Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87924.t1 | XP_013451547.1 | 95.2 | 63 | 3 | 0 | 1 | 63 | 1 | 63 | 3.50E-10 | 74.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87924.t1 | I3SGW9 | 95.2 | 63 | 3 | 0 | 1 | 63 | 1 | 63 | 2.5e-10 | 74.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene87924 | 0.810389 | 1.18E-50 | -1.69E-46 |
| MS.gene049786 | MS.gene87924 | 0.810302 | 1.23E-50 | -1.69E-46 |
| MS.gene050085 | MS.gene87924 | 0.823915 | 1.07E-53 | -1.69E-46 |
| MS.gene050288 | MS.gene87924 | 0.802802 | 4.72E-49 | -1.69E-46 |
| MS.gene050317 | MS.gene87924 | 0.848751 | 5.02E-60 | -1.69E-46 |
| MS.gene050386 | MS.gene87924 | 0.817361 | 3.43E-52 | -1.69E-46 |
| MS.gene050646 | MS.gene87924 | 0.807059 | 6.08E-50 | -1.69E-46 |
| MS.gene050654 | MS.gene87924 | 0.827608 | 1.42E-54 | -1.69E-46 |
| MS.gene050752 | MS.gene87924 | 0.807127 | 5.88E-50 | -1.69E-46 |
| MS.gene050838 | MS.gene87924 | 0.828623 | 8.11E-55 | -1.69E-46 |
| MS.gene051003 | MS.gene87924 | 0.826283 | 2.95E-54 | -1.69E-46 |
| MS.gene051245 | MS.gene87924 | 0.82004 | 8.46E-53 | -1.69E-46 |
| MS.gene051343 | MS.gene87924 | 0.851253 | 1.00E-60 | -1.69E-46 |
| MS.gene051676 | MS.gene87924 | 0.835251 | 1.87E-56 | -1.69E-46 |
| MS.gene051902 | MS.gene87924 | 0.831517 | 1.60E-55 | -1.69E-46 |
| MS.gene051975 | MS.gene87924 | 0.847867 | 8.81E-60 | -1.69E-46 |
| MS.gene052072 | MS.gene87924 | 0.815736 | 7.94E-52 | -1.69E-46 |
| MS.gene052242 | MS.gene87924 | -0.816158 | 6.39E-52 | -1.69E-46 |
| MS.gene052434 | MS.gene87924 | 0.836531 | 8.86E-57 | -1.69E-46 |
| MS.gene053058 | MS.gene87924 | 0.831182 | 1.93E-55 | -1.69E-46 |
| MS.gene053167 | MS.gene87924 | 0.808061 | 3.73E-50 | -1.69E-46 |
| MS.gene053381 | MS.gene87924 | 0.815739 | 7.92E-52 | -1.69E-46 |
| MS.gene053564 | MS.gene87924 | 0.844289 | 8.29E-59 | -1.69E-46 |
| MS.gene054403 | MS.gene87924 | 0.802548 | 5.33E-49 | -1.69E-46 |
| MS.gene054536 | MS.gene87924 | 0.847372 | 1.21E-59 | -1.69E-46 |
| MS.gene05469 | MS.gene87924 | 0.813619 | 2.33E-51 | -1.69E-46 |
| MS.gene055311 | MS.gene87924 | 0.855437 | 6.31E-62 | -1.69E-46 |
| MS.gene055313 | MS.gene87924 | 0.828342 | 9.48E-55 | -1.69E-46 |
| MS.gene055314 | MS.gene87924 | 0.838814 | 2.30E-57 | -1.69E-46 |
| MS.gene055513 | MS.gene87924 | 0.801864 | 7.36E-49 | -1.69E-46 |
| MS.gene055603 | MS.gene87924 | 0.808155 | 3.56E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene87924 | 0.849102 | 4.01E-60 | -1.69E-46 |
| MS.gene056580 | MS.gene87924 | 0.820693 | 5.99E-53 | -1.69E-46 |
| MS.gene056752 | MS.gene87924 | 0.826701 | 2.35E-54 | -1.69E-46 |
| MS.gene056787 | MS.gene87924 | 0.861898 | 7.42E-64 | -1.69E-46 |
| MS.gene056849 | MS.gene87924 | 0.832749 | 7.91E-56 | -1.69E-46 |
| MS.gene056986 | MS.gene87924 | 0.825319 | 5.00E-54 | -1.69E-46 |
