AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene85312


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85312.t1 MTR_2g044920 96.154 104 4 0 4 107 63 166 5.01e-72 211
MS.gene85312.t1 MTR_2g044920 94.643 56 3 0 4 59 63 118 6.00e-34 114
MS.gene85312.t1 MTR_5g041290 35.922 103 62 2 4 105 64 163 1.40e-15 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene85312.t1 AT1G48350 81.731 104 19 0 4 107 67 170 3.63e-61 184
MS.gene85312.t1 AT1G14205 39.000 100 57 2 7 105 71 167 1.14e-14 66.2
MS.gene85312.t1 AT3G17626 71.053 38 11 0 54 91 10 47 8.33e-14 60.8

Find 28 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAAAATCCTGTCTGGAAAT+AGG 0.169851 2.4:+45473725 MS.gene85312:CDS
CACAGACAATCTTGGTCTTT+CGG 0.279312 2.4:-45472677 None:intergenic
ACAGACAATCTTGGTCTTTC+GGG 0.288767 2.4:-45472676 None:intergenic
CAAAATCCTGTCTGGAAATA+GGG 0.298158 2.4:+45473726 MS.gene85312:CDS
TTGGACAAAGAGATGCTTGT+TGG 0.418067 2.4:-45472707 None:intergenic
GCTTTAACACGGCCATGGTA+TGG 0.425794 2.4:-45473782 None:intergenic
TATCAGGAACTTACAATTGT+AGG 0.452312 2.4:-45472817 None:intergenic
AATCATTGCAAAATCCTGTC+TGG 0.467654 2.4:+45473718 MS.gene85312:CDS
TGTGATCCCTATTTCCAGAC+AGG 0.474164 2.4:-45473732 None:intergenic
GATCGAAACACAGACAATCT+TGG 0.494991 2.4:-45472685 None:intergenic
CTTTAACACGGCCATGGTAT+GGG 0.511001 2.4:-45473781 None:intergenic
GAAGAGTTGAACTTCACCTC+TGG 0.512553 2.4:+45472793 MS.gene85312:CDS
TCTTGGTGTCGTCAATCACT+TGG 0.549933 2.4:-45472726 None:intergenic
GCTGATGCAGCTCGCGAACA+TGG 0.565784 2.4:+45473809 MS.gene85312:CDS
ACAAAGGTAGCCTTTGACAG+AGG 0.569891 2.4:+45473752 MS.gene85312:CDS
TGGGTAACCACCTCTGTCAA+AGG 0.571658 2.4:-45473762 None:intergenic
TGAAGCAAGTGTATGCATCT+TGG 0.577835 2.4:-45472743 None:intergenic
GCTTCAGGAAGTAGCAAAGA+GGG 0.581508 2.4:+45473688 MS.gene85312:intron
TTCAGCTTCAACAATGCAGA+AGG 0.583407 2.4:+45472762 MS.gene85312:CDS
AAGGTAGCCTTTGACAGAGG+TGG 0.614954 2.4:+45473755 MS.gene85312:CDS
CAATAGCTTTAACACGGCCA+TGG 0.626600 2.4:-45473787 None:intergenic
CATCAGCAATAGCTTTAACA+CGG 0.638165 2.4:-45473793 None:intergenic
TGCTTCAGGAAGTAGCAAAG+AGG 0.655766 2.4:+45473687 MS.gene85312:intron
TCAGGAAGTAGCAAAGAGGG+TGG 0.657714 2.4:+45473691 MS.gene85312:intron
CAGGAAGTAGCAAAGAGGGT+GGG 0.658309 2.4:+45473692 MS.gene85312:intron
ACTTACAATTGTAGGACCAG+AGG 0.688517 2.4:-45472809 None:intergenic
AGAGGTGGTTACCCATACCA+TGG 0.695112 2.4:+45473770 MS.gene85312:CDS
TCTGGAAATAGGGATCACAA+AGG 0.730190 2.4:+45473736 MS.gene85312:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATTAGTTATTACCAT+TGG - chr2.4:45473275-45473294 None:intergenic 15.0%
!! TAATAAGTATTTAGGAAAAA+AGG - chr2.4:45472947-45472966 None:intergenic 15.0%
!!! ATGACTTTTAATAAGTATTT+AGG - chr2.4:45472955-45472974 None:intergenic 15.0%
!! TCTACGATTTAATAATGAAA+GGG - chr2.4:45473520-45473539 None:intergenic 20.0%
!! TTCTACGATTTAATAATGAA+AGG - chr2.4:45473521-45473540 None:intergenic 20.0%
!!! AGGAATTTTTATTATCATCA+CGG - chr2.4:45472927-45472946 None:intergenic 20.0%
!!! TTTAGTTTTATGCATTGTTA+TGG - chr2.4:45472985-45473004 None:intergenic 20.0%
! ATATACTCAAGGAAACTAAA+AGG - chr2.4:45473207-45473226 None:intergenic 25.0%
! ATATGTTCAGTAGCTATTAT+GGG - chr2.4:45473333-45473352 None:intergenic 25.0%
! ATGAAGTAGTAATAATATGC+AGG - chr2.4:45473064-45473083 None:intergenic 25.0%
