AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580029206.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029206.01.T01 MTR_5g082840 96.774 93 3 0 7 99 1014 1106 1.64e-53 179
MsG0580029206.01.T01 MTR_7g068810 54.444 90 40 1 11 99 1031 1120 3.21e-28 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580029206.01.T01 AT5G05570 54.118 85 39 0 15 99 1040 1124 1.14e-27 105
MsG0580029206.01.T01 AT5G05570 54.118 85 39 0 15 99 1015 1099 1.79e-27 104

Find 30 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAACAACTGAAGAAATTA+AGG 0.301995 5:-93704601 MsG0580029206.01.T01:CDS
TGTAAAGATAAGTTGGGATT+TGG 0.311276 5:-93703539 MsG0580029206.01.T01:three_prime_UTR
GACGAATGGAAAATCGTAAA+TGG 0.325409 5:-93703830 MsG0580029206.01.T01:CDS
TCTGATTCATTTGCTTCTTT+AGG 0.354321 5:-93703697 MsG0580029206.01.T01:three_prime_UTR
TCTTCAGTTGTTCTAGCTCT+TGG 0.355041 5:+93704609 None:intergenic
GACGCAATGTAAAGATAAGT+TGG 0.389597 5:-93703546 MsG0580029206.01.T01:three_prime_UTR
GACAGAATTTCAACTTCTAA+AGG 0.397500 5:+93703753 None:intergenic
AGAAATATTACTGATATTCT+CGG 0.400395 5:+93703488 None:intergenic
GAATATCAGTAATATTTCTA+AGG 0.409740 5:-93703484 MsG0580029206.01.T01:three_prime_UTR
AAGGCTAAATATAGAAAGAC+TGG 0.413685 5:-93704582 MsG0580029206.01.T01:CDS
CGACGGCAGCTGCACTTGCA+AGG 0.452163 5:-93704476 MsG0580029206.01.T01:CDS
TCTAGCAAGTTCACTGGCCA+TGG 0.474330 5:+93703851 None:intergenic
ACGCAATGTAAAGATAAGTT+GGG 0.475958 5:-93703545 MsG0580029206.01.T01:three_prime_UTR
ATACTGAAGAGTTGCAGAAT+GGG 0.482169 5:-93703890 MsG0580029206.01.T01:CDS
TTTGCATGCGCAACTTATTG+AGG 0.485528 5:+93703582 None:intergenic
CATTCGTCTAGCAAGTTCAC+TGG 0.494472 5:+93703845 None:intergenic
CATACTGAAGAGTTGCAGAA+TGG 0.500754 5:-93703891 MsG0580029206.01.T01:CDS
AAATCACTAATAAATTGCAT+AGG 0.541156 5:-93703512 MsG0580029206.01.T01:three_prime_UTR
GACGGCAGCTGCACTTGCAA+GGG 0.562703 5:-93704475 MsG0580029206.01.T01:CDS
TGCAAGTGCAGCTGCCGTCG+AGG 0.566225 5:+93704479 None:intergenic
TCAATAAGTTGCGCATGCAA+AGG 0.579694 5:-93703580 MsG0580029206.01.T01:three_prime_UTR
AGGCTAAATATAGAAAGACT+GGG 0.587018 5:-93704581 MsG0580029206.01.T01:CDS
CTTGCTTCAGGATGCCTCGA+CGG 0.619387 5:-93704493 MsG0580029206.01.T01:intron
CAGTGAACTTGCTAGACGAA+TGG 0.621337 5:-93703844 MsG0580029206.01.T01:CDS
TACTGAAGAGTTGCAGAATG+GGG 0.635999 5:-93703889 MsG0580029206.01.T01:CDS
CAACTTCTAAAGGATTGCAG+CGG 0.646615 5:+93703763 None:intergenic
AGACAGACAGAAGTTACTTG+AGG 0.652763 5:-93704652 MsG0580029206.01.T01:CDS
GAATGGAAAATCGTAAATGG+TGG 0.667397 5:-93703827 MsG0580029206.01.T01:CDS
