AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0321750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0321750 MtrunA17_Chr2g0298891 94.350 354 17 1 1 354 1 351 0.0 652
Msa0321750 MtrunA17_Chr4g0071581 72.871 317 80 3 40 352 24 338 1.05e-160 453
Msa0321750 MtrunA17_Chr4g0071581 35.152 165 104 2 106 269 20 182 4.90e-13 69.3
Msa0321750 MtrunA17_Chr4g0058541 47.303 241 126 1 47 287 208 447 1.78e-58 197
Msa0321750 MtrunA17_Chr4g0058541 30.061 163 111 2 43 203 273 434 1.79e-11 65.1
Msa0321750 MtrunA17_Chr3g0116991 49.083 218 110 1 70 287 228 444 4.47e-55 188
Msa0321750 MtrunA17_Chr3g0116991 33.129 163 106 2 43 203 270 431 1.94e-12 68.2
Msa0321750 MtrunA17_Chr5g0412701 43.952 248 138 1 40 287 148 394 5.79e-50 173
Msa0321750 MtrunA17_Chr8g0370111 39.773 264 152 3 47 305 294 555 1.12e-42 155
Msa0321750 MtrunA17_Chr5g0407461 40.167 239 141 2 47 285 305 541 2.05e-42 155
Msa0321750 MtrunA17_Chr5g0407461 40.351 171 99 2 99 269 264 431 3.18e-27 112
Msa0321750 MtrunA17_Chr4g0054721 38.346 266 139 3 44 286 219 482 7.00e-41 150
Msa0321750 MtrunA17_Chr4g0054721 36.782 174 107 2 99 272 204 374 2.14e-22 98.2
Msa0321750 MtrunA17_Chr8g0357151 36.015 261 154 12 19 269 245 502 2.88e-18 86.7
Msa0321750 MtrunA17_Chr8g0357151 34.529 223 134 11 56 269 115 334 2.12e-12 68.6
Msa0321750 MtrunA17_Chr8g0363851 32.231 242 158 6 47 286 610 847 5.56e-16 79.7
Msa0321750 MtrunA17_Chr8g0363851 34.031 191 119 6 75 263 545 730 5.16e-13 70.5
Msa0321750 MtrunA17_Chr8g0363851 32.353 204 122 7 47 237 703 903 1.08e-11 66.2
Msa0321750 MtrunA17_Chr3g0086371 30.804 224 124 6 46 266 24 219 6.18e-16 77.0
Msa0321750 MtrunA17_Chr3g0086371 27.586 203 121 3 72 274 27 203 3.27e-14 72.0
Msa0321750 MtrunA17_Chr1g0165141 30.112 269 169 13 56 310 237 500 7.31e-16 79.3
Msa0321750 MtrunA17_Chr1g0165141 29.018 224 145 8 49 267 94 308 1.16e-12 69.3
Msa0321750 MtrunA17_Chr8g0357171 36.444 225 129 13 58 273 155 374 2.54e-15 77.4
Msa0321750 MtrunA17_Chr8g0357171 32.227 211 103 8 82 263 116 315 2.49e-11 65.1
Msa0321750 MtrunA17_Chr8g0357171 31.304 230 141 11 57 272 201 427 4.29e-11 64.3
Msa0321750 MtrunA17_Chr8g0363891 33.803 213 127 6 54 264 162 362 3.95e-15 76.6
Msa0321750 MtrunA17_Chr8g0363891 34.409 186 117 5 77 260 129 311 1.42e-13 72.0
Msa0321750 MtrunA17_Chr8g0363891 33.333 189 120 6 77 263 82 266 1.08e-11 66.2
Msa0321750 MtrunA17_Chr8g0363891 36.652 221 114 12 51 263 90 292 5.00e-11 64.3
Msa0321750 MtrunA17_Chr2g0317831 31.280 211 140 5 63 272 67 273 1.29e-14 75.1
Msa0321750 MtrunA17_Chr2g0317831 27.347 245 149 5 47 288 364 582 4.29e-13 70.5
Msa0321750 MtrunA17_Chr4g0012841 35.071 211 126 11 70 272 150 357 2.96e-14 74.3
Msa0321750 MtrunA17_Chr8g0357191 36.792 212 124 10 63 267 254 462 4.92e-14 73.6
Msa0321750 MtrunA17_Chr8g0357191 32.927 246 131 12 56 269 127 370 1.30e-13 72.4
Msa0321750 MtrunA17_Chr8g0357191 33.019 212 129 10 62 267 114 318 3.51e-11 64.7
Msa0321750 MtrunA17_Chr7g0215641 36.022 186 107 8 90 267 90 271 1.63e-13 72.0
Msa0321750 MtrunA17_Chr7g0232211 32.056 287 134 13 67 295 76 359 2.40e-13 71.6
Msa0321750 MtrunA17_Chr1g0165221 31.963 219 141 8 56 268 213 429 2.53e-13 71.2
Msa0321750 MtrunA17_Chr1g0165221 31.905 210 135 8 64 267 101 308 6.88e-12 67.0
Msa0321750 MtrunA17_Chr1g0203251 26.490 302 183 8 19 284 405 703 3.83e-13 70.9
Msa0321750 MtrunA17_Chr2g0304481 37.143 175 102 8 80 249 437 608 5.23e-13 70.5
Msa0321750 MtrunA17_Chr8g0362431 30.542 203 129 7 70 265 151 348 8.03e-13 69.7
Msa0321750 MtrunA17_Chr2g0296601 33.014 209 129 11 67 267 599 804 9.38e-13 69.7
Msa0321750 MtrunA17_Chr8g0363111 34.759 187 115 7 35 215 411 596 1.12e-12 69.3
Msa0321750 MtrunA17_Chr1g0190411 28.016 257 150 11 44 268 78 331 1.22e-12 69.3
Msa0321750 MtrunA17_Chr2g0296591 33.014 209 129 11 67 267 599 804 1.25e-12 69.3
Msa0321750 MtrunA17_Chr1g0165901 29.231 260 173 10 19 269 238 495 2.12e-12 68.6
Msa0321750 MtrunA17_Chr4g0073641 31.401 207 135 7 67 267 356 561 2.18e-12 68.6
Msa0321750 MtrunA17_Chr8g0354031 40.000 155 86 7 67 215 206 359 3.00e-12 68.2
Msa0321750 MtrunA17_Chr3g0105881 29.064 203 134 5 73 269 136 334 4.22e-12 67.0
Msa0321750 MtrunA17_Chr5g0446871 37.736 159 91 8 63 215 281 437 4.52e-12 67.4
Msa0321750 MtrunA17_Chr8g0362931 32.353 238 143 11 46 267 186 421 4.64e-12 67.4
Msa0321750 MtrunA17_Chr8g0362931 35.065 154 94 6 67 215 629 781 5.97e-11 63.9
Msa0321750 MtrunA17_Chr5g0439291 33.493 209 112 8 63 268 132 316 7.83e-12 66.6
Msa0321750 MtrunA17_Chr7g0232451 32.328 232 124 9 67 267 95 324 9.54e-12 66.6
Msa0321750 MtrunA17_Chr1g0165211 35.052 194 117 9 67 254 603 793 1.07e-11 66.2
