AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92590

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92590.t1 MTR_4g019310 95.954 346 13 1 1 345 155 500 0.0 672
MS.gene92590.t1 MTR_4g017470 41.127 355 181 10 13 344 129 478 5.32e-69 224
MS.gene92590.t1 MTR_4g017830 39.227 362 180 10 8 342 159 507 2.25e-66 218
MS.gene92590.t1 MTR_4g017830 26.693 251 159 8 96 333 1 239 1.05e-13 72.4
MS.gene92590.t1 MTR_4g017550 41.667 348 171 11 6 332 133 469 6.62e-65 213
MS.gene92590.t1 MTR_4g019340 40.483 331 143 9 16 323 202 501 4.24e-62 206
MS.gene92590.t1 MTR_4g019340 27.863 262 161 10 95 333 19 275 1.06e-13 72.4
MS.gene92590.t1 MTR_4g017440 39.458 332 186 8 21 340 178 506 1.52e-60 202
MS.gene92590.t1 MTR_4g017410 37.912 364 173 10 4 345 161 493 1.33e-59 200
MS.gene92590.t1 MTR_4g017410 25.306 245 159 9 108 333 5 244 9.27e-11 63.2
MS.gene92590.t1 MTR_4g017610 38.769 325 173 8 16 332 169 475 1.19e-54 186
MS.gene92590.t1 MTR_7g066570 32.955 352 196 11 12 345 160 489 1.06e-45 164
MS.gene92590.t1 MTR_7g066570 30.204 245 142 11 108 342 10 235 4.18e-15 76.6
MS.gene92590.t1 MTR_7g066350 31.988 322 163 7 12 319 154 433 6.20e-45 160
MS.gene92590.t1 MTR_7g066350 31.780 236 141 7 108 333 5 230 2.98e-17 82.8
MS.gene92590.t1 MTR_7g066260 35.802 324 164 8 19 332 169 458 4.79e-44 158
MS.gene92590.t1 MTR_7g066260 29.289 239 145 13 108 333 11 238 7.43e-13 69.7
MS.gene92590.t1 MTR_4g019330 43.578 218 61 5 126 342 32 188 8.54e-44 149
MS.gene92590.t1 MTR_7g063920 34.234 333 201 8 13 342 119 436 7.22e-43 154
MS.gene92590.t1 MTR_7g058710 31.232 349 211 9 10 342 148 483 7.84e-43 155
MS.gene92590.t1 MTR_7g058710 28.761 226 143 9 113 333 13 225 2.48e-15 77.0
MS.gene92590.t1 MTR_7g062280 33.234 334 198 7 19 342 173 491 2.17e-42 154
MS.gene92590.t1 MTR_7g066340 33.538 325 166 7 19 332 164 449 1.13e-41 151
MS.gene92590.t1 MTR_7g066340 29.362 235 150 6 108 333 8 235 2.56e-17 83.2
MS.gene92590.t1 MTR_7g066430 34.320 338 202 9 15 337 154 486 2.53e-41 151
MS.gene92590.t1 MTR_7g066400 32.938 337 173 9 16 332 130 433 2.76e-41 150
MS.gene92590.t1 MTR_7g063660 34.421 337 193 11 19 342 156 477 4.58e-41 150
MS.gene92590.t1 MTR_3g007740 33.634 333 171 10 19 336 145 442 2.21e-39 144
MS.gene92590.t1 MTR_7g066580 32.313 294 172 8 49 324 150 434 3.47e-39 144
MS.gene92590.t1 MTR_1g041315 30.960 323 163 7 19 319 167 451 4.48e-38 142
MS.gene92590.t1 MTR_7g062300 31.889 323 201 8 19 335 163 472 9.05e-38 140
MS.gene92590.t1 MTR_7g063890 33.333 333 205 8 19 340 176 502 1.01e-37 141
MS.gene92590.t1 MTR_7g063890 26.809 235 147 11 115 339 30 249 3.80e-11 64.3
MS.gene92590.t1 MTR_7g063670 33.939 330 183 11 19 342 157 457 1.09e-37 140
MS.gene92590.t1 MTR_7g063880 30.769 338 205 10 13 343 154 469 1.58e-36 137
MS.gene92590.t1 MTR_7g066410 31.389 360 204 11 13 336 160 512 1.80e-35 135
MS.gene92590.t1 MTR_7g063900 34.132 334 198 11 19 338 159 484 2.07e-35 134
MS.gene92590.t1 MTR_7g066680 31.579 266 149 6 68 315 37 287 6.94e-35 130
MS.gene92590.t1 MTR_7g063840 30.448 335 194 10 24 342 162 473 8.03e-35 133