! TTAGTTATTACCATTGGAAA+TGG - chr2.4:45473269-45473288 None:intergenic 25.0%
! TTTATTATCATCACGGAATA+GGG - chr2.4:45472920-45472939 None:intergenic 25.0%
!! TTCATTTTCATTGATCACAA+GGG + chr2.4:45473305-45473324 MS.gene85312:intron 25.0%
!! TTTCATTTTCATTGATCACA+AGG + chr2.4:45473304-45473323 MS.gene85312:intron 25.0%
!! TTTTATTATCATCACGGAAT+AGG - chr2.4:45472921-45472940 None:intergenic 25.0%
!!! ATTCCAGGACTTTTTTTTTA+AGG - chr2.4:45473413-45473432 None:intergenic 25.0%
AAAACTAAACACTGATCACA+GGG + chr2.4:45472996-45473015 MS.gene85312:intron 30.0%
AATAAGTTCAACCAAGCAAA+TGG + chr2.4:45473438-45473457 MS.gene85312:intron 30.0%
ATGCTTCTGTAATATACTCA+AGG - chr2.4:45473218-45473237 None:intergenic 30.0%
ATTTGCTTGGTTGAACTTAT+TGG - chr2.4:45473439-45473458 None:intergenic 30.0%
CATATGTTCAGTAGCTATTA+TGG - chr2.4:45473334-45473353 None:intergenic 30.0%
GTTCCTTAAAAAAAAAGTCC+TGG + chr2.4:45473407-45473426 MS.gene85312:intron 30.0%
TAAAACTAAACACTGATCAC+AGG + chr2.4:45472995-45473014 MS.gene85312:intron 30.0%
TAATAGCTACTGAACATATG+AGG + chr2.4:45473334-45473353 MS.gene85312:intron 30.0%
TATCAGGAACTTACAATTGT+AGG - chr2.4:45472820-45472839 None:intergenic 30.0%
TATGTTCAGTAGCTATTATG+GGG - chr2.4:45473332-45473351 None:intergenic 30.0%
TTAGCCAAAAGGTTTATTCT+TGG + chr2.4:45473132-45473151 MS.gene85312:intron 30.0%
TTATTATCATCACGGAATAG+GGG - chr2.4:45472919-45472938 None:intergenic 30.0%
TTCTTTACCTATTAGCCAAA+AGG + chr2.4:45473121-45473140 MS.gene85312:intron 30.0%
TTGACATAGAAGTTAAACCA+TGG + chr2.4:45473170-45473189 MS.gene85312:intron 30.0%
! AAGTTCTTTTCCATTTCCAA+TGG + chr2.4:45473256-45473275 MS.gene85312:intron 30.0%
! AATTTTCGTTTACCGAATGA+CGG + chr2.4:45473642-45473661 MS.gene85312:intron 30.0%
! AGAAAGTTCTAATTGAGCAT+AGG + chr2.4:45473537-45473556 MS.gene85312:intron 30.0%
! ATACAACAAGCTTTTAAGCA+AGG - chr2.4:45473606-45473625 None:intergenic 30.0%
! CTATCCAAGAATAAACCTTT+TGG - chr2.4:45473139-45473158 None:intergenic 30.0%
! TACAACAAGCTTTTAAGCAA+GGG - chr2.4:45473605-45473624 None:intergenic 30.0%
!! CAACATCTTTCTTTGAAATC+TGG - chr2.4:45473040-45473059 None:intergenic 30.0%
!! GAATAAACCTTTTGGCTAAT+AGG - chr2.4:45473131-45473150 None:intergenic 30.0%
AAAAGAACTTCAGGATGCAA+AGG - chr2.4:45473246-45473265 None:intergenic 35.0%
AAAGAACTTCAGGATGCAAA+GGG - chr2.4:45473245-45473264 None:intergenic 35.0%
AATCATTGCAAAATCCTGTC+TGG + chr2.4:45473718-45473737 MS.gene85312:CDS 35.0%
CAAAATCCTGTCTGGAAATA+GGG + chr2.4:45473726-45473745 MS.gene85312:CDS 35.0%
CAATACAGTTTGAAAGGATC+AGG - chr2.4:45473669-45473688 None:intergenic 35.0%
CTGAAGCAATACAGTTTGAA+AGG - chr2.4:45473675-45473694 None:intergenic 35.0%
CTTTCAAACTGTATTGCTTC+AGG + chr2.4:45473673-45473692 MS.gene85312:intron 35.0%
TAATTTAACTCCTGCCAGAT+TGG + chr2.4:45472862-45472881 MS.gene85312:intron 35.0%
TAGAAGTTAAACCATGGAGA+AGG + chr2.4:45473176-45473195 MS.gene85312:intron 35.0%
TGAACTTATTGGTAGATTCC+AGG - chr2.4:45473428-45473447 None:intergenic 35.0%
TGGAAATGGAAAAGAACTTC+AGG - chr2.4:45473255-45473274 None:intergenic 35.0%
! TTTTAAGCAAGGGAGATTGA+GGG - chr2.4:45473595-45473614 None:intergenic 35.0%
!! CATCAGCAATAGCTTTAACA+CGG - chr2.4:45473796-45473815 None:intergenic 35.0%
ACTTACAATTGTAGGACCAG+AGG - chr2.4:45472812-45472831 None:intergenic 40.0%
ATATCGTGGATTTCTACGAC+AGG + chr2.4:45473384-45473403 MS.gene85312:intron 40.0%
