Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74960.t1 | KEH44330.1 | 94.2 | 588 | 31 | 3 | 1 | 586 | 51 | 637 | 0.00E+00 | 1079.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74960.t1 | Q6F6B5 | 41.7 | 578 | 306 | 10 | 20 | 586 | 69 | 626 | 2.8e-110 | 401.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74960.t1 | A0A072W244 | 94.2 | 588 | 31 | 3 | 1 | 586 | 51 | 637 | 0.0e+00 | 1079.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049151 | MS.gene74960 | 0.803621 | 3.19E-49 | -1.69E-46 |
| MS.gene049297 | MS.gene74960 | 0.868074 | 8.55E-66 | -1.69E-46 |
| MS.gene049378 | MS.gene74960 | 0.822116 | 2.81E-53 | -1.69E-46 |
| MS.gene049382 | MS.gene74960 | 0.801425 | 9.06E-49 | -1.69E-46 |
| MS.gene049599 | MS.gene74960 | -0.814703 | 1.35E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene74960 | 0.858444 | 8.21E-63 | -1.69E-46 |
| MS.gene049915 | MS.gene74960 | 0.820237 | 7.63E-53 | -1.69E-46 |
| MS.gene050226 | MS.gene74960 | 0.834326 | 3.20E-56 | -1.69E-46 |
| MS.gene050227 | MS.gene74960 | 0.83775 | 4.32E-57 | -1.69E-46 |
| MS.gene050228 | MS.gene74960 | 0.830133 | 3.49E-55 | -1.69E-46 |
| MS.gene050301 | MS.gene74960 | 0.83112 | 2.00E-55 | -1.69E-46 |
| MS.gene050319 | MS.gene74960 | -0.843042 | 1.79E-58 | -1.69E-46 |
| MS.gene050330 | MS.gene74960 | -0.822739 | 2.02E-53 | -1.69E-46 |
| MS.gene050558 | MS.gene74960 | 0.816817 | 4.55E-52 | -1.69E-46 |
| MS.gene050623 | MS.gene74960 | -0.83614 | 1.11E-56 | -1.69E-46 |
| MS.gene050642 | MS.gene74960 | 0.841999 | 3.38E-58 | -1.69E-46 |
| MS.gene050820 | MS.gene74960 | 0.809497 | 1.84E-50 | -1.69E-46 |
| MS.gene05119 | MS.gene74960 | -0.81757 | 3.08E-52 | -1.69E-46 |
| MS.gene051471 | MS.gene74960 | 0.811991 | 5.31E-51 | -1.69E-46 |
| MS.gene051662 | MS.gene74960 | 0.852148 | 5.58E-61 | -1.69E-46 |
| MS.gene051663 | MS.gene74960 | 0.847539 | 1.08E-59 | -1.69E-46 |
| MS.gene051860 | MS.gene74960 | 0.82927 | 5.65E-55 | -1.69E-46 |
| MS.gene051983 | MS.gene74960 | 0.833508 | 5.12E-56 | -1.69E-46 |
| MS.gene052176 | MS.gene74960 | 0.806677 | 7.32E-50 | -1.69E-46 |
| MS.gene052178 | MS.gene74960 | 0.810053 | 1.40E-50 | -1.69E-46 |
| MS.gene052256 | MS.gene74960 | 0.809799 | 1.58E-50 | -1.69E-46 |
| MS.gene052286 | MS.gene74960 | 0.807836 | 4.16E-50 | -1.69E-46 |
| MS.gene052302 | MS.gene74960 | 0.825534 | 4.45E-54 | -1.69E-46 |
| MS.gene052309 | MS.gene74960 | -0.821338 | 4.26E-53 | -1.69E-46 |
| MS.gene052376 | MS.gene74960 | 0.829501 | 4.97E-55 | -1.69E-46 |
| MS.gene052600 | MS.gene74960 | 0.818659 | 1.75E-52 | -1.69E-46 |
| MS.gene052856 | MS.gene74960 | 0.801075 | 1.07E-48 | -1.69E-46 |
| MS.gene052858 | MS.gene74960 | 0.804908 | 1.72E-49 | -1.69E-46 |
| MS.gene052860 | MS.gene74960 | 0.801261 | 9.79E-49 | -1.69E-46 |
| MS.gene053557 | MS.gene74960 | 0.802468 | 5.53E-49 | -1.69E-46 |
| MS.gene053630 | MS.gene74960 | 0.812886 | 3.38E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene74960 | 0.87605 | 1.90E-68 | -1.69E-46 |
| MS.gene054184 | MS.gene74960 | 0.817289 | 3.56E-52 | -1.69E-46 |
| MS.gene054185 | MS.gene74960 | 0.826243 | 3.02E-54 | -1.69E-46 |
| MS.gene054379 | MS.gene74960 | 0.808169 | 3.53E-50 | -1.69E-46 |
| MS.gene054473 | MS.gene74960 | 0.813201 | 2.88E-51 | -1.69E-46 |
