AlfalfaGEDB | MsG0280010182.01

M. sativa cultivar ZhongmuNo.1 / MsG0280010182.01

KEGG: -
GO: -
PFAM: PF04935

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010182.01.T01	PNY13417.1	58.537	164	31	2	2	128	104	267	4.35E-47	165

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010182.01.T01	A0A2K3PDP7	58.537	164	31	2	2	128	104	267	2.08e-47	165

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048463.01	MsG0280010182.01	0.809929	1.483956e-50	8.826673e-48
MsG0080048464.01	MsG0280010182.01	0.811636	6.339714e-51	3.944602e-48
MsG0080048618.01	MsG0280010182.01	0.804608	1.990739e-49	1.030935e-46
MsG0080048683.01	MsG0280010182.01	0.836185	1.085338e-56	1.357599e-53
MsG0080048774.01	MsG0280010182.01	0.804368	2.233940e-49	1.149776e-46
MsG0080048826.01	MsG0280010182.01	0.809476	1.856922e-50	1.091340e-47
MsG0080048878.01	MsG0280010182.01	0.801320	9.518106e-49	4.532050e-46
MsG0080048892.01	MsG0280010182.01	0.818968	1.485901e-52	1.128771e-49
MsG0180000334.01	MsG0280010182.01	0.801610	8.300239e-49	3.981043e-46
MsG0180000335.01	MsG0280010182.01	0.823747	1.172385e-53	1.018471e-50
MsG0180000470.01	MsG0280010182.01	0.839445	1.577652e-57	2.179285e-54
MsG0180001063.01	MsG0280010182.01	0.830953	2.196106e-55	2.349134e-52
MsG0180001382.01	MsG0280010182.01	0.809515	1.821699e-50	1.071752e-47
MsG0180002459.01	MsG0280010182.01	0.801048	1.082103e-48	5.117494e-46
MsG0180002547.01	MsG0280010182.01	0.823749	1.171576e-53	1.017802e-50
MsG0180002794.01	MsG0280010182.01	0.807707	4.432444e-50	2.486090e-47
MsG0180003189.01	MsG0280010182.01	0.805943	1.045462e-49	5.603532e-47
MsG0180003673.01	MsG0280010182.01	0.801985	6.954126e-49	3.367227e-46
MsG0180003952.01	MsG0280010182.01	0.822930	1.819627e-53	1.544520e-50
MsG0180004500.01	MsG0280010182.01	0.805734	1.157195e-49	6.169346e-47
MsG0180004765.01	MsG0280010182.01	0.832484	9.208554e-56	1.030665e-52
MsG0180004926.01	MsG0280010182.01	0.808392	3.168394e-50	1.809479e-47
MsG0180004928.01	MsG0280010182.01	0.806971	6.348396e-50	3.493254e-47
MsG0180004997.01	MsG0280010182.01	0.803243	3.824914e-49	1.912499e-46
MsG0180005169.01	MsG0280010182.01	0.814888	1.224944e-51	8.319094e-49
MsG0180005424.01	MsG0280010182.01	0.806657	7.394461e-50	4.035853e-47
MsG0180006096.01	MsG0280010182.01	0.839063	1.982117e-57	2.705889e-54
MsG0180006144.01	MsG0280010182.01	0.803280	3.758406e-49	1.880979e-46
MsG0280006299.01	MsG0280010182.01	0.811167	8.017007e-51	4.927025e-48
MsG0280006306.01	MsG0280010182.01	-0.802846	4.622448e-49	2.288050e-46
MsG0280006309.01	MsG0280010182.01	0.825751	3.951551e-54	3.634138e-51
MsG0280006461.01	MsG0280010182.01	0.807630	4.602222e-50	2.576163e-47
MsG0280006491.01	MsG0280010182.01	0.815464	9.123928e-52	6.295269e-49
MsG0280006593.01	MsG0280010182.01	0.824859	6.423728e-54	5.759707e-51
MsG0280007118.01	MsG0280010182.01	0.836698	8.034374e-57	1.020442e-53
