AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0245000

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0245000 MtrunA17_Chr2g0298891 93.785 354 19 1 1 354 1 351 0.0 646
Msa0245000 MtrunA17_Chr4g0071581 67.135 356 94 5 1 352 2 338 1.96e-162 457
Msa0245000 MtrunA17_Chr4g0058541 47.303 241 126 1 47 287 208 447 7.78e-59 198
Msa0245000 MtrunA17_Chr4g0058541 30.061 163 111 2 43 203 273 434 8.87e-12 66.2
Msa0245000 MtrunA17_Chr3g0116991 45.528 246 130 2 42 287 203 444 3.04e-55 188
Msa0245000 MtrunA17_Chr5g0412701 43.952 248 138 1 40 287 148 394 3.47e-50 174
Msa0245000 MtrunA17_Chr5g0407461 40.167 239 141 2 47 285 305 541 8.97e-43 156
Msa0245000 MtrunA17_Chr5g0407461 40.351 171 99 2 99 269 264 431 3.01e-27 112
Msa0245000 MtrunA17_Chr8g0370111 39.773 264 152 3 47 305 294 555 1.84e-42 155
Msa0245000 MtrunA17_Chr4g0054721 38.346 266 139 3 44 286 219 482 9.79e-41 149
Msa0245000 MtrunA17_Chr4g0054721 36.782 174 107 2 99 272 204 374 2.07e-22 98.2
Msa0245000 MtrunA17_Chr8g0363851 35.294 187 117 4 75 260 592 775 1.07e-17 84.7
Msa0245000 MtrunA17_Chr8g0363851 33.173 208 134 5 80 286 644 847 1.83e-15 78.2
Msa0245000 MtrunA17_Chr8g0363851 32.353 204 122 7 47 237 703 903 2.92e-11 65.1
Msa0245000 MtrunA17_Chr8g0357151 35.632 261 155 12 19 269 245 502 1.07e-16 81.6
Msa0245000 MtrunA17_Chr8g0357151 36.574 216 125 11 63 269 194 406 5.46e-14 73.6
Msa0245000 MtrunA17_Chr8g0357151 33.597 253 157 11 19 263 317 566 2.74e-13 71.2
Msa0245000 MtrunA17_Chr8g0357151 34.529 223 134 11 56 269 115 334 2.68e-12 68.2
Msa0245000 MtrunA17_Chr8g0363891 36.916 214 119 9 54 264 162 362 4.18e-16 79.7
Msa0245000 MtrunA17_Chr8g0363891 34.409 186 117 5 77 260 129 311 1.30e-13 72.0
Msa0245000 MtrunA17_Chr8g0363891 33.333 189 120 6 77 263 82 266 9.13e-12 66.6
Msa0245000 MtrunA17_Chr8g0363891 36.652 221 114 12 51 263 90 292 5.23e-11 63.9
Msa0245000 MtrunA17_Chr3g0086371 30.804 224 124 6 46 266 24 219 1.30e-15 75.9
Msa0245000 MtrunA17_Chr3g0086371 27.586 203 121 3 72 274 27 203 3.24e-14 72.0
Msa0245000 MtrunA17_Chr1g0165141 29.740 269 170 13 56 310 237 500 1.73e-15 78.2
Msa0245000 MtrunA17_Chr1g0165141 29.018 224 145 8 49 267 94 308 1.50e-12 68.9
Msa0245000 MtrunA17_Chr1g0165141 29.960 247 162 10 57 296 190 432 1.60e-11 65.9
Msa0245000 MtrunA17_Chr8g0357171 36.620 213 122 12 70 273 166 374 2.08e-15 77.8
Msa0245000 MtrunA17_Chr8g0357171 32.227 211 103 8 82 263 116 315 1.92e-11 65.5
Msa0245000 MtrunA17_Chr8g0357171 31.304 230 141 11 57 272 201 427 5.32e-11 64.3
Msa0245000 MtrunA17_Chr2g0317831 31.280 211 140 5 63 272 67 273 8.67e-15 75.5
Msa0245000 MtrunA17_Chr2g0317831 27.347 245 149 5 47 288 364 582 1.72e-13 71.6
Msa0245000 MtrunA17_Chr4g0012841 35.071 211 126 11 70 272 150 357 2.71e-14 74.3
Msa0245000 MtrunA17_Chr8g0357191 36.792 212 124 10 63 267 254 462 5.89e-14 73.2
Msa0245000 MtrunA17_Chr8g0357191 32.927 246 131 12 56 269 127 370 1.58e-13 72.0
Msa0245000 MtrunA17_Chr7g0215641 36.022 186 107 8 90 267 90 271 1.26e-13 72.0
Msa0245000 MtrunA17_Chr1g0165221 31.963 219 141 8 56 268 213 429 2.77e-13 71.2
Msa0245000 MtrunA17_Chr1g0165221 31.905 210 135 8 64 267 101 308 9.16e-12 66.6
Msa0245000 MtrunA17_Chr2g0304481 34.000 200 125 7 55 249 411 608 4.37e-13 70.9
Msa0245000 MtrunA17_Chr7g0232211 31.707 287 135 13 67 295 76 359 5.43e-13 70.5
Msa0245000 MtrunA17_Chr8g0362431 30.542 203 129 7 70 265 151 348 7.14e-13 69.7
Msa0245000 MtrunA17_Chr4g0073641 31.925 213 132 8 67 267 350 561 9.31e-13 69.7
Msa0245000 MtrunA17_Chr2g0296601 33.333 207 127 11 69 267 601 804 1.28e-12 69.3
Msa0245000 MtrunA17_Chr2g0296591 33.333 207 127 11 69 267 601 804 1.50e-12 68.9
Msa0245000 MtrunA17_Chr1g0203251 26.159 302 184 8 19 284 405 703 2.94e-12 68.2
Msa0245000 MtrunA17_Chr3g0105881 29.064 203 134 5 73 269 136 334 5.29e-12 66.6
Msa0245000 MtrunA17_Chr5g0446871 37.736 159 91 8 63 215 281 437 5.86e-12 67.0
Msa0245000 MtrunA17_Chr7g0217081 35.514 214 124 11 63 267 133 341 7.11e-12 67.0
Msa0245000 MtrunA17_Chr1g0165901 32.692 208 130 9 70 269 290 495 8.41e-12 66.6
Msa0245000 MtrunA17_Chr8g0354031 39.355 155 87 7 67 215 206 359 9.86e-12 66.2
Msa0245000 MtrunA17_Chr8g0362931 32.353 238 143 11 46 267 186 421 1.00e-11 66.6
Msa0245000 MtrunA17_Chr8g0362931 35.526 152 92 6 69 215 631 781 7.26e-11 63.9
Msa0245000 MtrunA17_Chr8g0363111 36.076 158 95 6 63 215 440 596 1.10e-11 66.2
Msa0245000 MtrunA17_Chr8g0363111 30.000 220 133 9 70 272 175 390 7.41e-11 63.9
Msa0245000 MtrunA17_Chr1g0165211 35.052 194 117 9 67 254 603 793 1.31e-11 66.2