| MS.gene054667 | MS.gene74960 | 0.815103 | 1.10E-51 | -1.69E-46 |
| MS.gene054930 | MS.gene74960 | 0.851718 | 7.40E-61 | -1.69E-46 |
| MS.gene054942 | MS.gene74960 | 0.847862 | 8.84E-60 | -1.69E-46 |
| MS.gene054983 | MS.gene74960 | 0.814777 | 1.30E-51 | -1.69E-46 |
| MS.gene055133 | MS.gene74960 | 0.807271 | 5.48E-50 | -1.69E-46 |
| MS.gene055277 | MS.gene74960 | 0.807496 | 4.91E-50 | -1.69E-46 |
| MS.gene055422 | MS.gene74960 | 0.857376 | 1.70E-62 | -1.69E-46 |
| MS.gene055428 | MS.gene74960 | 0.834865 | 2.34E-56 | -1.69E-46 |
| MS.gene055604 | MS.gene74960 | 0.803301 | 3.72E-49 | -1.69E-46 |
| MS.gene056069 | MS.gene74960 | -0.860899 | 1.50E-63 | -1.69E-46 |
| MS.gene056314 | MS.gene74960 | 0.800243 | 1.58E-48 | -1.69E-46 |
| MS.gene056612 | MS.gene74960 | 0.806661 | 7.38E-50 | -1.69E-46 |
| MS.gene056979 | MS.gene74960 | 0.828335 | 9.52E-55 | -1.69E-46 |
| MS.gene057121 | MS.gene74960 | 0.841452 | 4.71E-58 | -1.69E-46 |
| MS.gene057245 | MS.gene74960 | 0.823014 | 1.74E-53 | -1.69E-46 |
| MS.gene057260 | MS.gene74960 | 0.821491 | 3.93E-53 | -1.69E-46 |
| MS.gene057439 | MS.gene74960 | 0.822272 | 2.59E-53 | -1.69E-46 |
| MS.gene057754 | MS.gene74960 | 0.820625 | 6.22E-53 | -1.69E-46 |
| MS.gene058002 | MS.gene74960 | 0.83112 | 2.00E-55 | -1.69E-46 |
| MS.gene05811 | MS.gene74960 | 0.829965 | 3.83E-55 | -1.69E-46 |
| MS.gene058142 | MS.gene74960 | 0.833726 | 4.52E-56 | -1.69E-46 |
| MS.gene058182 | MS.gene74960 | 0.800269 | 1.56E-48 | -1.69E-46 |
| MS.gene058201 | MS.gene74960 | 0.817313 | 3.52E-52 | -1.69E-46 |
| MS.gene058399 | MS.gene74960 | 0.809756 | 1.62E-50 | -1.69E-46 |
| MS.gene058710 | MS.gene74960 | 0.816365 | 5.74E-52 | -1.69E-46 |
| MS.gene058749 | MS.gene74960 | 0.868496 | 6.25E-66 | -1.69E-46 |
| MS.gene058833 | MS.gene74960 | 0.821167 | 4.66E-53 | -1.69E-46 |
| MS.gene058845 | MS.gene74960 | 0.827566 | 1.46E-54 | -1.69E-46 |
| MS.gene059285 | MS.gene74960 | 0.813263 | 2.80E-51 | -1.69E-46 |
| MS.gene059518 | MS.gene74960 | 0.813418 | 2.59E-51 | -1.69E-46 |
| MS.gene05984 | MS.gene74960 | 0.860134 | 2.55E-63 | -1.69E-46 |
| MS.gene059894 | MS.gene74960 | 0.831534 | 1.58E-55 | -1.69E-46 |
| MS.gene059899 | MS.gene74960 | 0.815391 | 9.47E-52 | -1.69E-46 |
| MS.gene060404 | MS.gene74960 | 0.803125 | 4.05E-49 | -1.69E-46 |
| MS.gene060476 | MS.gene74960 | 0.877737 | 4.94E-69 | -1.69E-46 |
| MS.gene061049 | MS.gene74960 | 0.843621 | 1.25E-58 | -1.69E-46 |
| MS.gene061113 | MS.gene74960 | 0.802981 | 4.33E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74960.t1 | MTR_1g112280 | 94.978 | 697 | 32 | 3 | 1 | 695 | 51 | 746 | 0.0 | 1340 |
| MS.gene74960.t1 | MTR_1g112280 | 94.577 | 627 | 31 | 3 | 1 | 625 | 51 | 676 | 0.0 | 1188 |
| MS.gene74960.t1 | MTR_8g080340 | 32.090 | 134 | 91 | 0 | 559 | 692 | 16 | 149 | 1.19e-18 | 84.3 |
| MS.gene74960.t1 | MTR_1g050740 | 27.907 | 215 | 111 | 5 | 501 | 690 | 17 | 212 | 1.37e-13 | 74.7 |
| MS.gene74960.t1 | MTR_5g459450 | 34.375 | 128 | 82 | 2 | 564 | 690 | 94 | 220 | 1.64e-13 | 74.7 |
| MS.gene74960.t1 | MTR_8g080310 | 30.894 | 123 | 85 | 0 | 567 | 689 | 15 | 137 | 1.66e-13 | 69.3 |
| MS.gene74960.t1 | MTR_3g073100 | 35.200 | 125 | 79 | 2 | 567 | 690 | 86 | 209 | 2.03e-13 | 74.3 |
