AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0482650

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATGTTGTCCTAACAATTA+AGG 0.168807 3_3:-89866033 None:intergenic
TCAGTTTACCCTTAATTGTT+AGG 0.300983 3_3:+89866024 Msa0482650:three_prime_UTR
CATGATATGTTAGCCTGTTC+TGG 0.307236 3_3:+89865799 Msa0482650:three_prime_UTR
TAGATCTCTGTCTCATCTTT+CGG 0.343914 3_3:-89865725 None:intergenic
AAACTGAAAGCATAAATAAA+AGG 0.357989 3_3:-89866008 None:intergenic
TATGTTGTCCTAACAATTAA+GGG 0.364409 3_3:-89866032 None:intergenic
TATTGGTGCATGTACTGTTT+TGG 0.389852 3_3:+89865918 Msa0482650:three_prime_UTR
CTTTCCATCTTGCTTGATGC+CGG 0.419505 3_3:+89865773 Msa0482650:three_prime_UTR
ATCTTGAGGTTTGGTTGGGA+GGG 0.425741 3_3:+89865829 Msa0482650:three_prime_UTR
GATCTTGAGGTTTGGTTGGG+AGG 0.451658 3_3:+89865828 Msa0482650:three_prime_UTR
CAAGATCATTACACCAGAAC+AGG 0.453287 3_3:-89865812 None:intergenic
GCAAATTTCCGCGGCAGGGA+AGG 0.455538 3_3:-89864584 None:intergenic
TCTTGAGGTTTGGTTGGGAG+GGG 0.455633 3_3:+89865830 Msa0482650:three_prime_UTR
GGTGTAATGATCTTGAGGTT+TGG 0.457532 3_3:+89865820 Msa0482650:three_prime_UTR
TAATGATCTTGAGGTTTGGT+TGG 0.461100 3_3:+89865824 Msa0482650:three_prime_UTR
CGATGGCAAATTTCCGCGGC+AGG 0.470724 3_3:-89864589 None:intergenic
AATGATCTTGAGGTTTGGTT+GGG 0.481359 3_3:+89865825 Msa0482650:three_prime_UTR
ATGAAAATCATGTGGATGAG+GGG 0.489922 3_3:+89866144 Msa0482650:three_prime_UTR
ACATGAAAATCATGTGGATG+AGG 0.501805 3_3:+89866142 Msa0482650:three_prime_UTR
AGCGTATGAGGCGGTTGAAG+AGG 0.522163 3_3:+89865697 Msa0482650:CDS
GTTCTGGTGTAATGATCTTG+AGG 0.522478 3_3:+89865815 Msa0482650:three_prime_UTR
AATTACCTTGGCTCTCATGT+TGG 0.522645 3_3:-89864645 None:intergenic
GATGAGACAGAGATCTAAGT+AGG 0.529953 3_3:+89865731 Msa0482650:exon
ATCTAAGTAGGTGCATATGC+AGG 0.532333 3_3:+89865743 Msa0482650:exon
TTCAGTTCCCTTCCCTGCCG+CGG 0.533826 3_3:+89864576 Msa0482650:CDS
AGTGGAAGAAGAAGCGTATG+AGG 0.535090 3_3:+89865685 Msa0482650:intron
CACTGGGTTAGCTTAAGATG+TGG 0.544623 3_3:+89866081 Msa0482650:three_prime_UTR
CATGAAAATCATGTGGATGA+GGG 0.544921 3_3:+89866143 Msa0482650:three_prime_UTR
CAAATTTCCGCGGCAGGGAA+GGG 0.545646 3_3:-89864583 None:intergenic
GAAGCCGGCATCAAGCAAGA+TGG 0.582153 3_3:-89865777 None:intergenic
AGGCTAACATATCATGAAGC+CGG 0.585518 3_3:-89865792 None:intergenic
GTGCTGTGTTTCGTAAGAAG+CGG 0.597751 3_3:-89864518 None:intergenic
ATCTTAAGCTAACCCAGTGA+AGG 0.623767 3_3:-89866077 None:intergenic
TGAAAATCATGTGGATGAGG+GGG 0.624026 3_3:+89866145 Msa0482650:three_prime_UTR
