AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023216.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023216.01.T01 MTR_8g089610 93.506 77 5 0 1 77 126 202 2.14e-39 129
MsG0480023216.01.T01 MTR_8g089610 53.623 69 31 1 8 76 60 127 5.86e-11 55.8
MsG0480023216.01.T01 MTR_3g086230 78.481 79 13 3 1 77 120 196 4.07e-26 95.1
MsG0480023216.01.T01 MTR_3g086230 57.333 75 29 2 2 76 50 121 3.68e-18 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023216.01.T01 AT1G67740 83.333 36 6 0 41 76 151 186 1.09e-15 68.2
MsG0480023216.01.T01 AT1G67740 46.479 71 37 1 8 77 45 115 3.37e-13 61.6

Find 22 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGTGGGTTAGCAGCTTC+AGG 0.222861 4:-82277870 MsG0480023216.01.T01:CDS
AAGCTGCTGGTAGTGATAAT+AGG 0.242204 4:-82277797 MsG0480023216.01.T01:CDS
TGGTAGCACCAGCTATTCTT+TGG 0.258357 4:-82277755 MsG0480023216.01.T01:CDS
AAAGGAGTTATCATTGGTTT+AGG 0.300660 4:-82277897 MsG0480023216.01.T01:CDS
AACCGTATGCGTAACGATAA+AGG 0.307985 4:-82277915 None:intergenic
GTTATCATTGGTTTAGGTCT+TGG 0.319441 4:-82277891 MsG0480023216.01.T01:CDS
ACTGTCTCATTCTGTTGATT+TGG 0.347582 4:+82277700 None:intergenic
AGCTGCTGGTAGTGATAATA+GGG 0.355815 4:-82277796 MsG0480023216.01.T01:CDS
GGTAGCACCAGCTATTCTTT+GGG 0.391322 4:-82277754 MsG0480023216.01.T01:CDS
ATTGGTTTAGGTCTTGGTGG+TGG 0.402929 4:-82277885 MsG0480023216.01.T01:CDS
AACGATAAAGGAGTTATCAT+TGG 0.410305 4:-82277903 MsG0480023216.01.T01:CDS
GTGGTGGGTTAGCAGCTTCA+GGG 0.418077 4:-82277869 MsG0480023216.01.T01:CDS
GGGTCAGCTTCTGTTGTTTG+TGG 0.423859 4:-82277775 MsG0480023216.01.T01:CDS
ATCATTGGTTTAGGTCTTGG+TGG 0.458537 4:-82277888 MsG0480023216.01.T01:CDS
TTGGTTTAGGTCTTGGTGGT+GGG 0.471445 4:-82277884 MsG0480023216.01.T01:CDS
CTGTTGATTTGGTTAAGAGC+AGG 0.484319 4:+82277711 None:intergenic
TAAAGAACCCAAAGAATAGC+TGG 0.496379 4:+82277747 None:intergenic
GCTGCAATTGCTGAAGCTGC+TGG 0.514881 4:-82277810 MsG0480023216.01.T01:CDS
TCACTAGCCATTGCATCTTG+TGG 0.532256 4:+82277837 None:intergenic
AGATGCAATGGCTAGTGAAA+TGG 0.532397 4:-82277832 MsG0480023216.01.T01:CDS
AGTGTCACCACAAGATGCAA+TGG 0.617349 4:-82277844 MsG0480023216.01.T01:CDS
GCTGCTGGTAGTGATAATAG+GGG 0.720408 4:-82277795 MsG0480023216.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACGATAAAGGAGTTATCAT+TGG - Chr4:82277704-82277723 MsG0480023216.01.T01:CDS 30.0%
!! AAAGGAGTTATCATTGGTTT+AGG - Chr4:82277710-82277729 MsG0480023216.01.T01:CDS 30.0%