GGCTAAATATAGAAAGACTG+GGG 0.696264 5:-93704580 MsG0580029206.01.T01:intron
CAAGGGATAAGCTTGTAGAG+CGG 0.731093 5:-93704458 MsG0580029206.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTATTCTTTATTAGA+AGG + Chr5:93704140-93704159 None:intergenic 10.0%
!! CTTGATAAATCAATAATTTA+TGG + Chr5:93703940-93703959 None:intergenic 15.0%
!! GAATAATAATTATTGAAACT+TGG - Chr5:93704149-93704168 MsG0580029206.01.T01:intron 15.0%
!! TTTCATATTCATTAAATAGT+AGG + Chr5:93703793-93703812 None:intergenic 15.0%
!!! ATTTTTTTTTAATAGAGATG+TGG + Chr5:93703983-93704002 None:intergenic 15.0%
!! AAATCACTAATAAATTGCAT+AGG - Chr5:93704629-93704648 MsG0580029206.01.T01:CDS 20.0%
!! AGAAATATTACTGATATTCT+CGG + Chr5:93704656-93704675 None:intergenic 20.0%
!! GAAACCTAATAAACAAAATT+TGG + Chr5:93704185-93704204 None:intergenic 20.0%
!!! ACATAGATTTTTCATGTATA+AGG - Chr5:93703859-93703878 MsG0580029206.01.T01:CDS 20.0%
!!! GAATATCAGTAATATTTCTA+AGG - Chr5:93704657-93704676 MsG0580029206.01.T01:CDS 20.0%
!!! TTCTCCAAATTTTGTTTATT+AGG - Chr5:93704178-93704197 MsG0580029206.01.T01:intron 20.0%
! AAAAATGGAGATTTGTCTTT+CGG + Chr5:93703826-93703845 None:intergenic 25.0%
! AATAATTATTGAAACTTGGC+TGG - Chr5:93704153-93704172 MsG0580029206.01.T01:intron 25.0%
! AATAATTTATGGACTTCCAT+TGG + Chr5:93703929-93703948 None:intergenic 25.0%
! GTCTAGAAACTTCTTAAAAA+TGG + Chr5:93703841-93703860 None:intergenic 25.0%
! TAGAACAACTGAAGAAATTA+AGG - Chr5:93703540-93703559 MsG0580029206.01.T01:three_prime_UTR 25.0%
!! ACGAGAACTTTTATCATTAA+AGG + Chr5:93704018-93704037 None:intergenic 25.0%
!! CGAGAACTTTTATCATTAAA+GGG + Chr5:93704017-93704036 None:intergenic 25.0%
!! GTTCCTTTTTATTATAACTG+AGG - Chr5:93703902-93703921 MsG0580029206.01.T01:CDS 25.0%
!! TTTTTCATGTATAAGGTAAG+TGG - Chr5:93703866-93703885 MsG0580029206.01.T01:CDS 25.0%
AAGGCTAAATATAGAAAGAC+TGG - Chr5:93703559-93703578 MsG0580029206.01.T01:three_prime_UTR 30.0%
AATAGAATAACAGTCTAACC+TGG + Chr5:93703720-93703739 None:intergenic 30.0%
ACGCAATGTAAAGATAAGTT+GGG - Chr5:93704596-93704615 MsG0580029206.01.T01:CDS 30.0%
AGGCTAAATATAGAAAGACT+GGG - Chr5:93703560-93703579 MsG0580029206.01.T01:three_prime_UTR 30.0%
GACAGAATTTCAACTTCTAA+AGG + Chr5:93704391-93704410 None:intergenic 30.0%
GGACCTCAGTTATAATAAAA+AGG + Chr5:93703908-93703927 None:intergenic 30.0%
TATTGTGGAAGGATAAGATT+CGG + Chr5:93703757-93703776 None:intergenic 30.0%
TCATTAAATAGTAGGAGATG+AGG + Chr5:93703785-93703804 None:intergenic 30.0%
TCTGATTCATTTGCTTCTTT+AGG - Chr5:93704444-93704463 MsG0580029206.01.T01:CDS 30.0%
TTATTATAACTGAGGTCCAA+TGG - Chr5:93703910-93703929 MsG0580029206.01.T01:intron 30.0%