MsG0280007575.01	MsG0280010182.01	0.819359	1.210624e-52	9.294940e-50
MsG0280007727.01	MsG0280010182.01	0.813847	2.080864e-51	1.373999e-48
MsG0280007955.01	MsG0280010182.01	0.832237	1.059939e-55	1.177600e-52
MsG0280008076.01	MsG0280010182.01	0.801651	8.142770e-49	3.909565e-46
MsG0280008112.01	MsG0280010182.01	0.850839	1.309526e-60	2.599260e-57
MsG0280008113.01	MsG0280010182.01	0.836444	9.323727e-57	1.175248e-53
MsG0280008429.01	MsG0280010182.01	0.823223	1.554807e-53	1.330624e-50
MsG0280008928.01	MsG0280010182.01	0.801699	7.959178e-49	3.826049e-46
MsG0280009353.01	MsG0280010182.01	0.806945	6.427472e-50	3.534415e-47
MsG0280009383.01	MsG0280010182.01	0.801859	7.379886e-49	3.562256e-46
MsG0280010013.01	MsG0280010182.01	0.822972	1.779813e-53	1.512452e-50
MsG0280010148.01	MsG0280010182.01	0.821361	4.205378e-53	3.413951e-50
MsG0280010182.01	MsG0280010184.01	0.895938	5.953828e-76	6.302083e-72
MsG0280010182.01	MsG0280010233.01	0.816855	4.459162e-52	3.196001e-49
MsG0280010182.01	MsG0280010349.01	0.818786	1.634205e-52	1.235069e-49
MsG0280010182.01	MsG0280010350.01	0.806053	9.913093e-50	5.328116e-47
MsG0280010182.01	MsG0280010366.01	0.812476	4.160149e-51	2.647864e-48
MsG0280010182.01	MsG0280010434.01	0.812046	5.162293e-51	3.247454e-48
MsG0280010182.01	MsG0280010517.01	0.821296	4.354117e-53	3.528302e-50
MsG0280010182.01	MsG0280010595.01	0.820442	6.844247e-53	5.416252e-50
MsG0280010182.01	MsG0280010722.01	0.839960	1.158868e-57	1.626528e-54
MsG0280010182.01	MsG0280010776.01	0.804534	2.062970e-49	1.066270e-46
MsG0280010182.01	MsG0280010778.01	0.811761	5.956907e-51	3.718899e-48
MsG0280010182.01	MsG0280010796.01	0.804265	2.346976e-49	1.204709e-46
MsG0280010182.01	MsG0280011059.01	-0.804538	2.059228e-49	1.064443e-46
MsG0280010182.01	MsG0280011109.01	0.813173	2.925946e-51	1.896929e-48
MsG0280010182.01	MsG0280011175.01	0.805121	1.555135e-49	8.160940e-47
MsG0280010182.01	MsG0280011339.01	0.811414	7.085609e-51	4.383296e-48
MsG0280010182.01	MsG0280011371.01	0.813298	2.746804e-51	1.786937e-48
MsG0280010182.01	MsG0380011581.01	0.835221	1.903693e-56	2.313015e-53
MsG0280010182.01	MsG0380011609.01	0.820366	7.125284e-53	5.626660e-50
MsG0280010182.01	MsG0380011669.01	0.839535	1.494901e-57	2.070489e-54
MsG0280010182.01	MsG0380012005.01	0.822978	1.774047e-53	1.507839e-50
MsG0280010182.01	MsG0380012570.01	0.808493	3.014904e-50	1.726337e-47
MsG0280010182.01	MsG0380013085.01	0.837383	5.368779e-57	6.961551e-54
MsG0280010182.01	MsG0380014537.01	0.819911	9.056730e-53	7.062327e-50
MsG0280010182.01	MsG0380015166.01	0.803618	3.199419e-49	1.615214e-46
MsG0280010182.01	MsG0380015332.01	0.815998	6.935814e-52	4.855850e-49
MsG0280010182.01	MsG0380015533.01	0.801784	7.648056e-49	3.684444e-46
MsG0280010182.01	MsG0380015555.01	0.823622	1.254099e-53	1.085517e-50
