AlfalfaGEDB | MsG0280008104.01

M. sativa cultivar ZhongmuNo.1 / MsG0280008104.01

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008104.01.T01	RHN73527.1	96.19	105	4	0	1	105	213	317	6.87E-66	211

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008104.01.T01	A0A396JED4	96.190	105	4	0	1	105	213	317	3.28e-66	211

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000585.01	MsG0280008104.01	0.813677	2.268295e-51	1.490943e-48
MsG0180000669.01	MsG0280008104.01	0.822578	2.198246e-53	1.847452e-50
MsG0180000680.01	MsG0280008104.01	0.814437	1.541480e-51	1.034318e-48
MsG0180001043.01	MsG0280008104.01	0.804455	2.143105e-49	1.105442e-46
MsG0180001712.01	MsG0280008104.01	0.811090	8.330504e-51	5.109594e-48
MsG0180001921.01	MsG0280008104.01	0.818126	2.306550e-52	1.711732e-49
MsG0180002075.01	MsG0280008104.01	0.800147	1.651662e-48	7.633956e-46
MsG0180002894.01	MsG0280008104.01	0.805367	1.381436e-49	7.295239e-47
MsG0180003252.01	MsG0280008104.01	0.804935	1.700826e-49	8.883427e-47
MsG0180004388.01	MsG0280008104.01	0.815531	8.814809e-52	6.092955e-49
MsG0180005125.01	MsG0280008104.01	0.806685	7.296875e-50	3.985439e-47
MsG0180005268.01	MsG0280008104.01	0.811101	8.285088e-51	5.083160e-48
MsG0180005306.01	MsG0280008104.01	0.819416	1.174618e-52	9.033587e-50
MsG0180005944.01	MsG0280008104.01	0.818175	2.247833e-52	1.670437e-49
MsG0180006016.01	MsG0280008104.01	0.803898	2.797781e-49	1.422746e-46
MsG0280006302.01	MsG0280008104.01	-0.803379	3.585377e-49	1.798955e-46
MsG0280007549.01	MsG0280008104.01	0.808025	3.792611e-50	2.145065e-47
MsG0280007585.01	MsG0280008104.01	0.807341	5.300012e-50	2.944623e-47
MsG0280007710.01	MsG0280008104.01	0.800352	1.500848e-48	6.973185e-46
MsG0280007714.01	MsG0280008104.01	0.800073	1.709820e-48	7.888013e-46
MsG0280007826.01	MsG0280008104.01	0.802413	5.676884e-49	2.779163e-46
MsG0280008104.01	MsG0280008220.01	0.805403	1.357406e-49	7.175061e-47
MsG0280008104.01	MsG0280010614.01	0.818876	1.559167e-52	1.181332e-49
MsG0280008104.01	MsG0280010900.01	0.812812	3.511523e-51	2.254601e-48
MsG0280008104.01	MsG0280011235.01	0.800021	1.751762e-48	8.070809e-46
MsG0280008104.01	MsG0280011293.01	0.802237	6.172256e-49	3.008017e-46
MsG0280008104.01	MsG0280011446.01	0.806157	9.429390e-50	5.081561e-47
MsG0280008104.01	MsG0380011663.01	0.809084	2.253716e-50	1.310784e-47
MsG0280008104.01	MsG0380011754.01	0.809194	2.134216e-50	1.244976e-47
MsG0280008104.01	MsG0380014880.01	0.807764	4.310762e-50	2.421422e-47
MsG0280008104.01	MsG0380015149.01	0.810219	1.285431e-50	7.703353e-48
