AlfalfaGEDB | MS.gene51147

M. sativa cultivar XinJiangDaYe / MS.gene51147

KEGG: K03671
GO: GO:0008150 GO:0009987 GO:0019725 GO:0042592 GO:0045454 GO:0050789 GO:0050794 GO:0065007 GO:0065008
PFAM: PF00085.23

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51147.t1	AES80410.1	97.4	77	2	0	9	85	106	182	6.00E-35	156.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51147.t1	P29450	89.6	77	8	0	9	85	106	182	7.4e-34	144.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51147.t1	B7FGW0	97.4	77	2	0	9	85	110	186	4.3e-35	156.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene51147	0.830646	2.61E-55	-1.69E-46
MS.gene049232	MS.gene51147	0.84104	6.05E-58	-1.69E-46
MS.gene049285	MS.gene51147	0.847457	1.14E-59	-1.69E-46
MS.gene049320	MS.gene51147	0.829486	5.01E-55	-1.69E-46
MS.gene049865	MS.gene51147	0.81052	1.11E-50	-1.69E-46
MS.gene05010	MS.gene51147	0.852882	3.45E-61	-1.69E-46
MS.gene050170	MS.gene51147	0.827851	1.24E-54	-1.69E-46
MS.gene050323	MS.gene51147	0.843827	1.10E-58	-1.69E-46
MS.gene050333	MS.gene51147	0.814851	1.25E-51	-1.69E-46
MS.gene050496	MS.gene51147	0.870635	1.26E-66	-1.69E-46
MS.gene050504	MS.gene51147	0.851544	8.28E-61	-1.69E-46
MS.gene050518	MS.gene51147	0.827554	1.47E-54	-1.69E-46
MS.gene050612	MS.gene51147	0.857394	1.68E-62	-1.69E-46
MS.gene050675	MS.gene51147	0.852167	5.51E-61	-1.69E-46
MS.gene050699	MS.gene51147	0.849608	2.90E-60	-1.69E-46
MS.gene050772	MS.gene51147	0.824854	6.44E-54	-1.69E-46
MS.gene050868	MS.gene51147	0.810026	1.41E-50	-1.69E-46
MS.gene050871	MS.gene51147	0.809477	1.86E-50	-1.69E-46
MS.gene050876	MS.gene51147	0.850059	2.17E-60	-1.69E-46
MS.gene050904	MS.gene51147	0.801842	7.44E-49	-1.69E-46
MS.gene050986	MS.gene51147	0.853431	2.40E-61	-1.69E-46
MS.gene051317	MS.gene51147	0.827948	1.18E-54	-1.69E-46
MS.gene051551	MS.gene51147	0.820804	5.65E-53	-1.69E-46
MS.gene051810	MS.gene51147	0.809848	1.54E-50	-1.69E-46
MS.gene052486	MS.gene51147	0.812588	3.93E-51	-1.69E-46
MS.gene052966	MS.gene51147	0.834929	2.26E-56	-1.69E-46
MS.gene053213	MS.gene51147	0.804343	2.26E-49	-1.69E-46
MS.gene053286	MS.gene51147	0.806755	7.05E-50	-1.69E-46
MS.gene053479	MS.gene51147	0.8067	7.24E-50	-1.69E-46
MS.gene053480	MS.gene51147	0.80975	1.62E-50	-1.69E-46
MS.gene053633	MS.gene51147	0.823211	1.56E-53	-1.69E-46
MS.gene053824	MS.gene51147	0.821519	3.87E-53	-1.69E-46
MS.gene054600	MS.gene51147	0.818294	2.11E-52	-1.69E-46
