Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0925810 | KEH27251.1 | 69.811 | 159 | 32 | 2 | 43 | 185 | 643 | 801 | 1.42e-60 | 213 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0045790 | Msa0925810 | 0.804152 | 2.477572e-49 | -8.615850e-47 |
| Msa0053010 | Msa0925810 | 0.820746 | 5.829110e-53 | -8.615850e-47 |
| Msa0055740 | Msa0925810 | 0.812146 | 4.909430e-51 | -8.615850e-47 |
| Msa0607540 | Msa0925810 | 0.815008 | 1.152214e-51 | -8.615850e-47 |
| Msa0649110 | Msa0925810 | 0.825535 | 4.445745e-54 | -8.615850e-47 |
| Msa0652940 | Msa0925810 | 0.823186 | 1.586240e-53 | -8.615850e-47 |
| Msa0657970 | Msa0925810 | 0.801343 | 9.417978e-49 | -8.615850e-47 |
| Msa0675690 | Msa0925810 | 0.810488 | 1.124163e-50 | -8.615850e-47 |
| Msa0688270 | Msa0925810 | 0.804008 | 2.654430e-49 | -8.615850e-47 |
| Msa0706020 | Msa0925810 | 0.800117 | 1.674924e-48 | -8.615850e-47 |
| Msa0731940 | Msa0925810 | 0.802762 | 4.810466e-49 | -8.615850e-47 |
| Msa0766630 | Msa0925810 | 0.826921 | 2.080254e-54 | -8.615850e-47 |
| Msa0790600 | Msa0925810 | 0.802817 | 4.686197e-49 | -8.615850e-47 |
| Msa0802010 | Msa0925810 | 0.810577 | 1.075519e-50 | -8.615850e-47 |
| Msa0807030 | Msa0925810 | 0.807401 | 5.145699e-50 | -8.615850e-47 |
| Msa0128020 | Msa0925810 | 0.810528 | 1.102224e-50 | -8.615850e-47 |
| Msa0137640 | Msa0925810 | 0.800170 | 1.633993e-48 | -8.615850e-47 |
| Msa0153910 | Msa0925810 | 0.803431 | 3.497113e-49 | -8.615850e-47 |
| Msa0195820 | Msa0925810 | 0.812112 | 4.994385e-51 | -8.615850e-47 |
| Msa0202720 | Msa0925810 | 0.822154 | 2.756542e-53 | -8.615850e-47 |
| Msa0214300 | Msa0925810 | 0.807754 | 4.330882e-50 | -8.615850e-47 |
| Msa0379640 | Msa0925810 | 0.801064 | 1.074001e-48 | -8.615850e-47 |
| Msa0390400 | Msa0925810 | -0.804473 | 2.123856e-49 | -8.615850e-47 |
| Msa0411390 | Msa0925810 | 0.827409 | 1.589976e-54 | -8.615850e-47 |
| Msa0434000 | Msa0925810 | 0.801731 | 7.841649e-49 | -8.615850e-47 |
| Msa0434180 | Msa0925810 | 0.802006 | 6.884787e-49 | -8.615850e-47 |
| Msa0440400 | Msa0925810 | 0.801083 | 1.064472e-48 | -8.615850e-47 |
| Msa0453640 | Msa0925810 | 0.806204 | 9.214236e-50 | -8.615850e-47 |
| Msa0469940 | Msa0925810 | -0.800081 | 1.703367e-48 | -8.615850e-47 |
| Msa0265670 | Msa0925810 | 0.804108 | 2.530504e-49 | -8.615850e-47 |
| Msa0285450 | Msa0925810 | 0.805763 | 1.141155e-49 | -8.615850e-47 |
| Msa0305190 | Msa0925810 | 0.813759 | 2.175553e-51 | -8.615850e-47 |