MsG0280008104.01	MsG0380016859.01	0.817429	3.313179e-52	2.412325e-49
MsG0280008104.01	MsG0380017286.01	0.822756	1.997511e-53	1.687257e-50
MsG0280008104.01	MsG0380017403.01	0.803170	3.961876e-49	1.977298e-46
MsG0280008104.01	MsG0480018635.01	0.819400	1.184903e-52	9.108232e-50
MsG0280008104.01	MsG0480018640.01	0.813462	2.528671e-51	1.652523e-48
MsG0280008104.01	MsG0480018970.01	0.803621	3.195073e-49	1.613149e-46
MsG0280008104.01	MsG0480019373.01	0.816545	5.232114e-52	3.718185e-49
MsG0280008104.01	MsG0480020445.01	0.824826	6.539370e-54	5.857833e-51
MsG0280008104.01	MsG0480020719.01	0.800713	1.266489e-48	5.938169e-46
MsG0280008104.01	MsG0480020720.01	0.822108	2.825697e-53	2.342853e-50
MsG0280008104.01	MsG0480020843.01	0.801108	1.051861e-48	4.981949e-46
MsG0280008104.01	MsG0480023377.01	0.806908	6.545235e-50	3.595722e-47
MsG0280008104.01	MsG0480023378.01	0.802813	4.694987e-49	2.322064e-46
MsG0280008104.01	MsG0480023517.01	0.824319	8.608669e-54	7.598825e-51
MsG0280008104.01	MsG0480023554.01	0.844603	6.824644e-59	1.107529e-55
MsG0280008104.01	MsG0580024114.01	0.837172	6.080018e-57	7.833489e-54
MsG0280008104.01	MsG0580025278.01	0.817916	2.573128e-52	1.898592e-49
MsG0280008104.01	MsG0580027034.01	0.813310	2.731272e-51	1.777425e-48
MsG0280008104.01	MsG0580029629.01	0.812214	4.744482e-51	2.998614e-48
MsG0280008104.01	MsG0580029794.01	0.813628	2.324429e-51	1.525830e-48
MsG0280008104.01	MsG0580030235.01	0.825180	5.395042e-54	4.881893e-51
MsG0280008104.01	MsG0680030685.01	0.801541	8.576917e-49	4.106633e-46
MsG0280008104.01	MsG0680031459.01	0.813138	2.979017e-51	1.929475e-48
MsG0280008104.01	MsG0680032641.01	0.814682	1.360567e-51	9.189538e-49
MsG0280008104.01	MsG0680033926.01	0.801057	1.077650e-48	5.097524e-46
MsG0280008104.01	MsG0680035844.01	0.817038	4.056853e-52	2.922280e-49
MsG0280008104.01	MsG0680035854.01	0.802323	5.924799e-49	2.893916e-46
MsG0280008104.01	MsG0780038495.01	0.817242	3.649595e-52	2.643549e-49
MsG0280008104.01	MsG0780039405.01	0.802116	6.533688e-49	3.174331e-46
MsG0280008104.01	MsG0780039777.01	0.803686	3.096279e-49	1.565903e-46
MsG0280008104.01	MsG0780039875.01	0.812341	4.451229e-51	2.822909e-48
MsG0280008104.01	MsG0780040258.01	0.807611	4.645462e-50	2.598996e-47
MsG0280008104.01	MsG0780040418.01	0.800699	1.274835e-48	5.975235e-46
MsG0280008104.01	MsG0780040758.01	0.817735	2.826729e-52	2.075463e-49
MsG0280008104.01	MsG0780041210.01	0.804721	1.885746e-49	9.794832e-47
MsG0280008104.01	MsG0780041399.01	0.800810	1.210343e-48	5.688905e-46
MsG0280008104.01	MsG0880042053.01	0.822138	2.780110e-53	2.307137e-50
