AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0148060


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0148060 MtrunA17_Chr1g0151021 100.000 48 0 0 1 48 1 48 3.83e-30 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0148060 AT5G18790.1 73.469 49 12 1 1 48 1 49 3.68e-19 73.6
Msa0148060 AT3G06320.1 73.469 49 12 1 1 48 1 49 3.68e-19 73.6

Find 19 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAATCATGATGGTCATTC+AGG 0.251774 1_4:+8266758 Msa0148060:CDS
CTATATTGAGGGTCTTAAAT+TGG 0.270437 1_4:+8266736 Msa0148060:CDS
CTTGTGTCTGCTGCGGGGAC+TGG 0.330521 1_4:+8265650 Msa0148060:CDS
CTTTCGAAACTCAAGTTTCT+CGG 0.360033 1_4:-8265708 None:intergenic
CTGGATTCTTCTATGTTAAG+AGG 0.413275 1_4:+8265669 Msa0148060:CDS
GTGAAACTTGTGTCTGCTGC+GGG 0.420813 1_4:+8265644 Msa0148060:CDS
AGTTTCTCGGTAAACTGTCT+TGG 0.447698 1_4:-8265695 None:intergenic
CTAATCATGATGGTCATTCA+GGG 0.476963 1_4:+8266759 Msa0148060:CDS
CAGCACATGACGATTAACTC+TGG 0.494495 1_4:-8265738 None:intergenic
ATCTTATATAGAAGAGATGT+AGG 0.499104 1_4:+8266708 Msa0148060:intron
ATGGTCATTCAGGGTTTGAA+AGG 0.515395 1_4:+8266768 Msa0148060:CDS
ATGTAGGGAGCTCTATATTG+AGG 0.518117 1_4:+8266724 Msa0148060:CDS
CTTAAATTGGCTAATCATGA+TGG 0.538841 1_4:+8266749 Msa0148060:CDS
TGTAGGGAGCTCTATATTGA+GGG 0.543050 1_4:+8266725 Msa0148060:CDS
AATGGGTAACGCTAAGAAGA+AGG 0.544811 1_4:+8265610 None:intergenic
CGTGAAACTTGTGTCTGCTG+CGG 0.559210 1_4:+8265643 Msa0148060:CDS
AGCACATGACGATTAACTCT+GGG 0.592000 1_4:-8265737 None:intergenic
TCTTATATAGAAGAGATGTA+GGG 0.607516 1_4:+8266709 Msa0148060:intron
TGAAACTTGTGTCTGCTGCG+GGG 0.708413 1_4:+8265645 Msa0148060:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTTCATCAATATATATTT+TGG - chr1_4:8265973-8265992 None:intergenic 15.0%
!!! GTTTTTTTTTTTTTTTGGAA+CGG + chr1_4:8266003-8266022 Msa0148060:intron 15.0%
!! AAAATATCACAATAAAAAAC+TGG - chr1_4:8266306-8266325 None:intergenic 15.0%
!! TGCATATTAAAATTATATAC+AGG - chr1_4:8266339-8266358 None:intergenic 15.0%
!!! TTTTAAATTAAAATTTGAGG+AGG + chr1_4:8266369-8266388 Msa0148060:intron 15.0%
!!! GATTTCATCAATATATATTT+TGG - chr1_4:8265973-8265992 None:intergenic 15.0%
!!! GTTTTTTTTTTTTTTTGGAA+CGG + chr1_4:8266003-8266022 Msa0148060:intron 15.0%
!! AAAATATCACAATAAAAAAC+TGG - chr1_4:8266306-8266325 None:intergenic 15.0%
!! TGCATATTAAAATTATATAC+AGG - chr1_4:8266339-8266358 None:intergenic 15.0%
