Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0015950 | Msa0015960 | 0.981092 | 9.359728e-152 | -8.615850e-47 |
| Msa0015950 | Msa0016070 | 0.966427 | 6.607536e-126 | -8.615850e-47 |
| Msa0015950 | Msa0016080 | 0.980915 | 2.466267e-151 | -8.615850e-47 |
| Msa0015950 | Msa0028690 | 0.851577 | 8.108173e-61 | -8.615850e-47 |
| Msa0015950 | Msa0047660 | -0.834312 | 3.223565e-56 | -8.615850e-47 |
| Msa0015950 | Msa0060410 | 0.839323 | 1.697459e-57 | -8.615850e-47 |
| Msa0015950 | Msa0119240 | 0.800034 | 1.741154e-48 | -8.615850e-47 |
| Msa0015950 | Msa0168750 | 0.829770 | 4.274454e-55 | -8.615850e-47 |
| Msa0015950 | Msa0180380 | 0.810175 | 1.313688e-50 | -8.615850e-47 |
| Msa0015950 | Msa0189990 | 0.841445 | 4.732522e-58 | -8.615850e-47 |
| Msa0015950 | Msa0193950 | 0.807156 | 5.799105e-50 | -8.615850e-47 |
| Msa0015950 | Msa0210610 | 0.827044 | 1.944421e-54 | -8.615850e-47 |
| Msa0015950 | Msa0217060 | 0.807154 | 5.807036e-50 | -8.615850e-47 |
| Msa0015950 | Msa0218500 | 0.814284 | 1.666327e-51 | -8.615850e-47 |
| Msa0015950 | Msa0258260 | 0.804660 | 1.941606e-49 | -8.615850e-47 |
| Msa0015950 | Msa0286360 | 0.824853 | 6.443659e-54 | -8.615850e-47 |
| Msa0015950 | Msa0289960 | 0.814102 | 1.827343e-51 | -8.615850e-47 |
| Msa0015950 | Msa0297340 | 0.805980 | 1.027162e-49 | -8.615850e-47 |
| Msa0015950 | Msa0307740 | 0.859533 | 3.874169e-63 | -8.615850e-47 |
| Msa0015950 | Msa0383480 | 0.821927 | 3.111365e-53 | -8.615850e-47 |
| Msa0015950 | Msa0415640 | 0.802623 | 5.137986e-49 | -8.615850e-47 |
| Msa0015950 | Msa0433650 | 0.829622 | 4.644510e-55 | -8.615850e-47 |
| Msa0015950 | Msa0464620 | 0.816527 | 5.280941e-52 | -8.615850e-47 |
| Msa0015950 | Msa0477420 | 0.805817 | 1.111217e-49 | -8.615850e-47 |
| Msa0015950 | Msa0521490 | 0.826316 | 2.899765e-54 | -8.615850e-47 |
| Msa0015950 | Msa0521550 | 0.832185 | 1.091662e-55 | -8.615850e-47 |
| Msa0015950 | Msa0522140 | 0.809036 | 2.307487e-50 | -8.615850e-47 |
| Msa0015950 | Msa0588040 | 0.823893 | 1.083718e-53 | -8.615850e-47 |
| Msa0015950 | Msa0590810 | 0.809718 | 1.647855e-50 | -8.615850e-47 |
| Msa0015950 | Msa0637590 | 0.804855 | 1.767532e-49 | -8.615850e-47 |
| Msa0015950 | Msa0643230 | 0.800260 | 1.566604e-48 | -8.615850e-47 |
| Msa0015950 | Msa0658150 | 0.822219 | 2.663324e-53 | -8.615850e-47 |
| Msa0015950 | Msa0661410 | 0.989413 | 5.179607e-178 | -8.615850e-47 |
| Msa0015950 | Msa0661420 | 0.977491 | 6.910818e-144 | -8.615850e-47 |
| Msa0015950 | Msa0691320 | -0.818276 | 2.133463e-52 | -8.615850e-47 |
| Msa0015950 | Msa0703000 | 0.824879 | 6.354353e-54 | -8.615850e-47 |
| Msa0015950 | Msa0736920 | 0.806764 | 7.020835e-50 | -8.615850e-47 |
