AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0012700


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0012700 MtrunA17_Chr8g0359031 46.364 110 58 1 11 120 38 146 9.19e-34 115
Msa0012700 MtrunA17_Chr5g0393511 45.133 113 55 2 11 120 38 146 2.24e-32 112
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGGAGGTGGTGATTCATTC+TGG 0.321260 1_1:-19143512 Msa0012700:CDS
GTTTAAGATGGACAGTAATC+CGG 0.386700 1_1:-19143163 None:intergenic
TACATTTGTTGAAGCGAGTA+AGG 0.420870 1_1:-19143208 Msa0012700:CDS
AAGATGCAGTTGATGCGATT+TGG 0.438269 1_1:-19143293 Msa0012700:CDS
AATGTCAGAGTTTCTTTCCC+AGG 0.450747 1_1:+19143336 None:intergenic
AGACATGGAGGAACCGGTAT+TGG 0.457153 1_1:+19143554 None:intergenic
TGAATGTTCGAACAAATTCA+TGG 0.458298 1_1:-19143532 Msa0012700:CDS
CGAATTCCATTCCACATCAA+TGG 0.458515 1_1:-19143372 Msa0012700:CDS
TGACAGATTGTTGTTTAAGA+TGG 0.463808 1_1:-19143175 Msa0012700:CDS
ACATTCAGACATGGAGGAAC+CGG 0.479236 1_1:+19143548 None:intergenic
GCAGGTTCTACATCAATTTG+AGG 0.490886 1_1:+19143593 None:intergenic
TTTAAGATGGACAGTAATCC+GGG 0.497303 1_1:-19143162 None:intergenic
AAAGATCCATTGATGTGGAA+TGG 0.510519 1_1:+19143366 None:intergenic
TATGTGCAGAAGAAATTCAT+AGG 0.511115 1_1:-19143243 Msa0012700:CDS
GGCTCACGGTAATCATCTGC+AGG 0.521858 1_1:+19143575 None:intergenic
TGCAGAAGAAATTCATAGGA+AGG 0.530136 1_1:-19143239 Msa0012700:CDS
TAGATTACCAGCTTATGAGT+TGG 0.533749 1_1:+19143479 None:intergenic
TTCTTCTGCACATAATCACC+AGG 0.548008 1_1:+19143252 None:intergenic
TTTACGTCCAACTCATAAGC+TGG 0.553121 1_1:-19143486 Msa0012700:intron
CAGAAGAAATTCATAGGAAG+GGG 0.562018 1_1:-19143237 Msa0012700:CDS
TGATTACCGTGAGCCAATAC+CGG 0.564459 1_1:-19143567 Msa0012700:CDS
AGAAACTCTGACATTGACAT+TGG 0.565067 1_1:-19143328 Msa0012700:CDS
AGAAATTCATAGGAAGGGGT+TGG 0.574152 1_1:-19143233 Msa0012700:CDS
TCGAACAAATTCATGGGAGG+TGG 0.578409 1_1:-19143525 Msa0012700:CDS
GAAGCGAGTAAGGTGAAAGT+TGG 0.578483 1_1:-19143198 Msa0012700:CDS
GCAGAAGAAATTCATAGGAA+GGG 0.580030 1_1:-19143238 Msa0012700:CDS
GAATGTTCGAACAAATTCAT+GGG 0.580892 1_1:-19143531 Msa0012700:CDS
CACGAAAAGATCCATTGATG+TGG 0.608405 1_1:+19143361 None:intergenic
TTTGTTCGAACATTCAGACA+TGG 0.613761 1_1:+19143539 None:intergenic
TGTGATATTGATGTTGAACC+TGG 0.629607 1_1:-19143270 Msa0012700:CDS
GAGGAACCGGTATTGGCTCA+CGG 0.632876 1_1:+19143561 None:intergenic
GTTCGAACATTCAGACATGG+AGG 0.688432 1_1:+19143542 None:intergenic