!!! TTTTAAATTAAAATTTGAGG+AGG + chr1_4:8266369-8266388 Msa0148060:intron 15.0%
!! ATTATCACATTCTATATTAC+AGG + chr1_4:8265840-8265859 Msa0148060:intron 20.0%
!! TCAACAAACAATAAAAAGAT+TGG - chr1_4:8265876-8265895 None:intergenic 20.0%
!!! CAATATATATTTTGGAAAGT+TGG - chr1_4:8265965-8265984 None:intergenic 20.0%
!!! TATTGATGAAATCTTATTTC+TGG + chr1_4:8265980-8265999 Msa0148060:intron 20.0%
!!! ATTGATGAAATCTTATTTCT+GGG + chr1_4:8265981-8266000 Msa0148060:intron 20.0%
!!! TCTGGGTTTTTTTTTTTTTT+TGG + chr1_4:8265998-8266017 Msa0148060:intron 20.0%
!!! TCTAACGACTTTAAAAATTA+GGG - chr1_4:8266051-8266070 None:intergenic 20.0%
!!! ATCTAACGACTTTAAAAATT+AGG - chr1_4:8266052-8266071 None:intergenic 20.0%
!! ATGAGTAAATATAAGAACAA+GGG - chr1_4:8266262-8266281 None:intergenic 20.0%
!!! GTTTAAAATTCTTTCAATTG+TGG - chr1_4:8266579-8266598 None:intergenic 20.0%
!! ATTATCACATTCTATATTAC+AGG + chr1_4:8265840-8265859 Msa0148060:intron 20.0%
!! TCAACAAACAATAAAAAGAT+TGG - chr1_4:8265876-8265895 None:intergenic 20.0%
!!! CAATATATATTTTGGAAAGT+TGG - chr1_4:8265965-8265984 None:intergenic 20.0%
!!! TATTGATGAAATCTTATTTC+TGG + chr1_4:8265980-8265999 Msa0148060:intron 20.0%
!!! ATTGATGAAATCTTATTTCT+GGG + chr1_4:8265981-8266000 Msa0148060:intron 20.0%
!!! TCTGGGTTTTTTTTTTTTTT+TGG + chr1_4:8265998-8266017 Msa0148060:intron 20.0%
!!! TCTAACGACTTTAAAAATTA+GGG - chr1_4:8266051-8266070 None:intergenic 20.0%
!!! ATCTAACGACTTTAAAAATT+AGG - chr1_4:8266052-8266071 None:intergenic 20.0%
!! ATGAGTAAATATAAGAACAA+GGG - chr1_4:8266262-8266281 None:intergenic 20.0%
!!! GTTTAAAATTCTTTCAATTG+TGG - chr1_4:8266579-8266598 None:intergenic 20.0%
! TGTTAGTATGCAGTATTATT+TGG + chr1_4:8265903-8265922 Msa0148060:intron 25.0%
! GTTAGTATGCAGTATTATTT+GGG + chr1_4:8265904-8265923 Msa0148060:intron 25.0%
! ACTCATCTAAATTCATTTCT+AGG + chr1_4:8265938-8265957 Msa0148060:intron 25.0%
!!! TTTTTTTTTTTTGGAACGGT+TGG + chr1_4:8266007-8266026 Msa0148060:intron 25.0%
!!! TTTTTTTTTTTGGAACGGTT+GGG + chr1_4:8266008-8266027 Msa0148060:intron 25.0%
! CCATATATATGAATCATAGA+AGG - chr1_4:8266159-8266178 None:intergenic 25.0%
! CCTTCTATGATTCATATATA+TGG + chr1_4:8266156-8266175 Msa0148060:intron 25.0%
!!! ACATCATTTGAAGCTTTATT+CGG + chr1_4:8266226-8266245 Msa0148060:intron 25.0%
! ATAAGAACAAGGGTAATTAA+TGG - chr1_4:8266252-8266271 None:intergenic 25.0%
