Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80355.t1 | KEH25858.1 | 95 | 383 | 16 | 1 | 1 | 383 | 1 | 380 | 1.60E-195 | 692.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80355.t1 | A0A072U9Y9 | 95.0 | 383 | 16 | 1 | 1 | 383 | 1 | 380 | 1.2e-195 | 692.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene80355.t1 | TF | Trihelix |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene80355 | 0.848813 | 4.83E-60 | -1.69E-46 |
| MS.gene049404 | MS.gene80355 | 0.802855 | 4.60E-49 | -1.69E-46 |
| MS.gene049669 | MS.gene80355 | 0.838254 | 3.21E-57 | -1.69E-46 |
| MS.gene050105 | MS.gene80355 | 0.811521 | 6.72E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene80355 | 0.812266 | 4.62E-51 | -1.69E-46 |
| MS.gene050237 | MS.gene80355 | 0.811067 | 8.43E-51 | -1.69E-46 |
| MS.gene050442 | MS.gene80355 | 0.844396 | 7.76E-59 | -1.69E-46 |
| MS.gene050559 | MS.gene80355 | 0.833461 | 5.26E-56 | -1.69E-46 |
| MS.gene050561 | MS.gene80355 | 0.824387 | 8.30E-54 | -1.69E-46 |
| MS.gene050562 | MS.gene80355 | 0.814395 | 1.57E-51 | -1.69E-46 |
| MS.gene050680 | MS.gene80355 | 0.800285 | 1.55E-48 | -1.69E-46 |
| MS.gene050750 | MS.gene80355 | 0.835393 | 1.72E-56 | -1.69E-46 |
| MS.gene050759 | MS.gene80355 | 0.809949 | 1.47E-50 | -1.69E-46 |
| MS.gene050953 | MS.gene80355 | 0.81425 | 1.69E-51 | -1.69E-46 |
| MS.gene050962 | MS.gene80355 | 0.802315 | 5.95E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene80355 | 0.812854 | 3.44E-51 | -1.69E-46 |
| MS.gene051594 | MS.gene80355 | 0.809626 | 1.72E-50 | -1.69E-46 |
| MS.gene051926 | MS.gene80355 | -0.817664 | 2.93E-52 | -1.69E-46 |
| MS.gene052108 | MS.gene80355 | 0.812333 | 4.47E-51 | -1.69E-46 |
| MS.gene052176 | MS.gene80355 | 0.831045 | 2.09E-55 | -1.69E-46 |
| MS.gene052190 | MS.gene80355 | 0.828231 | 1.01E-54 | -1.69E-46 |
| MS.gene052333 | MS.gene80355 | 0.836335 | 9.94E-57 | -1.69E-46 |
| MS.gene052464 | MS.gene80355 | 0.813199 | 2.89E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene80355 | 0.828175 | 1.04E-54 | -1.69E-46 |
| MS.gene052582 | MS.gene80355 | 0.813895 | 2.03E-51 | -1.69E-46 |
| MS.gene052584 | MS.gene80355 | 0.81142 | 7.06E-51 | -1.69E-46 |
| MS.gene052586 | MS.gene80355 | 0.813969 | 1.96E-51 | -1.69E-46 |
| MS.gene052674 | MS.gene80355 | 0.809866 | 1.53E-50 | -1.69E-46 |
| MS.gene052675 | MS.gene80355 | 0.812049 | 5.16E-51 | -1.69E-46 |
| MS.gene052677 | MS.gene80355 | 0.814648 | 1.38E-51 | -1.69E-46 |
| MS.gene052954 | MS.gene80355 | 0.810724 | 1.00E-50 | -1.69E-46 |
| MS.gene053006 | MS.gene80355 | 0.829943 | 3.88E-55 | -1.69E-46 |
| MS.gene053085 | MS.gene80355 | 0.807611 | 4.65E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene80355 | 0.81601 | 6.89E-52 | -1.69E-46 |
| MS.gene053201 | MS.gene80355 | 0.835083 | 2.06E-56 | -1.69E-46 |
| MS.gene05414 | MS.gene80355 | 0.818421 | 1.98E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene80355 | 0.842539 | 2.43E-58 | -1.69E-46 |
| MS.gene054291 | MS.gene80355 | -0.836132 | 1.12E-56 | -1.69E-46 |
| MS.gene054405 | MS.gene80355 | 0.803035 | 4.22E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene80355 | 0.820389 | 7.04E-53 | -1.69E-46 |
| MS.gene054479 | MS.gene80355 | 0.802624 | 5.14E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene80355 | 0.824016 | 1.01E-53 | -1.69E-46 |
| MS.gene054657 | MS.gene80355 | 0.808857 | 2.52E-50 | -1.69E-46 |
| MS.gene05510 | MS.gene80355 | 0.803028 | 4.24E-49 | -1.69E-46 |
| MS.gene055228 | MS.gene80355 | 0.816402 | 5.63E-52 | -1.69E-46 |
| MS.gene055230 | MS.gene80355 | 0.809112 | 2.22E-50 | -1.69E-46 |
