AlfalfaGEDB | MS.gene59937

M. sativa cultivar XinJiangDaYe / MS.gene59937

KEGG: K14484
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF02309.19

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59937.t1	XP_013458098.1	92.3	183	13	1	1	183	1	182	5.20E-76	293.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59937.t1	Q9XFM0	37.6	93	52	2	93	183	49	137	5.2e-08	59.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59937.t1	A0A072UQL9	92.3	183	13	1	1	183	1	182	3.7e-76	293.9

TFs/TRs:

Gene ID	Type	Classification
MS.gene59937.t1	TR	AUX/IAA

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049171	MS.gene59937	0.822635	2.13E-53	-1.69E-46
MS.gene049173	MS.gene59937	0.804743	1.87E-49	-1.69E-46
MS.gene049217	MS.gene59937	0.836717	7.95E-57	-1.69E-46
MS.gene049222	MS.gene59937	0.823199	1.57E-53	-1.69E-46
MS.gene049285	MS.gene59937	0.84476	6.19E-59	-1.69E-46
MS.gene049320	MS.gene59937	0.848524	5.80E-60	-1.69E-46
MS.gene049633	MS.gene59937	0.804359	2.24E-49	-1.69E-46
MS.gene05010	MS.gene59937	0.804683	1.92E-49	-1.69E-46
MS.gene050163	MS.gene59937	0.827083	1.90E-54	-1.69E-46
MS.gene050165	MS.gene59937	0.824848	6.46E-54	-1.69E-46
MS.gene050333	MS.gene59937	0.815082	1.11E-51	-1.69E-46
MS.gene050444	MS.gene59937	0.808453	3.07E-50	-1.69E-46
MS.gene050504	MS.gene59937	0.832302	1.02E-55	-1.69E-46
MS.gene050518	MS.gene59937	0.817216	3.70E-52	-1.69E-46
MS.gene050577	MS.gene59937	0.802725	4.89E-49	-1.69E-46
MS.gene050612	MS.gene59937	0.843158	1.66E-58	-1.69E-46
MS.gene050699	MS.gene59937	0.826266	2.98E-54	-1.69E-46
MS.gene050772	MS.gene59937	0.825852	3.74E-54	-1.69E-46
MS.gene050986	MS.gene59937	0.831383	1.72E-55	-1.69E-46
MS.gene05100	MS.gene59937	0.800299	1.54E-48	-1.69E-46
MS.gene051228	MS.gene59937	0.830156	3.44E-55	-1.69E-46
MS.gene051551	MS.gene59937	0.820335	7.24E-53	-1.69E-46
MS.gene051810	MS.gene59937	0.841734	3.97E-58	-1.69E-46
MS.gene052271	MS.gene59937	0.829831	4.13E-55	-1.69E-46
MS.gene052379	MS.gene59937	0.813537	2.43E-51	-1.69E-46
MS.gene053104	MS.gene59937	0.811238	7.74E-51	-1.69E-46
MS.gene053207	MS.gene59937	0.809988	1.44E-50	-1.69E-46
MS.gene053213	MS.gene59937	0.823656	1.23E-53	-1.69E-46
MS.gene053286	MS.gene59937	0.825252	5.19E-54	-1.69E-46
MS.gene053322	MS.gene59937	0.801542	8.57E-49	-1.69E-46
MS.gene053632	MS.gene59937	0.838582	2.64E-57	-1.69E-46
MS.gene053916	MS.gene59937	0.801773	7.69E-49	-1.69E-46
MS.gene054595	MS.gene59937	0.806697	7.25E-50	-1.69E-46
MS.gene054600	MS.gene59937	0.828329	9.55E-55	-1.69E-46
MS.gene054642	MS.gene59937	0.858797	6.43E-63	-1.69E-46
MS.gene054954	MS.gene59937	0.829012	6.53E-55	-1.69E-46
MS.gene055206	MS.gene59937	0.829728	4.38E-55	-1.69E-46
MS.gene055362	MS.gene59937	0.840502	8.36E-58	-1.69E-46
MS.gene055502	MS.gene59937	0.821433	4.05E-53	-1.69E-46
MS.gene055577	MS.gene59937	0.813292	2.76E-51	-1.69E-46
MS.gene055636	MS.gene59937	0.805879	1.08E-49	-1.69E-46
MS.gene055638	MS.gene59937	0.803479	3.42E-49	-1.69E-46
MS.gene05592	MS.gene59937	0.80544	1.33E-49	-1.69E-46
MS.gene055941	MS.gene59937	0.809786	1.59E-50	-1.69E-46
MS.gene055956	MS.gene59937	-0.808733	2.68E-50	-1.69E-46
MS.gene056374	MS.gene59937	0.846508	2.08E-59	-1.69E-46
MS.gene056487	MS.gene59937	0.803636	3.17E-49	-1.69E-46
MS.gene056504	MS.gene59937	0.814551	1.45E-51	-1.69E-46
MS.gene056510	MS.gene59937	0.807932	3.97E-50	-1.69E-46
MS.gene056657	MS.gene59937	0.84712	1.41E-59	-1.69E-46
MS.gene057129	MS.gene59937	0.826077	3.31E-54	-1.69E-46
MS.gene057416	MS.gene59937	0.815403	9.41E-52	-1.69E-46
MS.gene058419	MS.gene59937	0.84474	6.27E-59	-1.69E-46
MS.gene058544	MS.gene59937	0.810342	1.21E-50	-1.69E-46
MS.gene058723	MS.gene59937	0.807887	4.06E-50	-1.69E-46
MS.gene058773	MS.gene59937	0.819326	1.23E-52	-1.69E-46
MS.gene059022	MS.gene59937	0.843289	1.54E-58	-1.69E-46
MS.gene059023	MS.gene59937	0.800749	1.25E-48	-1.69E-46
MS.gene05934	MS.gene59937	0.81064	1.04E-50	-1.69E-46
MS.gene059677	MS.gene59937	0.83823	3.25E-57	-1.69E-46
MS.gene059718	MS.gene59937	0.816191	6.28E-52	-1.69E-46
MS.gene059865	MS.gene59937	0.808043	3.76E-50	-1.69E-46
MS.gene060341	MS.gene59937	0.893324	6.99E-75	-1.69E-46
MS.gene060727	MS.gene59937	0.905043	6.51E-80	-1.69E-46
MS.gene060728	MS.gene59937	0.978037	5.40E-145	-1.69E-46
MS.gene060762	MS.gene59937	0.801929	7.14E-49	-1.69E-46
MS.gene06077	MS.gene59937	0.806754	7.06E-50	-1.69E-46
MS.gene06092	MS.gene59937	0.813283	2.77E-51	-1.69E-46
MS.gene061150	MS.gene59937	0.809063	2.28E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59937.t1	MTR_4g115075	92.350	183	13	1	1	183	1	182	3.73e-101	290
MS.gene59937.t1	MTR_3g106850	31.500	200	103	9	1	183	1	183	3.76e-20	84.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59937.t1	AT4G32280	27.928	222	116	8	1	183	1	217	5.10e-17	76.6
MS.gene59937.t1	AT4G32280	26.471	204	109	7	1	168	1	199	3.52e-13	65.1
MS.gene59937.t1	AT5G25890	31.967	122	77	2	64	183	20	137	4.86e-13	64.3
MS.gene59937.t1	AT1G04550	34.677	124	48	4	93	183	70	193	9.51e-12	62.0
MS.gene59937.t1	AT4G32280	47.368	57	27	1	130	183	119	175	1.75e-11	60.8