| Msa0015950 | Msa0773030 | 0.843936 | 1.030800e-58 | -8.615850e-47 |
| Msa0015950 | Msa0778570 | 0.814000 | 1.925093e-51 | -8.615850e-47 |
| Msa0015950 | Msa0800120 | 0.812572 | 3.962931e-51 | -8.615850e-47 |
| Msa0015950 | Msa0800150 | 0.832476 | 9.252000e-56 | -8.615850e-47 |
| Msa0015950 | Msa0800180 | 0.811837 | 5.732097e-51 | -8.615850e-47 |
| Msa0015950 | Msa0810330 | 0.851991 | 6.184553e-61 | -8.615850e-47 |
| Msa0015950 | Msa0815940 | 0.805031 | 1.624191e-49 | -8.615850e-47 |
| Msa0015950 | Msa0850640 | 0.865928 | 4.137692e-65 | -8.615850e-47 |
| Msa0015950 | Msa0901270 | 0.810078 | 1.378386e-50 | -8.615850e-47 |
| Msa0015950 | Msa0907880 | 0.854024 | 1.620643e-61 | -8.615850e-47 |
| Msa0015950 | Msa0925940 | 0.835539 | 1.581914e-56 | -8.615850e-47 |
| Msa0015950 | Msa0966580 | 0.820823 | 5.595705e-53 | -8.615850e-47 |
| Msa0015950 | Msa1001120 | 0.807745 | 4.350904e-50 | -8.615850e-47 |
| Msa0015950 | Msa1019420 | 0.817672 | 2.919865e-52 | -8.615850e-47 |
| Msa0015950 | Msa1092310 | 0.824409 | 8.199227e-54 | -8.615850e-47 |
| Msa0015950 | Msa1162430 | 0.804455 | 2.142677e-49 | -8.615850e-47 |
| Msa0015950 | Msa1193940 | 0.802232 | 6.186034e-49 | -8.615850e-47 |
| Msa0015950 | Msa1202800 | 0.828038 | 1.122620e-54 | -8.615850e-47 |
| Msa0015950 | Msa1211940 | 0.846528 | 2.053067e-59 | -8.615850e-47 |
| Msa0015950 | Msa1212080 | 0.832710 | 8.093537e-56 | -8.615850e-47 |
| Msa0015950 | Msa1223990 | 0.802001 | 6.900872e-49 | -8.615850e-47 |
| Msa0015950 | Msa1255210 | 0.822744 | 2.010829e-53 | -8.615850e-47 |
| Msa0015950 | Msa1268360 | 0.816184 | 6.303280e-52 | -8.615850e-47 |
| Msa0015950 | Msa1268390 | 0.817365 | 3.424211e-52 | -8.615850e-47 |
| Msa0015950 | Msa1268420 | 0.802001 | 6.900781e-49 | -8.615850e-47 |
| Msa0015950 | Msa1269200 | 0.829454 | 5.102243e-55 | -8.615850e-47 |
| Msa0015950 | Msa1295650 | 0.825077 | 5.704281e-54 | -8.615850e-47 |
| Msa0015950 | Msa1301280 | -0.811396 | 7.150545e-51 | -8.615850e-47 |
| Msa0015950 | Msa1343600 | 0.805445 | 1.330256e-49 | -8.615850e-47 |
| Msa0015950 | Msa1344450 | 0.806889 | 6.605449e-50 | -8.615850e-47 |
| Msa0015950 | Msa1348540 | 0.832862 | 7.417337e-56 | -8.615850e-47 |
| Msa0015950 | Msa1384130 | 0.813395 | 2.615586e-51 | -8.615850e-47 |
| Msa0015950 | Msa1395080 | 0.825328 | 4.977519e-54 | -8.615850e-47 |
| Msa0015950 | Msa1413450 | 0.805156 | 1.529291e-49 | -8.615850e-47 |
| Msa0015950 | Msa1416130 | 0.822568 | 2.209538e-53 | -8.615850e-47 |
| Msa0015950 | Msa1430950 | 0.860869 | 1.528369e-63 | -8.615850e-47 |
