Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81085.t1 | XP_013444537.1 | 85.3 | 68 | 10 | 0 | 1 | 68 | 56 | 123 | 8.00E-23 | 115.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81085.t1 | Q8LCA1 | 58.1 | 74 | 25 | 2 | 1 | 68 | 54 | 127 | 3.4e-14 | 78.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81085.t1 | I3SWC2 | 85.3 | 68 | 10 | 0 | 1 | 68 | 56 | 123 | 5.8e-23 | 115.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049173 | MS.gene81085 | 0.809796 | 1.58E-50 | -1.69E-46 |
| MS.gene049217 | MS.gene81085 | 0.805095 | 1.57E-49 | -1.69E-46 |
| MS.gene049285 | MS.gene81085 | 0.804444 | 2.15E-49 | -1.69E-46 |
| MS.gene049320 | MS.gene81085 | 0.811506 | 6.77E-51 | -1.69E-46 |
| MS.gene049614 | MS.gene81085 | 0.820792 | 5.69E-53 | -1.69E-46 |
| MS.gene049661 | MS.gene81085 | 0.809852 | 1.54E-50 | -1.69E-46 |
| MS.gene049805 | MS.gene81085 | 0.813279 | 2.77E-51 | -1.69E-46 |
| MS.gene050076 | MS.gene81085 | 0.801708 | 7.93E-49 | -1.69E-46 |
| MS.gene050333 | MS.gene81085 | 0.806218 | 9.15E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene81085 | 0.817367 | 3.42E-52 | -1.69E-46 |
| MS.gene05053 | MS.gene81085 | 0.80006 | 1.72E-48 | -1.69E-46 |
| MS.gene050767 | MS.gene81085 | 0.842221 | 2.95E-58 | -1.69E-46 |
| MS.gene050770 | MS.gene81085 | 0.844449 | 7.51E-59 | -1.69E-46 |
| MS.gene050772 | MS.gene81085 | 0.828861 | 7.10E-55 | -1.69E-46 |
| MS.gene05100 | MS.gene81085 | 0.803826 | 2.90E-49 | -1.69E-46 |
| MS.gene05105 | MS.gene81085 | 0.801487 | 8.80E-49 | -1.69E-46 |
| MS.gene051228 | MS.gene81085 | 0.83519 | 1.94E-56 | -1.69E-46 |
| MS.gene051810 | MS.gene81085 | 0.833879 | 4.14E-56 | -1.69E-46 |
| MS.gene051858 | MS.gene81085 | 0.813143 | 2.97E-51 | -1.69E-46 |
| MS.gene052271 | MS.gene81085 | 0.854575 | 1.12E-61 | -1.69E-46 |
| MS.gene052290 | MS.gene81085 | 0.85446 | 1.21E-61 | -1.69E-46 |
| MS.gene052379 | MS.gene81085 | 0.816996 | 4.15E-52 | -1.69E-46 |
| MS.gene052421 | MS.gene81085 | 0.800638 | 1.31E-48 | -1.69E-46 |
| MS.gene052615 | MS.gene81085 | 0.821487 | 3.93E-53 | -1.69E-46 |
| MS.gene053009 | MS.gene81085 | 0.843573 | 1.29E-58 | -1.69E-46 |
| MS.gene053103 | MS.gene81085 | 0.829833 | 4.13E-55 | -1.69E-46 |
| MS.gene053104 | MS.gene81085 | 0.809922 | 1.49E-50 | -1.69E-46 |
| MS.gene053209 | MS.gene81085 | 0.800948 | 1.13E-48 | -1.69E-46 |
| MS.gene053213 | MS.gene81085 | 0.818801 | 1.62E-52 | -1.69E-46 |
| MS.gene053286 | MS.gene81085 | 0.806881 | 6.63E-50 | -1.69E-46 |
| MS.gene053322 | MS.gene81085 | 0.839309 | 1.71E-57 | -1.69E-46 |
| MS.gene053632 | MS.gene81085 | 0.833959 | 3.95E-56 | -1.69E-46 |
| MS.gene053912 | MS.gene81085 | 0.800075 | 1.71E-48 | -1.69E-46 |
| MS.gene053916 | MS.gene81085 | 0.80087 | 1.18E-48 | -1.69E-46 |
