AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022986.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022986.01.T01 MTR_8g080860 78.000 100 22 0 1 100 1 100 1.48e-47 154
MsG0480022986.01.T01 MTR_8g080860 75.385 65 13 1 43 107 135 196 3.90e-23 90.9
MsG0480022986.01.T01 MTR_8g080860 61.667 60 23 0 43 102 181 240 1.35e-16 73.2
MsG0480022986.01.T01 MTR_8g080860 77.083 96 22 0 5 100 1 96 1.90e-44 145
MsG0480022986.01.T01 MTR_8g080860 75.385 65 13 1 43 107 131 192 4.43e-23 90.5
MsG0480022986.01.T01 MTR_8g080860 61.667 60 23 0 43 102 177 236 1.63e-16 73.2
MsG0480022986.01.T01 MTR_8g080340 51.200 125 37 2 1 102 1 124 5.20e-31 108
MsG0480022986.01.T01 MTR_8g088930 43.956 91 51 0 8 98 1 91 3.76e-20 84.3
MsG0480022986.01.T01 MTR_8g088930 42.857 84 48 0 17 100 102 185 7.21e-17 75.1
MsG0480022986.01.T01 MTR_8g088930 43.956 91 51 0 8 98 1 91 3.87e-20 84.3
MsG0480022986.01.T01 MTR_8g088930 42.857 84 48 0 17 100 102 185 7.43e-17 75.1
MsG0480022986.01.T01 MTR_8g088930 43.478 92 52 0 8 99 1 92 4.06e-20 84.3
MsG0480022986.01.T01 MTR_8g088930 41.379 87 51 0 14 100 99 185 6.80e-17 75.1
MsG0480022986.01.T01 MTR_8g088930 43.478 92 52 0 8 99 1 92 4.18e-20 84.3
MsG0480022986.01.T01 MTR_8g088930 41.379 87 51 0 14 100 99 185 6.86e-17 75.1
MsG0480022986.01.T01 MTR_8g080310 63.793 58 21 0 43 100 33 90 9.93e-19 76.6
MsG0480022986.01.T01 MTR_8g080310 58.621 58 24 0 43 100 79 136 6.51e-16 69.3
MsG0480022986.01.T01 MTR_8g080310 67.391 46 15 0 57 102 1 46 1.39e-14 65.9
MsG0480022986.01.T01 MTR_8g092410 51.282 78 33 2 24 99 115 189 1.76e-17 76.6
MsG0480022986.01.T01 MTR_8g092410 45.349 86 36 3 21 99 180 261 2.36e-12 62.0
MsG0480022986.01.T01 MTR_8g080350 41.837 98 52 3 4 99 42 136 4.55e-17 75.5
MsG0480022986.01.T01 MTR_8g080350 52.830 53 25 0 55 107 291 343 7.72e-12 60.5
MsG0480022986.01.T01 MTR_8g092090 44.318 88 47 1 12 99 34 119 7.73e-16 70.9
MsG0480022986.01.T01 MTR_8g092090 56.897 58 25 0 43 100 109 166 5.67e-15 68.6
MsG0480022986.01.T01 MTR_8g092090 53.448 58 27 0 42 99 154 211 3.73e-13 63.5
MsG0480022986.01.T01 MTR_6g043150 43.038 79 45 0 21 99 80 158 2.44e-14 67.4
MsG0480022986.01.T01 MTR_6g043150 38.372 86 53 0 14 99 4 89 4.40e-11 58.5
MsG0480022986.01.T01 MTR_2g078470 41.860 86 50 0 14 99 4 89 6.64e-14 66.6
MsG0480022986.01.T01 MTR_2g078470 33.036 112 62 1 1 99 254 365 5.69e-12 60.8
MsG0480022986.01.T01 MTR_2g078470 35.366 82 53 0 18 99 215 296 1.10e-11 60.1
MsG0480022986.01.T01 MTR_2g078470 37.975 79 49 0 21 99 80 158 3.18e-11 58.9
MsG0480022986.01.T01 MTR_8g095240 43.617 94 43 2 19 102 118 211 9.45e-14 66.2
MsG0480022986.01.T01 MTR_8g095240 65.909 44 15 0 59 102 215 258 1.28e-13 65.9