GATCGAAACACAGACAATCT+TGG - chr2.4:45472688-45472707 None:intergenic 40.0%
GCAAAATCCTGTCTGGAAAT+AGG + chr2.4:45473725-45473744 MS.gene85312:CDS 40.0%
GTTGTTGAAGACCATTTGCT+TGG - chr2.4:45473452-45473471 None:intergenic 40.0%
TATCGTGGATTTCTACGACA+GGG + chr2.4:45473385-45473404 MS.gene85312:intron 40.0%
TCTGGAAATAGGGATCACAA+AGG + chr2.4:45473736-45473755 MS.gene85312:CDS 40.0%
TGAAGCAAGTGTATGCATCT+TGG - chr2.4:45472746-45472765 None:intergenic 40.0%
TGTCGTAGAAATCCACGATA+TGG - chr2.4:45473385-45473404 None:intergenic 40.0%
TTCAGCTTCAACAATGCAGA+AGG + chr2.4:45472762-45472781 MS.gene85312:CDS 40.0%
TTGGACAAAGAGATGCTTGT+TGG - chr2.4:45472710-45472729 None:intergenic 40.0%
! ACAGACAATCTTGGTCTTTC+GGG - chr2.4:45472679-45472698 None:intergenic 40.0%
! CACAGACAATCTTGGTCTTT+CGG - chr2.4:45472680-45472699 None:intergenic 40.0%
! CTTTTAAGCAAGGGAGATTG+AGG - chr2.4:45473596-45473615 None:intergenic 40.0%
! GAGAAACGTAAAAGCGTATC+AGG - chr2.4:45472836-45472855 None:intergenic 40.0%
AAAAGGTTGCTCCTTCTCCA+TGG - chr2.4:45473190-45473209 None:intergenic 45.0%
AAAGGATCAGGACCGTCATT+CGG - chr2.4:45473657-45473676 None:intergenic 45.0%
ACAAAGGTAGCCTTTGACAG+AGG + chr2.4:45473752-45473771 MS.gene85312:CDS 45.0%
ACACAACACTGAGTCCAATC+TGG - chr2.4:45472879-45472898 None:intergenic 45.0%
CTTTAACACGGCCATGGTAT+GGG - chr2.4:45473784-45473803 None:intergenic 45.0%
GAAGAGTTGAACTTCACCTC+TGG + chr2.4:45472793-45472812 MS.gene85312:CDS 45.0%
GCTTCAGGAAGTAGCAAAGA+GGG + chr2.4:45473688-45473707 MS.gene85312:intron 45.0%
TGCTTCAGGAAGTAGCAAAG+AGG + chr2.4:45473687-45473706 MS.gene85312:intron 45.0%
TGTGATCCCTATTTCCAGAC+AGG - chr2.4:45473735-45473754 None:intergenic 45.0%
! GCAATGACTTGAAAGCACAG+TGG + chr2.4:45473356-45473375 MS.gene85312:intron 45.0%
!! CAATAGCTTTAACACGGCCA+TGG - chr2.4:45473790-45473809 None:intergenic 45.0%
!! TCTTGGTGTCGTCAATCACT+TGG - chr2.4:45472729-45472748 None:intergenic 45.0%
AACACTGAGTCCAATCTGGC+AGG - chr2.4:45472875-45472894 None:intergenic 50.0%
AAGGTAGCCTTTGACAGAGG+TGG + chr2.4:45473755-45473774 MS.gene85312:CDS 50.0%
AGAGGTGGTTACCCATACCA+TGG + chr2.4:45473770-45473789 MS.gene85312:CDS 50.0%
CAGGAAGTAGCAAAGAGGGT+GGG + chr2.4:45473692-45473711 MS.gene85312:intron 50.0%
GATCACAGGGATGATCACGA+GGG + chr2.4:45473009-45473028 MS.gene85312:intron 50.0%
GCTTTAACACGGCCATGGTA+TGG - chr2.4:45473785-45473804 None:intergenic 50.0%
TCAGGAAGTAGCAAAGAGGG+TGG + chr2.4:45473691-45473710 MS.gene85312:intron 50.0%
TGATCACAGGGATGATCACG+AGG + chr2.4:45473008-45473027 MS.gene85312:intron 50.0%
TGGGTAACCACCTCTGTCAA+AGG - chr2.4:45473765-45473784 None:intergenic 50.0%
GCACAGTGGCTACCATATCG+TGG + chr2.4:45473370-45473389 MS.gene85312:intron 55.0%
GCTGATGCAGCTCGCGAACA+TGG + chr2.4:45473809-45473828 MS.gene85312:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 45472652 45473844 45472652 ID=MS.gene85312
chr2.4 mRNA 45472652 45473844 45472652 ID=MS.gene85312.t1;Parent=MS.gene85312
chr2.4 exon 45472652 45472825 45472652 ID=MS.gene85312.t1.exon1;Parent=MS.gene85312.t1
chr2.4 CDS 45472652 45472825 45472652 ID=cds.MS.gene85312.t1;Parent=MS.gene85312.t1
chr2.4 exon 45473695 45473844 45473695 ID=MS.gene85312.t1.exon2;Parent=MS.gene85312.t1
chr2.4 CDS 45473695 45473844 45473695 ID=cds.MS.gene85312.t1;Parent=MS.gene85312.t1
Gene Sequence

