AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene060727


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060727.t1 MTR_4g115075 84.293 191 15 1 9 184 1 191 7.10e-117 330
MS.gene060727.t1 MTR_3g106850 32.673 202 100 8 9 184 1 192 1.65e-23 92.8
MS.gene060727.t1 MTR_1g040675 33.051 118 63 4 77 184 50 161 1.66e-16 73.2
MS.gene060727.t1 MTR_1g040675 33.051 118 63 4 77 184 50 161 6.54e-16 72.4
MS.gene060727.t1 MTR_1g070830 28.889 135 78 3 68 184 46 180 1.99e-14 68.6
MS.gene060727.t1 MTR_1g076270 29.197 137 75 5 70 184 21 157 3.58e-14 67.4
MS.gene060727.t1 MTR_1g093350 29.197 137 75 5 70 184 21 157 3.58e-14 67.4
MS.gene060727.t1 MTR_1g069495 38.202 89 29 2 122 184 147 235 1.92e-13 67.0
MS.gene060727.t1 MTR_4g011880 36.264 91 51 1 101 184 55 145 1.61e-12 63.2
MS.gene060727.t1 MTR_4g124300 36.082 97 51 3 99 184 70 166 2.19e-12 62.8
MS.gene060727.t1 MTR_1g093240 24.658 146 74 2 75 184 58 203 1.34e-11 61.2
MS.gene060727.t1 MTR_1g093240 24.658 146 74 2 75 184 58 203 2.61e-11 60.8
MS.gene060727.t1 MTR_1g070520 27.568 185 85 7 42 184 35 212 2.73e-11 60.8
MS.gene060727.t1 MTR_1g093240 24.658 146 74 2 75 184 58 203 3.06e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene060727.t1 AT4G32280 28.571 231 105 8 9 184 1 226 1.34e-20 86.3
MS.gene060727.t1 AT4G32280 30.000 190 113 6 9 184 1 184 5.48e-18 78.2
MS.gene060727.t1 AT5G25890 32.283 127 72 2 72 184 20 146 6.82e-17 74.7
MS.gene060727.t1 AT3G23030 30.303 132 73 5 69 184 21 149 6.82e-16 72.0
MS.gene060727.t1 AT3G23030 30.303 132 73 5 69 184 108 236 1.60e-15 72.8
MS.gene060727.t1 AT5G43700 33.036 112 61 2 87 184 49 160 3.80e-15 70.5
MS.gene060727.t1 AT2G33310 26.797 153 57 3 82 184 63 210 1.27e-14 70.1
MS.gene060727.t1 AT2G33310 28.358 134 46 2 101 184 76 209 1.41e-14 69.7
MS.gene060727.t1 AT2G33310 28.358 134 46 2 101 184 76 209 1.41e-14 69.7
MS.gene060727.t1 AT3G17600 32.692 104 58 3 93 184 37 140 1.49e-13 65.5
MS.gene060727.t1 AT3G15540 37.113 97 48 2 101 184 71 167 1.77e-13 66.2
MS.gene060727.t1 AT1G04550 30.075 133 44 4 101 184 70 202 1.84e-13 66.6
MS.gene060727.t1 AT1G15580 38.462 91 48 4 101 184 54 143 4.23e-13 64.3
MS.gene060727.t1 AT1G52830 35.106 94 48 4 102 184 72 163 1.63e-12 63.2
MS.gene060727.t1 AT4G14560 34.783 92 51 2 101 184 56 146 9.49e-11 58.2

