AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0454370


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0454370 MtrunA17_Chr1g0189271 51.163 129 60 2 1 126 102 230 1.73e-40 137
Msa0454370 MtrunA17_Chr3g0101321 54.264 129 55 2 1 126 258 385 2.51e-39 140
Msa0454370 MtrunA17_Chr1g0189351 51.163 129 60 2 1 126 262 390 3.22e-38 137
Msa0454370 MtrunA17_Chr3g0096971 60.000 95 38 0 1 95 1 95 4.70e-37 124
Msa0454370 MtrunA17_Chr3g0101181 52.308 130 56 3 1 126 274 401 9.85e-37 132
Msa0454370 MtrunA17_Chr3g0100781 57.292 96 41 0 1 96 262 357 8.59e-36 126
Msa0454370 MtrunA17_Chr3g0112511 48.462 130 62 2 1 126 256 384 8.87e-36 130
Msa0454370 MtrunA17_Chr3g0100731 46.897 145 72 2 1 142 20 162 2.13e-35 129
Msa0454370 MtrunA17_Chr3g0100681 48.413 126 62 1 1 123 78 203 8.77e-35 126
Msa0454370 MtrunA17_Chr3g0101211 46.512 129 65 2 1 126 56 183 1.51e-34 124
Msa0454370 MtrunA17_Chr3g0101241 48.462 130 61 3 1 126 243 370 1.91e-34 126
Msa0454370 MtrunA17_Chr3g0101271 48.462 130 61 3 1 126 243 370 1.91e-34 126
Msa0454370 MtrunA17_Chr3g0101331 45.736 129 67 1 1 126 267 395 2.15e-34 126
Msa0454370 MtrunA17_Chr3g0101011 65.591 93 30 2 1 92 276 367 3.18e-34 125
Msa0454370 MtrunA17_Chr3g0101201 49.231 130 60 3 1 126 267 394 4.54e-34 125
Msa0454370 MtrunA17_Chr3g0101231 50.000 130 57 4 1 126 275 400 7.74e-34 124
Msa0454370 MtrunA17_Chr3g0100971 48.413 126 62 1 1 123 262 387 9.40e-34 124
Msa0454370 MtrunA17_Chr3g0100961 45.238 126 66 1 1 123 265 390 7.25e-33 121
Msa0454370 MtrunA17_Chr3g0100801 56.989 93 40 0 1 93 230 322 7.43e-33 121
Msa0454370 MtrunA17_Chr3g0100761 46.825 126 64 1 1 123 222 347 8.46e-33 121
Msa0454370 MtrunA17_Chr1g0151481 45.736 129 67 2 1 126 268 396 4.78e-31 116
Msa0454370 MtrunA17_Chr3g0100851 43.448 145 63 3 1 142 155 283 9.96e-31 115
Msa0454370 MtrunA17_Chr3g0101221 48.462 130 61 3 1 126 269 396 2.01e-28 108
Msa0454370 MtrunA17_Chr3g0100831 42.857 126 64 3 1 123 265 385 3.12e-27 105
Msa0454370 MtrunA17_Chr3g0081391 42.636 129 71 1 1 126 266 394 1.05e-25 101
Msa0454370 MtrunA17_Chr3g0081341 43.077 130 69 2 1 126 330 458 2.60e-25 100
Msa0454370 MtrunA17_Chr3g0080741 41.538 130 70 3 2 127 271 398 1.15e-24 98.6
Msa0454370 MtrunA17_Chr3g0084351 43.750 128 68 3 2 126 194 320 1.01e-23 95.5
Msa0454370 MtrunA17_Chr3g0094301 42.308 130 70 2 1 126 267 395 1.08e-23 95.5
Msa0454370 MtrunA17_Chr7g0229761 42.308 130 70 2 1 126 267 395 1.08e-23 95.5
Msa0454370 MtrunA17_Chr3g0080771 37.241 145 84 3 2 142 268 409 1.48e-23 95.1
Msa0454370 MtrunA17_Chr3g0080791 37.931 145 85 2 1 142 267 409 3.65e-23 94.0
Msa0454370 MtrunA17_Chr5g0444291 42.708 96 35 1 22 97 6 101 3.70e-23 87.0
Msa0454370 MtrunA17_Chr3g0081361 38.889 126 76 1 1 126 275 399 9.75e-23 92.8
Msa0454370 MtrunA17_Chr3g0080631 48.421 95 49 0 1 95 267 361 1.29e-22 92.4
Msa0454370 MtrunA17_Chr3g0080721 38.462 130 75 2 1 126 5 133 2.03e-22 92.0
Msa0454370 MtrunA17_Chr3g0080751 41.085 129 73 1 1 126 267 395 6.86e-22 90.5
Msa0454370 MtrunA17_Chr3g0080601 36.301 146 86 3 1 142 266 408 1.03e-21 90.1
Msa0454370 MtrunA17_Chr3g0081841 48.889 90 46 0 1 90 102 191 1.14e-21 89.7
Msa0454370 MtrunA17_Chr3g0081211 39.231 130 74 2 1 126 265 393 2.17e-21 89.0
Msa0454370 MtrunA17_Chr3g0080731 37.984 129 77 1 1 126 267 395 4.80e-21 88.2
Msa0454370 MtrunA17_Chr3g0081191 37.302 126 78 1 1 126 266 390 9.45e-21 87.0
Msa0454370 MtrunA17_Chr3g0081371 38.889 126 76 1 1 126 275 399 2.05e-20 86.3
Msa0454370 MtrunA17_Chr3g0081181 35.200 125 80 1 6 130 260 383 7.07e-17 76.3
Msa0454370 MtrunA17_Chr5g0410031 33.333 117 78 0 10 126 263 379 1.08e-16 75.5
Msa0454370 MtrunA17_Chr5g0410001 30.769 117 81 0 10 126 262 378 3.17e-16 74.3
Msa0454370 MtrunA17_Chr3g0101391 45.333 75 38 2 55 126 1 75 1.95e-11 60.5
Msa0454370 MtrunA17_Chr5g0404741 28.226 124 86 2 1 123 255 376 2.39e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0454370 AT3G07040.1 32.800 125 78 3 6 126 268 390 5.17e-14 68.2
Msa0454370 AT3G46530.1 34.746 118 64 3 12 123 265 375 9.64e-13 64.7

