AlfalfaGEDB | MsG0480021757.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021757.01

KEGG: -
GO: GO:0009733
PFAM: PF02519

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021757.01.T01	RHN61630.1	98.519	135	2	0	1	135	10	144	2.29E-95	281

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021757.01.T01	Q0V7Z5	50.833	120	57	1	1	118	10	129	3.41E-33	116

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021757.01.T01	A0A396I7Q5	98.519	135	2	0	1	135	10	144	1.09e-95	281

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047945.01	MsG0480021757.01	0.828605	8.192175e-55	8.182504e-52
MsG0080048611.01	MsG0480021757.01	0.809998	1.434191e-50	8.546428e-48
MsG0080049039.01	MsG0480021757.01	0.825024	5.873550e-54	5.291308e-51
MsG0080049053.01	MsG0480021757.01	0.813412	2.594091e-51	1.692908e-48
MsG0080049054.01	MsG0480021757.01	0.810737	9.934759e-51	6.036643e-48
MsG0180000638.01	MsG0480021757.01	0.800547	1.369556e-48	6.394522e-46
MsG0180000878.01	MsG0480021757.01	0.827060	1.927517e-54	1.840348e-51
MsG0180002671.01	MsG0480021757.01	0.806436	8.235224e-50	4.469575e-47
MsG0180003531.01	MsG0480021757.01	0.800854	1.185351e-48	5.578094e-46
MsG0180004163.01	MsG0480021757.01	0.826872	2.137268e-54	2.029417e-51
MsG0180004739.01	MsG0480021757.01	0.812699	3.717566e-51	2.380036e-48
MsG0180005755.01	MsG0480021757.01	0.808384	3.180639e-50	1.816124e-47
MsG0180005961.01	MsG0480021757.01	0.825043	5.811782e-54	5.238530e-51
MsG0180006200.01	MsG0480021757.01	0.829830	4.133478e-55	4.279851e-52
MsG0280010661.01	MsG0480021757.01	0.805144	1.538329e-49	8.077456e-47
MsG0380012801.01	MsG0480021757.01	0.821328	4.280248e-53	3.471663e-50
MsG0380012876.01	MsG0480021757.01	0.826241	3.023013e-54	2.819182e-51
MsG0380013530.01	MsG0480021757.01	0.806045	9.953283e-50	5.348580e-47
MsG0380014729.01	MsG0480021757.01	0.806452	8.168851e-50	4.435595e-47
MsG0480019751.01	MsG0480021757.01	0.818189	2.231754e-52	1.659120e-49
MsG0480019956.01	MsG0480021757.01	0.802361	5.818483e-49	2.844778e-46
MsG0480020350.01	MsG0480021757.01	0.809530	1.807875e-50	1.064077e-47
MsG0480020408.01	MsG0480021757.01	0.800038	1.738344e-48	8.012338e-46
MsG0480021254.01	MsG0480021757.01	0.837965	3.807659e-57	5.027221e-54
MsG0480021303.01	MsG0480021757.01	0.841391	4.889567e-58	7.174256e-55
MsG0480021339.01	MsG0480021757.01	0.807542	4.805249e-50	2.683622e-47
MsG0480021346.01	MsG0480021757.01	0.819263	1.273104e-52	9.749234e-50
MsG0480021521.01	MsG0480021757.01	0.815238	1.024274e-51	7.022721e-49
MsG0480021682.01	MsG0480021757.01	0.804310	2.296460e-49	1.180176e-46
MsG0480021709.01	MsG0480021757.01	0.829246	5.729380e-55	5.831574e-52
MsG0480021754.01	MsG0480021757.01	0.835724	1.420051e-56	1.751519e-53
MsG0480021757.01	MsG0480023216.01	0.805021	1.632467e-49	8.544425e-47
MsG0480021757.01	MsG0480023314.01	0.813373	2.645270e-51	1.724483e-48
MsG0480021757.01	MsG0580024385.01	0.807962	3.910857e-50	2.208268e-47
MsG0480021757.01	MsG0580025513.01	0.801341	9.425124e-49	4.490141e-46
MsG0480021757.01	MsG0580027887.01	0.808309	3.298897e-50	1.879912e-47
MsG0480021757.01	MsG0580027983.01	0.829484	5.015449e-55	5.139993e-52
MsG0480021757.01	MsG0580029753.01	0.831344	1.760763e-55	1.905401e-52