Msa0245000 MtrunA17_Chr1g0165211 30.088 226 148 10 50 268 322 544 2.43e-11 65.5
Msa0245000 MtrunA17_Chr7g0232451 34.300 207 128 8 67 267 120 324 1.86e-11 65.5
Msa0245000 MtrunA17_Chr3g0128731 30.769 221 139 9 56 265 269 486 1.98e-11 65.5
Msa0245000 MtrunA17_Chr5g0439291 33.014 209 113 8 63 268 132 316 2.15e-11 65.5
Msa0245000 MtrunA17_Chr7g0244931 31.963 219 135 8 57 265 337 551 2.47e-11 65.1
Msa0245000 MtrunA17_Chr3g0089251 41.176 102 58 2 160 260 606 706 2.68e-11 65.1
Msa0245000 MtrunA17_Chr3g0089961 45.349 86 46 1 174 259 595 679 2.70e-11 65.1
Msa0245000 MtrunA17_Chr1g0165191 34.536 194 118 9 67 254 583 773 3.31e-11 65.1
Msa0245000 MtrunA17_Chr7g0215681 35.870 184 110 8 90 267 110 291 3.76e-11 64.7
Msa0245000 MtrunA17_Chr7g0232131 30.450 289 139 14 67 296 85 370 3.93e-11 64.7
Msa0245000 MtrunA17_Chr1g0204461 29.767 215 119 9 57 265 316 504 4.64e-11 64.3
Msa0245000 MtrunA17_Chr1g0204461 32.864 213 134 9 67 273 87 296 9.72e-11 63.5
Msa0245000 MtrunA17_Chr5g0436901 35.567 194 114 11 80 265 67 257 4.72e-11 63.5
Msa0245000 MtrunA17_Chr1g0165951 32.759 232 145 11 43 266 98 326 4.81e-11 64.3
Msa0245000 MtrunA17_Chr3g0089501 45.977 87 46 1 174 260 590 675 5.32e-11 64.3
Msa0245000 MtrunA17_Chr3g0089601 45.977 87 46 1 174 260 590 675 5.45e-11 64.3
Msa0245000 MtrunA17_Chr7g0241921 31.336 217 140 9 56 265 371 585 5.90e-11 63.9
Msa0245000 MtrunA17_Chr6g0487031 28.340 247 126 9 56 274 64 287 6.70e-11 63.9
Msa0245000 MtrunA17_Chr1g0176301 34.756 164 100 7 67 225 200 361 7.05e-11 63.5
Msa0245000 MtrunA17_Chr7g0215671 28.235 255 146 8 18 267 40 262 7.36e-11 63.5
Msa0245000 MtrunA17_Chr4g0035444 29.353 201 134 8 69 264 411 608 7.71e-11 63.5
Msa0245000 MtrunA17_Chr2g0319851 26.384 307 184 13 50 322 623 921 7.95e-11 63.5
Msa0245000 MtrunA17_Chr2g0315071 31.390 223 136 12 56 267 12 228 8.76e-11 63.2
Msa0245000 MtrunA17_Chr2g0314001 30.288 208 129 9 73 267 277 481 9.06e-11 63.5
Msa0245000 MtrunA17_Chr2g0312651 31.646 237 136 11 67 293 509 729 9.98e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0245000 AT2G19330.1 64.465 318 108 2 40 352 58 375 1.47e-140 404
Msa0245000 AT4G29880.1 62.264 318 115 2 40 352 51 368 1.16e-126 369
Msa0245000 AT4G26050.1 60.976 328 118 5 34 351 46 373 5.19e-125 365
Msa0245000 AT3G11330.1 49.180 244 123 1 44 287 200 442 3.29e-58 196
Msa0245000 AT3G11330.1 32.298 161 106 2 43 201 268 427 8.35e-12 66.6
Msa0245000 AT5G05850.1 48.400 250 128 1 41 290 203 451 3.92e-57 194
Msa0245000 AT3G26500.1 40.856 257 139 2 44 300 162 405 1.33e-51 178
Msa0245000 AT1G12970.1 40.467 257 140 2 44 300 163 406 3.88e-49 171
Msa0245000 AT4G35470.1 38.168 262 157 3 47 305 274 533 1.21e-42 155
Msa0245000 AT4G35470.1 39.196 199 107 3 98 285 232 427 1.60e-22 99.0
Msa0245000 AT2G17440.1 39.924 263 152 3 47 305 258 518 6.35e-40 148
Msa0245000 AT4G35470.2 40.171 234 138 2 47 280 274 505 1.25e-39 147
Msa0245000 AT4G35470.2 39.196 199 107 3 98 285 232 427 2.06e-22 99.0
Msa0245000 AT4G35470.2 32.571 175 114 3 43 215 316 488 1.48e-15 78.2
Msa0245000 AT3G15410.1 35.071 211 128 5 63 270 66 270 2.50e-18 86.7
Msa0245000 AT3G15410.1 35.122 205 128 4 69 272 49 249 8.73e-18 85.1
Msa0245000 AT3G15410.1 28.723 282 166 6 46 298 72 347 5.03e-16 79.7
Msa0245000 AT3G15410.1 24.113 282 175 6 20 288 326 581 3.55e-14 73.9
Msa0245000 AT3G15410.2 34.450 209 132 4 63 270 72 276 2.84e-18 86.7
Msa0245000 AT3G15410.2 35.122 205 128 4 69 272 55 255 9.65e-18 85.1
Msa0245000 AT3G15410.2 28.723 282 166 6 46 298 78 353 5.45e-16 79.7
Msa0245000 AT3G15410.2 24.113 282 175 6 20 288 332 587 3.18e-14 74.3
Msa0245000 AT2G30105.1 34.211 190 109 5 101 278 141 326 2.65e-16 79.7
Msa0245000 AT2G30105.1 41.964 112 63 2 70 181 207 316 7.77e-15 75.5
Msa0245000 AT4G28490.1 33.945 218 127 12 45 247 87 302 1.92e-14 75.1
Msa0245000 AT2G34930.1 33.766 231 136 10 47 263 204 431 1.98e-14 75.1
Msa0245000 AT1G27170.1 30.698 215 121 5 69 258 769 980 3.48e-14 74.7
Msa0245000 AT1G27170.2 30.698 215 121 5 69 258 769 980 4.41e-14 74.3
Msa0245000 AT5G07910.1 27.586 232 137 6 46 274 25 228 1.82e-13 70.1
Msa0245000 AT2G15080.1 33.632 223 132 12 53 267 104 318 2.01e-13 72.0
Msa0245000 AT2G15080.2 33.632 223 132 12 53 267 104 318 2.01e-13 72.0
Msa0245000 AT2G33170.2 32.710 214 135 9 66 272 86 297 2.19e-13 72.0
Msa0245000 AT2G33170.1 32.710 214 135 9 66 272 86 297 2.19e-13 72.0
Msa0245000 AT3G56370.1 33.721 258 134 11 42 265 69 323 2.29e-13 72.0
Msa0245000 AT3G56370.1 32.365 241 146 9 32 263 226 458 6.69e-13 70.5
Msa0245000 AT1G69550.1 39.171 217 121 11 53 262 920 1132 2.56e-13 72.0
