Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0016070 | A0A396JK39 | 96.078 | 102 | 4 | 0 | 1 | 102 | 13 | 114 | 3.27e-63 | 196 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0015950 | Msa0016070 | 0.966427 | 6.607536e-126 | -8.615850e-47 |
| Msa0015960 | Msa0016070 | 0.962341 | 9.224278e-121 | -8.615850e-47 |
| Msa0016070 | Msa0016080 | 0.985034 | 2.500596e-162 | -8.615850e-47 |
| Msa0016070 | Msa0028690 | 0.853049 | 3.089120e-61 | -8.615850e-47 |
| Msa0016070 | Msa0047660 | -0.818785 | 1.635609e-52 | -8.615850e-47 |
| Msa0016070 | Msa0060410 | 0.843090 | 1.735009e-58 | -8.615850e-47 |
| Msa0016070 | Msa0119240 | 0.803218 | 3.870892e-49 | -8.615850e-47 |
| Msa0016070 | Msa0163690 | 0.803272 | 3.772332e-49 | -8.615850e-47 |
| Msa0016070 | Msa0168750 | 0.834858 | 2.349985e-56 | -8.615850e-47 |
| Msa0016070 | Msa0180380 | 0.813669 | 2.276783e-51 | -8.615850e-47 |
| Msa0016070 | Msa0189990 | 0.838774 | 2.354956e-57 | -8.615850e-47 |
| Msa0016070 | Msa0193950 | 0.808225 | 3.437827e-50 | -8.615850e-47 |
| Msa0016070 | Msa0210610 | 0.831203 | 1.907072e-55 | -8.615850e-47 |
| Msa0016070 | Msa0217060 | 0.803078 | 4.138068e-49 | -8.615850e-47 |
| Msa0016070 | Msa0218500 | 0.809422 | 1.906949e-50 | -8.615850e-47 |
| Msa0016070 | Msa0258260 | 0.802013 | 6.862123e-49 | -8.615850e-47 |
| Msa0016070 | Msa0286360 | 0.820836 | 5.556613e-53 | -8.615850e-47 |
| Msa0016070 | Msa0289960 | 0.806694 | 7.263635e-50 | -8.615850e-47 |
| Msa0016070 | Msa0307740 | 0.855780 | 5.015433e-62 | -8.615850e-47 |
| Msa0016070 | Msa0343210 | 0.804916 | 1.716525e-49 | -8.615850e-47 |
| Msa0016070 | Msa0383480 | 0.829328 | 5.475063e-55 | -8.615850e-47 |
| Msa0016070 | Msa0415640 | 0.803799 | 2.933256e-49 | -8.615850e-47 |
| Msa0016070 | Msa0433650 | 0.819150 | 1.350914e-52 | -8.615850e-47 |
| Msa0016070 | Msa0464620 | 0.812056 | 5.136306e-51 | -8.615850e-47 |
| Msa0016070 | Msa0521490 | 0.823119 | 1.644318e-53 | -8.615850e-47 |
| Msa0016070 | Msa0521550 | 0.825677 | 4.114950e-54 | -8.615850e-47 |
| Msa0016070 | Msa0522140 | 0.803379 | 3.584965e-49 | -8.615850e-47 |
| Msa0016070 | Msa0588040 | 0.821089 | 4.860108e-53 | -8.615850e-47 |
| Msa0016070 | Msa0590810 | 0.800585 | 1.345364e-48 | -8.615850e-47 |
| Msa0016070 | Msa0628200 | 0.801009 | 1.102174e-48 | -8.615850e-47 |
| Msa0016070 | Msa0658150 | 0.827703 | 1.351050e-54 | -8.615850e-47 |
| Msa0016070 | Msa0661410 | 0.961000 | 3.382623e-119 | -8.615850e-47 |
| Msa0016070 | Msa0661420 | 0.962104 | 1.756907e-120 | -8.615850e-47 |
| Msa0016070 | Msa0691320 | -0.811891 | 5.579704e-51 | -8.615850e-47 |
| Msa0016070 | Msa0703000 | 0.829629 | 4.624709e-55 | -8.615850e-47 |
