AlfalfaGEDB | Msa0053010

M. sativa cultivar zhongmu-4 / Msa0053010

KEGG: -
GO: -
PFAM: PF07797.17

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0053010	XP_013465907.1	96.013	301	4	2	1	301	1	293	0.0	581

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0053010	A0A072VEU0	96.013	301	4	2	1	301	1	293	0.0	581

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0007440	Msa0053010	0.837496	5.022830e-57	-8.615850e-47
Msa0042550	Msa0053010	0.802697	4.960143e-49	-8.615850e-47
Msa0053010	Msa0054750	0.808579	2.889830e-50	-8.615850e-47
Msa0053010	Msa0057680	-0.800905	1.157329e-48	-8.615850e-47
Msa0053010	Msa0068740	-0.827551	1.469468e-54	-8.615850e-47
Msa0053010	Msa0096030	0.822459	2.341847e-53	-8.615850e-47
Msa0053010	Msa0098320	0.844552	7.046593e-59	-8.615850e-47
Msa0053010	Msa0098340	0.868407	6.682809e-66	-8.615850e-47
Msa0053010	Msa0105260	0.809178	2.151841e-50	-8.615850e-47
Msa0053010	Msa0187950	0.807767	4.304539e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0053010	MtrunA17_Chr1g0151811	96.013	301	4	2	1	301	1	293	0.0	581
Msa0053010	MtrunA17_Chr7g0253361	41.313	259	96	11	46	301	12	217	1.57e-46	156
Msa0053010	MtrunA17_Chr8g0369051	33.829	269	118	8	51	301	27	253	1.88e-28	110
Msa0053010	MtrunA17_Chr6g0484191	50.847	59	29	0	221	279	169	227	1.34e-14	71.6
Msa0053010	MtrunA17_Chr5g0423331	46.552	58	31	0	220	277	154	211	7.71e-12	63.9
Msa0053010	MtrunA17_Chr5g0423281	51.724	58	27	1	220	277	144	200	9.25e-12	63.5
Msa0053010	MtrunA17_Chr6g0451821	40.789	76	37	1	202	277	168	235	3.02e-11	62.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0053010	AT1G55340.2	80.000	85	16	1	217	301	112	195	3.70e-42	144
Msa0053010	AT1G55340.1	80.000	85	16	1	217	301	122	205	5.62e-42	144
Msa0053010	AT3G03880.1	36.328	256	88	9	51	301	8	193	9.62e-34	122
Msa0053010	AT4G20300.2	42.500	160	71	5	153	301	203	352	1.18e-27	110
Msa0053010	AT4G20300.1	40.141	142	66	4	153	285	203	334	8.54e-22	94.0

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Find 122 sgRNAs with CRISPR-Local

Find 538 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTCTTGCTTTATGCTG+TGG	0.551440	1_2:-7415843	Msa0053010:three_prime_UTR
AAACCCGGTTCAAAGGACAA+CGG	0.698115	1_2:-7422430	Msa0053010:CDS
AAAGATACTTCAAACCGGTT+TGG	0.432467	1_2:-7422375	Msa0053010:CDS
AAAGGTGGAGGGTCATCTTC+TGG	0.286744	1_2:-7418903	Msa0053010:CDS
AAATCGAATATAAATTAACT+TGG	0.462663	1_2:-7415784	Msa0053010:three_prime_UTR
AACCTCCTCCAAGGATTCTC+AGG	0.463865	1_2:-7422248	Msa0053010:intron
AACGCTTTGGATGGGAAGAA+AGG	0.510782	1_2:-7418921	Msa0053010:CDS
AACGGTTCGAGTGGACCGGA+GGG	0.639023	1_2:-7422412	Msa0053010:CDS
AACTTTGGTGGCCACACCGA+TGG	0.505287	1_2:+7418864	None:intergenic
AAGCTTCAAGCTTTAGATGT+CGG	0.528298	1_2:-7415663	Msa0053010:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTATTGAATGGTTAACGCTA+AGG	-	chr1_2:7415683-7415702	Msa0053010:three_prime_UTR	30.0%
TATCTTCATCTTCATCATCT+TGG	-	chr1_2:7416334-7416353	Msa0053010:intron	30.0%
AGTGAATAGTCAAATTCTTC+TGG	+	chr1_2:7416404-7416423	None:intergenic	30.0%
ATTTGCACATAGCATAGAAA+AGG	+	chr1_2:7416766-7416785	None:intergenic	30.0%
GAACTGAATCTCTTCTAATT+TGG	-	chr1_2:7416787-7416806	Msa0053010:intron	30.0%
ATGGATGGTGAAAATTATGA+GGG	+	chr1_2:7416874-7416893	None:intergenic	30.0%
AATGGATGGTGAAAATTATG+AGG	+	chr1_2:7416875-7416894	None:intergenic	30.0%
TTGACACGAAGACAAAAAAA+TGG	+	chr1_2:7416893-7416912	None:intergenic	30.0%
TTATTGTATTGTGCCAATGA+AGG	+	chr1_2:7417236-7417255	None:intergenic	30.0%
AATAGAGACAAAAATGAAGG+AGG	-	chr1_2:7417624-7417643	Msa0053010:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_2	gene	7415553	7424681	7415553	ID=Msa0053010;Name=Msa0053010
chr1_2	mRNA	7415553	7424681	7415553	ID=Msa0053010-mRNA-1;Parent=Msa0053010;Name=Msa0053010-mRNA-1;_AED=0.13;_eAED=0.13;_QI=508\|1\|1\|1\|0.42\|0.37\|8\|362\|301
chr1_2	exon	7415553	7415965	7415553	ID=Msa0053010-mRNA-1:exon:2551;Parent=Msa0053010-mRNA-1
chr1_2	exon	7416245	7416322	7416245	ID=Msa0053010-mRNA-1:exon:2550;Parent=Msa0053010-mRNA-1
chr1_2	exon	7418752	7419103	7418752	ID=Msa0053010-mRNA-1:exon:2549;Parent=Msa0053010-mRNA-1
chr1_2	exon	7422249	7422686	7422249	ID=Msa0053010-mRNA-1:exon:2548;Parent=Msa0053010-mRNA-1
chr1_2	exon	7423807	7423938	7423807	ID=Msa0053010-mRNA-1:exon:2547;Parent=Msa0053010-mRNA-1
chr1_2	exon	7424106	7424200	7424106	ID=Msa0053010-mRNA-1:exon:2546;Parent=Msa0053010-mRNA-1
chr1_2	exon	7424303	7424389	7424303	ID=Msa0053010-mRNA-1:exon:2545;Parent=Msa0053010-mRNA-1
chr1_2	exon	7424501	7424681	7424501	ID=Msa0053010-mRNA-1:exon:2544;Parent=Msa0053010-mRNA-1
chr1_2	five_prime_UTR	7424501	7424681	7424501	ID=Msa0053010-mRNA-1:five_prime_utr;Parent=Msa0053010-mRNA-1
chr1_2	five_prime_UTR	7424303	7424389	7424303	ID=Msa0053010-mRNA-1:five_prime_utr;Parent=Msa0053010-mRNA-1
chr1_2	five_prime_UTR	7424106	7424200	7424106	ID=Msa0053010-mRNA-1:five_prime_utr;Parent=Msa0053010-mRNA-1
chr1_2	five_prime_UTR	7423807	7423938	7423807	ID=Msa0053010-mRNA-1:five_prime_utr;Parent=Msa0053010-mRNA-1
chr1_2	five_prime_UTR	7422674	7422686	7422674	ID=Msa0053010-mRNA-1:five_prime_utr;Parent=Msa0053010-mRNA-1
chr1_2	CDS	7422249	7422673	7422249	ID=Msa0053010-mRNA-1:cds;Parent=Msa0053010-mRNA-1
chr1_2	CDS	7418752	7419103	7418752	ID=Msa0053010-mRNA-1:cds;Parent=Msa0053010-mRNA-1
chr1_2	CDS	7416245	7416322	7416245	ID=Msa0053010-mRNA-1:cds;Parent=Msa0053010-mRNA-1
chr1_2	CDS	7415915	7415965	7415915	ID=Msa0053010-mRNA-1:cds;Parent=Msa0053010-mRNA-1
chr1_2	three_prime_UTR	7415553	7415914	7415553	ID=Msa0053010-mRNA-1:three_prime_utr;Parent=Msa0053010-mRNA-1