Msa0245000 AT1G69550.1 38.660 194 110 9 75 262 871 1061 3.60e-13 71.6
Msa0245000 AT1G69550.1 38.739 222 124 11 47 260 985 1202 1.88e-12 69.3
Msa0245000 AT1G69550.1 31.600 250 138 9 44 262 742 989 6.65e-11 64.3
Msa0245000 AT5G44700.1 33.766 231 141 11 44 265 266 493 4.74e-13 71.2
Msa0245000 AT4G04220.1 32.937 252 145 12 28 263 67 310 1.56e-12 69.3
Msa0245000 AT3G11080.2 34.783 184 92 7 87 266 123 282 1.57e-12 69.3
Msa0245000 AT3G11080.1 34.783 184 92 7 87 266 123 282 1.57e-12 69.3
Msa0245000 AT1G27180.1 29.577 213 144 6 49 258 945 1154 2.84e-12 68.6
Msa0245000 AT1G27180.2 29.577 213 144 6 49 258 783 992 3.05e-12 68.6
Msa0245000 AT5G07180.1 33.803 213 132 9 67 273 75 284 3.14e-12 68.6
Msa0245000 AT1G27180.3 29.577 213 144 6 49 258 783 992 3.45e-12 68.2
Msa0245000 AT1G27180.3 30.631 222 144 7 49 265 830 1046 3.26e-11 65.5
Msa0245000 AT5G46330.2 33.891 239 118 12 67 268 164 399 3.95e-12 68.2
Msa0245000 AT5G46330.1 33.891 239 118 12 67 268 164 399 3.95e-12 68.2
Msa0245000 AT1G27180.5 29.577 213 144 6 49 258 781 990 4.21e-12 68.2
Msa0245000 AT1G27180.8 29.577 213 144 6 49 258 781 990 4.21e-12 68.2
Msa0245000 AT4G13920.1 30.542 203 130 9 65 258 79 279 5.14e-12 67.8
Msa0245000 AT1G27180.7 29.577 213 144 6 49 258 607 816 5.31e-12 67.8
Msa0245000 AT1G27180.6 29.577 213 144 6 49 258 607 816 5.31e-12 67.8
Msa0245000 AT1G27180.4 29.577 213 144 6 49 258 607 816 5.31e-12 67.8
Msa0245000 AT3G24240.1 35.484 217 130 10 56 265 289 502 6.14e-12 67.8
Msa0245000 AT3G11010.5 32.189 233 145 10 39 265 61 286 1.69e-11 66.2
Msa0245000 AT3G11010.3 32.189 233 145 10 39 265 124 349 1.70e-11 66.2
Msa0245000 AT3G47570.1 32.927 246 154 11 91 328 338 580 2.85e-11 65.5
Msa0245000 AT5G61480.1 31.556 225 143 10 51 267 114 335 4.25e-11 65.1
Msa0245000 AT1G75640.1 29.126 206 136 9 70 267 293 496 5.73e-11 64.7
Msa0245000 AT5G62230.2 32.864 213 134 9 67 273 73 282 7.01e-11 64.3
Msa0245000 AT4G20140.1 30.435 230 125 11 70 267 580 806 7.43e-11 64.3
Msa0245000 AT5G17680.1 26.587 252 145 6 45 260 761 1008 8.56e-11 63.9
Msa0245000 AT5G17680.2 26.587 252 145 6 45 260 762 1009 8.87e-11 63.9
Msa0245000 AT2G24130.1 34.247 219 134 10 57 267 337 553 9.33e-11 63.9
Msa0245000 AT2G24130.2 34.247 219 134 10 57 267 352 568 9.37e-11 63.9

Find 111 sgRNAs with CRISPR-Local

Find 198 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAATTCTGACTCACATTTA+AGG 0.161777 2_2:-23891635 None:intergenic
CACCATCCACCATCTCTTCT+TGG 0.191377 2_2:-23891896 None:intergenic
TTCCCTCCACGCTTAGCTTT+TGG 0.217550 2_2:-23891774 None:intergenic
GTTGAGATTCAGTTCTTCTA+AGG 0.278250 2_2:-23891416 None:intergenic
TAAATGTGAGTCAGAATTTC+CGG 0.281058 2_2:+23891638 Msa0245000:CDS
TTTGTCTCCCTCTTCCTTGT+TGG 0.292650 2_2:-23888706 None:intergenic
CAGCAACCATTGAGAATTGC+AGG 0.306044 2_2:+23889686 Msa0245000:CDS
AGAGAGACAACCAATGGAAT+TGG 0.315520 2_2:-23889617 None:intergenic
CAATGGTTGCTGGGAGGGAT+TGG 0.332249 2_2:-23889675 None:intergenic
TCTTGCTTCACCAGGCTTCA+TGG 0.336493 2_2:+23892008 Msa0245000:CDS
CGACGCCGACGGAGGAAGAT+AGG 0.344483 2_2:+23888743 Msa0245000:CDS
CTTGCTTCACCAGGCTTCAT+GGG 0.358409 2_2:+23892009 Msa0245000:CDS
TCCACTGGAGGTGGTGGAGC+AGG 0.362764 2_2:+23891813 Msa0245000:CDS
CTGCAATTCTCAATGGTTGC+TGG 0.367867 2_2:-23889685 None:intergenic
CCCGCTCAGTTCGCCTCCAC+TGG 0.372267 2_2:+23891798 Msa0245000:CDS
CTCAAAGCTTTGAATGTCTC+TGG 0.381783 2_2:+23889643 Msa0245000:CDS
TCTTCTTGGTTGGGATATGA+TGG 0.383328 2_2:-23891882 None:intergenic
GCTAAATCAAGAGAAAGTTT+AGG 0.388906 2_2:-23888808 None:intergenic
CTCTCACATGATGACTCTTA+TGG 0.393115 2_2:+23891543 Msa0245000:CDS
ATCCACCATCTCTTCTTGGT+TGG 0.393964 2_2:-23891892 None:intergenic
ATTATTAAACTTGGAGATGT+TGG 0.403539 2_2:+23889564 Msa0245000:CDS
GGACTTGCATGGCATGTCCT+TGG 0.409480 2_2:+23888777 Msa0245000:CDS
ACTTATCATGTCTCCATTGA+AGG 0.410083 2_2:-23891929 None:intergenic
TGGTGAAGCAAGACCATTCA+TGG 0.416329 2_2:-23891998 None:intergenic
TTCGCCTCCACTGGAGGTGG+TGG 0.417968 2_2:+23891807 Msa0245000:CDS
TCCACCATCTCTTCTTGGTT+GGG 0.419645 2_2:-23891891 None:intergenic
GAGTCAGAATTTCCGGTACT+TGG 0.428594 2_2:+23891645 Msa0245000:CDS
ACGAGGTTCTCAAGGTCGTC+GGG 0.432286 2_2:-23891601 None:intergenic
AGCTCAAAGCCTATTGTGTC+TGG 0.435730 2_2:-23891460 None:intergenic
TGGGAAACGATCAGAACATA+AGG 0.438511 2_2:+23891951 Msa0245000:CDS
GAGAGACAACCAATGGAATT+GGG 0.443595 2_2:-23889616 None:intergenic
CTTCACCAGGCTTCATGGGG+AGG 0.443666 2_2:+23892013 Msa0245000:CDS
TTCAATGGAGACATGATAAG+TGG 0.444496 2_2:+23891931 Msa0245000:CDS