Find 44 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTCTTTGAATCATGATAAT+AGG	0.233271	8.2:+13548244	MS.gene59937:CDS
TCTGAGTGGCCAGGAGTTAA+TGG	0.256229	8.2:-13548336	None:intergenic
CAAACATGTCCATCAAAGTT+TGG	0.287284	8.2:-13548744	None:intergenic
TACTCTCAACCAAACTTTGA+TGG	0.301864	8.2:+13548735	MS.gene59937:CDS
TCACAATATGGAGCTTCATT+TGG	0.317095	8.2:+13548158	None:intergenic
GAGAGAAAGAGTGGGAAGGT+TGG	0.357245	8.2:-13548310	None:intergenic
CACAATATGGAGCTTCATTT+GGG	0.385933	8.2:+13548159	None:intergenic
ATCAAGACAAAGAAGGTGAT+TGG	0.388129	8.2:+13548924	MS.gene59937:CDS
TTCTTCCTCCTACAATTAAC+CGG	0.390508	8.2:-13548568	None:intergenic
GATGGCCTCCGGTTAATTGT+AGG	0.427386	8.2:+13548560	MS.gene59937:CDS
GATCACGTGGTCCCCATTGA+TGG	0.439591	8.2:+13548619	MS.gene59937:CDS
CTGAGTGGCCAGGAGTTAAT+GGG	0.451365	8.2:-13548335	None:intergenic
GATGATGAAGAAGCTCTTGT+TGG	0.454535	8.2:+13548538	MS.gene59937:CDS
TAATGGGAGGAGAGAAAGAG+TGG	0.454609	8.2:-13548319	None:intergenic
TCTCTCCTCCCATTAACTCC+TGG	0.458165	8.2:+13548327	MS.gene59937:CDS
AGAAAGAGTGGGAAGGTTGG+TGG	0.462033	8.2:-13548307	None:intergenic
ATGGTTCTCATGATAATCAT+AGG	0.480012	8.2:+13548638	MS.gene59937:CDS
ATTATCATGAGAACCATCAA+TGG	0.488136	8.2:-13548632	None:intergenic
AGCTCTTGTTGGATGGCCTC+CGG	0.493311	8.2:+13548549	MS.gene59937:CDS
TTACTTGGTGATGGTGGTAG+TGG	0.498349	8.2:-13548397	None:intergenic
TGATCAACAACAGTATGAAC+TGG	0.501567	8.2:+13548898	MS.gene59937:CDS
GAATTACGTGAAGGTGAAGA+TGG	0.506755	8.2:+13548660	MS.gene59937:CDS
TTATCATGAGAACCATCAAT+GGG	0.508335	8.2:-13548631	None:intergenic
TATTAATTACTTGGTGATGG+TGG	0.510150	8.2:-13548403	None:intergenic
TAATATTAATTACTTGGTGA+TGG	0.522964	8.2:-13548406	None:intergenic
CGGACGAGAATTATTATAAG+TGG	0.529450	8.2:-13548193	None:intergenic
CTGGTTTATCAAGACAAAGA+AGG	0.557412	8.2:+13548917	MS.gene59937:CDS
GGTTGAGAGTATGAAATGAA+TGG	0.558722	8.2:-13548723	None:intergenic
TCATGATCATCTGAGTGGCC+AGG	0.559365	8.2:-13548345	None:intergenic
TACGTGAAGGTGAAGATGGA+AGG	0.560134	8.2:+13548664	MS.gene59937:CDS
GGAGGAGAGAAAGAGTGGGA+AGG	0.576479	8.2:-13548314	None:intergenic
AATGGGAGGAGAGAAAGAGT+GGG	0.579242	8.2:-13548318	None:intergenic
ATGAAGAAGCTCTTGTTGGA+TGG	0.580018	8.2:+13548542	MS.gene59937:CDS
TATCATGATTCAAAGAGAAG+TGG	0.584987	8.2:-13548239	None:intergenic
GGCCTCCGGTTAATTGTAGG+AGG	0.613414	8.2:+13548563	MS.gene59937:CDS
AGGTGTAGGAATAGCTCGAA+AGG	0.621460	8.2:+13548684	MS.gene59937:CDS
TTAGTAGCAGCAATATCGTA+CGG	0.636596	8.2:-13548213	None:intergenic
AAGGTGAAGATGGAAGGTGT+AGG	0.649720	8.2:+13548670	MS.gene59937:CDS
