AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0680030290.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030290.01.T01 MTR_6g004100 88.722 133 13 2 1 133 5 135 7.75e-83 239
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0680030290.01.T01 AT5G23440 48.980 147 48 6 9 133 40 181 5.03e-33 114
MsG0680030290.01.T01 AT5G08410 62.222 90 31 3 44 133 97 183 1.33e-30 108
MsG0680030290.01.T01 AT5G08410 62.222 90 31 3 44 133 117 203 1.73e-30 108

Find 25 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAACAGAACGTGACTTTC+TGG 0.206313 6:+149952 MsG0680030290.01.T01:CDS
CTCTGCTAATCTTCCTTATA+AGG 0.228848 6:+149992 MsG0680030290.01.T01:CDS
GCAACAAATTCAACCTTATA+AGG 0.312698 6:-150005 None:intergenic
CCAAGATTGGAGCGCGTGTT+AGG 0.324986 6:+149856 MsG0680030290.01.T01:CDS
AAGTCACGTTCTGTTTGATT+TGG 0.360970 6:-149947 None:intergenic
CCTAACACGCGCTCCAATCT+TGG 0.382971 6:-149856 None:intergenic
GAGATCAACTTCAGGAACTT+TGG 0.391850 6:-149910 None:intergenic
TGGAGCGCGTGTTAGGGTTA+AGG 0.393600 6:+149863 MsG0680030290.01.T01:CDS
CACTTCTTCAATTGCAACAT+TGG 0.395451 6:+149716 MsG0680030290.01.T01:CDS
AGATCAACTTCAGGAACTTT+GGG 0.402665 6:-149909 None:intergenic
GATTCATCAACAGCCAAGAT+TGG 0.446077 6:+149843 MsG0680030290.01.T01:CDS
CTACCAGTGAGATCAACTTC+AGG 0.479502 6:-149918 None:intergenic
CAAGATTGGAGCGCGTGTTA+GGG 0.489458 6:+149857 MsG0680030290.01.T01:CDS
GGAACATGATAGACTTTGAG+AGG 0.528012 6:-149888 None:intergenic
GTTCCTGAAGTTGATCTCAC+TGG 0.542999 6:+149915 MsG0680030290.01.T01:CDS
ACTCAGATAAGGTGTGATGT+TGG 0.555891 6:+149807 MsG0680030290.01.T01:CDS
TTTGTTGCTGATGATATTCG+AGG 0.560953 6:+150020 MsG0680030290.01.T01:CDS
GTTGATCTCACTGGTAGAGA+AGG 0.573181 6:+149924 MsG0680030290.01.T01:CDS
TAAGAATGACGACTCAGATA+AGG 0.574474 6:+149796 MsG0680030290.01.T01:CDS
AGATGAGCAACAAACTTGAG+AGG 0.590824 6:-150053 None:intergenic
TTCTTCGTATTCATAAACAA+AGG 0.597181 6:-149750 None:intergenic
CAATGTTGCAATTGAAGAAG+TGG 0.598656 6:-149715 None:intergenic
GTATTCATAAACAAAGGTAA+CGG 0.650137 6:-149744 None:intergenic
GATGATATTCGAGGTCCTCG+TGG 0.660929 6:+150029 MsG0680030290.01.T01:CDS
ACAAACTTGAGAGGACCACG+AGG 0.762532 6:-150044 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GTATTCATAAACAAAGGTAA+CGG - Chr6:149747-149766 None:intergenic 25.0%
! TTCTTCGTATTCATAAACAA+AGG - Chr6:149753-149772 None:intergenic 25.0%
GCAACAAATTCAACCTTATA+AGG - Chr6:150008-150027 None:intergenic 30.0%
AAGTCACGTTCTGTTTGATT+TGG - Chr6:149950-149969 None:intergenic 35.0%
AGATCAACTTCAGGAACTTT+GGG - Chr6:149912-149931 None:intergenic 35.0%
CAATGTTGCAATTGAAGAAG+TGG - Chr6:149718-149737 None:intergenic 35.0%
CACTTCTTCAATTGCAACAT+TGG + Chr6:149716-149735 MsG0680030290.01.T01:CDS 35.0%
CTCTGCTAATCTTCCTTATA+AGG + Chr6:149992-150011 MsG0680030290.01.T01:CDS 35.0%
TAAGAATGACGACTCAGATA+AGG + Chr6:149796-149815 MsG0680030290.01.T01:CDS 35.0%
TTTGTTGCTGATGATATTCG+AGG + Chr6:150020-150039 MsG0680030290.01.T01:CDS 35.0%
ACTCAGATAAGGTGTGATGT+TGG + Chr6:149807-149826 MsG0680030290.01.T01:CDS 40.0%
AGATGAGCAACAAACTTGAG+AGG - Chr6:150056-150075 None:intergenic 40.0%
GAGATCAACTTCAGGAACTT+TGG - Chr6:149913-149932 None:intergenic 40.0%
GATTCATCAACAGCCAAGAT+TGG + Chr6:149843-149862 MsG0680030290.01.T01:CDS 40.0%
GGAACATGATAGACTTTGAG+AGG - Chr6:149891-149910 None:intergenic 40.0%
TCAAACAGAACGTGACTTTC+TGG + Chr6:149952-149971 MsG0680030290.01.T01:CDS 40.0%
CTACCAGTGAGATCAACTTC+AGG - Chr6:149921-149940 None:intergenic 45.0%
GTTGATCTCACTGGTAGAGA+AGG + Chr6:149924-149943 MsG0680030290.01.T01:CDS 45.0%
! GTTCCTGAAGTTGATCTCAC+TGG + Chr6:149915-149934 MsG0680030290.01.T01:CDS 45.0%
ACAAACTTGAGAGGACCACG+AGG - Chr6:150047-150066 None:intergenic 50.0%
CAAGATTGGAGCGCGTGTTA+GGG + Chr6:149857-149876 MsG0680030290.01.T01:CDS 50.0%
GATGATATTCGAGGTCCTCG+TGG + Chr6:150029-150048 MsG0680030290.01.T01:CDS 50.0%
CCAAGATTGGAGCGCGTGTT+AGG + Chr6:149856-149875 MsG0680030290.01.T01:CDS 55.0%
CCTAACACGCGCTCCAATCT+TGG - Chr6:149859-149878 None:intergenic 55.0%
TGGAGCGCGTGTTAGGGTTA+AGG + Chr6:149863-149882 MsG0680030290.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr6 gene 149705 150106 149705 ID=MsG0680030290.01;Name=MsG0680030290.01
Chr6 mRNA 149705 150106 149705 ID=MsG0680030290.01.T01;Parent=MsG0680030290.01;Name=MsG0680030290.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|133
Chr6 exon 149705 150106 149705 ID=MsG0680030290.01.T01:exon:1901;Parent=MsG0680030290.01.T01
Chr6 CDS 149705 150106 149705 ID=MsG0680030290.01.T01:cds;Parent=MsG0680030290.01.T01
Gene Sequence