! CATGAGTAAATATAAGAACA+AGG - chr1_4:8266263-8266282 None:intergenic 25.0%
! CTTGTTCTTATATTTACTCA+TGG + chr1_4:8266261-8266280 Msa0148060:intron 25.0%
! TTCAAGCAAAATTGTACTTT+GGG - chr1_4:8266465-8266484 None:intergenic 25.0%
! TTTCAAGCAAAATTGTACTT+TGG - chr1_4:8266466-8266485 None:intergenic 25.0%
! AATGAAAGAGCAATAATCTT+TGG + chr1_4:8266484-8266503 Msa0148060:intron 25.0%
! ATGAAAGAGCAATAATCTTT+GGG + chr1_4:8266485-8266504 Msa0148060:intron 25.0%
!! GAATTTTAAACCAATTTGCA+TGG + chr1_4:8266588-8266607 Msa0148060:intron 25.0%
! CATCTCTTCTATATAAGATA+AGG - chr1_4:8266707-8266726 None:intergenic 25.0%
! ATCTTATATAGAAGAGATGT+AGG + chr1_4:8266708-8266727 Msa0148060:intron 25.0%
! TCTTATATAGAAGAGATGTA+GGG + chr1_4:8266709-8266728 Msa0148060:intron 25.0%
! TGTTAGTATGCAGTATTATT+TGG + chr1_4:8265903-8265922 Msa0148060:intron 25.0%
! GTTAGTATGCAGTATTATTT+GGG + chr1_4:8265904-8265923 Msa0148060:intron 25.0%
! ACTCATCTAAATTCATTTCT+AGG + chr1_4:8265938-8265957 Msa0148060:intron 25.0%
!!! TTTTTTTTTTTTGGAACGGT+TGG + chr1_4:8266007-8266026 Msa0148060:intron 25.0%
!!! TTTTTTTTTTTGGAACGGTT+GGG + chr1_4:8266008-8266027 Msa0148060:intron 25.0%
! CCATATATATGAATCATAGA+AGG - chr1_4:8266159-8266178 None:intergenic 25.0%
! CCTTCTATGATTCATATATA+TGG + chr1_4:8266156-8266175 Msa0148060:intron 25.0%
!!! ACATCATTTGAAGCTTTATT+CGG + chr1_4:8266226-8266245 Msa0148060:intron 25.0%
! ATAAGAACAAGGGTAATTAA+TGG - chr1_4:8266252-8266271 None:intergenic 25.0%
! CATGAGTAAATATAAGAACA+AGG - chr1_4:8266263-8266282 None:intergenic 25.0%
! CTTGTTCTTATATTTACTCA+TGG + chr1_4:8266261-8266280 Msa0148060:intron 25.0%
! TTCAAGCAAAATTGTACTTT+GGG - chr1_4:8266465-8266484 None:intergenic 25.0%
! TTTCAAGCAAAATTGTACTT+TGG - chr1_4:8266466-8266485 None:intergenic 25.0%
! AATGAAAGAGCAATAATCTT+TGG + chr1_4:8266484-8266503 Msa0148060:intron 25.0%
! ATGAAAGAGCAATAATCTTT+GGG + chr1_4:8266485-8266504 Msa0148060:intron 25.0%
!! GAATTTTAAACCAATTTGCA+TGG + chr1_4:8266588-8266607 Msa0148060:intron 25.0%
! CATCTCTTCTATATAAGATA+AGG - chr1_4:8266707-8266726 None:intergenic 25.0%
! ATCTTATATAGAAGAGATGT+AGG + chr1_4:8266708-8266727 Msa0148060:intron 25.0%
! TCTTATATAGAAGAGATGTA+GGG + chr1_4:8266709-8266728 Msa0148060:intron 25.0%
! AGAAGTACAAGATTGTTACA+AGG - chr1_4:8265810-8265829 None:intergenic 30.0%