MsG0480022986.01.T01 MTR_8g095240 56.897 58 25 0 42 99 244 301 1.85e-13 65.1
MsG0480022986.01.T01 MTR_8g095240 50.000 60 30 0 39 98 69 128 7.54e-13 63.5
MsG0480022986.01.T01 MTR_5g058920 55.102 49 22 0 50 98 227 275 5.36e-13 63.9
MsG0480022986.01.T01 MTR_3g023310 52.542 59 28 0 44 102 53 111 1.25e-12 61.2
MsG0480022986.01.T01 MTR_4g107460 54.717 53 24 0 50 102 132 184 2.28e-12 62.0
MsG0480022986.01.T01 MTR_4g107460 35.849 106 54 1 11 102 33 138 4.16e-12 61.2
MsG0480022986.01.T01 MTR_4g107460 50.000 58 29 0 42 99 170 227 5.12e-12 61.2
MsG0480022986.01.T01 MTR_3g097260 39.759 83 50 0 16 98 143 225 2.32e-12 62.4
MsG0480022986.01.T01 MTR_5g459450 34.783 92 60 0 7 98 56 147 1.09e-11 60.5
MsG0480022986.01.T01 MTR_7g451440 38.750 80 49 0 19 98 191 270 2.19e-11 59.3
MsG0480022986.01.T01 MTR_5g010840 37.975 79 49 0 21 99 266 344 2.98e-11 58.9
MsG0480022986.01.T01 MTR_3g014650 36.905 84 53 0 17 100 166 249 6.69e-11 58.2
MsG0480022986.01.T01 MTR_7g089950 36.471 85 54 0 14 98 65 149 7.76e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022986.01.T01 AT1G60890 44.048 84 47 0 17 100 100 183 1.29e-16 74.3
MsG0480022986.01.T01 AT1G60890 37.349 83 52 0 16 98 7 89 1.19e-13 65.9
MsG0480022986.01.T01 AT1G60890 43.529 85 48 0 16 100 111 195 1.29e-16 74.3
MsG0480022986.01.T01 AT1G60890 36.905 84 53 0 16 99 19 102 1.34e-13 65.9
MsG0480022986.01.T01 AT1G10900 47.458 59 31 0 42 100 125 183 5.12e-14 67.0
MsG0480022986.01.T01 AT1G10900 36.145 83 53 0 16 98 7 89 6.79e-12 60.8
MsG0480022986.01.T01 AT1G10900 47.458 59 31 0 42 100 125 183 5.12e-14 67.0
MsG0480022986.01.T01 AT1G10900 36.145 83 53 0 16 98 7 89 6.79e-12 60.8
MsG0480022986.01.T01 AT1G10900 47.458 59 31 0 42 100 125 183 5.12e-14 67.0
MsG0480022986.01.T01 AT1G10900 36.145 83 53 0 16 98 7 89 6.79e-12 60.8
MsG0480022986.01.T01 AT1G21980 36.145 83 53 0 16 98 72 154 2.94e-12 62.0
MsG0480022986.01.T01 AT1G77740 33.333 93 62 0 16 108 165 257 8.74e-12 60.5
MsG0480022986.01.T01 AT1G77740 33.333 93 62 0 16 108 165 257 8.74e-12 60.5
MsG0480022986.01.T01 AT2G41210 36.905 84 53 0 17 100 159 242 2.29e-11 59.3
MsG0480022986.01.T01 AT3G07960 37.349 83 52 0 16 98 23 105 3.51e-11 58.9
MsG0480022986.01.T01 AT3G07960 37.349 83 52 0 16 98 23 105 3.51e-11 58.9
MsG0480022986.01.T01 AT3G07960 37.349 83 52 0 16 98 23 105 3.60e-11 58.9
MsG0480022986.01.T01 AT3G07960 37.349 83 52 0 16 98 23 105 3.62e-11 58.9
MsG0480022986.01.T01 AT4G17080 32.927 82 55 0 17 98 254 335 3.68e-11 58.9
MsG0480022986.01.T01 AT3G56960 35.366 82 53 0 17 98 161 242 5.66e-11 58.2
MsG0480022986.01.T01 AT3G56960 35.366 82 53 0 17 98 161 242 5.66e-11 58.2