| MS.gene74960.t1 | MTR_8g095170 | 31.429 | 140 | 71 | 3 | 576 | 691 | 45 | 183 | 3.98e-12 | 70.1 |
| MS.gene74960.t1 | MTR_1g062740 | 26.490 | 151 | 88 | 1 | 564 | 691 | 53 | 203 | 5.19e-12 | 69.7 |
| MS.gene74960.t1 | MTR_8g095170 | 31.429 | 140 | 71 | 3 | 576 | 691 | 45 | 183 | 6.74e-12 | 68.9 |
| MS.gene74960.t1 | MTR_7g451440 | 30.597 | 134 | 93 | 0 | 558 | 691 | 234 | 367 | 7.68e-12 | 68.6 |
| MS.gene74960.t1 | MTR_8g088930 | 29.134 | 127 | 90 | 0 | 564 | 690 | 61 | 187 | 1.01e-11 | 68.9 |
| MS.gene74960.t1 | MTR_8g088930 | 30.822 | 146 | 91 | 3 | 507 | 652 | 60 | 195 | 8.46e-11 | 65.9 |
| MS.gene74960.t1 | MTR_8g088930 | 29.134 | 127 | 90 | 0 | 564 | 690 | 61 | 187 | 1.02e-11 | 68.9 |
| MS.gene74960.t1 | MTR_8g088930 | 30.822 | 146 | 91 | 3 | 507 | 652 | 60 | 195 | 8.38e-11 | 65.9 |
| MS.gene74960.t1 | MTR_8g007500 | 32.836 | 134 | 90 | 0 | 558 | 691 | 239 | 372 | 1.28e-11 | 67.8 |
| MS.gene74960.t1 | MTR_8g007500 | 28.022 | 182 | 104 | 1 | 535 | 689 | 166 | 347 | 7.30e-11 | 65.5 |
| MS.gene74960.t1 | MTR_8g088930 | 29.134 | 127 | 90 | 0 | 564 | 690 | 61 | 187 | 2.79e-11 | 67.4 |
| MS.gene74960.t1 | MTR_8g088930 | 29.134 | 127 | 90 | 0 | 564 | 690 | 61 | 187 | 2.79e-11 | 67.4 |
| MS.gene74960.t1 | MTR_8g039100 | 33.333 | 126 | 84 | 0 | 564 | 689 | 78 | 203 | 3.06e-11 | 67.4 |
| MS.gene74960.t1 | MTR_7g089950 | 32.308 | 130 | 82 | 4 | 564 | 690 | 96 | 222 | 3.78e-11 | 67.0 |
| MS.gene74960.t1 | MTR_5g058920 | 31.387 | 137 | 94 | 0 | 558 | 694 | 239 | 375 | 4.49e-11 | 66.2 |
| MS.gene74960.t1 | MTR_4g107460 | 31.013 | 158 | 108 | 1 | 530 | 686 | 23 | 180 | 4.86e-11 | 65.5 |
| MS.gene74960.t1 | MTR_3g097260 | 30.625 | 160 | 86 | 4 | 555 | 690 | 48 | 206 | 5.16e-11 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74960.t1 | AT1G75010 | 46.957 | 690 | 335 | 10 | 20 | 698 | 69 | 738 | 0.0 | 619 |
| MS.gene74960.t1 | AT1G60890 | 35.433 | 127 | 82 | 0 | 564 | 690 | 71 | 197 | 2.66e-16 | 83.6 |
| MS.gene74960.t1 | AT1G60890 | 35.433 | 127 | 82 | 0 | 564 | 690 | 59 | 185 | 2.86e-16 | 83.6 |
| MS.gene74960.t1 | AT2G26420 | 30.055 | 183 | 124 | 2 | 510 | 691 | 3 | 182 | 1.44e-14 | 77.8 |
| MS.gene74960.t1 | AT2G26420 | 30.055 | 183 | 124 | 2 | 510 | 691 | 3 | 182 | 1.44e-14 | 77.8 |
| MS.gene74960.t1 | AT2G26420 | 30.055 | 183 | 124 | 2 | 510 | 691 | 3 | 182 | 2.10e-14 | 77.4 |
| MS.gene74960.t1 | AT1G21980 | 27.027 | 185 | 106 | 3 | 535 | 690 | 43 | 227 | 5.21e-14 | 76.3 |
| MS.gene74960.t1 | AT3G07960 | 30.667 | 150 | 81 | 2 | 564 | 690 | 29 | 178 | 4.46e-13 | 73.2 |
| MS.gene74960.t1 | AT1G10900 | 30.709 | 127 | 88 | 0 | 564 | 690 | 59 | 185 | 5.30e-13 | 73.2 |
| MS.gene74960.t1 | AT1G10900 | 30.709 | 127 | 88 | 0 | 564 | 690 | 59 | 185 | 5.30e-13 | 73.2 |
| MS.gene74960.t1 | AT1G10900 | 30.709 | 127 | 88 | 0 | 564 | 690 | 59 | 185 | 5.30e-13 | 73.2 |
| MS.gene74960.t1 | AT1G77740 | 30.573 | 157 | 104 | 2 | 538 | 691 | 52 | 206 | 7.68e-13 | 72.4 |
| MS.gene74960.t1 | AT1G77740 | 30.573 | 157 | 104 | 2 | 538 | 691 | 52 | 206 | 7.68e-13 | 72.4 |
| MS.gene74960.t1 | AT4G17080 | 29.851 | 134 | 94 | 0 | 558 | 691 | 299 | 432 | 8.83e-13 | 71.6 |
| MS.gene74960.t1 | AT3G07960 | 30.667 | 150 | 81 | 2 | 564 | 690 | 29 | 178 | 8.83e-13 | 72.0 |
| MS.gene74960.t1 | AT3G07960 | 30.667 | 150 | 81 | 2 | 564 | 690 | 29 | 178 | 9.85e-13 | 72.0 |