MsG0480021757.01	MsG0680030290.01	0.802501	5.443968e-49	2.670975e-46
MsG0480021757.01	MsG0680030627.01	0.811182	7.954422e-51	4.890778e-48
MsG0480021757.01	MsG0680031715.01	0.828404	9.161966e-55	9.097830e-52
MsG0480021757.01	MsG0780038807.01	0.828775	7.454768e-55	7.482101e-52
MsG0480021757.01	MsG0780039675.01	0.800987	1.113793e-48	5.259258e-46
MsG0480021757.01	MsG0780039746.01	0.809851	1.542168e-50	9.154180e-48
MsG0480021757.01	MsG0780040331.01	0.829145	6.062200e-55	6.151248e-52
MsG0480021757.01	MsG0780041480.01	0.831288	1.817458e-55	1.963372e-52
MsG0480021757.01	MsG0880044228.01	0.802479	5.501855e-49	2.697846e-46
MsG0480021757.01	MsG0880044414.01	0.833079	6.552183e-56	7.465794e-53
MsG0480021757.01	MsG0880046791.01	0.804758	1.852782e-49	9.632539e-47
MsG0480021757.01	MsG0880047536.01	0.810848	9.398390e-51	5.727762e-48
MsG0480021757.01	MsG0880047567.01	0.831486	1.624309e-55	1.765227e-52
MsG0480021757.01	MsG0880047616.01	0.808354	3.226960e-50	1.841165e-47
MsG0380015603.01	MsG0480021757.01	0.818567	1.832767e-52	1.376733e-49
MsG0380016137.01	MsG0480021757.01	0.830672	2.574221e-55	2.730938e-52
MsG0380016957.01	MsG0480021757.01	0.825516	4.491703e-54	4.103209e-51
MsG0380017309.01	MsG0480021757.01	0.803145	4.008124e-49	1.999028e-46
MsG0380017703.01	MsG0480021757.01	0.811600	6.456397e-51	4.013471e-48
MsG0380017966.01	MsG0480021757.01	0.800903	1.158612e-48	5.459106e-46
MsG0380018027.01	MsG0480021757.01	0.827031	1.958561e-54	1.868369e-51
MsG0280006805.01	MsG0480021757.01	0.836339	9.917431e-57	1.246223e-53
MsG0280006825.01	MsG0480021757.01	0.802578	5.250510e-49	2.581051e-46
MsG0280007831.01	MsG0480021757.01	0.838862	2.234189e-57	3.031402e-54
MsG0280008591.01	MsG0480021757.01	0.800537	1.375487e-48	6.420740e-46
MsG0280010376.01	MsG0480021757.01	0.812483	4.144920e-51	2.638633e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021757.01.T01	MTR_4g075760	98.519	135	2	0	1	135	1	135	5.19e-99	280
MsG0480021757.01.T01	MTR_4g072910	40.230	87	45	4	9	92	14	96	4.51e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021757.01.T01	AT1G29450	50.833	120	57	1	1	118	10	129	3.48e-34	116
MsG0480021757.01.T01	AT1G29450	50.833	120	57	1	1	118	10	129	3.48e-34	116
MsG0480021757.01.T01	AT1G29430	50.000	116	55	2	5	119	15	128	7.14e-34	115
MsG0480021757.01.T01	AT1G29420	57.009	107	45	1	1	106	10	116	3.07e-33	114
MsG0480021757.01.T01	AT1G29420	57.009	107	45	1	1	106	50	156	1.29e-32	113
MsG0480021757.01.T01	AT1G29510	54.902	102	45	1	5	105	15	116	4.02e-32	111
MsG0480021757.01.T01	AT5G27780	55.140	107	46	1	1	105	11	117	4.13e-32	111
MsG0480021757.01.T01	AT1G29500	51.429	105	49	1	1	105	10	112	9.52e-32	110
MsG0480021757.01.T01	AT1G29460	42.553	141	71	2	1	133	10	148	9.81e-32	110
MsG0480021757.01.T01	AT1G29460	42.553	141	71	2	1	133	48	186	1.06e-31	111
MsG0480021757.01.T01	AT1G29440	45.385	130	68	2	5	133	14	141	1.52e-29	104
MsG0480021757.01.T01	AT1G29490	52.747	91	42	1	1	90	10	100	1.85e-24	90.5
MsG0480021757.01.T01	AT1G76190	41.333	75	41	1	32	103	23	97	4.38e-15	67.0
MsG0480021757.01.T01	AT1G20470	39.394	66	40	0	30	95	23	88	8.72e-13	61.6
MsG0480021757.01.T01	AT5G66260	39.189	74	44	1	20	92	24	97	2.62e-11	56.6