| Msa0016070 | Msa0736920 | 0.804415 | 2.183647e-49 | -8.615850e-47 |
| Msa0016070 | Msa0773030 | 0.850864 | 1.289075e-60 | -8.615850e-47 |
| Msa0016070 | Msa0778570 | 0.812032 | 5.198330e-51 | -8.615850e-47 |
| Msa0016070 | Msa0800120 | 0.805288 | 1.434942e-49 | -8.615850e-47 |
| Msa0016070 | Msa0800150 | 0.823061 | 1.696727e-53 | -8.615850e-47 |
| Msa0016070 | Msa0800180 | 0.805523 | 1.280995e-49 | -8.615850e-47 |
| Msa0016070 | Msa0810330 | 0.853907 | 1.751614e-61 | -8.615850e-47 |
| Msa0016070 | Msa0815940 | 0.802336 | 5.887920e-49 | -8.615850e-47 |
| Msa0016070 | Msa0850640 | 0.865598 | 5.258665e-65 | -8.615850e-47 |
| Msa0016070 | Msa0901270 | 0.809906 | 1.501060e-50 | -8.615850e-47 |
| Msa0016070 | Msa0907880 | 0.855307 | 6.887291e-62 | -8.615850e-47 |
| Msa0016070 | Msa0925940 | 0.831002 | 2.136895e-55 | -8.615850e-47 |
| Msa0016070 | Msa0966580 | 0.809001 | 2.347381e-50 | -8.615850e-47 |
| Msa0016070 | Msa0966590 | 0.804298 | 2.310761e-49 | -8.615850e-47 |
| Msa0016070 | Msa1001120 | 0.805766 | 1.139172e-49 | -8.615850e-47 |
| Msa0016070 | Msa1019420 | 0.826452 | 2.692748e-54 | -8.615850e-47 |
| Msa0016070 | Msa1092310 | 0.815785 | 7.736344e-52 | -8.615850e-47 |
| Msa0016070 | Msa1167310 | 0.803150 | 3.999480e-49 | -8.615850e-47 |
| Msa0016070 | Msa1202800 | 0.823651 | 1.234993e-53 | -8.615850e-47 |
| Msa0016070 | Msa1211940 | 0.840415 | 8.816183e-58 | -8.615850e-47 |
| Msa0016070 | Msa1212080 | 0.836007 | 1.203853e-56 | -8.615850e-47 |
| Msa0016070 | Msa1223990 | 0.801068 | 1.071824e-48 | -8.615850e-47 |
| Msa0016070 | Msa1255210 | 0.816907 | 4.341033e-52 | -8.615850e-47 |
| Msa0016070 | Msa1268360 | 0.811349 | 7.319056e-51 | -8.615850e-47 |
| Msa0016070 | Msa1268390 | 0.808642 | 2.801504e-50 | -8.615850e-47 |
| Msa0016070 | Msa1269200 | 0.832617 | 8.533011e-56 | -8.615850e-47 |
| Msa0016070 | Msa1295650 | 0.816833 | 4.509245e-52 | -8.615850e-47 |
| Msa0016070 | Msa1301280 | -0.811059 | 8.459041e-51 | -8.615850e-47 |
| Msa0016070 | Msa1343600 | 0.802380 | 5.766254e-49 | -8.615850e-47 |
| Msa0016070 | Msa1348540 | 0.826703 | 2.345529e-54 | -8.615850e-47 |
| Msa0016070 | Msa1384130 | 0.806997 | 6.268551e-50 | -8.615850e-47 |
| Msa0016070 | Msa1395080 | 0.830093 | 3.564779e-55 | -8.615850e-47 |
| Msa0016070 | Msa1413450 | 0.814525 | 1.473871e-51 | -8.615850e-47 |
| Msa0016070 | Msa1416130 | 0.826587 | 2.499137e-54 | -8.615850e-47 |
| Msa0016070 | Msa1430950 | 0.856041 | 4.205923e-62 | -8.615850e-47 |
