AlfalfaGEDB | MS.gene05053

M. sativa cultivar XinJiangDaYe / MS.gene05053

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0008150 GO:0008152 GO:0009521 GO:0009523 GO:0009654 GO:0009987 GO:0015979 GO:0019898 GO:0032991 GO:0043167 GO:0043169 GO:0044237 GO:0046872 GO:0098796 GO:0110165 GO:1902494 GO:1990204
PFAM: PF05757.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05053.t1	XP_003600596.2	94.6	166	9	0	1	166	12	177	3.40E-82	314.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05053.t1	Q2V4B2	40.8	152	83	4	21	166	37	187	2.2e-21	103.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05053.t1	G7J7V3	94.6	166	9	0	1	166	12	177	2.4e-82	314.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049661	MS.gene05053	0.81809	2.35E-52	-1.69E-46
MS.gene049662	MS.gene05053	0.817802	2.73E-52	-1.69E-46
MS.gene05053	MS.gene050767	0.801956	7.05E-49	-1.69E-46
MS.gene05053	MS.gene050770	0.802298	6.00E-49	-1.69E-46
MS.gene05053	MS.gene052078	0.800677	1.29E-48	-1.69E-46
MS.gene05053	MS.gene052290	0.818583	1.82E-52	-1.69E-46
MS.gene05053	MS.gene052310	0.807516	4.87E-50	-1.69E-46
MS.gene05053	MS.gene054376	0.811711	6.11E-51	-1.69E-46
MS.gene05053	MS.gene054803	0.800864	1.18E-48	-1.69E-46
MS.gene05053	MS.gene054804	0.801231	9.92E-49	-1.69E-46
MS.gene05053	MS.gene055254	0.802564	5.29E-49	-1.69E-46
MS.gene05053	MS.gene055277	0.82114	4.73E-53	-1.69E-46
MS.gene05053	MS.gene05702	0.804298	2.31E-49	-1.69E-46
MS.gene05053	MS.gene058660	0.816212	6.21E-52	-1.69E-46
MS.gene05053	MS.gene059597	0.803014	4.27E-49	-1.69E-46
MS.gene05053	MS.gene059894	0.800244	1.58E-48	-1.69E-46
MS.gene05053	MS.gene061049	0.816758	4.69E-52	-1.69E-46
MS.gene05053	MS.gene061284	0.805373	1.38E-49	-1.69E-46
MS.gene05053	MS.gene061470	0.801116	1.05E-48	-1.69E-46
MS.gene05053	MS.gene061788	0.815354	9.65E-52	-1.69E-46
MS.gene05053	MS.gene062883	0.801591	8.38E-49	-1.69E-46
MS.gene05053	MS.gene063074	0.80403	2.63E-49	-1.69E-46
MS.gene05053	MS.gene065137	0.816015	6.88E-52	-1.69E-46
MS.gene05053	MS.gene065138	0.818575	1.82E-52	-1.69E-46
MS.gene05053	MS.gene066053	0.826578	2.51E-54	-1.69E-46
MS.gene05053	MS.gene06681	0.802238	6.17E-49	-1.69E-46
MS.gene05053	MS.gene069124	0.803607	3.22E-49	-1.69E-46
MS.gene05053	MS.gene069859	0.814665	1.37E-51	-1.69E-46
MS.gene05053	MS.gene070666	0.821555	3.79E-53	-1.69E-46