MS.gene92590.t1 MTR_7g012680 33.234 337 169 12 13 340 160 449 4.07e-34 130
MS.gene92590.t1 MTR_7g012680 27.805 205 111 8 108 294 10 195 1.92e-12 68.2
MS.gene92590.t1 MTR_7g066360 34.146 328 160 10 16 332 163 445 3.36e-33 128
MS.gene92590.t1 MTR_7g063820 30.199 351 230 8 3 342 138 484 5.85e-33 128
MS.gene92590.t1 MTR_7g066280 30.861 337 187 11 19 332 73 386 5.57e-32 124
MS.gene92590.t1 MTR_7g066290 32.087 321 151 8 19 332 169 429 1.80e-30 120
MS.gene92590.t1 MTR_3g065200 28.664 307 176 7 19 322 142 408 5.02e-29 116
MS.gene92590.t1 MTR_7g066310 34.199 231 114 6 104 332 185 379 1.19e-28 115
MS.gene92590.t1 MTR_2g029400 29.206 315 199 8 13 324 111 404 3.93e-27 110
MS.gene92590.t1 MTR_7g063720 30.638 235 138 5 109 341 20 231 2.63e-26 105
MS.gene92590.t1 MTR_7g063830 30.952 336 180 13 13 342 155 444 7.06e-26 107
MS.gene92590.t1 MTR_7g066480 31.481 324 145 9 19 332 161 417 1.62e-25 106
MS.gene92590.t1 MTR_7g058700 35.749 207 100 6 10 200 653 842 4.32e-24 103
MS.gene92590.t1 MTR_7g100420 33.210 271 166 9 68 333 134 394 7.06e-22 95.9
MS.gene92590.t1 MTR_7g063850 29.060 234 148 9 115 343 69 289 6.39e-21 91.7
MS.gene92590.t1 MTR_1g014060 33.333 210 106 9 131 332 197 380 6.11e-20 90.1
MS.gene92590.t1 MTR_7g066490 37.791 172 83 5 19 180 160 317 6.86e-20 90.5
MS.gene92590.t1 MTR_7g066490 56.522 69 29 1 264 332 336 403 5.67e-14 72.8
MS.gene92590.t1 MTR_1g115440 27.313 227 149 5 113 329 12 232 3.79e-18 82.8
MS.gene92590.t1 MTR_7g066390 38.938 113 58 4 224 332 13 118 5.33e-14 69.3
MS.gene92590.t1 MTR_7g063690 34.959 123 78 2 19 139 100 222 5.50e-14 71.2
MS.gene92590.t1 MTR_8g023240 22.601 323 220 8 16 323 170 477 8.22e-14 72.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92590.t1 AT5G01140 33.526 346 202 9 7 342 131 458 4.86e-41 150
MS.gene92590.t1 AT3G09140 33.533 334 197 11 15 336 173 493 2.43e-35 135
MS.gene92590.t1 AT5G01130 31.098 328 181 7 25 343 161 452 3.22e-35 134
MS.gene92590.t1 AT5G01120 31.515 330 208 7 18 335 165 488 6.48e-35 134
MS.gene92590.t1 AT5G01150 31.176 340 212 7 15 343 159 487 2.86e-34 132
MS.gene92590.t1 AT3G09140 30.939 362 197 12 15 336 173 521 7.78e-30 119
MS.gene92590.t1 AT5G43240 31.638 354 205 12 4 335 10 348 1.22e-25 106
MS.gene92590.t1 AT5G43240 31.549 355 204 12 4 335 154 492 7.04e-25 105
MS.gene92590.t1 AT5G43240 31.549 355 204 12 4 335 154 492 7.04e-25 105
MS.gene92590.t1 AT5G43240 31.549 355 204 12 4 335 154 492 7.04e-25 105
MS.gene92590.t1 AT3G09110 39.264 163 95 1 16 174 157 319 5.37e-24 101
MS.gene92590.t1 AT3G09120 39.873 158 90 2 13 166 157 313 6.16e-24 100
MS.gene92590.t1 AT3G09120 30.213 235 141 10 109 329 3 228 3.27e-13 70.1
MS.gene92590.t1 AT5G37320 29.619 341 202 13 15 341 168 484 1.54e-19 89.7

Find 63 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAAGGTGGCTCCTCAATT+TGG 0.239522 4.2:+82961066 MS.gene92590:CDS
ATGAAGTTGCATGGAGATTT+TGG 0.248004 4.2:+82959553 MS.gene92590:CDS
GCCTTCTGTCAAAGCCTCTT+TGG 0.291747 4.2:-82961797 None:intergenic
TCATGGTTCATAAGGCCTTT+AGG 0.297689 4.2:+82961027 MS.gene92590:CDS