| Msa0016070 | Msa1433040 | 0.820725 | 5.892871e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0016070 | MtrunA17_Chr1g0167771 | 96.078 | 102 | 4 | 0 | 1 | 102 | 13 | 114 | 6.29e-67 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTCACTGTGTCTTCTATT+AGG | 0.227341 | 1_1:-26789022 | Msa0016070:CDS |
| AAGACACAGTGAGATGTATT+TGG | 0.235014 | 1_1:+26789030 | None:intergenic |
| TTTGGTGGCCAGTGTTTCTT+AGG | 0.235134 | 1_1:+26788914 | None:intergenic |
| GGGATTGTTTGGTCCACTTT+TGG | 0.317502 | 1_1:+26788851 | None:intergenic |
| GTAGGAGGCCAGTGTTTCTT+TGG | 0.327069 | 1_1:+26788896 | None:intergenic |
| TGGAAGAAGCATCCTCCTTC+AGG | 0.366754 | 1_1:-26788758 | Msa0016070:CDS |
| TCTCACTGTGTCTTCTATTA+GGG | 0.371015 | 1_1:-26789021 | Msa0016070:CDS |
| AGACACAGTGAGATGTATTT+GGG | 0.409412 | 1_1:+26789031 | None:intergenic |
| GAAGGAGGATGCTTCTTCCA+TGG | 0.436291 | 1_1:+26788761 | None:intergenic |
| GGAGGCCAGTGTTTCTTTGG+TGG | 0.468858 | 1_1:+26788899 | None:intergenic |
| CTTGTGACTATCCTCAACTA+TGG | 0.473523 | 1_1:+26788799 | None:intergenic |
| ACAAAATGATCTTCCAAAAG+TGG | 0.476864 | 1_1:-26788864 | Msa0016070:CDS |
| AAGGAGGATGCTTCTTCCAT+GGG | 0.483462 | 1_1:+26788762 | None:intergenic |
| GCTTCTTCCATGGGTGCTTG+TGG | 0.496634 | 1_1:+26788771 | None:intergenic |
| GACTATCCTCAACTATGGTA+TGG | 0.539163 | 1_1:+26788804 | None:intergenic |
| AGCATCACCACAAGCACCCA+TGG | 0.549276 | 1_1:-26788778 | Msa0016070:CDS |
| CCTCTGTGAGTGTTAATGCT+TGG | 0.552898 | 1_1:-26788949 | Msa0016070:CDS |
| ATTAATCAATTTCCTGAAGG+AGG | 0.566620 | 1_1:+26788746 | None:intergenic |
| ATGATTAATCAATTTCCTGA+AGG | 0.570240 | 1_1:+26788743 | None:intergenic |
| ACTGGCCACCAAAGAAACAC+TGG | 0.593745 | 1_1:-26788904 | Msa0016070:CDS |
| TTCTCTAGCAGATCTTTCAG+AGG | 0.609208 | 1_1:-26788978 | Msa0016070:CDS |
| ATAAGCAACCTAAGAAACAC+TGG | 0.614646 | 1_1:-26788922 | Msa0016070:CDS |
| AGAAGACCATACCATAGTTG+AGG | 0.630682 | 1_1:-26788810 | Msa0016070:CDS |
| TGAAAGATCTGCTAGAGAAG+TGG | 0.631719 | 1_1:+26788982 | None:intergenic |
| AAGATCTGCTAGAGAAGTGG+TGG | 0.634386 | 1_1:+26788985 | None:intergenic |
| CCAAGCATTAACACTCACAG+AGG | 0.666199 | 1_1:+26788949 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGATCATTTTGTGTAGTTG+TGG | + | chr1_1:26788917-26788936 | None:intergenic | 30.0% |
| ACAAAATGATCTTCCAAAAG+TGG | - | chr1_1:26788924-26788943 | Msa0016070:CDS | 30.0% | |
| AGACACAGTGAGATGTATTT+GGG | + | chr1_1:26788760-26788779 | None:intergenic | 35.0% | |
| AAGACACAGTGAGATGTATT+TGG | + | chr1_1:26788761-26788780 | None:intergenic | 35.0% | |
| ATCTCACTGTGTCTTCTATT+AGG | - | chr1_1:26788766-26788785 | Msa0016070:CDS | 35.0% | |
| TCTCACTGTGTCTTCTATTA+GGG | - | chr1_1:26788767-26788786 | Msa0016070:CDS | 35.0% | |
