Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0118380 | XP_024631146.1 | 47.368 | 190 | 90 | 1 | 6 | 185 | 23 | 212 | 3.69e-45 | 158 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0118380 | A0A396JLY5 | 47.368 | 190 | 90 | 1 | 6 | 185 | 26 | 215 | 2.29e-45 | 158 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002930 | Msa0118380 | 0.807405 | 5.136163e-50 | -8.615850e-47 |
| Msa0022640 | Msa0118380 | 0.854870 | 9.226570e-62 | -8.615850e-47 |
| Msa0033340 | Msa0118380 | 0.805311 | 1.419012e-49 | -8.615850e-47 |
| Msa0036430 | Msa0118380 | 0.836498 | 9.033125e-57 | -8.615850e-47 |
| Msa0053110 | Msa0118380 | 0.814331 | 1.626815e-51 | -8.615850e-47 |
| Msa0072810 | Msa0118380 | 0.811594 | 6.474981e-51 | -8.615850e-47 |
| Msa0084500 | Msa0118380 | 0.816042 | 6.780345e-52 | -8.615850e-47 |
| Msa0098440 | Msa0118380 | 0.805606 | 1.230951e-49 | -8.615850e-47 |
| Msa0103000 | Msa0118380 | 0.825890 | 3.663350e-54 | -8.615850e-47 |
| Msa0106790 | Msa0118380 | 0.851469 | 8.697371e-61 | -8.615850e-47 |
| Msa0110970 | Msa0118380 | 0.806038 | 9.988877e-50 | -8.615850e-47 |
| Msa0118380 | Msa0118400 | 0.899524 | 1.819328e-77 | -8.615850e-47 |
| Msa0118380 | Msa0130570 | 0.824432 | 8.097630e-54 | -8.615850e-47 |
| Msa0118380 | Msa0147560 | 0.841001 | 6.190406e-58 | -8.615850e-47 |
| Msa0118380 | Msa0153420 | 0.806339 | 8.631101e-50 | -8.615850e-47 |
| Msa0118380 | Msa0161680 | 0.861428 | 1.032934e-63 | -8.615850e-47 |
| Msa0118380 | Msa0171020 | 0.815867 | 7.418107e-52 | -8.615850e-47 |
| Msa0118380 | Msa0176470 | 0.809865 | 1.531597e-50 | -8.615850e-47 |
| Msa0118380 | Msa0196150 | 0.801555 | 8.520440e-49 | -8.615850e-47 |
| Msa0118380 | Msa0203030 | 0.824335 | 8.533069e-54 | -8.615850e-47 |
| Msa0118380 | Msa0219190 | 0.800043 | 1.734434e-48 | -8.615850e-47 |
| Msa0118380 | Msa0280490 | 0.804143 | 2.488441e-49 | -8.615850e-47 |
| Msa0118380 | Msa0322830 | 0.807073 | 6.039693e-50 | -8.615850e-47 |
| Msa0118380 | Msa0337850 | 0.824946 | 6.126079e-54 | -8.615850e-47 |
| Msa0118380 | Msa0362780 | 0.803891 | 2.808080e-49 | -8.615850e-47 |
| Msa0118380 | Msa0378500 | 0.825262 | 5.159764e-54 | -8.615850e-47 |
| Msa0118380 | Msa0419970 | 0.800257 | 1.569193e-48 | -8.615850e-47 |
| Msa0118380 | Msa0436460 | 0.818099 | 2.338902e-52 | -8.615850e-47 |
| Msa0118380 | Msa0436500 | 0.847496 | 1.114493e-59 | -8.615850e-47 |
| Msa0118380 | Msa0455160 | 0.808390 | 3.171012e-50 | -8.615850e-47 |
| Msa0118380 | Msa0484690 | 0.803762 | 2.986708e-49 | -8.615850e-47 |
| Msa0118380 | Msa0584130 | 0.818466 | 1.931771e-52 | -8.615850e-47 |
| Msa0118380 | Msa0682530 | 0.828991 | 6.605892e-55 | -8.615850e-47 |
| Msa0118380 | Msa0734660 | 0.846530 | 2.050305e-59 | -8.615850e-47 |
| Msa0118380 | Msa0747780 | 0.810300 | 1.234224e-50 | -8.615850e-47 |