| MS.gene74960.t1 | AT3G07960 | 30.667 | 150 | 81 | 2 | 564 | 690 | 29 | 178 | 9.85e-13 | 72.0 |
| MS.gene74960.t1 | AT2G35170 | 29.851 | 134 | 94 | 0 | 558 | 691 | 268 | 401 | 6.61e-12 | 68.9 |
Find 167 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCATCAGCTTCCCTTTCT+TGG | 0.160785 | 1.3:-76146685 | None:intergenic |
| TGAATAGGTACACTGAAATT+TGG | 0.182648 | 1.3:+76149431 | MS.gene74960:intron |
| TATATTTGATTGTCCTTTCC+TGG | 0.194395 | 1.3:+76144608 | MS.gene74960:CDS |
| GAGAATACTAATTTACTTAT+TGG | 0.199862 | 1.3:+76142920 | MS.gene74960:CDS |
| GAAATATCTATGATGGTTTA+TGG | 0.235774 | 1.3:+76147536 | MS.gene74960:CDS |
| ATGCATATGACTGCACTATT+TGG | 0.239687 | 1.3:-76144893 | None:intergenic |
| AGGAAAATGCAGGGCAAGTT+TGG | 0.247568 | 1.3:-76146099 | None:intergenic |
| TTTAATAATTTCTGAATTGC+AGG | 0.270512 | 1.3:-76144385 | None:intergenic |
| GACAGAACACCGTGCTTCTT+TGG | 0.282395 | 1.3:-76146890 | None:intergenic |
| CTTAACCCTTCAAAGCTAAA+AGG | 0.285883 | 1.3:-76142557 | None:intergenic |
| AAATCTGTCATCTATAATAC+TGG | 0.287616 | 1.3:-76146243 | None:intergenic |
| ATCGGTGGTTTGCAAACTTC+TGG | 0.294923 | 1.3:+76147935 | MS.gene74960:CDS |
| AAAATTACCTTCTTCATTAA+CGG | 0.298913 | 1.3:-76146485 | None:intergenic |
| AAGAAGCCAGAATTGGATTT+GGG | 0.299412 | 1.3:+76144550 | MS.gene74960:CDS |
| AAGTCTGGTGATGCCTTCTT+TGG | 0.305312 | 1.3:+76147994 | MS.gene74960:CDS |
| AAAGAAGCCAGAATTGGATT+TGG | 0.312541 | 1.3:+76144549 | MS.gene74960:CDS |
| TTTCCTGGGTGTTTCTTTGA+AGG | 0.320867 | 1.3:+76144623 | MS.gene74960:CDS |
| AGTGGGGCTTTGACAATTCC+TGG | 0.322290 | 1.3:-76142045 | None:intergenic |
| TAGGTGGCTGGTGCAGGATA+TGG | 0.340990 | 1.3:+76142455 | MS.gene74960:intron |
| CGCAGAGAAGACAGCTGAAA+TGG | 0.344238 | 1.3:-76141613 | None:intergenic |
| ATCATCAGCTTCCCTTTCTT+GGG | 0.346872 | 1.3:-76146684 | None:intergenic |
| TTCTTGAAATAGAATGTTCC+TGG | 0.349806 | 1.3:-76147580 | None:intergenic |
| GCAGCTTGGAAACTGGAATC+TGG | 0.352297 | 1.3:+76146631 | MS.gene74960:CDS |
| TGTTGTGGAAATACAATATA+GGG | 0.352525 | 1.3:+76147319 | MS.gene74960:CDS |
| CTAGGGAGCAGCTTGGAAAC+TGG | 0.352578 | 1.3:+76146624 | MS.gene74960:CDS |
| CTTCCTTAGGTGGCTGGTGC+AGG | 0.355054 | 1.3:+76142449 | MS.gene74960:intron |
| AGCAGCCCCAAATCCAATTC+TGG | 0.372964 | 1.3:-76144556 | None:intergenic |
| CTGTTGTGGAAATACAATAT+AGG | 0.375868 | 1.3:+76147318 | MS.gene74960:CDS |
| TTCTCAGGTAAAAGCTTTGA+TGG | 0.377982 | 1.3:+76142886 | MS.gene74960:intron |
| TCTTCATTAACGGATTCATA+AGG | 0.378330 | 1.3:-76146475 | None:intergenic |
| GCTAAAAGGCTTCAAAACTA+TGG | 0.380131 | 1.3:-76142543 | None:intergenic |
| AGTCAGCAACAAACTGTCTC+TGG | 0.381755 | 1.3:+76146149 | MS.gene74960:CDS |
| TGTTATAAAGAAGCCAGAAT+TGG | 0.388043 | 1.3:+76144543 | MS.gene74960:CDS |
| TAAGAATTTACCTTCCCTTC+AGG | 0.388358 | 1.3:-76147377 | None:intergenic |
| TGCTATATCACTATCATTGA+TGG | 0.389779 | 1.3:-76144933 | None:intergenic |
| GAATAGGTACACTGAAATTT+GGG | 0.392508 | 1.3:+76149432 | MS.gene74960:intron |
| GAGCCGTTATTGCTCGTGTT+TGG | 0.395865 | 1.3:-76146290 | None:intergenic |
| TGCAATGACTTCAACCAAAT+CGG | 0.396115 | 1.3:-76141545 | None:intergenic |
| TCGTGTATTCTGTTGTTTGA+CGG | 0.402618 | 1.3:-76144973 | None:intergenic |