Msa0321750 MtrunA17_Chr1g0165211 30.088 226 148 10 50 268 322 544 1.65e-11 65.9
Msa0321750 MtrunA17_Chr3g0128731 30.769 221 139 9 56 265 269 486 1.19e-11 66.2
Msa0321750 MtrunA17_Chr7g0232171 31.359 287 136 13 67 295 85 368 1.37e-11 66.2
Msa0321750 MtrunA17_Chr7g0217081 35.514 214 124 11 63 267 133 341 1.42e-11 65.9
Msa0321750 MtrunA17_Chr6g0487031 27.148 291 156 10 12 274 25 287 1.51e-11 65.9
Msa0321750 MtrunA17_Chr7g0232131 30.796 289 138 14 67 296 85 370 1.51e-11 65.9
Msa0321750 MtrunA17_Chr1g0176301 35.366 164 99 7 67 225 200 361 2.13e-11 65.5
Msa0321750 MtrunA17_Chr1g0165191 34.536 194 118 9 67 254 583 773 2.56e-11 65.1
Msa0321750 MtrunA17_Chr3g0089251 41.176 102 58 2 160 260 606 706 2.73e-11 65.1
Msa0321750 MtrunA17_Chr3g0089961 45.349 86 46 1 174 259 595 679 2.77e-11 65.1
Msa0321750 MtrunA17_Chr1g0165951 32.759 232 145 11 43 266 98 326 3.13e-11 65.1
Msa0321750 MtrunA17_Chr1g0204461 29.767 215 119 9 57 265 316 504 3.71e-11 64.7
Msa0321750 MtrunA17_Chr7g0215681 29.851 268 159 11 14 267 39 291 3.96e-11 64.3
Msa0321750 MtrunA17_Chr7g0241921 31.336 217 140 9 56 265 371 585 4.28e-11 64.3
Msa0321750 MtrunA17_Chr5g0436901 35.567 194 114 11 80 265 67 257 5.03e-11 63.5
Msa0321750 MtrunA17_Chr2g0315071 31.390 223 136 12 56 267 12 228 5.11e-11 63.9
Msa0321750 MtrunA17_Chr7g0244931 31.963 219 135 8 57 265 337 551 5.18e-11 64.3
Msa0321750 MtrunA17_Chr3g0089501 45.977 87 46 1 174 260 590 675 5.32e-11 64.3
Msa0321750 MtrunA17_Chr3g0089601 45.977 87 46 1 174 260 590 675 5.35e-11 64.3
Msa0321750 MtrunA17_Chr4g0035444 29.064 203 136 8 67 264 409 608 5.95e-11 63.9
Msa0321750 MtrunA17_Chr2g0314021 27.306 271 155 9 38 267 212 481 8.90e-11 63.5
Msa0321750 MtrunA17_Chr2g0314001 28.384 229 146 10 54 267 256 481 8.90e-11 63.5
Msa0321750 MtrunA17_Chr7g0215011 31.381 239 123 11 42 272 64 269 9.09e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0321750 AT2G19330.1 64.151 318 109 2 40 352 58 375 6.37e-139 400
Msa0321750 AT4G26050.1 61.846 325 114 5 37 351 49 373 1.17e-125 366
Msa0321750 AT4G29880.1 61.950 318 116 2 40 352 51 368 3.06e-125 365
Msa0321750 AT3G11330.1 49.180 244 123 1 44 287 200 442 9.94e-58 195
Msa0321750 AT3G11330.1 32.298 161 106 2 43 201 268 427 1.73e-11 65.9
Msa0321750 AT5G05850.1 48.400 250 128 1 41 290 203 451 8.62e-57 193
Msa0321750 AT3G26500.1 40.856 257 139 2 44 300 162 405 4.08e-51 177
Msa0321750 AT1G12970.1 40.467 257 140 2 44 300 163 406 1.04e-48 171
Msa0321750 AT1G12970.1 35.294 187 110 6 35 215 200 381 1.95e-11 65.5
Msa0321750 AT4G35470.1 38.168 262 157 3 47 305 274 533 2.54e-42 155
Msa0321750 AT4G35470.1 39.196 199 107 3 98 285 232 427 1.49e-22 99.4
Msa0321750 AT2G17440.1 39.924 263 152 3 47 305 258 518 4.15e-40 148
Msa0321750 AT4G35470.2 40.171 234 138 2 47 280 274 505 2.29e-39 147
Msa0321750 AT4G35470.2 39.196 199 107 3 98 285 232 427 1.82e-22 99.0
Msa0321750 AT3G15410.2 34.450 209 132 4 63 270 72 276 3.78e-18 86.3
Msa0321750 AT3G15410.2 34.783 207 130 4 67 272 53 255 6.87e-18 85.5
Msa0321750 AT3G15410.2 28.723 282 166 6 46 298 78 353 2.44e-16 80.9
Msa0321750 AT3G15410.2 28.879 232 132 5 83 288 363 587 9.08e-14 72.8
Msa0321750 AT3G15410.1 35.071 211 128 5 63 270 66 270 3.89e-18 86.3
Msa0321750 AT3G15410.1 34.783 207 130 4 67 272 47 249 6.94e-18 85.5
Msa0321750 AT3G15410.1 28.723 282 166 6 46 298 72 347 2.61e-16 80.5
Msa0321750 AT3G15410.1 28.879 232 132 5 83 288 357 581 1.02e-13 72.8
Msa0321750 AT2G30105.1 34.211 190 109 5 101 278 141 326 2.91e-16 79.7
Msa0321750 AT2G30105.1 41.964 112 63 2 70 181 207 316 7.69e-15 75.5
Msa0321750 AT2G34930.1 33.766 231 136 10 47 263 204 431 1.14e-14 75.9
Msa0321750 AT1G27170.1 29.817 218 123 6 67 258 767 980 2.66e-14 75.1
Msa0321750 AT4G28490.1 34.409 186 112 9 70 247 119 302 3.07e-14 74.7
Msa0321750 AT1G27170.2 30.415 217 123 5 67 258 767 980 3.24e-14 74.7
Msa0321750 AT5G07910.1 27.586 232 137 6 46 274 25 228 8.19e-14 71.2
Msa0321750 AT1G69550.1 39.171 217 121 11 53 262 920 1132 9.42e-14 73.2
Msa0321750 AT1G69550.1 38.660 194 110 9 75 262 871 1061 3.23e-13 71.6
Msa0321750 AT1G69550.1 38.739 222 124 11 47 260 985 1202 6.70e-13 70.5
Msa0321750 AT1G69550.1 30.888 259 146 9 35 262 733 989 2.16e-11 65.9
Msa0321750 AT1G69550.1 33.818 275 156 13 53 317 1016 1274 5.56e-11 64.7
Msa0321750 AT3G56370.1 33.721 258 134 11 42 265 69 323 1.54e-13 72.4
Msa0321750 AT3G56370.1 32.911 237 142 9 36 263 230 458 1.49e-12 69.3
Msa0321750 AT2G15080.1 33.632 223 132 12 53 267 104 318 1.71e-13 72.4
Msa0321750 AT2G15080.2 33.632 223 132 12 53 267 104 318 1.71e-13 72.4
Msa0321750 AT5G44700.1 33.766 231 141 11 44 265 266 493 2.99e-13 71.6