MsG0280010182.01	MsG0380015930.01	0.824565	7.536404e-54	6.700374e-51
MsG0280010182.01	MsG0380016133.01	0.810101	1.362682e-50	8.141972e-48
MsG0280010182.01	MsG0380016191.01	-0.836160	1.101165e-56	1.376226e-53
MsG0280010182.01	MsG0380016285.01	0.836378	9.694793e-57	1.219583e-53
MsG0280010182.01	MsG0380016347.01	-0.811009	8.671876e-51	5.308221e-48
MsG0280010182.01	MsG0380016505.01	0.811965	5.376995e-51	3.375225e-48
MsG0280010182.01	MsG0380016529.01	0.817977	2.491875e-52	1.841676e-49
MsG0280010182.01	MsG0380016560.01	0.807021	6.193481e-50	3.412502e-47
MsG0280010182.01	MsG0380017085.01	0.810143	1.334662e-50	7.982952e-48
MsG0280010182.01	MsG0380017176.01	0.814137	1.795523e-51	1.195016e-48
MsG0280010182.01	MsG0380017198.01	0.821030	5.013544e-53	4.033253e-50
MsG0280010182.01	MsG0380017202.01	0.804128	2.505804e-49	1.281757e-46
MsG0280010182.01	MsG0380017293.01	0.820561	6.426143e-53	5.101952e-50
MsG0280010182.01	MsG0380017461.01	0.829841	4.106314e-55	4.253241e-52
MsG0280010182.01	MsG0380017498.01	0.817121	3.885630e-52	2.805188e-49
MsG0280010182.01	MsG0380017589.01	0.800899	1.160508e-48	5.467588e-46
MsG0280010182.01	MsG0380017947.01	0.804430	2.168658e-49	1.117891e-46
MsG0280010182.01	MsG0480019206.01	0.807889	4.054975e-50	2.285123e-47
MsG0280010182.01	MsG0480020024.01	0.832086	1.155354e-55	1.277949e-52
MsG0280010182.01	MsG0480020025.01	0.809105	2.230485e-50	1.298014e-47
MsG0280010182.01	MsG0480020447.01	0.802465	5.539339e-49	2.715314e-46
MsG0280010182.01	MsG0480020887.01	0.829214	5.835368e-55	5.933643e-52
MsG0280010182.01	MsG0480021056.01	0.825528	4.462896e-54	4.078213e-51
MsG0280010182.01	MsG0480021215.01	0.806669	7.352393e-50	4.014089e-47
MsG0280010182.01	MsG0480021280.01	0.806134	9.535012e-50	5.135367e-47
MsG0280010182.01	MsG0480021536.01	0.810916	9.083537e-51	5.546165e-48
MsG0280010182.01	MsG0480021776.01	-0.811099	8.293331e-51	5.087933e-48
MsG0280010182.01	MsG0480021869.01	0.821644	3.619106e-53	2.961344e-50
MsG0280010182.01	MsG0480021870.01	0.820030	8.504971e-53	6.654421e-50
MsG0280010182.01	MsG0480021925.01	0.818619	1.783044e-52	1.341320e-49
MsG0280010182.01	MsG0480022068.01	0.820027	8.520258e-53	6.665784e-50
MsG0280010182.01	MsG0480022259.01	0.806701	7.238746e-50	3.955447e-47
MsG0280010182.01	MsG0480022985.01	0.821991	3.008385e-53	2.485961e-50
MsG0280010182.01	MsG0480023055.01	0.806103	9.675490e-50	5.206999e-47
MsG0280010182.01	MsG0480023221.01	0.809276	2.049343e-50	1.198077e-47
MsG0280010182.01	MsG0480023489.01	0.804019	2.641129e-49	1.347222e-46
MsG0280010182.01	MsG0480023660.01	0.838861	2.236358e-57	3.034164e-54
MsG0280010182.01	MsG0580024161.01	0.802501	5.444034e-49	2.671005e-46
MsG0280010182.01	MsG0580024495.01	0.813820	2.109185e-51	1.391730e-48
MsG0280010182.01	MsG0580024725.01	0.812953	3.271043e-51	2.108069e-48
MsG0280010182.01	MsG0580024790.01	-0.813645	2.304263e-51	1.513281e-48