| AGCTTTGATGGGGAAACTTA+AGG | 0.404513 | 1.3:+76142898 | MS.gene74960:CDS |
| AGAAGAAGCGAAATCCGATT+TGG | 0.405146 | 1.3:+76141531 | MS.gene74960:CDS |
| ATATTTGATTGTCCTTTCCT+GGG | 0.406458 | 1.3:+76144609 | MS.gene74960:CDS |
| TTGGTTGAAGTCATTGCAAT+TGG | 0.411903 | 1.3:+76141550 | MS.gene74960:CDS |
| CTTCATTAACGGATTCATAA+GGG | 0.412878 | 1.3:-76146474 | None:intergenic |
| GCTCAAAGTTGTCTGCTTCA+AGG | 0.412995 | 1.3:+76146309 | MS.gene74960:CDS |
| GAGATCTTATAACGTTGATA+AGG | 0.414385 | 1.3:+76146202 | MS.gene74960:CDS |
| GGAACATTCTATTTCAAGAA+TGG | 0.415848 | 1.3:+76147583 | MS.gene74960:CDS |
| AGTAGGTTGGGCTCGACATC+AGG | 0.417739 | 1.3:-76145025 | None:intergenic |
| AGATCTTATAACGTTGATAA+GGG | 0.421092 | 1.3:+76146203 | MS.gene74960:CDS |
| CTATATTGTATTTCCACAAC+AGG | 0.424847 | 1.3:-76147317 | None:intergenic |
| TCGGAAGGCCTCACACCAAC+AGG | 0.431900 | 1.3:-76146743 | None:intergenic |
| AAGAATGGAGATATGTTTCA+AGG | 0.440013 | 1.3:+76147598 | MS.gene74960:CDS |
| GTCTTTGAAATTGCCAAAGA+AGG | 0.449174 | 1.3:-76148007 | None:intergenic |
| AACCAGGGGCGTCTTGTTGT+TGG | 0.452032 | 1.3:+76147508 | MS.gene74960:intron |
| CAAAAGAGTGGGCCCAAGAA+AGG | 0.452782 | 1.3:+76146672 | MS.gene74960:CDS |
| TTCTGGAAGGGAAAGGCAAA+TGG | 0.454145 | 1.3:+76147952 | MS.gene74960:CDS |
| TAAACAGTCTTTCGACTCTT+CGG | 0.455530 | 1.3:-76146762 | None:intergenic |
| TGGCACAGCGGCAAGAGATC+AGG | 0.458775 | 1.3:+76147556 | MS.gene74960:CDS |
| CAGTTTCCAAGCTGCTCCCT+AGG | 0.460619 | 1.3:-76146623 | None:intergenic |
| ATAAGGGATAGGACAATTAG+AGG | 0.460878 | 1.3:-76146458 | None:intergenic |
| GGCAATTGTGAGTCCGTTTG+TGG | 0.465780 | 1.3:-76142522 | None:intergenic |
| AAGATACTTACCTAAGTGTA+AGG | 0.466797 | 1.3:-76145060 | None:intergenic |
| GAGCACGACTTCATAAATAG+TGG | 0.468451 | 1.3:-76142063 | None:intergenic |
| AGCATATTCCACCTTCCTGT+TGG | 0.473885 | 1.3:+76146728 | MS.gene74960:CDS |
| GGGCGTCTTGTTGTTGGAGA+TGG | 0.475679 | 1.3:+76147514 | MS.gene74960:CDS |
| TACAGCGGCGGAATTGATAT+CGG | 0.484499 | 1.3:+76141508 | MS.gene74960:CDS |
| GCTTTGACAATTCCTGGGGA+AGG | 0.485402 | 1.3:-76142039 | None:intergenic |
| CAGGAAGGTGGAATATGCTT+AGG | 0.487208 | 1.3:-76146724 | None:intergenic |
| TATGAATCCGTTAATGAAGA+AGG | 0.494377 | 1.3:+76146478 | MS.gene74960:CDS |
| GACAGTCGTGATCAGTAGGT+TGG | 0.494454 | 1.3:-76145038 | None:intergenic |
| CATTGCAATTGGCGGTAGAA+AGG | 0.494720 | 1.3:+76141561 | MS.gene74960:CDS |
| TCTCAGGTAAAAGCTTTGAT+GGG | 0.495980 | 1.3:+76142887 | MS.gene74960:intron |
| TTCTTTGGCAATTTCAAAGA+CGG | 0.496112 | 1.3:+76148009 | MS.gene74960:CDS |
| CAGCTTGGAAACTGGAATCT+GGG | 0.497400 | 1.3:+76146632 | MS.gene74960:CDS |
| GAGCCAAACACGAGCAATAA+CGG | 0.501986 | 1.3:+76146287 | MS.gene74960:CDS |
| ACAAACTGTCTCTGGGAAAG+AGG | 0.503277 | 1.3:+76146157 | MS.gene74960:CDS |
| GGAAACTGGAATCTGGGCCC+TGG | 0.507776 | 1.3:+76146638 | MS.gene74960:CDS |
| TGACAAACACTGCGCAGAGT+TGG | 0.508108 | 1.3:+76146265 | MS.gene74960:CDS |
| GCCAAGGTGGTCTGCCTGAA+GGG | 0.513027 | 1.3:+76147363 | MS.gene74960:CDS |
| GGCAGGTGTGGAAGCAGTGA+TGG | 0.515489 | 1.3:+76146820 | MS.gene74960:CDS |
| ATGCTTAGGTTATCCACCAT+TGG | 0.516196 | 1.3:-76146710 | None:intergenic |
| AGCACGACTTCATAAATAGT+GGG | 0.518051 | 1.3:-76142062 | None:intergenic |
| GTGGGGCTTTGACAATTCCT+GGG | 0.520502 | 1.3:-76142044 | None:intergenic |