Msa0321750 AT5G44700.1 30.342 234 154 9 17 243 599 830 9.46e-12 67.0
Msa0321750 AT1G27180.1 29.577 213 144 6 49 258 945 1154 1.04e-12 70.1
Msa0321750 AT1G27180.1 30.500 200 133 6 64 260 913 1109 8.02e-11 64.3
Msa0321750 AT1G27180.2 29.577 213 144 6 49 258 783 992 1.14e-12 70.1
Msa0321750 AT1G27180.2 30.500 200 133 6 64 260 751 947 8.15e-11 64.3
Msa0321750 AT1G27180.3 29.577 213 144 6 49 258 783 992 1.18e-12 69.7
Msa0321750 AT1G27180.3 30.631 222 144 7 49 265 830 1046 1.64e-11 66.2
Msa0321750 AT1G27180.3 30.500 200 133 6 64 260 751 947 8.17e-11 63.9
Msa0321750 AT1G27180.5 29.577 213 144 6 49 258 781 990 1.53e-12 69.7
Msa0321750 AT1G27180.8 29.577 213 144 6 49 258 781 990 1.53e-12 69.7
Msa0321750 AT1G27180.7 29.577 213 144 6 49 258 607 816 1.98e-12 69.3
Msa0321750 AT1G27180.6 29.577 213 144 6 49 258 607 816 1.98e-12 69.3
Msa0321750 AT1G27180.4 29.577 213 144 6 49 258 607 816 1.98e-12 69.3
Msa0321750 AT3G24240.1 35.484 217 130 10 56 265 289 502 2.65e-12 68.6
Msa0321750 AT4G04220.1 33.333 243 140 12 37 263 74 310 3.18e-12 68.2
Msa0321750 AT5G46330.2 34.222 225 115 10 74 268 178 399 4.77e-12 67.8
Msa0321750 AT5G46330.1 34.222 225 115 10 74 268 178 399 4.77e-12 67.8
Msa0321750 AT5G07180.1 33.803 213 132 9 67 273 75 284 5.67e-12 67.8
Msa0321750 AT4G13920.1 30.542 203 130 9 65 258 79 279 1.08e-11 66.6
Msa0321750 AT3G11010.3 32.189 233 145 10 39 265 124 349 1.35e-11 66.6
Msa0321750 AT3G11010.5 32.189 233 145 10 39 265 61 286 1.40e-11 66.2
Msa0321750 AT5G17680.2 26.587 252 145 6 45 260 762 1009 2.86e-11 65.5
Msa0321750 AT5G17680.1 26.587 252 145 6 45 260 761 1008 2.94e-11 65.5
Msa0321750 AT5G61480.1 31.556 225 143 10 51 267 114 335 3.46e-11 65.1
Msa0321750 AT3G47570.1 32.927 246 154 11 91 328 338 580 3.50e-11 65.1
Msa0321750 AT2G24130.1 34.247 219 134 10 57 267 337 553 4.37e-11 65.1
Msa0321750 AT2G24130.2 34.247 219 134 10 57 267 352 568 4.67e-11 64.7
Msa0321750 AT5G11250.3 34.328 268 158 16 50 307 603 862 4.76e-11 64.7
Msa0321750 AT5G11250.1 34.328 268 158 16 50 307 603 862 4.76e-11 64.7
Msa0321750 AT1G75640.1 29.126 206 136 9 70 267 293 496 5.63e-11 64.7
Msa0321750 AT5G11250.2 34.328 268 158 16 50 307 685 944 5.63e-11 64.7
Msa0321750 AT4G20140.1 30.435 230 125 11 70 267 580 806 8.13e-11 64.3
Msa0321750 AT5G27060.3 30.622 209 117 7 63 267 119 303 9.69e-11 63.9
Msa0321750 AT5G27060.1 30.622 209 117 7 63 267 119 303 9.69e-11 63.9
Msa0321750 AT5G27060.2 30.622 209 117 7 63 267 120 304 9.87e-11 63.9

Find 111 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAATTCTGACTCACATTT+AGG 0.163400 2_4:+23343519 None:intergenic
CACCATCCACCATCTCTTCT+TGG 0.191377 2_4:+23343259 None:intergenic
TTCCCTCCACGCTTAGCTTT+TGG 0.217550 2_4:+23343381 None:intergenic
GAGTCAGAATTTCCGGTATT+TGG 0.246732 2_4:-23343510 Msa0321750:CDS
GTTGAGATTCAGTTCTTCTA+AGG 0.278250 2_4:+23343739 None:intergenic
TAAATGTGAGTCAGAATTTC+CGG 0.296177 2_4:-23343517 Msa0321750:CDS
CAGCAACCATTGAGAATTGC+AGG 0.306044 2_4:-23345470 Msa0321750:intron
AGAGAGACAACCAATGGAAT+TGG 0.315520 2_4:+23345539 None:intergenic
GAAATTCTGACTCACATTTA+GGG 0.316541 2_4:+23343520 None:intergenic
CAATGGTTGCTGGGAGGGAT+TGG 0.332249 2_4:+23345481 None:intergenic
TCTTGCTTCACCAGGCTTCA+TGG 0.336493 2_4:-23343147 Msa0321750:CDS
CTTGCTTCACCAGGCTTCAT+GGG 0.358409 2_4:-23343146 Msa0321750:CDS
TCCACTGGAGGTGGTGGAGC+AGG 0.362764 2_4:-23343342 Msa0321750:CDS
GCTAAATCAAGAGAAAGTTT+TGG 0.364003 2_4:+23346346 None:intergenic
CTGCAATTCTCAATGGTTGC+TGG 0.367867 2_4:+23345471 None:intergenic
CCCGCTCAGTTCGCCTCCAC+TGG 0.372267 2_4:-23343357 Msa0321750:CDS
CTCAAAGCTTTGAATGTCTC+TGG 0.381783 2_4:-23345513 Msa0321750:CDS
TCTTCTTGGTTGGGATATGA+TGG 0.383328 2_4:+23343273 None:intergenic
CTCTCACATGATGACTCTTA+TGG 0.393115 2_4:-23343612 Msa0321750:CDS
ATCCACCATCTCTTCTTGGT+TGG 0.393964 2_4:+23343263 None:intergenic
ATTATTAAACTTGGAGATGT+TGG 0.403539 2_4:-23345592 Msa0321750:CDS
GGACTTGCATGGCATGTCCT+TGG 0.409480 2_4:-23346377 Msa0321750:CDS
ACTTATCATGTCTCCATTGA+AGG 0.410083 2_4:+23343226 None:intergenic
TGGTGAAGCAAGACCATTCA+TGG 0.416329 2_4:+23343157 None:intergenic
TTCGCCTCCACTGGAGGTGG+TGG 0.417968 2_4:-23343348 Msa0321750:CDS
TCCACCATCTCTTCTTGGTT+GGG 0.419645 2_4:+23343264 None:intergenic
CAACAATATCAAAACATTGC+CGG 0.423660 2_4:-23343429 Msa0321750:CDS
ACGAGGTTCTCAAGGTCGTC+GGG 0.432286 2_4:+23343554 None:intergenic
AGCTCAAAGCCTATTGTGTC+TGG 0.435730 2_4:+23343695 None:intergenic
TGGGAAACGATCAGAACATA+AGG 0.438511 2_4:-23343204 Msa0321750:CDS
GAGAGACAACCAATGGAATT+GGG 0.443595 2_4:+23345540 None:intergenic
CTTCACCAGGCTTCATGGGG+AGG 0.443666 2_4:-23343142 Msa0321750:CDS