TGTTCGAACAAATTCATGGG+AGG 0.689256 1_1:-19143528 Msa0012700:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTTTATGGTATAATTAAAA+AGG - chr1_1:19143190-19143209 Msa0012700:CDS 15.0%
!! TAGTTAAAATTGTTAAAGAA+AGG - chr1_1:19143284-19143303 Msa0012700:CDS 15.0%
!! AAAATAGTAATATATGAGTT+GGG - chr1_1:19143361-19143380 Msa0012700:CDS 15.0%
!! ACTTTATGGTATAATTAAAA+AGG - chr1_1:19143190-19143209 Msa0012700:CDS 15.0%
!! TAGTTAAAATTGTTAAAGAA+AGG - chr1_1:19143284-19143303 Msa0012700:CDS 15.0%
!! AAAATAGTAATATATGAGTT+GGG - chr1_1:19143361-19143380 Msa0012700:CDS 15.0%
!! TAAAGAGGTAATAAACTTTA+TGG - chr1_1:19143176-19143195 Msa0012700:CDS 20.0%
!! GAAAATAGTAATATATGAGT+TGG - chr1_1:19143360-19143379 Msa0012700:CDS 20.0%
!! TATATAAGTTGCTATGTATT+AGG - chr1_1:19143509-19143528 Msa0012700:CDS 20.0%
!! AATATCAAGTCAACATAAAA+TGG + chr1_1:19143627-19143646 None:intergenic 20.0%
!! TAAAGAGGTAATAAACTTTA+TGG - chr1_1:19143176-19143195 Msa0012700:CDS 20.0%
!! GAAAATAGTAATATATGAGT+TGG - chr1_1:19143360-19143379 Msa0012700:CDS 20.0%
!! TATATAAGTTGCTATGTATT+AGG - chr1_1:19143509-19143528 Msa0012700:CDS 20.0%
!! AATATCAAGTCAACATAAAA+TGG + chr1_1:19143627-19143646 None:intergenic 20.0%
! AAAATTATTGAAAGGGAAAG+AGG - chr1_1:19143254-19143273 Msa0012700:CDS 25.0%
! AAATTATTGAAAGGGAAAGA+GGG - chr1_1:19143255-19143274 Msa0012700:CDS 25.0%
! AAGGTAAAAAGCGAAAAATA+AGG - chr1_1:19143303-19143322 Msa0012700:CDS 25.0%
! GTTGAATAATATAGCAAAAG+TGG - chr1_1:19143334-19143353 Msa0012700:CDS 25.0%
!! TTTGAAACTTAAAACTTGCT+GGG + chr1_1:19143408-19143427 None:intergenic 25.0%
!! ATTTGAAACTTAAAACTTGC+TGG + chr1_1:19143409-19143428 None:intergenic 25.0%
!!! GCAAGTTTTAAGTTTCAAAT+CGG - chr1_1:19143409-19143428 Msa0012700:intron 25.0%
!!! CAAGTTTTAAGTTTCAAATC+GGG - chr1_1:19143410-19143429 Msa0012700:intron 25.0%
! TGAAGAGGTAATAAACTTTA+TGG - chr1_1:19143574-19143593 Msa0012700:CDS 25.0%
! AAAATTATTGAAAGGGAAAG+AGG - chr1_1:19143254-19143273 Msa0012700:CDS 25.0%
! AAATTATTGAAAGGGAAAGA+GGG - chr1_1:19143255-19143274 Msa0012700:CDS 25.0%
! AAGGTAAAAAGCGAAAAATA+AGG - chr1_1:19143303-19143322 Msa0012700:CDS 25.0%
! GTTGAATAATATAGCAAAAG+TGG - chr1_1:19143334-19143353 Msa0012700:CDS 25.0%
!! TTTGAAACTTAAAACTTGCT+GGG + chr1_1:19143408-19143427 None:intergenic 25.0%
!! ATTTGAAACTTAAAACTTGC+TGG + chr1_1:19143409-19143428 None:intergenic 25.0%
!!! GCAAGTTTTAAGTTTCAAAT+CGG - chr1_1:19143409-19143428 Msa0012700:intron 25.0%
!!! CAAGTTTTAAGTTTCAAATC+GGG - chr1_1:19143410-19143429 Msa0012700:intron 25.0%