Find 22 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTCCCTTGTAGTCAAAT+TGG 0.233969 3_3:+45715278 None:intergenic
TTGTCTTCTTTGATGAAGTA+TGG 0.272658 3_3:-45715486 Msa0454370:CDS
TTTATCTCTTGAGGAGTCTA+TGG 0.301690 3_3:-45715323 Msa0454370:CDS
GAACTCTCTTGATTGTGTTC+AGG 0.366315 3_3:+45714913 None:intergenic
ATAGACTCCTCAAGAGATAA+AGG 0.389771 3_3:+45715325 None:intergenic
CTGAGCAACATCCATGTTCC+TGG 0.403982 3_3:+45715390 None:intergenic
TCTTTGATGAAGTATGGAAT+AGG 0.436186 3_3:-45715480 Msa0454370:CDS
TGATGAAGTGAGAAATTACT+TGG 0.438820 3_3:-45715524 Msa0454370:CDS
GATTACAAAGAAAATGTTTG+AGG 0.451115 3_3:-45714849 None:intergenic
TTGACAATAGAAAAGGAAGT+AGG 0.474251 3_3:-45715426 Msa0454370:CDS
CAGGAACATGGATGTTGCTC+AGG 0.479715 3_3:-45715389 Msa0454370:CDS
GGCATTCCAATTTGACTACA+AGG 0.483204 3_3:-45715284 Msa0454370:CDS
TGACAATAGAAAAGGAAGTA+GGG 0.504473 3_3:-45715425 Msa0454370:CDS
GACTACAAGGGAAATTATGA+AGG 0.507454 3_3:-45715271 Msa0454370:intron
AATTCAACCTTTATCTCTTG+AGG 0.511707 3_3:-45715332 Msa0454370:CDS
TATGGAGTTGTTCCGTATGA+AGG 0.511984 3_3:-45715305 Msa0454370:CDS
GAAATTACTTGGAGCAAAAG+AGG 0.524385 3_3:-45715513 Msa0454370:CDS
TTGATTGTGTTCAGGAGCAT+AGG 0.527511 3_3:+45714921 None:intergenic
CAAATTGGAATGCCTTCATA+CGG 0.573239 3_3:+45715293 None:intergenic
ATTTATCACTACCAGGAACA+TGG 0.589487 3_3:-45715401 Msa0454370:CDS
GCATTCCAATTTGACTACAA+GGG 0.629602 3_3:-45715283 Msa0454370:CDS
GTAGGGTATTTATCACTACC+AGG 0.644742 3_3:-45715408 Msa0454370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTATGGAATAGGAATTTT+TGG - chr3_3:45714924-45714943 Msa0454370:CDS 25.0%
!!! GTTTTGATTGACAATAGAAA+AGG - chr3_3:45714962-45714981 Msa0454370:CDS 25.0%
TGATGAAGTGAGAAATTACT+TGG - chr3_3:45714871-45714890 Msa0454370:CDS 30.0%
TTGTCTTCTTTGATGAAGTA+TGG - chr3_3:45714909-45714928 Msa0454370:CDS 30.0%
TCTTTGATGAAGTATGGAAT+AGG - chr3_3:45714915-45714934 Msa0454370:CDS 30.0%
TTGACAATAGAAAAGGAAGT+AGG - chr3_3:45714969-45714988 Msa0454370:intron 30.0%
TGACAATAGAAAAGGAAGTA+GGG - chr3_3:45714970-45714989 Msa0454370:intron 30.0%
AATTCAACCTTTATCTCTTG+AGG - chr3_3:45715063-45715082 Msa0454370:intron 30.0%
AATTTCCCTTGTAGTCAAAT+TGG + chr3_3:45715120-45715139 None:intergenic 30.0%
ATGAAGGTGAGAAAAACATA+AGG - chr3_3:45715140-45715159 Msa0454370:intron 30.0%
TGAAGGTGAGAAAAACATAA+GGG - chr3_3:45715141-45715160 Msa0454370:intron 30.0%
!!! CTCTCTCTATCTGTTTTTAT+CGG + chr3_3:45715278-45715297 None:intergenic 30.0%
AAGACACAAAGCAATTATAC+AGG - chr3_3:45715310-45715329 Msa0454370:CDS 30.0%
GAAATTACTTGGAGCAAAAG+AGG - chr3_3:45714882-45714901 Msa0454370:CDS 35.0%
ATTTATCACTACCAGGAACA+TGG - chr3_3:45714994-45715013 Msa0454370:intron 35.0%
ATAGACTCCTCAAGAGATAA+AGG + chr3_3:45715073-45715092 None:intergenic 35.0%
TTTATCTCTTGAGGAGTCTA+TGG - chr3_3:45715072-45715091 Msa0454370:intron 35.0%