| MS.gene055701 | MS.gene80355 | 0.836012 | 1.20E-56 | -1.69E-46 |
| MS.gene055818 | MS.gene80355 | 0.841242 | 5.35E-58 | -1.69E-46 |
| MS.gene055841 | MS.gene80355 | 0.821787 | 3.35E-53 | -1.69E-46 |
| MS.gene056162 | MS.gene80355 | 0.822165 | 2.74E-53 | -1.69E-46 |
| MS.gene056174 | MS.gene80355 | 0.801971 | 7.00E-49 | -1.69E-46 |
| MS.gene056498 | MS.gene80355 | 0.80509 | 1.58E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene80355 | 0.850202 | 1.98E-60 | -1.69E-46 |
| MS.gene05659 | MS.gene80355 | 0.80357 | 3.27E-49 | -1.69E-46 |
| MS.gene056776 | MS.gene80355 | 0.815757 | 7.85E-52 | -1.69E-46 |
| MS.gene056779 | MS.gene80355 | 0.840063 | 1.09E-57 | -1.69E-46 |
| MS.gene056873 | MS.gene80355 | 0.807721 | 4.40E-50 | -1.69E-46 |
| MS.gene057026 | MS.gene80355 | 0.826396 | 2.78E-54 | -1.69E-46 |
| MS.gene057060 | MS.gene80355 | 0.815197 | 1.05E-51 | -1.69E-46 |
| MS.gene057156 | MS.gene80355 | -0.833149 | 6.30E-56 | -1.69E-46 |
| MS.gene057208 | MS.gene80355 | 0.820564 | 6.42E-53 | -1.69E-46 |
| MS.gene05761 | MS.gene80355 | 0.848846 | 4.73E-60 | -1.69E-46 |
| MS.gene057693 | MS.gene80355 | 0.80871 | 2.71E-50 | -1.69E-46 |
| MS.gene057869 | MS.gene80355 | 0.808925 | 2.44E-50 | -1.69E-46 |
| MS.gene058009 | MS.gene80355 | 0.803625 | 3.19E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene80355 | 0.811722 | 6.07E-51 | -1.69E-46 |
| MS.gene058625 | MS.gene80355 | 0.821421 | 4.08E-53 | -1.69E-46 |
| MS.gene058979 | MS.gene80355 | 0.806576 | 7.69E-50 | -1.69E-46 |
| MS.gene059317 | MS.gene80355 | 0.805318 | 1.41E-49 | -1.69E-46 |
| MS.gene059584 | MS.gene80355 | 0.813295 | 2.75E-51 | -1.69E-46 |
| MS.gene060011 | MS.gene80355 | 0.824242 | 8.98E-54 | -1.69E-46 |
| MS.gene060167 | MS.gene80355 | 0.809083 | 2.26E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene80355 | 0.856928 | 2.31E-62 | -1.69E-46 |
| MS.gene060521 | MS.gene80355 | 0.806625 | 7.51E-50 | -1.69E-46 |
| MS.gene06052 | MS.gene80355 | 0.817824 | 2.70E-52 | -1.69E-46 |
| MS.gene060553 | MS.gene80355 | 0.801458 | 8.92E-49 | -1.69E-46 |
| MS.gene060683 | MS.gene80355 | 0.827926 | 1.19E-54 | -1.69E-46 |
| MS.gene060685 | MS.gene80355 | 0.832095 | 1.15E-55 | -1.69E-46 |
| MS.gene060840 | MS.gene80355 | 0.805136 | 1.54E-49 | -1.69E-46 |
| MS.gene060879 | MS.gene80355 | 0.808361 | 3.22E-50 | -1.69E-46 |
| MS.gene060918 | MS.gene80355 | 0.811208 | 7.85E-51 | -1.69E-46 |
| MS.gene061040 | MS.gene80355 | -0.82751 | 1.50E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80355.t1 | MTR_6g035315 | 95.082 | 427 | 21 | 0 | 25 | 451 | 22 | 448 | 0.0 | 741 |
| MS.gene80355.t1 | MTR_1g098920 | 70.396 | 429 | 114 | 6 | 24 | 445 | 23 | 445 | 0.0 | 583 |
| MS.gene80355.t1 | MTR_2g098080 | 41.687 | 415 | 210 | 6 | 37 | 444 | 22 | 411 | 2.62e-90 | 281 |
| MS.gene80355.t1 | MTR_7g103390 | 49.590 | 244 | 102 | 6 | 11 | 248 | 1 | 229 | 1.82e-65 | 217 |
| MS.gene80355.t1 | MTR_1g061640 | 61.783 | 157 | 57 | 3 | 93 | 248 | 95 | 249 | 2.37e-57 | 197 |
| MS.gene80355.t1 | MTR_1g061640 | 49.333 | 75 | 38 | 0 | 371 | 445 | 406 | 480 | 5.18e-18 | 86.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80355.t1 | AT1G21200 | 61.488 | 457 | 148 | 10 | 1 | 446 | 1 | 440 | 1.54e-178 | 506 |
| MS.gene80355.t1 | AT1G21200 | 61.488 | 457 | 148 | 10 | 1 | 446 | 1 | 440 | 1.54e-178 | 506 |
| MS.gene80355.t1 | AT1G76870 | 52.283 | 438 | 145 | 15 | 17 | 447 | 3 | 383 | 2.50e-126 | 372 |
| MS.gene80355.t1 | AT1G76870 | 52.283 | 438 | 145 | 15 | 17 | 447 | 3 | 383 | 2.50e-126 | 372 |
| MS.gene80355.t1 | AT1G76870 | 52.283 | 438 | 145 | 15 | 17 | 447 | 3 | 383 | 2.50e-126 | 372 |