MS.gene05053	MS.gene071613	0.809877	1.52E-50	-1.69E-46
MS.gene05053	MS.gene072196	0.813304	2.74E-51	-1.69E-46
MS.gene05053	MS.gene072568	0.80966	1.70E-50	-1.69E-46
MS.gene05053	MS.gene072571	0.809903	1.50E-50	-1.69E-46
MS.gene05053	MS.gene073925	0.819812	9.54E-53	-1.69E-46
MS.gene05053	MS.gene073926	0.811462	6.92E-51	-1.69E-46
MS.gene05053	MS.gene073963	0.820938	5.26E-53	-1.69E-46
MS.gene05053	MS.gene07619	0.811304	7.49E-51	-1.69E-46
MS.gene05053	MS.gene09357	0.817609	3.02E-52	-1.69E-46
MS.gene05053	MS.gene20931	0.815104	1.10E-51	-1.69E-46
MS.gene05053	MS.gene21116	0.805566	1.25E-49	-1.69E-46
MS.gene05053	MS.gene21530	0.803344	3.65E-49	-1.69E-46
MS.gene05053	MS.gene22206	0.82473	6.89E-54	-1.69E-46
MS.gene05053	MS.gene22209	0.82488	6.35E-54	-1.69E-46
MS.gene05053	MS.gene22222	0.808756	2.65E-50	-1.69E-46
MS.gene05053	MS.gene22292	0.800698	1.28E-48	-1.69E-46
MS.gene05053	MS.gene23002	0.822359	2.47E-53	-1.69E-46
MS.gene05053	MS.gene23137	0.803746	3.01E-49	-1.69E-46
MS.gene05053	MS.gene23141	0.802741	4.86E-49	-1.69E-46
MS.gene05053	MS.gene23271	0.820683	6.02E-53	-1.69E-46
MS.gene05053	MS.gene23328	0.826274	2.97E-54	-1.69E-46
MS.gene05053	MS.gene23923	0.808803	2.59E-50	-1.69E-46
MS.gene05053	MS.gene26340	0.819627	1.05E-52	-1.69E-46
MS.gene05053	MS.gene27725	0.825621	4.24E-54	-1.69E-46
MS.gene05053	MS.gene28067	0.814299	1.65E-51	-1.69E-46
MS.gene05053	MS.gene30766	0.813253	2.81E-51	-1.69E-46
MS.gene05053	MS.gene32274	0.803605	3.22E-49	-1.69E-46
MS.gene05053	MS.gene33855	0.801617	8.27E-49	-1.69E-46
MS.gene05053	MS.gene33873	0.800878	1.17E-48	-1.69E-46
MS.gene05053	MS.gene34231	0.808296	3.32E-50	-1.69E-46
MS.gene05053	MS.gene34843	0.805826	1.11E-49	-1.69E-46
MS.gene05053	MS.gene35738	0.81922	1.30E-52	-1.69E-46
MS.gene05053	MS.gene36610	0.811563	6.58E-51	-1.69E-46
MS.gene05053	MS.gene36648	0.81676	4.68E-52	-1.69E-46
MS.gene05053	MS.gene36722	0.801922	7.16E-49	-1.69E-46
MS.gene05053	MS.gene38579	0.826982	2.01E-54	-1.69E-46
MS.gene05053	MS.gene38600	0.828327	9.56E-55	-1.69E-46
MS.gene05053	MS.gene40233	0.811482	6.85E-51	-1.69E-46
MS.gene05053	MS.gene42992	0.805509	1.29E-49	-1.69E-46
MS.gene05053	MS.gene43403	0.80996	1.46E-50	-1.69E-46
MS.gene05053	MS.gene46602	0.807112	5.93E-50	-1.69E-46
MS.gene05053	MS.gene46942	0.800125	1.67E-48	-1.69E-46
MS.gene05053	MS.gene47537	0.805498	1.30E-49	-1.69E-46