| MS.gene054376 | MS.gene81085 | 0.814777 | 1.30E-51 | -1.69E-46 |
| MS.gene054600 | MS.gene81085 | 0.803965 | 2.71E-49 | -1.69E-46 |
| MS.gene054642 | MS.gene81085 | 0.820356 | 7.16E-53 | -1.69E-46 |
| MS.gene054802 | MS.gene81085 | 0.804878 | 1.75E-49 | -1.69E-46 |
| MS.gene054803 | MS.gene81085 | 0.804157 | 2.47E-49 | -1.69E-46 |
| MS.gene054804 | MS.gene81085 | 0.808337 | 3.25E-50 | -1.69E-46 |
| MS.gene05505 | MS.gene81085 | 0.826905 | 2.10E-54 | -1.69E-46 |
| MS.gene055212 | MS.gene81085 | 0.808284 | 3.34E-50 | -1.69E-46 |
| MS.gene055254 | MS.gene81085 | 0.828805 | 7.33E-55 | -1.69E-46 |
| MS.gene055397 | MS.gene81085 | 0.837536 | 4.91E-57 | -1.69E-46 |
| MS.gene055412 | MS.gene81085 | 0.819816 | 9.52E-53 | -1.69E-46 |
| MS.gene055499 | MS.gene81085 | 0.842621 | 2.31E-58 | -1.69E-46 |
| MS.gene055647 | MS.gene81085 | 0.816006 | 6.91E-52 | -1.69E-46 |
| MS.gene055722 | MS.gene81085 | 0.830432 | 2.95E-55 | -1.69E-46 |
| MS.gene05592 | MS.gene81085 | 0.826021 | 3.41E-54 | -1.69E-46 |
| MS.gene056374 | MS.gene81085 | 0.802498 | 5.45E-49 | -1.69E-46 |
| MS.gene056609 | MS.gene81085 | 0.82293 | 1.82E-53 | -1.69E-46 |
| MS.gene056657 | MS.gene81085 | 0.818453 | 1.94E-52 | -1.69E-46 |
| MS.gene057129 | MS.gene81085 | 0.847017 | 1.51E-59 | -1.69E-46 |
| MS.gene057416 | MS.gene81085 | 0.830039 | 3.68E-55 | -1.69E-46 |
| MS.gene058419 | MS.gene81085 | 0.805671 | 1.19E-49 | -1.69E-46 |
| MS.gene058723 | MS.gene81085 | 0.824295 | 8.72E-54 | -1.69E-46 |
| MS.gene059029 | MS.gene81085 | 0.808086 | 3.68E-50 | -1.69E-46 |
| MS.gene059688 | MS.gene81085 | 0.804 | 2.66E-49 | -1.69E-46 |
| MS.gene059692 | MS.gene81085 | 0.809514 | 1.82E-50 | -1.69E-46 |
| MS.gene059695 | MS.gene81085 | 0.807511 | 4.88E-50 | -1.69E-46 |
| MS.gene059711 | MS.gene81085 | 0.840068 | 1.09E-57 | -1.69E-46 |
| MS.gene059718 | MS.gene81085 | 0.804168 | 2.46E-49 | -1.69E-46 |
| MS.gene059902 | MS.gene81085 | 0.813422 | 2.58E-51 | -1.69E-46 |
| MS.gene059960 | MS.gene81085 | 0.829232 | 5.77E-55 | -1.69E-46 |
| MS.gene060804 | MS.gene81085 | 0.810211 | 1.29E-50 | -1.69E-46 |
| MS.gene060818 | MS.gene81085 | 0.820259 | 7.54E-53 | -1.69E-46 |
| MS.gene061041 | MS.gene81085 | 0.838606 | 2.60E-57 | -1.69E-46 |
| MS.gene061200 | MS.gene81085 | 0.83928 | 1.74E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATTGCTCGTGATGTTA+TGG | 0.377640 | 8.4:+70875272 | MS.gene81085:CDS |
| TGCTCGTGATGTTATGGTTA+TGG | 0.415312 | 8.4:+70875278 | MS.gene81085:CDS |
| TAGCTAGTGTATTTGCACTA+TGG | 0.434144 | 8.4:+70875518 | MS.gene81085:CDS |
| TTTGCACTATGGAGCTCAGC+TGG | 0.465802 | 8.4:+70875529 | MS.gene81085:CDS |
| GATGTTATGGTTATGGCAAC+AGG | 0.470204 | 8.4:+70875285 | MS.gene81085:CDS |
| TGAAGATTCTTAACAAACTC+AGG | 0.486308 | 8.4:-70875342 | None:intergenic |