ATCATCCAACATGAGAGCCA+AGG 0.635896 3_3:+89864640 Msa0482650:CDS
CTGAAAGCATAAATAAAAGG+AGG 0.640654 3_3:-89866005 None:intergenic
TTAAATACATGAAAATCATG+TGG 0.661283 3_3:+89866136 Msa0482650:three_prime_UTR
GATGGCAAATTTCCGCGGCA+GGG 0.670700 3_3:-89864588 None:intergenic
CACTATAAATACGAAAACGA+GGG 0.686259 3_3:+89864538 Msa0482650:CDS
GCACTATAAATACGAAAACG+AGG 0.689830 3_3:+89864537 Msa0482650:CDS
TCTCAAGTTTCTCTGTACGA+TGG 0.694510 3_3:-89864606 None:intergenic
TCAATTGTCTTAGAACAACA+AGG 0.700588 3_3:-89865878 None:intergenic
GGAAGAAGAAGCGTATGAGG+CGG 0.708289 3_3:+89865688 Msa0482650:CDS
TGTACGATGGCAAATTTCCG+CGG 0.709054 3_3:-89864593 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAAATGTTCAATATTTT+TGG + chr3_3:89864723-89864742 Msa0482650:intron 10.0%
!!! TTTAAATGTTCAATATTTTT+GGG + chr3_3:89864724-89864743 Msa0482650:intron 10.0%
!! AAAAAATATTATTCATTTCT+GGG - chr3_3:89865050-89865069 None:intergenic 10.0%
!!! TTTTAAATGTTCAATATTTT+TGG + chr3_3:89864723-89864742 Msa0482650:intron 10.0%
!!! TTTAAATGTTCAATATTTTT+GGG + chr3_3:89864724-89864743 Msa0482650:intron 10.0%
!! AAAAAATATTATTCATTTCT+GGG - chr3_3:89865050-89865069 None:intergenic 10.0%
!! ATTAGATACAATTATGATTT+AGG + chr3_3:89864747-89864766 Msa0482650:intron 15.0%
!! TTAGATACAATTATGATTTA+GGG + chr3_3:89864748-89864767 Msa0482650:intron 15.0%
!! GAAAAAATATTATTCATTTC+TGG - chr3_3:89865051-89865070 None:intergenic 15.0%
!! TAAATTAAATGTGATTGTAT+TGG + chr3_3:89865154-89865173 Msa0482650:intron 15.0%
!! ATTAGATACAATTATGATTT+AGG + chr3_3:89864747-89864766 Msa0482650:intron 15.0%
!! TTAGATACAATTATGATTTA+GGG + chr3_3:89864748-89864767 Msa0482650:intron 15.0%
!! GAAAAAATATTATTCATTTC+TGG - chr3_3:89865051-89865070 None:intergenic 15.0%
!! TAAATTAAATGTGATTGTAT+TGG + chr3_3:89865154-89865173 Msa0482650:intron 15.0%
!! TTTATATAATCAATCGAAGA+AGG - chr3_3:89864674-89864693 None:intergenic 20.0%
!! AAAACACATCAAACATTAAA+CGG - chr3_3:89864802-89864821 None:intergenic 20.0%
!!! AGTCAATTTGATGATTTTTT+AGG + chr3_3:89864928-89864947 Msa0482650:intron 20.0%
!! ATCACTGTTTAAAAATACTT+GGG - chr3_3:89865076-89865095 None:intergenic 20.0%
!! AATCACTGTTTAAAAATACT+TGG - chr3_3:89865077-89865096 None:intergenic 20.0%
!!! AAACTGAAAGCATAAATAAA+AGG - chr3_3:89866011-89866030 None:intergenic 20.0%
!! TTAAATACATGAAAATCATG+TGG + chr3_3:89866136-89866155 Msa0482650:three_prime_UTR 20.0%
!! TTTATATAATCAATCGAAGA+AGG - chr3_3:89864674-89864693 None:intergenic 20.0%
!! AAAACACATCAAACATTAAA+CGG - chr3_3:89864802-89864821 None:intergenic 20.0%
