AlfalfaGEDB | Msa1396400

M. sativa cultivar zhongmu-4 / Msa1396400

KEGG: K06632
GO: GO:0000075 GO:0000076 GO:0003674 GO:0003824 GO:0004672 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007049 GO:0007050 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0031570 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0045786 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051726 GO:0065007 GO:0071704 GO:0140096 GO:1901564
PFAM: PF00069.28

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1396400	XP_003625897.1	97.959	490	7	1	1	487	1	490	0.0	999

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1396400	sp\|Q8L4H0\|WEE1_ARATH	59.138	487	187	6	2	480	15	497	0.0	589

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1396400	G7KRU4	97.959	490	7	1	1	487	1	490	0.0	999

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0001830	Msa1396400	-0.826459	2.681064e-54	-8.615850e-47
Msa0004840	Msa1396400	-0.807754	4.330468e-50	-8.615850e-47
Msa0007440	Msa1396400	-0.858681	6.969662e-63	-8.615850e-47
Msa0007470	Msa1396400	-0.807900	4.032954e-50	-8.615850e-47
Msa0010490	Msa1396400	-0.864343	1.301994e-64	-8.615850e-47
Msa0010500	Msa1396400	-0.855650	5.471200e-62	-8.615850e-47
Msa0015090	Msa1396400	-0.823579	1.283780e-53	-8.615850e-47
Msa0016510	Msa1396400	-0.810964	8.869233e-51	-8.615850e-47
Msa0017320	Msa1396400	-0.809097	2.238610e-50	-8.615850e-47
Msa0018070	Msa1396400	-0.809894	1.510093e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1396400	MtrunA17_Chr7g0269001	97.959	490	7	1	1	487	1	490	0.0	999
Msa1396400	MtrunA17_Chr8g0369451	26.690	281	168	6	235	478	4	283	1.69e-21	97.1
Msa1396400	MtrunA17_Chr5g0409421	26.042	288	171	7	228	478	1	283	2.36e-21	96.7
Msa1396400	MtrunA17_Chr1g0173921	29.730	259	161	12	234	476	3	256	3.85e-20	94.4
Msa1396400	MtrunA17_Chr2g0286501	31.373	255	151	10	235	472	49	296	3.86e-20	94.4
Msa1396400	MtrunA17_Chr8g0386981	32.950	261	148	14	241	481	414	667	1.34e-19	92.4
Msa1396400	MtrunA17_Chr4g0051191	28.682	258	169	10	241	485	169	424	1.39e-19	90.9
Msa1396400	MtrunA17_Chr8g0374701	29.670	273	144	11	239	476	129	388	2.22e-19	91.3
Msa1396400	MtrunA17_Chr1g0157131	27.757	263	155	9	239	476	78	330	2.31e-19	90.9
Msa1396400	MtrunA17_Chr4g0059951	26.648	349	211	12	161	485	11	338	4.00e-19	90.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1396400	AT1G02970.1	59.138	487	187	6	2	480	15	497	0.0	589
Msa1396400	AT4G19110.1	26.978	278	165	6	238	478	7	283	5.44e-23	102
Msa1396400	AT4G19110.2	26.978	278	165	6	238	478	7	283	5.61e-23	102
Msa1396400	AT5G45430.2	26.619	278	166	6	238	478	7	283	1.15e-20	95.1
Msa1396400	AT5G45430.1	26.619	278	166	6	238	478	7	283	1.23e-20	95.1
Msa1396400	AT3G20860.1	29.851	268	170	11	234	487	3	266	3.48e-20	93.2
Msa1396400	AT1G49580.1	30.961	281	148	12	239	487	154	420	8.58e-20	92.8
Msa1396400	AT3G19100.1	30.000	270	144	11	239	476	148	404	1.47e-19	92.4
Msa1396400	AT1G76040.2	29.434	265	156	11	234	476	113	368	1.93e-19	91.7
Msa1396400	AT1G76040.3	29.434	265	156	11	234	476	113	368	2.04e-19	91.3

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Find 139 sgRNAs with CRISPR-Local