| MS.gene057094 | MS.gene87924 | 0.806468 | 8.11E-50 | -1.69E-46 |
| MS.gene057234 | MS.gene87924 | 0.83482 | 2.40E-56 | -1.69E-46 |
| MS.gene057452 | MS.gene87924 | -0.817322 | 3.50E-52 | -1.69E-46 |
| MS.gene05781 | MS.gene87924 | 0.807939 | 3.96E-50 | -1.69E-46 |
| MS.gene057890 | MS.gene87924 | -0.834592 | 2.74E-56 | -1.69E-46 |
| MS.gene057999 | MS.gene87924 | 0.846064 | 2.75E-59 | -1.69E-46 |
| MS.gene058054 | MS.gene87924 | 0.827636 | 1.40E-54 | -1.69E-46 |
| MS.gene058055 | MS.gene87924 | 0.823857 | 1.10E-53 | -1.69E-46 |
| MS.gene058074 | MS.gene87924 | 0.81649 | 5.38E-52 | -1.69E-46 |
| MS.gene058238 | MS.gene87924 | 0.805372 | 1.38E-49 | -1.69E-46 |
| MS.gene058639 | MS.gene87924 | 0.812399 | 4.32E-51 | -1.69E-46 |
| MS.gene058646 | MS.gene87924 | 0.806778 | 6.97E-50 | -1.69E-46 |
| MS.gene058654 | MS.gene87924 | 0.819991 | 8.68E-53 | -1.69E-46 |
| MS.gene059050 | MS.gene87924 | 0.814229 | 1.71E-51 | -1.69E-46 |
| MS.gene059146 | MS.gene87924 | 0.827135 | 1.85E-54 | -1.69E-46 |
| MS.gene059171 | MS.gene87924 | 0.807805 | 4.23E-50 | -1.69E-46 |
| MS.gene059360 | MS.gene87924 | 0.812228 | 4.71E-51 | -1.69E-46 |
| MS.gene06010 | MS.gene87924 | 0.811323 | 7.42E-51 | -1.69E-46 |
| MS.gene06011 | MS.gene87924 | 0.841698 | 4.06E-58 | -1.69E-46 |
| MS.gene060259 | MS.gene87924 | 0.803443 | 3.48E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene87924 | 0.811005 | 8.69E-51 | -1.69E-46 |
| MS.gene060869 | MS.gene87924 | 0.820221 | 7.69E-53 | -1.69E-46 |
| MS.gene060870 | MS.gene87924 | 0.815243 | 1.02E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87924.t1 | MTR_6g027810 | 96.000 | 100 | 2 | 1 | 1 | 98 | 1 | 100 | 3.22e-52 | 159 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene87924.t1 | AT1G54410 | 56.757 | 74 | 23 | 2 | 1 | 73 | 1 | 66 | 5.47e-12 | 57.4 |
Find 20 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACCTTTGTGCTTATCATCA+TGG | 0.347190 | 6.1:-10393981 | None:intergenic |
| AAAGAAGGATAAGAAGAAGA+AGG | 0.460441 | 6.1:+10394092 | MS.gene87924:CDS |
| GCAGGAATCATCAACAAGAT+TGG | 0.467463 | 6.1:+10393868 | MS.gene87924:CDS |
| GACAAGATCCATGGTGGTGA+AGG | 0.514151 | 6.1:+10394036 | MS.gene87924:CDS |
| AGGTATAGCTGAGAAGATCA+AGG | 0.562746 | 6.1:+10394014 | MS.gene87924:CDS |
| AGTCACAAACAAGTCATGGC+AGG | 0.585630 | 6.1:+10393850 | None:intergenic |
| ATTGGAGAGGCTCTTCATGT+AGG | 0.594022 | 6.1:+10393886 | MS.gene87924:CDS |
| GAGGCTCTTCATGTAGGAGG+AGG | 0.594348 | 6.1:+10393892 | MS.gene87924:CDS |
| GGAGAGGCTCTTCATGTAGG+AGG | 0.607476 | 6.1:+10393889 | MS.gene87924:CDS |
| AAGATCAAGGACAAGATCCA+TGG | 0.615180 | 6.1:+10394027 | MS.gene87924:CDS |
| CACAAAGGTGAACACAAGGA+AGG | 0.629648 | 6.1:+10393994 | MS.gene87924:CDS |
| GTTCATGACCTTCACCACCA+TGG | 0.630380 | 6.1:-10394044 | None:intergenic |
| GATAAGAAGAAGAAGGAACA+TGG | 0.650367 | 6.1:+10394099 | MS.gene87924:CDS |
