AlfalfaGEDB | MS.gene58652

M. sativa cultivar XinJiangDaYe / MS.gene58652

KEGG: K13260
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005506 GO:0008150 GO:0008152 GO:0016491 GO:0016705 GO:0020037 GO:0043167 GO:0043169 GO:0046872 GO:0046906 GO:0046914 GO:0048037 GO:0055114 GO:0097159 GO:1901363
PFAM: PF00067.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58652.t1	XP_013463333.1	94.4	144	8	0	1	144	159	302	8.80E-71	276.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58652.t1	Q6WNQ9	93.8	144	9	0	1	144	157	300	1.8e-72	273.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58652.t1	A0A072V5R8	94.4	144	8	0	1	144	159	302	6.3e-71	276.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049150	MS.gene58652	0.838565	2.67E-57	-1.69E-46
MS.gene049151	MS.gene58652	0.818079	2.36E-52	-1.69E-46
MS.gene049153	MS.gene58652	0.834302	3.24E-56	-1.69E-46
MS.gene049378	MS.gene58652	0.800147	1.65E-48	-1.69E-46
MS.gene049577	MS.gene58652	0.807135	5.86E-50	-1.69E-46
MS.gene050060	MS.gene58652	0.853764	1.93E-61	-1.69E-46
MS.gene050133	MS.gene58652	-0.813379	2.64E-51	-1.69E-46
MS.gene050426	MS.gene58652	-0.833508	5.12E-56	-1.69E-46
MS.gene050642	MS.gene58652	0.841136	5.71E-58	-1.69E-46
MS.gene050680	MS.gene58652	0.802969	4.36E-49	-1.69E-46
MS.gene051373	MS.gene58652	0.841012	6.15E-58	-1.69E-46
MS.gene051535	MS.gene58652	0.802034	6.79E-49	-1.69E-46
MS.gene051983	MS.gene58652	0.863751	1.99E-64	-1.69E-46
MS.gene052011	MS.gene58652	-0.807443	5.04E-50	-1.69E-46
MS.gene052077	MS.gene58652	0.835218	1.91E-56	-1.69E-46
MS.gene052176	MS.gene58652	0.810396	1.18E-50	-1.69E-46
MS.gene052188	MS.gene58652	0.828701	7.76E-55	-1.69E-46
MS.gene052340	MS.gene58652	0.845624	3.62E-59	-1.69E-46
MS.gene052389	MS.gene58652	0.803197	3.91E-49	-1.69E-46
MS.gene052469	MS.gene58652	0.81774	2.82E-52	-1.69E-46
MS.gene052590	MS.gene58652	0.809977	1.45E-50	-1.69E-46
MS.gene052758	MS.gene58652	0.811553	6.61E-51	-1.69E-46
MS.gene053073	MS.gene58652	0.810457	1.14E-50	-1.69E-46
MS.gene05316	MS.gene58652	0.809994	1.44E-50	-1.69E-46
MS.gene053330	MS.gene58652	0.832534	8.95E-56	-1.69E-46
MS.gene053494	MS.gene58652	0.84469	6.47E-59	-1.69E-46
MS.gene053928	MS.gene58652	0.837414	5.27E-57	-1.69E-46
MS.gene054102	MS.gene58652	0.805331	1.41E-49	-1.69E-46
MS.gene054183	MS.gene58652	0.825287	5.09E-54	-1.69E-46
MS.gene054349	MS.gene58652	0.852442	4.61E-61	-1.69E-46
MS.gene054556	MS.gene58652	0.805914	1.06E-49	-1.69E-46
MS.gene054930	MS.gene58652	0.840017	1.12E-57	-1.69E-46
MS.gene05510	MS.gene58652	0.801118	1.05E-48	-1.69E-46
MS.gene055367	MS.gene58652	0.82138	4.16E-53	-1.69E-46
MS.gene055539	MS.gene58652	0.809848	1.54E-50	-1.69E-46
MS.gene05553	MS.gene58652	0.880486	5.28E-70	-1.69E-46
MS.gene05556	MS.gene58652	0.810916	9.09E-51	-1.69E-46
MS.gene055701	MS.gene58652	0.855292	6.96E-62	-1.69E-46
MS.gene056233	MS.gene58652	0.816091	6.61E-52	-1.69E-46
MS.gene056659	MS.gene58652	0.823098	1.66E-53	-1.69E-46
MS.gene056723	MS.gene58652	0.829106	6.20E-55	-1.69E-46