| Msa0015950 | Msa1433040 | 0.824256 | 8.909671e-54 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0015950 | MtrunA17_Chr1g0167771 | 96.078 | 102 | 4 | 0 | 1 | 102 | 13 | 114 | 6.29e-67 | 196 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTCACTGTGTCTTCTATT+AGG | 0.227341 | 1_1:+26347116 | Msa0015950:CDS |
| AAGACACAGTGAGATGTATT+TGG | 0.235014 | 1_1:-26347108 | None:intergenic |
| TTTGGTGGCCAGTGTTTCTT+AGG | 0.235134 | 1_1:-26347224 | None:intergenic |
| GGGATTGTTTGGTCCACTTT+TGG | 0.317502 | 1_1:-26347287 | None:intergenic |
| GTAGGAGGCCAGTGTTTCTT+TGG | 0.327069 | 1_1:-26347242 | None:intergenic |
| TGGAAGAAGCATCCTCCTTC+AGG | 0.366754 | 1_1:+26347380 | Msa0015950:CDS |
| TCTCACTGTGTCTTCTATTA+GGG | 0.371015 | 1_1:+26347117 | Msa0015950:CDS |
| AGACACAGTGAGATGTATTT+GGG | 0.409412 | 1_1:-26347107 | None:intergenic |
| GAAGGAGGATGCTTCTTCCA+TGG | 0.436291 | 1_1:-26347377 | None:intergenic |
| GGAGGCCAGTGTTTCTTTGG+TGG | 0.468858 | 1_1:-26347239 | None:intergenic |
| CTTGTGACTATCCTCAACTA+TGG | 0.473523 | 1_1:-26347339 | None:intergenic |
| ACAAAATGATCTTCCAAAAG+TGG | 0.476864 | 1_1:+26347274 | Msa0015950:CDS |
| AAGGAGGATGCTTCTTCCAT+GGG | 0.483462 | 1_1:-26347376 | None:intergenic |
| GCTTCTTCCATGGGTGCTTG+TGG | 0.496634 | 1_1:-26347367 | None:intergenic |
| GACTATCCTCAACTATGGTA+TGG | 0.539163 | 1_1:-26347334 | None:intergenic |
| AGCATCACCACAAGCACCCA+TGG | 0.549276 | 1_1:+26347360 | Msa0015950:CDS |
| CCTCTGTGAGTGTTAATGCT+TGG | 0.552898 | 1_1:+26347189 | Msa0015950:CDS |
| ATTAATCAATTTCCTGAAGG+AGG | 0.566620 | 1_1:-26347392 | None:intergenic |
| ATGATTAATCAATTTCCTGA+AGG | 0.570240 | 1_1:-26347395 | None:intergenic |
| ACTGGCCACCAAAGAAACAC+TGG | 0.593745 | 1_1:+26347234 | Msa0015950:CDS |
| TTCTCTAGCAGATCTTTCAG+AGG | 0.609208 | 1_1:+26347160 | Msa0015950:CDS |
| ATAAGCAACCTAAGAAACAC+TGG | 0.614646 | 1_1:+26347216 | Msa0015950:CDS |
| AGAAGACCATACCATAGTTG+AGG | 0.630682 | 1_1:+26347328 | Msa0015950:CDS |
| TGAAAGATCTGCTAGAGAAG+TGG | 0.631719 | 1_1:-26347156 | None:intergenic |
| AAGATCTGCTAGAGAAGTGG+TGG | 0.634386 | 1_1:-26347153 | None:intergenic |
| CCAAGCATTAACACTCACAG+AGG | 0.666199 | 1_1:-26347189 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGATCATTTTGTGTAGTTG+TGG | - | chr1_1:26347267-26347286 | None:intergenic | 30.0% |
| ACAAAATGATCTTCCAAAAG+TGG | + | chr1_1:26347274-26347293 | Msa0015950:CDS | 30.0% | |
| AGACACAGTGAGATGTATTT+GGG | - | chr1_1:26347110-26347129 | None:intergenic | 35.0% | |
| AAGACACAGTGAGATGTATT+TGG | - | chr1_1:26347111-26347130 | None:intergenic | 35.0% | |
| ATCTCACTGTGTCTTCTATT+AGG | + | chr1_1:26347116-26347135 | Msa0015950:CDS | 35.0% | |
| TCTCACTGTGTCTTCTATTA+GGG | + | chr1_1:26347117-26347136 | Msa0015950:CDS | 35.0% | |
