Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0015950 | Msa0016080 | 0.980915 | 2.466267e-151 | -8.615850e-47 |
| Msa0015960 | Msa0016080 | 0.967774 | 9.633875e-128 | -8.615850e-47 |
| Msa0016070 | Msa0016080 | 0.985034 | 2.500596e-162 | -8.615850e-47 |
| Msa0016080 | Msa0024650 | 0.804915 | 1.717882e-49 | -8.615850e-47 |
| Msa0016080 | Msa0028690 | 0.863067 | 3.243698e-64 | -8.615850e-47 |
| Msa0016080 | Msa0047660 | -0.835569 | 1.555164e-56 | -8.615850e-47 |
| Msa0016080 | Msa0060410 | 0.856856 | 2.424174e-62 | -8.615850e-47 |
| Msa0016080 | Msa0088800 | 0.808216 | 3.454031e-50 | -8.615850e-47 |
| Msa0016080 | Msa0111730 | 0.805482 | 1.306823e-49 | -8.615850e-47 |
| Msa0016080 | Msa0119240 | 0.818122 | 2.311241e-52 | -8.615850e-47 |
| Msa0016080 | Msa0136000 | 0.800267 | 1.561318e-48 | -8.615850e-47 |
| Msa0016080 | Msa0163690 | 0.817695 | 2.886185e-52 | -8.615850e-47 |
| Msa0016080 | Msa0168750 | 0.847379 | 1.200222e-59 | -8.615850e-47 |
| Msa0016080 | Msa0180380 | 0.830830 | 2.354714e-55 | -8.615850e-47 |
| Msa0016080 | Msa0189990 | 0.852914 | 3.377218e-61 | -8.615850e-47 |
| Msa0016080 | Msa0193950 | 0.807094 | 5.977538e-50 | -8.615850e-47 |
| Msa0016080 | Msa0210610 | 0.836582 | 8.602011e-57 | -8.615850e-47 |
| Msa0016080 | Msa0217060 | 0.813283 | 2.768534e-51 | -8.615850e-47 |
| Msa0016080 | Msa0218500 | 0.821540 | 3.825156e-53 | -8.615850e-47 |
| Msa0016080 | Msa0219340 | 0.800253 | 1.571729e-48 | -8.615850e-47 |
| Msa0016080 | Msa0224870 | 0.809604 | 1.742827e-50 | -8.615850e-47 |
| Msa0016080 | Msa0245660 | 0.804400 | 2.199646e-49 | -8.615850e-47 |
| Msa0016080 | Msa0258260 | 0.817152 | 3.824550e-52 | -8.615850e-47 |
| Msa0016080 | Msa0286360 | 0.826347 | 2.851355e-54 | -8.615850e-47 |
| Msa0016080 | Msa0289960 | 0.821821 | 3.293482e-53 | -8.615850e-47 |
| Msa0016080 | Msa0297340 | 0.817226 | 3.681481e-52 | -8.615850e-47 |
| Msa0016080 | Msa0297910 | 0.800065 | 1.716575e-48 | -8.615850e-47 |
| Msa0016080 | Msa0307740 | 0.867180 | 1.655072e-65 | -8.615850e-47 |
| Msa0016080 | Msa0343210 | 0.808795 | 2.598457e-50 | -8.615850e-47 |
| Msa0016080 | Msa0356270 | 0.813064 | 3.092555e-51 | -8.615850e-47 |
| Msa0016080 | Msa0383480 | 0.830818 | 2.370003e-55 | -8.615850e-47 |
| Msa0016080 | Msa0415640 | 0.805932 | 1.051440e-49 | -8.615850e-47 |
| Msa0016080 | Msa0418230 | 0.810712 | 1.005806e-50 | -8.615850e-47 |
| Msa0016080 | Msa0433650 | 0.830565 | 2.734723e-55 | -8.615850e-47 |
| Msa0016080 | Msa0434300 | 0.806568 | 7.723004e-50 | -8.615850e-47 |
| Msa0016080 | Msa0464620 | 0.827333 | 1.657506e-54 | -8.615850e-47 |
| Msa0016080 | Msa0477420 | 0.800148 | 1.651232e-48 | -8.615850e-47 |
| Msa0016080 | Msa0490100 | 0.800500 | 1.399877e-48 | -8.615850e-47 |