Find 26 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAACTCTTCATCAGTAAATA+AGG	0.216090	4:-63934614	MsG0480021757.01.T01:CDS
TTCATATCCACTTCGTTTCT+TGG	0.266340	4:+63934634	None:intergenic
AAGATCATGACAGGGATTAA+AGG	0.342898	4:+63934342	None:intergenic
TTAATCCCTGTCATGATCTT+AGG	0.345383	4:-63934339	MsG0480021757.01.T01:CDS
TGTTTAGAGAACTCTTTAAA+TGG	0.360343	4:-63934522	MsG0480021757.01.T01:CDS
AATGAACAAATAATTATCTA+TGG	0.367557	4:-63934310	MsG0480021757.01.T01:CDS
CTATCACACGGCAACATAAT+AGG	0.373785	4:+63934466	None:intergenic
TCATATCCACTTCGTTTCTT+GGG	0.376786	4:+63934635	None:intergenic
CAAAGCCTTCTCCACGTCTT+CGG	0.381612	4:+63934399	None:intergenic
CATTGATCGCAAGCGATTTG+TGG	0.397346	4:-63934572	MsG0480021757.01.T01:CDS
AAAGCCTTCTCCACGTCTTC+GGG	0.423323	4:+63934400	None:intergenic
AACTCTTCATCAGTAAATAA+GGG	0.445037	4:-63934613	MsG0480021757.01.T01:CDS
TTCGTGCTAAGATACTTTAG+AGG	0.450847	4:+63934547	None:intergenic
AAATGGTCCGAGGAAGTGTT+CGG	0.456406	4:-63934505	MsG0480021757.01.T01:CDS
GAGGAAGTGTTCGGATTACC+AGG	0.460604	4:-63934496	MsG0480021757.01.T01:CDS
TGGCGGTCGAAAAGCGCAAG+AGG	0.546936	4:-63934666	MsG0480021757.01.T01:CDS
TGGTAATCCGAACACTTCCT+CGG	0.549919	4:+63934498	None:intergenic
GATTCCCGAAGACGTGGAGA+AGG	0.576460	4:-63934404	MsG0480021757.01.T01:CDS
GTGTTCGGATTACCAGGGAA+TGG	0.590996	4:-63934490	MsG0480021757.01.T01:CDS
AACATAATAGGTCCATTCCC+TGG	0.597389	4:+63934478	None:intergenic
AGAACAGATTCCCGAAGACG+TGG	0.599588	4:-63934410	MsG0480021757.01.T01:CDS
CTCATACCCAAGAAACGAAG+TGG	0.601207	4:-63934641	MsG0480021757.01.T01:CDS
AGAACTCTTTAAATGGTCCG+AGG	0.631606	4:-63934515	MsG0480021757.01.T01:CDS
CATGTATGCTTGTCACAACG+AGG	0.660738	4:-63934368	MsG0480021757.01.T01:CDS
TCCAAGAAAACACTATCACA+CGG	0.685946	4:+63934454	None:intergenic
AGGAAGTGTTCGGATTACCA+GGG	0.722902	4:-63934495	MsG0480021757.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATGAACAAATAATTATCTA+TGG	-	Chr4:63934681-63934700	MsG0480021757.01.T01:CDS	15.0%
!	AACTCTTCATCAGTAAATAA+GGG	-	Chr4:63934378-63934397	MsG0480021757.01.T01:CDS	25.0%
!	TGTTTAGAGAACTCTTTAAA+TGG	-	Chr4:63934469-63934488	MsG0480021757.01.T01:CDS	25.0%
	GAACTCTTCATCAGTAAATA+AGG	-	Chr4:63934377-63934396	MsG0480021757.01.T01:CDS	30.0%
	AAGATCATGACAGGGATTAA+AGG	+	Chr4:63934652-63934671	None:intergenic	35.0%
	TCATATCCACTTCGTTTCTT+GGG	+	Chr4:63934359-63934378	None:intergenic	35.0%
	TCCAAGAAAACACTATCACA+CGG	+	Chr4:63934540-63934559	None:intergenic	35.0%
	TTAATCCCTGTCATGATCTT+AGG	-	Chr4:63934652-63934671	MsG0480021757.01.T01:CDS	35.0%
	TTCATATCCACTTCGTTTCT+TGG	+	Chr4:63934360-63934379	None:intergenic	35.0%
	TTCGTGCTAAGATACTTTAG+AGG	+	Chr4:63934447-63934466	None:intergenic	35.0%
!	TTTTGCCTAAGATCATGACA+GGG	+	Chr4:63934660-63934679	None:intergenic	35.0%
!	TTTTTGCCTAAGATCATGAC+AGG	+	Chr4:63934661-63934680	None:intergenic	35.0%
	AACATAATAGGTCCATTCCC+TGG	+	Chr4:63934516-63934535	None:intergenic	40.0%
	AGAACTCTTTAAATGGTCCG+AGG	-	Chr4:63934476-63934495	MsG0480021757.01.T01:CDS	40.0%
	CTATCACACGGCAACATAAT+AGG	+	Chr4:63934528-63934547	None:intergenic	40.0%
	GAAAAAGTGGCAGAAAATGG+CGG	-	Chr4:63934308-63934327	MsG0480021757.01.T01:CDS	40.0%
	CATGTATGCTTGTCACAACG+AGG	-	Chr4:63934623-63934642	MsG0480021757.01.T01:CDS	45.0%
	CATTGATCGCAAGCGATTTG+TGG	-	Chr4:63934419-63934438	MsG0480021757.01.T01:CDS	45.0%
	CTCATACCCAAGAAACGAAG+TGG	-	Chr4:63934350-63934369	MsG0480021757.01.T01:CDS	45.0%
	GGCGAAAAAGTGGCAGAAAA+TGG	-	Chr4:63934305-63934324	MsG0480021757.01.T01:CDS	45.0%
	TGGTAATCCGAACACTTCCT+CGG	+	Chr4:63934496-63934515	None:intergenic	45.0%
!!	AAATGGTCCGAGGAAGTGTT+CGG	-	Chr4:63934486-63934505	MsG0480021757.01.T01:CDS	45.0%
!!	AGGAAGTGTTCGGATTACCA+GGG	-	Chr4:63934496-63934515	MsG0480021757.01.T01:CDS	45.0%
!!	GCCGTGTGATAGTGTTTTCT+TGG	-	Chr4:63934536-63934555	MsG0480021757.01.T01:CDS	45.0%
	AAAGCCTTCTCCACGTCTTC+GGG	+	Chr4:63934594-63934613	None:intergenic	50.0%
	AGAACAGATTCCCGAAGACG+TGG	-	Chr4:63934581-63934600	MsG0480021757.01.T01:CDS	50.0%
	CAAAGCCTTCTCCACGTCTT+CGG	+	Chr4:63934595-63934614	None:intergenic	50.0%
	GTGTTCGGATTACCAGGGAA+TGG	-	Chr4:63934501-63934520	MsG0480021757.01.T01:CDS	50.0%
!!	GAGGAAGTGTTCGGATTACC+AGG	-	Chr4:63934495-63934514	MsG0480021757.01.T01:CDS	50.0%
	GATTCCCGAAGACGTGGAGA+AGG	-	Chr4:63934587-63934606	MsG0480021757.01.T01:CDS	55.0%
!	TGGCGGTCGAAAAGCGCAAG+AGG	-	Chr4:63934325-63934344	MsG0480021757.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	63934303	63934710	63934303	ID=MsG0480021757.01;Name=MsG0480021757.01
Chr4	mRNA	63934303	63934710	63934303	ID=MsG0480021757.01.T01;Parent=MsG0480021757.01;Name=MsG0480021757.01.T01;_AED=0.29;_eAED=0.29;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|135
Chr4	exon	63934303	63934710	63934303	ID=MsG0480021757.01.T01:exon:7745;Parent=MsG0480021757.01.T01
Chr4	CDS	63934303	63934710	63934303	ID=MsG0480021757.01.T01:cds;Parent=MsG0480021757.01.T01