Find 40 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACGTCTACACTGGAAAT+TGG 0.199973 4:-79446271 MsG0480022986.01.T01:CDS
TGACCAACAACCATCGAAAA+TGG 0.303459 4:+79446337 None:intergenic
AGGGAAAGGGATTATGAATT+GGG 0.375715 4:-79446369 MsG0480022986.01.T01:CDS
CAGGGAAAGGGATTATGAAT+TGG 0.387625 4:-79446370 MsG0480022986.01.T01:CDS
TTCGATGGTTGTTGGTCAAA+TGG 0.393393 4:-79446332 MsG0480022986.01.T01:CDS
AATGGACTTTCACATGGATA+TGG 0.393948 4:-79446314 MsG0480022986.01.T01:CDS
CATGGAAAGGGATTATGAAA+TGG 0.430623 4:-79446234 MsG0480022986.01.T01:CDS
ATACGAGGTGACTGGGTTGA+TGG 0.431562 4:-79446401 MsG0480022986.01.T01:CDS
AAACGTCTACACTGGAAATT+GGG 0.451480 4:-79446270 MsG0480022986.01.T01:CDS
GGGATTATGAATTGGGGTAA+TGG 0.470324 4:-79446362 MsG0480022986.01.T01:CDS
ATGTCTACATGGTAAATTCA+AGG 0.477466 4:-79446475 MsG0480022986.01.T01:CDS
TCAAAATGGGGATGTCTACA+TGG 0.484109 4:-79446486 MsG0480022986.01.T01:CDS
GAAAGTACACCATGGACAAA+TGG 0.488701 4:-79446431 MsG0480022986.01.T01:CDS
ATGGAAAGGGATTATGAAAT+GGG 0.490705 4:-79446233 MsG0480022986.01.T01:CDS
GAACTAATATACGAGGTGAC+TGG 0.494367 4:-79446409 MsG0480022986.01.T01:CDS
GATGGAAAAGTGACAGGGAA+AGG 0.496612 4:-79446383 MsG0480022986.01.T01:CDS
ATGCAAGTCATGCAGGATGA+TGG 0.511935 4:-79446661 MsG0480022986.01.T01:CDS
GGAGTTTATAGATCTGCAAA+TGG 0.513797 4:-79446293 MsG0480022986.01.T01:CDS
AAAGGTGAAAGGCATGGAAA+GGG 0.515947 4:-79446246 MsG0480022986.01.T01:CDS
ATATTAGTTCCATTTGTCCA+TGG 0.518713 4:+79446422 None:intergenic
ATGGAAAAGTGACAGGGAAA+GGG 0.534561 4:-79446382 MsG0480022986.01.T01:CDS
CTACACTGGAAATTGGGAAA+AGG 0.534830 4:-79446264 MsG0480022986.01.T01:CDS
AAAAGGTGAAAGGCATGGAA+AGG 0.537435 4:-79446247 MsG0480022986.01.T01:CDS
AACTAATATACGAGGTGACT+GGG 0.539684 4:-79446408 MsG0480022986.01.T01:CDS
TGGGTTGATGGAAAAGTGAC+AGG 0.540161 4:-79446389 MsG0480022986.01.T01:CDS
TGGTGTACTTTCCGATGCCA+TGG 0.566918 4:+79446442 None:intergenic
TGAAAGAATGCAAGTCATGC+AGG 0.574263 4:-79446668 MsG0480022986.01.T01:CDS
TGGTCAAATGGACTTTCACA+TGG 0.576790 4:-79446320 MsG0480022986.01.T01:CDS
TTGGGAAAAGGTGAAAGGCA+TGG 0.577872 4:-79446252 MsG0480022986.01.T01:CDS
GGTGAACTTCTCCATGGCAT+CGG 0.587755 4:-79446453 MsG0480022986.01.T01:CDS
GCAAATGGAAACGTCTACAC+TGG 0.588126 4:-79446278 MsG0480022986.01.T01:CDS
TGTCTACATGGTAAATTCAA+GGG 0.597621 4:-79446474 MsG0480022986.01.T01:CDS
GGAAATTGGGAAAAGGTGAA+AGG 0.603777 4:-79446257 MsG0480022986.01.T01:CDS
TTCAAGGGTGAACTTCTCCA+TGG 0.633264 4:-79446459 MsG0480022986.01.T01:CDS
GGGATTATGAAATGGGCTAA+CGG 0.641974 4:-79446226 None:intergenic
AAGTCATGCAGGATGATGGA+AGG 0.643845 4:-79446657 MsG0480022986.01.T01:intron
GGGAAAGGGATTATGAATTG+GGG 0.649116 4:-79446368 MsG0480022986.01.T01:CDS