!!! AGTCAATTTGATGATTTTTT+AGG + chr3_3:89864928-89864947 Msa0482650:intron 20.0%
!! ATCACTGTTTAAAAATACTT+GGG - chr3_3:89865076-89865095 None:intergenic 20.0%
!! AATCACTGTTTAAAAATACT+TGG - chr3_3:89865077-89865096 None:intergenic 20.0%
!!! AAACTGAAAGCATAAATAAA+AGG - chr3_3:89866011-89866030 None:intergenic 20.0%
!! TTAAATACATGAAAATCATG+TGG + chr3_3:89866136-89866155 Msa0482650:three_prime_UTR 20.0%
!! TACAATTATGATTTAGGGTT+TGG + chr3_3:89864753-89864772 Msa0482650:intron 25.0%
!! ACAATTATGATTTAGGGTTT+GGG + chr3_3:89864754-89864773 Msa0482650:intron 25.0%
! TTCACATAATCATTTCTATG+AGG - chr3_3:89864845-89864864 None:intergenic 25.0%
! ATAATAGCGGTAATTATCTA+TGG - chr3_3:89865188-89865207 None:intergenic 25.0%
!!! TGAGTTTTAACTGATAATAG+CGG - chr3_3:89865201-89865220 None:intergenic 25.0%
! CTTAAGAAATTACACAATCT+CGG - chr3_3:89865286-89865305 None:intergenic 25.0%
! TATATCATAAGGTTACAACA+TGG - chr3_3:89865355-89865374 None:intergenic 25.0%
! CATACAGAGTATATATCATA+AGG - chr3_3:89865366-89865385 None:intergenic 25.0%
! TATGATATATACTCTGTATG+CGG + chr3_3:89865366-89865385 Msa0482650:intron 25.0%
!!! CATTTTGTGGTTTTTAAGTA+GGG + chr3_3:89865427-89865446 Msa0482650:intron 25.0%
!!! TATGTATTTTTTTCCCATTG+TGG + chr3_3:89865595-89865614 Msa0482650:intron 25.0%
!!! TTTTGAGTGTTTTGTAGTAT+TGG + chr3_3:89865901-89865920 Msa0482650:three_prime_UTR 25.0%
! TATGTTGTCCTAACAATTAA+GGG - chr3_3:89866035-89866054 None:intergenic 25.0%
! ATATGTTGTCCTAACAATTA+AGG - chr3_3:89866036-89866055 None:intergenic 25.0%
!! TACAATTATGATTTAGGGTT+TGG + chr3_3:89864753-89864772 Msa0482650:intron 25.0%
!! ACAATTATGATTTAGGGTTT+GGG + chr3_3:89864754-89864773 Msa0482650:intron 25.0%
! TTCACATAATCATTTCTATG+AGG - chr3_3:89864845-89864864 None:intergenic 25.0%
! ATAATAGCGGTAATTATCTA+TGG - chr3_3:89865188-89865207 None:intergenic 25.0%
!!! TGAGTTTTAACTGATAATAG+CGG - chr3_3:89865201-89865220 None:intergenic 25.0%
! CTTAAGAAATTACACAATCT+CGG - chr3_3:89865286-89865305 None:intergenic 25.0%
! TATATCATAAGGTTACAACA+TGG - chr3_3:89865355-89865374 None:intergenic 25.0%
! CATACAGAGTATATATCATA+AGG - chr3_3:89865366-89865385 None:intergenic 25.0%
! TATGATATATACTCTGTATG+CGG + chr3_3:89865366-89865385 Msa0482650:intron 25.0%
!!! CATTTTGTGGTTTTTAAGTA+GGG + chr3_3:89865427-89865446 Msa0482650:intron 25.0%
!!! TATGTATTTTTTTCCCATTG+TGG + chr3_3:89865595-89865614 Msa0482650:intron 25.0%
!!! TTTTGAGTGTTTTGTAGTAT+TGG + chr3_3:89865901-89865920 Msa0482650:three_prime_UTR 25.0%
