AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0114870

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATATGTTGTCCTAACAATTA+AGG 0.168807 1_3:+33154474 None:intergenic
TCAGTTTACCCTTAATTGTT+AGG 0.300983 1_3:-33154483 Msa0114870:three_prime_UTR
CATGATATGTTAGCCTGTTC+TGG 0.307236 1_3:-33154708 Msa0114870:three_prime_UTR
TAGATCTCTGTCTCATCTTT+CGG 0.343914 1_3:+33154782 None:intergenic
AAACTGAAAGCATAAATAAA+AGG 0.357989 1_3:+33154499 None:intergenic
TATGTTGTCCTAACAATTAA+GGG 0.364409 1_3:+33154475 None:intergenic
TATTGGTGCATGTACTGTTT+TGG 0.389852 1_3:-33154589 Msa0114870:three_prime_UTR
CTTTCCATCTTGCTTGATGC+CGG 0.419505 1_3:-33154734 Msa0114870:three_prime_UTR
ATCTTGAGGTTTGGTTGGGA+GGG 0.425741 1_3:-33154678 Msa0114870:three_prime_UTR
GATCTTGAGGTTTGGTTGGG+AGG 0.451658 1_3:-33154679 Msa0114870:three_prime_UTR
CAAGATCATTACACCAGAAC+AGG 0.453287 1_3:+33154695 None:intergenic
GCAAATTTCCGCGGCAGGGA+AGG 0.455538 1_3:+33155923 None:intergenic
TCTTGAGGTTTGGTTGGGAG+GGG 0.455633 1_3:-33154677 Msa0114870:three_prime_UTR
GGTGTAATGATCTTGAGGTT+TGG 0.457532 1_3:-33154687 Msa0114870:three_prime_UTR
TAATGATCTTGAGGTTTGGT+TGG 0.461100 1_3:-33154683 Msa0114870:three_prime_UTR
CGATGGCAAATTTCCGCGGC+AGG 0.470724 1_3:+33155918 None:intergenic
AATGATCTTGAGGTTTGGTT+GGG 0.481359 1_3:-33154682 Msa0114870:three_prime_UTR
ATGAAAATCATGTGGATGAG+GGG 0.489922 1_3:-33154363 Msa0114870:three_prime_UTR
ACATGAAAATCATGTGGATG+AGG 0.501805 1_3:-33154365 Msa0114870:three_prime_UTR
AGCGTATGAGGCGGTTGAAG+AGG 0.522163 1_3:-33154810 Msa0114870:CDS
GTTCTGGTGTAATGATCTTG+AGG 0.522478 1_3:-33154692 Msa0114870:three_prime_UTR
AATTACCTTGGCTCTCATGT+TGG 0.522645 1_3:+33155862 None:intergenic
GATGAGACAGAGATCTAAGT+AGG 0.529953 1_3:-33154776 Msa0114870:exon
ATCTAAGTAGGTGCATATGC+AGG 0.532333 1_3:-33154764 Msa0114870:exon
TTCAGTTCCCTTCCCTGCCG+CGG 0.533826 1_3:-33155931 Msa0114870:CDS
AGTGGAAGAAGAAGCGTATG+AGG 0.535090 1_3:-33154822 Msa0114870:CDS
CACTGGGTTAGCTTAAGATG+TGG 0.544623 1_3:-33154426 Msa0114870:three_prime_UTR
CATGAAAATCATGTGGATGA+GGG 0.544921 1_3:-33154364 Msa0114870:three_prime_UTR
CAAATTTCCGCGGCAGGGAA+GGG 0.545646 1_3:+33155924 None:intergenic
GAAGCCGGCATCAAGCAAGA+TGG 0.582153 1_3:+33154730 None:intergenic
AGGCTAACATATCATGAAGC+CGG 0.585518 1_3:+33154715 None:intergenic
GTGCTGTGTTTCGTAAGAAG+CGG 0.597751 1_3:+33155989 None:intergenic
ATCTTAAGCTAACCCAGTGA+AGG 0.623767 1_3:+33154430 None:intergenic
TGAAAATCATGTGGATGAGG+GGG 0.624026 1_3:-33154362 Msa0114870:three_prime_UTR
ATCATCCAACATGAGAGCCA+AGG 0.635896 1_3:-33155867 Msa0114870:intron
CTGAAAGCATAAATAAAAGG+AGG 0.640654 1_3:+33154502 None:intergenic
TTAAATACATGAAAATCATG+TGG 0.661283 1_3:-33154371 Msa0114870:three_prime_UTR
GATGGCAAATTTCCGCGGCA+GGG 0.670700 1_3:+33155919 None:intergenic
CACTATAAATACGAAAACGA+GGG 0.686259 1_3:-33155969 Msa0114870:CDS