Find 339 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCAAAAGATGTCGACT+GGG	0.647827	tig0022022:+71257	Msa1396400:CDS
AAAATCAAGGGGAAGGAAGA+AGG	0.477940	tig0022022:+70709	Msa1396400:CDS
AAACAACAGGCAAACATTTC+TGG	0.179440	tig0022022:-75431	None:intergenic
AAACCCCAAAATCAAGGGGA+AGG	0.559167	tig0022022:+70702	Msa1396400:CDS
AAAGTATTAAAGCGAATAGA+TGG	0.379092	tig0022022:+73414	Msa1396400:CDS
AAATAGGGGTTACTAAAGCA+AGG	0.563788	tig0022022:-72985	None:intergenic
AAATATCAACCTTGTCAAGG+TGG	0.623552	tig0022022:-74245	None:intergenic
AAATCAAAATCCTTCCATTA+GGG	0.376468	tig0022022:+70862	Msa1396400:CDS
AAATCACCTATGCCAGAATC+AGG	0.426205	tig0022022:+74303	Msa1396400:CDS
AACCAAAGATCCAAATGTGC+AGG	0.530641	tig0022022:+73045	Msa1396400:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTATTCTTCATCTTCTTCAC+TGG	+	tig0022022:70640-70659	Msa1396400:five_prime_UTR	30.0%
CAAATCAAAATCCTTCCATT+AGG	+	tig0022022:70861-70880	Msa1396400:CDS	30.0%
TTCTTAGTCAAGATTTCTTC+TGG	+	tig0022022:70924-70943	Msa1396400:CDS	30.0%
CTCAATTTCGGAAAATGAAA+AGG	-	tig0022022:70967-70986	None:intergenic	30.0%
CAAACTTCGAAACCAAAAAA+TGG	-	tig0022022:70996-71015	None:intergenic	30.0%
TAGTAGTATTCAATTTCTCC+GGG	-	tig0022022:71238-71257	None:intergenic	30.0%
GATGACATACTTCTTGAATA+TGG	-	tig0022022:71729-71748	None:intergenic	30.0%
AGAATAATCCGATGATTTCA+GGG	-	tig0022022:71861-71880	None:intergenic	30.0%
TAGAATAATCCGATGATTTC+AGG	-	tig0022022:71862-71881	None:intergenic	30.0%
GGACTAAACATATGTTCTAT+CGG	+	tig0022022:72022-72041	Msa1396400:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0022022	gene	70620	75481	70620	ID=Msa1396400;Name=Msa1396400
tig0022022	mRNA	70620	75481	70620	ID=Msa1396400-mRNA-1;Parent=Msa1396400;Name=Msa1396400-mRNA-1;_AED=0.12;_eAED=0.12;_QI=72\|1\|1\|1\|1\|1\|10\|288\|487
tig0022022	exon	70620	70945	70620	ID=Msa1396400-mRNA-1:exon:15450;Parent=Msa1396400-mRNA-1
tig0022022	exon	71047	71107	71047	ID=Msa1396400-mRNA-1:exon:15451;Parent=Msa1396400-mRNA-1
tig0022022	exon	71212	71278	71212	ID=Msa1396400-mRNA-1:exon:15452;Parent=Msa1396400-mRNA-1
tig0022022	exon	72800	73066	72800	ID=Msa1396400-mRNA-1:exon:15453;Parent=Msa1396400-mRNA-1
tig0022022	exon	73205	73272	73205	ID=Msa1396400-mRNA-1:exon:15454;Parent=Msa1396400-mRNA-1
tig0022022	exon	73381	73487	73381	ID=Msa1396400-mRNA-1:exon:15455;Parent=Msa1396400-mRNA-1
tig0022022	exon	73562	73602	73562	ID=Msa1396400-mRNA-1:exon:15456;Parent=Msa1396400-mRNA-1
tig0022022	exon	73708	73856	73708	ID=Msa1396400-mRNA-1:exon:15457;Parent=Msa1396400-mRNA-1
tig0022022	exon	74051	74404	74051	ID=Msa1396400-mRNA-1:exon:15458;Parent=Msa1396400-mRNA-1
tig0022022	exon	75098	75481	75098	ID=Msa1396400-mRNA-1:exon:15459;Parent=Msa1396400-mRNA-1
tig0022022	five_prime_UTR	70620	70691	70620	ID=Msa1396400-mRNA-1:five_prime_utr;Parent=Msa1396400-mRNA-1
tig0022022	CDS	70692	70945	70692	ID=Msa1396400-mRNA-1:cds;Parent=Msa1396400-mRNA-1
tig0022022	CDS	71047	71107	71047	ID=Msa1396400-mRNA-1:cds;Parent=Msa1396400-mRNA-1
tig0022022	CDS	71212	71278	71212	ID=Msa1396400-mRNA-1:cds;Parent=Msa1396400-mRNA-1
tig0022022	CDS	72800	73066	72800	ID=Msa1396400-mRNA-1:cds;Parent=Msa1396400-mRNA-1
tig0022022	CDS	73205	73272	73205	ID=Msa1396400-mRNA-1:cds;Parent=Msa1396400-mRNA-1
tig0022022	CDS	73381	73487	73381	ID=Msa1396400-mRNA-1:cds;Parent=Msa1396400-mRNA-1
tig0022022	CDS	73562	73602	73562	ID=Msa1396400-mRNA-1:cds;Parent=Msa1396400-mRNA-1
tig0022022	CDS	73708	73856	73708	ID=Msa1396400-mRNA-1:cds;Parent=Msa1396400-mRNA-1
tig0022022	CDS	74051	74404	74051	ID=Msa1396400-mRNA-1:cds;Parent=Msa1396400-mRNA-1
tig0022022	CDS	75098	75193	75098	ID=Msa1396400-mRNA-1:cds;Parent=Msa1396400-mRNA-1
tig0022022	three_prime_UTR	75194	75481	75194	ID=Msa1396400-mRNA-1:three_prime_utr;Parent=Msa1396400-mRNA-1