| Msa0016080 | Msa0521490 | 0.831590 | 1.531377e-55 | -8.615850e-47 |
| Msa0016080 | Msa0521550 | 0.835299 | 1.819110e-56 | -8.615850e-47 |
| Msa0016080 | Msa0522140 | 0.815162 | 1.064789e-51 | -8.615850e-47 |
| Msa0016080 | Msa0547370 | 0.802202 | 6.274540e-49 | -8.615850e-47 |
| Msa0016080 | Msa0588040 | 0.830691 | 2.546754e-55 | -8.615850e-47 |
| Msa0016080 | Msa0590810 | 0.806337 | 8.637597e-50 | -8.615850e-47 |
| Msa0016080 | Msa0602100 | 0.805503 | 1.293825e-49 | -8.615850e-47 |
| Msa0016080 | Msa0628200 | 0.810614 | 1.055902e-50 | -8.615850e-47 |
| Msa0016080 | Msa0637590 | 0.809784 | 1.594660e-50 | -8.615850e-47 |
| Msa0016080 | Msa0643230 | 0.818439 | 1.959233e-52 | -8.615850e-47 |
| Msa0016080 | Msa0644340 | 0.815625 | 8.398202e-52 | -8.615850e-47 |
| Msa0016080 | Msa0658150 | 0.841125 | 5.742237e-58 | -8.615850e-47 |
| Msa0016080 | Msa0661410 | 0.977726 | 2.327020e-144 | -8.615850e-47 |
| Msa0016080 | Msa0661420 | 0.967720 | 1.142911e-127 | -8.615850e-47 |
| Msa0016080 | Msa0682910 | 0.800340 | 1.509126e-48 | -8.615850e-47 |
| Msa0016080 | Msa0691320 | -0.819818 | 9.509952e-53 | -8.615850e-47 |
| Msa0016080 | Msa0703000 | 0.843120 | 1.703364e-58 | -8.615850e-47 |
| Msa0016080 | Msa0736920 | 0.817359 | 3.436134e-52 | -8.615850e-47 |
| Msa0016080 | Msa0758990 | 0.800302 | 1.536383e-48 | -8.615850e-47 |
| Msa0016080 | Msa0769540 | 0.800868 | 1.177840e-48 | -8.615850e-47 |
| Msa0016080 | Msa0773030 | 0.862569 | 4.619414e-64 | -8.615850e-47 |
| Msa0016080 | Msa0778570 | 0.821956 | 3.064416e-53 | -8.615850e-47 |
| Msa0016080 | Msa0800120 | 0.829112 | 6.174509e-55 | -8.615850e-47 |
| Msa0016080 | Msa0800150 | 0.845610 | 3.647239e-59 | -8.615850e-47 |
| Msa0016080 | Msa0800180 | 0.826293 | 2.938035e-54 | -8.615850e-47 |
| Msa0016080 | Msa0810330 | 0.860179 | 2.473013e-63 | -8.615850e-47 |
| Msa0016080 | Msa0815940 | 0.814163 | 1.771969e-51 | -8.615850e-47 |
| Msa0016080 | Msa0838210 | 0.805709 | 1.171337e-49 | -8.615850e-47 |
| Msa0016080 | Msa0850640 | 0.874702 | 5.492125e-68 | -8.615850e-47 |
| Msa0016080 | Msa0870170 | 0.802009 | 6.875032e-49 | -8.615850e-47 |
| Msa0016080 | Msa0892820 | -0.808337 | 3.254170e-50 | -8.615850e-47 |
| Msa0016080 | Msa0901270 | 0.820334 | 7.245881e-53 | -8.615850e-47 |
| Msa0016080 | Msa0905570 | 0.802160 | 6.399749e-49 | -8.615850e-47 |
| Msa0016080 | Msa0907880 | 0.860616 | 1.824858e-63 | -8.615850e-47 |
| Msa0016080 | Msa0925940 | 0.840882 | 6.650840e-58 | -8.615850e-47 |
| Msa0016080 | Msa0966580 | 0.818589 | 1.811562e-52 | -8.615850e-47 |
| Msa0016080 | Msa0966590 | 0.807842 | 4.147771e-50 | -8.615850e-47 |
| Msa0016080 | Msa1001120 | 0.818279 | 2.129983e-52 | -8.615850e-47 |