TTCAATGGAGACATGATAAG+TGG 0.444496 2_4:-23343224 Msa0321750:CDS
AGCAAACTTCCAGACACAAT+AGG 0.451995 2_4:-23343704 Msa0321750:CDS
ATTCTCAATGGTTGCTGGGA+GGG 0.461311 2_4:+23345476 None:intergenic
ATTGTTAGACAAATTGAGCT+TGG 0.462966 2_4:+23346315 None:intergenic
AACGGCAAGATTATTAAACT+TGG 0.472327 2_4:-23345601 Msa0321750:CDS
CCACTGGAGGTGGTGGAGCA+GGG 0.480559 2_4:-23343341 Msa0321750:CDS
AGGAAGACACACAAGTTTGT+TGG 0.489389 2_4:+23343643 None:intergenic
GACAACCTCCCCATGAAGCC+TGG 0.490735 2_4:+23343137 None:intergenic
TGCAATTCTCAATGGTTGCT+GGG 0.491491 2_4:+23345472 None:intergenic
TGGAGGCATCCGATGGAGTC+CGG 0.491902 2_4:+23343410 None:intergenic
CCAGTGGAGGCGAACTGAGC+GGG 0.492506 2_4:+23343357 None:intergenic
GAACATACCGGAGTCATTAA+CGG 0.493750 2_4:-23345619 Msa0321750:CDS
GTGAAATGTGGGACCTTCAA+TGG 0.499860 2_4:-23343239 Msa0321750:CDS
CAGTTCGCCTCCACTGGAGG+TGG 0.507058 2_4:-23343351 Msa0321750:CDS
GAGTCATTGCCCTACTCCAT+AGG 0.508724 2_4:-23343488 Msa0321750:CDS
AATAAACCTGCAATTCTCAA+TGG 0.511036 2_4:+23345464 None:intergenic
TCAACTTAAGGAATTACGAC+GGG 0.513441 2_4:+23343094 None:intergenic
CAAGATTGACGAGGTTCTCA+AGG 0.516518 2_4:+23343546 None:intergenic
CAAGAGGAGTCCTATGGAGT+AGG 0.516632 2_4:+23343478 None:intergenic
TCATCAGATGACAATGAAGA+TGG 0.519446 2_4:-23346479 Msa0321750:CDS
CTTCATTATCAACCCATGAA+TGG 0.521629 2_4:-23343170 Msa0321750:CDS
ATGAATGGTCTTGCTTCACC+AGG 0.524055 2_4:-23343155 Msa0321750:CDS
TGCTCCACCACCTCCAGTGG+AGG 0.525602 2_4:+23343344 None:intergenic
GAGCTTCTGGAGGCATCCGA+TGG 0.527574 2_4:+23343403 None:intergenic
CTATCTTCCTCCGTCGGCGT+CGG 0.529882 2_4:+23346412 None:intergenic
CCCTGCTCCACCACCTCCAG+TGG 0.531840 2_4:+23343341 None:intergenic
GAAGACGCCGCCGACGCCGA+CGG 0.534937 2_4:-23346422 Msa0321750:CDS
TTTGTCTAACAATAATCTCC+AGG 0.539269 2_4:-23346305 Msa0321750:intron
CTCCAAAAGCTAAGCGTGGA+GGG 0.545563 2_4:-23343383 Msa0321750:CDS
AATTACGACGGGGTGAGAAG+AGG 0.549684 2_4:+23343105 None:intergenic
ACGTACGTTTGAATTGCCTC+AGG 0.550757 2_4:-23343583 Msa0321750:CDS
GACGAGGTTCTCAAGGTCGT+CGG 0.550775 2_4:+23343553 None:intergenic
AGATCCCTACCCAATTCCAT+TGG 0.551883 2_4:-23345549 Msa0321750:CDS
TTGCTTCACCAGGCTTCATG+GGG 0.551908 2_4:-23343145 Msa0321750:CDS
AAGAGGAGTCCTATGGAGTA+GGG 0.556187 2_4:+23343479 None:intergenic
TAGGGACAAGAGGAGTCCTA+TGG 0.559709 2_4:+23343472 None:intergenic
GACAACCAATGGAATTGGGT+AGG 0.561937 2_4:+23345544 None:intergenic
ATCATATGACAACTAGGAAG+AGG 0.567301 2_4:-23346456 Msa0321750:CDS
AGCTAACGTCTAGTTCAACT+AGG 0.568083 2_4:+23343453 None:intergenic
AAAACATTGCCGGACTCCAT+CGG 0.568332 2_4:-23343419 Msa0321750:CDS
GCTCAGTTCGCCTCCACTGG+AGG 0.570036 2_4:-23343354 Msa0321750:CDS
AGATGGTGGATGGTGAAATG+TGG 0.570372 2_4:-23343251 Msa0321750:CDS
ATCATGTGAGAGGTGGAGCT+AGG 0.579817 2_4:+23343623 None:intergenic
ACAACCAATGGAATTGGGTA+GGG 0.580388 2_4:+23345545 None:intergenic
GGGAAACGATCAGAACATAA+GGG 0.581219 2_4:-23343203 Msa0321750:CDS
AGGGCAATGACTCCAAATAC+CGG 0.582603 2_4:+23343498 None:intergenic
GACGCCGCCGACGCCGACGG+AGG 0.587900 2_4:-23346419 Msa0321750:CDS
AATCTTGCCGTTAATGACTC+CGG 0.589085 2_4:+23345612 None:intergenic
GAGTTTAGAGAGACAACCAA+TGG 0.592305 2_4:+23345533 None:intergenic
AGATGGATCATATGACAACT+AGG 0.595001 2_4:-23346462 Msa0321750:CDS
CGGGGTGAGAAGAGGCGAAG+AGG 0.596579 2_4:+23343113 None:intergenic
TCTTCCTCCGTCGGCGTCGG+CGG 0.598751 2_4:+23346415 None:intergenic
TCATATGACAACTAGGAAGA+GGG 0.604678 2_4:-23346455 Msa0321750:CDS
GCTCCAAAAGCTAAGCGTGG+AGG 0.605699 2_4:-23343384 Msa0321750:CDS
ATATCCCAACCAAGAAGAGA+TGG 0.606320 2_4:-23343268 Msa0321750:CDS
AACCAAGAAGAGATGGTGGA+TGG 0.610943 2_4:-23343261 Msa0321750:CDS
GTTCATGTTGTGGACTTGCA+TGG 0.612500 2_4:-23346388 Msa0321750:CDS
CTAGTTCAACTAGGGACAAG+AGG 0.617875 2_4:+23343462 None:intergenic
TCAATGGAGACATGATAAGT+GGG 0.620791 2_4:-23343223 Msa0321750:CDS
AAGAGTCATCATGTGAGAGG+TGG 0.622685 2_4:+23343616 None:intergenic
TCCCAACCAAGAAGAGATGG+TGG 0.632111 2_4:-23343265 Msa0321750:CDS
AATTGGGTAGGGATCTGAGC+TGG 0.634161 2_4:+23345556 None:intergenic
GCTAACGTCTAGTTCAACTA+GGG 0.636021 2_4:+23343454 None:intergenic
CTCAACTTAAGGAATTACGA+CGG 0.637737 2_4:+23343093 None:intergenic
GGGTAGGGATCTGAGCTGGT+TGG 0.642111 2_4:+23345560 None:intergenic
GGTCGTCGGGGAGACTCCTG+AGG 0.642718 2_4:+23343567 None:intergenic
GGTGAAGCAAGACCATTCAT+GGG 0.642951 2_4:+23343158 None:intergenic
CATAAGAGTCATCATGTGAG+AGG 0.643496 2_4:+23343613 None:intergenic
TGAACTCTATCTTCCTCCGT+CGG 0.645634 2_4:+23346406 None:intergenic
GAAGCTCCAAAAGCTAAGCG+TGG 0.653198 2_4:-23343387 Msa0321750:CDS
GATGGTGGATGGTGAAATGT+GGG 0.654566 2_4:-23343250 Msa0321750:CDS