MS.gene056899	MS.gene58652	-0.801413	9.11E-49	-1.69E-46
MS.gene056979	MS.gene58652	0.858878	6.09E-63	-1.69E-46
MS.gene057156	MS.gene58652	-0.806553	7.78E-50	-1.69E-46
MS.gene057396	MS.gene58652	0.867993	9.08E-66	-1.69E-46
MS.gene057825	MS.gene58652	0.800341	1.51E-48	-1.69E-46
MS.gene058002	MS.gene58652	0.805403	1.36E-49	-1.69E-46
MS.gene058142	MS.gene58652	0.873259	1.69E-67	-1.69E-46
MS.gene058276	MS.gene58652	0.831943	1.25E-55	-1.69E-46
MS.gene058371	MS.gene58652	0.801887	7.28E-49	-1.69E-46
MS.gene058435	MS.gene58652	0.816979	4.18E-52	-1.69E-46
MS.gene058949	MS.gene58652	0.820242	7.61E-53	-1.69E-46
MS.gene059011	MS.gene58652	0.840656	7.62E-58	-1.69E-46
MS.gene059247	MS.gene58652	0.866449	2.83E-65	-1.69E-46
MS.gene059285	MS.gene58652	0.844819	5.97E-59	-1.69E-46
MS.gene059420	MS.gene58652	0.807202	5.67E-50	-1.69E-46
MS.gene059609	MS.gene58652	0.807303	5.40E-50	-1.69E-46
MS.gene059710	MS.gene58652	0.811431	7.03E-51	-1.69E-46
MS.gene059788	MS.gene58652	0.849772	2.61E-60	-1.69E-46
MS.gene059894	MS.gene58652	0.803453	3.46E-49	-1.69E-46
MS.gene059899	MS.gene58652	0.80675	7.07E-50	-1.69E-46
MS.gene060083	MS.gene58652	0.815862	7.44E-52	-1.69E-46
MS.gene060247	MS.gene58652	0.809052	2.29E-50	-1.69E-46
MS.gene060407	MS.gene58652	0.834957	2.22E-56	-1.69E-46
MS.gene061049	MS.gene58652	0.808649	2.79E-50	-1.69E-46
MS.gene061114	MS.gene58652	-0.82769	1.36E-54	-1.69E-46
MS.gene061159	MS.gene58652	0.856975	2.24E-62	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58652.t1	MTR_2g437880	94.444	144	8	0	1	144	159	302	1.53e-94	282
MS.gene58652.t1	MTR_5g016410	66.165	133	43	1	11	143	166	296	4.31e-56	182
MS.gene58652.t1	MTR_4g094775	59.542	131	51	1	12	142	167	295	1.00e-50	168
MS.gene58652.t1	MTR_4g095050	59.701	134	51	2	11	143	169	300	1.49e-49	166
MS.gene58652.t1	MTR_2g437840	55.303	132	57	1	12	143	167	296	4.05e-43	148
MS.gene58652.t1	MTR_4g035330	53.731	134	49	2	11	144	165	285	9.26e-41	142
MS.gene58652.t1	MTR_4g094772	52.632	133	50	2	12	144	169	288	5.45e-40	140
MS.gene58652.t1	MTR_5g016440	46.970	132	64	2	12	143	166	291	1.70e-33	122
MS.gene58652.t1	MTR_4g077210	36.842	133	80	2	12	143	172	301	2.72e-25	100
MS.gene58652.t1	MTR_7g087520	34.211	152	90	3	1	144	160	309	5.69e-18	79.7
MS.gene58652.t1	MTR_6g008650	34.783	115	73	1	7	121	177	289	1.73e-16	75.5
MS.gene58652.t1	MTR_6g008500	38.318	107	65	1	2	108	183	288	6.23e-16	73.6
MS.gene58652.t1	MTR_4g109470	32.653	147	92	4	1	143	154	297	2.55e-15	72.0
MS.gene58652.t1	MTR_3g025260	35.821	134	79	4	15	143	169	300	3.22e-15	71.6
MS.gene58652.t1	MTR_8g020940	33.913	115	71	3	17	128	173	285	8.21e-15	70.5
MS.gene58652.t1	MTR_6g008640	33.846	130	80	3	7	131	177	305	8.27e-15	70.5
MS.gene58652.t1	MTR_8g020960	34.188	117	72	3	17	130	174	288	1.11e-14	70.1
MS.gene58652.t1	MTR_8g020950	33.621	116	72	3	17	129	175	288	1.33e-14	69.7
MS.gene58652.t1	MTR_3g024520	35.644	101	64	1	15	115	169	268	1.85e-14	69.3
MS.gene58652.t1	MTR_3g025230	35.644	101	64	1	15	115	169	268	1.94e-14	69.3