TAAAGAACCCAAAGAATAGC+TGG + Chr4:82277863-82277882 None:intergenic 35.0%
! ACTGTCTCATTCTGTTGATT+TGG + Chr4:82277910-82277929 None:intergenic 35.0%
!! GTTATCATTGGTTTAGGTCT+TGG - Chr4:82277716-82277735 MsG0480023216.01.T01:CDS 35.0%
AGATGCAATGGCTAGTGAAA+TGG - Chr4:82277775-82277794 MsG0480023216.01.T01:CDS 40.0%
! AAGCTGCTGGTAGTGATAAT+AGG - Chr4:82277810-82277829 MsG0480023216.01.T01:CDS 40.0%
! AGCTGCTGGTAGTGATAATA+GGG - Chr4:82277811-82277830 MsG0480023216.01.T01:CDS 40.0%
!! ATCATTGGTTTAGGTCTTGG+TGG - Chr4:82277719-82277738 MsG0480023216.01.T01:CDS 40.0%
!! CTGTTGATTTGGTTAAGAGC+AGG + Chr4:82277899-82277918 None:intergenic 40.0%
AGTGTCACCACAAGATGCAA+TGG - Chr4:82277763-82277782 MsG0480023216.01.T01:CDS 45.0%
GGTAGCACCAGCTATTCTTT+GGG - Chr4:82277853-82277872 MsG0480023216.01.T01:CDS 45.0%
TCACTAGCCATTGCATCTTG+TGG + Chr4:82277773-82277792 None:intergenic 45.0%
TGGTAGCACCAGCTATTCTT+TGG - Chr4:82277852-82277871 MsG0480023216.01.T01:CDS 45.0%
! ATTGGTTTAGGTCTTGGTGG+TGG - Chr4:82277722-82277741 MsG0480023216.01.T01:CDS 45.0%
! GCTGCTGGTAGTGATAATAG+GGG - Chr4:82277812-82277831 MsG0480023216.01.T01:CDS 45.0%
! TTGGTTTAGGTCTTGGTGGT+GGG - Chr4:82277723-82277742 MsG0480023216.01.T01:CDS 45.0%
! GGGTCAGCTTCTGTTGTTTG+TGG - Chr4:82277832-82277851 MsG0480023216.01.T01:CDS 50.0%
GCTGCAATTGCTGAAGCTGC+TGG - Chr4:82277797-82277816 MsG0480023216.01.T01:CDS 55.0%
!! GTGGTGGGTTAGCAGCTTCA+GGG - Chr4:82277738-82277757 MsG0480023216.01.T01:CDS 55.0%
!! GGTGGTGGGTTAGCAGCTTC+AGG - Chr4:82277737-82277756 MsG0480023216.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 82277698 82277931 82277698 ID=MsG0480023216.01;Name=MsG0480023216.01
Chr4 mRNA 82277698 82277931 82277698 ID=MsG0480023216.01.T01;Parent=MsG0480023216.01;Name=MsG0480023216.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|77
Chr4 exon 82277698 82277931 82277698 ID=MsG0480023216.01.T01:exon:23833;Parent=MsG0480023216.01.T01
Chr4 CDS 82277698 82277931 82277698 ID=MsG0480023216.01.T01:cds;Parent=MsG0480023216.01.T01
Gene Sequence

>MsG0480023216.01.T01

ATGCGTAACGATAAAGGAGTTATCATTGGTTTAGGTCTTGGTGGTGGGTTAGCAGCTTCAGGGTTAGTGTCACCACAAGATGCAATGGCTAGTGAAATGGCTGCAATTGCTGAAGCTGCTGGTAGTGATAATAGGGGTCAGCTTCTGTTGTTTGTGGTAGCACCAGCTATTCTTTGGGTTCTTTATAATATTTTGCAGCCTGCTCTTAACCAAATCAACAGAATGAGACAGTGA

Protein sequence

>MsG0480023216.01.T01

MRNDKGVIIGLGLGGGLAASGLVSPQDAMASEMAAIAEAAGSDNRGQLLLFVVAPAILWVLYNILQPALNQINRMRQ*