GCACTATAAATACGAAAACG+AGG 0.689830 1_3:-33155970 Msa0114870:CDS
TCTCAAGTTTCTCTGTACGA+TGG 0.694510 1_3:+33155901 None:intergenic
TCAATTGTCTTAGAACAACA+AGG 0.700588 1_3:+33154629 None:intergenic
GGAAGAAGAAGCGTATGAGG+CGG 0.708289 1_3:-33154819 Msa0114870:CDS
TGTACGATGGCAAATTTCCG+CGG 0.709054 1_3:+33155914 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAAATGTTCAATATTTT+TGG - chr1_3:33154557-33154576 Msa0114870:three_prime_UTR 10.0%
!!! TTTAAATGTTCAATATTTTT+GGG - chr1_3:33154558-33154577 Msa0114870:three_prime_UTR 10.0%
!! AAAAAATATTATTCATTTCT+GGG + chr1_3:33154884-33154903 None:intergenic 10.0%
!!! TTTTAAATGTTCAATATTTT+TGG - chr1_3:33154557-33154576 Msa0114870:three_prime_UTR 10.0%
!!! TTTAAATGTTCAATATTTTT+GGG - chr1_3:33154558-33154577 Msa0114870:three_prime_UTR 10.0%
!! AAAAAATATTATTCATTTCT+GGG + chr1_3:33154884-33154903 None:intergenic 10.0%
!! ATTAGATACAATTATGATTT+AGG - chr1_3:33154581-33154600 Msa0114870:three_prime_UTR 15.0%
!! TTAGATACAATTATGATTTA+GGG - chr1_3:33154582-33154601 Msa0114870:three_prime_UTR 15.0%
!! GAAAAAATATTATTCATTTC+TGG + chr1_3:33154885-33154904 None:intergenic 15.0%
!! TAAATTAAATGTGATTGTAT+TGG - chr1_3:33154988-33155007 Msa0114870:intron 15.0%
!! ATTAGATACAATTATGATTT+AGG - chr1_3:33154581-33154600 Msa0114870:three_prime_UTR 15.0%
!! TTAGATACAATTATGATTTA+GGG - chr1_3:33154582-33154601 Msa0114870:three_prime_UTR 15.0%
!! GAAAAAATATTATTCATTTC+TGG + chr1_3:33154885-33154904 None:intergenic 15.0%
!! TAAATTAAATGTGATTGTAT+TGG - chr1_3:33154988-33155007 Msa0114870:intron 15.0%
!! TTTATATAATCAATCGAAGA+AGG + chr1_3:33154508-33154527 None:intergenic 20.0%
!! AAAACACATCAAACATTAAA+CGG + chr1_3:33154636-33154655 None:intergenic 20.0%
!!! AGTCAATTTGATGATTTTTT+AGG - chr1_3:33154762-33154781 Msa0114870:exon 20.0%
!! ATCACTGTTTAAAAATACTT+GGG + chr1_3:33154910-33154929 None:intergenic 20.0%
!! AATCACTGTTTAAAAATACT+TGG + chr1_3:33154911-33154930 None:intergenic 20.0%
!!! AAACTGAAAGCATAAATAAA+AGG + chr1_3:33155845-33155864 None:intergenic 20.0%
!! TTAAATACATGAAAATCATG+TGG - chr1_3:33155970-33155989 Msa0114870:CDS 20.0%
!! TTTATATAATCAATCGAAGA+AGG + chr1_3:33154508-33154527 None:intergenic 20.0%
!! AAAACACATCAAACATTAAA+CGG + chr1_3:33154636-33154655 None:intergenic 20.0%
!!! AGTCAATTTGATGATTTTTT+AGG - chr1_3:33154762-33154781 Msa0114870:exon 20.0%
!! ATCACTGTTTAAAAATACTT+GGG + chr1_3:33154910-33154929 None:intergenic 20.0%
!! AATCACTGTTTAAAAATACT+TGG + chr1_3:33154911-33154930 None:intergenic 20.0%
!!! AAACTGAAAGCATAAATAAA+AGG + chr1_3:33155845-33155864 None:intergenic 20.0%
!! TTAAATACATGAAAATCATG+TGG - chr1_3:33155970-33155989 Msa0114870:CDS 20.0%
!! TACAATTATGATTTAGGGTT+TGG - chr1_3:33154587-33154606 Msa0114870:three_prime_UTR 25.0%
!! ACAATTATGATTTAGGGTTT+GGG - chr1_3:33154588-33154607 Msa0114870:three_prime_UTR 25.0%
! TTCACATAATCATTTCTATG+AGG + chr1_3:33154679-33154698 None:intergenic 25.0%