MsG0280010182.01	MsG0580025135.01	0.806772	6.993569e-50	3.828514e-47
MsG0280010182.01	MsG0580025467.01	0.822533	2.250879e-53	1.889220e-50
MsG0280010182.01	MsG0580025587.01	0.800005	1.765508e-48	8.130717e-46
MsG0280010182.01	MsG0580025816.01	0.853916	1.741199e-61	3.822269e-58
MsG0280010182.01	MsG0580025987.01	0.825332	4.967378e-54	4.514157e-51
MsG0280010182.01	MsG0580026086.01	0.820000	8.644167e-53	6.757124e-50
MsG0280010182.01	MsG0580026131.01	0.815734	7.941104e-52	5.520021e-49
MsG0280010182.01	MsG0580026137.01	0.813527	2.446890e-51	1.601851e-48
MsG0280010182.01	MsG0580027361.01	0.830320	3.138232e-55	3.295010e-52
MsG0280010182.01	MsG0580027922.01	0.801614	8.284625e-49	3.973964e-46
MsG0280010182.01	MsG0580028044.01	0.826154	3.169013e-54	2.948027e-51
MsG0280010182.01	MsG0580028551.01	0.830343	3.097704e-55	3.254815e-52
MsG0280010182.01	MsG0580028582.01	0.830913	2.247085e-55	2.400922e-52
MsG0280010182.01	MsG0580028630.01	0.815109	1.093941e-51	7.474150e-49
MsG0280010182.01	MsG0580028754.01	0.852298	5.060641e-61	1.052820e-57
MsG0280010182.01	MsG0580029206.01	0.800578	1.349448e-48	6.305815e-46
MsG0280010182.01	MsG0580029236.01	0.822286	2.568859e-53	2.140955e-50
MsG0280010182.01	MsG0580029376.01	0.803260	3.794957e-49	1.898314e-46
MsG0280010182.01	MsG0580029549.01	0.844424	7.625814e-59	1.230592e-55
MsG0280010182.01	MsG0580029717.01	0.814550	1.455500e-51	9.795910e-49
MsG0280010182.01	MsG0580029879.01	0.854060	1.582969e-61	3.492267e-58
MsG0280010182.01	MsG0580029925.01	0.826786	2.240289e-54	2.121947e-51
MsG0280010182.01	MsG0580030142.01	0.832865	7.407545e-56	8.385722e-53
MsG0280010182.01	MsG0680030413.01	0.806492	8.011601e-50	4.354516e-47
MsG0280010182.01	MsG0680030415.01	0.812427	4.262206e-51	2.709359e-48
MsG0280010182.01	MsG0680030555.01	0.801638	8.191736e-49	3.931814e-46
MsG0280010182.01	MsG0680030616.01	0.809931	1.482756e-50	8.819953e-48
MsG0280010182.01	MsG0680030880.01	0.810641	1.042165e-50	6.316129e-48
MsG0280010182.01	MsG0680030992.01	0.806364	8.526041e-50	4.618880e-47
MsG0280010182.01	MsG0680031333.01	-0.803018	4.258082e-49	2.116734e-46
MsG0280010182.01	MsG0680032058.01	0.810090	1.370424e-50	8.185361e-48
MsG0280010182.01	MsG0680032204.01	0.812206	4.764846e-51	3.010695e-48
MsG0280010182.01	MsG0680032771.01	0.807287	5.441143e-50	3.018842e-47
MsG0280010182.01	MsG0680034230.01	0.843951	1.021256e-58	1.623887e-55
MsG0280010182.01	MsG0680034985.01	0.806983	6.309637e-50	3.473082e-47
MsG0280010182.01	MsG0680035877.01	0.818098	2.340337e-52	1.735383e-49
MsG0280010182.01	MsG0780035957.01	0.802009	6.875235e-49	3.331151e-46
MsG0280010182.01	MsG0780036183.01	0.801603	8.327582e-49	3.993366e-46
MsG0280010182.01	MsG0780036190.01	0.820796	5.676039e-53	4.535987e-50
MsG0280010182.01	MsG0780037082.01	0.809376	1.951459e-50	1.143892e-47