AGCAAACTTCCAGACACAAT+AGG 0.451995 2_2:+23891451 Msa0245000:CDS
ATTCTCAATGGTTGCTGGGA+GGG 0.461311 2_2:-23889680 None:intergenic
AACGGCAAGATTATTAAACT+TGG 0.472327 2_2:+23889555 Msa0245000:CDS
CCACTGGAGGTGGTGGAGCA+GGG 0.480559 2_2:+23891814 Msa0245000:CDS
TGGAGGCATCCGATGGAGTC+GGG 0.485570 2_2:-23891745 None:intergenic
AGGAAGACACACAAGTTTGT+TGG 0.489389 2_2:-23891512 None:intergenic
GACAACCTCCCCATGAAGCC+TGG 0.490735 2_2:-23892018 None:intergenic
TGCAATTCTCAATGGTTGCT+GGG 0.491491 2_2:-23889684 None:intergenic
CCAGTGGAGGCGAACTGAGC+GGG 0.492506 2_2:-23891798 None:intergenic
CTGGAGGCATCCGATGGAGT+CGG 0.492595 2_2:-23891746 None:intergenic
GAACATACCGGAGTCATTAA+CGG 0.493750 2_2:+23889537 Msa0245000:intron
GTGAAATGTGGGACCTTCAA+TGG 0.499860 2_2:+23891916 Msa0245000:CDS
CAGTTCGCCTCCACTGGAGG+TGG 0.507058 2_2:+23891804 Msa0245000:CDS
AATAAACCTGCAATTCTCAA+TGG 0.511036 2_2:-23889692 None:intergenic
TCAACTTAAGGAATTACGAC+GGG 0.513441 2_2:-23892061 None:intergenic
CAAGATTGACGAGGTTCTCA+AGG 0.516518 2_2:-23891609 None:intergenic
TCATCAGATGACAATGAAGA+TGG 0.519446 2_2:+23888675 Msa0245000:CDS
CTTCATTATCAACCCATGAA+TGG 0.521629 2_2:+23891985 Msa0245000:CDS
AGATCCCTACCCAATTCCAT+TGG 0.522866 2_2:+23889607 Msa0245000:CDS
ATGAATGGTCTTGCTTCACC+AGG 0.524055 2_2:+23892000 Msa0245000:CDS
TGCTCCACCACCTCCAGTGG+AGG 0.525602 2_2:-23891811 None:intergenic
GAGCTTCTGGAGGCATCCGA+TGG 0.527574 2_2:-23891752 None:intergenic
CAACAGTAGTCCTATGGAGT+AGG 0.528656 2_2:-23891677 None:intergenic
CCCTGCTCCACCACCTCCAG+TGG 0.531840 2_2:-23891814 None:intergenic
GAGTCATTGCCCTACTCCAT+AGG 0.539286 2_2:+23891667 Msa0245000:CDS
GGAAGATAGGGTTCATGTTG+TGG 0.545097 2_2:+23888756 Msa0245000:CDS
CTCCAAAAGCTAAGCGTGGA+GGG 0.545563 2_2:+23891772 Msa0245000:CDS
TAGGGACAACAGTAGTCCTA+TGG 0.545811 2_2:-23891683 None:intergenic
ATCATATGCCAACAAGGAAG+AGG 0.547319 2_2:+23888698 Msa0245000:CDS
AATTACGACGGGGTGAGAAG+AGG 0.549684 2_2:-23892050 None:intergenic
ACGTACGTTTGAATTGCCTC+AGG 0.550757 2_2:+23891572 Msa0245000:CDS
GACGAGGTTCTCAAGGTCGT+CGG 0.550775 2_2:-23891602 None:intergenic
TTGCTTCACCAGGCTTCATG+GGG 0.551908 2_2:+23892010 Msa0245000:CDS
GACGCCGACGGAGGAAGATA+GGG 0.554013 2_2:+23888744 Msa0245000:CDS
CTATCTTCCTCCGTCGGCGT+CGG 0.555118 2_2:-23888742 None:intergenic
AAGTTTAGGAAGTGAGTCCA+AGG 0.558762 2_2:-23888794 None:intergenic
GACAACCAATGGAATTGGGT+AGG 0.561937 2_2:-23889612 None:intergenic
GTTCATGTTGTGGACTTGCA+TGG 0.567190 2_2:+23888766 Msa0245000:CDS
AGCTAACGTCTAGTTCAACT+AGG 0.568083 2_2:-23891702 None:intergenic
AAAACATTGCCCGACTCCAT+CGG 0.568332 2_2:+23891736 Msa0245000:CDS
GCTCAGTTCGCCTCCACTGG+AGG 0.570036 2_2:+23891801 Msa0245000:CDS
AGATGGTGGATGGTGAAATG+TGG 0.570372 2_2:+23891904 Msa0245000:CDS
AACAGTAGTCCTATGGAGTA+GGG 0.572641 2_2:-23891676 None:intergenic
ATCATGTGAGAGGTGGAGCT+AGG 0.579817 2_2:-23891532 None:intergenic
ACAACCAATGGAATTGGGTA+GGG 0.580388 2_2:-23889611 None:intergenic
GACGACGTCGCCGACGCCGA+CGG 0.580655 2_2:+23888732 Msa0245000:CDS
GGGAAACGATCAGAACATAA+GGG 0.581219 2_2:+23891952 Msa0245000:CDS
TTTGTCTAACAACAATCTCC+AGG 0.584234 2_2:+23888849 Msa0245000:CDS
AATCTTGCCGTTAATGACTC+CGG 0.589085 2_2:-23889544 None:intergenic
GAGTTTAGAGAGACAACCAA+TGG 0.592305 2_2:-23889623 None:intergenic
CGGGGTGAGAAGAGGCGAAG+AGG 0.596579 2_2:-23892042 None:intergenic
AGGGCAATGACTCCAAGTAC+CGG 0.604878 2_2:-23891657 None:intergenic
GCTCCAAAAGCTAAGCGTGG+AGG 0.605699 2_2:+23891771 Msa0245000:CDS
TCATATGCCAACAAGGAAGA+GGG 0.605953 2_2:+23888699 Msa0245000:CDS
ATATCCCAACCAAGAAGAGA+TGG 0.606320 2_2:+23891887 Msa0245000:CDS
AACCAAGAAGAGATGGTGGA+TGG 0.610943 2_2:+23891894 Msa0245000:CDS
AATTGGGTAGGGATCTAAGC+TGG 0.619182 2_2:-23889600 None:intergenic
TCAATGGAGACATGATAAGT+GGG 0.620791 2_2:+23891932 Msa0245000:CDS
AGATGGATCATATGCCAACA+AGG 0.620987 2_2:+23888692 Msa0245000:CDS
AAGAGTCATCATGTGAGAGG+TGG 0.622685 2_2:-23891539 None:intergenic
GGGTAGGGATCTAAGCTGGT+TGG 0.623614 2_2:-23889596 None:intergenic
GACGTCGCCGACGCCGACGG+AGG 0.630661 2_2:+23888735 Msa0245000:CDS
TCCCAACCAAGAAGAGATGG+TGG 0.632111 2_2:+23891890 Msa0245000:CDS
GCTAACGTCTAGTTCAACTA+GGG 0.636021 2_2:-23891701 None:intergenic
CTCAACTTAAGGAATTACGA+CGG 0.637737 2_2:-23892062 None:intergenic
GGTCGTCGGGGAGACTCCTG+AGG 0.642718 2_2:-23891588 None:intergenic
GGTGAAGCAAGACCATTCAT+GGG 0.642951 2_2:-23891997 None:intergenic
CATAAGAGTCATCATGTGAG+AGG 0.643496 2_2:-23891542 None:intergenic
GAAGCTCCAAAAGCTAAGCG+TGG 0.653198 2_2:+23891768 Msa0245000:CDS
GATGGTGGATGGTGAAATGT+GGG 0.654566 2_2:+23891905 Msa0245000:CDS