MS.gene58652.t1	MTR_6g042610	35.514	107	66	2	11	117	175	278	3.26e-14	68.9
MS.gene58652.t1	MTR_6g042540	30.435	138	88	3	11	141	175	311	7.39e-14	67.8
MS.gene58652.t1	MTR_2g450040	35.965	114	66	2	2	111	165	275	1.12e-13	67.4
MS.gene58652.t1	MTR_5g018480	31.579	133	80	3	15	141	185	312	1.31e-13	66.6
MS.gene58652.t1	MTR_6g008620	31.618	136	83	3	15	143	189	321	2.76e-13	66.2
MS.gene58652.t1	MTR_4g088425	31.034	116	74	1	15	130	176	285	4.61e-13	65.5
MS.gene58652.t1	MTR_3g014880	33.028	109	72	1	7	115	178	285	5.92e-13	65.1
MS.gene58652.t1	MTR_0638s0020	31.618	136	81	5	1	133	90	216	1.15e-12	64.3
MS.gene58652.t1	MTR_6g008600	31.429	140	87	4	12	143	185	323	2.40e-12	63.5
MS.gene58652.t1	MTR_5g045250	33.600	125	74	2	15	136	194	312	5.17e-12	62.4
MS.gene58652.t1	MTR_8g091690	32.520	123	75	3	13	133	161	277	7.26e-12	62.0
MS.gene58652.t1	MTR_3g025250	37.037	81	50	1	35	115	3	82	8.52e-12	61.2
MS.gene58652.t1	MTR_6g008530	31.915	141	86	5	12	143	186	325	2.39e-11	60.5
MS.gene58652.t1	MTR_3g436390	29.060	117	71	3	1	111	159	269	5.68e-11	59.3
MS.gene58652.t1	MTR_4g018800	29.358	109	76	1	7	115	179	286	6.13e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene58652.t1	AT4G37340	50.000	132	61	3	12	143	168	294	3.51e-36	130
MS.gene58652.t1	AT4G37370	48.462	130	61	3	12	141	168	291	1.28e-35	128
MS.gene58652.t1	AT1G66540	48.462	130	62	3	12	141	55	179	6.12e-34	122
MS.gene58652.t1	AT2G23220	47.368	133	64	3	11	143	183	309	7.30e-34	124
MS.gene58652.t1	AT3G28740	46.617	133	65	4	11	143	176	302	1.17e-32	120
MS.gene58652.t1	AT4G37310	47.727	132	65	3	14	143	170	299	1.97e-32	120
MS.gene58652.t1	AT4G37430	43.796	137	71	3	7	143	164	294	8.86e-32	118
MS.gene58652.t1	AT1G66540	47.200	125	61	3	17	141	1	120	2.67e-31	114
MS.gene58652.t1	AT5G36220	45.802	131	65	4	13	143	30	154	2.88e-31	114
MS.gene58652.t1	AT4G37360	45.926	135	68	3	7	141	163	292	9.97e-31	115
MS.gene58652.t1	AT5G36220	45.802	131	65	4	13	143	176	300	2.38e-30	114
MS.gene58652.t1	AT4G37320	43.609	133	69	3	11	143	168	294	8.02e-29	110
MS.gene58652.t1	AT4G37400	42.308	130	69	3	14	143	166	289	8.15e-29	110
MS.gene58652.t1	AT2G23190	40.602	133	73	3	11	143	212	338	2.80e-28	108
MS.gene58652.t1	AT4G37330	42.105	133	68	3	11	143	168	291	4.61e-28	107
MS.gene58652.t1	AT5G67310	40.845	142	74	3	4	144	174	306	5.17e-28	107
MS.gene58652.t1	AT5G57220	43.411	129	67	3	15	143	167	289	2.47e-27	105
MS.gene58652.t1	AT4G37410	43.077	130	68	3	14	143	168	291	4.73e-26	102
MS.gene58652.t1	AT5G10600	36.641	131	77	3	14	143	184	309	7.51e-19	82.0
MS.gene58652.t1	AT4G31940	37.681	138	75	4	2	128	159	296	1.61e-18	81.3
MS.gene58652.t1	AT1G01280	33.557	149	93	4	1	144	155	302	2.51e-17	77.8
MS.gene58652.t1	AT5G10610	34.884	129	79	2	15	143	170	293	1.37e-15	72.8
MS.gene58652.t1	AT4G31970	34.454	119	73	2	15	128	177	295	3.35e-15	71.6
MS.gene58652.t1	AT4G31950	33.824	136	80	3	15	140	165	300	5.05e-15	71.2
MS.gene58652.t1	AT5G07990	32.759	116	74	1	11	126	164	275	1.00e-14	70.5