| MS.gene80355.t1 | AT3G10040 | 44.223 | 251 | 110 | 8 | 21 | 252 | 7 | 246 | 3.79e-45 | 163 |
Find 114 sgRNAs with CRISPR-Local
Find 138 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTGCAGTGTTGCAGAAAA+AGG | 0.201562 | 6.3:+27587569 | MS.gene80355:CDS |
| CATGAAGATGCTACTACTTT+TGG | 0.214221 | 6.3:+27588104 | MS.gene80355:CDS |
| AGAAGGAAGTAGAGGGAAAA+AGG | 0.214962 | 6.3:+27587431 | MS.gene80355:CDS |
| AATGCAATCTATTACAATTA+TGG | 0.268261 | 6.3:-27587848 | None:intergenic |
| AATTCAACGATCTTAATAAA+AGG | 0.306147 | 6.3:+27587664 | MS.gene80355:CDS |
| TATCATGATCATCTCTATTT+CGG | 0.312915 | 6.3:-27587914 | None:intergenic |
| ATCTTAATAAAAGGTATAAA+AGG | 0.318059 | 6.3:+27587673 | MS.gene80355:CDS |
| TGGGAATTCTTTAAATTGTC+AGG | 0.360558 | 6.3:+27588124 | MS.gene80355:CDS |
| GGTGATAGTCGAGGAACCTT+TGG | 0.361077 | 6.3:+27588038 | MS.gene80355:CDS |
| GTGATAGTCGAGGAACCTTT+GGG | 0.376431 | 6.3:+27588039 | MS.gene80355:CDS |
| TGACGAGGATGATCATGATA+TGG | 0.392416 | 6.3:+27587971 | MS.gene80355:CDS |
| CAAAAGTAGTAGCATCTTCA+TGG | 0.393797 | 6.3:-27588103 | None:intergenic |
| CTGTCAAGTTGTTGAAAATC+CGG | 0.402795 | 6.3:+27587725 | MS.gene80355:CDS |
| CGGCGGCACCAGCTTTAACT+AGG | 0.419109 | 6.3:+27588445 | MS.gene80355:CDS |
| GTCGATGAACGAGTTTAGTC+AGG | 0.421399 | 6.3:+27587341 | MS.gene80355:CDS |
| TTCAACGTCCCTATTGTAAG+AGG | 0.429215 | 6.3:-27587300 | None:intergenic |
| TAAGTGGAAATCTATATCTA+AGG | 0.434785 | 6.3:+27587593 | MS.gene80355:CDS |
| CAAGGTGATGGTGGTGGTGG+TGG | 0.436461 | 6.3:+27587144 | MS.gene80355:CDS |
| GAAATCTATATCTAAGGTTA+TGG | 0.439671 | 6.3:+27587599 | MS.gene80355:CDS |
| TGTAAGGACCGTTGCAATGC+AGG | 0.440711 | 6.3:-27587882 | None:intergenic |
| AGATGGAAATCAAGCTTTAC+CGG | 0.441260 | 6.3:+27588190 | MS.gene80355:CDS |
| GTTCAAGGTGATGGTGGTGG+TGG | 0.446247 | 6.3:+27587141 | MS.gene80355:CDS |
| ATTTCGGAGAGCTATTTGTA+AGG | 0.472772 | 6.3:-27587898 | None:intergenic |
| GTGCTTTCTTCGATTAATGA+AGG | 0.476946 | 6.3:+27587273 | MS.gene80355:CDS |
| TAGTAGCATCTTCATGGCCT+TGG | 0.479201 | 6.3:-27588097 | None:intergenic |
| GGTAAAGCTTGATTTCCATC+TGG | 0.481200 | 6.3:-27588188 | None:intergenic |
| AACTCATCCGTGTCAAGGGC+CGG | 0.484357 | 6.3:+27587248 | MS.gene80355:CDS |
| ACAGATTCAGATTGAGATGA+TGG | 0.485981 | 6.3:+27588286 | MS.gene80355:CDS |
| TGTTGAAAATCCGGCTTTGT+TGG | 0.486943 | 6.3:+27587734 | MS.gene80355:CDS |
| TCTCAAGGAGGTATAGTTCA+AGG | 0.489422 | 6.3:+27587126 | MS.gene80355:CDS |
| CGCCTTGGCAGCGTGTGAAG+TGG | 0.492977 | 6.3:+27587457 | MS.gene80355:CDS |
| GGGAGTTCTGAAGGTGGTAG+TGG | 0.493151 | 6.3:+27587534 | MS.gene80355:CDS |
| AAGCTTGATTTCCATCTGGT+TGG | 0.498671 | 6.3:-27588184 | None:intergenic |
| CAGCTTGGTTACAGAAGCAA+TGG | 0.506444 | 6.3:+27588225 | MS.gene80355:CDS |
| AAAAGGCTTAACGATATGCT+TGG | 0.506945 | 6.3:+27587690 | MS.gene80355:CDS |
| CGATGAACTCGCATCGATCC+CGG | 0.508975 | 6.3:-27587189 | None:intergenic |
| CTGAAGGTGGTAGTGGTGGA+AGG | 0.527560 | 6.3:+27587541 | MS.gene80355:CDS |
| CTCGTCATGATCATCATGAT+AGG | 0.537593 | 6.3:-27587955 | None:intergenic |
| GAGGAAGATGAGCCGGAGGA+AGG | 0.539815 | 6.3:+27587390 | MS.gene80355:CDS |
| TCGATTACATCCAACAAAGC+CGG | 0.539815 | 6.3:-27587744 | None:intergenic |
| GTGTCTTATATTGCCGAGGA+CGG | 0.542481 | 6.3:+27587513 | MS.gene80355:CDS |
| AGCTTGGTTACAGAAGCAAT+GGG | 0.542768 | 6.3:+27588226 | MS.gene80355:CDS |
| GAGCCGGAGGAAGGTGGTGA+TGG | 0.542968 | 6.3:+27587399 | MS.gene80355:CDS |
| GGCTTAACGATATGCTTGGA+AGG | 0.546544 | 6.3:+27587694 | MS.gene80355:CDS |
| CATAAAAGTTCATATCCTCA+TGG | 0.548000 | 6.3:+27588152 | MS.gene80355:CDS |
| ATATCTTAACGAGAAAGAAA+AGG | 0.550124 | 6.3:+27587767 | MS.gene80355:CDS |