TTCCTCCTACAATTAACCGG+AGG	0.665193	8.2:-13548565	None:intergenic
TAATCATAGGAATTACGTGA+AGG	0.675805	8.2:+13548651	MS.gene59937:CDS
AGTGGCCAGGAGTTAATGGG+AGG	0.697461	8.2:-13548332	None:intergenic
CATGATCATGATCATCTGAG+TGG	0.721603	8.2:-13548350	None:intergenic
AAATGATGTTGAAGATCACG+TGG	0.746908	8.2:+13548606	MS.gene59937:CDS
TATCATGAGAACCATCAATG+GGG	0.763816	8.2:-13548630	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACTAATAATATTAATTACT+TGG	-	chr8.2:13548415-13548434	None:intergenic	10.0%
!!	GTACATTAATATTATTTATA+TGG	+	chr8.2:13548469-13548488	MS.gene59937:intron	10.0%
!!!	ATTAGTAGTACTAATTATTT+TGG	-	chr8.2:13548811-13548830	None:intergenic	15.0%
!!	AAGTACTAACATTGAAATAT+TGG	-	chr8.2:13548443-13548462	None:intergenic	20.0%
!!!	TAATATTAATTACTTGGTGA+TGG	-	chr8.2:13548409-13548428	None:intergenic	20.0%
!	GTATATATAGTGTATATGCA+CGG	+	chr8.2:13548839-13548858	MS.gene59937:intron	25.0%
!	TCTCTTTGAATCATGATAAT+AGG	+	chr8.2:13548244-13548263	MS.gene59937:CDS	25.0%
!	TTAGTACTACTAATTAGTTG+AGG	+	chr8.2:13548817-13548836	MS.gene59937:intron	25.0%
	ATGGTTCTCATGATAATCAT+AGG	+	chr8.2:13548638-13548657	MS.gene59937:CDS	30.0%
	ATTATCATGAGAACCATCAA+TGG	-	chr8.2:13548635-13548654	None:intergenic	30.0%
	TAATCATAGGAATTACGTGA+AGG	+	chr8.2:13548651-13548670	MS.gene59937:CDS	30.0%
	TATCATGATTCAAAGAGAAG+TGG	-	chr8.2:13548242-13548261	None:intergenic	30.0%
	TCATGTAGTTGGAAATTAAC+AGG	+	chr8.2:13548869-13548888	MS.gene59937:intron	30.0%
	TTATCATGAGAACCATCAAT+GGG	-	chr8.2:13548634-13548653	None:intergenic	30.0%
!!	TATTAATTACTTGGTGATGG+TGG	-	chr8.2:13548406-13548425	None:intergenic	30.0%
	AAATGATGTTGAAGATCACG+TGG	+	chr8.2:13548606-13548625	MS.gene59937:CDS	35.0%
	ACGGCATTAATTCATGTAGT+TGG	+	chr8.2:13548858-13548877	MS.gene59937:intron	35.0%
	CAAACATGTCCATCAAAGTT+TGG	-	chr8.2:13548747-13548766	None:intergenic	35.0%
	CGGACGAGAATTATTATAAG+TGG	-	chr8.2:13548196-13548215	None:intergenic	35.0%
	CTGGTTTATCAAGACAAAGA+AGG	+	chr8.2:13548917-13548936	MS.gene59937:CDS	35.0%
	TACTCTCAACCAAACTTTGA+TGG	+	chr8.2:13548735-13548754	MS.gene59937:CDS	35.0%
	TATCATGAGAACCATCAATG+GGG	-	chr8.2:13548633-13548652	None:intergenic	35.0%
	TGATCAACAACAGTATGAAC+TGG	+	chr8.2:13548898-13548917	MS.gene59937:CDS	35.0%
	TTAGTAGCAGCAATATCGTA+CGG	-	chr8.2:13548216-13548235	None:intergenic	35.0%
	TTCTTCCTCCTACAATTAAC+CGG	-	chr8.2:13548571-13548590	None:intergenic	35.0%
!	ACGTAGTTTTTCTCAACTCT+TGG	+	chr8.2:13548269-13548288	MS.gene59937:CDS	35.0%
!	GGTTGAGAGTATGAAATGAA+TGG	-	chr8.2:13548726-13548745	None:intergenic	35.0%