| Msa0016080 | Msa1001930 | -0.807457 | 5.008829e-50 | -8.615850e-47 |
| Msa0016080 | Msa1015360 | 0.802997 | 4.301992e-49 | -8.615850e-47 |
| Msa0016080 | Msa1019420 | 0.834207 | 3.424541e-56 | -8.615850e-47 |
| Msa0016080 | Msa1034950 | 0.805392 | 1.364930e-49 | -8.615850e-47 |
| Msa0016080 | Msa1075010 | 0.806105 | 9.665790e-50 | -8.615850e-47 |
| Msa0016080 | Msa1092310 | 0.833384 | 5.499855e-56 | -8.615850e-47 |
| Msa0016080 | Msa1100330 | 0.801987 | 6.947062e-49 | -8.615850e-47 |
| Msa0016080 | Msa1107220 | 0.806935 | 6.460541e-50 | -8.615850e-47 |
| Msa0016080 | Msa1150760 | 0.801816 | 7.533614e-49 | -8.615850e-47 |
| Msa0016080 | Msa1154060 | 0.807543 | 4.801030e-50 | -8.615850e-47 |
| Msa0016080 | Msa1154320 | 0.805108 | 1.565430e-49 | -8.615850e-47 |
| Msa0016080 | Msa1162430 | 0.802050 | 6.743742e-49 | -8.615850e-47 |
| Msa0016080 | Msa1167310 | 0.809731 | 1.636878e-50 | -8.615850e-47 |
| Msa0016080 | Msa1193940 | 0.809455 | 1.876361e-50 | -8.615850e-47 |
| Msa0016080 | Msa1202800 | 0.833024 | 6.761893e-56 | -8.615850e-47 |
| Msa0016080 | Msa1203650 | 0.818863 | 1.570116e-52 | -8.615850e-47 |
| Msa0016080 | Msa1211940 | 0.861612 | 9.079975e-64 | -8.615850e-47 |
| Msa0016080 | Msa1212080 | 0.851885 | 6.628543e-61 | -8.615850e-47 |
| Msa0016080 | Msa1215540 | -0.804284 | 2.325274e-49 | -8.615850e-47 |
| Msa0016080 | Msa1221270 | 0.816828 | 4.520921e-52 | -8.615850e-47 |
| Msa0016080 | Msa1223960 | 0.800253 | 1.571922e-48 | -8.615850e-47 |
| Msa0016080 | Msa1223990 | 0.818768 | 1.649647e-52 | -8.615850e-47 |
| Msa0016080 | Msa1255210 | 0.825913 | 3.615793e-54 | -8.615850e-47 |
| Msa0016080 | Msa1263110 | 0.801635 | 8.203545e-49 | -8.615850e-47 |
| Msa0016080 | Msa1268360 | 0.833236 | 5.989175e-56 | -8.615850e-47 |
| Msa0016080 | Msa1268390 | 0.831154 | 1.959991e-55 | -8.615850e-47 |
| Msa0016080 | Msa1268420 | 0.813566 | 2.399510e-51 | -8.615850e-47 |
| Msa0016080 | Msa1269200 | 0.839516 | 1.512564e-57 | -8.615850e-47 |
| Msa0016080 | Msa1295650 | 0.827868 | 1.233546e-54 | -8.615850e-47 |
| Msa0016080 | Msa1301280 | -0.825040 | 5.821276e-54 | -8.615850e-47 |
| Msa0016080 | Msa1343600 | 0.816215 | 6.202806e-52 | -8.615850e-47 |
| Msa0016080 | Msa1344450 | 0.812242 | 4.677795e-51 | -8.615850e-47 |
| Msa0016080 | Msa1348540 | 0.829871 | 4.039243e-55 | -8.615850e-47 |
| Msa0016080 | Msa1376880 | 0.800496 | 1.402729e-48 | -8.615850e-47 |
| Msa0016080 | Msa1384130 | 0.823207 | 1.568066e-53 | -8.615850e-47 |
| Msa0016080 | Msa1395080 | 0.843801 | 1.120626e-58 | -8.615850e-47 |
| Msa0016080 | Msa1413450 | 0.823912 | 1.072878e-53 | -8.615850e-47 |
| Msa0016080 | Msa1416130 | 0.824646 | 7.212457e-54 | -8.615850e-47 |