! TATGTTGTCCTAACAATTAA+GGG - chr3_3:89866035-89866054 None:intergenic 25.0%
! ATATGTTGTCCTAACAATTA+AGG - chr3_3:89866036-89866055 None:intergenic 25.0%
CACTATAAATACGAAAACGA+GGG + chr3_3:89864538-89864557 Msa0482650:CDS 30.0%
! ACAACCATGTTTCTATCATT+TGG - chr3_3:89864885-89864904 None:intergenic 30.0%
ACTGTTTAAAAATACTTGGG+CGG - chr3_3:89865073-89865092 None:intergenic 30.0%
AATTTATGACCAATGAACCT+CGG - chr3_3:89865121-89865140 None:intergenic 30.0%
CAATTCAGTTAATAAGCCAA+AGG - chr3_3:89865250-89865269 None:intergenic 30.0%
CGAGATTGTGTAATTTCTTA+AGG + chr3_3:89865284-89865303 Msa0482650:intron 30.0%
ACTTAAAAACCACAAAATGC+TGG - chr3_3:89865426-89865445 None:intergenic 30.0%
!!! GCATTTTGTGGTTTTTAAGT+AGG + chr3_3:89865426-89865445 Msa0482650:intron 30.0%
! CTTCATAACAGTATTTTGCA+TGG + chr3_3:89865522-89865541 Msa0482650:intron 30.0%
TTCATAACAGTATCATGCTT+TGG + chr3_3:89865562-89865581 Msa0482650:intron 30.0%
TCAATGATGTTATGTTTCAG+TGG + chr3_3:89865667-89865686 Msa0482650:intron 30.0%
TCAATTGTCTTAGAACAACA+AGG - chr3_3:89865881-89865900 None:intergenic 30.0%
! CTGAAAGCATAAATAAAAGG+AGG - chr3_3:89866008-89866027 None:intergenic 30.0%
TCAGTTTACCCTTAATTGTT+AGG + chr3_3:89866024-89866043 Msa0482650:three_prime_UTR 30.0%
! ATTTTATGATATGCCTTCAC+TGG + chr3_3:89866064-89866083 Msa0482650:three_prime_UTR 30.0%
! TTTTATGATATGCCTTCACT+GGG + chr3_3:89866065-89866084 Msa0482650:three_prime_UTR 30.0%
CACTATAAATACGAAAACGA+GGG + chr3_3:89864538-89864557 Msa0482650:CDS 30.0%
! ACAACCATGTTTCTATCATT+TGG - chr3_3:89864885-89864904 None:intergenic 30.0%
ACTGTTTAAAAATACTTGGG+CGG - chr3_3:89865073-89865092 None:intergenic 30.0%
AATTTATGACCAATGAACCT+CGG - chr3_3:89865121-89865140 None:intergenic 30.0%
CAATTCAGTTAATAAGCCAA+AGG - chr3_3:89865250-89865269 None:intergenic 30.0%
CGAGATTGTGTAATTTCTTA+AGG + chr3_3:89865284-89865303 Msa0482650:intron 30.0%
ACTTAAAAACCACAAAATGC+TGG - chr3_3:89865426-89865445 None:intergenic 30.0%
!!! GCATTTTGTGGTTTTTAAGT+AGG + chr3_3:89865426-89865445 Msa0482650:intron 30.0%
! CTTCATAACAGTATTTTGCA+TGG + chr3_3:89865522-89865541 Msa0482650:intron 30.0%
TTCATAACAGTATCATGCTT+TGG + chr3_3:89865562-89865581 Msa0482650:intron 30.0%
TCAATGATGTTATGTTTCAG+TGG + chr3_3:89865667-89865686 Msa0482650:intron 30.0%
TCAATTGTCTTAGAACAACA+AGG - chr3_3:89865881-89865900 None:intergenic 30.0%
! CTGAAAGCATAAATAAAAGG+AGG - chr3_3:89866008-89866027 None:intergenic 30.0%
TCAGTTTACCCTTAATTGTT+AGG + chr3_3:89866024-89866043 Msa0482650:three_prime_UTR 30.0%
! ATTTTATGATATGCCTTCAC+TGG + chr3_3:89866064-89866083 Msa0482650:three_prime_UTR 30.0%