! ATAATAGCGGTAATTATCTA+TGG + chr1_3:33155022-33155041 None:intergenic 25.0%
!!! TGAGTTTTAACTGATAATAG+CGG + chr1_3:33155035-33155054 None:intergenic 25.0%
! CTTAAGAAATTACACAATCT+CGG + chr1_3:33155120-33155139 None:intergenic 25.0%
! TATATCATAAGGTTACAACA+TGG + chr1_3:33155189-33155208 None:intergenic 25.0%
! CATACAGAGTATATATCATA+AGG + chr1_3:33155200-33155219 None:intergenic 25.0%
! TATGATATATACTCTGTATG+CGG - chr1_3:33155200-33155219 Msa0114870:intron 25.0%
!!! CATTTTGTGGTTTTTAAGTA+GGG - chr1_3:33155261-33155280 Msa0114870:intron 25.0%
!!! TATGTATTTTTTTCCCATTG+TGG - chr1_3:33155429-33155448 Msa0114870:intron 25.0%
!!! TTTTGAGTGTTTTGTAGTAT+TGG - chr1_3:33155735-33155754 Msa0114870:intron 25.0%
! TATGTTGTCCTAACAATTAA+GGG + chr1_3:33155869-33155888 None:intergenic 25.0%
! ATATGTTGTCCTAACAATTA+AGG + chr1_3:33155870-33155889 None:intergenic 25.0%
!! TACAATTATGATTTAGGGTT+TGG - chr1_3:33154587-33154606 Msa0114870:three_prime_UTR 25.0%
!! ACAATTATGATTTAGGGTTT+GGG - chr1_3:33154588-33154607 Msa0114870:three_prime_UTR 25.0%
! TTCACATAATCATTTCTATG+AGG + chr1_3:33154679-33154698 None:intergenic 25.0%
! ATAATAGCGGTAATTATCTA+TGG + chr1_3:33155022-33155041 None:intergenic 25.0%
!!! TGAGTTTTAACTGATAATAG+CGG + chr1_3:33155035-33155054 None:intergenic 25.0%
! CTTAAGAAATTACACAATCT+CGG + chr1_3:33155120-33155139 None:intergenic 25.0%
! TATATCATAAGGTTACAACA+TGG + chr1_3:33155189-33155208 None:intergenic 25.0%
! CATACAGAGTATATATCATA+AGG + chr1_3:33155200-33155219 None:intergenic 25.0%
! TATGATATATACTCTGTATG+CGG - chr1_3:33155200-33155219 Msa0114870:intron 25.0%
!!! CATTTTGTGGTTTTTAAGTA+GGG - chr1_3:33155261-33155280 Msa0114870:intron 25.0%
!!! TATGTATTTTTTTCCCATTG+TGG - chr1_3:33155429-33155448 Msa0114870:intron 25.0%
!!! TTTTGAGTGTTTTGTAGTAT+TGG - chr1_3:33155735-33155754 Msa0114870:intron 25.0%
! TATGTTGTCCTAACAATTAA+GGG + chr1_3:33155869-33155888 None:intergenic 25.0%
! ATATGTTGTCCTAACAATTA+AGG + chr1_3:33155870-33155889 None:intergenic 25.0%
CACTATAAATACGAAAACGA+GGG - chr1_3:33154372-33154391 Msa0114870:three_prime_UTR 30.0%
! ACAACCATGTTTCTATCATT+TGG + chr1_3:33154719-33154738 None:intergenic 30.0%
ACTGTTTAAAAATACTTGGG+CGG + chr1_3:33154907-33154926 None:intergenic 30.0%
AATTTATGACCAATGAACCT+CGG + chr1_3:33154955-33154974 None:intergenic 30.0%
CAATTCAGTTAATAAGCCAA+AGG + chr1_3:33155084-33155103 None:intergenic 30.0%
CGAGATTGTGTAATTTCTTA+AGG - chr1_3:33155118-33155137 Msa0114870:intron 30.0%
ACTTAAAAACCACAAAATGC+TGG + chr1_3:33155260-33155279 None:intergenic 30.0%
!!! GCATTTTGTGGTTTTTAAGT+AGG - chr1_3:33155260-33155279 Msa0114870:intron 30.0%
! CTTCATAACAGTATTTTGCA+TGG - chr1_3:33155356-33155375 Msa0114870:intron 30.0%
TTCATAACAGTATCATGCTT+TGG - chr1_3:33155396-33155415 Msa0114870:intron 30.0%
TCAATGATGTTATGTTTCAG+TGG - chr1_3:33155501-33155520 Msa0114870:intron 30.0%