MsG0280010182.01	MsG0780039144.01	0.808772	2.628177e-50	1.515811e-47
MsG0280010182.01	MsG0780039170.01	0.815495	8.976303e-52	6.198804e-49
MsG0280010182.01	MsG0780039443.01	0.806115	9.619163e-50	5.178342e-47
MsG0280010182.01	MsG0780039522.01	0.846386	2.243961e-59	3.855951e-56
MsG0280010182.01	MsG0780040576.01	-0.803491	3.399117e-49	1.710486e-46
MsG0280010182.01	MsG0780040596.01	0.830207	3.344825e-55	3.500753e-52
MsG0280010182.01	MsG0780040734.01	0.814946	1.189201e-51	8.089223e-49
MsG0280010182.01	MsG0780040888.01	0.807565	4.751614e-50	2.655181e-47
MsG0280010182.01	MsG0780041137.01	0.819459	1.148828e-52	8.846141e-50
MsG0280010182.01	MsG0780041187.01	0.823716	1.192607e-53	1.035131e-50
MsG0280010182.01	MsG0780041387.01	0.807077	6.027255e-50	3.325605e-47
MsG0280010182.01	MsG0780041416.01	0.816665	4.918111e-52	3.506693e-49
MsG0280010182.01	MsG0780041435.01	0.824498	7.813502e-54	6.932680e-51
MsG0280010182.01	MsG0780041440.01	0.804420	2.179368e-49	1.123113e-46
MsG0280010182.01	MsG0780041441.01	0.804764	1.847025e-49	9.604081e-47
MsG0280010182.01	MsG0780041456.01	0.836409	9.516309e-57	1.198197e-53
MsG0280010182.01	MsG0780041475.01	0.814810	1.274267e-51	8.636642e-49
MsG0280010182.01	MsG0780041507.01	0.820353	7.175362e-53	5.664120e-50
MsG0280010182.01	MsG0880041861.01	0.810109	1.357536e-50	8.112922e-48
MsG0280010182.01	MsG0880041863.01	0.806641	7.454573e-50	4.066925e-47
MsG0280010182.01	MsG0880042031.01	0.802770	4.791975e-49	2.367361e-46
MsG0280010182.01	MsG0880042134.01	0.801094	1.058726e-48	5.012834e-46
MsG0280010182.01	MsG0880042324.01	0.814045	1.881767e-51	1.249251e-48
MsG0280010182.01	MsG0880042434.01	0.801381	9.251344e-49	4.411699e-46
MsG0280010182.01	MsG0880042622.01	0.809451	1.880012e-50	1.104173e-47
MsG0280010182.01	MsG0880042656.01	0.809386	1.941494e-50	1.138371e-47
MsG0280010182.01	MsG0880042700.01	0.829247	5.726885e-55	5.829218e-52
MsG0280010182.01	MsG0880043454.01	0.805277	1.442867e-49	7.601731e-47
MsG0280010182.01	MsG0880043509.01	0.803808	2.921258e-49	1.482008e-46
MsG0280010182.01	MsG0880044131.01	0.815155	1.068332e-51	7.308432e-49
MsG0280010182.01	MsG0880044728.01	0.824210	9.132160e-54	8.036325e-51
MsG0280010182.01	MsG0880044967.01	0.806160	9.413127e-50	5.073296e-47
MsG0280010182.01	MsG0880045017.01	0.804103	2.536464e-49	1.296615e-46
MsG0280010182.01	MsG0880045111.01	0.818701	1.708405e-52	1.288079e-49
MsG0280010182.01	MsG0880045362.01	0.813637	2.314186e-51	1.519432e-48
MsG0280010182.01	MsG0880045371.01	0.819829	9.458308e-53	7.358322e-50
MsG0280010182.01	MsG0880045387.01	0.802556	5.305220e-49	2.606462e-46
MsG0280010182.01	MsG0880045736.01	0.802991	4.313735e-49	2.142961e-46
MsG0280010182.01	MsG0880045765.01	0.843649	1.230531e-58	1.938304e-55
MsG0280010182.01	MsG0880045896.01	0.811521	6.714763e-51	4.165747e-48
MsG0280010182.01	MsG0880045992.01	0.808843	2.537441e-50	1.466267e-47