AATTCTCAATGGTTGCTGGG+AGG 0.654863 2_2:-23889681 None:intergenic
TCCAGTGGAGGCGAACTGAG+CGG 0.662649 2_2:-23891799 None:intergenic
GGTGGAGCAGGGATTGCATG+TGG 0.678013 2_2:+23891825 Msa0245000:CDS
CTATATATAACAGAACATAC+CGG 0.683092 2_2:+23889525 Msa0245000:intron
CGAGGTTCTCAAGGTCGTCG+GGG 0.683613 2_2:-23891600 None:intergenic
TGAACCCTATCTTCCTCCGT+CGG 0.693937 2_2:-23888748 None:intergenic
TTAAGGTTTCAAGATTGACG+AGG 0.694244 2_2:-23891618 None:intergenic
CAACTTAAGGAATTACGACG+GGG 0.695800 2_2:-23892060 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATTAGATGTAATATAA+CGG + chr2_2:23889265-23889284 Msa0245000:intron 10.0%
!! TGTTATATAAATTATTTGAT+AGG + chr2_2:23889488-23889507 Msa0245000:intron 10.0%
!!! TAGTTTTAAAAGTAATAATT+TGG + chr2_2:23889948-23889967 Msa0245000:intron 10.0%
!!! TATTCTTGTTTTTTTTTTTT+AGG + chr2_2:23890983-23891002 Msa0245000:intron 10.0%
!!! ATTCTTGTTTTTTTTTTTTA+GGG + chr2_2:23890984-23891003 Msa0245000:intron 10.0%
!!! AAAGATTATTCTATTTGTTA+TGG - chr2_2:23889096-23889115 None:intergenic 15.0%
!! AATAGTTAATAAGGATAATT+TGG - chr2_2:23889925-23889944 None:intergenic 15.0%
!! AAATTATCCTTATTAACTAT+TGG + chr2_2:23889924-23889943 Msa0245000:intron 15.0%
!!! AATTTGTTAATATAAGTCTT+TGG - chr2_2:23890683-23890702 None:intergenic 15.0%
!! TATTAACAAATTACATTTGT+AGG + chr2_2:23890691-23890710 Msa0245000:intron 15.0%
!!! TTCTTGTTTTTTTTTTTTAG+GGG + chr2_2:23890985-23891004 Msa0245000:intron 15.0%
!! AAAAAATATATTTAATTGCC+TGG + chr2_2:23891088-23891107 Msa0245000:intron 15.0%
!!! CCAATTTTATCATATAATAA+TGG - chr2_2:23891266-23891285 None:intergenic 15.0%
!! CCATTATTATATGATAAAAT+TGG + chr2_2:23891263-23891282 Msa0245000:intron 15.0%
!!! AGTACAATGCAATTTTATTA+AGG + chr2_2:23888909-23888928 Msa0245000:intron 20.0%
!! AATAGAATAATCTTTGTTGA+AGG + chr2_2:23889101-23889120 Msa0245000:intron 20.0%
!! ATAGAATAATCTTTGTTGAA+GGG + chr2_2:23889102-23889121 Msa0245000:intron 20.0%
!!! AAACAACACTCATTTTAAAA+CGG - chr2_2:23889842-23889861 None:intergenic 20.0%
!!! TTTAAAATGAGTGTTGTTTT+AGG + chr2_2:23889843-23889862 Msa0245000:intron 20.0%
!! AAACTAACCAATAGTTAATA+AGG - chr2_2:23889934-23889953 None:intergenic 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG + chr2_2:23890011-23890030 Msa0245000:intron 20.0%
!!! TTGCATAACTTTTTTTTTAG+TGG + chr2_2:23890258-23890277 Msa0245000:intron 20.0%
!!! ATAACTTTTTTTTTAGTGGT+CGG + chr2_2:23890262-23890281 Msa0245000:intron 20.0%
!! AAACAAGAATAAAAGTCTAT+CGG - chr2_2:23890974-23890993 None:intergenic 20.0%
!! TTATTCAATCAATATCTAGT+TGG - chr2_2:23891200-23891219 None:intergenic 20.0%
! AATTGTGAATTTGTCAAACT+TGG - chr2_2:23888883-23888902 None:intergenic 25.0%
! TCCAAATCTTAAAATCATAG+TGG + chr2_2:23889061-23889080 Msa0245000:intron 25.0%
!!! ACCACTATGATTTTAAGATT+TGG - chr2_2:23889065-23889084 None:intergenic 25.0%
!!! GGATTTTTTTGATAAGTATC+TGG + chr2_2:23889150-23889169 Msa0245000:intron 25.0%
! CTATATATAACAGAACATAC+CGG + chr2_2:23889525-23889544 Msa0245000:intron 25.0%
! ATTATTAAACTTGGAGATGT+TGG + chr2_2:23889564-23889583 Msa0245000:CDS 25.0%
!!! ACATGTTTTTAGTTGTGTAT+AGG + chr2_2:23890147-23890166 Msa0245000:intron 25.0%
!!! TAACTTTTTTTTTAGTGGTC+GGG + chr2_2:23890263-23890282 Msa0245000:intron 25.0%
!!! ATGAATAGATAACTTGTTTG+AGG - chr2_2:23890613-23890632 None:intergenic 25.0%
! TTTATTTGCATGACATCATT+AGG - chr2_2:23890765-23890784 None:intergenic 25.0%
! ACAAATATTGAACCAGAATT+GGG - chr2_2:23890886-23890905 None:intergenic 25.0%
! AACAAATATTGAACCAGAAT+TGG - chr2_2:23890887-23890906 None:intergenic 25.0%
! AAAAACGAGAAATTCATGAT+GGG - chr2_2:23891053-23891072 None:intergenic 25.0%
! TAAAAACGAGAAATTCATGA+TGG - chr2_2:23891054-23891073 None:intergenic 25.0%
! TGATTAGAATTGAAATTTCC+AGG - chr2_2:23891109-23891128 None:intergenic 25.0%
!!! CAATATTGATTTTGTGATGT+TGG - chr2_2:23891145-23891164 None:intergenic 25.0%
GCTAAATCAAGAGAAAGTTT+AGG - chr2_2:23888811-23888830 None:intergenic 30.0%
! TGGATCTAAATCCATGATTT+TGG - chr2_2:23889045-23889064 None:intergenic 30.0%
AATCTTTGTTGAAGGGAAAA+TGG + chr2_2:23889109-23889128 Msa0245000:intron 30.0%
AACGGCAAGATTATTAAACT+TGG + chr2_2:23889555-23889574 Msa0245000:CDS 30.0%
AATAAACCTGCAATTCTCAA+TGG - chr2_2:23889695-23889714 None:intergenic 30.0%
ACCCATATCAAGTTAATGTT+AGG + chr2_2:23889799-23889818 Msa0245000:intron 30.0%
! CCCTAACATTAACTTGATAT+GGG - chr2_2:23889803-23889822 None:intergenic 30.0%
CCCATATCAAGTTAATGTTA+GGG + chr2_2:23889800-23889819 Msa0245000:intron 30.0%
! TCCCTAACATTAACTTGATA+TGG - chr2_2:23889804-23889823 None:intergenic 30.0%