| Msa0315730 | Msa0925810 | 0.806085 | 9.760686e-50 | -8.615850e-47 |
| Msa0363790 | Msa0925810 | 0.801364 | 9.325531e-49 | -8.615850e-47 |
| Msa0493250 | Msa0925810 | 0.825967 | 3.511697e-54 | -8.615850e-47 |
| Msa0880950 | Msa0925810 | 0.814323 | 1.633441e-51 | -8.615850e-47 |
| Msa0885280 | Msa0925810 | 0.820128 | 8.080165e-53 | -8.615850e-47 |
| Msa0886330 | Msa0925810 | -0.835896 | 1.284751e-56 | -8.615850e-47 |
| Msa0900980 | Msa0925810 | 0.811949 | 5.420543e-51 | -8.615850e-47 |
| Msa0901400 | Msa0925810 | 0.848802 | 4.857983e-60 | -8.615850e-47 |
| Msa0916690 | Msa0925810 | 0.812593 | 3.920584e-51 | -8.615850e-47 |
| Msa0923010 | Msa0925810 | 0.801456 | 8.927774e-49 | -8.615850e-47 |
| Msa0925810 | Msa0925870 | 0.842281 | 2.846081e-58 | -8.615850e-47 |
| Msa0925810 | Msa0927290 | 0.803141 | 4.017139e-49 | -8.615850e-47 |
| Msa0925810 | Msa0935670 | 0.819262 | 1.273480e-52 | -8.615850e-47 |
| Msa0925810 | Msa0935910 | 0.834710 | 2.561114e-56 | -8.615850e-47 |
| Msa0925810 | Msa0967280 | 0.802344 | 5.864677e-49 | -8.615850e-47 |
| Msa0925810 | Msa0976670 | 0.819582 | 1.076949e-52 | -8.615850e-47 |
| Msa0925810 | Msa0989360 | 0.806403 | 8.366794e-50 | -8.615850e-47 |
| Msa0925810 | Msa1001180 | 0.825508 | 4.511106e-54 | -8.615850e-47 |
| Msa0925810 | Msa1014540 | 0.814064 | 1.863733e-51 | -8.615850e-47 |
| Msa0925810 | Msa1023950 | 0.804374 | 2.227231e-49 | -8.615850e-47 |
| Msa0925810 | Msa1058710 | 0.818788 | 1.632639e-52 | -8.615850e-47 |
| Msa0925810 | Msa1061680 | 0.802563 | 5.287355e-49 | -8.615850e-47 |
| Msa0925810 | Msa1075650 | 0.831526 | 1.588398e-55 | -8.615850e-47 |
| Msa0925810 | Msa1076700 | 0.821727 | 3.462189e-53 | -8.615850e-47 |
| Msa0925810 | Msa1082500 | 0.804080 | 2.564082e-49 | -8.615850e-47 |
| Msa0925810 | Msa1089040 | 0.805160 | 1.526528e-49 | -8.615850e-47 |
| Msa0925810 | Msa1108760 | 0.817751 | 2.802969e-52 | -8.615850e-47 |
| Msa0925810 | Msa1249490 | 0.811653 | 6.287543e-51 | -8.615850e-47 |
| Msa0925810 | Msa1282580 | -0.821438 | 4.037697e-53 | -8.615850e-47 |
| Msa0925810 | Msa1287460 | 0.807921 | 3.991932e-50 | -8.615850e-47 |
| Msa0925810 | Msa1390050 | 0.803611 | 3.208942e-49 | -8.615850e-47 |
| Msa0925810 | Msa1409700 | 0.809180 | 2.149758e-50 | -8.615850e-47 |
| Msa0925810 | Msa1420820 | 0.803445 | 3.473838e-49 | -8.615850e-47 |
| Msa0925810 | Msa1436480 | 0.802948 | 4.402569e-49 | -8.615850e-47 |