MS.gene05053	MS.gene48509	0.82535	4.92E-54	-1.69E-46
MS.gene05053	MS.gene49257	0.82064	6.16E-53	-1.69E-46
MS.gene05053	MS.gene50731	0.810259	1.26E-50	-1.69E-46
MS.gene05053	MS.gene52238	0.826153	3.17E-54	-1.69E-46
MS.gene05053	MS.gene52568	0.809927	1.49E-50	-1.69E-46
MS.gene05053	MS.gene53332	0.803528	3.34E-49	-1.69E-46
MS.gene05053	MS.gene53556	0.803085	4.12E-49	-1.69E-46
MS.gene05053	MS.gene54700	0.809679	1.68E-50	-1.69E-46
MS.gene05053	MS.gene55769	0.817555	3.10E-52	-1.69E-46
MS.gene05053	MS.gene57121	0.809604	1.74E-50	-1.69E-46
MS.gene05053	MS.gene57299	0.814961	1.18E-51	-1.69E-46
MS.gene05053	MS.gene57547	0.81917	1.34E-52	-1.69E-46
MS.gene05053	MS.gene58160	0.822657	2.11E-53	-1.69E-46
MS.gene05053	MS.gene58162	0.815201	1.04E-51	-1.69E-46
MS.gene05053	MS.gene58210	0.808465	3.06E-50	-1.69E-46
MS.gene05053	MS.gene59730	0.819771	9.75E-53	-1.69E-46
MS.gene05053	MS.gene59950	0.813281	2.77E-51	-1.69E-46
MS.gene05053	MS.gene62645	0.817014	4.11E-52	-1.69E-46
MS.gene05053	MS.gene62901	0.802126	6.51E-49	-1.69E-46
MS.gene05053	MS.gene63280	0.827367	1.63E-54	-1.69E-46
MS.gene05053	MS.gene63957	0.812209	4.76E-51	-1.69E-46
MS.gene05053	MS.gene64414	0.805926	1.05E-49	-1.69E-46
MS.gene05053	MS.gene64922	0.805231	1.48E-49	-1.69E-46
MS.gene05053	MS.gene65851	0.811607	6.43E-51	-1.69E-46
MS.gene05053	MS.gene66260	0.805287	1.44E-49	-1.69E-46
MS.gene05053	MS.gene66262	0.809612	1.74E-50	-1.69E-46
MS.gene05053	MS.gene67066	0.806827	6.81E-50	-1.69E-46
MS.gene05053	MS.gene67463	0.812221	4.73E-51	-1.69E-46
MS.gene05053	MS.gene68129	0.800917	1.15E-48	-1.69E-46
MS.gene05053	MS.gene68135	0.802884	4.54E-49	-1.69E-46
MS.gene05053	MS.gene68178	0.827217	1.77E-54	-1.69E-46
MS.gene05053	MS.gene68723	0.802584	5.23E-49	-1.69E-46
MS.gene05053	MS.gene68729	0.80096	1.13E-48	-1.69E-46
MS.gene05053	MS.gene69083	0.803035	4.22E-49	-1.69E-46
MS.gene05053	MS.gene69798	0.802409	5.69E-49	-1.69E-46
MS.gene05053	MS.gene71024	0.81144	6.99E-51	-1.69E-46
MS.gene05053	MS.gene71330	0.808422	3.12E-50	-1.69E-46
MS.gene05053	MS.gene71604	0.831757	1.39E-55	-1.69E-46
MS.gene05053	MS.gene71829	0.806	1.02E-49	-1.69E-46
MS.gene05053	MS.gene72005	0.804095	2.55E-49	-1.69E-46
MS.gene05053	MS.gene72364	0.800373	1.49E-48	-1.69E-46
MS.gene05053	MS.gene74225	0.805299	1.43E-49	-1.69E-46
MS.gene05053	MS.gene75021	0.818734	1.68E-52	-1.69E-46
MS.gene05053	MS.gene75815	0.80285	4.61E-49	-1.69E-46
