 1 83 1 83 1.45e-33 114
MS.gene34233.t1 MTR_3g091840 47.423 97 51 0 12 108 8 104 2.54e-31 107
MS.gene34233.t1 MTR_8g027255 42.857 98 56 0 9 106 5 102 2.59e-26 95.1
MS.gene34233.t1 MTR_1g077750 44.048 84 47 0 12 95 8 91 7.00e-26 94.0
MS.gene34233.t1 MTR_1g077760 42.593 54 31 0 48 101 1 54 4.98e-13 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34233.t1 AT5G53940 69.531 128 39 0 1 128 1 128 9.77e-62 185
MS.gene34233.t1 AT2G40110 55.814 129 56 1 1 128 1 129 4.28e-47 148
MS.gene34233.t1 AT3G08990 51.562 128 62 0 1 128 1 128 1.43e-46 147
MS.gene34233.t1 AT3G08990 51.562 128 62 0 1 128 1 128 1.43e-46 147
MS.gene34233.t1 AT3G08990 51.562 128 62 0 1 128 1 128 1.43e-46 147
MS.gene34233.t1 AT2G40110 64.706 102 36 0 1 102 1 102 1.57e-46 146
MS.gene34233.t1 AT3G55890 58.095 105 44 0 1 105 1 105 7.29e-42 135
MS.gene34233.t1 AT3G55890 58.095 105 44 0 1 105 1 105 7.29e-42 135
MS.gene34233.t1 AT3G55890 59.406 101 41 0 1 101 1 101 2.34e-41 133
MS.gene34233.t1 AT3G55890 59.406 101 41 0 1 101 1 101 2.34e-41 133
MS.gene34233.t1 AT3G11230 50.781 128 63 0 1 128 1 128 4.74e-41 133
MS.gene34233.t1 AT3G11230 50.781 128 63 0 1 128 34 161 1.82e-40 132
MS.gene34233.t1 AT3G11230 57.843 102 43 0 1 102 1 102 1.56e-39 128
MS.gene34233.t1 AT3G11230 57.843 102 43 0 1 102 34 135 2.86e-39 129
MS.gene34233.t1 AT2G40110 61.446 83 32 0 1 83 1 83 1.99e-35 117
MS.gene34233.t1 AT2G40110 61.446 83 32 0 1 83 1 83 1.99e-35 117
MS.gene34233.t1 AT4G27745 45.192 104 57 0 5 108 1 104 5.72e-31 107
MS.gene34233.t1 AT4G27745 45.192 104 57 0 5 108 1 104 5.72e-31 107
MS.gene34233.t1 AT4G27745 45.192 104 57 0 5 108 1 104 5.72e-31 107
MS.gene34233.t1 AT4G27745 45.192 104 57 0 5 108 1 104 5.72e-31 107
MS.gene34233.t1 AT4G27740 39.130 92 56 0 14 105 10 101 6.54e-23 86.3

Find 22 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGGAAAGAGCGATGCTTTC+AGG 0.190195 8.4:-47557842 MS.gene34233:CDS
ACTTTCCCTCTTTGTACTTT+TGG 0.194241 8.4:+47557607 None:intergenic
TCCACTGAATTCTGTGTTGA+AGG 0.319590 8.4:-47557444 MS.gene34233:CDS
GAGCTGGATGGCAGAGTTTA+CGG 0.331875 8.4:-47558666 MS.gene34233:CDS
TGTCGCTGCGGTCAGATAAT+TGG 0.336845 8.4:-47557791 MS.gene34233:CDS
GAGTGATACTGAAGATGATT+AGG 0.429995 8.4:-47557409 None:intergenic
GGAAGGATCGTTGATGAAAT+CGG 0.431542 8.4:-47557471 MS.gene34233:CDS
ATACTTACACATTATTGAAA+AGG 0.455887 8.4:+47558461 None:intergenic
