AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0173750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0173750 MtrunA17_Chr1g0193961 90.538 465 31 2 1 452 7 471 0.0 834
Msa0173750 MtrunA17_Chr7g0270821 79.912 453 83 2 1 445 7 459 0.0 754
Msa0173750 MtrunA17_Chr2g0295241 54.545 396 170 3 33 418 33 428 6.47e-156 449
Msa0173750 MtrunA17_Chr8g0387391 51.559 417 193 4 19 427 34 449 3.49e-155 448
Msa0173750 MtrunA17_Chr4g0067811 54.660 397 170 3 33 419 36 432 2.68e-154 445
Msa0173750 MtrunA17_Chr3g0095221 54.897 388 171 1 38 421 55 442 1.21e-152 441
Msa0173750 MtrunA17_Chr1g0202651 54.897 388 171 2 38 421 63 450 8.21e-151 437
Msa0173750 MtrunA17_Chr8g0348491 52.019 421 187 4 33 438 51 471 2.41e-148 431
Msa0173750 MtrunA17_Chr7g0255311 54.731 391 170 3 38 421 71 461 4.21e-148 431
Msa0173750 MtrunA17_Chr7g0248451 52.645 397 183 3 38 430 63 458 5.82e-148 430
Msa0173750 MtrunA17_Chr3g0130251 53.181 393 182 2 29 419 75 467 8.20e-142 414
Msa0173750 MtrunA17_Chr1g0202641 53.728 389 176 2 37 421 62 450 3.67e-141 428
Msa0173750 MtrunA17_Chr1g0202641 53.608 388 176 2 38 421 563 950 4.90e-138 420
Msa0173750 MtrunA17_Chr5g0442221 55.297 387 167 2 38 418 52 438 7.56e-141 411
Msa0173750 MtrunA17_Chr4g0070281 47.126 435 210 7 1 420 41 470 6.25e-136 400
Msa0173750 MtrunA17_Chr1g0202621 50.386 389 183 3 37 421 55 437 9.42e-136 398
Msa0173750 MtrunA17_Chr2g0301091 48.309 414 199 7 19 421 46 455 4.07e-133 392
Msa0173750 MtrunA17_Chr2g0319881 85.549 173 18 1 1 166 7 179 2.32e-97 290
Msa0173750 MtrunA17_Chr5g0418411 37.004 227 122 6 123 344 112 322 3.85e-27 112
Msa0173750 MtrunA17_Chr2g0319871 70.312 64 19 0 172 235 1 64 2.67e-23 92.8
Msa0173750 MtrunA17_Chr3g0121891 30.769 234 140 5 137 366 126 341 3.20e-22 98.2
Msa0173750 MtrunA17_Chr8g0337571 32.653 196 112 4 136 327 121 300 1.63e-21 95.9
Msa0173750 MtrunA17_Chr8g0365901 28.955 335 197 12 39 363 74 377 2.93e-21 95.9
Msa0173750 MtrunA17_Chr3g0100091 35.149 202 107 6 133 328 77 260 3.11e-21 94.7
Msa0173750 MtrunA17_Chr7g0258111 26.899 316 184 8 32 333 50 332 8.99e-21 94.7
Msa0173750 MtrunA17_Chr4g0043661 28.763 299 173 8 132 413 115 390 1.20e-20 93.6
Msa0173750 MtrunA17_Chr6g0449961 33.014 209 121 5 133 337 133 326 1.43e-20 93.6
Msa0173750 MtrunA17_Chr3g0121881 29.452 292 173 8 132 412 125 394 2.65e-20 92.4
Msa0173750 MtrunA17_Chr5g0413141 28.481 316 187 10 143 433 156 457 3.17e-19 89.7
Msa0173750 MtrunA17_Chr7g0230891 26.097 433 249 17 7 415 14 399 3.51e-19 89.4
Msa0173750 MtrunA17_Chr1g0150291 25.467 428 263 13 1 414 22 407 1.12e-18 87.8
Msa0173750 MtrunA17_Chr6g0468871 53.247 77 35 1 223 298 22 98 1.51e-18 80.5
Msa0173750 MtrunA17_Chr8g0336481 29.787 235 127 7 136 361 43 248 2.81e-18 84.7
Msa0173750 MtrunA17_Chr4g0020771 29.614 233 138 7 143 363 168 386 9.36e-18 85.1
Msa0173750 MtrunA17_Chr8g0337581 31.628 215 125 6 135 344 121 318 1.86e-17 84.0
Msa0173750 MtrunA17_Chr2g0332971 30.392 204 122 5 130 325 115 306 2.88e-17 83.6
Msa0173750 MtrunA17_Chr5g0413411 25.595 336 181 10 39 337 130 433 3.72e-17 84.0
Msa0173750 MtrunA17_Chr3g0089021 29.259 270 158 7 104 367 138 380 3.79e-17 83.6
Msa0173750 MtrunA17_Chr2g0332801 31.466 232 133 7 121 341 136 352 4.29e-17 83.2
Msa0173750 MtrunA17_Chr4g0052621 28.228 333 191 11 20 337 59 358 1.16e-16 82.0
Msa0173750 MtrunA17_Chr7g0270361 26.722 363 199 13 29 367 66 385 1.49e-16 81.6
Msa0173750 MtrunA17_Chr8g0337601 32.941 170 98 3 179 344 12 169 4.67e-16 77.4
Msa0173750 MtrunA17_Chr1g0150301 24.057 424 274 12 1 414 4 389 6.48e-16 79.3
Msa0173750 MtrunA17_Chr6g0461181 23.853 436 253 15 21 405 15 422 8.05e-16 79.3
Msa0173750 MtrunA17_Chr4g0018151 25.761 427 262 13 1 412 49 435 8.87e-16 79.3
Msa0173750 MtrunA17_Chr4g0043431 27.859 341 204 13 40 371 45 352 9.08e-16 79.0
Msa0173750 MtrunA17_Chr8g0365891 28.014 282 136 8 92 337 170 420 2.36e-15 78.2
Msa0173750 MtrunA17_Chr1g0194231 27.077 325 189 8 31 337 82 376 3.65e-15 77.4
Msa0173750 MtrunA17_Chr2g0303941 32.212 208 118 6 143 342 122 314 7.56e-15 75.9
Msa0173750 MtrunA17_Chr7g0228411 27.859 341 189 12 28 339 38 350 1.74e-13 72.0
Msa0173750 MtrunA17_Chr1g0173451 27.551 294 172 11 143 420 172 440 4.99e-13 70.9
Msa0173750 MtrunA17_Chr8g0337511 31.737 167 102 3 176 340 12 168 8.72e-13 68.2
Msa0173750 MtrunA17_Chr2g0293681 28.704 216 129 5 135 337 157 360 2.32e-12 68.6
Msa0173750 MtrunA17_Chr7g0257701 28.718 195 119 4 137 327 191 369 2.47e-12 68.6
Msa0173750 MtrunA17_Chr1g0195221 29.108 213 131 5 135 339 124 324 2.58e-12 68.2
Msa0173750 MtrunA17_Chr4g0067631 24.752 303 182 11 39 327 61 331 5.47e-12 67.4
Msa0173750 MtrunA17_Chr1g0149241 23.333 330 218 8 1 327 5 302 7.67e-12 66.6
Msa0173750 MtrunA17_Chr6g0468861 43.750 64 35 1 333 395 12 75 5.11e-11 58.5
Msa0173750 MtrunA17_Chr2g0291841 26.174 298 188 12 132 415 82 361 6.24e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0173750 AT3G62110.1 67.238 467 136 8 1 452 7 471 0.0 625
