AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1430950


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1430950 MtrunA17_Chr5g0426511 51.351 148 43 7 1 123 1 144 1.43e-23 88.6
Msa1430950 MtrunA17_Chr2g0279541 61.290 124 15 5 1 123 1 92 4.65e-22 83.2
Msa1430950 MtrunA17_Chr4g0049911 46.789 109 40 3 1 100 1 100 1.66e-15 67.8
Msa1430950 MtrunA17_Chr4g0049921 52.885 104 41 3 1 100 1 100 4.19e-15 66.6
Msa1430950 MtrunA17_Chr4g0049951 45.872 109 41 3 1 100 1 100 8.59e-15 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1430950 AT5G09520.1 36.290 124 65 6 2 112 5 127 1.24e-11 58.2

Find 40 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGAAGTGTTGGTGTAGT+TGG 0.341757 tig0026773:-60888 Msa1430950:intergenic
TTTGGCAATGTTGGGATTGT+AGG 0.346130 tig0026773:-60981 Msa1430950:intergenic
ATAGATGGCTGAGTAGGTTT+TGG 0.351476 tig0026773:-60999 Msa1430950:intergenic
GGGATTGTAGTAGGAATGTT+TGG 0.366842 tig0026773:-61077 Msa1430950:intergenic
GGGAATGTTGGCATAGTGAT+TGG 0.393983 tig0026773:-61098 Msa1430950:intergenic
TGAGGCAATGTTGGAATTGT+TGG 0.400561 tig0026773:-60939 Msa1430950:intergenic
TGTGGAATAGTTGGAATTGT+AGG 0.403230 tig0026773:-61035 Msa1430950:intergenic
TGAAGGTGGTGGAGAAAAGA+AGG 0.426869 tig0026773:-61120 Msa1430950:intergenic
AATGTTGGCAATGTTGGTTT+TGG 0.454379 tig0026773:-60906 Msa1430950:intergenic
GGGATAGTACTAGGAATAGA+TGG 0.460146 tig0026773:-61014 Msa1430950:intergenic
TGCTAAAATGAAGGAACTAT+AGG 0.465331 tig0026773:-60793 Msa1430950:intergenic
TGCTATTTCCTTCTCAAACA+TGG 0.466175 tig0026773:+60824 Msa1430950:CDS
AAGAAGATTGCGAGCTGCTA+GGG 0.477055 tig0026773:-60853 Msa1430950:intergenic
GGTGGCAATGTTGGCAATGT+TGG 0.477572 tig0026773:-60912 Msa1430950:intergenic
ACATTTCCTTGAGGCAATGT+TGG 0.483996 tig0026773:-60948 Msa1430950:intergenic
GGAATGTTGGCATAGTGATT+GGG 0.491312 tig0026773:-61097 Msa1430950:intergenic
ATAGTGATTGGGATTGTAGT+AGG 0.509782 tig0026773:-61086 Msa1430950:intergenic
GGAGAAAAGAAGGGGAATGT+TGG 0.509998 tig0026773:-61110 Msa1430950:intergenic
AATGAAAATTGCTAAAATGA+AGG 0.510394 tig0026773:-60802 Msa1430950:intergenic
AGAGTGAACTGTGGAATAGT+TGG 0.512404 tig0026773:-61044 Msa1430950:intergenic
GAAGGTGGTGGAGAAAAGAA+GGG 0.525536 tig0026773:-61119 Msa1430950:intergenic
GAAGAAGATTGCGAGCTGCT+AGG 0.529340 tig0026773:-60854 Msa1430950:intergenic
GTGGAATAGTTGGAATTGTA+GGG 0.531662 tig0026773:-61034 Msa1430950:intergenic
GTTGGAATTGTTGGCAAAGG+TGG 0.537047 tig0026773:-60930 Msa1430950:intergenic
CTAGGAATAGATGGCTGAGT+AGG 0.540861 tig0026773:-61005 Msa1430950:intergenic
GTAGTAGGAATGTTTGGAAG+TGG 0.541956 tig0026773:-61071 Msa1430950:intergenic
GTTGGCAAAGGTGGCAATGT+TGG 0.545513 tig0026773:-60921 Msa1430950:intergenic