Find 19 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGAAGAAGCTAAACAATTT+AGG	0.153734	2.3:-53836751	MS.gene58652:CDS
TGAAGAAGCTAAACAATTTA+GGG	0.292562	2.3:-53836750	MS.gene58652:CDS
TTTGTTAGGTGCTAATAATA+AGG	0.319600	2.3:-53836705	MS.gene58652:CDS
TGAGAATGATATCGGGAAAA+AGG	0.333160	2.3:-53836805	MS.gene58652:CDS
TGCTATGATGAGAATGATAT+CGG	0.334134	2.3:-53836813	MS.gene58652:CDS
AGTGAGATTATGTCTTTGTT+AGG	0.341716	2.3:-53836719	MS.gene58652:CDS
TTTAATCAAAGGTCTAATTC+AGG	0.349158	2.3:-53836483	None:intergenic
AAAAGGTATTATGGAGATGA+TGG	0.449764	2.3:-53836788	MS.gene58652:CDS
ATCTTGATAATTTGGAAAAG+AGG	0.455960	2.3:-53836652	MS.gene58652:CDS
TTGTTAGGTGCTAATAATAA+GGG	0.469324	2.3:-53836704	MS.gene58652:CDS
AGGTTTCGGTTTCACTAAAG+TGG	0.489603	2.3:-53836870	MS.gene58652:CDS
GACGTCGTCTCTGAAGGTTT+CGG	0.493391	2.3:-53836884	MS.gene58652:CDS
CTCAAAGACGTCGTCTCTGA+AGG	0.511853	2.3:-53836890	MS.gene58652:CDS
TATTCAGATCATTTAATCAA+AGG	0.521355	2.3:-53836494	MS.gene58652:CDS
GCTATGATGAGAATGATATC+GGG	0.563969	2.3:-53836812	MS.gene58652:CDS
AAATGATCTGAATAATACTC+AGG	0.575038	2.3:+53836503	None:intergenic
AGAGGAAATATTCGTAGTGA+TGG	0.578649	2.3:-53836569	MS.gene58652:CDS
GGAAATATTCGTAGTGATGG+AGG	0.597253	2.3:-53836566	MS.gene58652:CDS
CTCATTGAGGAACATCGCAG+AGG	0.707653	2.3:-53836587	MS.gene58652:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATTCAGATCATTTAATCAA+AGG	-	chr2.3:53836883-53836902	MS.gene58652:CDS	20.0%
!!!	GTTTTTTGATCTTGATAATT+TGG	-	chr2.3:53836717-53836736	MS.gene58652:CDS	20.0%
!	AAATGATCTGAATAATACTC+AGG	+	chr2.3:53836877-53836896	None:intergenic	25.0%
!	TGAAGAAGCTAAACAATTTA+GGG	-	chr2.3:53836627-53836646	MS.gene58652:CDS	25.0%
!	TTGAAGAAGCTAAACAATTT+AGG	-	chr2.3:53836626-53836645	MS.gene58652:CDS	25.0%
!	TTGTTAGGTGCTAATAATAA+GGG	-	chr2.3:53836673-53836692	MS.gene58652:CDS	25.0%
!	TTTGTTAGGTGCTAATAATA+AGG	-	chr2.3:53836672-53836691	MS.gene58652:CDS	25.0%
!!	AAAAAGATCTGATGCATTTT+TGG	-	chr2.3:53836762-53836781	MS.gene58652:CDS	25.0%
!!	ATCTTGATAATTTGGAAAAG+AGG	-	chr2.3:53836725-53836744	MS.gene58652:CDS	25.0%
	AGATCAAAAAACCTTAACAG+AGG	+	chr2.3:53836709-53836728	None:intergenic	30.0%
	AGTGAGATTATGTCTTTGTT+AGG	-	chr2.3:53836658-53836677	MS.gene58652:CDS	30.0%
	TGCTATGATGAGAATGATAT+CGG	-	chr2.3:53836564-53836583	MS.gene58652:CDS	30.0%
!!	AAAAGGTATTATGGAGATGA+TGG	-	chr2.3:53836589-53836608	MS.gene58652:CDS	30.0%
!!	ATATCGGGAAAAAGGTATTA+TGG	-	chr2.3:53836580-53836599	MS.gene58652:CDS	30.0%
!!!	ATTGATCATCTTTTGAAGCT+TGG	-	chr2.3:53836841-53836860	MS.gene58652:CDS	30.0%
	AGAGGAAATATTCGTAGTGA+TGG	-	chr2.3:53836808-53836827	MS.gene58652:CDS	35.0%
	GCTATGATGAGAATGATATC+GGG	-	chr2.3:53836565-53836584	MS.gene58652:CDS	35.0%
	TGAGAATGATATCGGGAAAA+AGG	-	chr2.3:53836572-53836591	MS.gene58652:CDS	35.0%
!!	AAGATCTGATGCATTTTTGG+AGG	-	chr2.3:53836765-53836784	MS.gene58652:CDS	35.0%
!!	AGATCTGATGCATTTTTGGA+GGG	-	chr2.3:53836766-53836785	MS.gene58652:CDS	35.0%
!!!	GTGATTTTTTGCCTCTGTTA+AGG	-	chr2.3:53836695-53836714	MS.gene58652:CDS	35.0%
	AGGTTTCGGTTTCACTAAAG+TGG	-	chr2.3:53836507-53836526	MS.gene58652:CDS	40.0%
	GGAAATATTCGTAGTGATGG+AGG	-	chr2.3:53836811-53836830	MS.gene58652:CDS	40.0%
!!	ATTTTTGGAGGGACTCATTG+AGG	-	chr2.3:53836777-53836796	MS.gene58652:CDS	40.0%
	CTCAAAGACGTCGTCTCTGA+AGG	-	chr2.3:53836487-53836506	MS.gene58652:CDS	50.0%
	CTCATTGAGGAACATCGCAG+AGG	-	chr2.3:53836790-53836809	MS.gene58652:CDS	50.0%
!	GACGTCGTCTCTGAAGGTTT+CGG	-	chr2.3:53836493-53836512	MS.gene58652:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	53836484	53836915	53836484	ID=MS.gene58652
chr2.3	mRNA	53836484	53836915	53836484	ID=MS.gene58652.t1;Parent=MS.gene58652
chr2.3	exon	53836484	53836915	53836484	ID=MS.gene58652.t1.exon1;Parent=MS.gene58652.t1
chr2.3	CDS	53836484	53836915	53836484	ID=cds.MS.gene58652.t1;Parent=MS.gene58652.t1