CATCTTCCTTTGGATTCTTA+AGG 0.307194 4.2:-82961831 None:intergenic
TGGCACAATTGAATCTGCTT+TGG 0.331646 4.2:+82961266 MS.gene92590:CDS
TGAGGAGCCACCTTTGGATT+TGG 0.337613 4.2:-82961060 None:intergenic
ACACCAATTGGATCTATTCT+AGG 0.341690 4.2:+82960934 MS.gene92590:CDS
GGCACAATTGAATCTGCTTT+GGG 0.350892 4.2:+82961267 MS.gene92590:CDS
GAATGTACCATTGGATGATT+TGG 0.353597 4.2:+82961383 MS.gene92590:CDS
CTCTTTCAATCCAACATTCA+AGG 0.359070 4.2:-82959734 None:intergenic
CCAAATTGAGGAGCCACCTT+TGG 0.362907 4.2:-82961066 None:intergenic
ATTGTGCCATAAGGTGATCT+TGG 0.386765 4.2:-82961252 None:intergenic
TTTGATCCAAGATCACCTTA+TGG 0.391081 4.2:+82961246 MS.gene92590:CDS
TCAATCCTTCATGGTTCATA+AGG 0.394940 4.2:+82961019 MS.gene92590:CDS
ATTTGTTATTCCAATGTGAA+TGG 0.400477 4.2:+82961171 MS.gene92590:CDS
CAAGCTGTAACATCTTCCTT+TGG 0.403117 4.2:-82961841 None:intergenic
AGTTTCCTCACAACACCAAT+TGG 0.406778 4.2:+82960922 MS.gene92590:CDS
TGAAAGAATTCAATCCTTCA+TGG 0.410331 4.2:+82961010 MS.gene92590:CDS
ATGGACTAATATAAAACTTA+AGG 0.429845 4.2:+82960828 MS.gene92590:CDS
TCTTCACAAAGATTTATTAG+TGG 0.444473 4.2:-82961102 None:intergenic
AGCCACCTTTGGATTTGGTA+AGG 0.450367 4.2:-82961055 None:intergenic
TGTCAGGCACTGAACTTGTT+GGG 0.468783 4.2:+82961757 MS.gene92590:intron
TTGTCAGGCACTGAACTTGT+TGG 0.475529 4.2:+82961756 MS.gene92590:intron
TCCAAAGAGGCTTTGACAGA+AGG 0.476288 4.2:+82961796 MS.gene92590:CDS
TAATATAAAACTTAAGGTAA+TGG 0.478261 4.2:+82960834 MS.gene92590:CDS
TTCTTTGTCTTCTTCCAAAG+AGG 0.481827 4.2:+82961783 MS.gene92590:CDS
CCACCTTTGGATTTGGTAAG+GGG 0.490596 4.2:-82961053 None:intergenic
CCTACTTATTGGTATGGTAA+TGG 0.492706 4.2:+82961132 MS.gene92590:CDS
GTTCAGCAACTCATTCAACT+TGG 0.493028 4.2:+82959679 MS.gene92590:CDS
GCAGATTCAATTGTGCCATA+AGG 0.493493 4.2:-82961261 None:intergenic
TATATCAGTGAGAGGAGACT+TGG 0.494239 4.2:-82960737 None:intergenic
CTGCTTTGGGATTTGTGAAG+AGG 0.503837 4.2:+82961280 MS.gene92590:CDS
AAAGTGTTGCAAAACTCTCC+TGG 0.503982 4.2:+82959649 MS.gene92590:CDS
TGCTTCATGCAACCAAATTG+AGG 0.504685 4.2:-82961078 None:intergenic
TTTGGTAAGGGGTTACCTAA+AGG 0.505371 4.2:-82961042 None:intergenic
GACAAAGAAATGAAGTTGCA+TGG 0.513467 4.2:+82959544 None:intergenic
GCATAAGATGAATGTACCAT+TGG 0.517517 4.2:+82961374 MS.gene92590:CDS
GAGGAGCATATAGTGAGCAT+TGG 0.518756 4.2:+82961405 MS.gene92590:CDS
GCCACCTTTGGATTTGGTAA+GGG 0.525081 4.2:-82961054 None:intergenic
ACACAAGTGTCCTTGAATGT+TGG 0.529675 4.2:+82959724 MS.gene92590:CDS
ATGCTTGAAGTATTTGCAAA+TGG 0.530077 4.2:-82961342 None:intergenic
CCATTACCATACCAATAAGT+AGG 0.533560 4.2:-82961132 None:intergenic
GGCCATCACTATTTATTGTG+TGG 0.534751 4.2:+82961301 MS.gene92590:CDS
GCCATCACTATTTATTGTGT+GGG 0.535594 4.2:+82961302 MS.gene92590:CDS
AAAGCTCCTACTTATTGGTA+TGG 0.537812 4.2:+82961126 MS.gene92590:CDS
TGTCCTAGAATAGATCCAAT+TGG 0.538385 4.2:-82960937 None:intergenic
CTTGAATGTTGGATTGAAAG+AGG 0.547131 4.2:+82959735 MS.gene92590:CDS
CTTCCTTTGGATTCTTAAGG+AGG 0.549103 4.2:-82961828 None:intergenic
TCAACATTCTCGCCAAGACA+TGG 0.561002 4.2:+82960790 MS.gene92590:CDS