	ATGAAGAAGCTCTTGTTGGA+TGG	+	chr8.2:13548542-13548561	MS.gene59937:CDS	40.0%
	CATGATCATGATCATCTGAG+TGG	-	chr8.2:13548353-13548372	None:intergenic	40.0%
	GAATTACGTGAAGGTGAAGA+TGG	+	chr8.2:13548660-13548679	MS.gene59937:CDS	40.0%
	GATGATGAAGAAGCTCTTGT+TGG	+	chr8.2:13548538-13548557	MS.gene59937:CDS	40.0%
	AATGGGAGGAGAGAAAGAGT+GGG	-	chr8.2:13548321-13548340	None:intergenic	45.0%
	AGGTGTAGGAATAGCTCGAA+AGG	+	chr8.2:13548684-13548703	MS.gene59937:CDS	45.0%
	TAATGGGAGGAGAGAAAGAG+TGG	-	chr8.2:13548322-13548341	None:intergenic	45.0%
	TACGTGAAGGTGAAGATGGA+AGG	+	chr8.2:13548664-13548683	MS.gene59937:CDS	45.0%
	TTCCTCCTACAATTAACCGG+AGG	-	chr8.2:13548568-13548587	None:intergenic	45.0%
!	AAGGTGAAGATGGAAGGTGT+AGG	+	chr8.2:13548670-13548689	MS.gene59937:CDS	45.0%
!!	TTACTTGGTGATGGTGGTAG+TGG	-	chr8.2:13548400-13548419	None:intergenic	45.0%
!!!	TTAATTTATTTGTTTAATTT+AGG	+	chr8.2:13548506-13548525	MS.gene59937:intron	5.0%
	AGAAAGAGTGGGAAGGTTGG+TGG	-	chr8.2:13548310-13548329	None:intergenic	50.0%
	CTGAGTGGCCAGGAGTTAAT+GGG	-	chr8.2:13548338-13548357	None:intergenic	50.0%
	GAGAGAAAGAGTGGGAAGGT+TGG	-	chr8.2:13548313-13548332	None:intergenic	50.0%
	GATGGCCTCCGGTTAATTGT+AGG	+	chr8.2:13548560-13548579	MS.gene59937:CDS	50.0%
	TCATGATCATCTGAGTGGCC+AGG	-	chr8.2:13548348-13548367	None:intergenic	50.0%
	TCTCTCCTCCCATTAACTCC+TGG	+	chr8.2:13548327-13548346	MS.gene59937:CDS	50.0%
	TCTGAGTGGCCAGGAGTTAA+TGG	-	chr8.2:13548339-13548358	None:intergenic	50.0%
	AGCTCTTGTTGGATGGCCTC+CGG	+	chr8.2:13548549-13548568	MS.gene59937:CDS	55.0%
	AGTGGCCAGGAGTTAATGGG+AGG	-	chr8.2:13548335-13548354	None:intergenic	55.0%
	GATCACGTGGTCCCCATTGA+TGG	+	chr8.2:13548619-13548638	MS.gene59937:CDS	55.0%
	GGAGGAGAGAAAGAGTGGGA+AGG	-	chr8.2:13548317-13548336	None:intergenic	55.0%
	GGCCTCCGGTTAATTGTAGG+AGG	+	chr8.2:13548563-13548582	MS.gene59937:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.2	gene	13548165	13548945	13548165	ID=MS.gene59937
chr8.2	mRNA	13548165	13548945	13548165	ID=MS.gene59937.t1;Parent=MS.gene59937
chr8.2	exon	13548165	13548415	13548165	ID=MS.gene59937.t1.exon1;Parent=MS.gene59937.t1
chr8.2	CDS	13548165	13548415	13548165	ID=cds.MS.gene59937.t1;Parent=MS.gene59937.t1
chr8.2	exon	13548528	13548772	13548528	ID=MS.gene59937.t1.exon2;Parent=MS.gene59937.t1
chr8.2	CDS	13548528	13548772	13548528	ID=cds.MS.gene59937.t1;Parent=MS.gene59937.t1
chr8.2	exon	13548891	13548945	13548891	ID=MS.gene59937.t1.exon3;Parent=MS.gene59937.t1
chr8.2	CDS	13548891	13548945	13548891	ID=cds.MS.gene59937.t1;Parent=MS.gene59937.t1