GGAATTGTAGGGATAGTACT+AGG 0.546673 tig0026773:-61023 Msa1430950:intergenic
GTTGGGATTGTAGGCAAAGG+TGG 0.549782 tig0026773:-60972 Msa1430950:intergenic
GTAGGAATGTTTGGAAGTGG+TGG 0.557881 tig0026773:-61068 Msa1430950:intergenic
GAAGGAACTATAGGAGGCCA+TGG 0.565486 tig0026773:-60784 Msa1430950:intergenic
TAAAATGAAGGAACTATAGG+AGG 0.579978 tig0026773:-60790 Msa1430950:intergenic
GGTAATGTCCATGTTTGAGA+AGG 0.594515 tig0026773:-60832 Msa1430950:intergenic
TTGACACGAACTTCAAGGAG+TGG 0.601056 tig0026773:-61147 Msa1430950:intergenic
ACAATTCCAACATTGCCTCA+AGG 0.633830 tig0026773:+60942 Msa1430950:CDS
AAGGTGGTGGAGAAAAGAAG+GGG 0.639486 tig0026773:-61118 None:intergenic
AATGTTGGAATTGTTGGCAA+AGG 0.641702 tig0026773:-60933 None:intergenic
AGTGGTGGAAGAGTGAACTG+TGG 0.689745 tig0026773:-61053 None:intergenic
AATGTTGGGATTGTAGGCAA+AGG 0.702377 tig0026773:-60975 None:intergenic
AAAGGTGGAACATTTCCTTG+AGG 0.706888 tig0026773:-60957 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGAAAATTGCTAAAATGA+AGG - tig0026773:60805-60824 Msa1261620:intergenic 20.0%
TAAAATGAAGGAACTATAGG+AGG - tig0026773:60793-60812 Msa1261620:intergenic 30.0%
TGCTAAAATGAAGGAACTAT+AGG - tig0026773:60796-60815 Msa1261620:intergenic 30.0%
!! TGCTATTTCCTTCTCAAACA+TGG + tig0026773:60824-60843 Msa1430950:CDS 35.0%
!!! AATGTTGGTTTTGGAAGTGT+TGG - tig0026773:60900-60919 Msa1430950:intergenic 35.0%
!!! AATGTTGGCAATGTTGGTTT+TGG - tig0026773:60909-60928 Msa1430950:intergenic 35.0%
AATGTTGGAATTGTTGGCAA+AGG - tig0026773:60936-60955 Msa1430950:intergenic 35.0%
GTGGAATAGTTGGAATTGTA+GGG - tig0026773:61037-61056 Msa1430950:intergenic 35.0%
TGTGGAATAGTTGGAATTGT+AGG - tig0026773:61038-61057 Msa1430950:intergenic 35.0%
ATAGTGATTGGGATTGTAGT+AGG - tig0026773:61089-61108 Msa1430950:intergenic 35.0%
GGTAATGTCCATGTTTGAGA+AGG - tig0026773:60835-60854 Msa1430950:intergenic 40.0%
!! TTTGGAAGTGTTGGTGTAGT+TGG - tig0026773:60891-60910 Msa1430950:intergenic 40.0%
TGAGGCAATGTTGGAATTGT+TGG - tig0026773:60942-60961 Msa1430950:intergenic 40.0%
ACAATTCCAACATTGCCTCA+AGG + tig0026773:60942-60961 Msa1430950:CDS 40.0%
ACATTTCCTTGAGGCAATGT+TGG - tig0026773:60951-60970 Msa1430950:intergenic 40.0%
AAAGGTGGAACATTTCCTTG+AGG - tig0026773:60960-60979 Msa1430950:intergenic 40.0%
AATGTTGGGATTGTAGGCAA+AGG - tig0026773:60978-60997 Msa1430950:intergenic 40.0%
TTTGGCAATGTTGGGATTGT+AGG - tig0026773:60984-61003 Msa1430950:intergenic 40.0%
!! GAGTAGGTTTTGGCAATGTT+GGG - tig0026773:60992-61011 Msa1430950:intergenic 40.0%
!! TGAGTAGGTTTTGGCAATGT+TGG - tig0026773:60993-61012 Msa1430950:intergenic 40.0%
! ATAGATGGCTGAGTAGGTTT+TGG - tig0026773:61002-61021 Msa1430950:intergenic 40.0%
GGGATAGTACTAGGAATAGA+TGG - tig0026773:61017-61036 Msa1430950:intergenic 40.0%