| Msa0016080 | Msa1430950 | 0.869121 | 3.925756e-66 | -8.615850e-47 |
| Msa0016080 | Msa1433040 | 0.829774 | 4.264198e-55 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0016080 | MtrunA17_Chr1g0167771 | 96.078 | 102 | 4 | 0 | 1 | 102 | 13 | 114 | 6.29e-67 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTCACTGTGTCTTCTATT+AGG | 0.227341 | 1_1:-26795928 | Msa0016080:CDS |
| AAGACACAGTGAGATGTATT+TGG | 0.235014 | 1_1:+26795936 | None:intergenic |
| TTTGGTGGCCAGTGTTTCTT+AGG | 0.235134 | 1_1:+26795820 | None:intergenic |
| GGGATTGTTTGGTCCACTTT+TGG | 0.317502 | 1_1:+26795757 | None:intergenic |
| GTAGGAGGCCAGTGTTTCTT+TGG | 0.327069 | 1_1:+26795802 | None:intergenic |
| TGGAAGAAGCATCCTCCTTC+AGG | 0.366754 | 1_1:-26795664 | Msa0016080:CDS |
| TCTCACTGTGTCTTCTATTA+GGG | 0.371015 | 1_1:-26795927 | Msa0016080:CDS |
| AGACACAGTGAGATGTATTT+GGG | 0.409412 | 1_1:+26795937 | None:intergenic |
| GAAGGAGGATGCTTCTTCCA+TGG | 0.436291 | 1_1:+26795667 | None:intergenic |
| GGAGGCCAGTGTTTCTTTGG+TGG | 0.468858 | 1_1:+26795805 | None:intergenic |
| CTTGTGACTATCCTCAACTA+TGG | 0.473523 | 1_1:+26795705 | None:intergenic |
| ACAAAATGATCTTCCAAAAG+TGG | 0.476864 | 1_1:-26795770 | Msa0016080:CDS |
| AAGGAGGATGCTTCTTCCAT+GGG | 0.483462 | 1_1:+26795668 | None:intergenic |
| GCTTCTTCCATGGGTGCTTG+TGG | 0.496634 | 1_1:+26795677 | None:intergenic |
| GACTATCCTCAACTATGGTA+TGG | 0.539163 | 1_1:+26795710 | None:intergenic |
| AGCATCACCACAAGCACCCA+TGG | 0.549276 | 1_1:-26795684 | Msa0016080:CDS |
| CCTCTGTGAGTGTTAATGCT+TGG | 0.552898 | 1_1:-26795855 | Msa0016080:CDS |
| ATTAATCAATTTCCTGAAGG+AGG | 0.566620 | 1_1:+26795652 | None:intergenic |
| ATGATTAATCAATTTCCTGA+AGG | 0.570240 | 1_1:+26795649 | None:intergenic |
| ACTGGCCACCAAAGAAACAC+TGG | 0.593745 | 1_1:-26795810 | Msa0016080:CDS |
| TTCTCTAGCAGATCTTTCAG+AGG | 0.609208 | 1_1:-26795884 | Msa0016080:CDS |
| ATAAGCAACCTAAGAAACAC+TGG | 0.614646 | 1_1:-26795828 | Msa0016080:CDS |
| AGAAGACCATACCATAGTTG+AGG | 0.630682 | 1_1:-26795716 | Msa0016080:CDS |
| TGAAAGATCTGCTAGAGAAG+TGG | 0.631719 | 1_1:+26795888 | None:intergenic |
| AAGATCTGCTAGAGAAGTGG+TGG | 0.634386 | 1_1:+26795891 | None:intergenic |
| CCAAGCATTAACACTCACAG+AGG | 0.666199 | 1_1:+26795855 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGATCATTTTGTGTAGTTG+TGG | + | chr1_1:26795823-26795842 | None:intergenic | 30.0% |
| ACAAAATGATCTTCCAAAAG+TGG | - | chr1_1:26795830-26795849 | Msa0016080:CDS | 30.0% | |
| AGACACAGTGAGATGTATTT+GGG | + | chr1_1:26795666-26795685 | None:intergenic | 35.0% | |
| AAGACACAGTGAGATGTATT+TGG | + | chr1_1:26795667-26795686 | None:intergenic | 35.0% | |
| ATCTCACTGTGTCTTCTATT+AGG | - | chr1_1:26795672-26795691 | Msa0016080:CDS | 35.0% | |