>MsG0680030290.01.T01

ATGACTAGCACCACTTCTTCAATTGCAACATTGGTGACTCCGTTACCTTTGTTTATGAATACGAAGAAGAAGATGATGATTCAGAGAAGCATAAGAATGACGACTCAGATAAGGTGTGATGTTGGAGAAGAAGAACAAGATTCATCAACAGCCAAGATTGGAGCGCGTGTTAGGGTTAAGGTTCCTCTCAAAGTCTATCATGTTCCCAAAGTTCCTGAAGTTGATCTCACTGGTAGAGAAGGCCAAATCAAACAGAACGTGACTTTCTGGAAAGATAATAAGCGAATCTCTGCTAATCTTCCTTATAAGGTTGAATTTGTTGCTGATGATATTCGAGGTCCTCGTGGTCCTCTCAAGTTTGTTGCTCATCTCAAAGATGATGAATTCGAATTTCTAGAATAG

Protein sequence

>MsG0680030290.01.T01

MTSTTSSIATLVTPLPLFMNTKKKMMIQRSIRMTTQIRCDVGEEEQDSSTAKIGARVRVKVPLKVYHVPKVPEVDLTGREGQIKQNVTFWKDNKRISANLPYKVEFVADDIRGPRGPLKFVAHLKDDEFEFLE*