AATTCTCAATGGTTGCTGGG+AGG 0.654863 2_4:+23345475 None:intergenic
GGAAGATAGAGTTCATGTTG+TGG 0.654967 2_4:-23346398 Msa0321750:CDS
TCCAGTGGAGGCGAACTGAG+CGG 0.662649 2_4:+23343356 None:intergenic
GGTGGAGCAGGGATTGCATG+TGG 0.678013 2_4:-23343330 Msa0321750:CDS
CTATATATAACAGAACATAC+CGG 0.683092 2_4:-23345631 Msa0321750:intron
CGAGGTTCTCAAGGTCGTCG+GGG 0.683613 2_4:+23343555 None:intergenic
CAACTTAAGGAATTACGACG+GGG 0.695800 2_4:+23343095 None:intergenic
TTAGGGTTTCAAGATTGACG+AGG 0.703892 2_4:+23343537 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATTAGATGTAATATAA+CGG - chr2_4:23343720-23343739 Msa0321750:CDS 10.0%
!! TGTTATATAAATTATTTGAT+AGG - chr2_4:23343943-23343962 Msa0321750:intron 10.0%
!!! TTTTATTCTTGTTTTTTTTT+AGG - chr2_4:23345409-23345428 Msa0321750:intron 10.0%
!!! TTTATTCTTGTTTTTTTTTA+GGG - chr2_4:23345410-23345429 Msa0321750:intron 10.0%
!!! TTTTTTTTGAAAGAATTTTT+AGG - chr2_4:23345440-23345459 Msa0321750:intron 10.0%
!!! TTTTTTTGAAAGAATTTTTA+GGG - chr2_4:23345441-23345460 Msa0321750:intron 10.0%
!!! AAAGATTATTCTATTTGTTA+TGG + chr2_4:23343552-23343571 None:intergenic 15.0%
!! AATAGTTAATAAGGATAATT+TGG + chr2_4:23344380-23344399 None:intergenic 15.0%
!! AAATTATCCTTATTAACTAT+TGG - chr2_4:23344379-23344398 Msa0321750:intron 15.0%
!!! TAGTTTTAAAAGTAATACTT+TGG - chr2_4:23344403-23344422 Msa0321750:intron 15.0%
!!! CATTTTGAAACAAATTTTTT+TGG - chr2_4:23344506-23344525 Msa0321750:intron 15.0%
!!! AATTTGTTAATATAAGTCTT+TGG + chr2_4:23345110-23345129 None:intergenic 15.0%
!! TATTAACAAATTACATTTGT+AGG - chr2_4:23345118-23345137 Msa0321750:intron 15.0%
!!! TTATTCTTGTTTTTTTTTAG+GGG - chr2_4:23345411-23345430 Msa0321750:intron 15.0%
!!! TTTTTTGAAAGAATTTTTAG+GGG - chr2_4:23345442-23345461 Msa0321750:intron 15.0%
!! TTATTCAATCAATATCTAAT+TGG + chr2_4:23345657-23345676 None:intergenic 15.0%
!!! CCAATTTTATCATATAATAA+TGG + chr2_4:23345722-23345741 None:intergenic 15.0%
!! CCATTATTATATGATAAAAT+TGG - chr2_4:23345719-23345738 Msa0321750:intron 15.0%
!!! AGTACAATGCAATTTTATTA+AGG - chr2_4:23343365-23343384 Msa0321750:CDS 20.0%
!!! TTATTAAGGTTTTGAAGTTT+AGG - chr2_4:23343379-23343398 Msa0321750:CDS 20.0%
!! AATAGAATAATCTTTGTTGA+AGG - chr2_4:23343557-23343576 Msa0321750:CDS 20.0%
!! ATAGAATAATCTTTGTTGAA+GGG - chr2_4:23343558-23343577 Msa0321750:CDS 20.0%
!! AAACTAACCAATAGTTAATA+AGG + chr2_4:23344389-23344408 None:intergenic 20.0%
!! AGAAAAAGTTATTATTGCAT+TGG - chr2_4:23344466-23344485 Msa0321750:intron 20.0%
!!! TTGCATAACTTTTTTTTTAG+TGG - chr2_4:23344713-23344732 Msa0321750:intron 20.0%
!! AAACAAGAATAAAAGTCTAT+CGG + chr2_4:23345403-23345422 None:intergenic 20.0%
!! TGATTAGAATTGAAAATTTG+AGG + chr2_4:23345566-23345585 None:intergenic 20.0%
! TCCAAATCTTAAAATCATAG+TGG - chr2_4:23343517-23343536 Msa0321750:CDS 25.0%
!!! ACCACTATGATTTTAAGATT+TGG + chr2_4:23343521-23343540 None:intergenic 25.0%
!!! GGATTTTTTTGATAAGTATC+TGG - chr2_4:23343606-23343625 Msa0321750:CDS 25.0%
! CTATATATAACAGAACATAC+CGG - chr2_4:23343980-23343999 Msa0321750:intron 25.0%
! ATTATTAAACTTGGAGATGT+TGG - chr2_4:23344019-23344038 Msa0321750:intron 25.0%
!!! TTTAAAATGAGTGTCGTTTT+AGG - chr2_4:23344298-23344317 Msa0321750:intron 25.0%
! TGTGCATAGTATTAATTAGA+GGG - chr2_4:23344432-23344451 Msa0321750:intron 25.0%
!!! ACATGTTTTTAGTTGTGTAT+AGG - chr2_4:23344602-23344621 Msa0321750:intron 25.0%
!!! ATGATTAGATAACTTGTTTG+AGG + chr2_4:23345040-23345059 None:intergenic 25.0%
! TTTATTTGCATGACATCATT+AGG + chr2_4:23345192-23345211 None:intergenic 25.0%
! ACAAATATTGAACCAGAATT+GGG + chr2_4:23345313-23345332 None:intergenic 25.0%
! AACAAATATTGAACCAGAAT+TGG + chr2_4:23345314-23345333 None:intergenic 25.0%
! ACTAACCATGATTAAAAATC+CGG - chr2_4:23345376-23345395 Msa0321750:intron 25.0%
! AAAAACGAGAAATTCATGAT+GGG + chr2_4:23345510-23345529 None:intergenic 25.0%
! TAAAAACGAGAAATTCATGA+TGG + chr2_4:23345511-23345530 None:intergenic 25.0%
! TCAATCAATATCTAATTGGT+AGG + chr2_4:23345653-23345672 None:intergenic 25.0%
! GCTAAATCAAGAGAAAGTTT+TGG + chr2_4:23343268-23343287 None:intergenic 30.0%
ATTGTTAGACAAATTGAGCT+TGG + chr2_4:23343299-23343318 None:intergenic 30.0%
TTTGTCTAACAATAATCTCC+AGG - chr2_4:23343306-23343325 Msa0321750:CDS 30.0%
AATTGTGAATTTGTCAACCT+TGG + chr2_4:23343340-23343359 None:intergenic 30.0%
! TGGATCTAAATCCATGATTT+TGG + chr2_4:23343501-23343520 None:intergenic 30.0%
AATCTTTGTTGAAGGGAAAA+TGG - chr2_4:23343565-23343584 Msa0321750:CDS 30.0%
AACGGCAAGATTATTAAACT+TGG - chr2_4:23344010-23344029 Msa0321750:intron 30.0%
AATAAACCTGCAATTCTCAA+TGG + chr2_4:23344150-23344169 None:intergenic 30.0%
! ACCCATATCAAGCTAATTTT+AGG - chr2_4:23344254-23344273 Msa0321750:intron 30.0%