MsG0280008104.01	MsG0880042611.01	0.815253	1.016558e-51	6.972644e-49
MsG0280008104.01	MsG0880044409.01	0.832611	8.563763e-56	9.621434e-53
MsG0280008104.01	MsG0880045106.01	0.817935	2.547165e-52	1.880415e-49
MsG0280008104.01	MsG0880046091.01	0.815203	1.042623e-51	7.141804e-49
MsG0280008104.01	MsG0880046244.01	0.807597	4.676026e-50	2.615130e-47
MsG0280008104.01	MsG0880046329.01	0.803667	3.124555e-49	1.579387e-46
MsG0280008104.01	MsG0880046731.01	0.809963	1.459411e-50	8.688592e-48
MsG0280008104.01	MsG0880046733.01	0.809551	1.789413e-50	1.053795e-47
MsG0280008104.01	MsG0880047019.01	0.805323	1.411368e-49	7.444698e-47
MsG0280008104.01	MsG0880047143.01	0.825806	3.834483e-54	3.532088e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008104.01.T01	MTR_2g039570	94.059	101	6	0	5	105	165	265	4.60e-66	200
MsG0280008104.01.T01	MTR_2g039510	78.788	99	21	0	7	105	175	273	3.50e-54	170
MsG0280008104.01.T01	MTR_2g039480	67.677	99	32	0	7	105	171	269	2.20e-45	147
MsG0280008104.01.T01	MTR_2g039450	70.652	92	27	0	10	101	176	267	7.06e-44	144
MsG0280008104.01.T01	MTR_4g126050	65.625	96	33	0	8	103	227	322	2.02e-41	139
MsG0280008104.01.T01	MTR_4g126120	57.732	97	41	0	1	97	165	261	2.07e-36	124
MsG0280008104.01.T01	MTR_4g126120	57.732	97	41	0	1	97	174	270	3.10e-36	124
MsG0280008104.01.T01	MTR_1g016050	57.471	87	37	0	8	94	167	253	2.79e-30	108
MsG0280008104.01.T01	MTR_1g016060	46.392	97	51	1	8	103	532	628	3.13e-17	75.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280008104.01.T01	AT3G47590	63.441	93	34	0	8	100	216	308	1.83e-37	128
MsG0280008104.01.T01	AT1G29840	60.215	93	37	0	8	100	219	311	1.20e-36	126
MsG0280008104.01.T01	AT3G47560	62.921	89	33	0	6	94	191	279	1.36e-36	125
MsG0280008104.01.T01	AT3G47560	63.218	87	32	0	8	94	167	253	2.87e-36	124
MsG0280008104.01.T01	AT3G47560	62.921	89	33	0	6	94	218	306	3.36e-36	125
MsG0280008104.01.T01	AT3G47560	63.218	87	32	0	8	94	194	280	8.26e-36	123
MsG0280008104.01.T01	AT3G47560	63.218	87	32	0	8	94	203	289	1.00e-35	123
MsG0280008104.01.T01	AT5G11910	56.122	98	41	1	4	101	177	272	6.52e-33	116
MsG0280008104.01.T01	AT5G11910	56.122	98	41	1	4	101	195	290	7.25e-33	116
MsG0280008104.01.T01	AT2G19550	59.302	86	35	0	12	97	168	253	9.39e-33	116

Find 25 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAGCTAACAGTACCCTTATT+AGG	0.182618	2:+25525191	None:intergenic
GGAAACCATTGACCCTAATA+AGG	0.260433	2:-25525204	MsG0280008104.01.T01:CDS
TGTTACCAAGGAAAGTTTAA+TGG	0.285803	2:-25525570	MsG0280008104.01.T01:CDS
ATCTACTGTTGCAGGAAGTT+TGG	0.288030	2:-25525600	MsG0280008104.01.T01:CDS