ATATACCTTCACATGCATAT+AGG - chr1_4:8266534-8266553 None:intergenic 30.0%
! TATATGCATGTGAAGGTATA+TGG + chr1_4:8266533-8266552 Msa0148060:intron 30.0%
!! GAAGTAACCCATGTTTTAAA+TGG + chr1_4:8266678-8266697 Msa0148060:intron 30.0%
! AAGGCAACCATTTAAAACAT+GGG - chr1_4:8266688-8266707 None:intergenic 30.0%
! TAAGGCAACCATTTAAAACA+TGG - chr1_4:8266689-8266708 None:intergenic 30.0%
CTATATTGAGGGTCTTAAAT+TGG + chr1_4:8266736-8266755 Msa0148060:CDS 30.0%
CTTAAATTGGCTAATCATGA+TGG + chr1_4:8266749-8266768 Msa0148060:CDS 30.0%
! AGAAGTACAAGATTGTTACA+AGG - chr1_4:8265810-8265829 None:intergenic 30.0%
ATATACCTTCACATGCATAT+AGG - chr1_4:8266534-8266553 None:intergenic 30.0%
! TATATGCATGTGAAGGTATA+TGG + chr1_4:8266533-8266552 Msa0148060:intron 30.0%
!! GAAGTAACCCATGTTTTAAA+TGG + chr1_4:8266678-8266697 Msa0148060:intron 30.0%
! AAGGCAACCATTTAAAACAT+GGG - chr1_4:8266688-8266707 None:intergenic 30.0%
! TAAGGCAACCATTTAAAACA+TGG - chr1_4:8266689-8266708 None:intergenic 30.0%
CTATATTGAGGGTCTTAAAT+TGG + chr1_4:8266736-8266755 Msa0148060:CDS 30.0%
CTTAAATTGGCTAATCATGA+TGG + chr1_4:8266749-8266768 Msa0148060:CDS 30.0%
CTGGATTCTTCTATGTTAAG+AGG + chr1_4:8265669-8265688 Msa0148060:CDS 35.0%
CTTTCGAAACTCAAGTTTCT+CGG - chr1_4:8265711-8265730 None:intergenic 35.0%
TCTATCTTCTCTTCGTCATA+TGG + chr1_4:8266422-8266441 Msa0148060:intron 35.0%
TTTCTCCTATATGCATGTGA+AGG + chr1_4:8266526-8266545 Msa0148060:intron 35.0%
GTATATGGTTTGATGTGAGT+TGG + chr1_4:8266548-8266567 Msa0148060:intron 35.0%
CAGATGAATGGTTCAATGAA+AGG + chr1_4:8266625-8266644 Msa0148060:intron 35.0%
TGGTTCAATGAAAGGACTTT+AGG + chr1_4:8266633-8266652 Msa0148060:intron 35.0%
TTCAATGAAAGGACTTTAGG+AGG + chr1_4:8266636-8266655 Msa0148060:intron 35.0%
! TCAATGAAAGGACTTTAGGA+GGG + chr1_4:8266637-8266656 Msa0148060:intron 35.0%
CTAATCATGATGGTCATTCA+GGG + chr1_4:8266759-8266778 Msa0148060:CDS 35.0%
CTGGATTCTTCTATGTTAAG+AGG + chr1_4:8265669-8265688 Msa0148060:CDS 35.0%
CTTTCGAAACTCAAGTTTCT+CGG - chr1_4:8265711-8265730 None:intergenic 35.0%
TCTATCTTCTCTTCGTCATA+TGG + chr1_4:8266422-8266441 Msa0148060:intron 35.0%
TTTCTCCTATATGCATGTGA+AGG + chr1_4:8266526-8266545 Msa0148060:intron 35.0%
GTATATGGTTTGATGTGAGT+TGG + chr1_4:8266548-8266567 Msa0148060:intron 35.0%
CAGATGAATGGTTCAATGAA+AGG + chr1_4:8266625-8266644 Msa0148060:intron 35.0%
