AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38741


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38741.t1 MTR_3g101260 96.923 130 4 0 17 146 1 130 6.14e-92 262
MS.gene38741.t1 MTR_4g086730 46.610 118 63 0 29 146 61 178 2.94e-35 120
MS.gene38741.t1 MTR_4g086750 46.610 118 63 0 29 146 62 179 3.06e-35 120
MS.gene38741.t1 MTR_4g086740 46.610 118 63 0 29 146 60 177 3.09e-35 120
MS.gene38741.t1 MTR_4g086710 45.763 118 64 0 29 146 62 179 5.29e-35 120
MS.gene38741.t1 MTR_4g086690 42.529 87 50 0 60 146 21 107 5.54e-20 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38741.t1 AT2G15780 42.478 113 63 1 29 141 142 252 5.42e-25 96.3
MS.gene38741.t1 AT2G15770 38.793 116 69 1 26 141 141 254 1.31e-23 93.6
MS.gene38741.t1 AT4G33930 35.897 117 58 6 39 144 229 339 4.77e-12 62.4
MS.gene38741.t1 AT4G34300 35.652 115 56 6 39 141 198 306 2.08e-11 60.5

Find 47 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCCAATGTGTCATTTATT+TGG 0.160335 3.3:-83902666 None:intergenic
CTTCCTTGTGATTGCTTCAA+TGG 0.264782 3.3:+83902531 MS.gene38741:CDS
TCCAACCAAGATGCTTCTTT+TGG 0.296074 3.3:-83902581 None:intergenic
GTGATTGCTTCAATGGTTGC+AGG 0.311734 3.3:+83902538 MS.gene38741:CDS
GGGAATTGGACTAAATTAAA+AGG 0.331947 3.3:-83903163 None:intergenic
TCATCAGAGGCAAAGTAATA+AGG 0.345121 3.3:-83903078 None:intergenic
TACACGACGTGTGAATTTAG+AGG 0.385924 3.3:+83902985 MS.gene38741:CDS
GCTTCAATGGTTGCAGGGTC+AGG 0.401233 3.3:+83902544 MS.gene38741:CDS
GACTCTGAAGGTTGGCGTGC+AGG 0.411668 3.3:+83902604 MS.gene38741:CDS
AATTAAAAGGAACGTGTAGA+TGG 0.428002 3.3:-83903150 None:intergenic
TATTACTTTGCCTCTGATGA+AGG 0.434564 3.3:+83903081 MS.gene38741:CDS
AATGCCTATGACTGTATTGC+TGG 0.454610 3.3:+83903105 MS.gene38741:CDS
GAGTCCAGCAATACAGTCAT+AGG 0.464321 3.3:-83903109 None:intergenic
GTCGTGTAACTCCACATATT+CGG 0.471065 3.3:-83902970 None:intergenic
CAATGTGTCATTTATTTGGA+AGG 0.485349 3.3:-83902662 None:intergenic
TTTAGAGGAGCAAAGTTGCT+TGG 0.490765 3.3:+83903000 MS.gene38741:CDS
TTTACTTGCTACCGAATATG+TGG 0.498287 3.3:+83902959 MS.gene38741:CDS
TTCCAAATAAATGACACATT+GGG 0.508285 3.3:+83902664 MS.gene38741:CDS
ATGTGTCATTTATTTGGAAG+GGG 0.525400 3.3:-83902660 None:intergenic
AATGTGTCATTTATTTGGAA+GGG 0.530429 3.3:-83902661 None:intergenic
CTTCCAAATAAATGACACAT+TGG 0.530831 3.3:+83902663 MS.gene38741:CDS
CAGAACCAAAAGAAGCATCT+TGG 0.539687 3.3:+83902576 MS.gene38741:CDS
ACCAAAAGAAGCATCTTGGT+TGG 0.551375 3.3:+83902580 MS.gene38741:CDS
GGTTGCAGGGTCAGGAAGCA+AGG 0.552711 3.3:+83902552 MS.gene38741:CDS
CAGGCACTAACTACACCCAA+TGG 0.558303 3.3:+83902623 MS.gene38741:CDS
GCTGATCAAGGTGGTGGTGA+GGG 0.565763 3.3:+83903027 MS.gene38741:CDS
TGATTGCTTCAATGGTTGCA+GGG 0.566600 3.3:+83902539 MS.gene38741:CDS
TTCGGTAGCAAGTAAACGCT+GGG 0.568902 3.3:-83902952 None:intergenic
TGGTTGGAGACTCTGAAGGT+TGG 0.582794 3.3:+83902596 MS.gene38741:CDS
AGGCACTAACTACACCCAAT+GGG 0.584087 3.3:+83902624 MS.gene38741:CDS
ATTCGGTAGCAAGTAAACGC+TGG 0.587303 3.3:-83902953 None:intergenic