| Msa0118380 | Msa0760890 | 0.801140 | 1.035972e-48 | -8.615850e-47 |
| Msa0118380 | Msa0776220 | 0.849178 | 3.820876e-60 | -8.615850e-47 |
| Msa0118380 | Msa0813670 | 0.832119 | 1.133844e-55 | -8.615850e-47 |
| Msa0118380 | Msa0853540 | 0.802164 | 6.389129e-49 | -8.615850e-47 |
| Msa0118380 | Msa0854120 | 0.818775 | 1.643890e-52 | -8.615850e-47 |
| Msa0118380 | Msa0885060 | 0.859885 | 3.034253e-63 | -8.615850e-47 |
| Msa0118380 | Msa0946320 | 0.811402 | 7.129742e-51 | -8.615850e-47 |
| Msa0118380 | Msa0951710 | 0.800402 | 1.465520e-48 | -8.615850e-47 |
| Msa0118380 | Msa1010110 | 0.819708 | 1.007980e-52 | -8.615850e-47 |
| Msa0118380 | Msa1034680 | 0.812716 | 3.686319e-51 | -8.615850e-47 |
| Msa0118380 | Msa1048330 | 0.803085 | 4.125074e-49 | -8.615850e-47 |
| Msa0118380 | Msa1055280 | 0.800231 | 1.587891e-48 | -8.615850e-47 |
| Msa0118380 | Msa1135690 | 0.826451 | 2.692992e-54 | -8.615850e-47 |
| Msa0118380 | Msa1176160 | 0.805009 | 1.641521e-49 | -8.615850e-47 |
| Msa0118380 | Msa1196080 | 0.828976 | 6.663215e-55 | -8.615850e-47 |
| Msa0118380 | Msa1248040 | 0.843472 | 1.372334e-58 | -8.615850e-47 |
| Msa0118380 | Msa1374440 | 0.806757 | 7.045681e-50 | -8.615850e-47 |
| Msa0118380 | Msa1379990 | 0.824974 | 6.035187e-54 | -8.615850e-47 |
| Msa0118380 | Msa1411070 | 0.807966 | 3.903764e-50 | -8.615850e-47 |
| Msa0118380 | Msa1420060 | 0.807643 | 4.573278e-50 | -8.615850e-47 |
| Msa0118380 | Msa1424690 | 0.831989 | 1.220820e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0118380 | MtrunA17_Chr1g0175821 | 47.368 | 190 | 90 | 1 | 6 | 185 | 26 | 215 | 4.41e-49 | 158 |
| Msa0118380 | MtrunA17_Chr1g0175811 | 39.819 | 221 | 83 | 6 | 1 | 186 | 1 | 206 | 1.17e-34 | 121 |
| Msa0118380 | MtrunA17_Chr1g0175791 | 47.205 | 161 | 57 | 3 | 8 | 141 | 67 | 226 | 3.04e-30 | 110 |
| Msa0118380 | MtrunA17_Chr5g0420601 | 34.826 | 201 | 93 | 5 | 21 | 186 | 85 | 282 | 3.45e-21 | 87.8 |
| Msa0118380 | MtrunA17_Chr1g0175781 | 32.353 | 204 | 97 | 5 | 21 | 186 | 90 | 290 | 4.58e-19 | 82.4 |
| Msa0118380 | MtrunA17_Chr1g0175751 | 31.373 | 204 | 99 | 4 | 21 | 186 | 119 | 319 | 1.26e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAACTTGGTCAAGGTTTA+AGG | 0.142695 | 1_3:-41060900 | None:intergenic |
| CTTGTAAACAATTCCTTCTT+TGG | 0.221022 | 1_3:-41060758 | None:intergenic |
| CTTCGAAAAGAGTGGATTAA+TGG | 0.276341 | 1_3:+41060681 | Msa0118380:CDS |
| GGAGCAAAAGGTTTGTGATT+TGG | 0.304158 | 1_3:+41060552 | Msa0118380:CDS |
| CTTGGTCAAGGTTTAAGGTT+AGG | 0.388940 | 1_3:-41060895 | None:intergenic |
| ATGGCTACTCTACATCAATC+TGG | 0.390287 | 1_3:+41060413 | Msa0118380:CDS |
| GCAAGCAACACCCGTTCAAT+TGG | 0.411385 | 1_3:+41060510 | Msa0118380:CDS |
| TGCTCGTTGTATGGAGCAAA+AGG | 0.418843 | 1_3:+41060540 | Msa0118380:CDS |
| GGAAAATTGCACACCAAAGA+AGG | 0.418990 | 1_3:+41060745 | Msa0118380:CDS |