CAAATTGGAATGCCTTCATA+CGG + chr3_3:45715105-45715124 None:intergenic 35.0%
GCATTCCAATTTGACTACAA+GGG - chr3_3:45715112-45715131 Msa0454370:intron 35.0%
GACTACAAGGGAAATTATGA+AGG - chr3_3:45715124-45715143 Msa0454370:intron 35.0%
GAAGGTGAGAAAAACATAAG+GGG - chr3_3:45715142-45715161 Msa0454370:intron 35.0%
!!! TTGCTTTTGAAGTGATGTTC+AGG - chr3_3:45715240-45715259 Msa0454370:intron 35.0%
!! ACATTTTAGTGAACTCTCCT+TGG + chr3_3:45715378-45715397 None:intergenic 35.0%
! GTAGGGTATTTATCACTACC+AGG - chr3_3:45714987-45715006 Msa0454370:intron 40.0%
TATGGAGTTGTTCCGTATGA+AGG - chr3_3:45715090-45715109 Msa0454370:intron 40.0%
GGCATTCCAATTTGACTACA+AGG - chr3_3:45715111-45715130 Msa0454370:intron 40.0%
TTCTCCTAAGCTGAAAACAG+TGG - chr3_3:45715199-45715218 Msa0454370:intron 40.0%
! ACAAGAATACTTCCGGTTTG+TGG + chr3_3:45715342-45715361 None:intergenic 40.0%
! TTGATTGTGTTCAGGAGCAT+AGG + chr3_3:45715477-45715496 None:intergenic 40.0%
GAACTCTCTTGATTGTGTTC+AGG + chr3_3:45715485-45715504 None:intergenic 40.0%
GGTGAGAAAAACATAAGGGG+CGG - chr3_3:45715145-45715164 Msa0454370:intron 45.0%
GTGAGAAAAACATAAGGGGC+GGG - chr3_3:45715146-45715165 Msa0454370:intron 45.0%
TGAGAAAAACATAAGGGGCG+GGG - chr3_3:45715147-45715166 Msa0454370:intron 45.0%
! CTGACCACTGTTTTCAGCTT+AGG + chr3_3:45715206-45715225 None:intergenic 45.0%
TACAGGTTCCTTCCACAAAC+CGG - chr3_3:45715327-45715346 Msa0454370:CDS 45.0%
! GAATACTTCCGGTTTGTGGA+AGG + chr3_3:45715338-45715357 None:intergenic 45.0%
AAGGGGGACAAGAATACTTC+CGG + chr3_3:45715349-45715368 None:intergenic 45.0%
! AACTCTCCTTGGAAGTGCAA+GGG + chr3_3:45715367-45715386 None:intergenic 45.0%
CTGAGCAACATCCATGTTCC+TGG + chr3_3:45715008-45715027 None:intergenic 50.0%
CAGGAACATGGATGTTGCTC+AGG - chr3_3:45715006-45715025 Msa0454370:intron 50.0%
GAGAAAAACATAAGGGGCGG+GGG - chr3_3:45715148-45715167 Msa0454370:intron 50.0%
AGAAAAACATAAGGGGCGGG+GGG - chr3_3:45715149-45715168 Msa0454370:intron 50.0%
! ACTCTCCTTGGAAGTGCAAG+GGG + chr3_3:45715366-45715385 None:intergenic 50.0%
! GAACTCTCCTTGGAAGTGCA+AGG + chr3_3:45715368-45715387 None:intergenic 50.0%
TTGTCCCCCTTGCACTTCCA+AGG - chr3_3:45715358-45715377 Msa0454370:CDS 55.0%
! CTCTCCTTGGAAGTGCAAGG+GGG + chr3_3:45715365-45715384 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 45714851 45715566 45714851 ID=Msa0454370;Name=Msa0454370
chr3_3 mRNA 45714851 45715566 45714851 ID=Msa0454370-mRNA-1;Parent=Msa0454370;Name=Msa0454370-mRNA-1;_AED=0.51;_eAED=0.51;_QI=0|0|0|0.5|1|1|2|0|142
chr3_3 exon 45715272 45715566 45715272 ID=Msa0454370-mRNA-1:exon:8495;Parent=Msa0454370-mRNA-1
chr3_3 exon 45714851 45714984 45714851 ID=Msa0454370-mRNA-1:exon:8494;Parent=Msa0454370-mRNA-1
chr3_3 CDS 45715272 45715566 45715272 ID=Msa0454370-mRNA-1:cds;Parent=Msa0454370-mRNA-1
chr3_3 CDS 45714851 45714984 45714851 ID=Msa0454370-mRNA-1:cds;Parent=Msa0454370-mRNA-1
Gene Sequence