MS.gene05053	MS.gene76306	0.831023	2.11E-55	-1.69E-46
MS.gene05053	MS.gene77868	0.805054	1.61E-49	-1.69E-46
MS.gene05053	MS.gene79342	0.804208	2.41E-49	-1.69E-46
MS.gene05053	MS.gene80354	0.824851	6.45E-54	-1.69E-46
MS.gene05053	MS.gene81085	0.80006	1.72E-48	-1.69E-46
MS.gene05053	MS.gene83767	0.824134	9.52E-54	-1.69E-46
MS.gene05053	MS.gene85449	0.814414	1.56E-51	-1.69E-46
MS.gene05053	MS.gene85727	0.823609	1.26E-53	-1.69E-46
MS.gene05053	MS.gene86932	0.81821	2.21E-52	-1.69E-46
MS.gene05053	MS.gene87280	0.831076	2.05E-55	-1.69E-46
MS.gene05053	MS.gene87687	0.808643	2.80E-50	-1.69E-46
MS.gene05053	MS.gene91319	0.857217	1.90E-62	-1.69E-46
MS.gene05053	MS.gene91325	0.848904	4.55E-60	-1.69E-46
MS.gene05053	MS.gene92661	0.824539	7.64E-54	-1.69E-46
MS.gene05053	MS.gene92761	0.835799	1.36E-56	-1.69E-46
MS.gene05053	MS.gene92871	0.803711	3.06E-49	-1.69E-46
MS.gene05053	MS.gene94333	0.80134	9.43E-49	-1.69E-46
MS.gene05053	MS.gene95123	0.810637	1.04E-50	-1.69E-46
MS.gene05053	MS.gene95864	0.814816	1.27E-51	-1.69E-46
MS.gene05053	MS.gene96705	0.816176	6.33E-52	-1.69E-46
MS.gene05053	MS.gene96754	0.800819	1.20E-48	-1.69E-46
MS.gene05053	MS.gene96838	0.803469	3.43E-49	-1.69E-46
MS.gene05053	MS.gene96864	0.800625	1.32E-48	-1.69E-46
MS.gene05053	MS.gene97081	0.806374	8.48E-50	-1.69E-46
MS.gene05053	MS.gene99139	0.805833	1.10E-49	-1.69E-46
MS.gene05053	MS.gene99622	0.805845	1.10E-49	-1.69E-46
MS.gene05053	MS.gene99806	0.811898	5.56E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05053.t1	MTR_3g064070	94.578	166	9	0	1	166	12	177	3.82e-116	326
MS.gene05053.t1	MTR_8g005175	29.524	105	74	0	62	166	127	231	7.18e-12	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene05053.t1	AT2G01918	42.000	150	73	5	31	166	38	187	7.13e-30	108
MS.gene05053.t1	AT4G21280	31.852	135	85	2	39	166	89	223	7.23e-18	77.8
MS.gene05053.t1	AT4G21280	32.031	128	80	2	46	166	95	222	3.48e-17	75.9
MS.gene05053.t1	AT4G05180	29.688	128	83	2	46	166	102	229	9.95e-16	72.0
MS.gene05053.t1	AT4G05180	29.688	128	83	2	46	166	102	229	9.95e-16	72.0

Find 20 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGACACAACATGTGAATTT+TGG	0.139486	3.4:-65850788	None:intergenic
AATGCAAATGCATTTGATTT+TGG	0.161838	3.4:+65850914	MS.gene05053:CDS
GAAAATCAGTAGAAGAAATT+TGG	0.169295	3.4:+65850853	MS.gene05053:CDS