! TTTTATGATATGCCTTCACT+GGG + chr3_3:89866065-89866084 Msa0482650:three_prime_UTR 30.0%
GCACTATAAATACGAAAACG+AGG + chr3_3:89864537-89864556 Msa0482650:CDS 35.0%
! ATGATTTAGGGTTTGGGTTT+AGG + chr3_3:89864760-89864779 Msa0482650:intron 35.0%
CGTTCCAAATGATAGAAACA+TGG + chr3_3:89864878-89864897 Msa0482650:intron 35.0%
TACTATAATCCGAGGTTCAT+TGG + chr3_3:89865109-89865128 Msa0482650:intron 35.0%
!!! TTTTTTCCCATTGTGGTTGT+TGG + chr3_3:89865602-89865621 Msa0482650:intron 35.0%
TAGATCTCTGTCTCATCTTT+CGG - chr3_3:89865728-89865747 None:intergenic 35.0%
! TAATGATCTTGAGGTTTGGT+TGG + chr3_3:89865824-89865843 Msa0482650:three_prime_UTR 35.0%
! AATGATCTTGAGGTTTGGTT+GGG + chr3_3:89865825-89865844 Msa0482650:three_prime_UTR 35.0%
! TATTGGTGCATGTACTGTTT+TGG + chr3_3:89865918-89865937 Msa0482650:three_prime_UTR 35.0%
ACATGAAAATCATGTGGATG+AGG + chr3_3:89866142-89866161 Msa0482650:three_prime_UTR 35.0%
CATGAAAATCATGTGGATGA+GGG + chr3_3:89866143-89866162 Msa0482650:three_prime_UTR 35.0%
ATGAAAATCATGTGGATGAG+GGG + chr3_3:89866144-89866163 Msa0482650:three_prime_UTR 35.0%
GCACTATAAATACGAAAACG+AGG + chr3_3:89864537-89864556 Msa0482650:CDS 35.0%
! ATGATTTAGGGTTTGGGTTT+AGG + chr3_3:89864760-89864779 Msa0482650:intron 35.0%
CGTTCCAAATGATAGAAACA+TGG + chr3_3:89864878-89864897 Msa0482650:intron 35.0%
TACTATAATCCGAGGTTCAT+TGG + chr3_3:89865109-89865128 Msa0482650:intron 35.0%
!!! TTTTTTCCCATTGTGGTTGT+TGG + chr3_3:89865602-89865621 Msa0482650:intron 35.0%
TAGATCTCTGTCTCATCTTT+CGG - chr3_3:89865728-89865747 None:intergenic 35.0%
! TAATGATCTTGAGGTTTGGT+TGG + chr3_3:89865824-89865843 Msa0482650:three_prime_UTR 35.0%
! AATGATCTTGAGGTTTGGTT+GGG + chr3_3:89865825-89865844 Msa0482650:three_prime_UTR 35.0%
! TATTGGTGCATGTACTGTTT+TGG + chr3_3:89865918-89865937 Msa0482650:three_prime_UTR 35.0%
ACATGAAAATCATGTGGATG+AGG + chr3_3:89866142-89866161 Msa0482650:three_prime_UTR 35.0%
CATGAAAATCATGTGGATGA+GGG + chr3_3:89866143-89866162 Msa0482650:three_prime_UTR 35.0%
ATGAAAATCATGTGGATGAG+GGG + chr3_3:89866144-89866163 Msa0482650:three_prime_UTR 35.0%
TCTCAAGTTTCTCTGTACGA+TGG - chr3_3:89864609-89864628 None:intergenic 40.0%
! AATTACCTTGGCTCTCATGT+TGG - chr3_3:89864648-89864667 None:intergenic 40.0%
CGAAGAAGGATGAATTACCT+TGG - chr3_3:89864660-89864679 None:intergenic 40.0%
TGACAGTGTACTATAATCCG+AGG + chr3_3:89865101-89865120 Msa0482650:intron 40.0%
ACACAATCTCGGCATCTTAA+TGG - chr3_3:89865275-89865294 None:intergenic 40.0%