| ATAAGCAACCTAAGAAACAC+TGG | - | chr1_1:26788866-26788885 | Msa0016070:CDS | 35.0% | |
| ! | TCATTTTGTGTAGTTGTGGT+AGG | + | chr1_1:26788913-26788932 | None:intergenic | 35.0% |
| !!! | GTTTGTTTTGTGGGATTGTT+TGG | + | chr1_1:26788951-26788970 | None:intergenic | 35.0% |
| !!! | CTTGTGCTTGTTTGTTTTGT+GGG | + | chr1_1:26788960-26788979 | None:intergenic | 35.0% |
| !!! | TCTTGTGCTTGTTTGTTTTG+TGG | + | chr1_1:26788961-26788980 | None:intergenic | 35.0% |
| TGAAAGATCTGCTAGAGAAG+TGG | + | chr1_1:26788809-26788828 | None:intergenic | 40.0% | |
| TTCTCTAGCAGATCTTTCAG+AGG | - | chr1_1:26788810-26788829 | Msa0016070:CDS | 40.0% | |
| ! | TTTTGTGTAGTTGTGGTAGG+AGG | + | chr1_1:26788910-26788929 | None:intergenic | 40.0% |
| AGAAGACCATACCATAGTTG+AGG | - | chr1_1:26788978-26788997 | Msa0016070:CDS | 40.0% | |
| GACTATCCTCAACTATGGTA+TGG | + | chr1_1:26788987-26789006 | None:intergenic | 40.0% | |
| CTTGTGACTATCCTCAACTA+TGG | + | chr1_1:26788992-26789011 | None:intergenic | 40.0% | |
| AAGATCTGCTAGAGAAGTGG+TGG | + | chr1_1:26788806-26788825 | None:intergenic | 45.0% | |
| CCAAGCATTAACACTCACAG+AGG | + | chr1_1:26788842-26788861 | None:intergenic | 45.0% | |
| CCTCTGTGAGTGTTAATGCT+TGG | - | chr1_1:26788839-26788858 | Msa0016070:CDS | 45.0% | |
| ! | TTTGGTGGCCAGTGTTTCTT+AGG | + | chr1_1:26788877-26788896 | None:intergenic | 45.0% |
| ! | GGGATTGTTTGGTCCACTTT+TGG | + | chr1_1:26788940-26788959 | None:intergenic | 45.0% |
| AAGGAGGATGCTTCTTCCAT+GGG | + | chr1_1:26789029-26789048 | None:intergenic | 45.0% | |
| ACTGGCCACCAAAGAAACAC+TGG | - | chr1_1:26788884-26788903 | Msa0016070:CDS | 50.0% | |
| GTAGGAGGCCAGTGTTTCTT+TGG | + | chr1_1:26788895-26788914 | None:intergenic | 50.0% | |
| GAAGGAGGATGCTTCTTCCA+TGG | + | chr1_1:26789030-26789049 | None:intergenic | 50.0% | |
| !! | TGGAAGAAGCATCCTCCTTC+AGG | - | chr1_1:26789030-26789049 | Msa0016070:CDS | 50.0% |
| GGAGGCCAGTGTTTCTTTGG+TGG | + | chr1_1:26788892-26788911 | None:intergenic | 55.0% | |
| AGCATCACCACAAGCACCCA+TGG | - | chr1_1:26789010-26789029 | Msa0016070:CDS | 55.0% | |
| GCTTCTTCCATGGGTGCTTG+TGG | + | chr1_1:26789020-26789039 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 26788751 | 26789059 | 26788751 | ID=Msa0016070;Name=Msa0016070 |
| chr1_1 | mRNA | 26788751 | 26789059 | 26788751 | ID=Msa0016070-mRNA-1;Parent=Msa0016070;Name=Msa0016070-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|102 |
| chr1_1 | exon | 26788751 | 26789059 | 26788751 | ID=Msa0016070-mRNA-1:exon:8997;Parent=Msa0016070-mRNA-1 |
| chr1_1 | CDS | 26788751 | 26789059 | 26788751 | ID=Msa0016070-mRNA-1:cds;Parent=Msa0016070-mRNA-1 |
| Gene Sequence |
| Protein sequence |