AACTGTGACCTTTCACTTCC+AGG	0.232695	3.4:-65851115	None:intergenic
AAAATCAGTAGAAGAAATTT+GGG	0.278260	3.4:+65850854	MS.gene05053:CDS
AATCTTTCTGTTGAGAATGT+TGG	0.372717	3.4:-65850823	None:intergenic
TCATAACGCTGCCACACTTC+AGG	0.379368	3.4:-65851329	None:intergenic
GCCTCTTCAACTGTTTGATC+AGG	0.386829	3.4:-65850947	None:intergenic
GTCATGGAAAATAGCACAAA+AGG	0.423780	3.4:+65851033	MS.gene05053:CDS
TTAGTGGAGAGTGCATGTTT+AGG	0.480583	3.4:+65850885	MS.gene05053:CDS
ACCATAATTCAAAACAAGCC+TGG	0.511434	3.4:+65851097	MS.gene05053:CDS
GAAGTGAAAGGTCACAGTTA+AGG	0.517020	3.4:+65851119	MS.gene05053:CDS
TGAGAATGTTGGAAATTACA+AGG	0.547178	3.4:-65850812	None:intergenic
ACCTGATCAAACAGTTGAAG+AGG	0.575452	3.4:+65850946	MS.gene05053:CDS
AAAACAAGCCTGGAAGTGAA+AGG	0.596015	3.4:+65851107	MS.gene05053:CDS
ATTTGTTAGAGTCAAAGTCA+TGG	0.608950	3.4:+65851017	MS.gene05053:CDS
ATCAAGATGGACCTGAAGTG+TGG	0.634230	3.4:+65851318	MS.gene05053:CDS
GCGTTATGAGAACATTGTTG+TGG	0.635212	3.4:+65851343	MS.gene05053:CDS
TATGCAGCAAGAGATCAAGA+TGG	0.661307	3.4:+65851305	MS.gene05053:CDS
AAATCAGTAGAAGAAATTTG+GGG	0.677600	3.4:+65850855	MS.gene05053:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTGATGTTTATTTTTGTAT+TGG	+	chr3.4:65851261-65851280	MS.gene05053:intron	15.0%
!!	AAAATCAGTAGAAGAAATTT+GGG	+	chr3.4:65850854-65850873	MS.gene05053:CDS	20.0%
!!!	TTTATTGTTTTTGTTGTGTA+AGG	+	chr3.4:65851223-65851242	MS.gene05053:intron	20.0%
!	AAATCAGTAGAAGAAATTTG+GGG	+	chr3.4:65850855-65850874	MS.gene05053:CDS	25.0%
!	GAAAATCAGTAGAAGAAATT+TGG	+	chr3.4:65850853-65850872	MS.gene05053:CDS	25.0%
!!!	AATGCAAATGCATTTGATTT+TGG	+	chr3.4:65850914-65850933	MS.gene05053:CDS	25.0%
	AATCTTTCTGTTGAGAATGT+TGG	-	chr3.4:65850826-65850845	None:intergenic	30.0%
	GGAAACTTTGTCTTAAAACT+AGG	+	chr3.4:65851182-65851201	MS.gene05053:intron	30.0%
	TCAACAATGTTACTAGAGTA+AGG	+	chr3.4:65851161-65851180	MS.gene05053:intron	30.0%
	TGAGAATGTTGGAAATTACA+AGG	-	chr3.4:65850815-65850834	None:intergenic	30.0%
!	ATTTGTTAGAGTCAAAGTCA+TGG	+	chr3.4:65851017-65851036	MS.gene05053:CDS	30.0%
!	TTGACACAACATGTGAATTT+TGG	-	chr3.4:65850791-65850810	None:intergenic	30.0%
!!	AATTTGGGGTTGATTTTTAG+TGG	+	chr3.4:65850869-65850888	MS.gene05053:CDS	30.0%
	ACCATAATTCAAAACAAGCC+TGG	+	chr3.4:65851097-65851116	MS.gene05053:CDS	35.0%
	GTCATGGAAAATAGCACAAA+AGG	+	chr3.4:65851033-65851052	MS.gene05053:CDS	35.0%