GGGTTGATGGAAAAGTGACA+GGG 0.720292 4:-79446388 MsG0480022986.01.T01:CDS
TGGCATCGGAAAGTACACCA+TGG 0.731921 4:-79446439 MsG0480022986.01.T01:CDS
ACAAATGGAACTAATATACG+AGG 0.756102 4:-79446416 MsG0480022986.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAATCATAATCATAATAAA+TGG + Chr4:79446357-79446376 None:intergenic 15.0%
!! AAAGAGAAGTCTTTTCAAAA+TGG - Chr4:79446402-79446421 MsG0480022986.01.T01:CDS 25.0%
!! AAGAGAAGTCTTTTCAAAAT+GGG - Chr4:79446403-79446422 MsG0480022986.01.T01:CDS 25.0%
ACAAATGGAACTAATATACG+AGG - Chr4:79446486-79446505 MsG0480022986.01.T01:CDS 30.0%
ATATTAGTTCCATTTGTCCA+TGG + Chr4:79446483-79446502 None:intergenic 30.0%
ATGGAAAGGGATTATGAAAT+GGG - Chr4:79446669-79446688 MsG0480022986.01.T01:CDS 30.0%
ATGTCTACATGGTAAATTCA+AGG - Chr4:79446427-79446446 MsG0480022986.01.T01:CDS 30.0%
TGAATTATATAGAGTAGCTG+TGG + Chr4:79446286-79446305 None:intergenic 30.0%
TGTCTACATGGTAAATTCAA+GGG - Chr4:79446428-79446447 MsG0480022986.01.T01:CDS 30.0%
! AGAGAAGTCTTTTCAAAATG+GGG - Chr4:79446404-79446423 MsG0480022986.01.T01:CDS 30.0%
AAACGTCTACACTGGAAATT+GGG - Chr4:79446632-79446651 MsG0480022986.01.T01:intron 35.0%
AACTAATATACGAGGTGACT+GGG - Chr4:79446494-79446513 MsG0480022986.01.T01:CDS 35.0%
AGGGAAAGGGATTATGAATT+GGG - Chr4:79446533-79446552 MsG0480022986.01.T01:intron 35.0%
CATGGAAAGGGATTATGAAA+TGG - Chr4:79446668-79446687 MsG0480022986.01.T01:CDS 35.0%
GGAGTTTATAGATCTGCAAA+TGG - Chr4:79446609-79446628 MsG0480022986.01.T01:intron 35.0%
! AATGGACTTTCACATGGATA+TGG - Chr4:79446588-79446607 MsG0480022986.01.T01:intron 35.0%
ATGGAAAAGTGACAGGGAAA+GGG - Chr4:79446520-79446539 MsG0480022986.01.T01:intron 40.0%
CAGGGAAAGGGATTATGAAT+TGG - Chr4:79446532-79446551 MsG0480022986.01.T01:intron 40.0%
CTACACTGGAAATTGGGAAA+AGG - Chr4:79446638-79446657 MsG0480022986.01.T01:intron 40.0%
GAAACGTCTACACTGGAAAT+TGG - Chr4:79446631-79446650 MsG0480022986.01.T01:intron 40.0%
GAACTAATATACGAGGTGAC+TGG - Chr4:79446493-79446512 MsG0480022986.01.T01:CDS 40.0%
GGAAATTGGGAAAAGGTGAA+AGG - Chr4:79446645-79446664 MsG0480022986.01.T01:intron 40.0%
GGGAAAGGGATTATGAATTG+GGG - Chr4:79446534-79446553 MsG0480022986.01.T01:intron 40.0%
GGGATTATGAATTGGGGTAA+TGG - Chr4:79446540-79446559 MsG0480022986.01.T01:intron 40.0%
TCAAAATGGGGATGTCTACA+TGG - Chr4:79446416-79446435 MsG0480022986.01.T01:CDS 40.0%
TGAAAGAATGCAAGTCATGC+AGG - Chr4:79446234-79446253 MsG0480022986.01.T01:CDS 40.0%
TGACCAACAACCATCGAAAA+TGG + Chr4:79446568-79446587 None:intergenic 40.0%
TTCGATGGTTGTTGGTCAAA+TGG - Chr4:79446570-79446589 MsG0480022986.01.T01:intron 40.0%
! AAAAGGTGAAAGGCATGGAA+AGG - Chr4:79446655-79446674 MsG0480022986.01.T01:intron 40.0%