| Msa0925810 | Msa1466540 | 0.813651 | 2.297365e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0925810 | MtrunA17_Chr6g0487991 | 69.811 | 159 | 32 | 2 | 43 | 185 | 708 | 866 | 1.95e-64 | 213 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 29 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGATCTGGTTTCAGTATT+AGG | 0.243148 | 6_2:-69121496 | None:intergenic |
| GCCTGATGTTGTAGAGTTAT+TGG | 0.246788 | 6_2:-69121470 | None:intergenic |
| TCAGTATCCTCAAAATTATT+AGG | 0.251875 | 6_2:-69121694 | None:intergenic |
| ATTTCTATTTCCTCGGCTTT+CGG | 0.284663 | 6_2:-69121935 | None:intergenic |
| TCTGATGTATTCTTAATATA+AGG | 0.320184 | 6_2:-69121808 | None:intergenic |
| ATTGAAGATGGTTCATTAGT+TGG | 0.352517 | 6_2:-69121646 | None:intergenic |
| CTTGAGAATGCCTGCTTATG+AGG | 0.372046 | 6_2:+69121301 | None:intergenic |
| GATGTATATTTGTCCTGAAC+AGG | 0.381263 | 6_2:-69121913 | None:intergenic |
| AATACTGAAACCAGATCTTC+AGG | 0.417052 | 6_2:+69121499 | Msa0925810:CDS |
| GCCAATAACTCTACAACATC+AGG | 0.419000 | 6_2:+69121469 | Msa0925810:CDS |
| ACTAATGAACCATCTTCAAT+AGG | 0.420020 | 6_2:+69121649 | Msa0925810:CDS |
| CTGAACAGGAGCATCAAAAC+TGG | 0.438709 | 6_2:-69121899 | None:intergenic |
| AGCCGACATTTCTATTTCCT+CGG | 0.444211 | 6_2:-69121942 | None:intergenic |
| GGTAGAACATGAACTTGAAA+AGG | 0.477418 | 6_2:-69121326 | None:intergenic |
| GAGTTTCTTCCTATTGAAGA+TGG | 0.481300 | 6_2:-69121658 | None:intergenic |
| TGATTCTGATAGCTACGATC+TGG | 0.490408 | 6_2:+69121987 | Msa0925810:CDS |
| ATTAAGCTACAGAACGTTGA+AGG | 0.508639 | 6_2:+69121753 | Msa0925810:CDS |
| GAGAAATCTTGCGTAGAAAG+TGG | 0.543605 | 6_2:+69121724 | Msa0925810:CDS |
| AGATGATGTTTCAACTTCAG+TGG | 0.545104 | 6_2:+69121828 | Msa0925810:CDS |
| GATGATGTTTCAACTTCAGT+GGG | 0.546176 | 6_2:+69121829 | Msa0925810:CDS |
| TGAAAAGGTTCCTCATAAGC+AGG | 0.558111 | 6_2:-69121311 | None:intergenic |
| CTGCACACAATCTTTCACTC+TGG | 0.560209 | 6_2:-69121864 | None:intergenic |
| ATCTTGCGTAGAAAGTGGTA+TGG | 0.560415 | 6_2:+69121729 | Msa0925810:CDS |
| GATGTATTCTTAATATAAGG+TGG | 0.589065 | 6_2:-69121805 | None:intergenic |
| AGCCGAGGAAATAGAAATGT+CGG | 0.590279 | 6_2:+69121940 | Msa0925810:CDS |
| AGCTACAGAACGTTGAAGGG+TGG | 0.614968 | 6_2:+69121757 | Msa0925810:CDS |
| TTAAGCTACAGAACGTTGAA+GGG | 0.632705 | 6_2:+69121754 | Msa0925810:CDS |
| AAATATACATCCGAAAGCCG+AGG | 0.671474 | 6_2:+69121925 | Msa0925810:CDS |
| GTGAAAGATTGTGTGCAGTG+AGG | 0.767939 | 6_2:+69121869 | Msa0925810:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGAATCTCAAATAATTC+AGG | - | chr6_2:69121582-69121601 | None:intergenic | 20.0% |