Gene Sequence

>MS.gene59937

ATGGAGCTTCATTTGGGTCTTGCTCTCTCCACTTATAATAATTCTCGTCCGTACGATATTGCTGCTACTAATAGCCACTTCTCTTTGAATCATGATAATAGGAAACGTAGTTTTTCTCAACTCTTGGAGCATGCAGCAGAAACCACCAACCTTCCCACTCTTTCTCTCCTCCCATTAACTCCTGGCCACTCAGATGATCATGATCATGATGATCAACACTGCTCTCACAGCTCCACTACCACCATCACCAAGTAATTAATATTATTAGTTACTCTCCAATATTTCAATGTTAGTACTTAGTATAGTACATTAATATTATTTATATGGTATATTGAATATTATTAATTTATTTGTTTAATTTAGGAGCAATGAAGATGATGAAGAAGCTCTTGTTGGATGGCCTCCGGTTAATTGTAGGAGGAAGAAAATTCGTTCTAATGAAAATGATGTTGAAGATCACGTGGTCCCCATTGATGGTTCTCATGATAATCATAGGAATTACGTGAAGGTGAAGATGGAAGGTGTAGGAATAGCTCGAAAGGTTAATCTTAGCATGCACCATTCATTTCATACTCTCAACCAAACTTTGATGGACATGTTTGAAAAATGTACGTCTTCTTTATTCAAATTTCTAATTAAATTTCCAAAATAATTAGTACTACTAATTAGTTGAGGTATATATAGTGTATATGCACGGCATTAATTCATGTAGTTGGAAATTAACAGGTGATCATGATCAACAACAGTATGAACTGGTTTATCAAGACAAAGAAGGTGATTG

Protein sequence

>MS.gene59937.t1

MELHLGLALSTYNNSRPYDIAATNSHFSLNHDNRKRSFSQLLEHAAETTNLPTLSLLPLTPGHSDDHDHDDQHCSHSSTTTITKSNEDDEEALVGWPPVNCRRKKIRSNENDVEDHVVPIDGSHDNHRNYVKVKMEGVGIARKVNLSMHHSFHTLNQTLMDMFEKCDHDQQQYELVYQDKEGD