Msa0173750 AT3G62110.2 67.238 467 136 8 1 452 7 471 0.0 625
Msa0173750 AT4G23820.1 53.052 426 191 2 2 418 6 431 6.59e-156 449
Msa0173750 AT4G23500.1 52.826 407 187 3 19 421 58 463 1.44e-154 447
Msa0173750 AT4G33440.1 55.440 386 170 2 38 421 80 465 5.43e-150 435
Msa0173750 AT3G61490.2 53.886 386 174 2 38 419 53 438 6.97e-150 435
Msa0173750 AT3G61490.3 53.886 386 174 2 38 419 53 438 6.97e-150 435
Msa0173750 AT3G61490.1 53.886 386 174 2 38 419 53 438 6.97e-150 435
Msa0173750 AT3G61490.5 53.886 386 174 2 38 419 68 453 1.25e-149 435
Msa0173750 AT3G61490.4 53.886 386 174 2 38 419 166 551 2.82e-149 437
Msa0173750 AT3G16850.1 49.770 434 204 4 24 448 27 455 4.44e-146 425
Msa0173750 AT5G41870.1 54.026 385 175 2 37 419 52 436 1.96e-145 423
Msa0173750 AT3G48950.1 49.640 417 198 4 33 437 41 457 4.79e-145 422
Msa0173750 AT5G49215.2 51.508 398 181 3 33 418 37 434 2.25e-140 410
Msa0173750 AT5G49215.1 51.508 398 181 3 33 418 39 436 2.93e-140 409
Msa0173750 AT2G23900.1 46.697 439 215 6 5 424 17 455 1.37e-136 401
Msa0173750 AT3G06770.2 50.000 398 189 3 33 420 39 436 3.56e-135 397
Msa0173750 AT3G06770.5 50.000 398 189 3 33 420 40 437 3.72e-135 396
Msa0173750 AT1G19170.1 47.826 391 192 6 38 419 95 482 3.36e-123 368
Msa0173750 AT3G42950.1 45.501 389 207 4 37 420 73 461 7.99e-123 366
Msa0173750 AT3G06770.1 48.907 366 184 2 58 420 2 367 4.67e-122 360
Msa0173750 AT3G06770.3 48.907 366 184 2 58 420 2 367 4.67e-122 360
Msa0173750 AT3G06770.4 45.115 348 183 3 81 420 2 349 4.46e-101 306
Msa0173750 AT3G57790.2 28.391 317 184 6 32 333 37 325 1.74e-28 117
Msa0173750 AT3G57790.1 28.391 317 184 6 32 333 37 325 1.74e-28 117
Msa0173750 AT3G15720.3 30.056 356 197 13 28 371 21 336 3.49e-26 110
Msa0173750 AT3G15720.1 30.056 356 197 13 28 371 22 337 3.52e-26 110
Msa0173750 AT3G15720.2 30.337 356 193 14 28 371 21 333 1.27e-24 106
Msa0173750 AT3G15720.4 32.794 247 140 7 133 371 45 273 1.33e-24 105
Msa0173750 AT5G14650.1 28.333 300 183 8 32 327 53 324 1.65e-22 99.8
Msa0173750 AT1G70500.1 28.035 346 208 11 28 363 65 379 6.16e-21 95.5
Msa0173750 AT4G32380.1 33.990 203 113 5 133 331 118 303 6.65e-21 95.1
Msa0173750 AT5G17200.1 28.763 299 175 8 31 325 40 304 2.41e-20 93.2
Msa0173750 AT3G26610.1 31.553 206 119 5 142 337 163 356 7.73e-20 92.0
Msa0173750 AT5G27530.1 26.316 380 227 12 39 407 62 399 9.12e-20 92.0
Msa0173750 AT1G60590.1 23.929 397 221 12 39 391 132 491 1.64e-19 91.7
Msa0173750 AT4G35670.2 35.354 198 108 5 133 326 8 189 1.72e-19 89.0
Msa0173750 AT4G35670.1 34.951 206 114 5 125 326 107 296 3.08e-19 89.7
Msa0173750 AT1G56710.1 29.052 327 188 9 29 339 47 345 8.18e-19 89.0
Msa0173750 AT1G23460.2 25.797 345 217 8 28 363 41 355 8.91e-19 88.6
Msa0173750 AT1G23460.1 26.361 349 210 10 28 363 65 379 1.15e-18 88.6
Msa0173750 AT1G10640.1 25.000 396 215 12 39 391 128 484 1.16e-18 89.0
Msa0173750 AT5G14650.2 26.882 279 172 8 32 306 53 303 2.67e-18 85.9
Msa0173750 AT2G15460.1 26.121 379 236 10 5 371 6 352 6.36e-18 85.9
Msa0173750 AT3G57510.1 27.027 370 210 14 39 397 77 397 7.65e-18 85.9
Msa0173750 AT2G15470.1 26.684 386 225 14 5 371 6 352 1.57e-17 84.7
Msa0173750 AT5G44840.1 32.796 186 106 4 143 324 124 294 2.63e-17 84.0
Msa0173750 AT2G15450.1 25.722 381 235 11 5 371 6 352 9.13e-17 82.4
Msa0173750 AT1G80170.1 25.220 341 219 8 39 371 62 374 2.67e-16 81.3
Msa0173750 AT1G17150.1 24.672 381 237 11 4 371 8 351 3.45e-16 80.5
Msa0173750 AT2G26620.1 26.230 366 223 10 18 371 20 350 3.95e-16 80.5
Msa0173750 AT2G43880.2 28.896 308 173 10 31 328 32 303 4.29e-16 79.7
Msa0173750 AT2G41850.1 29.866 298 171 9 39 327 77 345 5.06e-16 80.5
Msa0173750 AT2G43880.1 26.131 398 245 13 31 415 32 393 5.13e-16 80.1
Msa0173750 AT5G44830.1 31.720 186 98 5 143 324 75 235 8.15e-16 79.0
Msa0173750 AT4G32375.1 32.524 206 114 7 132 331 18 204 9.34e-16 79.3
Msa0173750 AT1G02460.1 26.899 316 188 7 29 327 88 377 1.96e-15 79.0
Msa0173750 AT4G32380.2 31.553 206 119 6 133 333 118 306 3.00e-15 77.8
Msa0173750 AT4G13760.1 24.422 389 239 13 1 371 1 352 3.51e-15 77.4
Msa0173750 AT3G07970.1 29.134 254 151 7 123 367 148 381 3.55e-15 77.8
Msa0173750 AT1G48100.1 25.915 328 201 9 29 342 79 378 3.66e-15 77.8
Msa0173750 AT4G32370.1 28.740 254 152 9 111 356 110 342 9.55e-14 72.8
Msa0173750 AT1G78400.1 25.063 399 253 14 31 415 36 402 1.02e-13 73.2
Msa0173750 AT1G02790.1 24.425 348 216 9 29 368 51 359 1.18e-13 73.2
Msa0173750 AT1G80140.1 30.049 203 122 5 143 341 70 256 1.24e-13 72.4
Msa0173750 AT4G01890.1 25.161 310 191 8 29 322 64 348 1.75e-13 72.8
Msa0173750 AT1G80170.2 26.449 276 167 8 39 306 62 309 2.91e-13 71.2
Msa0173750 AT3G14040.1 26.059 307 203 9 124 418 146 440 3.99e-13 71.6
Msa0173750 AT2G40310.1 23.760 383 249 12 1 371 1 352 5.91e-13 70.9
Msa0173750 AT1G43080.1 25.134 374 239 11 1 363 1 344 7.81e-13 70.5
Msa0173750 AT2G43870.1 27.027 222 143 4 128 348 104 307 1.22e-12 69.7
Msa0173750 AT1G65570.1 27.609 297 182 10 131 415 121 396 1.58e-12 69.3