| TCTCACTGTGTCTTCTATTA+GGG | - | chr1_1:26795673-26795692 | Msa0016080:CDS | 35.0% | |
| ATAAGCAACCTAAGAAACAC+TGG | - | chr1_1:26795772-26795791 | Msa0016080:CDS | 35.0% | |
| ! | TCATTTTGTGTAGTTGTGGT+AGG | + | chr1_1:26795819-26795838 | None:intergenic | 35.0% |
| !!! | GTTTGTTTTGTGGGATTGTT+TGG | + | chr1_1:26795857-26795876 | None:intergenic | 35.0% |
| !!! | CTTGTGCTTGTTTGTTTTGT+GGG | + | chr1_1:26795866-26795885 | None:intergenic | 35.0% |
| !!! | TCTTGTGCTTGTTTGTTTTG+TGG | + | chr1_1:26795867-26795886 | None:intergenic | 35.0% |
| TGAAAGATCTGCTAGAGAAG+TGG | + | chr1_1:26795715-26795734 | None:intergenic | 40.0% | |
| TTCTCTAGCAGATCTTTCAG+AGG | - | chr1_1:26795716-26795735 | Msa0016080:CDS | 40.0% | |
| ! | TTTTGTGTAGTTGTGGTAGG+AGG | + | chr1_1:26795816-26795835 | None:intergenic | 40.0% |
| AGAAGACCATACCATAGTTG+AGG | - | chr1_1:26795884-26795903 | Msa0016080:CDS | 40.0% | |
| GACTATCCTCAACTATGGTA+TGG | + | chr1_1:26795893-26795912 | None:intergenic | 40.0% | |
| CTTGTGACTATCCTCAACTA+TGG | + | chr1_1:26795898-26795917 | None:intergenic | 40.0% | |
| AAGATCTGCTAGAGAAGTGG+TGG | + | chr1_1:26795712-26795731 | None:intergenic | 45.0% | |
| CCAAGCATTAACACTCACAG+AGG | + | chr1_1:26795748-26795767 | None:intergenic | 45.0% | |
| CCTCTGTGAGTGTTAATGCT+TGG | - | chr1_1:26795745-26795764 | Msa0016080:CDS | 45.0% | |
| ! | TTTGGTGGCCAGTGTTTCTT+AGG | + | chr1_1:26795783-26795802 | None:intergenic | 45.0% |
| ! | GGGATTGTTTGGTCCACTTT+TGG | + | chr1_1:26795846-26795865 | None:intergenic | 45.0% |
| AAGGAGGATGCTTCTTCCAT+GGG | + | chr1_1:26795935-26795954 | None:intergenic | 45.0% | |
| ACTGGCCACCAAAGAAACAC+TGG | - | chr1_1:26795790-26795809 | Msa0016080:CDS | 50.0% | |
| GTAGGAGGCCAGTGTTTCTT+TGG | + | chr1_1:26795801-26795820 | None:intergenic | 50.0% | |
| GAAGGAGGATGCTTCTTCCA+TGG | + | chr1_1:26795936-26795955 | None:intergenic | 50.0% | |
| !! | TGGAAGAAGCATCCTCCTTC+AGG | - | chr1_1:26795936-26795955 | Msa0016080:CDS | 50.0% |
| GGAGGCCAGTGTTTCTTTGG+TGG | + | chr1_1:26795798-26795817 | None:intergenic | 55.0% | |
| AGCATCACCACAAGCACCCA+TGG | - | chr1_1:26795916-26795935 | Msa0016080:CDS | 55.0% | |
| GCTTCTTCCATGGGTGCTTG+TGG | + | chr1_1:26795926-26795945 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 26795657 | 26795965 | 26795657 | ID=Msa0016080;Name=Msa0016080 |
| chr1_1 | mRNA | 26795657 | 26795965 | 26795657 | ID=Msa0016080-mRNA-1;Parent=Msa0016080;Name=Msa0016080-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|102 |
| chr1_1 | exon | 26795657 | 26795965 | 26795657 | ID=Msa0016080-mRNA-1:exon:8998;Parent=Msa0016080-mRNA-1 |
| chr1_1 | CDS | 26795657 | 26795965 | 26795657 | ID=Msa0016080-mRNA-1:cds;Parent=Msa0016080-mRNA-1 |
| Gene Sequence |
| Protein sequence |