! ACTACTTTGATTTCACAAAG+TGG + Chr5:93704111-93704130 None:intergenic 30.0%
! CTACTTTGATTTCACAAAGT+GGG + Chr5:93704110-93704129 None:intergenic 30.0%
! TGTAAAGATAAGTTGGGATT+TGG - Chr5:93704602-93704621 MsG0580029206.01.T01:CDS 30.0%
!!! CTGGTCAATTTTTGGTTATT+TGG + Chr5:93704083-93704102 None:intergenic 30.0%
!!! TTAGCCTCACTTGTTTTTAA+AGG - Chr5:93704417-93704436 MsG0580029206.01.T01:intron 30.0%
AAGATTCGGGTAAAGAAGAA+TGG + Chr5:93703743-93703762 None:intergenic 35.0%
ATACTGAAGAGTTGCAGAAT+GGG - Chr5:93704251-93704270 MsG0580029206.01.T01:intron 35.0%
ATTGTGGAAGGATAAGATTC+GGG + Chr5:93703756-93703775 None:intergenic 35.0%
GAATGGAAAATCGTAAATGG+TGG - Chr5:93704314-93704333 MsG0580029206.01.T01:intron 35.0%
GACGAATGGAAAATCGTAAA+TGG - Chr5:93704311-93704330 MsG0580029206.01.T01:intron 35.0%
GACGCAATGTAAAGATAAGT+TGG - Chr5:93704595-93704614 MsG0580029206.01.T01:CDS 35.0%
GGCTAAATATAGAAAGACTG+GGG - Chr5:93703561-93703580 MsG0580029206.01.T01:three_prime_UTR 35.0%
TCATGTATAAGGTAAGTGGA+TGG - Chr5:93703870-93703889 MsG0580029206.01.T01:CDS 35.0%
! ATCATTAAAGGGTGCAAAAG+TGG + Chr5:93704006-93704025 None:intergenic 35.0%
! TGGGTTAACTGGTCAATTTT+TGG + Chr5:93704091-93704110 None:intergenic 35.0%
!! ATTTCACAAAGTGGGTTAAC+TGG + Chr5:93704102-93704121 None:intergenic 35.0%
AGACAGACAGAAGTTACTTG+AGG - Chr5:93703489-93703508 MsG0580029206.01.T01:three_prime_UTR 40.0%
ATGAGGCTAGATATTGTGGA+AGG + Chr5:93703768-93703787 None:intergenic 40.0%
CATACTGAAGAGTTGCAGAA+TGG - Chr5:93704250-93704269 MsG0580029206.01.T01:intron 40.0%
GATTTGTCTTTCGGTGATTC+AGG + Chr5:93703817-93703836 None:intergenic 40.0%
TACTGAAGAGTTGCAGAATG+GGG - Chr5:93704252-93704271 MsG0580029206.01.T01:intron 40.0%
TCAATAAGTTGCGCATGCAA+AGG - Chr5:93704561-93704580 MsG0580029206.01.T01:intron 40.0%
TTGACCTAATCACTTGCTTC+AGG - Chr5:93703636-93703655 MsG0580029206.01.T01:three_prime_UTR 40.0%
TTTGCATGCGCAACTTATTG+AGG + Chr5:93704562-93704581 None:intergenic 40.0%
! CAACTTCTAAAGGATTGCAG+CGG + Chr5:93704381-93704400 None:intergenic 40.0%
! GCTGCCTTTAAAAACAAGTG+AGG + Chr5:93704424-93704443 None:intergenic 40.0%
!! TCTTCAGTTGTTCTAGCTCT+TGG + Chr5:93703535-93703554 None:intergenic 40.0%
!!! TTTCTTCTGCTTTTTCAGCG+AGG + Chr5:93703468-93703487 None:intergenic 40.0%
CAAGGGATAAGCTTGTAGAG+CGG - Chr5:93703683-93703702 MsG0580029206.01.T01:three_prime_UTR 45.0%
CAGTATGATCGTTGAGCATC+TGG + Chr5:93704237-93704256 None:intergenic 45.0%
CATTCGTCTAGCAAGTTCAC+TGG + Chr5:93704299-93704318 None:intergenic 45.0%