Msa0173750 AT1G43100.1 25.333 375 237 12 1 363 1 344 1.75e-12 69.3
Msa0173750 AT3G07850.1 25.407 307 205 9 124 418 145 439 4.01e-12 68.2
Msa0173750 AT1G43090.1 24.866 374 240 11 1 363 1 344 6.71e-12 67.8
Msa0173750 AT1G05650.1 25.000 280 193 7 143 415 122 391 8.06e-12 67.0
Msa0173750 AT5G48140.1 30.412 194 113 7 143 327 113 293 9.86e-12 67.0
Msa0173750 AT2G43890.1 26.408 284 183 10 143 415 123 391 1.15e-11 66.6
Msa0173750 AT4G18180.1 30.366 191 117 5 143 327 137 317 1.78e-11 66.2
Msa0173750 AT3G59850.1 25.237 317 207 7 40 354 33 321 2.33e-11 65.9
Msa0173750 AT3G59850.2 25.430 291 189 6 40 328 77 341 4.27e-11 65.1

Find 132 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCTTGAATTCACCATTT+TGG 0.176069 1_4:-59110053 Msa0173750:CDS
TTTATCACAGGTAGAGTATC+TGG 0.180895 1_4:+59109150 None:intergenic
TTCAAGTATCTTTGAACTTT+TGG 0.194110 1_4:+59108800 None:intergenic
CTTTGTCGGCATACGAATTT+AGG 0.216480 1_4:+59111163 None:intergenic
GTCACAATCCTTGCTCCTTT+TGG 0.216904 1_4:-59109861 Msa0173750:CDS
ACTGACTATTGTTATGATTA+AGG 0.221969 1_4:-59108612 Msa0173750:three_prime_UTR
ATTAGATTCACTGGTTTATA+TGG 0.231131 1_4:-59109198 Msa0173750:CDS
CACCTTTAATACCTTGTATC+AGG 0.254668 1_4:+59109079 None:intergenic
GGATCAATCCCATCTGTATT+TGG 0.262778 1_4:+59109834 None:intergenic
TGGAACTGCTCGGACGTTAA+AGG 0.278579 1_4:-59108997 Msa0173750:CDS
ATACTCTACCTGTGATAAAA+AGG 0.281635 1_4:-59109146 Msa0173750:CDS
CGGCATAGAAGCCTCATTTA+TGG 0.285632 1_4:-59110325 Msa0173750:CDS
CTCACTTCCGATGGCGATTC+CGG 0.289304 1_4:+59109371 None:intergenic
AGAACAAAACCTGCAATATA+CGG 0.290931 1_4:+59110020 None:intergenic
TTGAGTTGCTCACAAGCTTC+CGG 0.312746 1_4:+59108955 None:intergenic
CGAACTTCCTTAATGTTTAT+CGG 0.317616 1_4:+59111273 None:intergenic
TAGATGGTCAGGGGAGTATT+TGG 0.324308 1_4:-59110167 Msa0173750:CDS
AAAAGTGGATGGGATGAATA+TGG 0.325657 1_4:-59109459 Msa0173750:CDS
TTAACTGATGTCATCATAAC+AGG 0.329835 1_4:-59110295 Msa0173750:intron
CTGCAAACCGATAAACATTA+AGG 0.342230 1_4:-59111280 Msa0173750:CDS
ATTGTAAAGTGTCAGTTTAG+TGG 0.353949 1_4:-59108674 Msa0173750:three_prime_UTR
TGAGTTGCTCACAAGCTTCC+GGG 0.361594 1_4:+59108956 None:intergenic
TCATCAGCCATCTCACTTTG+TGG 0.368760 1_4:-59111086 Msa0173750:CDS
GTTGCAGCTCATGGACAGTT+TGG 0.372964 1_4:-59111314 Msa0173750:CDS
CTATTCATCCCGTATATTGC+AGG 0.373700 1_4:-59110029 Msa0173750:intron
GATGAATATGGAATTTCATT+TGG 0.376190 1_4:-59109447 Msa0173750:CDS
GATATTGCTATTAGATTCAC+TGG 0.376562 1_4:-59109207 Msa0173750:CDS
CCAAGGTGATGTTAGAGATA+TGG 0.381148 1_4:+59109238 None:intergenic
ACGGGATGAATAGTCCAAAA+TGG 0.385267 1_4:+59110039 None:intergenic
ATTACTGTCGAGAATGTGAT+AGG 0.387685 1_4:-59109123 Msa0173750:CDS
CATTGATTTGTTGCAGCTCA+TGG 0.393647 1_4:-59111323 Msa0173750:CDS
CAATGGAACTATAGATGGTC+AGG 0.395696 1_4:-59110178 Msa0173750:CDS
CGTTAAAGGATACTCTGAAT+TGG 0.398386 1_4:-59108983 Msa0173750:CDS
GGCCTGATACAAGGTATTAA+AGG 0.401311 1_4:-59109081 Msa0173750:CDS
AGAGTTCGAGTAGTCGAAAC+AGG 0.413361 1_4:-59108876 Msa0173750:CDS
TCCGGCACTCGTTGTCCTCC+CGG 0.414226 1_4:+59109389 None:intergenic
GGCATAGAAGCCTCATTTAT+GGG 0.414586 1_4:-59110324 Msa0173750:CDS
CCTGCTGGCCAGTGGTTAAC+CGG 0.420724 1_4:-59111120 Msa0173750:CDS
CCGGTTAACCACTGGCCAGC+AGG 0.424506 1_4:+59111120 None:intergenic
GGGTTGCTTGGTTGCAGTCT+TGG 0.430839 1_4:-59108855 Msa0173750:CDS
TTTATCAATTCGACTAAATG+AGG 0.438068 1_4:+59110105 None:intergenic
AAGCTTGGCTCCACCTTTGT+CGG 0.447146 1_4:+59111149 None:intergenic
GTCAGTTTAGTGGTAAGTGC+AGG 0.450053 1_4:-59108664 Msa0173750:three_prime_UTR
GGCAACAATGGAACTATAGA+TGG 0.450842 1_4:-59110183 Msa0173750:CDS
TCTGGGTCATATGAGTCATC+TGG 0.458182 1_4:+59109168 None:intergenic
ATAAGAATAAAGACTTCTCC+CGG 0.466538 1_4:-59109285 Msa0173750:CDS
TTAAATTGTGCCCATAAATG+AGG 0.481414 1_4:+59110314 None:intergenic
GATCTAATTTCCATCAAAAG+TGG 0.482686 1_4:-59109474 Msa0173750:CDS
GACAAAGATGCAGTAATACT+TGG 0.490886 1_4:-59111060 Msa0173750:CDS
TGTTACAGAACTCTGAAGAT+TGG 0.490896 1_4:-59110402 Msa0173750:intron
TTACAAGTTAACAGATATCA+TGG 0.491363 1_4:-59108899 Msa0173750:CDS
CTCTGAAGATTGGCCAGTTG+TGG 0.495481 1_4:-59110392 Msa0173750:CDS
GTGGATCCTCTACCATCATA+TGG 0.496479 1_4:-59110373 Msa0173750:CDS
TTATCACAGGTAGAGTATCT+GGG 0.500131 1_4:+59109151 None:intergenic
TGCTTGGTTGCAGTCTTGGT+AGG 0.501300 1_4:-59108851 Msa0173750:exon
CAGCCATCTCACTTTGTGGT+TGG 0.504099 1_4:-59111082 Msa0173750:CDS
TCCTCTACCATCATATGGTA+GGG 0.504397 1_4:-59110368 Msa0173750:CDS
ACCGGGAGGACAACGAGTGC+CGG 0.510929 1_4:-59109390 Msa0173750:CDS
TTTAACATAACCACCCCTCC+CGG 0.511069 1_4:+59109266 None:intergenic
GATGGTAGAGGATCCACAAC+TGG 0.512083 1_4:+59110379 None:intergenic
AATACAGATGGGATTGATCC+AGG 0.516160 1_4:-59109831 Msa0173750:intron
TCTGTATTTGGTGAACCAAA+AGG 0.519215 1_4:+59109846 None:intergenic
GAATTTGGAGCAGTAGGAGA+TGG 0.525396 1_4:-59111228 Msa0173750:intron
TGGATGTTAATGTTTGTACT+TGG 0.526630 1_4:+59109420 None:intergenic
GCCATCGGAAGTGAGATGTC+TGG 0.526771 1_4:-59109363 Msa0173750:CDS