MS.gene054642	MS.gene51147	0.811109	8.25E-51	-1.69E-46
MS.gene054954	MS.gene51147	0.866998	1.89E-65	-1.69E-46
MS.gene055362	MS.gene51147	0.852171	5.50E-61	-1.69E-46
MS.gene055502	MS.gene51147	0.839404	1.62E-57	-1.69E-46
MS.gene055577	MS.gene51147	0.817625	2.99E-52	-1.69E-46
MS.gene055634	MS.gene51147	0.816889	4.38E-52	-1.69E-46
MS.gene055636	MS.gene51147	0.820548	6.47E-53	-1.69E-46
MS.gene055638	MS.gene51147	0.821566	3.77E-53	-1.69E-46
MS.gene055722	MS.gene51147	0.803812	2.92E-49	-1.69E-46
MS.gene055750	MS.gene51147	0.803564	3.28E-49	-1.69E-46
MS.gene055776	MS.gene51147	0.835592	1.53E-56	-1.69E-46
MS.gene05589	MS.gene51147	0.814684	1.36E-51	-1.69E-46
MS.gene055941	MS.gene51147	0.812747	3.63E-51	-1.69E-46
MS.gene056046	MS.gene51147	0.800331	1.52E-48	-1.69E-46
MS.gene056504	MS.gene51147	0.865855	4.36E-65	-1.69E-46
MS.gene056510	MS.gene51147	0.864945	8.44E-65	-1.69E-46
MS.gene056657	MS.gene51147	0.839343	1.68E-57	-1.69E-46
MS.gene057857	MS.gene51147	0.803013	4.27E-49	-1.69E-46
MS.gene05837	MS.gene51147	0.808784	2.61E-50	-1.69E-46
MS.gene058419	MS.gene51147	0.83976	1.31E-57	-1.69E-46
MS.gene058773	MS.gene51147	0.831645	1.48E-55	-1.69E-46
MS.gene059022	MS.gene51147	0.86462	1.07E-64	-1.69E-46
MS.gene059023	MS.gene51147	0.826478	2.65E-54	-1.69E-46
MS.gene05912	MS.gene51147	0.800638	1.31E-48	-1.69E-46
MS.gene059677	MS.gene51147	0.832055	1.18E-55	-1.69E-46
MS.gene059718	MS.gene51147	0.811031	8.58E-51	-1.69E-46
MS.gene059756	MS.gene51147	0.802005	6.89E-49	-1.69E-46
MS.gene059837	MS.gene51147	0.819262	1.27E-52	-1.69E-46
MS.gene059865	MS.gene51147	0.849234	3.69E-60	-1.69E-46
MS.gene06000	MS.gene51147	0.822341	2.49E-53	-1.69E-46
MS.gene060595	MS.gene51147	-0.801434	9.02E-49	-1.69E-46
MS.gene060703	MS.gene51147	0.884169	2.41E-71	-1.69E-46
MS.gene060762	MS.gene51147	0.814175	1.76E-51	-1.69E-46
MS.gene060778	MS.gene51147	0.849715	2.71E-60	-1.69E-46
MS.gene06077	MS.gene51147	0.842608	2.33E-58	-1.69E-46
MS.gene060982	MS.gene51147	0.851841	6.82E-61	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51147.t1	MTR_7g080250	90.476	84	6	1	2	85	101	182	1.24e-50	157
MS.gene51147.t1	MTR_5g021180	41.667	72	41	1	9	80	42	112	1.25e-14	63.9
MS.gene51147.t1	MTR_1g023140	45.070	71	38	1	9	79	40	109	7.79e-14	62.0
MS.gene51147.t1	MTR_3g112410	43.662	71	39	1	9	79	40	109	5.33e-13	59.7
MS.gene51147.t1	MTR_7g009070	40.299	67	39	1	9	75	60	125	3.93e-12	57.8
