AlfalfaGEDB | Msa0011250

M. sativa cultivar zhongmu-4 / Msa0011250

KEGG: K00128
GO: GO:0001101 GO:0003674 GO:0003824 GO:0004028 GO:0004029 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005794 GO:0005911 GO:0006950 GO:0006970 GO:0008150 GO:0008152 GO:0009269 GO:0009414 GO:0009415 GO:0009506 GO:0009536 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0010035 GO:0012505 GO:0016020 GO:0016491 GO:0016620 GO:0016903 GO:0030054 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0055044 GO:0055114 GO:0097305 GO:1901700
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0011250	RHN77911.1	96.250	80	3	0	1	80	1	80	3.13e-46	154

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0011250	sp\|Q70DU8\|AL3H1_ARATH	56.522	69	30	0	8	76	4	72	1.33e-17	78.6

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0011250	A0A396JML5	96.250	80	3	0	1	80	1	80	1.49e-46	154

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0011250	Msa0026910	0.816378	5.702562e-52	-8.615850e-47
Msa0011250	Msa0064110	0.808957	2.399494e-50	-8.615850e-47
Msa0011250	Msa0077450	0.802322	5.928376e-49	-8.615850e-47
Msa0011250	Msa0078060	0.842131	3.118198e-58	-8.615850e-47
Msa0011250	Msa0105070	0.818065	2.381214e-52	-8.615850e-47
Msa0011250	Msa0122580	0.814324	1.632490e-51	-8.615850e-47
Msa0011250	Msa0123300	0.818706	1.703999e-52	-8.615850e-47
Msa0011250	Msa0166870	0.803124	4.048562e-49	-8.615850e-47
Msa0011250	Msa0170140	0.804789	1.824567e-49	-8.615850e-47
Msa0011250	Msa0223410	-0.803221	3.865871e-49	-8.615850e-47
Msa0011250	Msa0237310	0.816459	5.470984e-52	-8.615850e-47
Msa0011250	Msa0249100	0.815465	9.117958e-52	-8.615850e-47
Msa0011250	Msa0274730	0.818118	2.316284e-52	-8.615850e-47
Msa0011250	Msa0308460	0.805144	1.538144e-49	-8.615850e-47
Msa0011250	Msa0315530	0.806448	8.184628e-50	-8.615850e-47
Msa0011250	Msa0317740	0.807497	4.910344e-50	-8.615850e-47
Msa0011250	Msa0340690	0.802516	5.405963e-49	-8.615850e-47
Msa0011250	Msa0365490	0.808106	3.645876e-50	-8.615850e-47
Msa0011250	Msa0376590	0.805401	1.359129e-49	-8.615850e-47
Msa0011250	Msa0382870	0.802855	4.601995e-49	-8.615850e-47
Msa0011250	Msa0387250	0.804858	1.765479e-49	-8.615850e-47
Msa0011250	Msa0409200	0.801296	9.626801e-49	-8.615850e-47
Msa0011250	Msa0432930	0.800584	1.346049e-48	-8.615850e-47
Msa0011250	Msa0436370	0.816813	4.557345e-52	-8.615850e-47
Msa0011250	Msa0439970	0.816066	6.696964e-52	-8.615850e-47
Msa0011250	Msa0458440	0.812443	4.228202e-51	-8.615850e-47
Msa0011250	Msa0559500	0.801015	1.099059e-48	-8.615850e-47
Msa0011250	Msa0561910	0.802811	4.699394e-49	-8.615850e-47
Msa0011250	Msa0572480	0.809954	1.465488e-50	-8.615850e-47
Msa0011250	Msa0626280	0.809529	1.808698e-50	-8.615850e-47
Msa0011250	Msa0626850	0.805909	1.063236e-49	-8.615850e-47
Msa0011250	Msa0685750	0.802957	4.384855e-49	-8.615850e-47
Msa0011250	Msa0688070	0.815133	1.080596e-51	-8.615850e-47
Msa0011250	Msa0693380	0.805148	1.535611e-49	-8.615850e-47
Msa0011250	Msa0711130	0.800508	1.394521e-48	-8.615850e-47
Msa0011250	Msa0760130	0.821000	5.095512e-53	-8.615850e-47
Msa0011250	Msa0798860	0.801761	7.730017e-49	-8.615850e-47
Msa0011250	Msa0828540	0.809770	1.605617e-50	-8.615850e-47
Msa0011250	Msa0828660	0.817565	3.086709e-52	-8.615850e-47
Msa0011250	Msa0832090	0.800530	1.380131e-48	-8.615850e-47
Msa0011250	Msa0832320	0.808961	2.394257e-50	-8.615850e-47
Msa0011250	Msa0924310	0.806820	6.833394e-50	-8.615850e-47
Msa0011250	Msa0969520	0.802993	4.310271e-49	-8.615850e-47
Msa0011250	Msa1000880	0.801028	1.092299e-48	-8.615850e-47
Msa0011250	Msa1039710	-0.803189	3.925845e-49	-8.615850e-47
Msa0011250	Msa1052330	0.811391	7.168764e-51	-8.615850e-47
Msa0011250	Msa1068200	-0.813854	2.072838e-51	-8.615850e-47
Msa0011250	Msa1081330	0.816613	5.051836e-52	-8.615850e-47
Msa0011250	Msa1179970	0.800031	1.744310e-48	-8.615850e-47
Msa0011250	Msa1224480	0.810740	9.916574e-51	-8.615850e-47
Msa0011250	Msa1229600	0.831853	1.319216e-55	-8.615850e-47
Msa0011250	Msa1276440	0.804679	1.923899e-49	-8.615850e-47
Msa0011250	Msa1305350	0.806032	1.001508e-49	-8.615850e-47
Msa0011250	Msa1310210	0.815090	1.104656e-51	-8.615850e-47
Msa0011250	Msa1332000	0.820457	6.790817e-53	-8.615850e-47
Msa0011250	Msa1333680	0.800233	1.586370e-48	-8.615850e-47
Msa0011250	Msa1333730	0.833811	4.304623e-56	-8.615850e-47
Msa0011250	Msa1372760	0.822847	1.902999e-53	-8.615850e-47
Msa0011250	Msa1379850	0.820207	7.749736e-53	-8.615850e-47
Msa0011250	Msa1393410	0.831585	1.535438e-55	-8.615850e-47
Msa0011250	Msa1424680	0.800070	1.712434e-48	-8.615850e-47
Msa0011250	Msa1435420	0.830953	2.195816e-55	-8.615850e-47
Msa0011250	Msa1451620	-0.807230	5.594805e-50	-8.615850e-47
Msa0011250	Msa1462930	0.811950	5.416198e-51	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0011250	MtrunA17_Chr1g0159821	96.250	80	3	1	80	1	80	2.88e-50	154
Msa0011250	MtrunA17_Chr3g0124811	51.389	72	35	8	79	10	81	1.32e-20	84.0
Msa0011250	MtrunA17_Chr8g0384741	48.571	70	36	8	77	61	130	2.90e-19	80.1
Msa0011250	MtrunA17_Chr1g0159811	52.174	69	33	8	76	8	76	3.79e-17	73.9
Msa0011250	MtrunA17_Chr8g0372381	48.387	62	32	15	76	8	69	7.23e-13	62.0