| ACAGTCGTGATCAGTAGGTT+GGG | 0.522663 | 1.3:-76145037 | None:intergenic |
| GTTGAAGTCATTGCAATTGG+CGG | 0.525641 | 1.3:+76141553 | MS.gene74960:CDS |
| ATCTCTGCAGAACCTTCCCC+AGG | 0.527558 | 1.3:+76142027 | MS.gene74960:intron |
| GCATGAGCCGGAGGCAGGTG+TGG | 0.529807 | 1.3:+76146808 | MS.gene74960:CDS |
| GGAGATGGAAATATCTATGA+TGG | 0.530169 | 1.3:+76147529 | MS.gene74960:CDS |
| AAGGGAAAGGCAAATGGAGA+GGG | 0.532264 | 1.3:+76147958 | MS.gene74960:CDS |
| ACTGCTTCCACACCTGCCTC+CGG | 0.534605 | 1.3:-76146815 | None:intergenic |
| AAAAGAGTGGGCCCAAGAAA+GGG | 0.535989 | 1.3:+76146673 | MS.gene74960:CDS |
| AGGGAAAGGCAAATGGAGAG+GGG | 0.536953 | 1.3:+76147959 | MS.gene74960:CDS |
| TGGATAGTATACCACCACTC+TGG | 0.537473 | 1.3:-76146079 | None:intergenic |
| TGTTCGTGCAGCATCTATGC+TGG | 0.537748 | 1.3:+76146913 | MS.gene74960:CDS |
| AATTCCGCCGCTGTAACCGT+TGG | 0.540596 | 1.3:-76141500 | None:intergenic |
| CAACAAAGGTCTACTAAACA+AGG | 0.541254 | 1.3:+76141763 | MS.gene74960:CDS |
| TTAACGGATTCATAAGGGAT+AGG | 0.544925 | 1.3:-76146469 | None:intergenic |
| TCCACCTTCCTGTTGGTGTG+AGG | 0.546047 | 1.3:+76146735 | MS.gene74960:CDS |
| GTCAGCAACAAACTGTCTCT+GGG | 0.548258 | 1.3:+76146150 | MS.gene74960:CDS |
| TGCCAAGGTGGTCTGCCTGA+AGG | 0.548874 | 1.3:+76147362 | MS.gene74960:CDS |
| GAAACTGTAAGGTCCACAAA+CGG | 0.552096 | 1.3:+76142509 | MS.gene74960:CDS |
| GGATAGTATACCACCACTCT+GGG | 0.558036 | 1.3:-76146078 | None:intergenic |
| AGGTGGTCTGCCTGAAGGGA+AGG | 0.558342 | 1.3:+76147367 | MS.gene74960:CDS |
| TTCAAAGACGGATGGCGGCA+TGG | 0.564291 | 1.3:+76148021 | MS.gene74960:CDS |
| GCTATATCACTATCATTGAT+GGG | 0.564613 | 1.3:-76144932 | None:intergenic |
| CCGCTGTAACCGTTGGACAA+TGG | 0.565682 | 1.3:-76141493 | None:intergenic |
| TGGTTTGCAAACTTCTGGAA+GGG | 0.569915 | 1.3:+76147940 | MS.gene74960:CDS |
| TTGGCAATTTCAAAGACGGA+TGG | 0.570378 | 1.3:+76148013 | MS.gene74960:CDS |
| TACACTGAAATTTGGGATCA+CGG | 0.572724 | 1.3:+76149439 | MS.gene74960:CDS |
| GCAATTTCAAAGACGGATGG+CGG | 0.577985 | 1.3:+76148016 | MS.gene74960:CDS |
| ACTCTGGGAAGGTTGTTGTA+CGG | 0.578741 | 1.3:-76146063 | None:intergenic |
| TGCAAACTTCTGGAAGGGAA+AGG | 0.579004 | 1.3:+76147945 | MS.gene74960:CDS |
| CAGTCTTTCGACTCTTCGGA+AGG | 0.581994 | 1.3:-76146758 | None:intergenic |
| TAAGGACAGTCGTGATCAGT+AGG | 0.582500 | 1.3:-76145042 | None:intergenic |
| GTGGTTTGCAAACTTCTGGA+AGG | 0.583196 | 1.3:+76147939 | MS.gene74960:CDS |
| AGCTGATGATACACCAATGG+TGG | 0.587823 | 1.3:+76146697 | MS.gene74960:CDS |
| CTCAGGTAAAAGCTTTGATG+GGG | 0.588289 | 1.3:+76142888 | MS.gene74960:intron |
| TCACGACTGTCCTTACACTT+AGG | 0.588645 | 1.3:+76145050 | MS.gene74960:CDS |
| AACAAAGTGGAGTCCTGTTG+TGG | 0.594201 | 1.3:+76147304 | MS.gene74960:CDS |
| TCCCTTCAGGCAGACCACCT+TGG | 0.595487 | 1.3:-76147364 | None:intergenic |
| ATCTTATAACGTTGATAAGG+GGG | 0.598467 | 1.3:+76146205 | MS.gene74960:CDS |
| AGATACAAGGGACGATGCCA+AGG | 0.599966 | 1.3:+76147347 | MS.gene74960:CDS |
| GAGATATGTTTCAAGGATCG+TGG | 0.601230 | 1.3:+76147605 | MS.gene74960:CDS |
| GATCTTATAACGTTGATAAG+GGG | 0.602409 | 1.3:+76146204 | MS.gene74960:CDS |
| AGCGGCAAGAGATCAGGTCC+AGG | 0.603757 | 1.3:+76147562 | MS.gene74960:CDS |
| GATTTGAAGTGGCAAAAGAG+TGG | 0.603772 | 1.3:+76146660 | MS.gene74960:CDS |