Find 41 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTTTGAATCATGATAAT+AGG 0.233271 8.3:-12111685 MS.gene060727:CDS
TCTGAGTGGCCAGGAGTTAA+TGG 0.256229 8.3:+12111593 None:intergenic
CAAACATGTCCATCAAAGTT+TGG 0.287284 8.3:+12111186 None:intergenic
TACTCTCAACCAAACTTTGA+TGG 0.301864 8.3:-12111195 MS.gene060727:CDS
TCACAATATGGAGCTTCATT+TGG 0.317095 8.3:-12111771 MS.gene060727:CDS
CTCTCTACACAATCACAATA+TGG 0.343546 8.3:-12111783 MS.gene060727:CDS
GAGAGAAAGAGTGGGAAGGT+TGG 0.357245 8.3:+12111619 None:intergenic
CACAATATGGAGCTTCATTT+GGG 0.385933 8.3:-12111770 MS.gene060727:CDS
ATCAAGACAAAGAAGGTGAT+TGG 0.388129 8.3:-12111007 MS.gene060727:CDS
TTCTTCCTCCTACAATTAAC+CGG 0.390508 8.3:+12111361 None:intergenic
TTCTTGCTCAAGATATTTCA+TGG 0.399762 8.3:-12110983 MS.gene060727:CDS
GATGGCCTCCGGTTAATTGT+AGG 0.427386 8.3:-12111369 MS.gene060727:CDS
CTGAGTGGCCAGGAGTTAAT+GGG 0.451365 8.3:+12111594 None:intergenic
GATGATGAAGAAGCTCTTGT+TGG 0.454535 8.3:-12111391 MS.gene060727:CDS
TAATGGGAGGAGAGAAAGAG+TGG 0.454609 8.3:+12111610 None:intergenic
TCTCTCCTCCCATTAACTCC+TGG 0.458165 8.3:-12111602 MS.gene060727:CDS
AGAAAGAGTGGGAAGGTTGG+TGG 0.462033 8.3:+12111622 None:intergenic
AGCTCTTGTTGGATGGCCTC+CGG 0.493311 8.3:-12111380 MS.gene060727:CDS
TTACTTGGTGATGGTGGTAG+TGG 0.498349 8.3:+12111532 None:intergenic
TGATCAACAACAGTATGAAC+TGG 0.501567 8.3:-12111033 MS.gene060727:CDS
GAATTACGTGAAGGTGAAGA+TGG 0.506755 8.3:-12111270 MS.gene060727:CDS
TATTAATTACTTGGTGATGG+TGG 0.510150 8.3:+12111526 None:intergenic
TAATATTAATTACTTGGTGA+TGG 0.522964 8.3:+12111523 None:intergenic
CGGACGAGAATTATTATAAG+TGG 0.529450 8.3:+12111736 None:intergenic
CTGGTTTATCAAGACAAAGA+AGG 0.557412 8.3:-12111014 MS.gene060727:CDS
GGTTGAGAGTATGAAATGAA+TGG 0.558722 8.3:+12111207 None:intergenic
TCATGATCATCTGAGTGGCC+AGG 0.559365 8.3:+12111584 None:intergenic
TACGTGAAGGTGAAGATGGA+AGG 0.560134 8.3:-12111266 MS.gene060727:CDS
GGAGGAGAGAAAGAGTGGGA+AGG 0.576479 8.3:+12111615 None:intergenic
AATGGGAGGAGAGAAAGAGT+GGG 0.579242 8.3:+12111611 None:intergenic
ATGAAGAAGCTCTTGTTGGA+TGG 0.580018 8.3:-12111387 MS.gene060727:CDS
TATCATGATTCAAAGAGAAG+TGG 0.584987 8.3:+12111690 None:intergenic
GGCCTCCGGTTAATTGTAGG+AGG 0.613414 8.3:-12111366 MS.gene060727:CDS
AGGTGTAGGAATAGCTCGAA+AGG 0.621460 8.3:-12111246 MS.gene060727:CDS
TTAGTAGCAGCAATATCGTA+CGG 0.636596 8.3:+12111716 None:intergenic
AAGGTGAAGATGGAAGGTGT+AGG 0.649720 8.3:-12111260 MS.gene060727:CDS
TTCCTCCTACAATTAACCGG+AGG 0.665193 8.3:+12111364 None:intergenic
TAATCATAGGAATTACGTGA+AGG 0.675805 8.3:-12111279 MS.gene060727:intron
AGTGGCCAGGAGTTAATGGG+AGG 0.697461 8.3:+12111597 None:intergenic
TTGCTCAAGATATTTCATGG+AGG 0.720826 8.3:-12110980 None:intergenic
CATGATCATGATCATCTGAG+TGG 0.721603 8.3:+12111579 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTAATAATATTAATTACT+TGG + chr8.3:12111255-12111274 None:intergenic 10.0%
!! GTACATTAATATTATTTATA+TGG - chr8.3:12111309-12111328 MS.gene060727:intron 10.0%
!!! ATTAGTAGTACTAATTATTT+TGG + chr8.3:12111649-12111668 None:intergenic 15.0%
!! AAGTACTAACATTGAAATAT+TGG + chr8.3:12111283-12111302 None:intergenic 20.0%
!!! TAATATTAATTACTTGGTGA+TGG + chr8.3:12111249-12111268 None:intergenic 20.0%
! GTATATATAGTGTATATGCA+CGG - chr8.3:12111677-12111696 MS.gene060727:CDS 25.0%
! TCTCTTTGAATCATGATAAT+AGG - chr8.3:12111084-12111103 MS.gene060727:intron 25.0%
! TTAGTACTACTAATTAGTTG+AGG - chr8.3:12111655-12111674 MS.gene060727:CDS 25.0%
ATGGTTCTCATGATAATCAT+AGG - chr8.3:12111477-12111496 MS.gene060727:intron 30.0%
ATTATCATGAGAACCATCAA+TGG + chr8.3:12111474-12111493 None:intergenic 30.0%
TAATCATAGGAATTACGTGA+AGG - chr8.3:12111490-12111509 MS.gene060727:intron 30.0%
TATCATGATTCAAAGAGAAG+TGG + chr8.3:12111082-12111101 None:intergenic 30.0%
TCATGTAGTTGGAAATTAAC+AGG - chr8.3:12111707-12111726 MS.gene060727:CDS 30.0%
TTATCATGAGAACCATCAAT+GGG + chr8.3:12111473-12111492 None:intergenic 30.0%
TTCTTGCTCAAGATATTTCA+TGG - chr8.3:12111786-12111805 MS.gene060727:CDS 30.0%
!! TATTAATTACTTGGTGATGG+TGG + chr8.3:12111246-12111265 None:intergenic 30.0%