AACCGTGGAATTTCCTGAAG+GGG 0.598371 3.3:-83902926 None:intergenic
AGATGGAACAGCAATGAACT+TGG 0.599581 3.3:-83903133 None:intergenic
TAAAGATTGAGCTGAATCAG+TGG 0.605281 3.3:+83903052 MS.gene38741:CDS
TGCTGATCAAGGTGGTGGTG+AGG 0.607379 3.3:+83903026 MS.gene38741:CDS
ATAGGCATTGCCTTCATCAG+AGG 0.610448 3.3:-83903091 None:intergenic
ATGTCTAGAATTCTCATACA+AGG 0.613782 3.3:+83902502 MS.gene38741:CDS
GGTAATGCTGATCAAGGTGG+TGG 0.619304 3.3:+83903021 MS.gene38741:CDS
ACCGTGGAATTTCCTGAAGG+GGG 0.620984 3.3:-83902925 None:intergenic
TTGCTTGGTAATGCTGATCA+AGG 0.621103 3.3:+83903015 MS.gene38741:CDS
ATCTTGGTTGGAGACTCTGA+AGG 0.629130 3.3:+83902592 MS.gene38741:CDS
CAACCATTGAAGCAATCACA+AGG 0.631574 3.3:-83902534 None:intergenic
CAACCGTGGAATTTCCTGAA+GGG 0.654328 3.3:-83902927 None:intergenic
ACAACCGTGGAATTTCCTGA+AGG 0.668113 3.3:-83902928 None:intergenic
CTTGGTAATGCTGATCAAGG+TGG 0.670664 3.3:+83903018 MS.gene38741:CDS
GTAAACGCTGGGAACAACCG+TGG 0.691075 3.3:-83902941 None:intergenic
CCGTGGAATTTCCTGAAGGG+GGG 0.694717 3.3:-83902924 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTCAAATTATTATCAA+AGG + chr3.3:83902792-83902811 MS.gene38741:intron 10.0%
!! ATAATTTGAAAAAATCAAAG+TGG - chr3.3:83902786-83902805 None:intergenic 15.0%
!! TTAAATATAATTAAATGCGT+TGG - chr3.3:83902763-83902782 None:intergenic 15.0%
!!! TTTGATTGATGGATTAATTT+TGG + chr3.3:83902867-83902886 MS.gene38741:intron 20.0%
! CAATATATATGCACAAACAA+TGG - chr3.3:83902830-83902849 None:intergenic 25.0%
! TTCCAAATAAATGACACATT+GGG + chr3.3:83902664-83902683 MS.gene38741:CDS 25.0%
!!! AATGTGTCATTTATTTGGAA+GGG - chr3.3:83902664-83902683 None:intergenic 25.0%
CTTCCAAATAAATGACACAT+TGG + chr3.3:83902663-83902682 MS.gene38741:CDS 30.0%
CTTCTTCTTCATTTGATTGA+TGG + chr3.3:83902856-83902875 MS.gene38741:intron 30.0%
! ATGTCTAGAATTCTCATACA+AGG + chr3.3:83902502-83902521 MS.gene38741:CDS 30.0%
!! ATGTGTCATTTATTTGGAAG+GGG - chr3.3:83902663-83902682 None:intergenic 30.0%
!! CAATGTGTCATTTATTTGGA+AGG - chr3.3:83902665-83902684 None:intergenic 30.0%
!! TACCCAATGTGTCATTTATT+TGG - chr3.3:83902669-83902688 None:intergenic 30.0%
TAAAGATTGAGCTGAATCAG+TGG + chr3.3:83903052-83903071 MS.gene38741:CDS 35.0%
TATTACTTTGCCTCTGATGA+AGG + chr3.3:83903081-83903100 MS.gene38741:CDS 35.0%
TCATCAGAGGCAAAGTAATA+AGG - chr3.3:83903081-83903100 None:intergenic 35.0%
! TTTACTTGCTACCGAATATG+TGG + chr3.3:83902959-83902978 MS.gene38741:CDS 35.0%
!!! GCTATTTTTTATGGCCCATT+GGG - chr3.3:83902641-83902660 None:intergenic 35.0%
!!! TTGGAAGGGGCTATTTTTTA+TGG - chr3.3:83902650-83902669 None:intergenic 35.0%
AATGCCTATGACTGTATTGC+TGG + chr3.3:83903105-83903124 MS.gene38741:CDS 40.0%
AGATGGAACAGCAATGAACT+TGG - chr3.3:83903136-83903155 None:intergenic 40.0%
CAACCATTGAAGCAATCACA+AGG - chr3.3:83902537-83902556 None:intergenic 40.0%
CTTCCTTGTGATTGCTTCAA+TGG + chr3.3:83902531-83902550 MS.gene38741:CDS 40.0%
GTCGTGTAACTCCACATATT+CGG - chr3.3:83902973-83902992 None:intergenic 40.0%
TACACGACGTGTGAATTTAG+AGG + chr3.3:83902985-83903004 MS.gene38741:CDS 40.0%
