AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007916.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007916.01.T01 MTR_2g035320 96.815 157 5 0 1 157 1 157 1.01e-107 304
MsG0280007916.01.T01 MTR_2g035190 94.268 157 9 0 1 157 1 157 8.75e-105 296
MsG0280007916.01.T01 MTR_2g035210 89.809 157 16 0 1 157 1 157 1.32e-100 286
MsG0280007916.01.T01 MTR_2g035220 86.624 157 21 0 1 157 1 157 2.01e-96 275
MsG0280007916.01.T01 MTR_6g033450 64.968 157 55 0 1 157 1 157 4.18e-70 209
MsG0280007916.01.T01 MTR_4g120970 64.968 157 55 0 1 157 1 157 4.18e-70 209
MsG0280007916.01.T01 MTR_2g035100 63.694 157 57 0 1 157 1 157 8.65e-68 203
MsG0280007916.01.T01 MTR_2g035105 63.057 157 58 0 1 157 1 157 8.81e-67 200
MsG0280007916.01.T01 MTR_2g035130 61.783 157 60 0 1 157 1 157 8.05e-66 198
MsG0280007916.01.T01 MTR_2g035150 61.783 157 60 0 1 157 1 157 2.77e-65 196
MsG0280007916.01.T01 MTR_2g035320 93.000 100 7 0 58 157 23 122 1.34e-62 188
MsG0280007916.01.T01 MTR_2g035120 56.051 157 69 0 1 157 1 157 1.67e-59 182
MsG0280007916.01.T01 MTR_2g035170 45.860 157 85 0 1 157 1 157 7.24e-47 150
MsG0280007916.01.T01 MTR_4g120950 42.236 161 86 5 1 157 1 158 5.71e-32 112
MsG0280007916.01.T01 MTR_2g435310 32.051 156 105 1 1 155 1 156 6.96e-30 107
MsG0280007916.01.T01 MTR_4g120760 33.121 157 104 1 1 156 1 157 5.38e-29 104
MsG0280007916.01.T01 MTR_4g120760 33.121 157 104 1 1 156 1 157 5.38e-29 104
MsG0280007916.01.T01 MTR_4g120940 61.644 73 28 0 85 157 47 119 5.80e-24 90.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 42 sgRNAs with CRISPR-Local

Find 62 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAATGGATAACTTCTTAA+TGG 0.186795 2:-22878195 None:intergenic
CTTCAACAATTTCAACAGTT+TGG 0.243960 2:-22878154 None:intergenic
GGATAACTTCTTAATGGTTC+CGG 0.295277 2:-22878189 None:intergenic
TTCAACAATTTCAACAGTTT+GGG 0.345097 2:-22878153 None:intergenic
TACAGCTTAGTAGGAGGAAC+AGG 0.356588 2:+22878744 MsG0280007916.01.T01:CDS
GTTGAAATTGTTGAAGGAAA+TGG 0.360922 2:+22878161 MsG0280007916.01.T01:CDS
GAAACTAAGGCCAGAGGAAC+TGG 0.366866 2:+22878900 MsG0280007916.01.T01:CDS
TGGGCAGCAGAAATCACCTT+TGG 0.390043 2:-22878134 None:intergenic
GATAACTTCTTAATGGTTCC+GGG 0.390267 2:-22878188 None:intergenic
AGAGCTTTGTAGAGTTTAGC+TGG 0.394931 2:-22878086 None:intergenic
TGCAGTAGATGAGGCAAACT+TGG 0.396721 2:+22878713 MsG0280007916.01.T01:CDS
CTTGATAAGTCCAGTTCCTC+TGG 0.419380 2:-22878910 None:intergenic
GGATACAACTACAGCTTAGT+AGG 0.431725 2:+22878735 MsG0280007916.01.T01:CDS
AGATAGAGCTGCATCACCTT+TGG 0.442558 2:-22878862 None:intergenic
CTCTTAATTACCTTCAACAA+TGG 0.448029 2:-22878210 None:intergenic
GGGCAGCAGAAATCACCTTT+GGG 0.452332 2:-22878133 None:intergenic
AAGAAGTTATCCATTGTTGA+AGG 0.479062 2:+22878200 MsG0280007916.01.T01:CDS