| GAACTGAAGCGAAAGGAGAT+CGG | 0.553087 | 6.3:+27588425 | MS.gene80355:CDS |
| CATCAAACTCATCCGTGTCA+AGG | 0.557158 | 6.3:+27587243 | MS.gene80355:CDS |
| TGTCTTATATTGCCGAGGAC+GGG | 0.557593 | 6.3:+27587514 | MS.gene80355:CDS |
| GGGGAGTGAGGAAGATGAGC+CGG | 0.557735 | 6.3:+27587383 | MS.gene80355:CDS |
| CGGAAAACATGAAAGCAGCT+TGG | 0.558783 | 6.3:+27588210 | MS.gene80355:CDS |
| ATCAAACTCATCCGTGTCAA+GGG | 0.560403 | 6.3:+27587244 | MS.gene80355:CDS |
| GACACGGATGAGTTTGATGT+TGG | 0.561258 | 6.3:-27587239 | None:intergenic |
| GCCGAGGACGGGAGTTCTGA+AGG | 0.563055 | 6.3:+27587525 | MS.gene80355:CDS |
| ATAGTTCAAGGTGATGGTGG+TGG | 0.564193 | 6.3:+27587138 | MS.gene80355:CDS |
| AGAGTTGGAGAAGTTCAAGC+TGG | 0.565535 | 6.3:+27588361 | MS.gene80355:CDS |
| TGATGTTGGTTCATCGTATG+CGG | 0.567222 | 6.3:-27587225 | None:intergenic |
| GGATGATCATGATATGGAAA+CGG | 0.567609 | 6.3:+27587977 | MS.gene80355:CDS |
| GGACAGACGGTCCAACCAGA+TGG | 0.572770 | 6.3:+27588173 | MS.gene80355:CDS |
| TCTAAGGTTATGGCTGAAAG+AGG | 0.573389 | 6.3:+27587609 | MS.gene80355:CDS |
| CGCACGATCCTGCATTGCAA+CGG | 0.573999 | 6.3:+27587874 | MS.gene80355:CDS |
| GCTTGAACTTCTCCAACTCT+CGG | 0.574294 | 6.3:-27588358 | None:intergenic |
| GGTATAGTTCAAGGTGATGG+TGG | 0.576507 | 6.3:+27587135 | MS.gene80355:CDS |
| AGCACCGCCGGCCCTTGACA+CGG | 0.577678 | 6.3:-27587255 | None:intergenic |
| GCTTAACGATATGCTTGGAA+GGG | 0.580590 | 6.3:+27587695 | MS.gene80355:CDS |
| GGAGGTATAGTTCAAGGTGA+TGG | 0.582990 | 6.3:+27587132 | MS.gene80355:CDS |
| TGCTTTAGAACTGAAGCGAA+AGG | 0.583633 | 6.3:+27588418 | MS.gene80355:CDS |
| ACCTTCAGAACTCCCGTCCT+CGG | 0.585198 | 6.3:-27587526 | None:intergenic |
| AAACAAACACTCGGGGAGTG+AGG | 0.585505 | 6.3:+27587371 | MS.gene80355:CDS |
| GTCCACTTCACACGCTGCCA+AGG | 0.587195 | 6.3:-27587459 | None:intergenic |
| TTAGCAAACCTAGTTAAAGC+TGG | 0.588173 | 6.3:-27588453 | None:intergenic |
| TGACCATCACCACCTTCCTC+CGG | 0.589498 | 6.3:-27587402 | None:intergenic |
| CGTTGCAATGCAGGATCGTG+CGG | 0.590521 | 6.3:-27587873 | None:intergenic |
| GGAGATAGAAACAAACACTC+GGG | 0.601048 | 6.3:+27587363 | MS.gene80355:CDS |
| CTTAACGATATGCTTGGAAG+GGG | 0.602105 | 6.3:+27587696 | MS.gene80355:CDS |
| GATAGTCGAGGAACCTTTGG+GGG | 0.602548 | 6.3:+27588041 | MS.gene80355:CDS |
| GAGTGAGGAAGATGAGCCGG+AGG | 0.604600 | 6.3:+27587386 | MS.gene80355:CDS |
| CATCATCAAGAAGGAAGTAG+AGG | 0.606503 | 6.3:+27587423 | MS.gene80355:CDS |
| AGTTCTGAAGGTGGTAGTGG+TGG | 0.609983 | 6.3:+27587537 | MS.gene80355:CDS |
| ATGATAACGACGATGTGAGA+AGG | 0.612473 | 6.3:+27587931 | MS.gene80355:CDS |
| TTCATATCCTCATGGACAGA+CGG | 0.612854 | 6.3:+27588160 | MS.gene80355:CDS |
| TCGATGCGAGTTCATCGTCA+AGG | 0.616549 | 6.3:+27587195 | MS.gene80355:CDS |
| AATTTATCCTCACATTGCTG+AGG | 0.616974 | 6.3:-27587645 | None:intergenic |
| GAAGATGAGCCGGAGGAAGG+TGG | 0.617091 | 6.3:+27587393 | MS.gene80355:CDS |
| GGTGATGGTCATCATCAAGA+AGG | 0.617927 | 6.3:+27587414 | MS.gene80355:CDS |
| GAAGAGACCTACGCTTCCCA+TGG | 0.620396 | 6.3:+27588017 | MS.gene80355:CDS |
| TATGGAAACGGACGATCATG+AGG | 0.621056 | 6.3:+27587989 | MS.gene80355:CDS |
| ACTATCACCATGGGAAGCGT+AGG | 0.622507 | 6.3:-27588024 | None:intergenic |
| TGTGAAGTGGACTGATAAGA+TGG | 0.627029 | 6.3:+27587470 | MS.gene80355:CDS |
| ATCATCAAGAAGGAAGTAGA+GGG | 0.627850 | 6.3:+27587424 | MS.gene80355:CDS |
| GGGAGATAGAAACAAACACT+CGG | 0.632973 | 6.3:+27587362 | MS.gene80355:CDS |
| CGATGCGAGTTCATCGTCAA+GGG | 0.633456 | 6.3:+27587196 | MS.gene80355:CDS |
| TTAATCGAAGAAAGCACCGC+CGG | 0.638253 | 6.3:-27587267 | None:intergenic |
| ATGGAAGGGAATTTGTCTCA+AGG | 0.640788 | 6.3:+27587111 | MS.gene80355:CDS |