TTAACGTCCGAGCAGTTCCA+TGG 0.530655 1_4:+59109000 None:intergenic
GTAGTCGAAACAGGGTTGCT+TGG 0.530724 1_4:-59108867 Msa0173750:CDS
TAATTTCCATCAAAAGTGGA+TGG 0.531088 1_4:-59109470 Msa0173750:CDS
AAACGCGCGGGCCTGATACA+AGG 0.531662 1_4:-59109090 Msa0173750:CDS
TTTGGTTCACCAAATACAGA+TGG 0.531824 1_4:-59109843 Msa0173750:CDS
ATAAAGACTTCTCCCGGGAG+GGG 0.536499 1_4:-59109279 Msa0173750:CDS
ATTAACATCCACAGGCTCAC+CGG 0.537205 1_4:-59109408 Msa0173750:CDS
GAAGGGAGTTGCCAGGTGGA+CGG 0.537707 1_4:-59110345 Msa0173750:CDS
AATAAAGACTTCTCCCGGGA+GGG 0.539545 1_4:-59109280 Msa0173750:CDS
ATACTCTGAATTGGTTTCCC+CGG 0.540672 1_4:-59108974 Msa0173750:CDS
TACCATCATATGGTAGGGGA+AGG 0.548235 1_4:-59110363 Msa0173750:CDS
GAACAAAACCTGCAATATAC+GGG 0.548268 1_4:+59110021 None:intergenic
CGACCCAATGTAGGCAACAA+TGG 0.548969 1_4:-59110195 Msa0173750:intron
TTGTCCTCCCGGTGAGCCTG+TGG 0.550617 1_4:+59109400 None:intergenic
TCCCTTCCCCTACCATATGA+TGG 0.550890 1_4:+59110361 None:intergenic
GTACAAACATTAACATCCAC+AGG 0.555385 1_4:-59109416 Msa0173750:CDS
ATACGAATTTAGGTAGAAGA+TGG 0.555815 1_4:+59111173 None:intergenic
CCCCTACCATATGATGGTAG+AGG 0.560136 1_4:+59110367 None:intergenic
AGGCTTCTATGCCGTCCACC+TGG 0.560231 1_4:+59110334 None:intergenic
TGCAGTAATACTTGGATCAA+CGG 0.561147 1_4:-59111052 Msa0173750:intron
TTAACATAACCACCCCTCCC+GGG 0.564851 1_4:+59109267 None:intergenic
ATGGTCAGGGGAGTATTTGG+TGG 0.566466 1_4:-59110164 Msa0173750:CDS
TTACTGTCGAGAATGTGATA+GGG 0.570895 1_4:-59109122 Msa0173750:CDS
TTGGTTCACCAAATACAGAT+GGG 0.571959 1_4:-59109842 Msa0173750:CDS
CAAAACTACCGGTTAACCAC+TGG 0.574055 1_4:+59111112 None:intergenic
TTAACATCCACAGGCTCACC+GGG 0.574227 1_4:-59109407 Msa0173750:CDS
AATTCGTATGCCGACAAAGG+TGG 0.577479 1_4:-59111159 Msa0173750:CDS
CCAAAATGGTGAATTCAAGA+AGG 0.579215 1_4:+59110053 None:intergenic
AATTTGGAGCAGTAGGAGAT+GGG 0.579376 1_4:-59111227 Msa0173750:intron
TCAGTTTAGTGGTAAGTGCA+GGG 0.580269 1_4:-59108663 Msa0173750:three_prime_UTR
GATGGAAATTAGATCATCGC+CGG 0.581092 1_4:+59109482 None:intergenic
TGTTGTCAAAGTGATATCAA+TGG 0.584592 1_4:-59108707 Msa0173750:three_prime_UTR
AAGACTTCTCCCGGGAGGGG+TGG 0.589573 1_4:-59109276 Msa0173750:CDS
TTATCAATTCGACTAAATGA+GGG 0.594556 1_4:+59110106 None:intergenic
GAAGACTGTTATATGAGCAC+CGG 0.595782 1_4:-59109501 Msa0173750:CDS
ATCCTCTACCATCATATGGT+AGG 0.598263 1_4:-59110369 Msa0173750:CDS
CGGTTAACCACTGGCCAGCA+GGG 0.600393 1_4:+59111121 None:intergenic
TTGGTGAACCAAAAGGAGCA+AGG 0.603870 1_4:+59109853 None:intergenic
AATGGAACTATAGATGGTCA+GGG 0.604106 1_4:-59110177 Msa0173750:CDS
TACTGTCGAGAATGTGATAG+GGG 0.606390 1_4:-59109121 Msa0173750:CDS
CTAAATTCGTATGCCGACAA+AGG 0.607464 1_4:-59111162 Msa0173750:CDS
CCATATCTCTAACATCACCT+TGG 0.608668 1_4:-59109238 Msa0173750:CDS
AATTTCCATCAAAAGTGGAT+GGG 0.610387 1_4:-59109469 Msa0173750:CDS
GGTAGGGGAAGGGAGTTGCC+AGG 0.612580 1_4:-59110352 Msa0173750:CDS
GCTGATGAGATCAAAACTAC+CGG 0.612616 1_4:+59111101 None:intergenic
GAGTTGCTCACAAGCTTCCG+GGG 0.613710 1_4:+59108957 None:intergenic
ACGAGTGCCGGAATCGCCAT+CGG 0.615761 1_4:-59109378 Msa0173750:CDS
GGGGAAAACATCAAACGCGC+GGG 0.616437 1_4:-59109102 Msa0173750:CDS
CCTCTACCATCATATGGTAG+GGG 0.616722 1_4:-59110367 Msa0173750:CDS
TCCAGACATCTCACTTCCGA+TGG 0.620855 1_4:+59109362 None:intergenic
ACCATCATATGGTAGGGGAA+GGG 0.626662 1_4:-59110362 Msa0173750:CDS
AGCAATATCTACATTAACCA+AGG 0.627118 1_4:+59109221 None:intergenic
TGTCCAACCACAAAGTGAGA+TGG 0.629482 1_4:+59111079 None:intergenic
CGAATTGATAAATTCAACAG+AGG 0.637343 1_4:-59110094 Msa0173750:CDS
AGGGGAAGGGAGTTGCCAGG+TGG 0.645313 1_4:-59110349 Msa0173750:CDS
CAGTTTAGTGGTAAGTGCAG+GGG 0.646160 1_4:-59108662 Msa0173750:three_prime_UTR
TAAGAATAAAGACTTCTCCC+GGG 0.654747 1_4:-59109284 Msa0173750:CDS
AGGGGAAAACATCAAACGCG+CGG 0.655278 1_4:-59109103 Msa0173750:CDS
GAATAAAGACTTCTCCCGGG+AGG 0.661627 1_4:-59109281 Msa0173750:CDS
GTGCAGGGGACTAATAACCA+TGG 0.661870 1_4:-59108648 Msa0173750:three_prime_UTR
ATCGGAAGTGAGATGTCTGG+AGG 0.664879 1_4:-59109360 Msa0173750:CDS
TGGGATCACTCTCAATACAA+AGG 0.671060 1_4:-59111208 Msa0173750:CDS
GAGTTCGAGTAGTCGAAACA+GGG 0.687667 1_4:-59108875 Msa0173750:CDS
TGACATTCTGAATTGTAACA+TGG 0.692990 1_4:+59109880 None:intergenic
TCAGTAATACTGACACTGTG+TGG 0.693416 1_4:+59111249 None:intergenic
ATGGAACTATAGATGGTCAG+GGG 0.713739 1_4:-59110176 Msa0173750:CDS
ACATCCACAGGCTCACCGGG+AGG 0.716687 1_4:-59109404 Msa0173750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAATATTTTTTGTGATTA+TGG + chr1_4:59109323-59109342 None:intergenic 10.0%
!! GTTTAGTATTTGTATAAATA+AGG - chr1_4:59109271-59109290 Msa0173750:CDS 15.0%
!! ACATTAATTAATGTATGCAT+AGG + chr1_4:59109019-59109038 None:intergenic 20.0%
!! TATGTTTGTATTTCATTAAC+CGG - chr1_4:59109459-59109478 Msa0173750:CDS 20.0%
!! ATATAAATATTGATGCCATT+TGG - chr1_4:59109737-59109756 Msa0173750:intron 20.0%
! AAATCACAGTTAATTCACAT+TGG - chr1_4:59109132-59109151 Msa0173750:CDS 25.0%