| AAGAAAGAAGATGAACACAA+AGG | 0.654433 | 6.1:+10393919 | MS.gene87924:CDS |
| TAAGCACAAAGGTGAACACA+AGG | 0.681644 | 6.1:+10393990 | MS.gene87924:CDS |
| AATCATCAACAAGATTGGAG+AGG | 0.688291 | 6.1:+10393873 | MS.gene87924:CDS |
| AGCCATGATGATAAGCACAA+AGG | 0.698703 | 6.1:+10393979 | MS.gene87924:CDS |
| AGTCATGATGACAAGCACAA+AGG | 0.702474 | 6.1:+10393949 | MS.gene87924:CDS |
| ATCAAGGACAAGATCCATGG+TGG | 0.715898 | 6.1:+10394030 | MS.gene87924:CDS |
| GGTGAAGGTCATGAACACAA+AGG | 0.801234 | 6.1:+10394051 | MS.gene87924:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAGAAAGATAAGAAAAAGA+AGG | + | chr6.1:10394077-10394096 | MS.gene87924:CDS | 25.0% |
| AAAGAAGGATAAGAAGAAGA+AGG | + | chr6.1:10394092-10394111 | MS.gene87924:CDS | 30.0% | |
| AAGAAAGAAGATGAACACAA+AGG | + | chr6.1:10393919-10393938 | MS.gene87924:CDS | 30.0% | |
| AATCATCAACAAGATTGGAG+AGG | + | chr6.1:10393873-10393892 | MS.gene87924:CDS | 35.0% | |
| GATAAGAAGAAGAAGGAACA+TGG | + | chr6.1:10394099-10394118 | MS.gene87924:CDS | 35.0% | |
| AAGATCAAGGACAAGATCCA+TGG | + | chr6.1:10394027-10394046 | MS.gene87924:CDS | 40.0% | |
| AGCCATGATGATAAGCACAA+AGG | + | chr6.1:10393979-10393998 | MS.gene87924:CDS | 40.0% | |
| AGGTATAGCTGAGAAGATCA+AGG | + | chr6.1:10394014-10394033 | MS.gene87924:CDS | 40.0% | |
| AGTCATGATGACAAGCACAA+AGG | + | chr6.1:10393949-10393968 | MS.gene87924:CDS | 40.0% | |
| CACCTTTGTGCTTATCATCA+TGG | - | chr6.1:10393984-10394003 | None:intergenic | 40.0% | |
| GCAGGAATCATCAACAAGAT+TGG | + | chr6.1:10393868-10393887 | MS.gene87924:CDS | 40.0% | |
| TAAGCACAAAGGTGAACACA+AGG | + | chr6.1:10393990-10394009 | MS.gene87924:CDS | 40.0% | |
| ATCAAGGACAAGATCCATGG+TGG | + | chr6.1:10394030-10394049 | MS.gene87924:CDS | 45.0% | |
| ATTGGAGAGGCTCTTCATGT+AGG | + | chr6.1:10393886-10393905 | MS.gene87924:CDS | 45.0% | |
| CACAAAGGTGAACACAAGGA+AGG | + | chr6.1:10393994-10394013 | MS.gene87924:CDS | 45.0% | |
| GGTGAAGGTCATGAACACAA+AGG | + | chr6.1:10394051-10394070 | MS.gene87924:CDS | 45.0% | |
| GTTCATGACCTTCACCACCA+TGG | - | chr6.1:10394047-10394066 | None:intergenic | 50.0% | |
| ! | GACAAGATCCATGGTGGTGA+AGG | + | chr6.1:10394036-10394055 | MS.gene87924:CDS | 50.0% |
| GAGGCTCTTCATGTAGGAGG+AGG | + | chr6.1:10393892-10393911 | MS.gene87924:CDS | 55.0% | |
| GGAGAGGCTCTTCATGTAGG+AGG | + | chr6.1:10393889-10393908 | MS.gene87924:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 10393865 | 10394161 | 10393865 | ID=MS.gene87924 |
| chr6.1 | mRNA | 10393865 | 10394161 | 10393865 | ID=MS.gene87924.t1;Parent=MS.gene87924 |
| chr6.1 | exon | 10393865 | 10394161 | 10393865 | ID=MS.gene87924.t1.exon1;Parent=MS.gene87924.t1 |
| chr6.1 | CDS | 10393865 | 10394161 | 10393865 | ID=cds.MS.gene87924.t1;Parent=MS.gene87924.t1 |
| Gene Sequence |
| Protein sequence |