MsG0280010182.01	MsG0880046620.01	0.821618	3.669863e-53	3.000554e-50
MsG0280010182.01	MsG0880047052.01	0.807918	3.996420e-50	2.253880e-47
MsG0280010182.01	MsG0880047267.01	0.816136	6.459649e-52	4.539663e-49
MsG0280010182.01	MsG0880047763.01	0.810258	1.260690e-50	7.563300e-48
MsG0280010182.01	MsG0880047764.01	0.807113	5.923544e-50	3.271405e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010182.01.T01	MTR_2g079000	85.106	94	11	1	35	128	95	185	6.27e-46	149
MsG0280010182.01.T01	MTR_5g012820	54.491	167	29	3	9	128	109	275	3.23e-45	150
MsG0280010182.01.T01	MTR_5g012820	54.491	167	29	3	9	128	119	285	5.54e-45	150

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280010182.01.T01	AT5G05210	44.737	152	40	4	21	129	153	303	1.71e-26	102
MsG0280010182.01.T01	AT5G05210	44.737	152	40	4	21	129	161	311	1.81e-26	102
MsG0280010182.01.T01	AT2G27750	41.284	109	55	4	25	128	14	118	2.63e-15	68.9

Find 38 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGGTGAAGCTATTGCTAAAA+AGG	0.287342	2:-65840578	MsG0280010182.01.T01:CDS
GTATACTCCTTTATCAGTTT+AGG	0.307876	2:+65840502	None:intergenic
ATCGCAAACTTGAAGAGTTT+CGG	0.345780	2:-65840830	MsG0280010182.01.T01:intron
TGATGATCCTAAACTGATAA+AGG	0.379299	2:-65840509	MsG0280010182.01.T01:CDS
GCGGTTAAGCCTTTCATGTC+CGG	0.383891	2:-65840906	MsG0280010182.01.T01:CDS
GCAGCAATGAATGGAGCTTC+GGG	0.395137	2:-65840544	MsG0280010182.01.T01:CDS
TGAAAGAGCTAAGAAGTTAG+AGG	0.400614	2:-65840626	MsG0280010182.01.T01:CDS
CGGTTAAGCCTTTCATGTCC+GGG	0.416278	2:-65840905	MsG0280010182.01.T01:CDS
GCAATAGCTTCACCCTTCTC+AGG	0.419537	2:+65840586	None:intergenic
AGCAGCAATGAATGGAGCTT+CGG	0.423075	2:-65840545	MsG0280010182.01.T01:CDS
GTATACAGAAATGGAAGAAT+AGG	0.426673	2:-65840485	None:intergenic
TCTTTAAAAGAGCTTGTGTT+TGG	0.437000	2:-65840712	MsG0280010182.01.T01:CDS
ATGAAAGAAAGATAGAGTTC+CGG	0.443244	2:-65840764	MsG0280010182.01.T01:intron
TCCTTTACCACCTTCTCCTC+CGG	0.449534	2:+65840745	None:intergenic
GATAAAGGAGTATACAGAAA+TGG	0.456103	2:-65840494	MsG0280010182.01.T01:CDS
TCTTTCAAGTTCCTTGTGCT+TGG	0.476271	2:+65840642	None:intergenic
GCATGGAAAGCAGCAATGAA+TGG	0.491439	2:-65840553	MsG0280010182.01.T01:CDS
AGATAGAGTTCCGGAGGAGA+AGG	0.493563	2:-65840755	MsG0280010182.01.T01:intron
GAATGGAGCTTCGGGAATCA+AGG	0.503469	2:-65840536	MsG0280010182.01.T01:CDS
TCCGGAGGAGAAGGTGGTAA+AGG	0.513098	2:-65840746	MsG0280010182.01.T01:CDS
CCTTTCGCGTATTTCTTCAA+AGG	0.517323	2:+65840857	None:intergenic
CCGATCAGCTGCTTCAACCC+CGG	0.557235	2:+65840887	None:intergenic
GCTTCAACCCCGGACATGAA+AGG	0.559655	2:+65840897	None:intergenic
CCGGGGTTGAAGCAGCTGAT+CGG	0.569909	2:-65840887	MsG0280010182.01.T01:CDS
CTTCAGAACGGTGAAATGCA+AGG	0.570420	2:-65840679	MsG0280010182.01.T01:CDS
TAGAGTTCCGGAGGAGAAGG+TGG	0.580359	2:-65840752	MsG0280010182.01.T01:CDS
ATGAATGAACAGCGATGGAG+AGG	0.582050	2:-65840934	None:intergenic