GGAATTGTAGGGATAGTACT+AGG - tig0026773:61026-61045 Msa1430950:intergenic 40.0%
AGAGTGAACTGTGGAATAGT+TGG - tig0026773:61047-61066 Msa1430950:intergenic 40.0%
GTAGTAGGAATGTTTGGAAG+TGG - tig0026773:61074-61093 Msa1430950:intergenic 40.0%
GGGATTGTAGTAGGAATGTT+TGG - tig0026773:61080-61099 Msa1430950:intergenic 40.0%
GGAATGTTGGCATAGTGATT+GGG - tig0026773:61100-61119 Msa1430950:intergenic 40.0%
AAGAAGATTGCGAGCTGCTA+GGG - tig0026773:60856-60875 Msa1430950:intergenic 45.0%
GTTGGAATTGTTGGCAAAGG+TGG - tig0026773:60933-60952 Msa1430950:intergenic 45.0%
CTAGGAATAGATGGCTGAGT+AGG - tig0026773:61008-61027 Msa1430950:intergenic 45.0%
GTAGGAATGTTTGGAAGTGG+TGG - tig0026773:61071-61090 Msa1430950:intergenic 45.0%
GGGAATGTTGGCATAGTGAT+TGG - tig0026773:61101-61120 Msa1430950:intergenic 45.0%
GGAGAAAAGAAGGGGAATGT+TGG - tig0026773:61113-61132 Msa1430950:intergenic 45.0%
!! AAGGTGGTGGAGAAAAGAAG+GGG - tig0026773:61121-61140 Msa1430950:intergenic 45.0%
!! GAAGGTGGTGGAGAAAAGAA+GGG - tig0026773:61122-61141 Msa1430950:intergenic 45.0%
!! TGAAGGTGGTGGAGAAAAGA+AGG - tig0026773:61123-61142 Msa1430950:intergenic 45.0%
!! CAAGGAGTGGTTTTTGAAGG+TGG - tig0026773:61137-61156 Msa1430950:intergenic 45.0%
GAAGAAGATTGCGAGCTGCT+AGG - tig0026773:60857-60876 Msa1430950:intergenic 50.0%
GGTGGCAATGTTGGCAATGT+TGG - tig0026773:60915-60934 Msa1430950:intergenic 50.0%
GTTGGCAAAGGTGGCAATGT+TGG - tig0026773:60924-60943 Msa1430950:intergenic 50.0%
GTTGGGATTGTAGGCAAAGG+TGG - tig0026773:60975-60994 Msa1430950:intergenic 50.0%
! AGTGGTGGAAGAGTGAACTG+TGG - tig0026773:61056-61075 Msa1430950:intergenic 50.0%
!! GGAGTGGTTTTTGAAGGTGG+TGG - tig0026773:61134-61153 Msa1430950:intergenic 50.0%
Chromosome Type Strat End Strand Name
tig0026773 gene 60786 61157 60786 ID=Msa1430950;Name=Msa1430950
tig0026773 mRNA 60786 61157 60786 ID=Msa1430950-mRNA-1;Parent=Msa1430950;Name=Msa1430950-mRNA-1;_AED=0.00;_eAED=0.14;_QI=0|-1|0|1|-1|1|1|0|123
tig0026773 exon 60786 61157 60786 ID=Msa1430950-mRNA-1:exon:7298;Parent=Msa1430950-mRNA-1
tig0026773 CDS 60786 61157 60786 ID=Msa1430950-mRNA-1:cds;Parent=Msa1430950-mRNA-1
Gene Sequence

>Msa1430950

ATGGCCTCCTATAGTTCCTTCATTTTAGCAATTTTCATTGCTATTTCCTTCTCAAACATGGACATTACCCTAGCAGCTCGCAATCTTCTTCAGACAACAAATCCAACTACACCAACACTTCCAAAACCAACATTGCCAACATTGCCACCTTTGCCAACAATTCCAACATTGCCTCAAGGAAATGTTCCACCTTTGCCTACAATCCCAACATTGCCAAAACCTACTCAGCCATCTATTCCTAGTACTATCCCTACAATTCCAACTATTCCACAGTTCACTCTTCCACCACTTCCAAACATTCCTACTACAATCCCAATCACTATGCCAACATTCCCCTTCTTTTCTCCACCACCTTCAAAAACCACTCCTTGA

Protein sequence

>Msa1430950

MASYSSFILAIFIAISFSNMDITLAARNLLQTTNPTTPTLPKPTLPTLPPLPTIPTLPQGNVPPLPTIPTLPKPTQPSIPSTIPTIPTIPQFTLPPLPNIPTTIPITMPTFPFFSPPPSKTTP*