| TGTTGTTGAGAAGAAATGGA+AGG | 0.642321 | 6.3:+27587096 | None:intergenic |
| GTTGTTGAGAAGAAATGGAA+GGG | 0.643736 | 6.3:+27587097 | None:intergenic |
| GCAATGGGTTGAATCTCGCT+CGG | 0.646017 | 6.3:+27588241 | MS.gene80355:CDS |
| TCGATGAACGAGTTTAGTCA+GGG | 0.648981 | 6.3:+27587342 | MS.gene80355:CDS |
| TCATCCGTGTCAAGGGCCGG+CGG | 0.649182 | 6.3:+27587251 | MS.gene80355:CDS |
| GCTTCCCATGGTGATAGTCG+AGG | 0.651659 | 6.3:+27588029 | MS.gene80355:CDS |
| TGATAGTCGAGGAACCTTTG+GGG | 0.656509 | 6.3:+27588040 | MS.gene80355:CDS |
| GGTTCCTCGACTATCACCAT+GGG | 0.658511 | 6.3:-27588033 | None:intergenic |
| AGGTTCCTCGACTATCACCA+TGG | 0.658616 | 6.3:-27588034 | None:intergenic |
| GGCGGTGTCTTATATTGCCG+AGG | 0.660613 | 6.3:+27587509 | MS.gene80355:CDS |
| CTATCATGATGATCATGACG+AGG | 0.661777 | 6.3:+27587956 | MS.gene80355:CDS |
| GGTTGGACCGTCTGTCCATG+AGG | 0.661820 | 6.3:-27588167 | None:intergenic |
| AAAAGACTAAGACAAGGCCA+AGG | 0.689910 | 6.3:+27588080 | MS.gene80355:CDS |
| CTGAAGCGAAAGGAGATCGG+CGG | 0.699525 | 6.3:+27588428 | MS.gene80355:CDS |
| TGTTTCGCCTCAGCAATGTG+AGG | 0.699798 | 6.3:+27587638 | MS.gene80355:CDS |
| GAAGGGAATTTGTCTCAAGG+AGG | 0.706788 | 6.3:+27587114 | MS.gene80355:CDS |
| GAGATAGAAACAAACACTCG+GGG | 0.708162 | 6.3:+27587364 | MS.gene80355:CDS |
| GAGGACGGGAGTTCTGAAGG+TGG | 0.721621 | 6.3:+27587528 | MS.gene80355:CDS |
| AGTGGACTGATAAGATGGTG+AGG | 0.726752 | 6.3:+27587475 | MS.gene80355:CDS |
| ACTACGAAAAGACTAAGACA+AGG | 0.735602 | 6.3:+27588074 | MS.gene80355:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCTTAATAAAAGGTATAAA+AGG | + | chr6.3:27587673-27587692 | MS.gene80355:CDS | 15.0% |
| !! | AATGCAATCTATTACAATTA+TGG | - | chr6.3:27587851-27587870 | None:intergenic | 20.0% |
| !! | AATTCAACGATCTTAATAAA+AGG | + | chr6.3:27587664-27587683 | MS.gene80355:CDS | 20.0% |
| !! | AGAGAAACAAAAATTCAAAT+GGG | + | chr6.3:27588313-27588332 | MS.gene80355:CDS | 20.0% |
| !! | TAGAGAAACAAAAATTCAAA+TGG | + | chr6.3:27588312-27588331 | MS.gene80355:CDS | 20.0% |
| ! | ATATCTTAACGAGAAAGAAA+AGG | + | chr6.3:27587767-27587786 | MS.gene80355:CDS | 25.0% |
| ! | TAAGTGGAAATCTATATCTA+AGG | + | chr6.3:27587593-27587612 | MS.gene80355:CDS | 25.0% |
| ! | TATCATGATCATCTCTATTT+CGG | - | chr6.3:27587917-27587936 | None:intergenic | 25.0% |
| !! | GAAATCTATATCTAAGGTTA+TGG | + | chr6.3:27587599-27587618 | MS.gene80355:CDS | 25.0% |
| AACAAAAATTCAAATGGGAG+AGG | + | chr6.3:27588318-27588337 | MS.gene80355:CDS | 30.0% | |
| CATAAAAGTTCATATCCTCA+TGG | + | chr6.3:27588152-27588171 | MS.gene80355:CDS | 30.0% | |
| TGGGAATTCTTTAAATTGTC+AGG | + | chr6.3:27588124-27588143 | MS.gene80355:CDS | 30.0% | |
| ! | AAGGTTTTCCTCTTACAATA+GGG | + | chr6.3:27587292-27587311 | MS.gene80355:CDS | 30.0% |
| ! | CATGAGGATATGAACTTTTA+TGG | - | chr6.3:27588154-27588173 | None:intergenic | 30.0% |
| !! | ATGAAGATGCTACTACTTTT+GGG | + | chr6.3:27588105-27588124 | MS.gene80355:CDS | 30.0% |
| AAAAGGCTTAACGATATGCT+TGG | + | chr6.3:27587690-27587709 | MS.gene80355:CDS | 35.0% | |
| AATTTATCCTCACATTGCTG+AGG | - | chr6.3:27587648-27587667 | None:intergenic | 35.0% | |
| ACAGATTCAGATTGAGATGA+TGG | + | chr6.3:27588286-27588305 | MS.gene80355:CDS | 35.0% | |
| ACTACGAAAAGACTAAGACA+AGG | + | chr6.3:27588074-27588093 | MS.gene80355:CDS | 35.0% | |
| AGATGGAAATCAAGCTTTAC+CGG | + | chr6.3:27588190-27588209 | MS.gene80355:CDS | 35.0% | |
| ATCATCAAGAAGGAAGTAGA+GGG | + | chr6.3:27587424-27587443 | MS.gene80355:CDS | 35.0% | |
| ATTTCGGAGAGCTATTTGTA+AGG | - | chr6.3:27587901-27587920 | None:intergenic | 35.0% | |
| ATTTGCAGTGTTGCAGAAAA+AGG | + | chr6.3:27587569-27587588 | MS.gene80355:CDS | 35.0% | |