ATAATATGCAACTTGTGATT+TGG	0.310859	2:+25525296	None:intergenic
TTGAGGACATCTACTGTTGC+AGG	0.350085	2:-25525608	MsG0280008104.01.T01:CDS
AACTCGTGCGCACTTTGAAC+GGG	0.413947	2:+25525332	None:intergenic
AGTACCCTTATTAGGGTCAA+TGG	0.413965	2:+25525199	None:intergenic
AGCTAACAGTACCCTTATTA+GGG	0.419156	2:+25525192	None:intergenic
GCGATCCATTAAACTTTCCT+TGG	0.435831	2:+25525565	None:intergenic
TGCATTCTTTGTCAATCTGC+AGG	0.462188	2:+25525516	None:intergenic
GGCAGGATCCTTACAGTTCA+CGG	0.472167	2:-25525374	MsG0280008104.01.T01:intron
AAACTCGTGCGCACTTTGAA+CGG	0.492795	2:+25525331	None:intergenic
GAAACCATTGACCCTAATAA+GGG	0.495902	2:-25525203	MsG0280008104.01.T01:CDS
CACAAGAACCGTGAACTGTA+AGG	0.511460	2:+25525366	None:intergenic
GGAAAGTTTAATGGATCGCA+TGG	0.521594	2:-25525561	MsG0280008104.01.T01:CDS
ATTTGTTATGAGCTTCATCA+AGG	0.526533	2:-25525225	MsG0280008104.01.T01:CDS
TAATAAGGGTACTGTTAGCT+AGG	0.531240	2:-25525189	None:intergenic
GATGAAGCTCATAACAAATG+AGG	0.542447	2:+25525229	None:intergenic
TTAAACTTTCCTTGGTAACA+CGG	0.555853	2:+25525573	None:intergenic
AAGTGCGCACGAGTTTGCTA+AGG	0.575019	2:-25525324	MsG0280008104.01.T01:CDS
TGCAGATTGACAAAGAATGC+AGG	0.585351	2:-25525514	MsG0280008104.01.T01:intron
TTTGGATTACCGTGTTACCA+AGG	0.615217	2:-25525582	MsG0280008104.01.T01:CDS
ACTCGTGCGCACTTTGAACG+GGG	0.650817	2:+25525333	None:intergenic
GAAAGTTTAATGGATCGCAT+GGG	0.653917	2:-25525560	MsG0280008104.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATAATATGCAACTTGTGATT+TGG	+	Chr2:25525505-25525524	None:intergenic	25.0%
!	TAAGTTTATCAACGAAGAAA+TGG	+	Chr2:25525354-25525373	None:intergenic	25.0%
	ACGCCAAATTACATCATATA+TGG	-	Chr2:25525403-25525422	MsG0280008104.01.T01:intron	30.0%
	CAAATTACATCATATATGGC+AGG	-	Chr2:25525407-25525426	MsG0280008104.01.T01:intron	30.0%
	CTGCCATATATGATGTAATT+TGG	+	Chr2:25525409-25525428	None:intergenic	30.0%
	TGTTACCAAGGAAAGTTTAA+TGG	-	Chr2:25525228-25525247	MsG0280008104.01.T01:CDS	30.0%
!	TTAAACTTTCCTTGGTAACA+CGG	+	Chr2:25525228-25525247	None:intergenic	30.0%
!!	ATTTGTTATGAGCTTCATCA+AGG	-	Chr2:25525573-25525592	MsG0280008104.01.T01:CDS	30.0%
	AGCTAACAGTACCCTTATTA+GGG	+	Chr2:25525609-25525628	None:intergenic	35.0%
	ATTGACAAAGAATGCAGGTT+AGG	-	Chr2:25525289-25525308	MsG0280008104.01.T01:CDS	35.0%
	GAAACCATTGACCCTAATAA+GGG	-	Chr2:25525595-25525614	MsG0280008104.01.T01:CDS	35.0%
	GATGAAGCTCATAACAAATG+AGG	+	Chr2:25525572-25525591	None:intergenic	35.0%
	TAGCTAACAGTACCCTTATT+AGG	+	Chr2:25525610-25525629	None:intergenic	35.0%
!	GAAAGTTTAATGGATCGCAT+GGG	-	Chr2:25525238-25525257	MsG0280008104.01.T01:CDS	35.0%