GAAGAAGAATGATCCTGAGA+AGG	0.589096	2:-65840599	MsG0280010182.01.T01:CDS
ATGAACAGCGATGGAGAGGA+AGG	0.590401	2:-65840930	MsG0280010182.01.T01:CDS
AAGAAGAATGATCCTGAGAA+GGG	0.591026	2:-65840598	MsG0280010182.01.T01:CDS
AAAGAAAGATAGAGTTCCGG+AGG	0.593815	2:-65840761	MsG0280010182.01.T01:intron
GGCAATGTTAAACTTCAGAA+CGG	0.596133	2:-65840691	MsG0280010182.01.T01:CDS
TTCAGAACGGTGAAATGCAA+GGG	0.597324	2:-65840678	MsG0280010182.01.T01:CDS
GGTTAAGCCTTTCATGTCCG+GGG	0.644632	2:-65840904	MsG0280010182.01.T01:CDS
GGAGGAGAAGGTGGTAAAGG+AGG	0.646856	2:-65840743	MsG0280010182.01.T01:CDS
AGAGAAAGTTTCCAAGCACA+AGG	0.655350	2:-65840653	MsG0280010182.01.T01:CDS
CCTTTGAAGAAATACGCGAA+AGG	0.656007	2:-65840857	MsG0280010182.01.T01:CDS
CAGCGATGGAGAGGAAGGTG+CGG	0.679517	2:-65840925	MsG0280010182.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATCTGGAAAATGCTAATAAG+AGG	-	Chr2:65840628-65840647	MsG0280010182.01.T01:CDS	30.0%
	ATGAAAGAAAGATAGAGTTC+CGG	-	Chr2:65840652-65840671	MsG0280010182.01.T01:CDS	30.0%
	GTATACTCCTTTATCAGTTT+AGG	+	Chr2:65840917-65840936	None:intergenic	30.0%
	TCTGGAAAATGCTAATAAGA+GGG	-	Chr2:65840629-65840648	MsG0280010182.01.T01:CDS	30.0%
	TGATGATCCTAAACTGATAA+AGG	-	Chr2:65840907-65840926	MsG0280010182.01.T01:CDS	30.0%
!	GATAAAGGAGTATACAGAAA+TGG	-	Chr2:65840922-65840941	MsG0280010182.01.T01:CDS	30.0%
!	TCTTTAAAAGAGCTTGTGTT+TGG	-	Chr2:65840704-65840723	MsG0280010182.01.T01:CDS	30.0%
	AAGAAGAATGATCCTGAGAA+GGG	-	Chr2:65840818-65840837	MsG0280010182.01.T01:intron	35.0%
	ATCGCAAACTTGAAGAGTTT+CGG	-	Chr2:65840586-65840605	MsG0280010182.01.T01:CDS	35.0%
	CTATTGCTAAAAAGGAAGCA+TGG	-	Chr2:65840846-65840865	MsG0280010182.01.T01:CDS	35.0%
	GGCAATGTTAAACTTCAGAA+CGG	-	Chr2:65840725-65840744	MsG0280010182.01.T01:CDS	35.0%
	TGAAAGAGCTAAGAAGTTAG+AGG	-	Chr2:65840790-65840809	MsG0280010182.01.T01:intron	35.0%
!	TTTTCCAGATTTGCACAGTT+AGG	+	Chr2:65840618-65840637	None:intergenic	35.0%
!!	AAGCTCTTTTAAAGACTCTG+TGG	+	Chr2:65840699-65840718	None:intergenic	35.0%
	AAACTTGAAGAGTTTCGGTC+AGG	-	Chr2:65840591-65840610	MsG0280010182.01.T01:CDS	40.0%
	AAAGAAAGATAGAGTTCCGG+AGG	-	Chr2:65840655-65840674	MsG0280010182.01.T01:CDS	40.0%
	AGAGAAAGTTTCCAAGCACA+AGG	-	Chr2:65840763-65840782	MsG0280010182.01.T01:intron	40.0%
	CCTTTCGCGTATTTCTTCAA+AGG	+	Chr2:65840562-65840581	None:intergenic	40.0%
	GAAGAAGAATGATCCTGAGA+AGG	-	Chr2:65840817-65840836	MsG0280010182.01.T01:intron	40.0%
	GGGTGAAGCTATTGCTAAAA+AGG	-	Chr2:65840838-65840857	MsG0280010182.01.T01:CDS	40.0%
	TTCAGAACGGTGAAATGCAA+GGG	-	Chr2:65840738-65840757	MsG0280010182.01.T01:CDS	40.0%
!	CCTTTGAAGAAATACGCGAA+AGG	-	Chr2:65840559-65840578	MsG0280010182.01.T01:CDS	40.0%
!!	TCTTTCAAGTTCCTTGTGCT+TGG	+	Chr2:65840777-65840796	None:intergenic	40.0%
	AGCAGCAATGAATGGAGCTT+CGG	-	Chr2:65840871-65840890	MsG0280010182.01.T01:CDS	45.0%