GATATATACTCTGTATGCGG+AGG + chr3_3:89865369-89865388 Msa0482650:intron 40.0%
!!! TGGTTTTTAAGTAGGGCTAG+TGG + chr3_3:89865434-89865453 Msa0482650:intron 40.0%
TGTCAATGACTACCGTAGAA+TGG - chr3_3:89865499-89865518 None:intergenic 40.0%
ATCAAGCCAACAACCACAAT+GGG - chr3_3:89865611-89865630 None:intergenic 40.0%
AATCAAGCCAACAACCACAA+TGG - chr3_3:89865612-89865631 None:intergenic 40.0%
GATGAGACAGAGATCTAAGT+AGG + chr3_3:89865731-89865750 Msa0482650:exon 40.0%
ATCTAAGTAGGTGCATATGC+AGG + chr3_3:89865743-89865762 Msa0482650:exon 40.0%
AGGCTAACATATCATGAAGC+CGG - chr3_3:89865795-89865814 None:intergenic 40.0%
! CATGATATGTTAGCCTGTTC+TGG + chr3_3:89865799-89865818 Msa0482650:three_prime_UTR 40.0%
CAAGATCATTACACCAGAAC+AGG - chr3_3:89865815-89865834 None:intergenic 40.0%
GTTCTGGTGTAATGATCTTG+AGG + chr3_3:89865815-89865834 Msa0482650:three_prime_UTR 40.0%
GGTGTAATGATCTTGAGGTT+TGG + chr3_3:89865820-89865839 Msa0482650:three_prime_UTR 40.0%
ATCTTAAGCTAACCCAGTGA+AGG - chr3_3:89866080-89866099 None:intergenic 40.0%
TGAAAATCATGTGGATGAGG+GGG + chr3_3:89866145-89866164 Msa0482650:three_prime_UTR 40.0%
TCTCAAGTTTCTCTGTACGA+TGG - chr3_3:89864609-89864628 None:intergenic 40.0%
! AATTACCTTGGCTCTCATGT+TGG - chr3_3:89864648-89864667 None:intergenic 40.0%
CGAAGAAGGATGAATTACCT+TGG - chr3_3:89864660-89864679 None:intergenic 40.0%
TGACAGTGTACTATAATCCG+AGG + chr3_3:89865101-89865120 Msa0482650:intron 40.0%
ACACAATCTCGGCATCTTAA+TGG - chr3_3:89865275-89865294 None:intergenic 40.0%
GATATATACTCTGTATGCGG+AGG + chr3_3:89865369-89865388 Msa0482650:intron 40.0%
!!! TGGTTTTTAAGTAGGGCTAG+TGG + chr3_3:89865434-89865453 Msa0482650:intron 40.0%
TGTCAATGACTACCGTAGAA+TGG - chr3_3:89865499-89865518 None:intergenic 40.0%
ATCAAGCCAACAACCACAAT+GGG - chr3_3:89865611-89865630 None:intergenic 40.0%
AATCAAGCCAACAACCACAA+TGG - chr3_3:89865612-89865631 None:intergenic 40.0%
GATGAGACAGAGATCTAAGT+AGG + chr3_3:89865731-89865750 Msa0482650:exon 40.0%
ATCTAAGTAGGTGCATATGC+AGG + chr3_3:89865743-89865762 Msa0482650:exon 40.0%
AGGCTAACATATCATGAAGC+CGG - chr3_3:89865795-89865814 None:intergenic 40.0%
! CATGATATGTTAGCCTGTTC+TGG + chr3_3:89865799-89865818 Msa0482650:three_prime_UTR 40.0%
CAAGATCATTACACCAGAAC+AGG - chr3_3:89865815-89865834 None:intergenic 40.0%
GTTCTGGTGTAATGATCTTG+AGG + chr3_3:89865815-89865834 Msa0482650:three_prime_UTR 40.0%
GGTGTAATGATCTTGAGGTT+TGG + chr3_3:89865820-89865839 Msa0482650:three_prime_UTR 40.0%
ATCTTAAGCTAACCCAGTGA+AGG - chr3_3:89866080-89866099 None:intergenic 40.0%