TTTCCACTGATCTCATCTCC+AGG 0.460967 8.4:-47558614 MS.gene34233:CDS
GAAAAGCCAAAAGTACAAAG+AGG 0.465104 8.4:-47557613 MS.gene34233:CDS
AGGGAAAGTTTGTTCTTGAA+AGG 0.474035 8.4:-47557593 MS.gene34233:intron
TGGAAAGAGCGATGCTTTCA+GGG 0.476055 8.4:-47557841 MS.gene34233:CDS
AAAAGCCAAAAGTACAAAGA+GGG 0.574439 8.4:-47557612 MS.gene34233:CDS
TTCCACTGATCTCATCTCCA+GGG 0.580481 8.4:-47558613 MS.gene34233:intron
GCTGCGGTCAGATAATTGGC+TGG 0.590004 8.4:-47557787 MS.gene34233:CDS
CACAGTGTGAACTTCACTAG+TGG 0.591434 8.4:-47557872 MS.gene34233:intron
CTTCACTAGTGGAGAAGTGG+TGG 0.595583 8.4:-47557861 MS.gene34233:CDS
TCTCTCATAGGAAACTGTGC+AGG 0.598241 8.4:-47557637 MS.gene34233:intron
TGAGATCAGTGGAAAGAGCA+AGG 0.604806 8.4:+47558622 None:intergenic
GCCTTCAACACAGAATTCAG+TGG 0.633670 8.4:+47557443 None:intergenic
TACCCTGGAGATGAGATCAG+TGG 0.654916 8.4:+47558611 None:intergenic
GAACTTCACTAGTGGAGAAG+TGG 0.657506 8.4:-47557864 MS.gene34233:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATACAATCTAATTCTAATAT+CGG + chr8.4:47558120-47558139 None:intergenic 15.0%
!!! TTCAATTTTTTTGGTTAATT+AGG - chr8.4:47557531-47557550 MS.gene34233:intron 15.0%
!! ATACTTACACATTATTGAAA+AGG + chr8.4:47557639-47557658 None:intergenic 20.0%
!! TAGATTTGTAAAAATATGAG+AGG + chr8.4:47558358-47558377 None:intergenic 20.0%
!! TCAATAATGGTATTATAGTA+AGG - chr8.4:47557739-47557758 MS.gene34233:intron 20.0%
!! TGTATGTTAAGTTATATACT+GGG - chr8.4:47557915-47557934 MS.gene34233:intron 20.0%
!! TTAACCAAAAAAATTGAAGA+AGG + chr8.4:47557529-47557548 None:intergenic 20.0%
!! TTGTATGTTAAGTTATATAC+TGG - chr8.4:47557914-47557933 MS.gene34233:intron 20.0%
!!! AATGCAATTTTATTTTCAAC+TGG + chr8.4:47557795-47557814 None:intergenic 20.0%
!!! GACAGTTTTATTATCAATAA+TGG - chr8.4:47557726-47557745 MS.gene34233:intron 20.0%
! AGAAGAAAAGCAAACAATAA+GGG + chr8.4:47557584-47557603 None:intergenic 25.0%
! AGATAAGTATTTCAACATTC+AGG + chr8.4:47558041-47558060 None:intergenic 25.0%
! ATCAGTAAAAACACACATTA+AGG + chr8.4:47558576-47558595 None:intergenic 25.0%
! GATAAGTATTTCAACATTCA+GGG + chr8.4:47558040-47558059 None:intergenic 25.0%
! TTTGTAAAAATATGAGAGGA+TGG + chr8.4:47558354-47558373 None:intergenic 25.0%
!!! CATTTAAGATTTGTTACTGA+TGG + chr8.4:47557689-47557708 None:intergenic 25.0%
!!! TGATCCTTCTTCAATTTTTT+TGG - chr8.4:47557522-47557541 MS.gene34233:intron 25.0%
AAACAATAAGGGTTAACGAA+TGG + chr8.4:47557573-47557592 None:intergenic 30.0%