TGGTTCAATGAAAGGACTTT+AGG + chr1_4:8266633-8266652 Msa0148060:intron 35.0%
TTCAATGAAAGGACTTTAGG+AGG + chr1_4:8266636-8266655 Msa0148060:intron 35.0%
! TCAATGAAAGGACTTTAGGA+GGG + chr1_4:8266637-8266656 Msa0148060:intron 35.0%
CTAATCATGATGGTCATTCA+GGG + chr1_4:8266759-8266778 Msa0148060:CDS 35.0%
AGTTTCTCGGTAAACTGTCT+TGG - chr1_4:8265698-8265717 None:intergenic 40.0%
AGCACATGACGATTAACTCT+GGG - chr1_4:8265740-8265759 None:intergenic 40.0%
TCGTCATATGGATGAAGAGA+TGG + chr1_4:8266434-8266453 Msa0148060:intron 40.0%
GGTCGAAAATCCATGCAAAT+TGG - chr1_4:8266601-8266620 None:intergenic 40.0%
ATTGAACCATTCATCTGTCG+AGG - chr1_4:8266622-8266641 None:intergenic 40.0%
ATGTAGGGAGCTCTATATTG+AGG + chr1_4:8266724-8266743 Msa0148060:CDS 40.0%
TGTAGGGAGCTCTATATTGA+GGG + chr1_4:8266725-8266744 Msa0148060:CDS 40.0%
GCTAATCATGATGGTCATTC+AGG + chr1_4:8266758-8266777 Msa0148060:CDS 40.0%
ATGGTCATTCAGGGTTTGAA+AGG + chr1_4:8266768-8266787 Msa0148060:CDS 40.0%
AGTTTCTCGGTAAACTGTCT+TGG - chr1_4:8265698-8265717 None:intergenic 40.0%
AGCACATGACGATTAACTCT+GGG - chr1_4:8265740-8265759 None:intergenic 40.0%
TCGTCATATGGATGAAGAGA+TGG + chr1_4:8266434-8266453 Msa0148060:intron 40.0%
GGTCGAAAATCCATGCAAAT+TGG - chr1_4:8266601-8266620 None:intergenic 40.0%
ATTGAACCATTCATCTGTCG+AGG - chr1_4:8266622-8266641 None:intergenic 40.0%
ATGTAGGGAGCTCTATATTG+AGG + chr1_4:8266724-8266743 Msa0148060:CDS 40.0%
TGTAGGGAGCTCTATATTGA+GGG + chr1_4:8266725-8266744 Msa0148060:CDS 40.0%
GCTAATCATGATGGTCATTC+AGG + chr1_4:8266758-8266777 Msa0148060:CDS 40.0%
ATGGTCATTCAGGGTTTGAA+AGG + chr1_4:8266768-8266787 Msa0148060:CDS 40.0%
CAGCACATGACGATTAACTC+TGG - chr1_4:8265741-8265760 None:intergenic 45.0%
! CATAGAAGGCGTGCAACATA+CGG - chr1_4:8266145-8266164 None:intergenic 45.0%
! ATTTGAGGAGGTGCAACAAG+TGG + chr1_4:8266381-8266400 Msa0148060:intron 45.0%
! TTTGAGGAGGTGCAACAAGT+GGG + chr1_4:8266382-8266401 Msa0148060:intron 45.0%
TTTCGACCTCGACAGATGAA+TGG + chr1_4:8266613-8266632 Msa0148060:intron 45.0%
CAGCACATGACGATTAACTC+TGG - chr1_4:8265741-8265760 None:intergenic 45.0%
! CATAGAAGGCGTGCAACATA+CGG - chr1_4:8266145-8266164 None:intergenic 45.0%
! ATTTGAGGAGGTGCAACAAG+TGG + chr1_4:8266381-8266400 Msa0148060:intron 45.0%
! TTTGAGGAGGTGCAACAAGT+GGG + chr1_4:8266382-8266401 Msa0148060:intron 45.0%
TTTCGACCTCGACAGATGAA+TGG + chr1_4:8266613-8266632 Msa0148060:intron 45.0%