TGAAAATCATGTGGATGAGG+GGG + chr3_3:89866145-89866164 Msa0482650:three_prime_UTR 40.0%
GTGCTGTGTTTCGTAAGAAG+CGG - chr3_3:89864521-89864540 None:intergenic 45.0%
TGTACGATGGCAAATTTCCG+CGG - chr3_3:89864596-89864615 None:intergenic 45.0%
ATCATCCAACATGAGAGCCA+AGG + chr3_3:89864640-89864659 Msa0482650:CDS 45.0%
! GTCTTTGAGCCAGCATTTTG+TGG + chr3_3:89865414-89865433 Msa0482650:intron 45.0%
CCTTCCTGATAGCCATTCTA+CGG + chr3_3:89865484-89865503 Msa0482650:intron 45.0%
ACTACCGTAGAATGGCTATC+AGG - chr3_3:89865491-89865510 None:intergenic 45.0%
! AGTGGAAGAAGAAGCGTATG+AGG + chr3_3:89865685-89865704 Msa0482650:intron 45.0%
CTTTCCATCTTGCTTGATGC+CGG + chr3_3:89865773-89865792 Msa0482650:three_prime_UTR 45.0%
! ATCTTGAGGTTTGGTTGGGA+GGG + chr3_3:89865829-89865848 Msa0482650:three_prime_UTR 45.0%
!! TGGTGCATGTACTGTTTTGG+TGG + chr3_3:89865921-89865940 Msa0482650:three_prime_UTR 45.0%
!! CACTGGGTTAGCTTAAGATG+TGG + chr3_3:89866081-89866100 Msa0482650:three_prime_UTR 45.0%
GTGCTGTGTTTCGTAAGAAG+CGG - chr3_3:89864521-89864540 None:intergenic 45.0%
TGTACGATGGCAAATTTCCG+CGG - chr3_3:89864596-89864615 None:intergenic 45.0%
ATCATCCAACATGAGAGCCA+AGG + chr3_3:89864640-89864659 Msa0482650:CDS 45.0%
! GTCTTTGAGCCAGCATTTTG+TGG + chr3_3:89865414-89865433 Msa0482650:intron 45.0%
CCTTCCTGATAGCCATTCTA+CGG + chr3_3:89865484-89865503 Msa0482650:intron 45.0%
ACTACCGTAGAATGGCTATC+AGG - chr3_3:89865491-89865510 None:intergenic 45.0%
! AGTGGAAGAAGAAGCGTATG+AGG + chr3_3:89865685-89865704 Msa0482650:intron 45.0%
CTTTCCATCTTGCTTGATGC+CGG + chr3_3:89865773-89865792 Msa0482650:three_prime_UTR 45.0%
! ATCTTGAGGTTTGGTTGGGA+GGG + chr3_3:89865829-89865848 Msa0482650:three_prime_UTR 45.0%
!! TGGTGCATGTACTGTTTTGG+TGG + chr3_3:89865921-89865940 Msa0482650:three_prime_UTR 45.0%
!! CACTGGGTTAGCTTAAGATG+TGG + chr3_3:89866081-89866100 Msa0482650:three_prime_UTR 45.0%
! ATAAGCCAAAGGCGCAATGC+TGG - chr3_3:89865239-89865258 None:intergenic 50.0%
CCGTAGAATGGCTATCAGGA+AGG - chr3_3:89865487-89865506 None:intergenic 50.0%
! GGAAGAAGAAGCGTATGAGG+CGG + chr3_3:89865688-89865707 Msa0482650:CDS 50.0%
! GATCTTGAGGTTTGGTTGGG+AGG + chr3_3:89865828-89865847 Msa0482650:three_prime_UTR 50.0%
! TCTTGAGGTTTGGTTGGGAG+GGG + chr3_3:89865830-89865849 Msa0482650:three_prime_UTR 50.0%
GAGGGGGCAAAAATGCTTTC+AGG + chr3_3:89866161-89866180 Msa0482650:three_prime_UTR 50.0%
! ATAAGCCAAAGGCGCAATGC+TGG - chr3_3:89865239-89865258 None:intergenic 50.0%
CCGTAGAATGGCTATCAGGA+AGG - chr3_3:89865487-89865506 None:intergenic 50.0%