| ATAAGCAACCTAAGAAACAC+TGG | + | chr1_1:26347216-26347235 | Msa0015950:CDS | 35.0% | |
| ! | TCATTTTGTGTAGTTGTGGT+AGG | - | chr1_1:26347263-26347282 | None:intergenic | 35.0% |
| !!! | GTTTGTTTTGTGGGATTGTT+TGG | - | chr1_1:26347301-26347320 | None:intergenic | 35.0% |
| !!! | CTTGTGCTTGTTTGTTTTGT+GGG | - | chr1_1:26347310-26347329 | None:intergenic | 35.0% |
| !!! | TCTTGTGCTTGTTTGTTTTG+TGG | - | chr1_1:26347311-26347330 | None:intergenic | 35.0% |
| TGAAAGATCTGCTAGAGAAG+TGG | - | chr1_1:26347159-26347178 | None:intergenic | 40.0% | |
| TTCTCTAGCAGATCTTTCAG+AGG | + | chr1_1:26347160-26347179 | Msa0015950:CDS | 40.0% | |
| ! | TTTTGTGTAGTTGTGGTAGG+AGG | - | chr1_1:26347260-26347279 | None:intergenic | 40.0% |
| AGAAGACCATACCATAGTTG+AGG | + | chr1_1:26347328-26347347 | Msa0015950:CDS | 40.0% | |
| GACTATCCTCAACTATGGTA+TGG | - | chr1_1:26347337-26347356 | None:intergenic | 40.0% | |
| CTTGTGACTATCCTCAACTA+TGG | - | chr1_1:26347342-26347361 | None:intergenic | 40.0% | |
| AAGATCTGCTAGAGAAGTGG+TGG | - | chr1_1:26347156-26347175 | None:intergenic | 45.0% | |
| CCAAGCATTAACACTCACAG+AGG | - | chr1_1:26347192-26347211 | None:intergenic | 45.0% | |
| CCTCTGTGAGTGTTAATGCT+TGG | + | chr1_1:26347189-26347208 | Msa0015950:CDS | 45.0% | |
| ! | TTTGGTGGCCAGTGTTTCTT+AGG | - | chr1_1:26347227-26347246 | None:intergenic | 45.0% |
| ! | GGGATTGTTTGGTCCACTTT+TGG | - | chr1_1:26347290-26347309 | None:intergenic | 45.0% |
| AAGGAGGATGCTTCTTCCAT+GGG | - | chr1_1:26347379-26347398 | None:intergenic | 45.0% | |
| ACTGGCCACCAAAGAAACAC+TGG | + | chr1_1:26347234-26347253 | Msa0015950:CDS | 50.0% | |
| GTAGGAGGCCAGTGTTTCTT+TGG | - | chr1_1:26347245-26347264 | None:intergenic | 50.0% | |
| GAAGGAGGATGCTTCTTCCA+TGG | - | chr1_1:26347380-26347399 | None:intergenic | 50.0% | |
| !! | TGGAAGAAGCATCCTCCTTC+AGG | + | chr1_1:26347380-26347399 | Msa0015950:CDS | 50.0% |
| GGAGGCCAGTGTTTCTTTGG+TGG | - | chr1_1:26347242-26347261 | None:intergenic | 55.0% | |
| AGCATCACCACAAGCACCCA+TGG | + | chr1_1:26347360-26347379 | Msa0015950:CDS | 55.0% | |
| GCTTCTTCCATGGGTGCTTG+TGG | - | chr1_1:26347370-26347389 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1_1 | gene | 26347101 | 26347409 | 26347101 | ID=Msa0015950;Name=Msa0015950 |
| chr1_1 | mRNA | 26347101 | 26347409 | 26347101 | ID=Msa0015950-mRNA-1;Parent=Msa0015950;Name=Msa0015950-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|102 |
| chr1_1 | exon | 26347101 | 26347409 | 26347101 | ID=Msa0015950-mRNA-1:exon:8916;Parent=Msa0015950-mRNA-1 |
| chr1_1 | CDS | 26347101 | 26347409 | 26347101 | ID=Msa0015950-mRNA-1:cds;Parent=Msa0015950-mRNA-1 |
| Gene Sequence |
| Protein sequence |