| !! | TCTGATGTATTCTTAATATA+AGG | - | chr6_2:69121811-69121830 | None:intergenic | 20.0% |
| ! | GTGGAAGTCAAAAAAAATTA+TGG | + | chr6_2:69121610-69121629 | Msa0925810:CDS | 25.0% |
| ! | TCAGTATCCTCAAAATTATT+AGG | - | chr6_2:69121697-69121716 | None:intergenic | 25.0% |
| ! | GATGTATTCTTAATATAAGG+TGG | - | chr6_2:69121808-69121827 | None:intergenic | 25.0% |
| TTGTGTTGTAGATGTGAAAT+AGG | - | chr6_2:69121377-69121396 | None:intergenic | 30.0% | |
| TTTGTATATGCATCATGCAT+TGG | - | chr6_2:69121406-69121425 | None:intergenic | 30.0% | |
| !! | TAAAAAAGTACCTGAAGATC+TGG | - | chr6_2:69121512-69121531 | None:intergenic | 30.0% |
| !! | TTATTTGAGATTCTTTTCGC+AGG | + | chr6_2:69121585-69121604 | Msa0925810:intron | 30.0% |
| ATTGAAGATGGTTCATTAGT+TGG | - | chr6_2:69121649-69121668 | None:intergenic | 30.0% | |
| ACTAATGAACCATCTTCAAT+AGG | + | chr6_2:69121649-69121668 | Msa0925810:CDS | 30.0% | |
| !! | TTTTGAGGATACTGAAACTT+TGG | + | chr6_2:69121702-69121721 | Msa0925810:CDS | 30.0% |
| GGTAGAACATGAACTTGAAA+AGG | - | chr6_2:69121329-69121348 | None:intergenic | 35.0% | |
| TGTTGTAGATGTGAAATAGG+AGG | - | chr6_2:69121374-69121393 | None:intergenic | 35.0% | |
| !! | TATGCATCATGCATTGGTTT+AGG | - | chr6_2:69121400-69121419 | None:intergenic | 35.0% |
| ! | TCTAAATACTGCTGCTTGAA+AGG | + | chr6_2:69121441-69121460 | Msa0925810:intron | 35.0% |
| ! | GAAGATCTGGTTTCAGTATT+AGG | - | chr6_2:69121499-69121518 | None:intergenic | 35.0% |
| AATACTGAAACCAGATCTTC+AGG | + | chr6_2:69121499-69121518 | Msa0925810:CDS | 35.0% | |
| GAGTTTCTTCCTATTGAAGA+TGG | - | chr6_2:69121661-69121680 | None:intergenic | 35.0% | |
| ! | GCAGTCACCTAATAATTTTG+AGG | + | chr6_2:69121687-69121706 | Msa0925810:CDS | 35.0% |
| ATTAAGCTACAGAACGTTGA+AGG | + | chr6_2:69121753-69121772 | Msa0925810:CDS | 35.0% | |
| TTAAGCTACAGAACGTTGAA+GGG | + | chr6_2:69121754-69121773 | Msa0925810:CDS | 35.0% | |
| AGATGATGTTTCAACTTCAG+TGG | + | chr6_2:69121828-69121847 | Msa0925810:CDS | 35.0% | |
| GATGATGTTTCAACTTCAGT+GGG | + | chr6_2:69121829-69121848 | Msa0925810:CDS | 35.0% | |
| GATGTATATTTGTCCTGAAC+AGG | - | chr6_2:69121916-69121935 | None:intergenic | 35.0% | |
| ATTTCTATTTCCTCGGCTTT+CGG | - | chr6_2:69121938-69121957 | None:intergenic | 35.0% | |
| TGAAAAGGTTCCTCATAAGC+AGG | - | chr6_2:69121314-69121333 | None:intergenic | 40.0% | |
| GCCAATAACTCTACAACATC+AGG | + | chr6_2:69121469-69121488 | Msa0925810:CDS | 40.0% | |