TGATTGCTTCAATGGTTGCA+GGG + chr3.3:83902539-83902558 MS.gene38741:CDS 40.0%
TTTAGAGGAGCAAAGTTGCT+TGG + chr3.3:83903000-83903019 MS.gene38741:CDS 40.0%
! TCCAACCAAGATGCTTCTTT+TGG - chr3.3:83902584-83902603 None:intergenic 40.0%
!! ACCAAAAGAAGCATCTTGGT+TGG + chr3.3:83902580-83902599 MS.gene38741:CDS 40.0%
!! CAGAACCAAAAGAAGCATCT+TGG + chr3.3:83902576-83902595 MS.gene38741:CDS 40.0%
!! TTGCTTGGTAATGCTGATCA+AGG + chr3.3:83903015-83903034 MS.gene38741:CDS 40.0%
!!! CAAGATGCTTCTTTTGGTTC+TGG - chr3.3:83902578-83902597 None:intergenic 40.0%
!!! GGCTATTTTTTATGGCCCAT+TGG - chr3.3:83902642-83902661 None:intergenic 40.0%
AACCGTGGAATTTCCTGAAG+GGG - chr3.3:83902929-83902948 None:intergenic 45.0%
ACAACCGTGGAATTTCCTGA+AGG - chr3.3:83902931-83902950 None:intergenic 45.0%
AGGCACTAACTACACCCAAT+GGG + chr3.3:83902624-83902643 MS.gene38741:CDS 45.0%
ATCTTGGTTGGAGACTCTGA+AGG + chr3.3:83902592-83902611 MS.gene38741:CDS 45.0%
ATTCGGTAGCAAGTAAACGC+TGG - chr3.3:83902956-83902975 None:intergenic 45.0%
CAACCGTGGAATTTCCTGAA+GGG - chr3.3:83902930-83902949 None:intergenic 45.0%
GAGTCCAGCAATACAGTCAT+AGG - chr3.3:83903112-83903131 None:intergenic 45.0%
GTGATTGCTTCAATGGTTGC+AGG + chr3.3:83902538-83902557 MS.gene38741:CDS 45.0%
TTCGGTAGCAAGTAAACGCT+GGG - chr3.3:83902955-83902974 None:intergenic 45.0%
! ATAGGCATTGCCTTCATCAG+AGG - chr3.3:83903094-83903113 None:intergenic 45.0%
!! CTTGGTAATGCTGATCAAGG+TGG + chr3.3:83903018-83903037 MS.gene38741:CDS 45.0%
ACCGTGGAATTTCCTGAAGG+GGG - chr3.3:83902928-83902947 None:intergenic 50.0%
CAGGCACTAACTACACCCAA+TGG + chr3.3:83902623-83902642 MS.gene38741:CDS 50.0%
GGTAATGCTGATCAAGGTGG+TGG + chr3.3:83903021-83903040 MS.gene38741:CDS 50.0%
! TGGTTGGAGACTCTGAAGGT+TGG + chr3.3:83902596-83902615 MS.gene38741:CDS 50.0%
CCCCCCTTCAGGAAATTCCA+CGG + chr3.3:83902924-83902943 MS.gene38741:CDS 55.0%
CCGTGGAATTTCCTGAAGGG+GGG - chr3.3:83902927-83902946 None:intergenic 55.0%
GCTTCAATGGTTGCAGGGTC+AGG + chr3.3:83902544-83902563 MS.gene38741:CDS 55.0%
GTAAACGCTGGGAACAACCG+TGG - chr3.3:83902944-83902963 None:intergenic 55.0%
GTGTTCAAGTACCCCCCTTC+AGG + chr3.3:83902913-83902932 MS.gene38741:intron 55.0%
! GCTGATCAAGGTGGTGGTGA+GGG + chr3.3:83903027-83903046 MS.gene38741:CDS 55.0%
! TGCTGATCAAGGTGGTGGTG+AGG + chr3.3:83903026-83903045 MS.gene38741:CDS 55.0%
GGTTGCAGGGTCAGGAAGCA+AGG + chr3.3:83902552-83902571 MS.gene38741:CDS 60.0%
! GACTCTGAAGGTTGGCGTGC+AGG + chr3.3:83902604-83902623 MS.gene38741:CDS 60.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 83902502 83903170 83902502 ID=MS.gene38741
chr3.3 mRNA 83902502 83903170 83902502 ID=MS.gene38741.t1;Parent=MS.gene38741
chr3.3 exon 83902502 83902685 83902502 ID=MS.gene38741.t1.exon1;Parent=MS.gene38741.t1
chr3.3 CDS 83902502 83902685 83902502 ID=cds.MS.gene38741.t1;Parent=MS.gene38741.t1
chr3.3 exon 83902914 83903170 83902914 ID=MS.gene38741.t1.exon2;Parent=MS.gene38741.t1
chr3.3 CDS 83902914 83903170 83902914 ID=cds.MS.gene38741.t1;Parent=MS.gene38741.t1
Gene Sequence