GCATCCTGAAGCAAGTGATT+AGG + Chr5:93703643-93703662 None:intergenic 45.0%
GGAGATGAGGCTAGATATTG+TGG + Chr5:93703772-93703791 None:intergenic 45.0%
GTAAACATGACGAGCAGAGA+TGG + Chr5:93704215-93704234 None:intergenic 45.0%
! GGCACAAGATTTTGCTTCCA+TGG - Chr5:93704273-93704292 MsG0580029206.01.T01:intron 45.0%
!! CAGTGAACTTGCTAGACGAA+TGG - Chr5:93704297-93704316 MsG0580029206.01.T01:intron 45.0%
AGAGCGGCAAGAAAAACTCC+AGG - Chr5:93703699-93703718 MsG0580029206.01.T01:three_prime_UTR 50.0%
GACGAGCAGAGATGGTTAGA+TGG + Chr5:93704207-93704226 None:intergenic 50.0%
TCTAGCAAGTTCACTGGCCA+TGG + Chr5:93704293-93704312 None:intergenic 50.0%
!!! AGCTCTTGGTTTTGCGTCAG+TGG + Chr5:93703521-93703540 None:intergenic 50.0%
!!! TCTTGGTTTTGCGTCAGTGG+AGG + Chr5:93703518-93703537 None:intergenic 50.0%
CTTGCTTCAGGATGCCTCGA+CGG - Chr5:93703648-93703667 MsG0580029206.01.T01:three_prime_UTR 55.0%
GACGGCAGCTGCACTTGCAA+GGG - Chr5:93703666-93703685 MsG0580029206.01.T01:three_prime_UTR 60.0%
CGACGGCAGCTGCACTTGCA+AGG - Chr5:93703665-93703684 MsG0580029206.01.T01:three_prime_UTR 65.0%
TGCAAGTGCAGCTGCCGTCG+AGG + Chr5:93703665-93703684 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 93703454 93704709 93703454 ID=MsG0580029206.01;Name=MsG0580029206.01
Chr5 mRNA 93703454 93704709 93703454 ID=MsG0580029206.01.T01;Parent=MsG0580029206.01;Name=MsG0580029206.01.T01;_AED=0.48;_eAED=0.49;_QI=0|1|0.66|1|1|1|3|364|99
Chr5 exon 93703454 93703925 93703454 ID=MsG0580029206.01.T01:exon:49695;Parent=MsG0580029206.01.T01
Chr5 exon 93704443 93704505 93704443 ID=MsG0580029206.01.T01:exon:49694;Parent=MsG0580029206.01.T01
Chr5 exon 93704581 93704709 93704581 ID=MsG0580029206.01.T01:exon:49693;Parent=MsG0580029206.01.T01
Chr5 CDS 93704581 93704709 93704581 ID=MsG0580029206.01.T01:cds;Parent=MsG0580029206.01.T01
Chr5 CDS 93704443 93704505 93704443 ID=MsG0580029206.01.T01:cds;Parent=MsG0580029206.01.T01
Chr5 CDS 93703818 93703925 93703818 ID=MsG0580029206.01.T01:cds;Parent=MsG0580029206.01.T01
Chr5 three_prime_UTR 93703454 93703817 93703454 ID=MsG0580029206.01.T01:three_prime_utr;Parent=MsG0580029206.01.T01
Gene Sequence

>MsG0580029206.01.T01

ATGAATGGTTACCTCGCTGAAAAAGCAGAAGAAACAGACAGACAGAAGTTACTTGAGGAGTCCTCCACTGACGCAAAACCAAGAGCTAGAACAACTGAAGAAATTAAGGCTAAATATAGAAAGACTGGGGATGCCTCGACGGCAGCTGCACTTGCAAGGGATAAGCTTGTAGAGCGGCAAGAAAAACTCCAGATGCTCAACGATCATACTGAAGAGTTGCAGAATGGGGCACAAGATTTTGCTTCCATGGCCAGTGAACTTGCTAGACGAATGGAAAATCGTAAATGGTGGCAGCTATAA

Protein sequence

>MsG0580029206.01.T01

MNGYLAEKAEETDRQKLLEESSTDAKPRARTTEEIKAKYRKTGDASTAAALARDKLVERQEKLQMLNDHTEELQNGAQDFASMASELARRMENRKWWQL*