MS.gene51147.t1	MTR_7g073680	41.791	67	38	1	9	75	58	123	7.81e-11	54.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51147.t1	AT5G16400	79.762	84	15	1	2	85	104	185	3.46e-43	138
MS.gene51147.t1	AT3G02730	80.952	84	14	1	2	85	94	175	3.62e-43	138
MS.gene51147.t1	AT3G51030	44.776	67	36	1	9	75	40	105	2.14e-14	63.2
MS.gene51147.t1	AT5G42980	38.235	68	41	1	9	76	39	105	8.51e-13	59.3
MS.gene51147.t1	AT5G39950	38.961	77	44	2	9	84	59	133	4.08e-12	57.8
MS.gene51147.t1	AT1G45145	35.821	67	42	1	9	75	39	104	4.74e-12	57.4
MS.gene51147.t1	AT1G19730	38.235	68	40	2	9	75	40	106	2.63e-11	55.5

Find 15 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCAGCTAATTCTTTGTATTT+TGG	0.153277	7.3:+27253833	None:intergenic
AGAATTAGCTGAGAAGTATT+TGG	0.241894	7.3:-27253822	MS.gene51147:CDS
CACCTTAATTCCTAGCTCTT+TGG	0.269465	7.3:+27253662	None:intergenic
TAATCCATACCTTGTTATCT+TGG	0.303298	7.3:+27253771	None:intergenic
TGGAGCTAAATATGATGATT+TGG	0.335332	7.3:-27253589	MS.gene51147:CDS
GGCCAAAGAGCTAGGAATTA+AGG	0.347175	7.3:-27253664	MS.gene51147:CDS
AAGGTTGTAAAAGAAGTAAC+TGG	0.408021	7.3:-27253609	MS.gene51147:CDS
AATTCCTAGCTCTTTGGCCA+AGG	0.431968	7.3:+27253668	None:intergenic
ATTCCTAGCTCTTTGGCCAA+GGG	0.487064	7.3:+27253669	None:intergenic
CAGCCCTTGGCCAAAGAGCT+AGG	0.508539	7.3:-27253672	MS.gene51147:CDS
GCTAGAACGAACTGTGTCAA+TGG	0.511358	7.3:+27253560	None:intergenic
TTTGGAGCTATCACTTTGCA+AGG	0.518801	7.3:+27253851	None:intergenic
TAAAATTCTGAAAGACAACA+AGG	0.632667	7.3:-27253628	MS.gene51147:CDS
CAAAGAGCTAGGAATTAAGG+TGG	0.670646	7.3:-27253661	MS.gene51147:CDS
TGATTGCAACCAAGATAACA+AGG	0.674067	7.3:-27253780	MS.gene51147:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TCTTTCAGAATTTTAAAAGT+GGG	+	chr7.3:27253805-27253824	None:intergenic	20.0%
!	ACATAGTGTTTAATGGTTAT+TGG	-	chr7.3:27253743-27253762	MS.gene51147:intron	25.0%
!!	TAAAATTCTGAAAGACAACA+AGG	-	chr7.3:27253810-27253829	MS.gene51147:CDS	25.0%
!!	TCAGCTAATTCTTTGTATTT+TGG	+	chr7.3:27253608-27253627	None:intergenic	25.0%
!!	TGATTTACAATGAATTTTGC+AGG	-	chr7.3:27253558-27253577	MS.gene51147:CDS	25.0%
!!!	GTCTTTCAGAATTTTAAAAG+TGG	+	chr7.3:27253806-27253825	None:intergenic	25.0%
	AAGGTTGTAAAAGAAGTAAC+TGG	-	chr7.3:27253829-27253848	MS.gene51147:CDS	30.0%
	ACTGAATAGTAGATAGTCTA+TGG	-	chr7.3:27253698-27253717	MS.gene51147:intron	30.0%
	TAACCATTAAACACTATGTC+AGG	+	chr7.3:27253742-27253761	None:intergenic	30.0%
	TGGAGCTAAATATGATGATT+TGG	-	chr7.3:27253849-27253868	MS.gene51147:CDS	30.0%