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0011250	AT1G44170.1	56.522	69	30	8	76	4	72	1.36e-18	78.6
Msa0011250	AT1G44170.2	56.522	69	30	8	76	4	72	1.36e-18	78.6
Msa0011250	AT4G34240.2	48.529	68	35	11	78	70	137	9.68e-17	73.2
Msa0011250	AT4G34240.4	48.529	68	35	11	78	55	122	1.24e-16	73.2
Msa0011250	AT4G34240.1	48.529	68	35	11	78	70	137	1.25e-16	73.2

Find 7 sgRNAs with CRISPR-Local

Find 9 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTCAGTTGAAGGCACTTC+TGG	0.189731	1_1:-16981039	Msa0011250:CDS
TTGAGAATAACGTTTGATTC+TGG	0.302281	1_1:-16981089	Msa0011250:CDS
ATGGAAAGTTTCTCAGTTGA+AGG	0.449116	1_1:-16981048	Msa0011250:CDS
ATGAGAAATTGAGTACCTCT+TGG	0.488000	1_1:+16980937	None:intergenic
ATTGAGTACCTCTTGGATGA+AGG	0.488247	1_1:+16980944	None:intergenic
GTAAGACTCGAAGCTACGAA+TGG	0.580175	1_1:-16981067	Msa0011250:CDS
AGCTGAATCCTTCATCCAAG+AGG	0.631889	1_1:-16980952	Msa0011250:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTTTTTGTTGAGTTTAGAAG+AGG	+	chr1_1:16980942-16980961	None:intergenic	25.0%
!!!	CAACAAAAAAGTGTTTGATT+TGG	-	chr1_1:16980952-16980971	Msa0011250:CDS	25.0%
	TTGAGAATAACGTTTGATTC+TGG	-	chr1_1:16981001-16981020	Msa0011250:CDS	30.0%
	ATGGAAAGTTTCTCAGTTGA+AGG	-	chr1_1:16981042-16981061	Msa0011250:CDS	35.0%
	ATGAGAAATTGAGTACCTCT+TGG	+	chr1_1:16981156-16981175	None:intergenic	35.0%
	ATTGAGTACCTCTTGGATGA+AGG	+	chr1_1:16981149-16981168	None:intergenic	40.0%
	GTAAGACTCGAAGCTACGAA+TGG	-	chr1_1:16981023-16981042	Msa0011250:CDS	45.0%
!!	TTCTCAGTTGAAGGCACTTC+TGG	-	chr1_1:16981051-16981070	Msa0011250:CDS	45.0%
	AGCTGAATCCTTCATCCAAG+AGG	-	chr1_1:16981138-16981157	Msa0011250:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr1_1	gene	16980935	16981177	16980935	ID=Msa0011250;Name=Msa0011250
chr1_1	mRNA	16980935	16981177	16980935	ID=Msa0011250-mRNA-1;Parent=Msa0011250;Name=Msa0011250-mRNA-1;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|80
chr1_1	exon	16980935	16981177	16980935	ID=Msa0011250-mRNA-1:exon:6206;Parent=Msa0011250-mRNA-1
chr1_1	CDS	16980935	16981177	16980935	ID=Msa0011250-mRNA-1:cds;Parent=Msa0011250-mRNA-1

Gene Sequence	>Msa0011250 ATGGCCTCTTCTAAACTCAACAAAAAAGTGTTTGATTTGGAAGAAGCTTCAACAACAGTTAAAGATTTGAGAATAACGTTTGATTCTGGTAAGACTCGAAGCTACGAATGGAAAGTTTCTCAGTTGAAGGCACTTCTGGAATTAACTGATAAGCATGAGCAAGAGATTGTTGAAGCTCTTTACTCTGACTTATCAAAATCTGAAGCTGAATCCTTCATCCAAGAGGTACTCAATTTCTCATAG
Protein sequence	>Msa0011250 MASSKLNKKVFDLEEASTTVKDLRITFDSGKTRSYEWKVSQLKALLELTDKHEQEIVEALYSDLSKSEAESFIQEVLNFS*