>Msa0454370

ATGGATAGAAGTTTATTAATTGATGAAGTGAGAAATTACTTGGAGCAAAAGAGGTATGTTGTCTTCTTTGATGAAGTATGGAATAGGAATTTTTGGTATGACATGAAATTTGTTTTGATTGACAATAGAAAAGGAAGTAGGGTATTTATCACTACCAGGAACATGGATGTTGCTCAGGTATGTAAAAGATCTTCTTTTGTTCAAGTGCATGAAATTCAACCTTTATCTCTTGAGGAGTCTATGGAGTTGTTCCGTATGAAGGCATTCCAATTTGACTACAAGGGAAATTATGAAGCAAGAGACTTCAAACTGCTCAAGCACAACTGCAAGAGACTTCCTATGCTCCTGAACACAATCAAGAGAGTTCTATTGCTCAAGCACAACTGCAAGAGACTTCTAATCAAACAAGATTACAAAGAAAATGTTTGA

Protein sequence

>Msa0454370

MDRSLLIDEVRNYLEQKRYVVFFDEVWNRNFWYDMKFVLIDNRKGSRVFITTRNMDVAQVCKRSSFVQVHEIQPLSLEESMELFRMKAFQFDYKGNYEARDFKLLKHNCKRLPMLLNTIKRVLLLKHNCKRLLIKQDYKENV*