! CCCTAAAATTAGCTTGATAT+GGG + chr2_4:23344258-23344277 None:intergenic 30.0%
! CCCATATCAAGCTAATTTTA+GGG - chr2_4:23344255-23344274 Msa0321750:intron 30.0%
! TCCCTAAAATTAGCTTGATA+TGG + chr2_4:23344259-23344278 None:intergenic 30.0%
!!! GACACTCATTTTAAAACTGA+GGG + chr2_4:23344293-23344312 None:intergenic 30.0%
GTGTGCATAGTATTAATTAG+AGG - chr2_4:23344431-23344450 Msa0321750:intron 30.0%
!! AAGCAACACTCATTTTGAAA+CGG - chr2_4:23344532-23344551 Msa0321750:intron 30.0%
GCAAAACACAATAATGAACA+TGG + chr2_4:23344697-23344716 None:intergenic 30.0%
!!! AACTTTTTTTTTAGTGGTCG+TGG - chr2_4:23344719-23344738 Msa0321750:intron 30.0%
!! ATATAAGTCTTTGGTTTGCA+TGG + chr2_4:23345101-23345120 None:intergenic 30.0%
AAAACGAGAAATTCATGATG+GGG + chr2_4:23345509-23345528 None:intergenic 30.0%
!!! CGATATTGATTTTGTGATGT+TGG + chr2_4:23345602-23345621 None:intergenic 30.0%
GAAATTCTGACTCACATTTA+GGG + chr2_4:23346094-23346113 None:intergenic 30.0%
TAAATGTGAGTCAGAATTTC+CGG - chr2_4:23346094-23346113 Msa0321750:intron 30.0%
CAACAATATCAAAACATTGC+CGG - chr2_4:23346182-23346201 Msa0321750:intron 30.0%
TCATCAGATGACAATGAAGA+TGG - chr2_4:23343132-23343151 Msa0321750:CDS 35.0%
AGATGGATCATATGACAACT+AGG - chr2_4:23343149-23343168 Msa0321750:CDS 35.0%
ATCATATGACAACTAGGAAG+AGG - chr2_4:23343155-23343174 Msa0321750:CDS 35.0%
TCATATGACAACTAGGAAGA+GGG - chr2_4:23343156-23343175 Msa0321750:CDS 35.0%
TCATGATGAGATCAATAGCT+AGG - chr2_4:23343456-23343475 Msa0321750:CDS 35.0%
TGGCATAATCTTGTACTACT+TGG - chr2_4:23343585-23343604 Msa0321750:CDS 35.0%
!!! CGACACTCATTTTAAAACTG+AGG + chr2_4:23344294-23344313 None:intergenic 35.0%
!!! AACACTCATTTTGAAACGGA+GGG - chr2_4:23344536-23344555 Msa0321750:intron 35.0%
TGCATAACTATCTACCAAGA+CGG - chr2_4:23344782-23344801 Msa0321750:intron 35.0%
!!! GTTTTTTTAAGTTCCCGTCT+TGG + chr2_4:23344799-23344818 None:intergenic 35.0%
AATATTGAACCAGAATTGGG+AGG + chr2_4:23345310-23345329 None:intergenic 35.0%
AGAATAAAAGTCTATCGGAC+CGG + chr2_4:23345398-23345417 None:intergenic 35.0%
AAACGAGAAATTCATGATGG+GGG + chr2_4:23345508-23345527 None:intergenic 35.0%
GTGCAATTGTGCTAAAAACT+TGG - chr2_4:23345743-23345762 Msa0321750:intron 35.0%
GTTGAGATTCAGTTCTTCTA+AGG + chr2_4:23345875-23345894 None:intergenic 35.0%
GGAAATTCTGACTCACATTT+AGG + chr2_4:23346095-23346114 None:intergenic 35.0%
ACTTATCATGTCTCCATTGA+AGG + chr2_4:23346388-23346407 None:intergenic 35.0%
TTCAATGGAGACATGATAAG+TGG - chr2_4:23346387-23346406 Msa0321750:CDS 35.0%
TCAATGGAGACATGATAAGT+GGG - chr2_4:23346388-23346407 Msa0321750:CDS 35.0%
CTTCATTATCAACCCATGAA+TGG - chr2_4:23346441-23346460 Msa0321750:CDS 35.0%
TCAACTTAAGGAATTACGAC+GGG + chr2_4:23346520-23346539 None:intergenic 35.0%
GGAAGATAGAGTTCATGTTG+TGG - chr2_4:23343213-23343232 Msa0321750:CDS 40.0%
!!! AAGTTTTGGAAGTGAGTCCA+AGG + chr2_4:23343254-23343273 None:intergenic 40.0%
ATCTCCAGGTATAATCTCCA+AGG - chr2_4:23343320-23343339 Msa0321750:CDS 40.0%
TCAACCTTGGAGATTATACC+TGG + chr2_4:23343327-23343346 None:intergenic 40.0%
TGAGATCAATAGCTAGGAAC+AGG - chr2_4:23343462-23343481 Msa0321750:CDS 40.0%
GAACATACCGGAGTCATTAA+CGG - chr2_4:23343992-23344011 Msa0321750:intron 40.0%
AATCTTGCCGTTAATGACTC+CGG + chr2_4:23344002-23344021 None:intergenic 40.0%
ACAACCAATGGAATTGGGTA+GGG + chr2_4:23344069-23344088 None:intergenic 40.0%
GAGAGACAACCAATGGAATT+GGG + chr2_4:23344074-23344093 None:intergenic 40.0%
AGAGAGACAACCAATGGAAT+TGG + chr2_4:23344075-23344094 None:intergenic 40.0%
GAGTTTAGAGAGACAACCAA+TGG + chr2_4:23344081-23344100 None:intergenic 40.0%
!! CTCAAAGCTTTGAATGTCTC+TGG - chr2_4:23344098-23344117 Msa0321750:intron 40.0%
TGCAATTCTCAATGGTTGCT+GGG + chr2_4:23344142-23344161 None:intergenic 40.0%
!!! CAACACTCATTTTGAAACGG+AGG - chr2_4:23344535-23344554 Msa0321750:intron 40.0%
TCTTGGTATAAGTCTTGGTC+GGG + chr2_4:23344752-23344771 None:intergenic 40.0%
CTCTTGGTATAAGTCTTGGT+CGG + chr2_4:23344753-23344772 None:intergenic 40.0%
GACCAAGACTTATACCAAGA+GGG - chr2_4:23344752-23344771 Msa0321750:intron 40.0%
ACCAAGACTTATACCAAGAG+GGG - chr2_4:23344753-23344772 Msa0321750:intron 40.0%
GCATAACTATCTACCAAGAC+GGG - chr2_4:23344783-23344802 Msa0321750:intron 40.0%
!! CTTTGGTTTGCATGGTACAT+GGG + chr2_4:23345093-23345112 None:intergenic 40.0%
!! TCTTTGGTTTGCATGGTACA+TGG + chr2_4:23345094-23345113 None:intergenic 40.0%
!! AATTGGGAGGTTTTGTCTGT+TGG + chr2_4:23345297-23345316 None:intergenic 40.0%
! TCGGACCGGATTTTTAATCA+TGG + chr2_4:23345384-23345403 None:intergenic 40.0%
GCATGCTTCACGTCTTTATA+AGG - chr2_4:23345681-23345700 Msa0321750:intron 40.0%
!! ATTCAGTTCTTCTAAGGCTC+TGG + chr2_4:23345869-23345888 None:intergenic 40.0%