	AGGTCCTAACTGTGCAAATC+TGG	-	Chr2:65840611-65840630	MsG0280010182.01.T01:CDS	45.0%
	CTTCAGAACGGTGAAATGCA+AGG	-	Chr2:65840737-65840756	MsG0280010182.01.T01:CDS	45.0%
	GCATGGAAAGCAGCAATGAA+TGG	-	Chr2:65840863-65840882	MsG0280010182.01.T01:CDS	45.0%
	AGATAGAGTTCCGGAGGAGA+AGG	-	Chr2:65840661-65840680	MsG0280010182.01.T01:CDS	50.0%
	ATGAACAGCGATGGAGAGGA+AGG	-	Chr2:65840486-65840505	MsG0280010182.01.T01:CDS	50.0%
	CGGTTAAGCCTTTCATGTCC+GGG	-	Chr2:65840511-65840530	MsG0280010182.01.T01:CDS	50.0%
	GAATGGAGCTTCGGGAATCA+AGG	-	Chr2:65840880-65840899	MsG0280010182.01.T01:CDS	50.0%
	GCAATAGCTTCACCCTTCTC+AGG	+	Chr2:65840833-65840852	None:intergenic	50.0%
	GCAGCAATGAATGGAGCTTC+GGG	-	Chr2:65840872-65840891	MsG0280010182.01.T01:CDS	50.0%
	GCGGTTAAGCCTTTCATGTC+CGG	-	Chr2:65840510-65840529	MsG0280010182.01.T01:CDS	50.0%
	GGTTAAGCCTTTCATGTCCG+GGG	-	Chr2:65840512-65840531	MsG0280010182.01.T01:CDS	50.0%
	TCCTTTACCACCTTCTCCTC+CGG	+	Chr2:65840674-65840693	None:intergenic	50.0%
	GCTTCAACCCCGGACATGAA+AGG	+	Chr2:65840522-65840541	None:intergenic	55.0%
	GGAGGAGAAGGTGGTAAAGG+AGG	-	Chr2:65840673-65840692	MsG0280010182.01.T01:CDS	55.0%
	TAGAGTTCCGGAGGAGAAGG+TGG	-	Chr2:65840664-65840683	MsG0280010182.01.T01:CDS	55.0%
	TCCGGAGGAGAAGGTGGTAA+AGG	-	Chr2:65840670-65840689	MsG0280010182.01.T01:CDS	55.0%
	CAGCGATGGAGAGGAAGGTG+CGG	-	Chr2:65840491-65840510	MsG0280010182.01.T01:CDS	60.0%
	CCGATCAGCTGCTTCAACCC+CGG	+	Chr2:65840532-65840551	None:intergenic	60.0%
	CCGGGGTTGAAGCAGCTGAT+CGG	-	Chr2:65840529-65840548	MsG0280010182.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	65840486	65840952	65840486	ID=MsG0280010182.01;Name=MsG0280010182.01
Chr2	mRNA	65840486	65840952	65840486	ID=MsG0280010182.01.T01;Parent=MsG0280010182.01;Name=MsG0280010182.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|1\|1\|2\|0\|135
Chr2	exon	65840831	65840952	65840831	ID=MsG0280010182.01.T01:exon:18187;Parent=MsG0280010182.01.T01
Chr2	exon	65840486	65840771	65840486	ID=MsG0280010182.01.T01:exon:18186;Parent=MsG0280010182.01.T01
Chr2	CDS	65840831	65840952	65840831	ID=MsG0280010182.01.T01:cds;Parent=MsG0280010182.01.T01
Chr2	CDS	65840486	65840771	65840486	ID=MsG0280010182.01.T01:cds;Parent=MsG0280010182.01.T01

Gene Sequence

>MsG0280010182.01.T01

ATGAACAGCGATGGAGAGGAAGGTGCGGTTAAGCCTTTCATGTCCGGGGTTGAAGCAGCTGATCGGTTTGCGACCTTTGAAGAAATACGCGAAAGGCTTCATCGCAAACTTGAAGAGTTTCGAGTTCCGGAGGAGAAGGTGGTAAAGGAGGCCACAGAGTCTTTAAAAGAGCTTGTGTTTGGCAATGTTAAACTTCAGAACGGTGAAATGCAAGGGAAAGAGAAAGTTTCCAAGCACAAGGAACTTGAAAGAGCTAAGAAGTTAGAGGAAGTGAAGAAGAATGATCCTGAGAAGGGTGAAGCTATTGCTAAAAAGGAAGCATGGAAAGCAGCAATGAATGGAGCTTCGGGAATCAAGGTTCATGATGATCCTAAACTGATAAAGGAGTATACAGAAATGGAAGAATAG

Protein sequence

>MsG0280010182.01.T01

MNSDGEEGAVKPFMSGVEAADRFATFEEIRERLHRKLEEFRVPEEKVVKEATESLKELVFGNVKLQNGEMQGKEKVSKHKELERAKKLEEVKKNDPEKGEAIAKKEAWKAAMNGASGIKVHDDPKLIKEYTEMEE*