Gene Sequence

>Msa0053010

ATGGATAAGGAATCACATAACAACCATGACAACAGCAACAACATTGCTAGTTTAAGAGATGATAATAGAGACAAAAATGAAGGAGGGGATGGATCTAGTTCCTTGTTATTGAGGGCTAGCTCAGATGGATTCAGACAATCATCCACGTCAGATTTGGTGTTACAATGGGGGAACAGGAAGCGATTAAGGTGCATGAAGGTACAAGTTAAACAAGATTCTTTAAACCCGGTTCAAAGGACAACGGTTCGAGTGGACCGGAGGGTAATTAGGACCGAGAAAGATACTTCAAACCGGTTTGGAACATCCCACACTGATAATAACATTGTGAATGGTCATAATCATAATAATCAAAATCAGAGTAACGGGTATCCTAGTCTTCGTCAACGTGCCCCTTCTCCACAGCAACCTCCTCCAAGGATTCTCAGGAACACAGAAGGTTCAGGTGCTATGAGAGGAACACAAACCAACGGAAGTGTTAGGGGAATTGCCTCACCAGAGAGGGTTGGACATGAGAAAAGAGGGACCCACACCCATCACAATGAACACAATAACAACAACAACAACAAATCTGCAGCTTCATCTGATAACGCTTTGGATGGGAAGAAAGGTGGAGGGTCATCTTCTGGGAGTGGTGATGCTACTCCATCGGTGTGGCCACCAAAGTTTGTGATTGCCTTGACTAACAAGGAGAAAGAAGAAGATTTTATGGCTATTAAGGGTTCTAAGCTGCCTCAAAGACCTAAGAAAAGAGCCAAATTGATACAACGTACCCTCAATCTTGTAAGTCCAGGTACATGGCTATCCGATCTTACCTTGGAACGATATGAAGTTAGGGAGAAGAAAATAACCAAAAAGAGACCAAGAGGCTTAAAAGCAATGGGTAACATGGACTCTGACTCTGAATAG

Protein sequence

>Msa0053010

MDKESHNNHDNSNNIASLRDDNRDKNEGGDGSSSLLLRASSDGFRQSSTSDLVLQWGNRKRLRCMKVQVKQDSLNPVQRTTVRVDRRVIRTEKDTSNRFGTSHTDNNIVNGHNHNNQNQSNGYPSLRQRAPSPQQPPPRILRNTEGSGAMRGTQTNGSVRGIASPERVGHEKRGTHTHHNEHNNNNNNKSAASSDNALDGKKGGGSSSGSGDATPSVWPPKFVIALTNKEKEEDFMAIKGSKLPQRPKKRAKLIQRTLNLVSPGTWLSDLTLERYEVREKKITKKRPRGLKAMGNMDSDSE*