Gene Sequence

>Msa1396400

ATGACGAGAAAAACCCCAAAATCAAGGGGAAGGAAGAAGGCACCACTCGGTGCGAGTAACACAAGACAGTTACAGCTTCAGCTCAGCAAATTCTCTTTCAAACAATACAAACCGTTACCAACAACGACTATAACTTCTTCCGCCTTCCAGAACCTTCTCGATTCAGAGACAAATCAAAATCCTTCCATTAGGGTTACCAATGATGCTACCGATGCTGATTCAAGAGATGGCATTCTTAGTCAAGATTTCTTCTGTACTCCAGATTACATAACTCCGGATAATCAGCGGATTTTGAACGGTATCGATTTCAATAAGGATAATAGCCCTTGCCCTAAATCCCCGGAGAAATTGAATACTACTAAAAGCAAAAGATGTCGACTGGATGGTGTCTCGGCAAACCCTCTTAGCCCTACCTTTTCTGGTGACCATCAACAAGTTGTGGAACTTGGTAAGGACTCTGTTGCAGAGGAAGTAGCTGTTGATATAACGGTAGCTGCTGGTAAGCCGAAGGCCCAGAACTATGTGTCACATTCTGCAGCTACATTGCGTTGCAGGGCTATACCTCCTCCTTGCTTTAGTAACCCCTATTTAAAGGACCTTTCCCAAAAGGAGACAGATCCCTATGCAAACCAAAGATCCAAATGTGCAGGTTTCTTCCCTAAATTTACAGCTTATGATGGGCTCTCACGCTATCGCACTGACTTTCATGAGATTGAGCAAATTGGTCGAGGGTACTTTAGTTGTGTTTTTAAAGTATTAAAGCGAATAGATGGCTGCTTGTATGCTGTGAAACGCAGTACACAGTTACAGCTGGATACAGAAAGGAGAAAAGCTTTGATGGAGGTTCAAGCTCTGGCAGCATTGGGTTACCATGAGAACATTGTGGGCTATTATACTTCATGGTTTGAAAACGAACAACTGTACATTCAGATGGAGATATGTGATCATAGCTTATCTGTAAACAAAGGCTCCGAGTTATTAGCAGAAGGACAAGTGTTAGAAGTTTTATATCAAGTTGCTAATGCACTGCGATTTATACACGACAAGGGAATAGCTCATCTTGATCTTAAACCTGATAATATTTATGTAAAAAATGGTGTCTATAAGCTTGGTGATTTTGGATGTGCAACTCTTACTGATAGTAGCCTTCCGATTGAGGAAGGAGATGCACGTTATATGCCCCAGGAAATCCTTAATGAGAATTATGACCACCTTGACAAGGTTGATATTTTCTCTTTGGGAGTTTCTATTTATCAACTCATCCGAAAATCACCTATGCCAGAATCAGGATGTCATTTTTTGAATCTTAAAGAAGGCAAGTTGCCACTCCTGCCTGGTCATTCATTGCAATTTCAAAACTTGCTCAAGGCCATGATCGATCCTGATCCGGTTAAGAGGCCTTCTGCCAGAGAGTTGGTAGAGAACCCAATTTTCGACAGGGCCTTAAGAACAGCGAAAAATTGA

Protein sequence

>Msa1396400

MTRKTPKSRGRKKAPLGASNTRQLQLQLSKFSFKQYKPLPTTTITSSAFQNLLDSETNQNPSIRVTNDATDADSRDGILSQDFFCTPDYITPDNQRILNGIDFNKDNSPCPKSPEKLNTTKSKRCRLDGVSANPLSPTFSGDHQQVVELGKDSVAEEVAVDITVAAGKPKAQNYVSHSAATLRCRAIPPPCFSNPYLKDLSQKETDPYANQRSKCAGFFPKFTAYDGLSRYRTDFHEIEQIGRGYFSCVFKVLKRIDGCLYAVKRSTQLQLDTERRKALMEVQALAALGYHENIVGYYTSWFENEQLYIQMEICDHSLSVNKGSELLAEGQVLEVLYQVANALRFIHDKGIAHLDLKPDNIYVKNGVYKLGDFGCATLTDSSLPIEEGDARYMPQEILNENYDHLDKVDIFSLGVSIYQLIRKSPMPESGCHFLNLKEGKLPLLPGHSLQFQNLLKAMIDPDPVKRPSARELVENPIFDRALRTAKN*