!!! TATTTTTAAATTAAAATTTG+AGG + chr1_4:8266366-8266385 Msa0148060:intron 5.0%
!!! TATTTTTAAATTAAAATTTG+AGG + chr1_4:8266366-8266385 Msa0148060:intron 5.0%
CGTGAAACTTGTGTCTGCTG+CGG + chr1_4:8265643-8265662 Msa0148060:CDS 50.0%
GTGAAACTTGTGTCTGCTGC+GGG + chr1_4:8265644-8265663 Msa0148060:CDS 50.0%
TGAAACTTGTGTCTGCTGCG+GGG + chr1_4:8265645-8265664 Msa0148060:CDS 50.0%
! TTGAGGAGGTGCAACAAGTG+GGG + chr1_4:8266383-8266402 Msa0148060:intron 50.0%
! TGAAAGGACTTTAGGAGGGC+TGG + chr1_4:8266641-8266660 Msa0148060:intron 50.0%
CGTGAAACTTGTGTCTGCTG+CGG + chr1_4:8265643-8265662 Msa0148060:CDS 50.0%
GTGAAACTTGTGTCTGCTGC+GGG + chr1_4:8265644-8265663 Msa0148060:CDS 50.0%
TGAAACTTGTGTCTGCTGCG+GGG + chr1_4:8265645-8265664 Msa0148060:CDS 50.0%
! TTGAGGAGGTGCAACAAGTG+GGG + chr1_4:8266383-8266402 Msa0148060:intron 50.0%
! TGAAAGGACTTTAGGAGGGC+TGG + chr1_4:8266641-8266660 Msa0148060:intron 50.0%
!! GACTTTAGGAGGGCTGGTTG+TGG + chr1_4:8266647-8266666 Msa0148060:intron 55.0%
!! TTTAGGAGGGCTGGTTGTGG+TGG + chr1_4:8266650-8266669 Msa0148060:intron 55.0%
!! GACTTTAGGAGGGCTGGTTG+TGG + chr1_4:8266647-8266666 Msa0148060:intron 55.0%
!! TTTAGGAGGGCTGGTTGTGG+TGG + chr1_4:8266650-8266669 Msa0148060:intron 55.0%
CTTGTGTCTGCTGCGGGGAC+TGG + chr1_4:8265650-8265669 Msa0148060:CDS 65.0%
CTTGTGTCTGCTGCGGGGAC+TGG + chr1_4:8265650-8265669 Msa0148060:CDS 65.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 8265611 8266805 8265611 ID=Msa0148060;Name=Msa0148060
chr1_4 mRNA 8265611 8266805 8265611 ID=Msa0148060-mRNA-1;Parent=Msa0148060;Name=Msa0148060-mRNA-1;_AED=0.30;_eAED=0.30;_QI=0|0|0|0.5|1|1|2|0|76
chr1_4 exon 8265611 8265754 8265611 ID=Msa0148060-mRNA-1:exon:3583;Parent=Msa0148060-mRNA-1
chr1_4 exon 8266719 8266805 8266719 ID=Msa0148060-mRNA-1:exon:3584;Parent=Msa0148060-mRNA-1
chr1_4 CDS 8265611 8265754 8265611 ID=Msa0148060-mRNA-1:cds;Parent=Msa0148060-mRNA-1
chr1_4 CDS 8266719 8266805 8266719 ID=Msa0148060-mRNA-1:cds;Parent=Msa0148060-mRNA-1
Gene Sequence

>Msa0148060

ATGGGTAACGCTAAGAAGAAGGCTCAGATGTTCGTGAAACTTGTGTCTGCTGCGGGGACTGGATTCTTCTATGTTAAGAGGAAACCAAGACAGTTTACCGAGAAACTTGAGTTTCGAAAGTATGATCCCAGAGTTAATCGTCATAAGAGATGTAGGGAGCTCTATATTGAGGGTCTTAAATTGGCTAATCATGATGGTCATTCAGGGTTTGAAAGGAGAATTCAATGCTAG

Protein sequence

>Msa0148060

MGNAKKKAQMFVKLVSAAGTGFFYVKRKPRQFTEKLEFRKYDPRVNRHKRCRELYIEGLKLANHDGHSGFERRIQC*