| GGTCTCGATTGCTCGTTGTA+TGG | 0.434380 | 1_3:+41060531 | Msa0118380:CDS |
| AGAAGGAATTGTTTACAAGT+TGG | 0.457133 | 1_3:+41060762 | Msa0118380:CDS |
| GTGATTTGGAAAAGAGCTGC+AGG | 0.479576 | 1_3:+41060566 | Msa0118380:CDS |
| AGGAATTGTTTACAAGTTGG+AGG | 0.487776 | 1_3:+41060765 | Msa0118380:CDS |
| AAAGGATCATGAGGCATATG+AGG | 0.490646 | 1_3:+41060456 | Msa0118380:CDS |
| GAGTGGATTAATGGAAAGAG+AGG | 0.504421 | 1_3:+41060690 | Msa0118380:CDS |
| CTGAATCTGAGAATAACATA+GGG | 0.510118 | 1_3:+41060629 | Msa0118380:CDS |
| TCTGAATCTGAGAATAACAT+AGG | 0.510558 | 1_3:+41060628 | Msa0118380:CDS |
| TCATGAGGCATATGAGGAGT+GGG | 0.527499 | 1_3:+41060462 | Msa0118380:CDS |
| GCAATCGAGACCAATTGAAC+GGG | 0.527802 | 1_3:-41060520 | None:intergenic |
| GTAGAGTAGCCATTCTCAGC+TGG | 0.533250 | 1_3:-41060403 | None:intergenic |
| AAGACTACACCAGCTGAGAA+TGG | 0.552919 | 1_3:+41060394 | Msa0118380:CDS |
| GCACAATGTTGGCTTTAATG+AGG | 0.553089 | 1_3:+41060846 | Msa0118380:CDS |
| TGGCTACTCTACATCAATCT+GGG | 0.557094 | 1_3:+41060414 | Msa0118380:CDS |
| ATTGTAGTGCAGCACAATGT+TGG | 0.569055 | 1_3:+41060835 | Msa0118380:CDS |
| GAATTAATCTTCGAAAAGAG+TGG | 0.570774 | 1_3:+41060673 | Msa0118380:CDS |
| ATCATGAGGCATATGAGGAG+TGG | 0.581486 | 1_3:+41060461 | Msa0118380:CDS |
| AAGAGCTGCAGGGCTCACAT+TGG | 0.582753 | 1_3:+41060577 | Msa0118380:CDS |
| AGAATGGATCCCAAGAGATG+TGG | 0.586826 | 1_3:+41060349 | None:intergenic |
| CGGGTGTTGCTTGCAAAACA+CGG | 0.601763 | 1_3:-41060501 | None:intergenic |
| TATCACAGAAAAGGATCATG+AGG | 0.608121 | 1_3:+41060447 | Msa0118380:CDS |
| AGCAATCGAGACCAATTGAA+CGG | 0.624783 | 1_3:-41060521 | None:intergenic |
| TGATTTGGAAAAGAGCTGCA+GGG | 0.627589 | 1_3:+41060567 | Msa0118380:CDS |
| CATGAGGCATATGAGGAGTG+GGG | 0.686751 | 1_3:+41060463 | Msa0118380:CDS |
| TGAATCTGAGAATAACATAG+GGG | 0.713948 | 1_3:+41060630 | Msa0118380:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGACAATTTTATCACAGAAA+AGG | + | chr1_3:41060438-41060457 | Msa0118380:CDS | 25.0% |
| !! | AATGAAAACTCCAAGATTTT+AGG | + | chr1_3:41060724-41060743 | Msa0118380:CDS | 25.0% |
| TCTGAATCTGAGAATAACAT+AGG | + | chr1_3:41060628-41060647 | Msa0118380:CDS | 30.0% | |
| CTGAATCTGAGAATAACATA+GGG | + | chr1_3:41060629-41060648 | Msa0118380:CDS | 30.0% | |
| TGAATCTGAGAATAACATAG+GGG | + | chr1_3:41060630-41060649 | Msa0118380:CDS | 30.0% | |
| GAATTAATCTTCGAAAAGAG+TGG | + | chr1_3:41060673-41060692 | Msa0118380:CDS | 30.0% | |
| ! | GTGCAATTTTCCTAAAATCT+TGG | - | chr1_3:41060737-41060756 | None:intergenic | 30.0% |
| CTTGTAAACAATTCCTTCTT+TGG | - | chr1_3:41060761-41060780 | None:intergenic | 30.0% | |
| AGAAGGAATTGTTTACAAGT+TGG | + | chr1_3:41060762-41060781 | Msa0118380:CDS | 30.0% | |
| TATCACAGAAAAGGATCATG+AGG | + | chr1_3:41060447-41060466 | Msa0118380:CDS | 35.0% | |