TGATATCACTCCATTCACAT+TGG 0.568520 4.2:-82961181 None:intergenic
TGTACCATTGGATGATTTGG+AGG 0.570776 4.2:+82961386 MS.gene92590:CDS
GATACTATATGCTGAAGCTG+AGG 0.584783 4.2:+82960876 MS.gene92590:CDS
AAAGGCCTTATGAACCATGA+AGG 0.592293 4.2:-82961024 None:intergenic
TCCCACACAATAAATAGTGA+TGG 0.607267 4.2:-82961303 None:intergenic
TGCTCCTCCAAATCATCCAA+TGG 0.607505 4.2:-82961390 None:intergenic
ATACTATATGCTGAAGCTGA+GGG 0.609419 4.2:+82960877 MS.gene92590:CDS
CTACCTCCTTAAGAATCCAA+AGG 0.611859 4.2:+82961825 MS.gene92590:CDS
AGTTGCTGAACTGAATTACC+AGG 0.611897 4.2:-82959667 None:intergenic
TAGATCCAATTGGTGTTGTG+AGG 0.624363 4.2:-82960927 None:intergenic
CCCCTTACCAAATCCAAAGG+TGG 0.639710 4.2:+82961053 MS.gene92590:CDS
TAACCCCTTACCAAATCCAA+AGG 0.647813 4.2:+82961050 MS.gene92590:CDS
AGTGAGCATTGGAGAAACAG+AGG 0.672512 4.2:+82961416 MS.gene92590:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATTTTTAATATATTTTT+AGG + chr4.2:82959772-82959791 MS.gene92590:intron 0.0%
!! AAAAAATAATAATTTAGACA+TGG + chr4.2:82960517-82960536 MS.gene92590:intron 10.0%
!! AAAATTAATTAAAGAACTTA+AGG - chr4.2:82960589-82960608 None:intergenic 10.0%
!! TATTTATATGGTAATTAAAT+TGG + chr4.2:82960034-82960053 MS.gene92590:intron 10.0%
!!! ATTTTCTTGTTAAATTTTTT+TGG - chr4.2:82961585-82961604 None:intergenic 10.0%
!! AAAATTCTAATTTAAATGTG+AGG + chr4.2:82960234-82960253 MS.gene92590:intron 15.0%
!! TAATATAAAACTTAAGGTAA+TGG + chr4.2:82960834-82960853 MS.gene92590:CDS 15.0%
!!! AATTTTTCATGGTATTTATA+TGG + chr4.2:82960022-82960041 MS.gene92590:intron 15.0%
!!! TAATTTTTAAACATGTGTAT+GGG + chr4.2:82960602-82960621 MS.gene92590:intron 15.0%
!!! TATATTTTTAGGAAAATGTT+TGG + chr4.2:82959783-82959802 MS.gene92590:intron 15.0%
!!! TTAATTTTTAAACATGTGTA+TGG + chr4.2:82960601-82960620 MS.gene92590:intron 15.0%
!! GGTTTACTCATTATAAAATT+AGG + chr4.2:82961671-82961690 MS.gene92590:intron 20.0%
!! TAACAAGAAAATAGATGTTA+AGG + chr4.2:82961593-82961612 MS.gene92590:intron 20.0%
!! TTAATTAATTTATCTTGTCC+TGG + chr4.2:82961455-82961474 MS.gene92590:intron 20.0%
!! TTAATTATCTCTATATTGTC+AGG + chr4.2:82961741-82961760 MS.gene92590:intron 20.0%
!! TTACTCATTATAAAATTAGG+TGG + chr4.2:82961674-82961693 MS.gene92590:intron 20.0%
!!! AAAATCTGGAATGTTAAAAT+AGG - chr4.2:82960691-82960710 None:intergenic 20.0%
!!! ATGGACTAATATAAAACTTA+AGG + chr4.2:82960828-82960847 MS.gene92590:CDS 20.0%
!!! CATCATTTTAAATTCTTCAT+TGG - chr4.2:82960770-82960789 None:intergenic 20.0%
!!! CTGATATTTTGTAAATTAAC+TGG - chr4.2:82959973-82959992 None:intergenic 20.0%
!!! TATTTTAACATTCCAGATTT+TGG + chr4.2:82960690-82960709 MS.gene92590:intron 20.0%
!!! TTAGTCCATTTTTCAAATTT+TGG - chr4.2:82960817-82960836 None:intergenic 20.0%
! AAAATTGACCCTAAATACAT+AGG - chr4.2:82960008-82960027 None:intergenic 25.0%
! ACAAATTAGACAAAATAGAG+AGG + chr4.2:82960325-82960344 MS.gene92590:intron 25.0%
! ATGGACCAAAATTTGAAAAA+TGG + chr4.2:82960809-82960828 MS.gene92590:CDS 25.0%
! ATGTAGAAAAGTGAAGAATT+CGG - chr4.2:82960428-82960447 None:intergenic 25.0%
! CAAATTAGACAAAATAGAGA+GGG + chr4.2:82960326-82960345 MS.gene92590:intron 25.0%