! TGAAGAGGTAATAAACTTTA+TGG - chr1_1:19143574-19143593 Msa0012700:CDS 25.0%
GTAAAAAGCGAAAAATAAGG+AGG - chr1_1:19143306-19143325 Msa0012700:CDS 30.0%
!! TTGAAACTTAAAACTTGCTG+GGG + chr1_1:19143407-19143426 None:intergenic 30.0%
TTGTAACTCTGTTATGAGAA+TGG - chr1_1:19143545-19143564 Msa0012700:CDS 30.0%
CAATATTCGAATGAAGAATG+AGG - chr1_1:19143696-19143715 Msa0012700:intron 30.0%
TGAATGTTCGAACAAATTCA+TGG - chr1_1:19143793-19143812 Msa0012700:intron 30.0%
GAATGTTCGAACAAATTCAT+GGG - chr1_1:19143794-19143813 Msa0012700:intron 30.0%
TATGTGCAGAAGAAATTCAT+AGG - chr1_1:19144082-19144101 Msa0012700:intron 30.0%
!! TGACAGATTGTTGTTTAAGA+TGG - chr1_1:19144150-19144169 Msa0012700:intron 30.0%
CCTCTTTAATTATTACCCAT+CGG + chr1_1:19143164-19143183 None:intergenic 30.0%
GTAAAAAGCGAAAAATAAGG+AGG - chr1_1:19143306-19143325 Msa0012700:CDS 30.0%
!! TTGAAACTTAAAACTTGCTG+GGG + chr1_1:19143407-19143426 None:intergenic 30.0%
TTGTAACTCTGTTATGAGAA+TGG - chr1_1:19143545-19143564 Msa0012700:CDS 30.0%
CAATATTCGAATGAAGAATG+AGG - chr1_1:19143696-19143715 Msa0012700:intron 30.0%
TGAATGTTCGAACAAATTCA+TGG - chr1_1:19143793-19143812 Msa0012700:intron 30.0%
GAATGTTCGAACAAATTCAT+GGG - chr1_1:19143794-19143813 Msa0012700:intron 30.0%
TATGTGCAGAAGAAATTCAT+AGG - chr1_1:19144082-19144101 Msa0012700:intron 30.0%
!! TGACAGATTGTTGTTTAAGA+TGG - chr1_1:19144150-19144169 Msa0012700:intron 30.0%
AAAAGCGAAAAATAAGGAGG+TGG - chr1_1:19143309-19143328 Msa0012700:CDS 35.0%
AAAGCGAAAAATAAGGAGGT+GGG - chr1_1:19143310-19143329 Msa0012700:CDS 35.0%
TTTGTTCGAACATTCAGACA+TGG + chr1_1:19143789-19143808 None:intergenic 35.0%
TAGATTACCAGCTTATGAGT+TGG + chr1_1:19143849-19143868 None:intergenic 35.0%
AAAGATCCATTGATGTGGAA+TGG + chr1_1:19143962-19143981 None:intergenic 35.0%
AGAAACTCTGACATTGACAT+TGG - chr1_1:19143997-19144016 Msa0012700:intron 35.0%
TGTGATATTGATGTTGAACC+TGG - chr1_1:19144055-19144074 Msa0012700:intron 35.0%
TGCAGAAGAAATTCATAGGA+AGG - chr1_1:19144086-19144105 Msa0012700:intron 35.0%
GCAGAAGAAATTCATAGGAA+GGG - chr1_1:19144087-19144106 Msa0012700:intron 35.0%
CAGAAGAAATTCATAGGAAG+GGG - chr1_1:19144088-19144107 Msa0012700:intron 35.0%
! TACATTTGTTGAAGCGAGTA+AGG - chr1_1:19144117-19144136 Msa0012700:intron 35.0%
AAAAGCGAAAAATAAGGAGG+TGG - chr1_1:19143309-19143328 Msa0012700:CDS 35.0%
AAAGCGAAAAATAAGGAGGT+GGG - chr1_1:19143310-19143329 Msa0012700:CDS 35.0%
TTTGTTCGAACATTCAGACA+TGG + chr1_1:19143789-19143808 None:intergenic 35.0%