AAATGATGTTGAAGATCACG+TGG - chr8.3:12111445-12111464 MS.gene060727:intron 35.0%
ACGGCATTAATTCATGTAGT+TGG - chr8.3:12111696-12111715 MS.gene060727:CDS 35.0%
ATCAAGACAAAGAAGGTGAT+TGG - chr8.3:12111762-12111781 MS.gene060727:CDS 35.0%
CAAACATGTCCATCAAAGTT+TGG + chr8.3:12111586-12111605 None:intergenic 35.0%
CACAATATGGAGCTTCATTT+GGG - chr8.3:12110999-12111018 MS.gene060727:CDS 35.0%
CGGACGAGAATTATTATAAG+TGG + chr8.3:12111036-12111055 None:intergenic 35.0%
CTCTCTACACAATCACAATA+TGG - chr8.3:12110986-12111005 MS.gene060727:CDS 35.0%
CTGGTTTATCAAGACAAAGA+AGG - chr8.3:12111755-12111774 MS.gene060727:CDS 35.0%
TACTCTCAACCAAACTTTGA+TGG - chr8.3:12111574-12111593 MS.gene060727:CDS 35.0%
TATCATGAGAACCATCAATG+GGG + chr8.3:12111472-12111491 None:intergenic 35.0%
TCACAATATGGAGCTTCATT+TGG - chr8.3:12110998-12111017 MS.gene060727:CDS 35.0%
TGATCAACAACAGTATGAAC+TGG - chr8.3:12111736-12111755 MS.gene060727:CDS 35.0%
TTAGTAGCAGCAATATCGTA+CGG + chr8.3:12111056-12111075 None:intergenic 35.0%
TTCTTCCTCCTACAATTAAC+CGG + chr8.3:12111411-12111430 None:intergenic 35.0%
! ACGTAGTTTTTCTCAACTCT+TGG - chr8.3:12111109-12111128 MS.gene060727:intron 35.0%
! GGTTGAGAGTATGAAATGAA+TGG + chr8.3:12111565-12111584 None:intergenic 35.0%
ATGAAGAAGCTCTTGTTGGA+TGG - chr8.3:12111382-12111401 MS.gene060727:CDS 40.0%
CATGATCATGATCATCTGAG+TGG + chr8.3:12111193-12111212 None:intergenic 40.0%
GAATTACGTGAAGGTGAAGA+TGG - chr8.3:12111499-12111518 MS.gene060727:intron 40.0%
GATGATGAAGAAGCTCTTGT+TGG - chr8.3:12111378-12111397 MS.gene060727:CDS 40.0%
AATGGGAGGAGAGAAAGAGT+GGG + chr8.3:12111161-12111180 None:intergenic 45.0%
AGGTGTAGGAATAGCTCGAA+AGG - chr8.3:12111523-12111542 MS.gene060727:intron 45.0%
TAATGGGAGGAGAGAAAGAG+TGG + chr8.3:12111162-12111181 None:intergenic 45.0%
TACGTGAAGGTGAAGATGGA+AGG - chr8.3:12111503-12111522 MS.gene060727:intron 45.0%
TTCCTCCTACAATTAACCGG+AGG + chr8.3:12111408-12111427 None:intergenic 45.0%
! AAGGTGAAGATGGAAGGTGT+AGG - chr8.3:12111509-12111528 MS.gene060727:intron 45.0%
!! TTACTTGGTGATGGTGGTAG+TGG + chr8.3:12111240-12111259 None:intergenic 45.0%
!!! TTAATTTATTTGTTTAATTT+AGG - chr8.3:12111346-12111365 MS.gene060727:CDS 5.0%
AGAAAGAGTGGGAAGGTTGG+TGG + chr8.3:12111150-12111169 None:intergenic 50.0%
CTGAGTGGCCAGGAGTTAAT+GGG + chr8.3:12111178-12111197 None:intergenic 50.0%
GAGAGAAAGAGTGGGAAGGT+TGG + chr8.3:12111153-12111172 None:intergenic 50.0%
GATGGCCTCCGGTTAATTGT+AGG - chr8.3:12111400-12111419 MS.gene060727:CDS 50.0%
TCATGATCATCTGAGTGGCC+AGG + chr8.3:12111188-12111207 None:intergenic 50.0%
TCTCTCCTCCCATTAACTCC+TGG - chr8.3:12111167-12111186 MS.gene060727:intron 50.0%
TCTGAGTGGCCAGGAGTTAA+TGG + chr8.3:12111179-12111198 None:intergenic 50.0%
AGCTCTTGTTGGATGGCCTC+CGG - chr8.3:12111389-12111408 MS.gene060727:CDS 55.0%
AGTGGCCAGGAGTTAATGGG+AGG + chr8.3:12111175-12111194 None:intergenic 55.0%
GATCACGTGGTCCCCATTGA+TGG - chr8.3:12111458-12111477 MS.gene060727:intron 55.0%
GGAGGAGAGAAAGAGTGGGA+AGG + chr8.3:12111157-12111176 None:intergenic 55.0%
GGCCTCCGGTTAATTGTAGG+AGG - chr8.3:12111403-12111422 MS.gene060727:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 12110981 12111810 12110981 ID=MS.gene060727
chr8.3 mRNA 12110981 12111810 12110981 ID=MS.gene060727.t1;Parent=MS.gene060727
chr8.3 exon 12111536 12111810 12111536 ID=MS.gene060727.t1.exon1;Parent=MS.gene060727.t1
chr8.3 CDS 12111536 12111810 12111536 ID=cds.MS.gene060727.t1;Parent=MS.gene060727.t1
chr8.3 exon 12111340 12111423 12111340 ID=MS.gene060727.t1.exon2;Parent=MS.gene060727.t1
chr8.3 CDS 12111340 12111423 12111340 ID=cds.MS.gene060727.t1;Parent=MS.gene060727.t1
chr8.3 exon 12111180 12111292 12111180 ID=MS.gene060727.t1.exon3;Parent=MS.gene060727.t1
chr8.3 CDS 12111180 12111292 12111180 ID=cds.MS.gene060727.t1;Parent=MS.gene060727.t1
chr8.3 exon 12110981 12111062 12110981 ID=MS.gene060727.t1.exon4;Parent=MS.gene060727.t1
chr8.3 CDS 12110981 12111062 12110981 ID=cds.MS.gene060727.t1;Parent=MS.gene060727.t1
Gene Sequence