>MS.gene38741

ATGTCTAGAATTCTCATACAAGGAGTTATCTTCCTTGTGATTGCTTCAATGGTTGCAGGGTCAGGAAGCAAGGCCAGAACCAAAAGAAGCATCTTGGTTGGAGACTCTGAAGGTTGGCGTGCAGGCACTAACTACACCCAATGGGCCATAAAAAATAGCCCCTTCCAAATAAATGACACATTGGGTATGTATCAATTAACTCGTAGCAGCGATACTACTTCATATCGAAAACATTTGTTTTTTGACACTAACACGAGACCAACGCATTTAATTATATTTAACCACTTTGATTTTTTCAAATTATTATCAAAGGTCGCTTGTATGTCCATTGTTTGTGCATATATATTGATTTTTCTTCTTCTTCATTTGATTGATGGATTAATTTTGGTTTGTGCATACATAAATAATGCAGTGTTCAAGTACCCCCCTTCAGGAAATTCCACGGTTGTTCCCAGCGTTTACTTGCTACCGAATATGTGGAGTTACACGACGTGTGAATTTAGAGGAGCAAAGTTGCTTGGTAATGCTGATCAAGGTGGTGGTGAGGGCATAAAGATTGAGCTGAATCAGTGGAAGCCTTATTACTTTGCCTCTGATGAAGGCAATGCCTATGACTGTATTGCTGGACTCACCAAGTTCATTGCTGTTCCATCTACACGTTCCTTTTAA

Protein sequence

>MS.gene38741.t1

MSRILIQGVIFLVIASMVAGSGSKARTKRSILVGDSEGWRAGTNYTQWAIKNSPFQINDTLVFKYPPSGNSTVVPSVYLLPNMWSYTTCEFRGAKLLGNADQGGGEGIKIELNQWKPYYFASDEGNAYDCIAGLTKFIAVPSTRSF