AAAATTGTGGCTGGCTCTGA+TGG 0.498760 2:+22878804 MsG0280007916.01.T01:CDS
CAAACTGTTGAAATTGTTGA+AGG 0.498891 2:+22878155 MsG0280007916.01.T01:CDS
TAATTTGTGTAGCACAAAGT+TGG 0.501990 2:-22878688 None:intergenic
CAGAGGAACTGGACTTATCA+AGG 0.507213 2:+22878911 MsG0280007916.01.T01:CDS
GGACTTATCAAGGCCATTGA+GGG 0.514208 2:+22878921 MsG0280007916.01.T01:CDS
TTTCACTGTAATCTTAGCAA+TGG 0.523574 2:-22878832 None:intergenic
CTCTGGCCTTAGTTTCATCA+CGG 0.532674 2:-22878893 None:intergenic
TGCAGTCCGTGATGAAACTA+AGG 0.533307 2:+22878887 MsG0280007916.01.T01:CDS
TGCCACGACGTAGCCCTCAA+TGG 0.549026 2:-22878934 None:intergenic
TGGACTTATCAAGGCCATTG+AGG 0.572485 2:+22878920 MsG0280007916.01.T01:CDS
CAAATTAGATGCAGTAGATG+AGG 0.587202 2:+22878704 MsG0280007916.01.T01:CDS
ATTGTGGCTGGCTCTGATGG+TGG 0.588345 2:+22878807 MsG0280007916.01.T01:CDS
GCAGTAGATGAGGCAAACTT+GGG 0.598362 2:+22878714 MsG0280007916.01.T01:CDS
GTTGAAGGAAATGGAGGACC+CGG 0.599694 2:+22878170 MsG0280007916.01.T01:CDS
GTAGAGTTTAGCTGGAGCCA+CGG 0.602676 2:-22878078 None:intergenic
TGCTGATGAAATCGTCCCAA+AGG 0.606260 2:+22878118 MsG0280007916.01.T01:CDS
CTTAGTAGGAGGAACAGGGT+TGG 0.609437 2:+22878749 MsG0280007916.01.T01:CDS
GAAATTGTTGAAGGAAATGG+AGG 0.626795 2:+22878164 MsG0280007916.01.T01:CDS
TACAACTACAGCTTAGTAGG+AGG 0.637578 2:+22878738 MsG0280007916.01.T01:CDS
GGCCATTGAGGGCTACGTCG+TGG 0.646344 2:+22878932 MsG0280007916.01.T01:CDS
GAGCTGCATCACCTTTGGTG+TGG 0.654393 2:-22878857 None:intergenic
ACAGTGAAATACCACACCAA+AGG 0.661287 2:+22878846 MsG0280007916.01.T01:CDS
CGTGATGAAACTAAGGCCAG+AGG 0.688505 2:+22878894 MsG0280007916.01.T01:CDS
TGATGAACATGTATCAACCG+TGG 0.718349 2:+22878061 MsG0280007916.01.T01:CDS
ACAGCTTAGTAGGAGGAACA+GGG 0.723853 2:+22878745 MsG0280007916.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATATAAATAATTATAA+TGG + Chr2:22878645-22878664 MsG0280007916.01.T01:intron 0.0%
!! AAATAAGTTAAATTCATTAT+GGG + Chr2:22878438-22878457 MsG0280007916.01.T01:intron 10.0%
!!! TTTGTCAATTTTTAAATAAA+AGG - Chr2:22878359-22878378 None:intergenic 10.0%
!! AATAAGTTAAATTCATTATG+GGG + Chr2:22878439-22878458 MsG0280007916.01.T01:intron 15.0%
!! CAAATAAGTTAAATTCATTA+TGG + Chr2:22878437-22878456 MsG0280007916.01.T01:intron 15.0%
!!! GACTTTAAAAAAAATGATTC+AGG + Chr2:22878541-22878560 MsG0280007916.01.T01:intron 20.0%
! AACAATGGATAACTTCTTAA+TGG - Chr2:22878198-22878217 None:intergenic 25.0%
! ATAATTATTAGACATACCCA+TGG - Chr2:22878520-22878539 None:intergenic 25.0%
! ATGGGTATGTCTAATAATTA+TGG + Chr2:22878519-22878538 MsG0280007916.01.T01:intron 25.0%
! ATTACATCATTCATTTCTCA+TGG - Chr2:22878484-22878503 None:intergenic 25.0%
! TGAATTCGAAACAAAAATTG+TGG + Chr2:22878791-22878810 MsG0280007916.01.T01:CDS 25.0%