! AATCACAGTTAATTCACATT+GGG - chr1_4:59109133-59109152 Msa0173750:CDS 25.0%
! CTTCTAATCATAACTGAATA+AGG + chr1_4:59109252-59109271 None:intergenic 25.0%
! TTTAGAAAACTAGATAAGGA+AGG + chr1_4:59109359-59109378 None:intergenic 25.0%
!!! AGGTTTTAGAAAACTAGATA+AGG + chr1_4:59109363-59109382 None:intergenic 25.0%
! TACAAAATAATCCTTGCATA+GGG + chr1_4:59109396-59109415 None:intergenic 25.0%
! CATAACTTATATAGTAGTGA+TGG + chr1_4:59109443-59109462 None:intergenic 25.0%
! AATTAAACTACCAAGTTTGT+TGG + chr1_4:59109492-59109511 None:intergenic 25.0%
! TTATCAATTCGACTAAATGA+GGG + chr1_4:59109852-59109871 None:intergenic 25.0%
! TTTATCAATTCGACTAAATG+AGG + chr1_4:59109853-59109872 None:intergenic 25.0%
! ACTATTTCACAGTCTTAAAA+TGG + chr1_4:59110008-59110027 None:intergenic 25.0%
! ATATTGAATTCCTGTTCATT+TGG - chr1_4:59110221-59110240 Msa0173750:intron 25.0%
!!! GAGATATATTGATTTCAACA+AGG + chr1_4:59110308-59110327 None:intergenic 25.0%
! GATGAATATGGAATTTCATT+TGG - chr1_4:59110508-59110527 Msa0173750:intron 25.0%
!!! ATTAGATTCACTGGTTTATA+TGG - chr1_4:59110757-59110776 Msa0173750:intron 25.0%
! TAACAGTTTGAAAAATGATC+AGG + chr1_4:59111039-59111058 None:intergenic 25.0%
! TTACAAGTTAACAGATATCA+TGG - chr1_4:59111056-59111075 Msa0173750:CDS 25.0%
!! TTCAAGTATCTTTGAACTTT+TGG + chr1_4:59111158-59111177 None:intergenic 25.0%
! ACTGACTATTGTTATGATTA+AGG - chr1_4:59111343-59111362 Msa0173750:CDS 25.0%
CGAACTTCCTTAATGTTTAT+CGG + chr1_4:59108685-59108704 None:intergenic 30.0%
AGTGTCAGTATTACTGAATT+TGG - chr1_4:59108712-59108731 Msa0173750:three_prime_UTR 30.0%
ATACGAATTTAGGTAGAAGA+TGG + chr1_4:59108785-59108804 None:intergenic 30.0%
TTGCATAGGGTTAAAGTTAT+AGG + chr1_4:59109383-59109402 None:intergenic 30.0%
TATAACTTTAACCCTATGCA+AGG - chr1_4:59109382-59109401 Msa0173750:CDS 30.0%
GTACAAAATAATCCTTGCAT+AGG + chr1_4:59109397-59109416 None:intergenic 30.0%
CATTACACACTATATCGTAA+TGG + chr1_4:59109535-59109554 None:intergenic 30.0%
TTAAATTGTGCCCATAAATG+AGG + chr1_4:59109644-59109663 None:intergenic 30.0%
TTAACTGATGTCATCATAAC+AGG - chr1_4:59109660-59109679 Msa0173750:intron 30.0%
CGAATTGATAAATTCAACAG+AGG - chr1_4:59109861-59109880 Msa0173750:CDS 30.0%
AGAACAAAACCTGCAATATA+CGG + chr1_4:59109938-59109957 None:intergenic 30.0%
GTGAAATAGTAAAGCTTTCA+TGG - chr1_4:59110018-59110037 Msa0173750:intron 30.0%
TAGTAAAGCTTTCATGGTTA+TGG - chr1_4:59110024-59110043 Msa0173750:intron 30.0%
AGTAAAGCTTTCATGGTTAT+GGG - chr1_4:59110025-59110044 Msa0173750:intron 30.0%
TGACATTCTGAATTGTAACA+TGG + chr1_4:59110078-59110097 None:intergenic 30.0%
ATTAACAACTACATAGGGTA+TGG + chr1_4:59110176-59110195 None:intergenic 30.0%
TGTGCATTAACAACTACATA+GGG + chr1_4:59110181-59110200 None:intergenic 30.0%
TTGTGCATTAACAACTACAT+AGG + chr1_4:59110182-59110201 None:intergenic 30.0%
! TACTTCCTTTATGCCTTTTT+GGG + chr1_4:59110248-59110267 None:intergenic 30.0%
! ATACTTCCTTTATGCCTTTT+TGG + chr1_4:59110249-59110268 None:intergenic 30.0%
!!! CGAGTCTTTTGAAACAAAAT+TGG + chr1_4:59110375-59110394 None:intergenic 30.0%
!! TTTTGTTTCAAAAGACTCGT+CGG - chr1_4:59110376-59110395 Msa0173750:CDS 30.0%
AGAATCTGGACACAAATAAA+AGG + chr1_4:59110416-59110435 None:intergenic 30.0%
CATACATTATCAGAAGAATC+TGG + chr1_4:59110430-59110449 None:intergenic 30.0%
GATCTAATTTCCATCAAAAG+TGG - chr1_4:59110481-59110500 Msa0173750:intron 30.0%
TAATTTCCATCAAAAGTGGA+TGG - chr1_4:59110485-59110504 Msa0173750:intron 30.0%
AATTTCCATCAAAAGTGGAT+GGG - chr1_4:59110486-59110505 Msa0173750:intron 30.0%
TGGATGTTAATGTTTGTACT+TGG + chr1_4:59110538-59110557 None:intergenic 30.0%
!! TTGCACTTTTTGAATCAAAC+AGG + chr1_4:59110651-59110670 None:intergenic 30.0%
ATAAGAATAAAGACTTCTCC+CGG - chr1_4:59110670-59110689 Msa0173750:intron 30.0%
AGCAATATCTACATTAACCA+AGG + chr1_4:59110737-59110756 None:intergenic 30.0%
! GATATTGCTATTAGATTCAC+TGG - chr1_4:59110748-59110767 Msa0173750:intron 30.0%
ATACTCTACCTGTGATAAAA+AGG - chr1_4:59110809-59110828 Msa0173750:intron 30.0%
! ACAGTAATCCTTTTTATCAC+AGG + chr1_4:59110820-59110839 None:intergenic 30.0%
ATGTGAGCAAAAAAAATCCA+TGG - chr1_4:59110938-59110957 Msa0173750:intron 30.0%
TGTTGTCAAAGTGATATCAA+TGG - chr1_4:59111248-59111267 Msa0173750:intron 30.0%
! ATTGTAAAGTGTCAGTTTAG+TGG - chr1_4:59111281-59111300 Msa0173750:CDS 30.0%
!! CAGTGATAGTTTATTTTCCA+TGG + chr1_4:59111327-59111346 None:intergenic 30.0%
CTGCAAACCGATAAACATTA+AGG - chr1_4:59108675-59108694 Msa0173750:three_prime_UTR 35.0%
! ATTACTGAATTTGGAGCAGT+AGG - chr1_4:59108721-59108740 Msa0173750:three_prime_UTR 35.0%
GACAAAGATGCAGTAATACT+TGG - chr1_4:59108895-59108914 Msa0173750:CDS 35.0%
TGCAGTAATACTTGGATCAA+CGG - chr1_4:59108903-59108922 Msa0173750:CDS 35.0%
ACTGCATTGAACACAAACAA+AGG + chr1_4:59108946-59108965 None:intergenic 35.0%
! TTGTGTTCAATGCAGTTTTG+TGG - chr1_4:59108950-59108969 Msa0173750:CDS 35.0%
! ATGCATAGGAAGCATATCAT+AGG + chr1_4:59109005-59109024 None:intergenic 35.0%
! TGGCATTTGACTGTTTTCTA+GGG - chr1_4:59109193-59109212 Msa0173750:CDS 35.0%