Gene Sequence

>MsG0480021757.01.T01

ATGGCGAAAAAGTGGCAGAAAATGGCGGTCGAAAAGCGCAAGAGGATCTCATACCCAAGAAACGAAGTGGATATGAACTCTTCATCAGTAAATAAGGGTCACTTTGTTGTTTACAGCATTGATCGCAAGCGATTTGTGGTTCCTCTAAAGTATCTTAGCACGAACGTGTTTAGAGAACTCTTTAAATGGTCCGAGGAAGTGTTCGGATTACCAGGGAATGGACCTATTATGTTGCCGTGTGATAGTGTTTTCTTGGACTATGCAATCTCGTTAGTTCAAGAACAGATTCCCGAAGACGTGGAGAAGGCTTTGATCACTTTCATGTATGCTTGTCACAACGAGGCATCCTTTAATCCCTGTCATGATCTTAGGCAAAAAAATGAACAAATAATTATCTATGGCTTTTGA

Protein sequence

>MsG0480021757.01.T01

MAKKWQKMAVEKRKRISYPRNEVDMNSSSVNKGHFVVYSIDRKRFVVPLKYLSTNVFRELFKWSEEVFGLPGNGPIMLPCDSVFLDYAISLVQEQIPEDVEKALITFMYACHNEASFNPCHDLRQKNEQIIIYGF*