TAGATTACCAGCTTATGAGT+TGG + chr1_1:19143849-19143868 None:intergenic 35.0%
AAAGATCCATTGATGTGGAA+TGG + chr1_1:19143962-19143981 None:intergenic 35.0%
AGAAACTCTGACATTGACAT+TGG - chr1_1:19143997-19144016 Msa0012700:intron 35.0%
TGTGATATTGATGTTGAACC+TGG - chr1_1:19144055-19144074 Msa0012700:intron 35.0%
TGCAGAAGAAATTCATAGGA+AGG - chr1_1:19144086-19144105 Msa0012700:intron 35.0%
GCAGAAGAAATTCATAGGAA+GGG - chr1_1:19144087-19144106 Msa0012700:intron 35.0%
CAGAAGAAATTCATAGGAAG+GGG - chr1_1:19144088-19144107 Msa0012700:intron 35.0%
! TACATTTGTTGAAGCGAGTA+AGG - chr1_1:19144117-19144136 Msa0012700:intron 35.0%
AAGCGAAAAATAAGGAGGTG+GGG - chr1_1:19143311-19143330 Msa0012700:CDS 40.0%
CATAACAGAGTTACAAGCTG+TGG + chr1_1:19143541-19143560 None:intergenic 40.0%
TGAGAATGGCAACATTGAAG+AGG - chr1_1:19143559-19143578 Msa0012700:CDS 40.0%
GCAGGTTCTACATCAATTTG+AGG + chr1_1:19143735-19143754 None:intergenic 40.0%
TGTTCGAACAAATTCATGGG+AGG - chr1_1:19143797-19143816 Msa0012700:intron 40.0%
TTTACGTCCAACTCATAAGC+TGG - chr1_1:19143839-19143858 Msa0012700:intron 40.0%
CGAATTCCATTCCACATCAA+TGG - chr1_1:19143953-19143972 Msa0012700:intron 40.0%
CACGAAAAGATCCATTGATG+TGG + chr1_1:19143967-19143986 None:intergenic 40.0%
! AATGGATCTTTTCGTGAACC+TGG - chr1_1:19143971-19143990 Msa0012700:intron 40.0%
! ATGGATCTTTTCGTGAACCT+GGG - chr1_1:19143972-19143991 Msa0012700:intron 40.0%
AATGTCAGAGTTTCTTTCCC+AGG + chr1_1:19143992-19144011 None:intergenic 40.0%
! AAGATGCAGTTGATGCGATT+TGG - chr1_1:19144032-19144051 Msa0012700:intron 40.0%
TTCTTCTGCACATAATCACC+AGG + chr1_1:19144076-19144095 None:intergenic 40.0%
AGAAATTCATAGGAAGGGGT+TGG - chr1_1:19144092-19144111 Msa0012700:intron 40.0%
AAGCGAAAAATAAGGAGGTG+GGG - chr1_1:19143311-19143330 Msa0012700:CDS 40.0%
CATAACAGAGTTACAAGCTG+TGG + chr1_1:19143541-19143560 None:intergenic 40.0%
TGAGAATGGCAACATTGAAG+AGG - chr1_1:19143559-19143578 Msa0012700:CDS 40.0%
GCAGGTTCTACATCAATTTG+AGG + chr1_1:19143735-19143754 None:intergenic 40.0%
TGTTCGAACAAATTCATGGG+AGG - chr1_1:19143797-19143816 Msa0012700:intron 40.0%
TTTACGTCCAACTCATAAGC+TGG - chr1_1:19143839-19143858 Msa0012700:intron 40.0%
CGAATTCCATTCCACATCAA+TGG - chr1_1:19143953-19143972 Msa0012700:intron 40.0%
CACGAAAAGATCCATTGATG+TGG + chr1_1:19143967-19143986 None:intergenic 40.0%
! AATGGATCTTTTCGTGAACC+TGG - chr1_1:19143971-19143990 Msa0012700:intron 40.0%
! ATGGATCTTTTCGTGAACCT+GGG - chr1_1:19143972-19143991 Msa0012700:intron 40.0%