GAGAAGAAAAGCAAACAATA+AGG + chr8.4:47557585-47557604 None:intergenic 30.0%
TAAAATTGCATCTCTCTCAT+AGG - chr8.4:47558448-47558467 MS.gene34233:intron 30.0%
! AAAAGCCAAAAGTACAAAGA+GGG - chr8.4:47558485-47558504 MS.gene34233:CDS 30.0%
! TTTAGAAGGATCAAAAGTAC+TGG + chr8.4:47558432-47558451 None:intergenic 30.0%
!! ATTGTTTGCTTTTCTTCTCA+GGG - chr8.4:47557586-47557605 MS.gene34233:intron 30.0%
!! TATTGTTTGCTTTTCTTCTC+AGG - chr8.4:47557585-47557604 MS.gene34233:intron 30.0%
CATGATATGATATGATACCC+TGG + chr8.4:47557504-47557523 None:intergenic 35.0%
CTCAACAAATTGAAATGCCT+CGG + chr8.4:47557881-47557900 None:intergenic 35.0%
GTAAAAATATGAGAGGATGG+TGG + chr8.4:47558351-47558370 None:intergenic 35.0%
TATACTGGGATTGTTGTAAC+TGG - chr8.4:47557929-47557948 MS.gene34233:intron 35.0%
TATCTGCAAACTAGTGTTGT+AGG - chr8.4:47558378-47558397 MS.gene34233:intron 35.0%
! ACTTTCCCTCTTTGTACTTT+TGG + chr8.4:47558493-47558512 None:intergenic 35.0%
! AGGGAAAGTTTGTTCTTGAA+AGG - chr8.4:47558504-47558523 MS.gene34233:intron 35.0%
! AGTTTTCATGGTTGAATGGT+GGG - chr8.4:47557962-47557981 MS.gene34233:intron 35.0%
! CAGGGATTTTATTGTCATAG+AGG - chr8.4:47557604-47557623 MS.gene34233:CDS 35.0%
! GAAAAGCCAAAAGTACAAAG+AGG - chr8.4:47558484-47558503 MS.gene34233:CDS 35.0%
! GAGAGAGATGCAATTTTAGA+AGG + chr8.4:47558446-47558465 None:intergenic 35.0%
! TGTCAGTTTTCATGGTTGAA+TGG - chr8.4:47557958-47557977 MS.gene34233:intron 35.0%
!! GATATATTTTGTTGTCGCTG+CGG - chr8.4:47558294-47558313 MS.gene34233:intron 35.0%
ACAAAACTCCCACATACGTA+AGG + chr8.4:47558001-47558020 None:intergenic 40.0%
ACGATCCTTCCTCTGTTAAA+TGG + chr8.4:47558617-47558636 None:intergenic 40.0%
CATGCTTGTCCATTTAACAG+AGG - chr8.4:47558605-47558624 MS.gene34233:intron 40.0%
CTAATACCAAACTAATGCCG+AGG - chr8.4:47557861-47557880 MS.gene34233:CDS 40.0%
CTTGTCCATTTAACAGAGGA+AGG - chr8.4:47558609-47558628 MS.gene34233:intron 40.0%
TCCACTGAATTCTGTGTTGA+AGG - chr8.4:47558653-47558672 MS.gene34233:CDS 40.0%
! ATTTTTGCAGTTGAGCTGGA+TGG - chr8.4:47557419-47557438 MS.gene34233:CDS 40.0%
! CAGTTTTCATGGTTGAATGG+TGG - chr8.4:47557961-47557980 MS.gene34233:intron 40.0%
! CATGTGCATGCTTGTGTTTT+TGG - chr8.4:47558195-47558214 MS.gene34233:intron 40.0%
! GAGAATTTTTGCAGTTGAGC+TGG - chr8.4:47557415-47557434 MS.gene34233:CDS 40.0%
! GGAAGGATCGTTGATGAAAT+CGG - chr8.4:47558626-47558645 MS.gene34233:CDS 40.0%