| CAAAAGTAGTAGCATCTTCA+TGG | - | chr6.3:27588106-27588125 | None:intergenic | 35.0% | |
| GGATGATCATGATATGGAAA+CGG | + | chr6.3:27587977-27587996 | MS.gene80355:CDS | 35.0% | |
| GTGCTTTCTTCGATTAATGA+AGG | + | chr6.3:27587273-27587292 | MS.gene80355:CDS | 35.0% | |
| TTTGCAGTGTTGCAGAAAAA+GGG | + | chr6.3:27587570-27587589 | MS.gene80355:CDS | 35.0% | |
| ! | ATTTTCAACAACTTGACAGG+AGG | - | chr6.3:27587724-27587743 | None:intergenic | 35.0% |
| ! | CATGAAGATGCTACTACTTT+TGG | + | chr6.3:27588104-27588123 | MS.gene80355:CDS | 35.0% |
| ! | CTGTCAAGTTGTTGAAAATC+CGG | + | chr6.3:27587725-27587744 | MS.gene80355:CDS | 35.0% |
| ! | GAAGGTTTTCCTCTTACAAT+AGG | + | chr6.3:27587291-27587310 | MS.gene80355:CDS | 35.0% |
| !!! | TTTTGGTGGTTTTGATTTGC+CGG | + | chr6.3:27587170-27587189 | MS.gene80355:CDS | 35.0% |
| AAAAGACTAAGACAAGGCCA+AGG | + | chr6.3:27588080-27588099 | MS.gene80355:CDS | 40.0% | |
| AAGCTTGATTTCCATCTGGT+TGG | - | chr6.3:27588187-27588206 | None:intergenic | 40.0% | |
| AAGGAAGTAGAGGGAAAAAG+GGG | + | chr6.3:27587433-27587452 | MS.gene80355:CDS | 40.0% | |
| AGAAGGAAGTAGAGGGAAAA+AGG | + | chr6.3:27587431-27587450 | MS.gene80355:CDS | 40.0% | |
| AGCTTGGTTACAGAAGCAAT+GGG | + | chr6.3:27588226-27588245 | MS.gene80355:CDS | 40.0% | |
| ATCAAACTCATCCGTGTCAA+GGG | + | chr6.3:27587244-27587263 | MS.gene80355:CDS | 40.0% | |
| ATGGAAGGGAATTTGTCTCA+AGG | + | chr6.3:27587111-27587130 | MS.gene80355:CDS | 40.0% | |
| CAAGAAAAAAGACCGAGAGT+TGG | + | chr6.3:27588346-27588365 | MS.gene80355:CDS | 40.0% | |
| CATCATCAAGAAGGAAGTAG+AGG | + | chr6.3:27587423-27587442 | MS.gene80355:CDS | 40.0% | |
| CTATCATGATGATCATGACG+AGG | + | chr6.3:27587956-27587975 | MS.gene80355:CDS | 40.0% | |
| CTCGTCATGATCATCATGAT+AGG | - | chr6.3:27587958-27587977 | None:intergenic | 40.0% | |
| CTTAACGATATGCTTGGAAG+GGG | + | chr6.3:27587696-27587715 | MS.gene80355:CDS | 40.0% | |
| GAAGGAAGTAGAGGGAAAAA+GGG | + | chr6.3:27587432-27587451 | MS.gene80355:CDS | 40.0% | |
| GAGATAGAAACAAACACTCG+GGG | + | chr6.3:27587364-27587383 | MS.gene80355:CDS | 40.0% | |
| GCTTAACGATATGCTTGGAA+GGG | + | chr6.3:27587695-27587714 | MS.gene80355:CDS | 40.0% | |
| GGAGATAGAAACAAACACTC+GGG | + | chr6.3:27587363-27587382 | MS.gene80355:CDS | 40.0% | |
| GGGAGATAGAAACAAACACT+CGG | + | chr6.3:27587362-27587381 | MS.gene80355:CDS | 40.0% | |
| GGTAAAGCTTGATTTCCATC+TGG | - | chr6.3:27588191-27588210 | None:intergenic | 40.0% | |
| TCGATGAACGAGTTTAGTCA+GGG | + | chr6.3:27587342-27587361 | MS.gene80355:CDS | 40.0% | |
| TCGATTACATCCAACAAAGC+CGG | - | chr6.3:27587747-27587766 | None:intergenic | 40.0% | |
| TGACGAGGATGATCATGATA+TGG | + | chr6.3:27587971-27587990 | MS.gene80355:CDS | 40.0% | |
| TGTTGCAGAAAAAGGGTAAG+TGG | + | chr6.3:27587577-27587596 | MS.gene80355:CDS | 40.0% | |
| TTCAACGTCCCTATTGTAAG+AGG | - | chr6.3:27587303-27587322 | None:intergenic | 40.0% | |
| TTCATATCCTCATGGACAGA+CGG | + | chr6.3:27588160-27588179 | MS.gene80355:CDS | 40.0% | |
| ! | ATGATAACGACGATGTGAGA+AGG | + | chr6.3:27587931-27587950 | MS.gene80355:CDS | 40.0% |
| ! | CGGATTTTCAACAACTTGAC+AGG | - | chr6.3:27587727-27587746 | None:intergenic | 40.0% |
| ! | GATGGTGAGGCTTTTGATAA+CGG | + | chr6.3:27587488-27587507 | MS.gene80355:CDS | 40.0% |
| ! | TCTAAGGTTATGGCTGAAAG+AGG | + | chr6.3:27587609-27587628 | MS.gene80355:CDS | 40.0% |
| ! | TCTCAAGGAGGTATAGTTCA+AGG | + | chr6.3:27587126-27587145 | MS.gene80355:CDS | 40.0% |
| ! | TGTTGAAAATCCGGCTTTGT+TGG | + | chr6.3:27587734-27587753 | MS.gene80355:CDS | 40.0% |
| !! | CAAGCTGCTTTCATGTTTTC+CGG | - | chr6.3:27588212-27588231 | None:intergenic | 40.0% |