!	TAATCCATACCTTGTTATCT+TGG	+	chr7.3:27253670-27253689	None:intergenic	30.0%
!!	AGAATTAGCTGAGAAGTATT+TGG	-	chr7.3:27253616-27253635	MS.gene51147:CDS	30.0%
	TGATTGCAACCAAGATAACA+AGG	-	chr7.3:27253658-27253677	MS.gene51147:CDS	35.0%
!	TACAATGAATTTTGCAGGTG+TGG	-	chr7.3:27253563-27253582	MS.gene51147:CDS	35.0%
	CAAAGAGCTAGGAATTAAGG+TGG	-	chr7.3:27253777-27253796	MS.gene51147:intron	40.0%
	GCAACCAAGATAACAAGGTA+TGG	-	chr7.3:27253663-27253682	MS.gene51147:CDS	40.0%
	TGGCCTGACATAGTGTTTAA+TGG	-	chr7.3:27253736-27253755	MS.gene51147:intron	40.0%
!	CACCTTAATTCCTAGCTCTT+TGG	+	chr7.3:27253779-27253798	None:intergenic	40.0%
!	TTTGGAGCTATCACTTTGCA+AGG	+	chr7.3:27253590-27253609	None:intergenic	40.0%
	GCTAGAACGAACTGTGTCAA+TGG	+	chr7.3:27253881-27253900	None:intergenic	45.0%
	GGCCAAAGAGCTAGGAATTA+AGG	-	chr7.3:27253774-27253793	MS.gene51147:intron	45.0%
	TAATGGTTATTGGCAGCCCT+TGG	-	chr7.3:27253753-27253772	MS.gene51147:intron	45.0%
	TATGGTTGCCCATGCTTTCT+TGG	-	chr7.3:27253716-27253735	MS.gene51147:intron	45.0%
	TATGTCAGGCCAAGAAAGCA+TGG	+	chr7.3:27253728-27253747	None:intergenic	45.0%
!	AATTCCTAGCTCTTTGGCCA+AGG	+	chr7.3:27253773-27253792	None:intergenic	45.0%
!	ATGTCAGGCCAAGAAAGCAT+GGG	+	chr7.3:27253727-27253746	None:intergenic	45.0%
!	ATTCCTAGCTCTTTGGCCAA+GGG	+	chr7.3:27253772-27253791	None:intergenic	45.0%
	CAGCCCTTGGCCAAAGAGCT+AGG	-	chr7.3:27253766-27253785	MS.gene51147:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	27253557	27253903	27253557	ID=MS.gene51147
chr7.3	mRNA	27253557	27253903	27253557	ID=MS.gene51147.t1;Parent=MS.gene51147
chr7.3	exon	27253781	27253903	27253781	ID=MS.gene51147.t1.exon1;Parent=MS.gene51147.t1
chr7.3	CDS	27253781	27253903	27253781	ID=cds.MS.gene51147.t1;Parent=MS.gene51147.t1
chr7.3	exon	27253557	27253691	27253557	ID=MS.gene51147.t1.exon2;Parent=MS.gene51147.t1
chr7.3	CDS	27253557	27253691	27253557	ID=cds.MS.gene51147.t1;Parent=MS.gene51147.t1

Gene Sequence	>MS.gene51147 ATGATTTACAATGAATTTTGCAGGTGTGGTCCTTGCAAAGTGATAGCTCCAAAATACAAAGAATTAGCTGAGAAGTATTTGGATGTTGTCTTTCTAAAGCTTGATTGCAACCAAGATAACAAGGTATGGATTAAATAATGTACTGAATAGTAGATAGTCTATGGTTGCCCATGCTTTCTTGGCCTGACATAGTGTTTAATGGTTATTGGCAGCCCTTGGCCAAAGAGCTAGGAATTAAGGTGGTTCCCACTTTTAAAATTCTGAAAGACAACAAGGTTGTAAAAGAAGTAACTGGAGCTAAATATGATGATTTGGTCTTTGCCATTGACACAGTTCGTTCTAGCTAA
Protein sequence	>MS.gene51147.t1 MIYNEFCRCGPCKVIAPKYKELAEKYLDVVFLKLDCNQDNKPLAKELGIKVVPTFKILKDNKVVKEVTGAKYDDLVFAIDTVRSS