!! TATGTGGGAGTTTTGTACAC+CGG - chr8.4:47558005-47558024 MS.gene34233:intron 40.0%
ACATACGTAAGGTCCTGGAA+AGG + chr8.4:47557990-47558009 None:intergenic 45.0%
CACAGTGTGAACTTCACTAG+TGG - chr8.4:47558225-47558244 MS.gene34233:intron 45.0%
CATACGTAAGGTCCTGGAAA+GGG + chr8.4:47557989-47558008 None:intergenic 45.0%
GAAATGCCTCGGCATTAGTT+TGG + chr8.4:47557870-47557889 None:intergenic 45.0%
GAACTTCACTAGTGGAGAAG+TGG - chr8.4:47558233-47558252 MS.gene34233:intron 45.0%
GCCTTCAACACAGAATTCAG+TGG + chr8.4:47558657-47558676 None:intergenic 45.0%
GTGAAGTTCACACTGTGTCA+TGG + chr8.4:47558222-47558241 None:intergenic 45.0%
TCTCTCATAGGAAACTGTGC+AGG - chr8.4:47558460-47558479 MS.gene34233:intron 45.0%
TGAGATCAGTGGAAAGAGCA+AGG + chr8.4:47557478-47557497 None:intergenic 45.0%
TGGAAAGAGCGATGCTTTCA+GGG - chr8.4:47558256-47558275 MS.gene34233:intron 45.0%
TTCCACTGATCTCATCTCCA+GGG - chr8.4:47557484-47557503 MS.gene34233:intron 45.0%
TTCCAGGACCTTACGTATGT+GGG - chr8.4:47557990-47558009 MS.gene34233:intron 45.0%
TTTCCACTGATCTCATCTCC+AGG - chr8.4:47557483-47557502 MS.gene34233:intron 45.0%
TTTCCAGGACCTTACGTATG+TGG - chr8.4:47557989-47558008 MS.gene34233:intron 45.0%
! AAGAGCAAGGTGAGTTTTGC+AGG + chr8.4:47557465-47557484 None:intergenic 45.0%
! ATTCTGCGTCTGTGTTGATG+TGG - chr8.4:47557812-47557831 MS.gene34233:CDS 45.0%
!!! GGCTAGCTTGTCAGTTTTCA+TGG - chr8.4:47557950-47557969 MS.gene34233:intron 45.0%
CTTCACTAGTGGAGAAGTGG+TGG - chr8.4:47558236-47558255 MS.gene34233:intron 50.0%
GAGCTGGATGGCAGAGTTTA+CGG - chr8.4:47557431-47557450 MS.gene34233:CDS 50.0%
GTGGAAAGAGCGATGCTTTC+AGG - chr8.4:47558255-47558274 MS.gene34233:intron 50.0%
TACCCTGGAGATGAGATCAG+TGG + chr8.4:47557489-47557508 None:intergenic 50.0%
TGTCGCTGCGGTCAGATAAT+TGG - chr8.4:47558306-47558325 MS.gene34233:intron 50.0%
!! TGGGAGTTTTGTACACCGGA+CGG - chr8.4:47558009-47558028 MS.gene34233:intron 50.0%
CTCCCACATACGTAAGGTCC+TGG + chr8.4:47557995-47558014 None:intergenic 55.0%
GCTGCGGTCAGATAATTGGC+TGG - chr8.4:47558310-47558329 MS.gene34233:intron 55.0%
! TGAATGGTGGGACCCTTTCC+AGG - chr8.4:47557974-47557993 MS.gene34233:intron 55.0%
!! GGGAGTTTTGTACACCGGAC+GGG - chr8.4:47558010-47558029 MS.gene34233:intron 55.0%
ACATTCAGGGCAGTCCCGTC+CGG + chr8.4:47558027-47558046 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 47557410 47558709 47557410 ID=MS.gene34233
chr8.4 mRNA 47557410 47558709 47557410 ID=MS.gene34233.t1;Parent=MS.gene34233