| !! | CTTCGAAAAGAGTGGATTAA+TGG | + | chr1_3:41060681-41060700 | Msa0118380:CDS | 35.0% |
| ! | AGGAATTGTTTACAAGTTGG+AGG | + | chr1_3:41060765-41060784 | Msa0118380:CDS | 35.0% |
| ! | CTACGTTTTCCACATCTCTT+GGG | - | chr1_3:41060361-41060380 | None:intergenic | 40.0% |
| ATGGCTACTCTACATCAATC+TGG | + | chr1_3:41060413-41060432 | Msa0118380:CDS | 40.0% | |
| TGGCTACTCTACATCAATCT+GGG | + | chr1_3:41060414-41060433 | Msa0118380:CDS | 40.0% | |
| AAAGGATCATGAGGCATATG+AGG | + | chr1_3:41060456-41060475 | Msa0118380:CDS | 40.0% | |
| AGCAATCGAGACCAATTGAA+CGG | - | chr1_3:41060524-41060543 | None:intergenic | 40.0% | |
| GGAGCAAAAGGTTTGTGATT+TGG | + | chr1_3:41060552-41060571 | Msa0118380:CDS | 40.0% | |
| ! | TGATTTGGAAAAGAGCTGCA+GGG | + | chr1_3:41060567-41060586 | Msa0118380:CDS | 40.0% |
| !! | GAGTGGATTAATGGAAAGAG+AGG | + | chr1_3:41060690-41060709 | Msa0118380:CDS | 40.0% |
| GGAAAATTGCACACCAAAGA+AGG | + | chr1_3:41060745-41060764 | Msa0118380:CDS | 40.0% | |
| ATTGTAGTGCAGCACAATGT+TGG | + | chr1_3:41060835-41060854 | Msa0118380:CDS | 40.0% | |
| !! | GCACAATGTTGGCTTTAATG+AGG | + | chr1_3:41060846-41060865 | Msa0118380:CDS | 40.0% |
| ! | GCTACGTTTTCCACATCTCT+TGG | - | chr1_3:41060362-41060381 | None:intergenic | 45.0% |
| AAGACTACACCAGCTGAGAA+TGG | + | chr1_3:41060394-41060413 | Msa0118380:CDS | 45.0% | |
| ATCATGAGGCATATGAGGAG+TGG | + | chr1_3:41060461-41060480 | Msa0118380:CDS | 45.0% | |
| TCATGAGGCATATGAGGAGT+GGG | + | chr1_3:41060462-41060481 | Msa0118380:CDS | 45.0% | |
| GCAATCGAGACCAATTGAAC+GGG | - | chr1_3:41060523-41060542 | None:intergenic | 45.0% | |
| TGCTCGTTGTATGGAGCAAA+AGG | + | chr1_3:41060540-41060559 | Msa0118380:CDS | 45.0% | |
| ! | GTGATTTGGAAAAGAGCTGC+AGG | + | chr1_3:41060566-41060585 | Msa0118380:CDS | 45.0% |
| GTAGAGTAGCCATTCTCAGC+TGG | - | chr1_3:41060406-41060425 | None:intergenic | 50.0% | |
| CATGAGGCATATGAGGAGTG+GGG | + | chr1_3:41060463-41060482 | Msa0118380:CDS | 50.0% | |
| !! | CGGGTGTTGCTTGCAAAACA+CGG | - | chr1_3:41060504-41060523 | None:intergenic | 50.0% |
| GCAAGCAACACCCGTTCAAT+TGG | + | chr1_3:41060510-41060529 | Msa0118380:CDS | 50.0% | |
| GGTCTCGATTGCTCGTTGTA+TGG | + | chr1_3:41060531-41060550 | Msa0118380:CDS | 50.0% | |
| AAGAGCTGCAGGGCTCACAT+TGG | + | chr1_3:41060577-41060596 | Msa0118380:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_3 | gene | 41060352 | 41060912 | 41060352 | ID=Msa0118380;Name=Msa0118380 |
| chr1_3 | mRNA | 41060352 | 41060912 | 41060352 | ID=Msa0118380-mRNA-1;Parent=Msa0118380;Name=Msa0118380-mRNA-1;_AED=0.33;_eAED=0.33;_QI=0|-1|0|1|-1|1|1|0|186 |
| chr1_3 | exon | 41060352 | 41060912 | 41060352 | ID=Msa0118380-mRNA-1:exon:12461;Parent=Msa0118380-mRNA-1 |
| chr1_3 | CDS | 41060352 | 41060912 | 41060352 | ID=Msa0118380-mRNA-1:cds;Parent=Msa0118380-mRNA-1 |
| Gene Sequence |
| Protein sequence |