>MS.gene85312

ATGATTTTTCAGGTTGAAGGAACACCCGAAAGACCAAGATTGTCTGTGTTTCGATCCAACAAGCATCTCTTTGTCCAAGTGATTGACGACACCAAGATGCATACACTTGCTTCAGCTTCAACAATGCAGAAGGCAATTGCTGAAGAGTTGAACTTCACCTCTGGTCCTACAATTGTAAGTTCCTGATACGCTTTTACGTTTCTCTGATTATAATTTAACTCCTGCCAGATTGGACTCAGTGTTGTGTATATTTCTGCTATTCTACCCCTATTCCGTGATGATAATAAAAATTCCTTTTTTCCTAAATACTTATTAAAAGTCATAATGATTCCATAACAATGCATAAAACTAAACACTGATCACAGGGATGATCACGAGGGATGCACCAGATTTCAAAGAAAGATGTTGACCTGCATATTATTACTACTTCATTCTTATTATCATTTTCTCTTCTCTCTCTATGAGTAAATTCTTTACCTATTAGCCAAAAGGTTTATTCTTGGATAGAATTTTTAAACTTGACATAGAAGTTAAACCATGGAGAAGGAGCAACCTTTTAGTTTCCTTGAGTATATTACAGAAGCATGACACCCTTTGCATCCTGAAGTTCTTTTCCATTTCCAATGGTAATAACTAATATATTACTTTAGTGTTTCATTTTCATTGATCACAAGGGTCCCCATAATAGCTACTGAACATATGAGGCAATGACTTGAAAGCACAGTGGCTACCATATCGTGGATTTCTACGACAGGGTTCCTTAAAAAAAAAGTCCTGGAATCTACCAATAAGTTCAACCAAGCAAATGGTCTTCAACAACTATTCGATATTTGTATTAGAACAATTGTATATTCATTTCAGACTTCCCTTTCATTATTAAATCGTAGAAAGTTCTAATTGAGCATAGGTTTATAAAGACATATCTCAGTATAGTTGATATCCCTCAATCTCCCTTGCTTAAAAGCTTGTTGTATCTGCTATTCATTTTTAAATTTTCGTTTACCGAATGACGGTCCTGATCCTTTCAAACTGTATTGCTTCAGGAAGTAGCAAAGAGGGTGGGTGAAATCATTGCAAAATCCTGTCTGGAAATAGGGATCACAAAGGTAGCCTTTGACAGAGGTGGTTACCCATACCATGGCCGTGTTAAAGCTATTGCTGATGCAGCTCGCGAACATGGCCTCGAGTTCTAA

Protein sequence

>MS.gene85312.t1

MIFQVEGTPERPRLSVFRSNKHLFVQVIDDTKMHTLASASTMQKAIAEELNFTSGPTIEVAKRVGEIIAKSCLEIGITKVAFDRGGYPYHGRVKAIADAAREHGLEF