| TTCAAAGAAACACCCAGGAA+AGG | 0.604072 | 1.3:-76144621 | None:intergenic |
| GAAGGGAAAGGCAAATGGAG+AGG | 0.613049 | 1.3:+76147957 | MS.gene74960:CDS |
| ATTTGAAGTGGCAAAAGAGT+GGG | 0.615548 | 1.3:+76146661 | MS.gene74960:CDS |
| ATATAGGGGAGGAAGATACA+AGG | 0.618509 | 1.3:+76147334 | MS.gene74960:CDS |
| TCTGGGCCCTGGATTTGAAG+TGG | 0.618907 | 1.3:+76146649 | MS.gene74960:CDS |
| GGATGATGTCATGCACGGCA+AGG | 0.621090 | 1.3:+76147630 | MS.gene74960:CDS |
| AAGAAGCACAATTACAACAA+AGG | 0.624935 | 1.3:+76141749 | MS.gene74960:CDS |
| AACGTGATTTGTCAACAAAG+TGG | 0.625546 | 1.3:+76147291 | MS.gene74960:CDS |
| GTGTGAATGCAAATGGAGAG+AGG | 0.626401 | 1.3:+76148055 | MS.gene74960:CDS |
| GCCTCACACCAACAGGAAGG+TGG | 0.629056 | 1.3:-76146736 | None:intergenic |
| AATCTGTCATCTATAATACT+GGG | 0.629535 | 1.3:-76146242 | None:intergenic |
| AGAAGCCAGAATTGGATTTG+GGG | 0.631126 | 1.3:+76144551 | MS.gene74960:CDS |
| TGGAGGGATGATGTCATGCA+CGG | 0.631513 | 1.3:+76147625 | MS.gene74960:CDS |
| GACAAACTTCCATTGTCCAA+CGG | 0.632152 | 1.3:+76141484 | MS.gene74960:CDS |
| TTGTCCAACGGTTACAGCGG+CGG | 0.635320 | 1.3:+76141496 | MS.gene74960:CDS |
| GTTGTGGAAATACAATATAG+GGG | 0.640990 | 1.3:+76147320 | MS.gene74960:CDS |
| AGTATACCACCACTCTGGGA+AGG | 0.644388 | 1.3:-76146074 | None:intergenic |
| AGACTTGTTGACTCTAGATG+AGG | 0.648376 | 1.3:+76144163 | MS.gene74960:CDS |
| TATGTTTCAAGGATCGTGGA+GGG | 0.652467 | 1.3:+76147609 | MS.gene74960:CDS |
| CCATTGTCCAACGGTTACAG+CGG | 0.654863 | 1.3:+76141493 | MS.gene74960:CDS |
| GGAAGCTGATGATACACCAA+TGG | 0.657220 | 1.3:+76146694 | MS.gene74960:CDS |
| AGTAACAAACCAAAGAAGCA+CGG | 0.658630 | 1.3:+76146881 | MS.gene74960:CDS |
| TCTACACATCACAATGTGCA+CGG | 0.659684 | 1.3:+76141459 | None:intergenic |
| AGGGTTAAGACGAAAAGATG+AGG | 0.662671 | 1.3:+76142571 | MS.gene74960:CDS |
| GAATGTACAAACAAGGACAA+TGG | 0.663850 | 1.3:-76142091 | None:intergenic |
| AAGGCCTCACACCAACAGGA+AGG | 0.668342 | 1.3:-76146739 | None:intergenic |
| TGGGGCTTTGACAATTCCTG+GGG | 0.668612 | 1.3:-76142043 | None:intergenic |
| GTGGAAATACAATATAGGGG+AGG | 0.668898 | 1.3:+76147323 | MS.gene74960:CDS |
| AAATATCAAAAGCGACAGTG+TGG | 0.674008 | 1.3:-76142484 | None:intergenic |
| GTCAAACAACAGAATACACG+AGG | 0.682365 | 1.3:+76144975 | MS.gene74960:CDS |
| GCACGACTTCATAAATAGTG+GGG | 0.684283 | 1.3:-76142061 | None:intergenic |
| CAACAACCTTCCCAGAGTGG+TGG | 0.686943 | 1.3:+76146068 | MS.gene74960:CDS |
| ATATGTTTCAAGGATCGTGG+AGG | 0.691184 | 1.3:+76147608 | MS.gene74960:CDS |
| TACAAGGGACGATGCCAAGG+TGG | 0.695745 | 1.3:+76147350 | MS.gene74960:CDS |
| GAAAGGCAAATGGAGAGGGG+CGG | 0.715092 | 1.3:+76147962 | MS.gene74960:CDS |
| TATAGGGGAGGAAGATACAA+GGG | 0.718321 | 1.3:+76147335 | MS.gene74960:CDS |
| GTACAACAACCTTCCCAGAG+TGG | 0.725672 | 1.3:+76146065 | MS.gene74960:CDS |
| ATACCTTCAAAGAAACACCC+AGG | 0.745843 | 1.3:-76144626 | None:intergenic |
| TATGATGGTTTATGGCACAG+CGG | 0.781523 | 1.3:+76147544 | MS.gene74960:CDS |
| ACACATCACAATGTGCACGG+AGG | 0.784389 | 1.3:+76141462 | None:intergenic |
| TCAAACAACAGAATACACGA+GGG | 0.791232 | 1.3:+76144976 | MS.gene74960:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 76141472 | 76149495 | 76141472 | ID=MS.gene74960 |
| chr1.3 | mRNA | 76141472 | 76149495 | 76141472 | ID=MS.gene74960.t1;Parent=MS.gene74960 |