Gene Sequence

>MS.gene58652

CTTCTCAAAGACGTCGTCTCTGAAGGTTTCGGTTTCACTAAAGTGGAACTGAGATCGAGACTAACAGAGATGACGTTTAATGCTATGATGAGAATGATATCGGGAAAAAGGTATTATGGAGATGATGGAGACGTGACAGATGTTGAAGAAGCTAAACAATTTAGGGAGATAATAAGTGAGATTATGTCTTTGTTAGGTGCTAATAATAAGGGTGATTTTTTGCCTCTGTTAAGGTTTTTTGATCTTGATAATTTGGAAAAGAGGTGCAAGAAGATTGCAAAAAGATCTGATGCATTTTTGGAGGGACTCATTGAGGAACATCGCAGAGGAAATATTCGTAGTGATGGAGGTACAATGATTGATCATCTTTTGAAGCTTGGTGAATCACAACCTGAGTATTATTCAGATCATTTAATCAAAGGTCTAATTCAG

Protein sequence

>MS.gene58652.t1

LLKDVVSEGFGFTKVELRSRLTEMTFNAMMRMISGKRYYGDDGDVTDVEEAKQFREIISEIMSLLGANNKGDFLPLLRFFDLDNLEKRCKKIAKRSDAFLEGLIEEHRRGNIRSDGGTMIDHLLKLGESQPEYYSDHLIKGLIQ