| GCCTGATGTTGTAGAGTTAT+TGG | - | chr6_2:69121473-69121492 | None:intergenic | 40.0% | |
| !! | TTTGAGATTCTTTTCGCAGG+AGG | + | chr6_2:69121588-69121607 | Msa0925810:intron | 40.0% |
| GAGAAATCTTGCGTAGAAAG+TGG | + | chr6_2:69121724-69121743 | Msa0925810:CDS | 40.0% | |
| ATCTTGCGTAGAAAGTGGTA+TGG | + | chr6_2:69121729-69121748 | Msa0925810:CDS | 40.0% | |
| ! | AAGGGTGGTTTTCGCAATAA+AGG | + | chr6_2:69121772-69121791 | Msa0925810:CDS | 40.0% |
| AAATATACATCCGAAAGCCG+AGG | + | chr6_2:69121925-69121944 | Msa0925810:CDS | 40.0% | |
| AGCCGAGGAAATAGAAATGT+CGG | + | chr6_2:69121940-69121959 | Msa0925810:CDS | 40.0% | |
| AGCCGACATTTCTATTTCCT+CGG | - | chr6_2:69121945-69121964 | None:intergenic | 40.0% | |
| TGATTCTGATAGCTACGATC+TGG | + | chr6_2:69121987-69122006 | Msa0925810:CDS | 40.0% | |
| ! | CGCTTTTCATCATGAACAGG+TGG | - | chr6_2:69121350-69121369 | None:intergenic | 45.0% |
| ! | GGACGCTTTTCATCATGAAC+AGG | - | chr6_2:69121353-69121372 | None:intergenic | 45.0% |
| CTGCACACAATCTTTCACTC+TGG | - | chr6_2:69121867-69121886 | None:intergenic | 45.0% | |
| GTGAAAGATTGTGTGCAGTG+AGG | + | chr6_2:69121869-69121888 | Msa0925810:CDS | 45.0% | |
| !! | CTGAACAGGAGCATCAAAAC+TGG | - | chr6_2:69121902-69121921 | None:intergenic | 45.0% |
| !! | CAGTTTTGATGCTCCTGTTC+AGG | + | chr6_2:69121900-69121919 | Msa0925810:CDS | 45.0% |
| ! | GAGATTCTTTTCGCAGGAGG+TGG | + | chr6_2:69121591-69121610 | Msa0925810:intron | 50.0% |
| AGCTACAGAACGTTGAAGGG+TGG | + | chr6_2:69121757-69121776 | Msa0925810:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_2 | gene | 69121308 | 69122050 | 69121308 | ID=Msa0925810;Name=Msa0925810 |
| chr6_2 | mRNA | 69121308 | 69122050 | 69121308 | ID=Msa0925810-mRNA-1;Parent=Msa0925810;Name=Msa0925810-mRNA-1;_AED=0.47;_eAED=0.52;_QI=0|0|0|1|1|1|3|0|185 |
| chr6_2 | exon | 69121308 | 69121368 | 69121308 | ID=Msa0925810-mRNA-1:exon:11161;Parent=Msa0925810-mRNA-1 |
| chr6_2 | exon | 69121468 | 69121520 | 69121468 | ID=Msa0925810-mRNA-1:exon:11162;Parent=Msa0925810-mRNA-1 |
| chr6_2 | exon | 69121607 | 69122050 | 69121607 | ID=Msa0925810-mRNA-1:exon:11163;Parent=Msa0925810-mRNA-1 |
| chr6_2 | CDS | 69121308 | 69121368 | 69121308 | ID=Msa0925810-mRNA-1:cds;Parent=Msa0925810-mRNA-1 |
| chr6_2 | CDS | 69121468 | 69121520 | 69121468 | ID=Msa0925810-mRNA-1:cds;Parent=Msa0925810-mRNA-1 |
| chr6_2 | CDS | 69121607 | 69122050 | 69121607 | ID=Msa0925810-mRNA-1:cds;Parent=Msa0925810-mRNA-1 |
| Gene Sequence |
| Protein sequence |