!!! AACACTCATTTTAAAACGGA+TGG - chr2_2:23889838-23889857 None:intergenic 30.0%
! TGTGCAGAGTATTAATTAGA+GGG + chr2_2:23889977-23889996 Msa0245000:intron 30.0%
!! AAGCAACACTCATTTTGAAA+CGG + chr2_2:23890077-23890096 Msa0245000:intron 30.0%
GCAAAACACAATAATGAACA+TGG - chr2_2:23890242-23890261 None:intergenic 30.0%
!!! AACTTTTTTTTTAGTGGTCG+GGG + chr2_2:23890264-23890283 Msa0245000:intron 30.0%
GACAATGCATAATATATGCA+AGG - chr2_2:23890303-23890322 None:intergenic 30.0%
!! ATATAAGTCTTTGGTTTGCA+TGG - chr2_2:23890674-23890693 None:intergenic 30.0%
GAACTAAGCATGATTAAATC+AGG + chr2_2:23890947-23890966 Msa0245000:intron 30.0%
AAAACGAGAAATTCATGATG+GGG - chr2_2:23891052-23891071 None:intergenic 30.0%
TCAATCAATATCTAGTTGGT+AGG - chr2_2:23891196-23891215 None:intergenic 30.0%
GAAATTCTGACTCACATTTA+AGG - chr2_2:23891638-23891657 None:intergenic 30.0%
TAAATGTGAGTCAGAATTTC+CGG + chr2_2:23891638-23891657 Msa0245000:CDS 30.0%
TCATCAGATGACAATGAAGA+TGG + chr2_2:23888675-23888694 Msa0245000:CDS 35.0%
TTTGTCTAACAACAATCTCC+AGG + chr2_2:23888849-23888868 Msa0245000:CDS 35.0%
TCATGATGAGATCAATAGCT+AGG + chr2_2:23889000-23889019 Msa0245000:intron 35.0%
TGAGATCAATAGCTAGGAAT+AGG + chr2_2:23889006-23889025 Msa0245000:intron 35.0%
TGGCATAATCTTGTACTACT+TGG + chr2_2:23889129-23889148 Msa0245000:intron 35.0%
! GTGTGCAGAGTATTAATTAG+AGG + chr2_2:23889976-23889995 Msa0245000:intron 35.0%
!!! AACACTCATTTTGAAACGGA+TGG + chr2_2:23890081-23890100 Msa0245000:intron 35.0%
TGCATAATATATGCAAGGTC+CGG - chr2_2:23890298-23890317 None:intergenic 35.0%
TTGCATAACTATCTACCAAG+AGG + chr2_2:23890355-23890374 Msa0245000:intron 35.0%
TGCATAACTATCTACCAAGA+GGG + chr2_2:23890356-23890375 Msa0245000:intron 35.0%
!!! GTTTTTTTAAGTTCCCCTCT+TGG - chr2_2:23890373-23890392 None:intergenic 35.0%
AATATTGAACCAGAATTGGG+AGG - chr2_2:23890883-23890902 None:intergenic 35.0%
AAACGAGAAATTCATGATGG+GGG - chr2_2:23891051-23891070 None:intergenic 35.0%
! GATTTTGTGATGTTGGATCT+AGG - chr2_2:23891138-23891157 None:intergenic 35.0%
GTGCAATTGTGCTAAAAACT+TGG + chr2_2:23891287-23891306 Msa0245000:intron 35.0%
GTTGAGATTCAGTTCTTCTA+AGG - chr2_2:23891419-23891438 None:intergenic 35.0%
TTAAGGTTTCAAGATTGACG+AGG - chr2_2:23891621-23891640 None:intergenic 35.0%
ACTTATCATGTCTCCATTGA+AGG - chr2_2:23891932-23891951 None:intergenic 35.0%
TTCAATGGAGACATGATAAG+TGG + chr2_2:23891931-23891950 Msa0245000:CDS 35.0%
TCAATGGAGACATGATAAGT+GGG + chr2_2:23891932-23891951 Msa0245000:CDS 35.0%
CTTCATTATCAACCCATGAA+TGG + chr2_2:23891985-23892004 Msa0245000:CDS 35.0%
TCAACTTAAGGAATTACGAC+GGG - chr2_2:23892064-23892083 None:intergenic 35.0%
AGATGGATCATATGCCAACA+AGG + chr2_2:23888692-23888711 Msa0245000:CDS 40.0%
ATCATATGCCAACAAGGAAG+AGG + chr2_2:23888698-23888717 Msa0245000:CDS 40.0%
TCATATGCCAACAAGGAAGA+GGG + chr2_2:23888699-23888718 Msa0245000:CDS 40.0%
AAGTTTAGGAAGTGAGTCCA+AGG - chr2_2:23888797-23888816 None:intergenic 40.0%
TCAAACTTGGAGATTGTACC+TGG - chr2_2:23888870-23888889 None:intergenic 40.0%
GAACATACCGGAGTCATTAA+CGG + chr2_2:23889537-23889556 Msa0245000:intron 40.0%
AATCTTGCCGTTAATGACTC+CGG - chr2_2:23889547-23889566 None:intergenic 40.0%
ACAACCAATGGAATTGGGTA+GGG - chr2_2:23889614-23889633 None:intergenic 40.0%
GAGAGACAACCAATGGAATT+GGG - chr2_2:23889619-23889638 None:intergenic 40.0%
AGAGAGACAACCAATGGAAT+TGG - chr2_2:23889620-23889639 None:intergenic 40.0%
GAGTTTAGAGAGACAACCAA+TGG - chr2_2:23889626-23889645 None:intergenic 40.0%
!! CTCAAAGCTTTGAATGTCTC+TGG + chr2_2:23889643-23889662 Msa0245000:CDS 40.0%
TGCAATTCTCAATGGTTGCT+GGG - chr2_2:23889687-23889706 None:intergenic 40.0%
CATAATATATGCAAGGTCCG+GGG - chr2_2:23890296-23890315 None:intergenic 40.0%
GCATAATATATGCAAGGTCC+GGG - chr2_2:23890297-23890316 None:intergenic 40.0%
! TGAGCATAACTCAGTTGGTA+TGG - chr2_2:23890325-23890344 None:intergenic 40.0%
GCATAACTATCTACCAAGAG+GGG + chr2_2:23890357-23890376 Msa0245000:intron 40.0%
!! CTTTGGTTTGCATGGTACAT+GGG - chr2_2:23890666-23890685 None:intergenic 40.0%
!! TCTTTGGTTTGCATGGTACA+TGG - chr2_2:23890667-23890686 None:intergenic 40.0%
!! AATTGGGAGGTTTTGTCTGT+TGG - chr2_2:23890870-23890889 None:intergenic 40.0%
TATAAAGACGTGAAGCATGC+AGG - chr2_2:23891225-23891244 None:intergenic 40.0%
GCATGCTTCACGTCTTTATA+AGG + chr2_2:23891225-23891244 Msa0245000:intron 40.0%
!! ATTCAGTTCTTCTAAGGCTC+TGG - chr2_2:23891413-23891432 None:intergenic 40.0%
AGCAAACTTCCAGACACAAT+AGG + chr2_2:23891451-23891470 Msa0245000:CDS 40.0%
AGGAAGACACACAAGTTTGT+TGG - chr2_2:23891515-23891534 None:intergenic 40.0%