! GGAAGAAGAAGCGTATGAGG+CGG + chr3_3:89865688-89865707 Msa0482650:CDS 50.0%
! GATCTTGAGGTTTGGTTGGG+AGG + chr3_3:89865828-89865847 Msa0482650:three_prime_UTR 50.0%
! TCTTGAGGTTTGGTTGGGAG+GGG + chr3_3:89865830-89865849 Msa0482650:three_prime_UTR 50.0%
CAAATTTCCGCGGCAGGGAA+GGG - chr3_3:89864586-89864605 None:intergenic 55.0%
GATGGCAAATTTCCGCGGCA+GGG - chr3_3:89864591-89864610 None:intergenic 55.0%
TGCTACCAGCATTGCGCCTT+TGG + chr3_3:89865231-89865250 Msa0482650:intron 55.0%
AGCGTATGAGGCGGTTGAAG+AGG + chr3_3:89865697-89865716 Msa0482650:CDS 55.0%
GAAGCCGGCATCAAGCAAGA+TGG - chr3_3:89865780-89865799 None:intergenic 55.0%
CAAATTTCCGCGGCAGGGAA+GGG - chr3_3:89864586-89864605 None:intergenic 55.0%
GATGGCAAATTTCCGCGGCA+GGG - chr3_3:89864591-89864610 None:intergenic 55.0%
TGCTACCAGCATTGCGCCTT+TGG + chr3_3:89865231-89865250 Msa0482650:intron 55.0%
AGCGTATGAGGCGGTTGAAG+AGG + chr3_3:89865697-89865716 Msa0482650:CDS 55.0%
GAAGCCGGCATCAAGCAAGA+TGG - chr3_3:89865780-89865799 None:intergenic 55.0%
TTCAGTTCCCTTCCCTGCCG+CGG + chr3_3:89864576-89864595 Msa0482650:CDS 60.0%
GCAAATTTCCGCGGCAGGGA+AGG - chr3_3:89864587-89864606 None:intergenic 60.0%
CGATGGCAAATTTCCGCGGC+AGG - chr3_3:89864592-89864611 None:intergenic 60.0%
TTCAGTTCCCTTCCCTGCCG+CGG + chr3_3:89864576-89864595 Msa0482650:CDS 60.0%
GCAAATTTCCGCGGCAGGGA+AGG - chr3_3:89864587-89864606 None:intergenic 60.0%
CGATGGCAAATTTCCGCGGC+AGG - chr3_3:89864592-89864611 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 89864514 89866181 89864514 ID=Msa0482650;Name=Msa0482650
chr3_3 mRNA 89864514 89866181 89864514 ID=Msa0482650-mRNA-1;Parent=Msa0482650;Name=Msa0482650-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|1|0.5|1|1|1|2|433|69
chr3_3 exon 89864514 89864661 89864514 ID=Msa0482650-mRNA-1:exon:26233;Parent=Msa0482650-mRNA-1
chr3_3 exon 89865687 89866181 89865687 ID=Msa0482650-mRNA-1:exon:26234;Parent=Msa0482650-mRNA-1
chr3_3 CDS 89864514 89864661 89864514 ID=Msa0482650-mRNA-1:cds;Parent=Msa0482650-mRNA-1
chr3_3 CDS 89865687 89865748 89865687 ID=Msa0482650-mRNA-1:cds;Parent=Msa0482650-mRNA-1
chr3_3 three_prime_UTR 89865749 89866181 89865749 ID=Msa0482650-mRNA-1:three_prime_utr;Parent=Msa0482650-mRNA-1
Gene Sequence

>Msa0482650

GGCTCCGCTTCTTACGAAACACAGCACTATAAATACGAAAACGAGGGTTTTTCACTCTCAGATTCAGTTCCCTTCCCTGCCGCGGAAATTTGCCATCGTACAGAGAAACTTGAGAGCGTAGCGTTCATCATCCAACATGAGAGCCAAGTGGAAGAAGAAGCGTATGAGGCGGTTGAAGAGGAAGCGCCGAAAGATGAGACAGAGATCTAA
Protein sequence

>Msa0482650

GSASYETQHYKYENEGFSLSDSVPFPAAEICHRTEKLESVAFIIQHESQVEEEAYEAVEEEAPKDETEI*