| TCAGTTGAGTCAACTTCTGT+TGG | 0.503744 | 8.4:-70875315 | None:intergenic |
| TAAATATGCAGTGGGTTCAT+TGG | 0.505381 | 8.4:+70875492 | MS.gene81085:CDS |
| TTTGATGATAAATATGCAGT+GGG | 0.562064 | 8.4:+70875484 | MS.gene81085:CDS |
| ATTTGATGATAAATATGCAG+TGG | 0.570116 | 8.4:+70875483 | MS.gene81085:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAAATAACATAATTCT+AGG | - | chr8.4:70875442-70875461 | None:intergenic | 10.0% |
| !!! | AATTATGTTATTTATTTTGT+TGG | + | chr8.4:70875444-70875463 | MS.gene81085:intron | 10.0% |
| !! | TCAATAATAAACATTAAGAA+GGG | - | chr8.4:70875419-70875438 | None:intergenic | 15.0% |
| !! | GTCAATAATAAACATTAAGA+AGG | - | chr8.4:70875420-70875439 | None:intergenic | 20.0% |
| ! | TAGGTTGTTAGAAATTGTAA+TGG | + | chr8.4:70875382-70875401 | MS.gene81085:intron | 25.0% |
| !! | ATTTGATGATAAATATGCAG+TGG | + | chr8.4:70875483-70875502 | MS.gene81085:CDS | 25.0% |
| !! | TTTGATGATAAATATGCAGT+GGG | + | chr8.4:70875484-70875503 | MS.gene81085:CDS | 25.0% |
| TGAAGATTCTTAACAAACTC+AGG | - | chr8.4:70875345-70875364 | None:intergenic | 30.0% | |
| ! | TATTTTGTTGGCATCATAGT+GGG | + | chr8.4:70875456-70875475 | MS.gene81085:intron | 30.0% |
| !! | TTATTTTGTTGGCATCATAG+TGG | + | chr8.4:70875455-70875474 | MS.gene81085:intron | 30.0% |
| AAAGATTGCTCGTGATGTTA+TGG | + | chr8.4:70875272-70875291 | MS.gene81085:CDS | 35.0% | |
| CAAGAAGCTGTATGATACAT+AGG | + | chr8.4:70875363-70875382 | MS.gene81085:intron | 35.0% | |
| TAGCTAGTGTATTTGCACTA+TGG | + | chr8.4:70875518-70875537 | MS.gene81085:CDS | 35.0% | |
| ! | TAAATATGCAGTGGGTTCAT+TGG | + | chr8.4:70875492-70875511 | MS.gene81085:CDS | 35.0% |
| GATGTTATGGTTATGGCAAC+AGG | + | chr8.4:70875285-70875304 | MS.gene81085:CDS | 40.0% | |
| TCAGTTGAGTCAACTTCTGT+TGG | - | chr8.4:70875318-70875337 | None:intergenic | 40.0% | |
| TGCTCGTGATGTTATGGTTA+TGG | + | chr8.4:70875278-70875297 | MS.gene81085:CDS | 40.0% | |
| !!! | GAGCTCAGCTGGTTTTATAT+CGG | + | chr8.4:70875540-70875559 | MS.gene81085:CDS | 40.0% |
| TTTGCACTATGGAGCTCAGC+TGG | + | chr8.4:70875529-70875548 | MS.gene81085:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 70875268 | 70875603 | 70875268 | ID=MS.gene81085 |
| chr8.4 | mRNA | 70875268 | 70875603 | 70875268 | ID=MS.gene81085.t1;Parent=MS.gene81085 |
| chr8.4 | exon | 70875268 | 70875371 | 70875268 | ID=MS.gene81085.t1.exon1;Parent=MS.gene81085.t1 |
| chr8.4 | CDS | 70875268 | 70875371 | 70875268 | ID=cds.MS.gene81085.t1;Parent=MS.gene81085.t1 |
| chr8.4 | exon | 70875475 | 70875603 | 70875475 | ID=MS.gene81085.t1.exon2;Parent=MS.gene81085.t1 |
| chr8.4 | CDS | 70875475 | 70875603 | 70875475 | ID=cds.MS.gene81085.t1;Parent=MS.gene81085.t1 |
| Gene Sequence |
| Protein sequence |