! TCTTCACAAAGATTTATTAG+TGG - chr4.2:82961105-82961124 None:intergenic 25.0%
!! AAAACTGACTTTAAAAATCG+TGG + chr4.2:82960274-82960293 MS.gene92590:intron 25.0%
!! TTGAATGACAATTTTTCTCT+AGG + chr4.2:82960961-82960980 MS.gene92590:CDS 25.0%
!!! ATTTGTTATTCCAATGTGAA+TGG + chr4.2:82961171-82961190 MS.gene92590:CDS 25.0%
!!! ATTTTTTTTACCTCCTTAAC+CGG + chr4.2:82961533-82961552 MS.gene92590:intron 25.0%
!!! GAGAGTTATAATTTTGATGA+TGG + chr4.2:82959580-82959599 MS.gene92590:CDS 25.0%
!!! GTTTGGCTTATATATAGTTT+TGG + chr4.2:82959889-82959908 MS.gene92590:intron 25.0%
!!! TATTTAGGGTCAATTTTTCA+TGG + chr4.2:82960011-82960030 MS.gene92590:intron 25.0%
!!! TTTTGTCTAATTTGTGACAT+TGG - chr4.2:82960320-82960339 None:intergenic 25.0%
AAGAAAAAGCTCCTACTTAT+TGG + chr4.2:82961121-82961140 MS.gene92590:CDS 30.0%
ATCAATTGAGCTACACTTAT+GGG - chr4.2:82960364-82960383 None:intergenic 30.0%
CACGTCATCATTAATTAAGT+TGG + chr4.2:82960182-82960201 MS.gene92590:intron 30.0%
TATATATATGTGTGTGTGTG+TGG + chr4.2:82961650-82961669 MS.gene92590:intron 30.0%
TATCAATTGAGCTACACTTA+TGG - chr4.2:82960365-82960384 None:intergenic 30.0%
TCAAAAAGTTTCATTGCTTC+TGG - chr4.2:82961231-82961250 None:intergenic 30.0%
TGAAAGAATTCAATCCTTCA+TGG + chr4.2:82961010-82961029 MS.gene92590:CDS 30.0%
TGTAGAAAAGTGAAGAATTC+GGG - chr4.2:82960427-82960446 None:intergenic 30.0%
TTTCTCCACCTATGTATTTA+GGG + chr4.2:82959997-82960016 MS.gene92590:intron 30.0%
! ATGCTTGAAGTATTTGCAAA+TGG - chr4.2:82961345-82961364 None:intergenic 30.0%
! GACTTTAAAAATCGTGGATT+TGG + chr4.2:82960280-82960299 MS.gene92590:intron 30.0%
! TCCTCTCACTGATATATTTT+TGG + chr4.2:82960744-82960763 MS.gene92590:CDS 30.0%
! TTTTCTCCACCTATGTATTT+AGG + chr4.2:82959996-82960015 MS.gene92590:intron 30.0%
!! GATGGTGTTTTTGTTAAGAA+AGG + chr4.2:82959598-82959617 MS.gene92590:CDS 30.0%
!! TATACACCTCCTTTAAAACT+AGG + chr4.2:82961704-82961723 MS.gene92590:intron 30.0%
!!! AATTTTGATGATGTGACAAG+TGG - chr4.2:82960220-82960239 None:intergenic 30.0%
!!! ATTTTGATGATGTGACAAGT+GGG - chr4.2:82960219-82960238 None:intergenic 30.0%
!!! CTCTATTTTTTTCTCTCTCA+AGG - chr4.2:82959833-82959852 None:intergenic 30.0%
!!! TATTTTTTTCTCTCTCAAGG+CGG - chr4.2:82959830-82959849 None:intergenic 30.0%
!!! TTACTTGCTCCTAGTTTTAA+AGG - chr4.2:82961716-82961735 None:intergenic 30.0%
AAAGCTCCTACTTATTGGTA+TGG + chr4.2:82961126-82961145 MS.gene92590:CDS 35.0%
ACACCAATTGGATCTATTCT+AGG + chr4.2:82960934-82960953 MS.gene92590:CDS 35.0%
ACTTCTACACGTAAAATCCT+TGG - chr4.2:82960392-82960411 None:intergenic 35.0%
ATACTATATGCTGAAGCTGA+GGG + chr4.2:82960877-82960896 MS.gene92590:CDS 35.0%
ATTGACCCTAAATACATAGG+TGG - chr4.2:82960005-82960024 None:intergenic 35.0%
CATCTTCCTTTGGATTCTTA+AGG - chr4.2:82961834-82961853 None:intergenic 35.0%
CCATTACCATACCAATAAGT+AGG - chr4.2:82961135-82961154 None:intergenic 35.0%
CCTACTTATTGGTATGGTAA+TGG + chr4.2:82961132-82961151 MS.gene92590:CDS 35.0%
CTCTTTCAATCCAACATTCA+AGG - chr4.2:82959737-82959756 None:intergenic 35.0%
CTTGAATGTTGGATTGAAAG+AGG + chr4.2:82959735-82959754 MS.gene92590:CDS 35.0%