CATAAGAGTCATCATGTGAG+AGG - chr2_2:23891545-23891564 None:intergenic 40.0%
CTCTCACATGATGACTCTTA+TGG + chr2_2:23891543-23891562 Msa0245000:CDS 40.0%
AACAGTAGTCCTATGGAGTA+GGG - chr2_2:23891679-23891698 None:intergenic 40.0%
! GCTAACGTCTAGTTCAACTA+GGG - chr2_2:23891704-23891723 None:intergenic 40.0%
! AGCTAACGTCTAGTTCAACT+AGG - chr2_2:23891705-23891724 None:intergenic 40.0%
TCTTCTTGGTTGGGATATGA+TGG - chr2_2:23891885-23891904 None:intergenic 40.0%
ATATCCCAACCAAGAAGAGA+TGG + chr2_2:23891887-23891906 Msa0245000:CDS 40.0%
TGGGAAACGATCAGAACATA+AGG + chr2_2:23891951-23891970 Msa0245000:CDS 40.0%
GGGAAACGATCAGAACATAA+GGG + chr2_2:23891952-23891971 Msa0245000:CDS 40.0%
CAACTTAAGGAATTACGACG+GGG - chr2_2:23892063-23892082 None:intergenic 40.0%
TTTGTCTCCCTCTTCCTTGT+TGG - chr2_2:23888709-23888728 None:intergenic 45.0%
GGAAGATAGGGTTCATGTTG+TGG + chr2_2:23888756-23888775 Msa0245000:CDS 45.0%
GTTCATGTTGTGGACTTGCA+TGG + chr2_2:23888766-23888785 Msa0245000:CDS 45.0%
GCACTAGAGAGCCAAAATCA+TGG + chr2_2:23889031-23889050 Msa0245000:intron 45.0%
AATTGGGTAGGGATCTAAGC+TGG - chr2_2:23889603-23889622 None:intergenic 45.0%
AGATCCCTACCCAATTCCAT+TGG + chr2_2:23889607-23889626 Msa0245000:CDS 45.0%
GACAACCAATGGAATTGGGT+AGG - chr2_2:23889615-23889634 None:intergenic 45.0%
ATTCTCAATGGTTGCTGGGA+GGG - chr2_2:23889683-23889702 None:intergenic 45.0%
AATTCTCAATGGTTGCTGGG+AGG - chr2_2:23889684-23889703 None:intergenic 45.0%
CTGCAATTCTCAATGGTTGC+TGG - chr2_2:23889688-23889707 None:intergenic 45.0%
CAGCAACCATTGAGAATTGC+AGG + chr2_2:23889686-23889705 Msa0245000:CDS 45.0%
CAGACAAAACCTCCCAATTC+TGG + chr2_2:23890871-23890890 Msa0245000:intron 45.0%
AGCTCAAAGCCTATTGTGTC+TGG - chr2_2:23891463-23891482 None:intergenic 45.0%
AAGAGTCATCATGTGAGAGG+TGG - chr2_2:23891542-23891561 None:intergenic 45.0%
ACGTACGTTTGAATTGCCTC+AGG + chr2_2:23891572-23891591 Msa0245000:CDS 45.0%
CAAGATTGACGAGGTTCTCA+AGG - chr2_2:23891612-23891631 None:intergenic 45.0%
GAGTCAGAATTTCCGGTACT+TGG + chr2_2:23891645-23891664 Msa0245000:CDS 45.0%
CAACAGTAGTCCTATGGAGT+AGG - chr2_2:23891680-23891699 None:intergenic 45.0%
TAGGGACAACAGTAGTCCTA+TGG - chr2_2:23891686-23891705 None:intergenic 45.0%
AAAACATTGCCCGACTCCAT+CGG + chr2_2:23891736-23891755 Msa0245000:CDS 45.0%
!!! TTAGCTTTTGGAGCTTCTGG+AGG - chr2_2:23891765-23891784 None:intergenic 45.0%
TCCACCATCTCTTCTTGGTT+GGG - chr2_2:23891894-23891913 None:intergenic 45.0%
ATCCACCATCTCTTCTTGGT+TGG - chr2_2:23891895-23891914 None:intergenic 45.0%
!! AACCAAGAAGAGATGGTGGA+TGG + chr2_2:23891894-23891913 Msa0245000:CDS 45.0%
!! AGATGGTGGATGGTGAAATG+TGG + chr2_2:23891904-23891923 Msa0245000:CDS 45.0%
!! GATGGTGGATGGTGAAATGT+GGG + chr2_2:23891905-23891924 Msa0245000:CDS 45.0%
GTGAAATGTGGGACCTTCAA+TGG + chr2_2:23891916-23891935 Msa0245000:CDS 45.0%
GGTGAAGCAAGACCATTCAT+GGG - chr2_2:23892000-23892019 None:intergenic 45.0%
TGGTGAAGCAAGACCATTCA+TGG - chr2_2:23892001-23892020 None:intergenic 45.0%
! ATGAATGGTCTTGCTTCACC+AGG + chr2_2:23892000-23892019 Msa0245000:CDS 45.0%
!!! TTTATTAATATTTTAAAAAC+TGG + chr2_2:23890835-23890854 Msa0245000:intron 5.0%
TGAACCCTATCTTCCTCCGT+CGG - chr2_2:23888751-23888770 None:intergenic 50.0%
CCTCGTGAGCATAACTCAGT+TGG - chr2_2:23890330-23890349 None:intergenic 50.0%
CCAACTGAGTTATGCTCACG+AGG + chr2_2:23890327-23890346 Msa0245000:intron 50.0%
!! CAGTTCTTCTAAGGCTCTGG+TGG - chr2_2:23891410-23891429 None:intergenic 50.0%
ATCATGTGAGAGGTGGAGCT+AGG - chr2_2:23891535-23891554 None:intergenic 50.0%
AGGGCAATGACTCCAAGTAC+CGG - chr2_2:23891660-23891679 None:intergenic 50.0%
GAGTCATTGCCCTACTCCAT+AGG + chr2_2:23891667-23891686 Msa0245000:CDS 50.0%
!!! CGCTTAGCTTTTGGAGCTTC+TGG - chr2_2:23891768-23891787 None:intergenic 50.0%
GAAGCTCCAAAAGCTAAGCG+TGG + chr2_2:23891768-23891787 Msa0245000:CDS 50.0%
CTCCAAAAGCTAAGCGTGGA+GGG + chr2_2:23891772-23891791 Msa0245000:CDS 50.0%
!! TTCCCTCCACGCTTAGCTTT+TGG - chr2_2:23891777-23891796 None:intergenic 50.0%
TCCCAACCAAGAAGAGATGG+TGG + chr2_2:23891890-23891909 Msa0245000:CDS 50.0%
CACCATCCACCATCTCTTCT+TGG - chr2_2:23891899-23891918 None:intergenic 50.0%
TCTTGCTTCACCAGGCTTCA+TGG + chr2_2:23892008-23892027 Msa0245000:CDS 50.0%
CTTGCTTCACCAGGCTTCAT+GGG + chr2_2:23892009-23892028 Msa0245000:CDS 50.0%
TTGCTTCACCAGGCTTCATG+GGG + chr2_2:23892010-23892029 Msa0245000:CDS 50.0%
AATTACGACGGGGTGAGAAG+AGG - chr2_2:23892053-23892072 None:intergenic 50.0%
GGACTTGCATGGCATGTCCT+TGG + chr2_2:23888777-23888796 Msa0245000:CDS 55.0%
GGGTAGGGATCTAAGCTGGT+TGG - chr2_2:23889599-23889618 None:intergenic 55.0%