chr8.4 exon 47558614 47558709 47558614 ID=MS.gene34233.t1.exon1;Parent=MS.gene34233.t1
chr8.4 CDS 47558614 47558709 47558614 ID=cds.MS.gene34233.t1;Parent=MS.gene34233.t1
chr8.4 exon 47558469 47558512 47558469 ID=MS.gene34233.t1.exon2;Parent=MS.gene34233.t1
chr8.4 CDS 47558469 47558512 47558469 ID=cds.MS.gene34233.t1;Parent=MS.gene34233.t1
chr8.4 exon 47557781 47557889 47557781 ID=MS.gene34233.t1.exon3;Parent=MS.gene34233.t1
chr8.4 CDS 47557781 47557889 47557781 ID=cds.MS.gene34233.t1;Parent=MS.gene34233.t1
chr8.4 exon 47557594 47557649 47557594 ID=MS.gene34233.t1.exon4;Parent=MS.gene34233.t1
chr8.4 CDS 47557594 47557649 47557594 ID=cds.MS.gene34233.t1;Parent=MS.gene34233.t1
chr8.4 exon 47557410 47557494 47557410 ID=MS.gene34233.t1.exon5;Parent=MS.gene34233.t1
chr8.4 CDS 47557410 47557494 47557410 ID=cds.MS.gene34233.t1;Parent=MS.gene34233.t1
Gene Sequence

>MS.gene34233

ATGGGGAGAATTTTTGCAGTTGAGCTGGATGGCAGAGTTTACGGCTGCAAGTCCTGCAAAACTCACCTTGCTCTTTCCACTGATCTCATCTCCAGGGTATCATATCATATCATGATCCTTCTTCAATTTTTTTGGTTAATTAGGTGTTTCAACTAATTAACCATTCGTTAACCCTTATTGTTTGCTTTTCTTCTCAGGGATTTTATTGTCATAGAGGAAAAGCATACCTTTTCAATAATGTGTAAGTATTTTTCATTTTTTGTTTTCTTTGTTAATCCATCAGTAACAAATCTTAAATGAATTACTACAAAGTTATGACAGTTTTATTATCAATAATGGTATTATAGTAAGGCTTATATCATTTATTATTGTTCTCACTTTTCCAGTTGAAAATAAAATTGCATTCTGCGTCTGTGTTGATGTGGAAAATGTATTAGTGTGTTTCTAATTTCTAATACCAAACTAATGCCGAGGCATTTCAATTTGTTGAGCTACATATAAGAATTGTATGTTAAGTTATATACTGGGATTGTTGTAACTGGCTAGCTTGTCAGTTTTCATGGTTGAATGGTGGGACCCTTTCCAGGACCTTACGTATGTGGGAGTTTTGTACACCGGACGGGACTGCCCTGAATGTTGAAATACTTATCTTATGCTTTGATTAGATTATTATGTATAATTGTACATGCTTCAACATCTTTGACATACCGATATTAGAATTAGATTGTATTGATTGAAATTATAAATACAAACTAGATCTATGCTTTTTTATTATTAACAATTCTCATGTGCATGCTTGTGTTTTTGGTCCATGACACAGTGTGAACTTCACTAGTGGAGAAGTGGTGGAAAGAGCGATGCTTTCAGGGATGCATACTGTTGCAGATATATTTTGTTGTCGCTGCGGTCAGATAATTGGCTGGAAATACGTAATTCTTCCACCATCCTCTCATATTTTTACAAATCTATATCTGCAAACTAGTGTTGTAGGTGAAATCTATTTGAATATCATTCTGCATCCAGTACTTTTGATCCTTCTAAAATTGCATCTCTCTCATAGGAAACTGTGCAGGAGAAAAGCCAAAAGTACAAAGAGGGAAAGTTTGTTCTTGAAAGGTATTATGCTATTTTGCTATCACAACTTGAGTTGAGTTTTTTCTTATCCTTAATGTGTGTTTTTACTGATTGTTCATGTCATGCTTGTCCATTTAACAGAGGAAGGATCGTTGATGAAATCGGTTTCTCCACTGAATTCTGTGTTGAAGGCTGTGTCAGCATGAGTGATACTGAAGATGATTAG

Protein sequence

>MS.gene34233.t1

MGRIFAVELDGRVYGCKSCKTHLALSTDLISRGFYCHRGKAYLFNNVVNFTSGEVVERAMLSGMHTVADIFCCRCGQIIGWKYETVQEKSQKYKEGKFVLERGRIVDEIGFSTEFCVEGCVSMSDTEDD