	AGTACCCTTATTAGGGTCAA+TGG	+	Chr2:25525602-25525621	None:intergenic	40.0%
	ATCTACTGTTGCAGGAAGTT+TGG	-	Chr2:25525198-25525217	MsG0280008104.01.T01:CDS	40.0%
	GCGATCCATTAAACTTTCCT+TGG	+	Chr2:25525236-25525255	None:intergenic	40.0%
	GGAAACCATTGACCCTAATA+AGG	-	Chr2:25525594-25525613	MsG0280008104.01.T01:CDS	40.0%
	TGCAGATTGACAAAGAATGC+AGG	-	Chr2:25525284-25525303	MsG0280008104.01.T01:CDS	40.0%
	TGCATTCTTTGTCAATCTGC+AGG	+	Chr2:25525285-25525304	None:intergenic	40.0%
	TTTGGATTACCGTGTTACCA+AGG	-	Chr2:25525216-25525235	MsG0280008104.01.T01:CDS	40.0%
!	ATCTCTTCTCCTGTTACTTC+TGG	-	Chr2:25525326-25525345	MsG0280008104.01.T01:CDS	40.0%
!	GGAAAGTTTAATGGATCGCA+TGG	-	Chr2:25525237-25525256	MsG0280008104.01.T01:CDS	40.0%
	AAACTCGTGCGCACTTTGAA+CGG	+	Chr2:25525470-25525489	None:intergenic	45.0%
	CACAAGAACCGTGAACTGTA+AGG	+	Chr2:25525435-25525454	None:intergenic	45.0%
	GAAATGGAGCCAGAAGTAAC+AGG	+	Chr2:25525338-25525357	None:intergenic	45.0%
	TTGAGGACATCTACTGTTGC+AGG	-	Chr2:25525190-25525209	MsG0280008104.01.T01:CDS	45.0%
	AACTCGTGCGCACTTTGAAC+GGG	+	Chr2:25525469-25525488	None:intergenic	50.0%
	GGCAGGATCCTTACAGTTCA+CGG	-	Chr2:25525424-25525443	MsG0280008104.01.T01:intron	50.0%
!!	AAGTGCGCACGAGTTTGCTA+AGG	-	Chr2:25525474-25525493	MsG0280008104.01.T01:intron	50.0%
	ACTCGTGCGCACTTTGAACG+GGG	+	Chr2:25525468-25525487	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	25525190	25525630	25525190	ID=MsG0280008104.01;Name=MsG0280008104.01
Chr2	mRNA	25525190	25525630	25525190	ID=MsG0280008104.01.T01;Parent=MsG0280008104.01;Name=MsG0280008104.01.T01;_AED=0.48;_eAED=0.49;_QI=0\|0\|0\|1\|1\|1\|2\|0\|105
Chr2	exon	25525515	25525630	25525515	ID=MsG0280008104.01.T01:exon:34946;Parent=MsG0280008104.01.T01
Chr2	exon	25525190	25525391	25525190	ID=MsG0280008104.01.T01:exon:34945;Parent=MsG0280008104.01.T01
Chr2	CDS	25525515	25525630	25525515	ID=MsG0280008104.01.T01:cds;Parent=MsG0280008104.01.T01
Chr2	CDS	25525190	25525391	25525190	ID=MsG0280008104.01.T01:cds;Parent=MsG0280008104.01.T01

Gene Sequence	>MsG0280008104.01.T01 TTGAGGACATCTACTGTTGCAGGAAGTTTGGATTACCGTGTTACCAAGGAAAGTTTAATGGATCGCATGGGTACAAATATGCATGAAGCGTGCCTGCAGATTGACAAAGAATGCAGGATCCTTACAGTTCACGGTTCTTGTGACAAAATTATCCCCGTTCAAAGTGCGCACGAGTTTGCTAAGGTCATACCAAATCACAAGTTGCATATTATTAAACGAGCTAATCATGCATACAGTAATCATCAAGACGTGTTATCCTCATTTGTTATGAGCTTCATCAAGGAAACCATTGACCCTAATAAGGGTACTGTTAGCTAG
Protein sequence	>MsG0280008104.01.T01 LRTSTVAGSLDYRVTKESLMDRMGTNMHEACLQIDKECRILTVHGSCDKIIPVQSAHEFAKVIPNHKLHIIKRANHAYSNHQDVLSSFVMSFIKETIDPNKGTVS*