CAATGGTTGCTGGGAGGGAT+TGG - chr2_2:23889678-23889697 None:intergenic 55.0%
ACGAGGTTCTCAAGGTCGTC+GGG - chr2_2:23891604-23891623 None:intergenic 55.0%
GACGAGGTTCTCAAGGTCGT+CGG - chr2_2:23891605-23891624 None:intergenic 55.0%
GCTCCAAAAGCTAAGCGTGG+AGG + chr2_2:23891771-23891790 Msa0245000:CDS 55.0%
CTATCTTCCTCCGTCGGCGT+CGG - chr2_2:23888745-23888764 None:intergenic 60.0%
GACGCCGACGGAGGAAGATA+GGG + chr2_2:23888744-23888763 Msa0245000:CDS 60.0%
AGTGGTCGGGGTTTGAACCC+CGG + chr2_2:23890276-23890295 Msa0245000:intron 60.0%
CGAGGTTCTCAAGGTCGTCG+GGG - chr2_2:23891603-23891622 None:intergenic 60.0%
!! TGGAGGCATCCGATGGAGTC+GGG - chr2_2:23891748-23891767 None:intergenic 60.0%
!! CTGGAGGCATCCGATGGAGT+CGG - chr2_2:23891749-23891768 None:intergenic 60.0%
!! GAGCTTCTGGAGGCATCCGA+TGG - chr2_2:23891755-23891774 None:intergenic 60.0%
TCCAGTGGAGGCGAACTGAG+CGG - chr2_2:23891802-23891821 None:intergenic 60.0%
GGTGGAGCAGGGATTGCATG+TGG + chr2_2:23891825-23891844 Msa0245000:CDS 60.0%
CTTCACCAGGCTTCATGGGG+AGG + chr2_2:23892013-23892032 Msa0245000:CDS 60.0%
GACAACCTCCCCATGAAGCC+TGG - chr2_2:23892021-23892040 None:intergenic 60.0%
CGACGCCGACGGAGGAAGAT+AGG + chr2_2:23888743-23888762 Msa0245000:CDS 65.0%
CCAGTGGAGGCGAACTGAGC+GGG - chr2_2:23891801-23891820 None:intergenic 65.0%
GCTCAGTTCGCCTCCACTGG+AGG + chr2_2:23891801-23891820 Msa0245000:CDS 65.0%
CAGTTCGCCTCCACTGGAGG+TGG + chr2_2:23891804-23891823 Msa0245000:CDS 65.0%
TTCGCCTCCACTGGAGGTGG+TGG + chr2_2:23891807-23891826 Msa0245000:CDS 65.0%
TGCTCCACCACCTCCAGTGG+AGG - chr2_2:23891814-23891833 None:intergenic 65.0%
!! TCCACTGGAGGTGGTGGAGC+AGG + chr2_2:23891813-23891832 Msa0245000:CDS 65.0%
!! CCACTGGAGGTGGTGGAGCA+GGG + chr2_2:23891814-23891833 Msa0245000:CDS 65.0%
CGGGGTGAGAAGAGGCGAAG+AGG - chr2_2:23892045-23892064 None:intergenic 65.0%
GGTCGTCGGGGAGACTCCTG+AGG - chr2_2:23891591-23891610 None:intergenic 70.0%
CCCGCTCAGTTCGCCTCCAC+TGG + chr2_2:23891798-23891817 Msa0245000:CDS 70.0%
CCCTGCTCCACCACCTCCAG+TGG - chr2_2:23891817-23891836 None:intergenic 70.0%
GACGACGTCGCCGACGCCGA+CGG + chr2_2:23888732-23888751 Msa0245000:CDS 75.0%
! GACGTCGCCGACGCCGACGG+AGG + chr2_2:23888735-23888754 Msa0245000:CDS 80.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 23888637 23892083 23888637 ID=Msa0245000;Name=Msa0245000
chr2_2 mRNA 23888637 23892083 23888637 ID=Msa0245000-mRNA-1;Parent=Msa0245000;Name=Msa0245000-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|1|1|3|0|357
chr2_2 exon 23888637 23888870 23888637 ID=Msa0245000-mRNA-1:exon:9214;Parent=Msa0245000-mRNA-1
chr2_2 exon 23889538 23889707 23889538 ID=Msa0245000-mRNA-1:exon:9215;Parent=Msa0245000-mRNA-1
chr2_2 exon 23891414 23892083 23891414 ID=Msa0245000-mRNA-1:exon:9216;Parent=Msa0245000-mRNA-1
chr2_2 CDS 23888637 23888870 23888637 ID=Msa0245000-mRNA-1:cds;Parent=Msa0245000-mRNA-1
chr2_2 CDS 23889538 23889707 23889538 ID=Msa0245000-mRNA-1:cds;Parent=Msa0245000-mRNA-1
chr2_2 CDS 23891414 23892083 23891414 ID=Msa0245000-mRNA-1:cds;Parent=Msa0245000-mRNA-1
Gene Sequence

>Msa0245000

ATGTATGAATTGCAACAATTTCAGTTTCTTCATCAAGATCATCAGATGACAATGAAGATGGATCATATGCCAACAAGGAAGAGGGAGACAAAAAAGACGACGTCGCCGACGCCGACGGAGGAAGATAGGGTTCATGTTGTGGACTTGCATGGCATGTCCTTGGACTCACTTCCTAAACTTTCTCTTGATTTAGCTATTATTTGCAAGCTCAATTTGTCTAACAACAATCTCCAGAACATACCGGAGTCATTAACGGCAAGATTATTAAACTTGGAGATGTTGGACGTGCACTCCAACCAGCTTAGATCCCTACCCAATTCCATTGGTTGTCTCTCTAAACTCAAAGCTTTGAATGTCTCTGGAAACTTCATCCAATCCCTCCCAGCAACCATTGAGAATTGCAGAGCCTTAGAAGAACTGAATCTCAACTTCAACATGTTGAGCAAACTTCCAGACACAATAGGCTTTGAGCTCGTAAAACTCAAGAAGCTTGCAGTTAACTCCAACAAACTTGTGTGTCTTCCTAGCTCCACCTCTCACATGATGACTCTTATGGTTCTCGACGTACGTTTGAATTGCCTCAGGAGTCTCCCCGACGACCTTGAGAACCTCGTCAATCTTGAAACCTTAAATGTGAGTCAGAATTTCCGGTACTTGGAGTCATTGCCCTACTCCATAGGACTACTGTTGTCCCTAGTTGAACTAGACGTTAGCTACAACAATATCAAAACATTGCCCGACTCCATCGGATGCCTCCAGAAGCTCCAAAAGCTAAGCGTGGAGGGAAACCCGCTCAGTTCGCCTCCACTGGAGGTGGTGGAGCAGGGATTGCATGTGGTGAAAGAGTACATGTGTAACAAAATGAACTCAAGCCATCATATCCCAACCAAGAAGAGATGGTGGATGGTGAAATGTGGGACCTTCAATGGAGACATGATAAGTGGGAAACGATCAGAACATAAGGGTTTCAACAAGCTTCATTATCAACCCATGAATGGTCTTGCTTCACCAGGCTTCATGGGGAGGTTGTCGCCTCTTCGCCTCTTCTCACCCCGTCGTAATTCCTTAAGTTGA
Protein sequence

>Msa0245000

MYELQQFQFLHQDHQMTMKMDHMPTRKRETKKTTSPTPTEEDRVHVVDLHGMSLDSLPKLSLDLAIICKLNLSNNNLQNIPESLTARLLNLEMLDVHSNQLRSLPNSIGCLSKLKALNVSGNFIQSLPATIENCRALEELNLNFNMLSKLPDTIGFELVKLKKLAVNSNKLVCLPSSTSHMMTLMVLDVRLNCLRSLPDDLENLVNLETLNVSQNFRYLESLPYSIGLLLSLVELDVSYNNIKTLPDSIGCLQKLQKLSVEGNPLSSPPLEVVEQGLHVVKEYMCNKMNSSHHIPTKKRWWMVKCGTFNGDMISGKRSEHKGFNKLHYQPMNGLASPGFMGRLSPLRLFSPRRNSLS*