!!! ATTTGACTGTTTTCTAGGGT+TGG - chr1_4:59109197-59109216 Msa0173750:CDS 35.0%
GTAATAAATCATGACAGCCA+AGG + chr1_4:59109230-59109249 None:intergenic 35.0%
! ATATCGTAATGGAGAACACA+TGG + chr1_4:59109524-59109543 None:intergenic 35.0%
! TGTTACAGAACTCTGAAGAT+TGG - chr1_4:59109553-59109572 Msa0173750:intron 35.0%
AATGGAACTATAGATGGTCA+GGG - chr1_4:59109778-59109797 Msa0173750:intron 35.0%
CCAAAATGGTGAATTCAAGA+AGG + chr1_4:59109905-59109924 None:intergenic 35.0%
! CCTTCTTGAATTCACCATTT+TGG - chr1_4:59109902-59109921 Msa0173750:intron 35.0%
GAACAAAACCTGCAATATAC+GGG + chr1_4:59109937-59109956 None:intergenic 35.0%
! TCTGTATTTGGTGAACCAAA+AGG + chr1_4:59110112-59110131 None:intergenic 35.0%
! TTTGGTTCACCAAATACAGA+TGG - chr1_4:59110112-59110131 Msa0173750:CDS 35.0%
TTGGTTCACCAAATACAGAT+GGG - chr1_4:59110113-59110132 Msa0173750:CDS 35.0%
CCTAAGGAAGAAAATTAACC+TGG + chr1_4:59110145-59110164 None:intergenic 35.0%
! CCAGGTTAATTTTCTTCCTT+AGG - chr1_4:59110142-59110161 Msa0173750:CDS 35.0%
! GTTAATTTTCTTCCTTAGGC+TGG - chr1_4:59110146-59110165 Msa0173750:CDS 35.0%
AAAAGTGGATGGGATGAATA+TGG - chr1_4:59110496-59110515 Msa0173750:intron 35.0%
GTACAAACATTAACATCCAC+AGG - chr1_4:59110539-59110558 Msa0173750:intron 35.0%
TAAGAATAAAGACTTCTCCC+GGG - chr1_4:59110671-59110690 Msa0173750:intron 35.0%
! TTATCACAGGTAGAGTATCT+GGG + chr1_4:59110807-59110826 None:intergenic 35.0%
! TTTATCACAGGTAGAGTATC+TGG + chr1_4:59110808-59110827 None:intergenic 35.0%
ATTACTGTCGAGAATGTGAT+AGG - chr1_4:59110832-59110851 Msa0173750:intron 35.0%
TTACTGTCGAGAATGTGATA+GGG - chr1_4:59110833-59110852 Msa0173750:intron 35.0%
CACCTTTAATACCTTGTATC+AGG + chr1_4:59110879-59110898 None:intergenic 35.0%
AAAAAATCCATGGAACTGCT+CGG - chr1_4:59110948-59110967 Msa0173750:intron 35.0%
CGTTAAAGGATACTCTGAAT+TGG - chr1_4:59110972-59110991 Msa0173750:intron 35.0%
ACGTGACCTAAAAAGTTGAA+TGG - chr1_4:59111211-59111230 Msa0173750:CDS 35.0%
! GCAACACCATTCAACTTTTT+AGG + chr1_4:59111220-59111239 None:intergenic 35.0%
!! CATTGATTTGTTGCAGCTCA+TGG - chr1_4:59108632-59108651 Msa0173750:three_prime_UTR 40.0%
TCAGTAATACTGACACTGTG+TGG + chr1_4:59108709-59108728 None:intergenic 40.0%
! AATTTGGAGCAGTAGGAGAT+GGG - chr1_4:59108728-59108747 Msa0173750:three_prime_UTR 40.0%
TGGGATCACTCTCAATACAA+AGG - chr1_4:59108747-59108766 Msa0173750:three_prime_UTR 40.0%
CTTTGTCGGCATACGAATTT+AGG + chr1_4:59108795-59108814 None:intergenic 40.0%
CTAAATTCGTATGCCGACAA+AGG - chr1_4:59108793-59108812 Msa0173750:three_prime_UTR 40.0%
GCTGATGAGATCAAAACTAC+CGG + chr1_4:59108857-59108876 None:intergenic 40.0%
!! GTTCAATGCAGTTTTGTGGA+AGG - chr1_4:59108954-59108973 Msa0173750:CDS 40.0%
GCATATCATAGGAACAGTAC+AGG + chr1_4:59108994-59109013 None:intergenic 40.0%
GTTAATTCACATTGGGAAGC+TGG - chr1_4:59109140-59109159 Msa0173750:CDS 40.0%
!! AAATGCCACGTTTGGTTTGA+CGG + chr1_4:59109181-59109200 None:intergenic 40.0%
AAACAGTCAAATGCCACGTT+TGG + chr1_4:59109189-59109208 None:intergenic 40.0%
! GTGGCATTTGACTGTTTTCT+AGG - chr1_4:59109192-59109211 Msa0173750:CDS 40.0%
!! CTAGGGTTGGTATATTACCT+TGG - chr1_4:59109210-59109229 Msa0173750:CDS 40.0%
ATAAGGAAGGAGACAAGAGA+GGG + chr1_4:59109346-59109365 None:intergenic 40.0%
TAGTAGTGATGGCATACGAA+GGG + chr1_4:59109432-59109451 None:intergenic 40.0%
ATAGTAGTGATGGCATACGA+AGG + chr1_4:59109433-59109452 None:intergenic 40.0%
ATCCTCTACCATCATATGGT+AGG - chr1_4:59109586-59109605 Msa0173750:intron 40.0%
TCCTCTACCATCATATGGTA+GGG - chr1_4:59109587-59109606 Msa0173750:intron 40.0%
GGCATAGAAGCCTCATTTAT+GGG - chr1_4:59109631-59109650 Msa0173750:intron 40.0%
GTTCCATTGTTGCCTACATT+GGG + chr1_4:59109766-59109785 None:intergenic 40.0%
AGTTCCATTGTTGCCTACAT+TGG + chr1_4:59109767-59109786 None:intergenic 40.0%
GGCAACAATGGAACTATAGA+TGG - chr1_4:59109772-59109791 Msa0173750:intron 40.0%
CAATGGAACTATAGATGGTC+AGG - chr1_4:59109777-59109796 Msa0173750:intron 40.0%
ATGGAACTATAGATGGTCAG+GGG - chr1_4:59109779-59109798 Msa0173750:intron 40.0%
ACGGGATGAATAGTCCAAAA+TGG + chr1_4:59109919-59109938 None:intergenic 40.0%
CTATTCATCCCGTATATTGC+AGG - chr1_4:59109926-59109945 Msa0173750:intron 40.0%
!!! CAGGTTTTGTTCTCTTCAGA+AGG - chr1_4:59109945-59109964 Msa0173750:intron 40.0%
GGATCAATCCCATCTGTATT+TGG + chr1_4:59110124-59110143 None:intergenic 40.0%
AATACAGATGGGATTGATCC+AGG - chr1_4:59110124-59110143 Msa0173750:CDS 40.0%
!!! CTTTTTGGGTCCAAATGAAC+AGG + chr1_4:59110234-59110253 None:intergenic 40.0%
CTGTTCATTTGGACCCAAAA+AGG - chr1_4:59110232-59110251 Msa0173750:intron 40.0%
! TTGGACCCAAAAAGGCATAA+AGG - chr1_4:59110240-59110259 Msa0173750:intron 40.0%
TGTCATCTGTCAACTATGCT+AGG + chr1_4:59110330-59110349 None:intergenic 40.0%
GAAGACTGTTATATGAGCAC+CGG - chr1_4:59110454-59110473 Msa0173750:intron 40.0%
GATGGAAATTAGATCATCGC+CGG + chr1_4:59110476-59110495 None:intergenic 40.0%
!! TTCATCCCATCCACTTTTGA+TGG + chr1_4:59110494-59110513 None:intergenic 40.0%
CCAAGGTGATGTTAGAGATA+TGG + chr1_4:59110720-59110739 None:intergenic 40.0%