GAATGTACCATTGGATGATT+TGG + chr4.2:82961383-82961402 MS.gene92590:CDS 35.0%
GCATAAGATGAATGTACCAT+TGG + chr4.2:82961374-82961393 MS.gene92590:CDS 35.0%
GCCAAAAATATATCAGTGAG+AGG - chr4.2:82960748-82960767 None:intergenic 35.0%
GCCATCACTATTTATTGTGT+GGG + chr4.2:82961302-82961321 MS.gene92590:CDS 35.0%
GTAGAAAAGTGAAGAATTCG+GGG - chr4.2:82960426-82960445 None:intergenic 35.0%
GTGGATTTGGAAAAAGCAAA+TGG + chr4.2:82960293-82960312 MS.gene92590:intron 35.0%
TCAATCCTTCATGGTTCATA+AGG + chr4.2:82961019-82961038 MS.gene92590:CDS 35.0%
TCCCACACAATAAATAGTGA+TGG - chr4.2:82961306-82961325 None:intergenic 35.0%
TGATATCACTCCATTCACAT+TGG - chr4.2:82961184-82961203 None:intergenic 35.0%
TGTCCTAGAATAGATCCAAT+TGG - chr4.2:82960940-82960959 None:intergenic 35.0%
TTAGACAAAATAGAGAGGGA+CGG + chr4.2:82960330-82960349 MS.gene92590:intron 35.0%
TTCTTTGTCTTCTTCCAAAG+AGG + chr4.2:82961783-82961802 MS.gene92590:CDS 35.0%
TTTGATCCAAGATCACCTTA+TGG + chr4.2:82961246-82961265 MS.gene92590:CDS 35.0%
! AGCTCAATTGATAGTTACCA+AGG + chr4.2:82960372-82960391 MS.gene92590:intron 35.0%
! AGGATTTTACGTGTAGAAGT+TGG + chr4.2:82960392-82960411 MS.gene92590:intron 35.0%
! ATGAAGTTGCATGGAGATTT+TGG + chr4.2:82959553-82959572 MS.gene92590:CDS 35.0%
! GATGACGTGATATTTCTACA+CGG - chr4.2:82960171-82960190 None:intergenic 35.0%
!! TCAAATTTTGGTCCATGTCT+TGG - chr4.2:82960805-82960824 None:intergenic 35.0%
!!! CAATTTTTCTCTAGGATGTG+TGG + chr4.2:82960969-82960988 MS.gene92590:CDS 35.0%
ACACAAGTGTCCTTGAATGT+TGG + chr4.2:82959724-82959743 MS.gene92590:CDS 40.0%
AGTTGCTGAACTGAATTACC+AGG - chr4.2:82959670-82959689 None:intergenic 40.0%
AGTTTCCTCACAACACCAAT+TGG + chr4.2:82960922-82960941 MS.gene92590:CDS 40.0%
ATAGAGAGAGCGAGAATGTT+TGG + chr4.2:82959872-82959891 MS.gene92590:intron 40.0%
ATTGTGCCATAAGGTGATCT+TGG - chr4.2:82961255-82961274 None:intergenic 40.0%
CAAGCTGTAACATCTTCCTT+TGG - chr4.2:82961844-82961863 None:intergenic 40.0%
CTACCTCCTTAAGAATCCAA+AGG + chr4.2:82961825-82961844 MS.gene92590:CDS 40.0%
CTTCCTTTGGATTCTTAAGG+AGG - chr4.2:82961831-82961850 None:intergenic 40.0%
GATACTATATGCTGAAGCTG+AGG + chr4.2:82960876-82960895 MS.gene92590:CDS 40.0%
GCAGATTCAATTGTGCCATA+AGG - chr4.2:82961264-82961283 None:intergenic 40.0%
GCTTTAGCAAGTCCAAAATC+TGG - chr4.2:82960705-82960724 None:intergenic 40.0%
GGCACAATTGAATCTGCTTT+GGG + chr4.2:82961267-82961286 MS.gene92590:CDS 40.0%
GGCCATCACTATTTATTGTG+TGG + chr4.2:82961301-82961320 MS.gene92590:CDS 40.0%
GTTAAGCTAACCAGTGTCTT+GGG - chr4.2:82961563-82961582 None:intergenic 40.0%
GTTCAGCAACTCATTCAACT+TGG + chr4.2:82959679-82959698 MS.gene92590:CDS 40.0%
TAACCCCTTACCAAATCCAA+AGG + chr4.2:82961050-82961069 MS.gene92590:CDS 40.0%
TATATCAGTGAGAGGAGACT+TGG - chr4.2:82960740-82960759 None:intergenic 40.0%
TCATGGTTCATAAGGCCTTT+AGG + chr4.2:82961027-82961046 MS.gene92590:CDS 40.0%
TCCAAGTATCAAGATTTGCC+AGG - chr4.2:82961476-82961495 None:intergenic 40.0%
TCCTGGCAAATCTTGATACT+TGG + chr4.2:82961472-82961491 MS.gene92590:intron 40.0%
TGCTTCATGCAACCAAATTG+AGG - chr4.2:82961081-82961100 None:intergenic 40.0%