| !! | TGATGTTGGTTCATCGTATG+CGG | - | chr6.3:27587228-27587247 | None:intergenic | 40.0% |
| !! | TGCTTTAGAACTGAAGCGAA+AGG | + | chr6.3:27588418-27588437 | MS.gene80355:CDS | 40.0% |
| !! | TGTGAAGTGGACTGATAAGA+TGG | + | chr6.3:27587470-27587489 | MS.gene80355:CDS | 40.0% |
| !!! | TTTGGTGGTTTTGATTTGCC+GGG | + | chr6.3:27587171-27587190 | MS.gene80355:CDS | 40.0% |
| AGAGTTGGAGAAGTTCAAGC+TGG | + | chr6.3:27588361-27588380 | MS.gene80355:CDS | 45.0% | |
| CAGCTTGGTTACAGAAGCAA+TGG | + | chr6.3:27588225-27588244 | MS.gene80355:CDS | 45.0% | |
| CATCAAACTCATCCGTGTCA+AGG | + | chr6.3:27587243-27587262 | MS.gene80355:CDS | 45.0% | |
| CGGAAAACATGAAAGCAGCT+TGG | + | chr6.3:27588210-27588229 | MS.gene80355:CDS | 45.0% | |
| GAACTGAAGCGAAAGGAGAT+CGG | + | chr6.3:27588425-27588444 | MS.gene80355:CDS | 45.0% | |
| GAAGGGAATTTGTCTCAAGG+AGG | + | chr6.3:27587114-27587133 | MS.gene80355:CDS | 45.0% | |
| GACACGGATGAGTTTGATGT+TGG | - | chr6.3:27587242-27587261 | None:intergenic | 45.0% | |
| GCTTGAACTTCTCCAACTCT+CGG | - | chr6.3:27588361-27588380 | None:intergenic | 45.0% | |
| GGCTTAACGATATGCTTGGA+AGG | + | chr6.3:27587694-27587713 | MS.gene80355:CDS | 45.0% | |
| GTCGATGAACGAGTTTAGTC+AGG | + | chr6.3:27587341-27587360 | MS.gene80355:CDS | 45.0% | |
| GTGATAGTCGAGGAACCTTT+GGG | + | chr6.3:27588039-27588058 | MS.gene80355:CDS | 45.0% | |
| GTGTCTTATATTGCCGAGGA+CGG | + | chr6.3:27587513-27587532 | MS.gene80355:CDS | 45.0% | |
| TAGTAGCATCTTCATGGCCT+TGG | - | chr6.3:27588100-27588119 | None:intergenic | 45.0% | |
| TATGGAAACGGACGATCATG+AGG | + | chr6.3:27587989-27588008 | MS.gene80355:CDS | 45.0% | |
| TGATAGTCGAGGAACCTTTG+GGG | + | chr6.3:27588040-27588059 | MS.gene80355:CDS | 45.0% | |
| TGTCTTATATTGCCGAGGAC+GGG | + | chr6.3:27587514-27587533 | MS.gene80355:CDS | 45.0% | |
| ! | GGAGGTATAGTTCAAGGTGA+TGG | + | chr6.3:27587132-27587151 | MS.gene80355:CDS | 45.0% |
| ! | GGTATAGTTCAAGGTGATGG+TGG | + | chr6.3:27587135-27587154 | MS.gene80355:CDS | 45.0% |
| ! | GGTGATGGTCATCATCAAGA+AGG | + | chr6.3:27587414-27587433 | MS.gene80355:CDS | 45.0% |
| !! | AGTGGACTGATAAGATGGTG+AGG | + | chr6.3:27587475-27587494 | MS.gene80355:CDS | 45.0% |
| !! | ATAGTTCAAGGTGATGGTGG+TGG | + | chr6.3:27587138-27587157 | MS.gene80355:CDS | 45.0% |
| !! | TTAATCGAAGAAAGCACCGC+CGG | - | chr6.3:27587270-27587289 | None:intergenic | 45.0% |
| AAACAAACACTCGGGGAGTG+AGG | + | chr6.3:27587371-27587390 | MS.gene80355:CDS | 50.0% | |
| AGGTTCCTCGACTATCACCA+TGG | - | chr6.3:27588037-27588056 | None:intergenic | 50.0% | |
| CGATGCGAGTTCATCGTCAA+GGG | + | chr6.3:27587196-27587215 | MS.gene80355:CDS | 50.0% | |
| GATAGTCGAGGAACCTTTGG+GGG | + | chr6.3:27588041-27588060 | MS.gene80355:CDS | 50.0% | |
| GGTGATAGTCGAGGAACCTT+TGG | + | chr6.3:27588038-27588057 | MS.gene80355:CDS | 50.0% | |
| GGTTCCTCGACTATCACCAT+GGG | - | chr6.3:27588036-27588055 | None:intergenic | 50.0% | |
| TCGATGCGAGTTCATCGTCA+AGG | + | chr6.3:27587195-27587214 | MS.gene80355:CDS | 50.0% | |
| TGTAAGGACCGTTGCAATGC+AGG | - | chr6.3:27587885-27587904 | None:intergenic | 50.0% | |
| TGTTTCGCCTCAGCAATGTG+AGG | + | chr6.3:27587638-27587657 | MS.gene80355:CDS | 50.0% | |
| ! | ACTATCACCATGGGAAGCGT+AGG | - | chr6.3:27588027-27588046 | None:intergenic | 50.0% |
| !! | AGTTCTGAAGGTGGTAGTGG+TGG | + | chr6.3:27587537-27587556 | MS.gene80355:CDS | 50.0% |
| !! | GCAATGGGTTGAATCTCGCT+CGG | + | chr6.3:27588241-27588260 | MS.gene80355:CDS | 50.0% |
| !!! | GGTGAGGCTTTTGATAACGG+CGG | + | chr6.3:27587491-27587510 | MS.gene80355:CDS | 50.0% |
| AACTCATCCGTGTCAAGGGC+CGG | + | chr6.3:27587248-27587267 | MS.gene80355:CDS | 55.0% | |