CCATATCTCTAACATCACCT+TGG - chr1_4:59110717-59110736 Msa0173750:intron 40.0%
TACTGTCGAGAATGTGATAG+GGG - chr1_4:59110834-59110853 Msa0173750:intron 40.0%
! GGCCTGATACAAGGTATTAA+AGG - chr1_4:59110874-59110893 Msa0173750:intron 40.0%
! ATACTCTGAATTGGTTTCCC+CGG - chr1_4:59110981-59111000 Msa0173750:intron 40.0%
TCAGTTTAGTGGTAAGTGCA+GGG - chr1_4:59111292-59111311 Msa0173750:CDS 40.0%
! GAATTTGGAGCAGTAGGAGA+TGG - chr1_4:59108727-59108746 Msa0173750:three_prime_UTR 45.0%
AATTCGTATGCCGACAAAGG+TGG - chr1_4:59108796-59108815 Msa0173750:three_prime_UTR 45.0%
! CAAAACTACCGGTTAACCAC+TGG + chr1_4:59108846-59108865 None:intergenic 45.0%
TCATCAGCCATCTCACTTTG+TGG - chr1_4:59108869-59108888 Msa0173750:CDS 45.0%
TGTCCAACCACAAAGTGAGA+TGG + chr1_4:59108879-59108898 None:intergenic 45.0%
CGAAGCGTGAAAACAAACAC+TGG + chr1_4:59109109-59109128 None:intergenic 45.0%
GATAAGGAAGGAGACAAGAG+AGG + chr1_4:59109347-59109366 None:intergenic 45.0%
!! CAAGTTTGTTGGACTCAAGC+CGG + chr1_4:59109481-59109500 None:intergenic 45.0%
GTGGATCCTCTACCATCATA+TGG - chr1_4:59109582-59109601 Msa0173750:intron 45.0%
CCTCTACCATCATATGGTAG+GGG - chr1_4:59109588-59109607 Msa0173750:intron 45.0%
TACCATCATATGGTAGGGGA+AGG - chr1_4:59109592-59109611 Msa0173750:intron 45.0%
ACCATCATATGGTAGGGGAA+GGG - chr1_4:59109593-59109612 Msa0173750:intron 45.0%
CGGCATAGAAGCCTCATTTA+TGG - chr1_4:59109630-59109649 Msa0173750:intron 45.0%
!! TAGATGGTCAGGGGAGTATT+TGG - chr1_4:59109788-59109807 Msa0173750:intron 45.0%
! GTCACAATCCTTGCTCCTTT+TGG - chr1_4:59110094-59110113 Msa0173750:CDS 45.0%
TTGGTGAACCAAAAGGAGCA+AGG + chr1_4:59110105-59110124 None:intergenic 45.0%
ATTAACATCCACAGGCTCAC+CGG - chr1_4:59110547-59110566 Msa0173750:intron 45.0%
AATAAAGACTTCTCCCGGGA+GGG - chr1_4:59110675-59110694 Msa0173750:intron 45.0%
TTTAACATAACCACCCCTCC+CGG + chr1_4:59110692-59110711 None:intergenic 45.0%
TCTGGGTCATATGAGTCATC+TGG + chr1_4:59110790-59110809 None:intergenic 45.0%
! TTGAGTTGCTCACAAGCTTC+CGG + chr1_4:59111003-59111022 None:intergenic 45.0%
AGAGTTCGAGTAGTCGAAAC+AGG - chr1_4:59111079-59111098 Msa0173750:CDS 45.0%
GAGTTCGAGTAGTCGAAACA+GGG - chr1_4:59111080-59111099 Msa0173750:CDS 45.0%
GTCAGTTTAGTGGTAAGTGC+AGG - chr1_4:59111291-59111310 Msa0173750:CDS 45.0%
CAGTTTAGTGGTAAGTGCAG+GGG - chr1_4:59111293-59111312 Msa0173750:CDS 45.0%
GTTGCAGCTCATGGACAGTT+TGG - chr1_4:59108641-59108660 Msa0173750:three_prime_UTR 50.0%
AAGCTTGGCTCCACCTTTGT+CGG + chr1_4:59108809-59108828 None:intergenic 50.0%
CAGCCATCTCACTTTGTGGT+TGG - chr1_4:59108873-59108892 Msa0173750:CDS 50.0%
CGGCTTGAGTCCAACAAACT+TGG - chr1_4:59109479-59109498 Msa0173750:CDS 50.0%
! CTCTGAAGATTGGCCAGTTG+TGG - chr1_4:59109563-59109582 Msa0173750:intron 50.0%
GATGGTAGAGGATCCACAAC+TGG + chr1_4:59109579-59109598 None:intergenic 50.0%
CCCCTACCATATGATGGTAG+AGG + chr1_4:59109591-59109610 None:intergenic 50.0%
TCCCTTCCCCTACCATATGA+TGG + chr1_4:59109597-59109616 None:intergenic 50.0%
CGACCCAATGTAGGCAACAA+TGG - chr1_4:59109760-59109779 Msa0173750:intron 50.0%
!! ATGGTCAGGGGAGTATTTGG+TGG - chr1_4:59109791-59109810 Msa0173750:intron 50.0%
TTAACATCCACAGGCTCACC+GGG - chr1_4:59110548-59110567 Msa0173750:intron 50.0%
TCCAGACATCTCACTTCCGA+TGG + chr1_4:59110596-59110615 None:intergenic 50.0%
ATCGGAAGTGAGATGTCTGG+AGG - chr1_4:59110595-59110614 Msa0173750:intron 50.0%
GAATAAAGACTTCTCCCGGG+AGG - chr1_4:59110674-59110693 Msa0173750:intron 50.0%
ATAAAGACTTCTCCCGGGAG+GGG - chr1_4:59110676-59110695 Msa0173750:intron 50.0%
TTAACATAACCACCCCTCCC+GGG + chr1_4:59110691-59110710 None:intergenic 50.0%
AGGGGAAAACATCAAACGCG+CGG - chr1_4:59110852-59110871 Msa0173750:intron 50.0%
TTAACGTCCGAGCAGTTCCA+TGG + chr1_4:59110958-59110977 None:intergenic 50.0%
TGGAACTGCTCGGACGTTAA+AGG - chr1_4:59110958-59110977 Msa0173750:intron 50.0%
TGAGTTGCTCACAAGCTTCC+GGG + chr1_4:59111002-59111021 None:intergenic 50.0%
GTAGTCGAAACAGGGTTGCT+TGG - chr1_4:59111088-59111107 Msa0173750:CDS 50.0%
TGCTTGGTTGCAGTCTTGGT+AGG - chr1_4:59111104-59111123 Msa0173750:CDS 50.0%
GTGCAGGGGACTAATAACCA+TGG - chr1_4:59111307-59111326 Msa0173750:CDS 50.0%
! GCCAAGCTTTTTGTCCCTGC+TGG - chr1_4:59108820-59108839 Msa0173750:three_prime_UTR 55.0%
GCCAGCAGGGACAAAAAGCT+TGG + chr1_4:59108824-59108843 None:intergenic 55.0%
! TTTTTGTCCCTGCTGGCCAG+TGG - chr1_4:59108827-59108846 Msa0173750:three_prime_UTR 55.0%
!! TGCCACGTTTGGTTTGACGG+CGG + chr1_4:59109178-59109197 None:intergenic 55.0%
GCCATTTGGCGACCCAATGT+AGG - chr1_4:59109751-59109770 Msa0173750:intron 55.0%
GCCTACATTGGGTCGCCAAA+TGG + chr1_4:59109755-59109774 None:intergenic 55.0%
GGGTATGGCATTCCAGCCTA+AGG + chr1_4:59110161-59110180 None:intergenic 55.0%
CTCACTTCCGATGGCGATTC+CGG + chr1_4:59110587-59110606 None:intergenic 55.0%
GCCATCGGAAGTGAGATGTC+TGG - chr1_4:59110592-59110611 Msa0173750:intron 55.0%
GGGGAAAACATCAAACGCGC+GGG - chr1_4:59110853-59110872 Msa0173750:intron 55.0%