! AAAGGTGAAAGGCATGGAAA+GGG - Chr4:79446656-79446675 MsG0480022986.01.T01:intron 40.0%
! GAAAGTACACCATGGACAAA+TGG - Chr4:79446471-79446490 MsG0480022986.01.T01:CDS 40.0%
! TGGTCAAATGGACTTTCACA+TGG - Chr4:79446582-79446601 MsG0480022986.01.T01:intron 40.0%
ATGCAAGTCATGCAGGATGA+TGG - Chr4:79446241-79446260 MsG0480022986.01.T01:CDS 45.0%
GATGGAAAAGTGACAGGGAA+AGG - Chr4:79446519-79446538 MsG0480022986.01.T01:intron 45.0%
GCAAATGGAAACGTCTACAC+TGG - Chr4:79446624-79446643 MsG0480022986.01.T01:intron 45.0%
TTCAAGGGTGAACTTCTCCA+TGG - Chr4:79446443-79446462 MsG0480022986.01.T01:CDS 45.0%
TTGGGAAAAGGTGAAAGGCA+TGG - Chr4:79446650-79446669 MsG0480022986.01.T01:intron 45.0%
!! AAGTCATGCAGGATGATGGA+AGG - Chr4:79446245-79446264 MsG0480022986.01.T01:CDS 45.0%
!! GGGTTGATGGAAAAGTGACA+GGG - Chr4:79446514-79446533 MsG0480022986.01.T01:intron 45.0%
!! TGGGTTGATGGAAAAGTGAC+AGG - Chr4:79446513-79446532 MsG0480022986.01.T01:intron 45.0%
!!! GGTAATGGTGCCATTTTCGA+TGG - Chr4:79446555-79446574 MsG0480022986.01.T01:intron 45.0%
!!! GTGCCATTTTCGATGGTTGT+TGG - Chr4:79446562-79446581 MsG0480022986.01.T01:intron 45.0%
GGTGAACTTCTCCATGGCAT+CGG - Chr4:79446449-79446468 MsG0480022986.01.T01:CDS 50.0%
TGGTGTACTTTCCGATGCCA+TGG + Chr4:79446463-79446482 None:intergenic 50.0%
! ATACGAGGTGACTGGGTTGA+TGG - Chr4:79446501-79446520 MsG0480022986.01.T01:CDS 50.0%
! TGGCATCGGAAAGTACACCA+TGG - Chr4:79446463-79446482 MsG0480022986.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 79446229 79446695 79446229 ID=MsG0480022986.01;Name=MsG0480022986.01
Chr4 mRNA 79446229 79446695 79446229 ID=MsG0480022986.01.T01;Parent=MsG0480022986.01;Name=MsG0480022986.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|0|0|2|0|111
Chr4 exon 79446658 79446695 79446658 ID=MsG0480022986.01.T01:exon:3998;Parent=MsG0480022986.01.T01
Chr4 exon 79446229 79446526 79446229 ID=MsG0480022986.01.T01:exon:3997;Parent=MsG0480022986.01.T01
Chr4 CDS 79446658 79446695 79446658 ID=MsG0480022986.01.T01:cds;Parent=MsG0480022986.01.T01
Chr4 CDS 79446229 79446526 79446229 ID=MsG0480022986.01.T01:cds;Parent=MsG0480022986.01.T01
Gene Sequence

>MsG0480022986.01.T01

ATGAGTGAAAGAATGCAAGTCATGCAGGATGATGGAAGTGTTAAAGAGAAGTCTTTTCAAAATGGGGATGTCTACATGGTAAATTCAAGGGTGAACTTCTCCATGGCATCGGAAAGTACACCATGGACAAATGGAACTAATATACGAGGTGACTGGGTTGATGGAAAAGTGACAGGGAAAGGGATTATGAATTGGGGTAATGGTGCCATTTTCGATGGTTGTTGGTCAAATGGACTTTCACATGGATATGGAGTTTATAGATCTGCAAATGGAAACGTCTACACTGGAAATTGGGAAAAGGTGAAAGGCATGGAAAGGGATTATGAAATGGGCTAA

Protein sequence

>MsG0480022986.01.T01

MSERMQVMQDDGSVKEKSFQNGDVYMVNSRVNFSMASESTPWTNGTNIRGDWVDGKVTGKGIMNWGNGAIFDGCWSNGLSHGYGVYRSANGNVYTGNWEKVKGMERDYEMG*