| ACCTTCAGAACTCCCGTCCT+CGG | - | chr6.3:27587529-27587548 | None:intergenic | 55.0% | |
| CGCACGATCCTGCATTGCAA+CGG | + | chr6.3:27587874-27587893 | MS.gene80355:CDS | 55.0% | |
| CGTTGCAATGCAGGATCGTG+CGG | - | chr6.3:27587876-27587895 | None:intergenic | 55.0% | |
| CTGAAGCGAAAGGAGATCGG+CGG | + | chr6.3:27588428-27588447 | MS.gene80355:CDS | 55.0% | |
| GAAGAGACCTACGCTTCCCA+TGG | + | chr6.3:27588017-27588036 | MS.gene80355:CDS | 55.0% | |
| GCTTCCCATGGTGATAGTCG+AGG | + | chr6.3:27588029-27588048 | MS.gene80355:CDS | 55.0% | |
| GGCGGTGTCTTATATTGCCG+AGG | + | chr6.3:27587509-27587528 | MS.gene80355:CDS | 55.0% | |
| TGACCATCACCACCTTCCTC+CGG | - | chr6.3:27587405-27587424 | None:intergenic | 55.0% | |
| ! | AGGAACCTTTGGGGGATTGG+GGG | + | chr6.3:27588049-27588068 | MS.gene80355:CDS | 55.0% |
| ! | CGAGGAACCTTTGGGGGATT+GGG | + | chr6.3:27588047-27588066 | MS.gene80355:CDS | 55.0% |
| ! | CGATGAACTCGCATCGATCC+CGG | - | chr6.3:27587192-27587211 | None:intergenic | 55.0% |
| ! | GAGGAACCTTTGGGGGATTG+GGG | + | chr6.3:27588048-27588067 | MS.gene80355:CDS | 55.0% |
| ! | TCGAGGAACCTTTGGGGGAT+TGG | + | chr6.3:27588046-27588065 | MS.gene80355:CDS | 55.0% |
| !! | CTGAAGGTGGTAGTGGTGGA+AGG | + | chr6.3:27587541-27587560 | MS.gene80355:CDS | 55.0% |
| !! | GGGAGTTCTGAAGGTGGTAG+TGG | + | chr6.3:27587534-27587553 | MS.gene80355:CDS | 55.0% |
| !! | GTTCAAGGTGATGGTGGTGG+TGG | + | chr6.3:27587141-27587160 | MS.gene80355:CDS | 55.0% |
| !!! | GGTGGTGGTGGTGGTTCTTT+TGG | + | chr6.3:27587153-27587172 | MS.gene80355:CDS | 55.0% |
| !!! | GGTGGTGGTGGTTCTTTTGG+TGG | + | chr6.3:27587156-27587175 | MS.gene80355:CDS | 55.0% |
| AGTTCCCCCCAATCCCCCAA+AGG | - | chr6.3:27588057-27588076 | None:intergenic | 60.0% | |
| GAAGATGAGCCGGAGGAAGG+TGG | + | chr6.3:27587393-27587412 | MS.gene80355:CDS | 60.0% | |
| GAGGAAGATGAGCCGGAGGA+AGG | + | chr6.3:27587390-27587409 | MS.gene80355:CDS | 60.0% | |
| GAGGGAAAAAGGGGTCGCCT+TGG | + | chr6.3:27587442-27587461 | MS.gene80355:CDS | 60.0% | |
| GAGTGAGGAAGATGAGCCGG+AGG | + | chr6.3:27587386-27587405 | MS.gene80355:CDS | 60.0% | |
| GGACAGACGGTCCAACCAGA+TGG | + | chr6.3:27588173-27588192 | MS.gene80355:CDS | 60.0% | |
| GGGGAGTGAGGAAGATGAGC+CGG | + | chr6.3:27587383-27587402 | MS.gene80355:CDS | 60.0% | |
| GGTTGGACCGTCTGTCCATG+AGG | - | chr6.3:27588170-27588189 | None:intergenic | 60.0% | |
| GTCCACTTCACACGCTGCCA+AGG | - | chr6.3:27587462-27587481 | None:intergenic | 60.0% | |
| ! | GGAACCTTTGGGGGATTGGG+GGG | + | chr6.3:27588050-27588069 | MS.gene80355:CDS | 60.0% |
| !! | CAAGGTGATGGTGGTGGTGG+TGG | + | chr6.3:27587144-27587163 | MS.gene80355:CDS | 60.0% |
| !! | GAGGACGGGAGTTCTGAAGG+TGG | + | chr6.3:27587528-27587547 | MS.gene80355:CDS | 60.0% |
| CGCCTTGGCAGCGTGTGAAG+TGG | + | chr6.3:27587457-27587476 | MS.gene80355:CDS | 65.0% | |
| GAGCCGGAGGAAGGTGGTGA+TGG | + | chr6.3:27587399-27587418 | MS.gene80355:CDS | 65.0% | |
| TCATCCGTGTCAAGGGCCGG+CGG | + | chr6.3:27587251-27587270 | MS.gene80355:CDS | 65.0% | |
| ! | GCCGAGGACGGGAGTTCTGA+AGG | + | chr6.3:27587525-27587544 | MS.gene80355:CDS | 65.0% |
| AGCACCGCCGGCCCTTGACA+CGG | - | chr6.3:27587258-27587277 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 27587111 | 27588466 | 27587111 | ID=MS.gene80355 |
| chr6.3 | mRNA | 27587111 | 27588466 | 27587111 | ID=MS.gene80355.t1;Parent=MS.gene80355 |
| chr6.3 | exon | 27587111 | 27588466 | 27587111 | ID=MS.gene80355.t1.exon1;Parent=MS.gene80355.t1 |
| chr6.3 | CDS | 27587111 | 27588466 | 27587111 | ID=cds.MS.gene80355.t1;Parent=MS.gene80355.t1 |
| Gene Sequence |
| Protein sequence |