GAGTTGCTCACAAGCTTCCG+GGG + chr1_4:59111001-59111020 None:intergenic 55.0%
GGGTTGCTTGGTTGCAGTCT+TGG - chr1_4:59111100-59111119 Msa0173750:CDS 55.0%
CGGTTAACCACTGGCCAGCA+GGG + chr1_4:59108837-59108856 None:intergenic 60.0%
CCTGCTGGCCAGTGGTTAAC+CGG - chr1_4:59108835-59108854 Msa0173750:exon 60.0%
!! CACGTTTGGTTTGACGGCGG+GGG + chr1_4:59109175-59109194 None:intergenic 60.0%
!! CCACGTTTGGTTTGACGGCG+GGG + chr1_4:59109176-59109195 None:intergenic 60.0%
!! GCCACGTTTGGTTTGACGGC+GGG + chr1_4:59109177-59109196 None:intergenic 60.0%
GAAGGGAGTTGCCAGGTGGA+CGG - chr1_4:59109610-59109629 Msa0173750:intron 60.0%
AGGCTTCTATGCCGTCCACC+TGG + chr1_4:59109624-59109643 None:intergenic 60.0%
!! ACGAGTGCCGGAATCGCCAT+CGG - chr1_4:59110577-59110596 Msa0173750:intron 60.0%
AAACGCGCGGGCCTGATACA+AGG - chr1_4:59110865-59110884 Msa0173750:intron 60.0%
CCGGTTAACCACTGGCCAGC+AGG + chr1_4:59108838-59108857 None:intergenic 65.0%
CCCCGCCGTCAAACCAAACG+TGG - chr1_4:59109173-59109192 Msa0173750:CDS 65.0%
GGTAGGGGAAGGGAGTTGCC+AGG - chr1_4:59109603-59109622 Msa0173750:intron 65.0%
AGGGGAAGGGAGTTGCCAGG+TGG - chr1_4:59109606-59109625 Msa0173750:intron 65.0%
ACATCCACAGGCTCACCGGG+AGG - chr1_4:59110551-59110570 Msa0173750:intron 65.0%
TTGTCCTCCCGGTGAGCCTG+TGG + chr1_4:59110558-59110577 None:intergenic 65.0%
ACCGGGAGGACAACGAGTGC+CGG - chr1_4:59110565-59110584 Msa0173750:intron 65.0%
TCCGGCACTCGTTGTCCTCC+CGG + chr1_4:59110569-59110588 None:intergenic 65.0%
AAGACTTCTCCCGGGAGGGG+TGG - chr1_4:59110679-59110698 Msa0173750:intron 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 59108607 59111370 59108607 ID=Msa0173750;Name=Msa0173750
chr1_4 mRNA 59108607 59111370 59108607 ID=Msa0173750-mRNA-1;Parent=Msa0173750;Name=Msa0173750-mRNA-1;_AED=0.11;_eAED=0.11;_QI=0|0.8|0.66|1|0.8|0.66|6|245|452
chr1_4 exon 59108607 59109546 59108607 ID=Msa0173750-mRNA-1:exon:18531;Parent=Msa0173750-mRNA-1
chr1_4 exon 59109832 59109902 59109832 ID=Msa0173750-mRNA-1:exon:18530;Parent=Msa0173750-mRNA-1
chr1_4 exon 59110030 59110204 59110030 ID=Msa0173750-mRNA-1:exon:18529;Parent=Msa0173750-mRNA-1
chr1_4 exon 59110296 59110416 59110296 ID=Msa0173750-mRNA-1:exon:18528;Parent=Msa0173750-mRNA-1
chr1_4 exon 59111053 59111237 59111053 ID=Msa0173750-mRNA-1:exon:18527;Parent=Msa0173750-mRNA-1
chr1_4 exon 59111259 59111370 59111259 ID=Msa0173750-mRNA-1:exon:18526;Parent=Msa0173750-mRNA-1
chr1_4 CDS 59111259 59111370 59111259 ID=Msa0173750-mRNA-1:cds;Parent=Msa0173750-mRNA-1
chr1_4 CDS 59111053 59111237 59111053 ID=Msa0173750-mRNA-1:cds;Parent=Msa0173750-mRNA-1
chr1_4 CDS 59110296 59110416 59110296 ID=Msa0173750-mRNA-1:cds;Parent=Msa0173750-mRNA-1
chr1_4 CDS 59110030 59110204 59110030 ID=Msa0173750-mRNA-1:cds;Parent=Msa0173750-mRNA-1
chr1_4 CDS 59109832 59109902 59109832 ID=Msa0173750-mRNA-1:cds;Parent=Msa0173750-mRNA-1
chr1_4 CDS 59108852 59109546 59108852 ID=Msa0173750-mRNA-1:cds;Parent=Msa0173750-mRNA-1
chr1_4 three_prime_UTR 59108607 59108851 59108607 ID=Msa0173750-mRNA-1:three_prime_utr;Parent=Msa0173750-mRNA-1
Gene Sequence

>Msa0173750

CTAGTGGATGTGTTTTTGGTACTTGCATTGATTTGTTGCAGCTCATGGACAGTTTGGAGCAGCTCAAGCTGCAAACCGATAAACATTAAGGAAGTTCGACCACACAGTGTCATAGGAGATGGGATCACTCTCAATACAAAGGCGTTTCAGAATGCCATCTTCTACCTAAATTCGTATGCCGACAAAGGTGGAGCCAAGCTTTTTGTCCCTGCTGGCCAGTGGTTAACCGGTAGTTTTGATCTCATCAGCCATCTCACTTTGTGGTTGGACAAAGATGCAGTAATACTTGGATCAACGAACTCTGAAGATTGGCCAGTTGTGGATCCTCTACCATCATATGGTAGGGGAAGGGAGTTGCCAGGTGGACGGCATAGAAGCCTCATTTATGGGCACAATTTAACTGATGTCATCATAACAGGCAACAATGGAACTATAGATGGTCAGGGGAGTATTTGGTGGAGCAAATTTCGTAACAAAACACTCGATCACACACGCCCTCATTTAGTCGAATTGATAAATTCAACAGAGGTTCTCATTTCAAATGTAACCTTCTTGAATTCACCATTTTGGACTATTCATCCCGTATATTGCAGCCATGTTACAATTCAGAATGTCACAATCCTTGCTCCTTTTGGTTCACCAAATACAGATGGGATTGATCCAGATTCTTCTGATAATGTATGCATTGAAGACTGTTATATGAGCACCGGCGATGATCTAATTTCCATCAAAAGTGGATGGGATGAATATGGAATTTCATTTGGTCGACCAAGTACAAACATTAACATCCACAGGCTCACCGGGAGGACAACGAGTGCCGGAATCGCCATCGGAAGTGAGATGTCTGGAGGTGTTTCAGAAGTTCATGCAGAAGATATTTACCTGTTTGATTCAAAAAGTGCAATAAGAATAAAGACTTCTCCCGGGAGGGGTGGTTATGTTAAAAATGTCCATATCTCTAACATCACCTTGGTTAATGTAGATATTGCTATTAGATTCACTGGTTTATATGGAGAGCATCCAGATGACTCATATGACCCAGATACTCTACCTGTGATAAAAAGGATTACTGTCGAGAATGTGATAGGGGAAAACATCAAACGCGCGGGCCTGATACAAGGTATTAAAGGTGACAACTTTGTCGATATCTGTCTATCAAACATCACTCTTAATGTGAGCAAAAAAAATCCATGGAACTGCTCGGACGTTAAAGGATACTCTGAATTGGTTTCCCCGGAAGCTTGTGAGCAACTCAATGAGAGAATATTTCCTGATCATTTTTCAAACTGTTACAAGTTAACAGATATCATGGAGAGTTCGAGTAGTCGAAACAGGGTTGCTTGGTTGCAGTCTTGGTAG
Protein sequence

>Msa0173750

LVDVFLVLALICCSSWTVWSSSSCKPINIKEVRPHSVIGDGITLNTKAFQNAIFYLNSYADKGGAKLFVPAGQWLTGSFDLISHLTLWLDKDAVILGSTNSEDWPVVDPLPSYGRGRELPGGRHRSLIYGHNLTDVIITGNNGTIDGQGSIWWSKFRNKTLDHTRPHLVELINSTEVLISNVTFLNSPFWTIHPVYCSHVTIQNVTILAPFGSPNTDGIDPDSSDNVCIEDCYMSTGDDLISIKSGWDEYGISFGRPSTNINIHRLTGRTTSAGIAIGSEMSGGVSEVHAEDIYLFDSKSAIRIKTSPGRGGYVKNVHISNITLVNVDIAIRFTGLYGEHPDDSYDPDTLPVIKRITVENVIGENIKRAGLIQGIKGDNFVDICLSNITLNVSKKNPWNCSDVKGYSELVSPEACEQLNERIFPDHFSNCYKLTDIMESSSSRNRVAWLQSW*