AGCAAACTTCCAGACACAAT+AGG - chr2_4:23345907-23345926 Msa0321750:intron 40.0%
AGGAAGACACACAAGTTTGT+TGG + chr2_4:23345971-23345990 None:intergenic 40.0%
CATAAGAGTCATCATGTGAG+AGG + chr2_4:23346001-23346020 None:intergenic 40.0%
CTCTCACATGATGACTCTTA+TGG - chr2_4:23345999-23346018 Msa0321750:intron 40.0%
TTAGGGTTTCAAGATTGACG+AGG + chr2_4:23346077-23346096 None:intergenic 40.0%
GAGTCAGAATTTCCGGTATT+TGG - chr2_4:23346101-23346120 Msa0321750:intron 40.0%
! GCTAACGTCTAGTTCAACTA+GGG + chr2_4:23346160-23346179 None:intergenic 40.0%
! AGCTAACGTCTAGTTCAACT+AGG + chr2_4:23346161-23346180 None:intergenic 40.0%
TCTTCTTGGTTGGGATATGA+TGG + chr2_4:23346341-23346360 None:intergenic 40.0%
ATATCCCAACCAAGAAGAGA+TGG - chr2_4:23346343-23346362 Msa0321750:CDS 40.0%
TGGGAAACGATCAGAACATA+AGG - chr2_4:23346407-23346426 Msa0321750:CDS 40.0%
GGGAAACGATCAGAACATAA+GGG - chr2_4:23346408-23346427 Msa0321750:CDS 40.0%
CAACTTAAGGAATTACGACG+GGG + chr2_4:23346519-23346538 None:intergenic 40.0%
TGAACTCTATCTTCCTCCGT+CGG + chr2_4:23343208-23343227 None:intergenic 45.0%
GTTCATGTTGTGGACTTGCA+TGG - chr2_4:23343223-23343242 Msa0321750:CDS 45.0%
GCACTAGAGAGCCAAAATCA+TGG - chr2_4:23343487-23343506 Msa0321750:CDS 45.0%
AGATCCCTACCCAATTCCAT+TGG - chr2_4:23344062-23344081 Msa0321750:intron 45.0%
GACAACCAATGGAATTGGGT+AGG + chr2_4:23344070-23344089 None:intergenic 45.0%
ATTCTCAATGGTTGCTGGGA+GGG + chr2_4:23344138-23344157 None:intergenic 45.0%
AATTCTCAATGGTTGCTGGG+AGG + chr2_4:23344139-23344158 None:intergenic 45.0%
CTGCAATTCTCAATGGTTGC+TGG + chr2_4:23344143-23344162 None:intergenic 45.0%
CAGCAACCATTGAGAATTGC+AGG - chr2_4:23344141-23344160 Msa0321750:intron 45.0%
CTTGGTATAAGTCTTGGTCG+GGG + chr2_4:23344751-23344770 None:intergenic 45.0%
CGACCAAGACTTATACCAAG+AGG - chr2_4:23344751-23344770 Msa0321750:intron 45.0%
TCCCCTCTTGGTATAAGTCT+TGG + chr2_4:23344757-23344776 None:intergenic 45.0%
TTATGCAAAACGTCCCCTCT+TGG + chr2_4:23344769-23344788 None:intergenic 45.0%
CAGACAAAACCTCCCAATTC+TGG - chr2_4:23345298-23345317 Msa0321750:intron 45.0%
AGCTCAAAGCCTATTGTGTC+TGG + chr2_4:23345919-23345938 None:intergenic 45.0%
AAGAGTCATCATGTGAGAGG+TGG + chr2_4:23345998-23346017 None:intergenic 45.0%
ACGTACGTTTGAATTGCCTC+AGG - chr2_4:23346028-23346047 Msa0321750:intron 45.0%
CAAGATTGACGAGGTTCTCA+AGG + chr2_4:23346068-23346087 None:intergenic 45.0%
AGGGCAATGACTCCAAATAC+CGG + chr2_4:23346116-23346135 None:intergenic 45.0%
AAGAGGAGTCCTATGGAGTA+GGG + chr2_4:23346135-23346154 None:intergenic 45.0%
CTAGTTCAACTAGGGACAAG+AGG + chr2_4:23346152-23346171 None:intergenic 45.0%
AAAACATTGCCGGACTCCAT+CGG - chr2_4:23346192-23346211 Msa0321750:intron 45.0%
!!! TTAGCTTTTGGAGCTTCTGG+AGG + chr2_4:23346221-23346240 None:intergenic 45.0%
TCCACCATCTCTTCTTGGTT+GGG + chr2_4:23346350-23346369 None:intergenic 45.0%
ATCCACCATCTCTTCTTGGT+TGG + chr2_4:23346351-23346370 None:intergenic 45.0%
!! AACCAAGAAGAGATGGTGGA+TGG - chr2_4:23346350-23346369 Msa0321750:CDS 45.0%
!! AGATGGTGGATGGTGAAATG+TGG - chr2_4:23346360-23346379 Msa0321750:CDS 45.0%
!! GATGGTGGATGGTGAAATGT+GGG - chr2_4:23346361-23346380 Msa0321750:CDS 45.0%
GTGAAATGTGGGACCTTCAA+TGG - chr2_4:23346372-23346391 Msa0321750:CDS 45.0%
GGTGAAGCAAGACCATTCAT+GGG + chr2_4:23346456-23346475 None:intergenic 45.0%
TGGTGAAGCAAGACCATTCA+TGG + chr2_4:23346457-23346476 None:intergenic 45.0%
! ATGAATGGTCTTGCTTCACC+AGG - chr2_4:23346456-23346475 Msa0321750:CDS 45.0%
!!! TTTATTAATATTTTAAAAAC+TGG - chr2_4:23345262-23345281 Msa0321750:intron 5.0%
AATTGGGTAGGGATCTGAGC+TGG + chr2_4:23344058-23344077 None:intergenic 50.0%
!! CAGTTCTTCTAAGGCTCTGG+TGG + chr2_4:23345866-23345885 None:intergenic 50.0%
ATCATGTGAGAGGTGGAGCT+AGG + chr2_4:23345991-23346010 None:intergenic 50.0%
GAGTCATTGCCCTACTCCAT+AGG - chr2_4:23346123-23346142 Msa0321750:intron 50.0%
CAAGAGGAGTCCTATGGAGT+AGG + chr2_4:23346136-23346155 None:intergenic 50.0%
TAGGGACAAGAGGAGTCCTA+TGG + chr2_4:23346142-23346161 None:intergenic 50.0%
!!! CGCTTAGCTTTTGGAGCTTC+TGG + chr2_4:23346224-23346243 None:intergenic 50.0%
GAAGCTCCAAAAGCTAAGCG+TGG - chr2_4:23346224-23346243 Msa0321750:intron 50.0%
CTCCAAAAGCTAAGCGTGGA+GGG - chr2_4:23346228-23346247 Msa0321750:intron 50.0%
!! TTCCCTCCACGCTTAGCTTT+TGG + chr2_4:23346233-23346252 None:intergenic 50.0%
TCCCAACCAAGAAGAGATGG+TGG - chr2_4:23346346-23346365 Msa0321750:CDS 50.0%
CACCATCCACCATCTCTTCT+TGG + chr2_4:23346355-23346374 None:intergenic 50.0%
TCTTGCTTCACCAGGCTTCA+TGG - chr2_4:23346464-23346483 Msa0321750:CDS 50.0%
CTTGCTTCACCAGGCTTCAT+GGG - chr2_4:23346465-23346484 Msa0321750:CDS 50.0%