!!	AAGTTCAGCACTTTTGAAGA+AGG	+	chr3.4:65851066-65851085	MS.gene05053:CDS	35.0%
!!	TGCATTTGATTTTGGACTTG+TGG	+	chr3.4:65850922-65850941	MS.gene05053:CDS	35.0%
!!!	TCCAGGCTTGTTTTGAATTA+TGG	-	chr3.4:65851101-65851120	None:intergenic	35.0%
	AAAACAAGCCTGGAAGTGAA+AGG	+	chr3.4:65851107-65851126	MS.gene05053:CDS	40.0%
	ACCTGATCAAACAGTTGAAG+AGG	+	chr3.4:65850946-65850965	MS.gene05053:CDS	40.0%
	GAAGTGAAAGGTCACAGTTA+AGG	+	chr3.4:65851119-65851138	MS.gene05053:CDS	40.0%
	GCGTTATGAGAACATTGTTG+TGG	+	chr3.4:65851343-65851362	MS.gene05053:CDS	40.0%
	TATGCAGCAAGAGATCAAGA+TGG	+	chr3.4:65851305-65851324	MS.gene05053:CDS	40.0%
!	TTAGTGGAGAGTGCATGTTT+AGG	+	chr3.4:65850885-65850904	MS.gene05053:CDS	40.0%
	AACTGTGACCTTTCACTTCC+AGG	-	chr3.4:65851118-65851137	None:intergenic	45.0%
	ATCAAGATGGACCTGAAGTG+TGG	+	chr3.4:65851318-65851337	MS.gene05053:CDS	45.0%
	GCCTCTTCAACTGTTTGATC+AGG	-	chr3.4:65850950-65850969	None:intergenic	45.0%
	TCATAACGCTGCCACACTTC+AGG	-	chr3.4:65851332-65851351	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.4	gene	65850773	65851394	65850773	ID=MS.gene05053
chr3.4	mRNA	65850773	65851394	65850773	ID=MS.gene05053.t1;Parent=MS.gene05053
chr3.4	exon	65850773	65851177	65850773	ID=MS.gene05053.t1.exon1;Parent=MS.gene05053.t1
chr3.4	CDS	65850773	65851177	65850773	ID=cds.MS.gene05053.t1;Parent=MS.gene05053.t1
chr3.4	exon	65851299	65851394	65851299	ID=MS.gene05053.t1.exon2;Parent=MS.gene05053.t1
chr3.4	CDS	65851299	65851394	65851299	ID=cds.MS.gene05053.t1;Parent=MS.gene05053.t1

Gene Sequence

>MS.gene05053

ATGACACACTTGTTTCCAAAATTCACATGTTGTGTCAAACCTTGTAATTTCCAACATTCTCAACAGAAAGATTTGTCACTGAAAATCAGTAGAAGAAATTTGGGGTTGATTTTTAGTGGAGAGTGCATGTTTAGGACAGAGAATGCAAATGCATTTGATTTTGGACTTGTGGTACCTGATCAAACAGTTGAAGAGGCACAAAATGTAGTGAGAGTTCATGCACAAGATTTATTGCAAGTTAGAGATTTGTTAGAGTCAAAGTCATGGAAAATAGCACAAAAGGAATTGAGAAGAAGTTCAGCACTTTTGAAGAAGGATATATACACCATAATTCAAAACAAGCCTGGAAGTGAAAGGTCACAGTTAAGGAAACTCTATTCTACTCTCTTCAACAATGTTACTAGAGTAAGGAAACTTTGTCTTAAAACTAGGACACTTTGTCATGAAGAGTTTATTGTTTTTGTTGTGTAAGGTTTTATTACTTTTCAATTGATGTTTATTTTTGTATTGGTGAAAAATAATGCAGCTAGATTATGCAGCAAGAGATCAAGATGGACCTGAAGTGTGGCAGCGTTATGAGAACATTGTTGTGGCTGTGAATGAAATTCTATCAAGAATATAA

Protein sequence

>MS.gene05053.t1

MTHLFPKFTCCVKPCNFQHSQQKDLSLKISRRNLGLIFSGECMFRTENANAFDFGLVVPDQTVEEAQNVVRVHAQDLLQVRDLLESKSWKIAQKELRRSSALLKKDIYTIIQNKPGSERSQLRKLYSTLFNNVTRLDYAARDQDGPEVWQRYENIVVAVNEILSRI