TGGCACAATTGAATCTGCTT+TGG + chr4.2:82961266-82961285 MS.gene92590:CDS 40.0%
TTAAGCTAACCAGTGTCTTG+GGG - chr4.2:82961562-82961581 None:intergenic 40.0%
TTTGGTAAGGGGTTACCTAA+AGG - chr4.2:82961045-82961064 None:intergenic 40.0%
! AAAGGCCTTATGAACCATGA+AGG - chr4.2:82961027-82961046 None:intergenic 40.0%
! TGTACCATTGGATGATTTGG+AGG + chr4.2:82961386-82961405 MS.gene92590:CDS 40.0%
!! AAAGTGTTGCAAAACTCTCC+TGG + chr4.2:82959649-82959668 MS.gene92590:CDS 40.0%
!! GTTTTAGTCTCTCTCTTGGT+TGG - chr4.2:82960486-82960505 None:intergenic 40.0%
!! TAGATCCAATTGGTGTTGTG+AGG - chr4.2:82960930-82960949 None:intergenic 40.0%
!! TGCAGTTTTAGTCTCTCTCT+TGG - chr4.2:82960490-82960509 None:intergenic 40.0%
!!! CTTGCTCCTAGTTTTAAAGG+AGG - chr4.2:82961713-82961732 None:intergenic 40.0%
GGTTAAGCTAACCAGTGTCT+TGG - chr4.2:82961564-82961583 None:intergenic 45.0%
TCAACATTCTCGCCAAGACA+TGG + chr4.2:82960790-82960809 MS.gene92590:CDS 45.0%
TGCTCCTCCAAATCATCCAA+TGG - chr4.2:82961393-82961412 None:intergenic 45.0%
! AGCCACCTTTGGATTTGGTA+AGG - chr4.2:82961058-82961077 None:intergenic 45.0%
! CCACCTTTGGATTTGGTAAG+GGG - chr4.2:82961056-82961075 None:intergenic 45.0%
! GAGGAGCATATAGTGAGCAT+TGG + chr4.2:82961405-82961424 MS.gene92590:CDS 45.0%
! GCCACCTTTGGATTTGGTAA+GGG - chr4.2:82961057-82961076 None:intergenic 45.0%
! TCCAAAGAGGCTTTGACAGA+AGG + chr4.2:82961796-82961815 MS.gene92590:CDS 45.0%
!! AGTGAGCATTGGAGAAACAG+AGG + chr4.2:82961416-82961435 MS.gene92590:CDS 45.0%
!! CTGCTTTGGGATTTGTGAAG+AGG + chr4.2:82961280-82961299 MS.gene92590:CDS 45.0%
!! TGTCAGGCACTGAACTTGTT+GGG + chr4.2:82961757-82961776 MS.gene92590:intron 45.0%
!! TTGTCAGGCACTGAACTTGT+TGG + chr4.2:82961756-82961775 MS.gene92590:intron 45.0%
CCAAAGGTGGCTCCTCAATT+TGG + chr4.2:82961066-82961085 MS.gene92590:CDS 50.0%
CCAAATTGAGGAGCCACCTT+TGG - chr4.2:82961069-82961088 None:intergenic 50.0%
CCCCTTACCAAATCCAAAGG+TGG + chr4.2:82961053-82961072 MS.gene92590:CDS 50.0%
GCCTTCTGTCAAAGCCTCTT+TGG - chr4.2:82961800-82961819 None:intergenic 50.0%
TGAGGAGCCACCTTTGGATT+TGG - chr4.2:82961063-82961082 None:intergenic 50.0%
AACCAGTGTCTTGGGGACAC+CGG - chr4.2:82961555-82961574 None:intergenic 55.0%
TCAAGGCGGCTAGTGGTGTT+CGG - chr4.2:82959816-82959835 None:intergenic 55.0%
TTCTCTCTCAAGGCGGCTAG+TGG - chr4.2:82959823-82959842 None:intergenic 55.0%
! TGTCTTGGGGACACCGGTTA+AGG - chr4.2:82961549-82961568 None:intergenic 55.0%
AACCGGTGTCCCCAAGACAC+TGG + chr4.2:82961550-82961569 MS.gene92590:intron 60.0%
CAAGGCGGCTAGTGGTGTTC+GGG - chr4.2:82959815-82959834 None:intergenic 60.0%
! CTTGGGGACACCGGTTAAGG+AGG - chr4.2:82961546-82961565 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 82959553 82961864 82959553 ID=MS.gene92590
chr4.2 mRNA 82959553 82961864 82959553 ID=MS.gene92590.t1;Parent=MS.gene92590
chr4.2 exon 82959553 82959756 82959553 ID=MS.gene92590.t1.exon1;Parent=MS.gene92590.t1
chr4.2 CDS 82959553 82959756 82959553 ID=cds.MS.gene92590.t1;Parent=MS.gene92590.t1
chr4.2 exon 82960706 82961437 82960706 ID=MS.gene92590.t1.exon2;Parent=MS.gene92590.t1