! TTCAACAATTTCAACAGTTT+GGG - Chr2:22878156-22878175 None:intergenic 25.0%
AAGAAGTTATCCATTGTTGA+AGG + Chr2:22878200-22878219 MsG0280007916.01.T01:CDS 30.0%
CAAACTGTTGAAATTGTTGA+AGG + Chr2:22878155-22878174 MsG0280007916.01.T01:CDS 30.0%
CTCTTAATTACCTTCAACAA+TGG - Chr2:22878213-22878232 None:intergenic 30.0%
CTTCAACAATTTCAACAGTT+TGG - Chr2:22878157-22878176 None:intergenic 30.0%
GTTGAAATTGTTGAAGGAAA+TGG + Chr2:22878161-22878180 MsG0280007916.01.T01:CDS 30.0%
TATAATGGTTGTATTGCAGA+TGG + Chr2:22878660-22878679 MsG0280007916.01.T01:intron 30.0%
TGGGGTATGTCTAATAATTA+TGG + Chr2:22878457-22878476 MsG0280007916.01.T01:intron 30.0%
TTTCACTGTAATCTTAGCAA+TGG - Chr2:22878835-22878854 None:intergenic 30.0%
! TAATTTGTGTAGCACAAAGT+TGG - Chr2:22878691-22878710 None:intergenic 30.0%
!! AAGATCGATTTGAACTTTAC+CGG - Chr2:22878580-22878599 None:intergenic 30.0%
AATACATAAGCTCGTTCCAT+GGG + Chr2:22878501-22878520 MsG0280007916.01.T01:intron 35.0%
CAAATTAGATGCAGTAGATG+AGG + Chr2:22878704-22878723 MsG0280007916.01.T01:CDS 35.0%
GAAATTGTTGAAGGAAATGG+AGG + Chr2:22878164-22878183 MsG0280007916.01.T01:CDS 35.0%
GATAACTTCTTAATGGTTCC+GGG - Chr2:22878191-22878210 None:intergenic 35.0%
GGATAACTTCTTAATGGTTC+CGG - Chr2:22878192-22878211 None:intergenic 35.0%
TAATACATAAGCTCGTTCCA+TGG + Chr2:22878500-22878519 MsG0280007916.01.T01:intron 35.0%
TTCAGGTGATTAATGATACC+CGG + Chr2:22878558-22878577 MsG0280007916.01.T01:intron 35.0%
TTCGAAACAAAAATTGTGGC+TGG + Chr2:22878795-22878814 MsG0280007916.01.T01:CDS 35.0%
! TACACAATTCTTTGTTGTGC+TGG - Chr2:22878332-22878351 None:intergenic 35.0%
!! AGATCGATTTGAACTTTACC+GGG - Chr2:22878579-22878598 None:intergenic 35.0%
ACAGTGAAATACCACACCAA+AGG + Chr2:22878846-22878865 MsG0280007916.01.T01:CDS 40.0%
GGATACAACTACAGCTTAGT+AGG + Chr2:22878735-22878754 MsG0280007916.01.T01:CDS 40.0%
TACAACTACAGCTTAGTAGG+AGG + Chr2:22878738-22878757 MsG0280007916.01.T01:CDS 40.0%
TGATGAACATGTATCAACCG+TGG + Chr2:22878061-22878080 MsG0280007916.01.T01:CDS 40.0%
! AGAGCTTTGTAGAGTTTAGC+TGG - Chr2:22878089-22878108 None:intergenic 40.0%
ACAGCTTAGTAGGAGGAACA+GGG + Chr2:22878745-22878764 MsG0280007916.01.T01:CDS 45.0%
AGATAGAGCTGCATCACCTT+TGG - Chr2:22878865-22878884 None:intergenic 45.0%
CAGAGGAACTGGACTTATCA+AGG + Chr2:22878911-22878930 MsG0280007916.01.T01:CDS 45.0%
CTTGATAAGTCCAGTTCCTC+TGG - Chr2:22878913-22878932 None:intergenic 45.0%
GCAGTAGATGAGGCAAACTT+GGG + Chr2:22878714-22878733 MsG0280007916.01.T01:CDS 45.0%
GGACTTATCAAGGCCATTGA+GGG + Chr2:22878921-22878940 MsG0280007916.01.T01:CDS 45.0%
GGCGAACTTTGATCAGTTGT+CGG - Chr2:22878311-22878330 None:intergenic 45.0%
TACAGCTTAGTAGGAGGAAC+AGG + Chr2:22878744-22878763 MsG0280007916.01.T01:CDS 45.0%