| chr1.3 | exon | 76141472 | 76141641 | 76141472 | ID=MS.gene74960.t1.exon1;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76141472 | 76141641 | 76141472 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76141726 | 76141784 | 76141726 | ID=MS.gene74960.t1.exon2;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76141726 | 76141784 | 76141726 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76142037 | 76142101 | 76142037 | ID=MS.gene74960.t1.exon3;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76142037 | 76142101 | 76142037 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76142458 | 76142592 | 76142458 | ID=MS.gene74960.t1.exon4;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76142458 | 76142592 | 76142458 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76142893 | 76142941 | 76142893 | ID=MS.gene74960.t1.exon5;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76142893 | 76142941 | 76142893 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76144135 | 76144247 | 76144135 | ID=MS.gene74960.t1.exon6;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76144135 | 76144247 | 76144135 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76144336 | 76144407 | 76144336 | ID=MS.gene74960.t1.exon7;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76144336 | 76144407 | 76144336 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76144534 | 76144644 | 76144534 | ID=MS.gene74960.t1.exon8;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76144534 | 76144644 | 76144534 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76144890 | 76145071 | 76144890 | ID=MS.gene74960.t1.exon9;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76144890 | 76145071 | 76144890 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76146055 | 76146330 | 76146055 | ID=MS.gene74960.t1.exon10;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76146055 | 76146330 | 76146055 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76146426 | 76146499 | 76146426 | ID=MS.gene74960.t1.exon11;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76146426 | 76146499 | 76146426 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76146609 | 76146934 | 76146609 | ID=MS.gene74960.t1.exon12;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76146609 | 76146934 | 76146609 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76147284 | 76147388 | 76147284 | ID=MS.gene74960.t1.exon13;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76147284 | 76147388 | 76147284 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76147514 | 76147651 | 76147514 | ID=MS.gene74960.t1.exon14;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76147514 | 76147651 | 76147514 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76147910 | 76148076 | 76147910 | ID=MS.gene74960.t1.exon15;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76147910 | 76148076 | 76147910 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| chr1.3 | exon | 76149438 | 76149495 | 76149438 | ID=MS.gene74960.t1.exon16;Parent=MS.gene74960.t1 |
| chr1.3 | CDS | 76149438 | 76149495 | 76149438 | ID=cds.MS.gene74960.t1;Parent=MS.gene74960.t1 |
| Gene Sequence |
| Protein sequence |