chr4.2 CDS 82960706 82961437 82960706 ID=cds.MS.gene92590.t1;Parent=MS.gene92590.t1
chr4.2 exon 82961763 82961864 82961763 ID=MS.gene92590.t1.exon3;Parent=MS.gene92590.t1
chr4.2 CDS 82961763 82961864 82961763 ID=cds.MS.gene92590.t1;Parent=MS.gene92590.t1
Gene Sequence

>MS.gene92590

ATGAAGTTGCATGGAGATTTTGGTGAAGAGAGTTATAATTTTGATGATGGTGTTTTTGTTAAGAAAGGAAGTTTGTATTTGATTTTTGATGATTTGAAAGTGTTGCAAAACTCTCCTGGTAATTCAGTTCAGCAACTCATTCAACTTGGATACAAAAACTTTCACAAGTTGACACAAGTGTCCTTGAATGTTGGATTGAAAGAGGTAATATCACTTCTATAAATTTTTAATATATTTTTAGGAAAATGTTTGGTGAACACCCGAACACCACTAGCCGCCTTGAGAGAGAAAAAAATAGAGAGAGTGATGTGATTAAAAGATAGAGAGAGCGAGAATGTTTGGCTTATATATAGTTTTGGTCTTTCTATTTTGTTACATTTATGATTGTGATCTTTATTTTTAAATTCATGAATTTGACCAGTTAATTTACAAAATATCAGATGTTTTCTCCACCTATGTATTTAGGGTCAATTTTTCATGGTATTTATATGGTAATTAAATTGGTAACATGAATATGAGTTATAAAATAAGAATACAATGTTTTACATCAAATTTTTTTCTTTATATAGATTAATGCATATTTTTATGAAATGAAAGTTTTTTTTAAAAAAAAGTCCGTGTAGAAATATCACGTCATCATTAATTAAGTTGGCACGTAAACATCCCACTTGTCACATCATCAAAATTCTAATTTAAATGTGAGGAAATCATAGTCATTTTAAAAACTGACTTTAAAAATCGTGGATTTGGAAAAAGCAAATGGACCAATGTCACAAATTAGACAAAATAGAGAGGGACGGAGTTCAGTCCCATAAGTGTAGCTCAATTGATAGTTACCAAGGATTTTACGTGTAGAAGTTGGAGTTCGAACCCCGAATTCTTCACTTTTCTACATATAATGTGTGTGAATTTAGTCATTAGACTACTTGACCAACCAAGAGAGAGACTAAAACTGCATTTAAACAAAAAATAATAATTTAGACATGGATTTCTGATTAATGTTTGTAAGACGAGCTTTATAATATATATACTTCCTTAAGTTCTTTAATTAATTTTTAAACATGTGTATGGGAGATATGTGAAACAAGTGTTAGAATGTAATATAAAAATTTCTTAATTTAACAATATGTTTGTGCCTATTTTAACATTCCAGATTTTGGACTTGCTAAAGCAATCATTAATCTCCAAGTCTCCTCTCACTGATATATTTTTGGCCAATGAAGAATTTAAAATGATGTCAACATTCTCGCCAAGACATGGACCAAAATTTGAAAAATGGACTAATATAAAACTTAAGGTAATGGTGAGAAAATCAAAGAAGAAGATACTATATGCTGAAGCTGAGGGAGAATTTGTTGATTTTCTATTAAGTTTCCTCACAACACCAATTGGATCTATTCTAGGACAATTGAATGACAATTTTTCTCTAGGATGTGTGGAAAATTTGCACAAAAGTGTGAAAGAATTCAATCCTTCATGGTTCATAAGGCCTTTAGGTAACCCCTTACCAAATCCAAAGGTGGCTCCTCAATTTGGTTGCATGAAGCAACCACTAATAAATCTTTGTGAAGAAAAAGCTCCTACTTATTGGTATGGTAATGGTGTGATTAAAAATCATATTTGTTATTCCAATGTGAATGGAGTGATATCAAAGCAAAAAAGTCTTGTAAAAAATCCAGAAGCAATGAAACTTTTTGATCCAAGATCACCTTATGGCACAATTGAATCTGCTTTGGGATTTGTGAAGAGGCCATCACTATTTATTGTGTGGGATGATTTGCATGTGACACCATTTGCAAATACTTCAAGCATATCATTTTTGCATAAGATGAATGTACCATTGGATGATTTGGAGGAGCATATAGTGAGCATTGGAGAAACAGAGGTAACAATCATTATTAATTAATTAATTTATCTTGTCCTGGCAAATCTTGATACTTGGATATTTAATGATTATCTTGTTTTCAACTCAAAACATGCATTTTTTTTACCTCCTTAACCGGTGTCCCCAAGACACTGGTTAGCTTAACCAAAAAAATTTAACAAGAAAATAGATGTTAAGGTAGACATGTATAAAATAGATAAAAGTTTATTATATATATATATGTGTGTGTGTGTGGTTTACTCATTATAAAATTAGGTGGAATTTTCTATACACCTCCTTTAAAACTAGGAGCAAGTAATGATTTTAATTATCTCTATATTGTCAGGCACTGAACTTGTTGGGAGCTTCTTTGTCTTCTTCCAAAGAGGCTTTGACAGAAGGCTTGTTCTACCTCCTTAAGAATCCAAAGGAAGATGTTACAGCTTGA
Protein sequence

>MS.gene92590.t1

MKLHGDFGEESYNFDDGVFVKKGSLYLIFDDLKVLQNSPGNSVQQLIQLGYKNFHKLTQVSLNVGLKEILDLLKQSLISKSPLTDIFLANEEFKMMSTFSPRHGPKFEKWTNIKLKVMVRKSKKKILYAEAEGEFVDFLLSFLTTPIGSILGQLNDNFSLGCVENLHKSVKEFNPSWFIRPLGNPLPNPKVAPQFGCMKQPLINLCEEKAPTYWYGNGVIKNHICYSNVNGVISKQKSLVKNPEAMKLFDPRSPYGTIESALGFVKRPSLFIVWDDLHVTPFANTSSISFLHKMNVPLDDLEEHIVSIGETEALNLLGASLSSSKEALTEGLFYLLKNPKEDVTA