AATGTCAGAGTTTCTTTCCC+AGG + chr1_1:19143992-19144011 None:intergenic 40.0%
! AAGATGCAGTTGATGCGATT+TGG - chr1_1:19144032-19144051 Msa0012700:intron 40.0%
TTCTTCTGCACATAATCACC+AGG + chr1_1:19144076-19144095 None:intergenic 40.0%
AGAAATTCATAGGAAGGGGT+TGG - chr1_1:19144092-19144111 Msa0012700:intron 40.0%
AGCGAAAAATAAGGAGGTGG+GGG - chr1_1:19143312-19143331 Msa0012700:CDS 45.0%
CTTCTCTACATCAACCTTGC+TGG + chr1_1:19143673-19143692 None:intergenic 45.0%
GAAGAATGAGGAAGTTCTGC+AGG - chr1_1:19143708-19143727 Msa0012700:intron 45.0%
TGATTACCGTGAGCCAATAC+CGG - chr1_1:19143758-19143777 Msa0012700:intron 45.0%
ACATTCAGACATGGAGGAAC+CGG + chr1_1:19143780-19143799 None:intergenic 45.0%
GTTCGAACATTCAGACATGG+AGG + chr1_1:19143786-19143805 None:intergenic 45.0%
TCGAACAAATTCATGGGAGG+TGG - chr1_1:19143800-19143819 Msa0012700:intron 45.0%
GAAGCGAGTAAGGTGAAAGT+TGG - chr1_1:19144127-19144146 Msa0012700:intron 45.0%
AGCGAAAAATAAGGAGGTGG+GGG - chr1_1:19143312-19143331 Msa0012700:CDS 45.0%
CTTCTCTACATCAACCTTGC+TGG + chr1_1:19143673-19143692 None:intergenic 45.0%
GAAGAATGAGGAAGTTCTGC+AGG - chr1_1:19143708-19143727 Msa0012700:intron 45.0%
TGATTACCGTGAGCCAATAC+CGG - chr1_1:19143758-19143777 Msa0012700:intron 45.0%
ACATTCAGACATGGAGGAAC+CGG + chr1_1:19143780-19143799 None:intergenic 45.0%
GTTCGAACATTCAGACATGG+AGG + chr1_1:19143786-19143805 None:intergenic 45.0%
TCGAACAAATTCATGGGAGG+TGG - chr1_1:19143800-19143819 Msa0012700:intron 45.0%
GAAGCGAGTAAGGTGAAAGT+TGG - chr1_1:19144127-19144146 Msa0012700:intron 45.0%
!! ATAATAATAAAATTATTGAA+AGG - chr1_1:19143246-19143265 Msa0012700:CDS 5.0%
!! TAATAATAAAATTATTGAAA+GGG - chr1_1:19143247-19143266 Msa0012700:CDS 5.0%
!! ATAAAAATAACATAAAATAA+GGG + chr1_1:19143880-19143899 None:intergenic 5.0%
!! TATAAAAATAACATAAAATA+AGG + chr1_1:19143881-19143900 None:intergenic 5.0%
!! ATAATAATAAAATTATTGAA+AGG - chr1_1:19143246-19143265 Msa0012700:CDS 5.0%
!! TAATAATAAAATTATTGAAA+GGG - chr1_1:19143247-19143266 Msa0012700:CDS 5.0%
!! ATAAAAATAACATAAAATAA+GGG + chr1_1:19143880-19143899 None:intergenic 5.0%
!! TATAAAAATAACATAAAATA+AGG + chr1_1:19143881-19143900 None:intergenic 5.0%
GAGTTGGGCCATAACACAGA+CGG - chr1_1:19143376-19143395 Msa0012700:intron 50.0%
AGTTGGGCCATAACACAGAC+GGG - chr1_1:19143377-19143396 Msa0012700:intron 50.0%
AGACATGGAGGAACCGGTAT+TGG + chr1_1:19143774-19143793 None:intergenic 50.0%
TGGGAGGTGGTGATTCATTC+TGG - chr1_1:19143813-19143832 Msa0012700:intron 50.0%