TGCAGTAGATGAGGCAAACT+TGG + Chr2:22878713-22878732 MsG0280007916.01.T01:CDS 45.0%
TGCAGTCCGTGATGAAACTA+AGG + Chr2:22878887-22878906 MsG0280007916.01.T01:CDS 45.0%
TGCTGATGAAATCGTCCCAA+AGG + Chr2:22878118-22878137 MsG0280007916.01.T01:CDS 45.0%
TGGACTTATCAAGGCCATTG+AGG + Chr2:22878920-22878939 MsG0280007916.01.T01:CDS 45.0%
!! AAAATTGTGGCTGGCTCTGA+TGG + Chr2:22878804-22878823 MsG0280007916.01.T01:CDS 45.0%
!! CTCTGGCCTTAGTTTCATCA+CGG - Chr2:22878896-22878915 None:intergenic 45.0%
CGTGATGAAACTAAGGCCAG+AGG + Chr2:22878894-22878913 MsG0280007916.01.T01:CDS 50.0%
CTTAGTAGGAGGAACAGGGT+TGG + Chr2:22878749-22878768 MsG0280007916.01.T01:CDS 50.0%
GAAACTAAGGCCAGAGGAAC+TGG + Chr2:22878900-22878919 MsG0280007916.01.T01:CDS 50.0%
GGGCAGCAGAAATCACCTTT+GGG - Chr2:22878136-22878155 None:intergenic 50.0%
GTAGAGTTTAGCTGGAGCCA+CGG - Chr2:22878081-22878100 None:intergenic 50.0%
GTTGAAGGAAATGGAGGACC+CGG + Chr2:22878170-22878189 MsG0280007916.01.T01:CDS 50.0%
TGGGCAGCAGAAATCACCTT+TGG - Chr2:22878137-22878156 None:intergenic 50.0%
GAGCTGCATCACCTTTGGTG+TGG - Chr2:22878860-22878879 None:intergenic 55.0%
!! ATTGTGGCTGGCTCTGATGG+TGG + Chr2:22878807-22878826 MsG0280007916.01.T01:CDS 55.0%
TGCCACGACGTAGCCCTCAA+TGG - Chr2:22878937-22878956 None:intergenic 60.0%
GGCCATTGAGGGCTACGTCG+TGG + Chr2:22878932-22878951 MsG0280007916.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 22878041 22878971 22878041 ID=MsG0280007916.01;Name=MsG0280007916.01
Chr2 mRNA 22878041 22878971 22878041 ID=MsG0280007916.01.T01;Parent=MsG0280007916.01;Name=MsG0280007916.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|157
Chr2 exon 22878041 22878221 22878041 ID=MsG0280007916.01.T01:exon:13890;Parent=MsG0280007916.01.T01
Chr2 exon 22878679 22878971 22878679 ID=MsG0280007916.01.T01:exon:13891;Parent=MsG0280007916.01.T01
Chr2 CDS 22878041 22878221 22878041 ID=MsG0280007916.01.T01:cds;Parent=MsG0280007916.01.T01
Chr2 CDS 22878679 22878971 22878679 ID=MsG0280007916.01.T01:cds;Parent=MsG0280007916.01.T01
Gene Sequence

>MsG0280007916.01.T01

ATGGGTGTTTTTACTTTCAATGATGAACATGTATCAACCGTGGCTCCAGCTAAACTCTACAAAGCTCTTGCAAAAGATGCTGATGAAATCGTCCCAAAGGTGATTTCTGCTGCCCAAACTGTTGAAATTGTTGAAGGAAATGGAGGACCCGGAACCATTAAGAAGTTATCCATTGTTGAAGATGGCAAAACCAACTTTGTGCTACACAAATTAGATGCAGTAGATGAGGCAAACTTGGGATACAACTACAGCTTAGTAGGAGGAACAGGGTTGGATGAAAGTTTAGAGAAAGTTGAATTCGAAACAAAAATTGTGGCTGGCTCTGATGGTGGATCCATTGCTAAGATTACAGTGAAATACCACACCAAAGGTGATGCAGCTCTATCTGATGCAGTCCGTGATGAAACTAAGGCCAGAGGAACTGGACTTATCAAGGCCATTGAGGGCTACGTCGTGGCAAACCCTAATTACTAA

Protein sequence

>MsG0280007916.01.T01

MGVFTFNDEHVSTVAPAKLYKALAKDADEIVPKVISAAQTVEIVEGNGGPGTIKKLSIVEDGKTNFVLHKLDAVDEANLGYNYSLVGGTGLDESLEKVEFETKIVAGSDGGSIAKITVKYHTKGDAALSDAVRDETKARGTGLIKAIEGYVVANPNY*