TTGCTTCACCAGGCTTCATG+GGG - chr2_4:23346466-23346485 Msa0321750:CDS 50.0%
AATTACGACGGGGTGAGAAG+AGG + chr2_4:23346509-23346528 None:intergenic 50.0%
GGACTTGCATGGCATGTCCT+TGG - chr2_4:23343234-23343253 Msa0321750:CDS 55.0%
CAATGGTTGCTGGGAGGGAT+TGG + chr2_4:23344133-23344152 None:intergenic 55.0%
ACGAGGTTCTCAAGGTCGTC+GGG + chr2_4:23346060-23346079 None:intergenic 55.0%
GACGAGGTTCTCAAGGTCGT+CGG + chr2_4:23346061-23346080 None:intergenic 55.0%
GCTCCAAAAGCTAAGCGTGG+AGG - chr2_4:23346227-23346246 Msa0321750:intron 55.0%
CTATCTTCCTCCGTCGGCGT+CGG + chr2_4:23343202-23343221 None:intergenic 60.0%
GGGTAGGGATCTGAGCTGGT+TGG + chr2_4:23344054-23344073 None:intergenic 60.0%
CGAGGTTCTCAAGGTCGTCG+GGG + chr2_4:23346059-23346078 None:intergenic 60.0%
!! TGGAGGCATCCGATGGAGTC+CGG + chr2_4:23346204-23346223 None:intergenic 60.0%
!! GAGCTTCTGGAGGCATCCGA+TGG + chr2_4:23346211-23346230 None:intergenic 60.0%
TCCAGTGGAGGCGAACTGAG+CGG + chr2_4:23346258-23346277 None:intergenic 60.0%
GGTGGAGCAGGGATTGCATG+TGG - chr2_4:23346281-23346300 Msa0321750:intron 60.0%
CTTCACCAGGCTTCATGGGG+AGG - chr2_4:23346469-23346488 Msa0321750:CDS 60.0%
GACAACCTCCCCATGAAGCC+TGG + chr2_4:23346477-23346496 None:intergenic 60.0%
CCAGTGGAGGCGAACTGAGC+GGG + chr2_4:23346257-23346276 None:intergenic 65.0%
GCTCAGTTCGCCTCCACTGG+AGG - chr2_4:23346257-23346276 Msa0321750:intron 65.0%
CAGTTCGCCTCCACTGGAGG+TGG - chr2_4:23346260-23346279 Msa0321750:intron 65.0%
TTCGCCTCCACTGGAGGTGG+TGG - chr2_4:23346263-23346282 Msa0321750:intron 65.0%
TGCTCCACCACCTCCAGTGG+AGG + chr2_4:23346270-23346289 None:intergenic 65.0%
!! TCCACTGGAGGTGGTGGAGC+AGG - chr2_4:23346269-23346288 Msa0321750:intron 65.0%
!! CCACTGGAGGTGGTGGAGCA+GGG - chr2_4:23346270-23346289 Msa0321750:intron 65.0%
CGGGGTGAGAAGAGGCGAAG+AGG + chr2_4:23346501-23346520 None:intergenic 65.0%
! TCTTCCTCCGTCGGCGTCGG+CGG + chr2_4:23343199-23343218 None:intergenic 70.0%
GGTCGTCGGGGAGACTCCTG+AGG + chr2_4:23346047-23346066 None:intergenic 70.0%
CCCGCTCAGTTCGCCTCCAC+TGG - chr2_4:23346254-23346273 Msa0321750:intron 70.0%
CCCTGCTCCACCACCTCCAG+TGG + chr2_4:23346273-23346292 None:intergenic 70.0%
GAAGACGCCGCCGACGCCGA+CGG - chr2_4:23343189-23343208 Msa0321750:CDS 75.0%
! GACGCCGCCGACGCCGACGG+AGG - chr2_4:23343192-23343211 Msa0321750:CDS 85.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 23343094 23346539 23343094 ID=Msa0321750;Name=Msa0321750
chr2_4 mRNA 23343094 23346539 23343094 ID=Msa0321750-mRNA-1;Parent=Msa0321750;Name=Msa0321750-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|3|0|357
chr2_4 exon 23346306 23346539 23346306 ID=Msa0321750-mRNA-1:exon:8548;Parent=Msa0321750-mRNA-1
chr2_4 exon 23345471 23345640 23345471 ID=Msa0321750-mRNA-1:exon:8547;Parent=Msa0321750-mRNA-1
chr2_4 exon 23343094 23343763 23343094 ID=Msa0321750-mRNA-1:exon:8546;Parent=Msa0321750-mRNA-1
chr2_4 CDS 23346306 23346539 23346306 ID=Msa0321750-mRNA-1:cds;Parent=Msa0321750-mRNA-1
chr2_4 CDS 23345471 23345640 23345471 ID=Msa0321750-mRNA-1:cds;Parent=Msa0321750-mRNA-1
chr2_4 CDS 23343094 23343763 23343094 ID=Msa0321750-mRNA-1:cds;Parent=Msa0321750-mRNA-1
Gene Sequence

>Msa0321750

ATGTATGAATTGCAACAATTTCAGTTTCTTCATCAAGATCATCAGATGACAATGAAGATGGATCATATGACAACTAGGAAGAGGGAGACAAAAAAGAAGACGCCGCCGACGCCGACGGAGGAAGATAGAGTTCATGTTGTGGACTTGCATGGCATGTCCTTGGACTCACTTCCAAAACTTTCTCTTGATTTAGCTATTATTTCCAAGCTCAATTTGTCTAACAATAATCTCCAGAACATACCGGAGTCATTAACGGCAAGATTATTAAACTTGGAGATGTTGGACGTGCACTCCAACCAGCTCAGATCCCTACCCAATTCCATTGGTTGTCTCTCTAAACTCAAAGCTTTGAATGTCTCTGGAAACTTCATCCAATCCCTCCCAGCAACCATTGAGAATTGCAGAGCCTTAGAAGAACTGAATCTCAACTTCAACATGTTGAGCAAACTTCCAGACACAATAGGCTTTGAGCTCGTAAAACTCAAGAAGCTTGCAGTTAACTCCAACAAACTTGTGTGTCTTCCTAGCTCCACCTCTCACATGATGACTCTTATGGTTCTCGACGTACGTTTGAATTGCCTCAGGAGTCTCCCCGACGACCTTGAGAACCTCGTCAATCTTGAAACCCTAAATGTGAGTCAGAATTTCCGGTATTTGGAGTCATTGCCCTACTCCATAGGACTCCTCTTGTCCCTAGTTGAACTAGACGTTAGCTACAACAATATCAAAACATTGCCGGACTCCATCGGATGCCTCCAGAAGCTCCAAAAGCTAAGCGTGGAGGGAAACCCGCTCAGTTCGCCTCCACTGGAGGTGGTGGAGCAGGGATTGCATGTGGTGAAAGAGTATATGTGTAACAAAATGAACTCAAGCCATCATATCCCAACCAAGAAGAGATGGTGGATGGTGAAATGTGGGACCTTCAATGGAGACATGATAAGTGGGAAACGATCAGAACATAAGGGTTTCAACAAGCTTCATTATCAACCCATGAATGGTCTTGCTTCACCAGGCTTCATGGGGAGGTTGTCGCCTCTTCGCCTCTTCTCACCCCGTCGTAATTCCTTAAGTTGA
Protein sequence

>Msa0321750

MYELQQFQFLHQDHQMTMKMDHMTTRKRETKKKTPPTPTEEDRVHVVDLHGMSLDSLPKLSLDLAIISKLNLSNNNLQNIPESLTARLLNLEMLDVHSNQLRSLPNSIGCLSKLKALNVSGNFIQSLPATIENCRALEELNLNFNMLSKLPDTIGFELVKLKKLAVNSNKLVCLPSSTSHMMTLMVLDVRLNCLRSLPDDLENLVNLETLNVSQNFRYLESLPYSIGLLLSLVELDVSYNNIKTLPDSIGCLQKLQKLSVEGNPLSSPPLEVVEQGLHVVKEYMCNKMNSSHHIPTKKRWWMVKCGTFNGDMISGKRSEHKGFNKLHYQPMNGLASPGFMGRLSPLRLFSPRRNSLS*