GAGTTGGGCCATAACACAGA+CGG - chr1_1:19143376-19143395 Msa0012700:intron 50.0%
AGTTGGGCCATAACACAGAC+GGG - chr1_1:19143377-19143396 Msa0012700:intron 50.0%
AGACATGGAGGAACCGGTAT+TGG + chr1_1:19143774-19143793 None:intergenic 50.0%
TGGGAGGTGGTGATTCATTC+TGG - chr1_1:19143813-19143832 Msa0012700:intron 50.0%
TTACTGCAGCGAGTCCAGCA+AGG - chr1_1:19143656-19143675 Msa0012700:intron 55.0%
GGCTCACGGTAATCATCTGC+AGG + chr1_1:19143753-19143772 None:intergenic 55.0%
!! GAGGAACCGGTATTGGCTCA+CGG + chr1_1:19143767-19143786 None:intergenic 55.0%
TTACTGCAGCGAGTCCAGCA+AGG - chr1_1:19143656-19143675 Msa0012700:intron 55.0%
GGCTCACGGTAATCATCTGC+AGG + chr1_1:19143753-19143772 None:intergenic 55.0%
!! GAGGAACCGGTATTGGCTCA+CGG + chr1_1:19143767-19143786 None:intergenic 55.0%
GGGCCATAACACAGACGGGT+CGG - chr1_1:19143381-19143400 Msa0012700:intron 60.0%
GGGCCATAACACAGACGGGT+CGG - chr1_1:19143381-19143400 Msa0012700:intron 60.0%
GGGCCGACCCGTCTGTGTTA+TGG + chr1_1:19143387-19143406 None:intergenic 65.0%
GGGCCGACCCGTCTGTGTTA+TGG + chr1_1:19143387-19143406 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 19143164 19144183 19143164 ID=Msa0012700;Name=Msa0012700
chr1_1 mRNA 19143164 19144183 19143164 ID=Msa0012700-mRNA-1;Parent=Msa0012700;Name=Msa0012700-mRNA-1;_AED=0.12;_eAED=0.12;_QI=0|0|0|0.66|1|1|3|0|122
chr1_1 exon 19144180 19144183 19144180 ID=Msa0012700-mRNA-1:exon:7036;Parent=Msa0012700-mRNA-1
chr1_1 exon 19143487 19143617 19143487 ID=Msa0012700-mRNA-1:exon:7035;Parent=Msa0012700-mRNA-1
chr1_1 exon 19143164 19143394 19143164 ID=Msa0012700-mRNA-1:exon:7034;Parent=Msa0012700-mRNA-1
chr1_1 CDS 19144180 19144183 19144180 ID=Msa0012700-mRNA-1:cds;Parent=Msa0012700-mRNA-1
chr1_1 CDS 19143487 19143617 19143487 ID=Msa0012700-mRNA-1:cds;Parent=Msa0012700-mRNA-1
chr1_1 CDS 19143164 19143394 19143164 ID=Msa0012700-mRNA-1:cds;Parent=Msa0012700-mRNA-1
Gene Sequence

>Msa0012700

ATGGGACCTCAAATTGATGTAGAACCTGCAGATGATTACCGTGAGCCAATACCGGTTCCTCCATGTCTGAATGTTCGAACAAATTCATGGGAGGTGGTGATTCATTCTGGTCATTTACGTCCAACTCATAAGCTGCGAATTCCATTCCACATCAATGGATCTTTTCGTGAACCTGGGAAAGAAACTCTGACATTGACATTGGTTGATTATGAAGAAGATGCAGTTGATGCGATTTGGTGTGATATTGATGTTGAACCTGGTGATTATGTGCAGAAGAAATTCATAGGAAGGGGTTGGCATACATTTGTTGAAGCGAGTAAGGTGAAAGTTGGTGACAGATTGTTGTTTAAGATGGACAGTAATCCG

Protein sequence

>Msa0012700

MGPQIDVEPADDYREPIPVPPCLNVRTNSWEVVIHSGHLRPTHKLRIPFHINGSFREPGKETLTLTLVDYEEDAVDAIWCDIDVEPGDYVQKKFIGRGWHTFVEASKVKVGDRLLFKMDSNP