>MS.gene060727

CTTATCTCTCTACACAATCACAATATGGAGCTTCATTTGGGTCTTGCTCTCTCCACTTATAATAATTCTCGTCCGTACGATATTGCTGCTACTAATAGCCACTTCTCTTTGAATCATGATAATAGGAAACGTAGTTTTTCTCAACTCTTGGAGCATGCAGCAGAAACCACCAACCTTCCCACTCTTTCTCTCCTCCCATTAACTCCTGGCCACTCAGATGATCATGATCATGATGATCAACACTGCTCTCACAGCTCCACTACCACCATCACCAAGTAATTAATATTATTAGTTACTCTCCAATATTTCAATGTTAGTACTTAGTATAGTACATTAATATTATTTATATGGTATATTGAATATTATTAATTTATTTGTTTAATTTAGGAGCAATGAAGATGATGAAGAAGCTCTTGTTGGATGGCCTCCGGTTAATTGTAGGAGGAAGAAAATTCGTTCTAATGAAATGATGTTGAAGATCACGTGGTCCCCATTGATGGTTCTCATGATAATCATAGGAATTACGTGAAGGTGAAGATGGAAGGTGTAGGAATAGCTCGAAAGGTTAATCTTAGCATGCACCATTCATTTCATACTCTCAACCAAACTTTGATGGACATGTTTGAAAAATGTACGTCTTCTTTATTCAAATTTCTAATTAATTTCCAAAATAATTAGTACTACTAATTAGTTGAGGTATATATAGTGTATATGCACGGCATTAATTCATGTAGTTGGAAATTAACAGGTGATCATGATCAACAACAGTATGAACTGGTTTATCAAGACAAAGAAGGTGATTGGCTTCTTGCTCAAGATATTTCATGGAG

Protein sequence

>MS.gene060727.t1

LISLHNHNMELHLGLALSTYNNSRPYDIAATNSHFSLNHDNRKRSFSQLLEHAAETTNLPTLSLLPLTPGHSDDHDHDDQHCSHSSTTTITKSNEDDEEALVGWPPVNCRRKKIRSNEMMNYVKVKMEGVGIARKVNLSMHHSFHTLNQTLMDMFEKCDHDQQQYELVYQDKEGDWLLAQDISW