TCAATTGTCTTAGAACAACA+AGG + chr1_3:33155715-33155734 None:intergenic 30.0%
! CTGAAAGCATAAATAAAAGG+AGG + chr1_3:33155842-33155861 None:intergenic 30.0%
TCAGTTTACCCTTAATTGTT+AGG - chr1_3:33155858-33155877 Msa0114870:intron 30.0%
! ATTTTATGATATGCCTTCAC+TGG - chr1_3:33155898-33155917 Msa0114870:CDS 30.0%
! TTTTATGATATGCCTTCACT+GGG - chr1_3:33155899-33155918 Msa0114870:CDS 30.0%
CACTATAAATACGAAAACGA+GGG - chr1_3:33154372-33154391 Msa0114870:three_prime_UTR 30.0%
! ACAACCATGTTTCTATCATT+TGG + chr1_3:33154719-33154738 None:intergenic 30.0%
ACTGTTTAAAAATACTTGGG+CGG + chr1_3:33154907-33154926 None:intergenic 30.0%
AATTTATGACCAATGAACCT+CGG + chr1_3:33154955-33154974 None:intergenic 30.0%
CAATTCAGTTAATAAGCCAA+AGG + chr1_3:33155084-33155103 None:intergenic 30.0%
CGAGATTGTGTAATTTCTTA+AGG - chr1_3:33155118-33155137 Msa0114870:intron 30.0%
ACTTAAAAACCACAAAATGC+TGG + chr1_3:33155260-33155279 None:intergenic 30.0%
!!! GCATTTTGTGGTTTTTAAGT+AGG - chr1_3:33155260-33155279 Msa0114870:intron 30.0%
! CTTCATAACAGTATTTTGCA+TGG - chr1_3:33155356-33155375 Msa0114870:intron 30.0%
TTCATAACAGTATCATGCTT+TGG - chr1_3:33155396-33155415 Msa0114870:intron 30.0%
TCAATGATGTTATGTTTCAG+TGG - chr1_3:33155501-33155520 Msa0114870:intron 30.0%
TCAATTGTCTTAGAACAACA+AGG + chr1_3:33155715-33155734 None:intergenic 30.0%
! CTGAAAGCATAAATAAAAGG+AGG + chr1_3:33155842-33155861 None:intergenic 30.0%
TCAGTTTACCCTTAATTGTT+AGG - chr1_3:33155858-33155877 Msa0114870:intron 30.0%
! ATTTTATGATATGCCTTCAC+TGG - chr1_3:33155898-33155917 Msa0114870:CDS 30.0%
! TTTTATGATATGCCTTCACT+GGG - chr1_3:33155899-33155918 Msa0114870:CDS 30.0%
GCACTATAAATACGAAAACG+AGG - chr1_3:33154371-33154390 Msa0114870:three_prime_UTR 35.0%
! ATGATTTAGGGTTTGGGTTT+AGG - chr1_3:33154594-33154613 Msa0114870:three_prime_UTR 35.0%
CGTTCCAAATGATAGAAACA+TGG - chr1_3:33154712-33154731 Msa0114870:three_prime_UTR 35.0%
TACTATAATCCGAGGTTCAT+TGG - chr1_3:33154943-33154962 Msa0114870:intron 35.0%
!!! TTTTTTCCCATTGTGGTTGT+TGG - chr1_3:33155436-33155455 Msa0114870:intron 35.0%
TAGATCTCTGTCTCATCTTT+CGG + chr1_3:33155562-33155581 None:intergenic 35.0%
! TAATGATCTTGAGGTTTGGT+TGG - chr1_3:33155658-33155677 Msa0114870:intron 35.0%
! AATGATCTTGAGGTTTGGTT+GGG - chr1_3:33155659-33155678 Msa0114870:intron 35.0%
! TATTGGTGCATGTACTGTTT+TGG - chr1_3:33155752-33155771 Msa0114870:intron 35.0%
ACATGAAAATCATGTGGATG+AGG - chr1_3:33155976-33155995 Msa0114870:CDS 35.0%
CATGAAAATCATGTGGATGA+GGG - chr1_3:33155977-33155996 Msa0114870:CDS 35.0%
ATGAAAATCATGTGGATGAG+GGG - chr1_3:33155978-33155997 Msa0114870:CDS 35.0%
GCACTATAAATACGAAAACG+AGG - chr1_3:33154371-33154390 Msa0114870:three_prime_UTR 35.0%
! ATGATTTAGGGTTTGGGTTT+AGG - chr1_3:33154594-33154613 Msa0114870:three_prime_UTR 35.0%
CGTTCCAAATGATAGAAACA+TGG - chr1_3:33154712-33154731 Msa0114870:three_prime_UTR 35.0%
TACTATAATCCGAGGTTCAT+TGG - chr1_3:33154943-33154962 Msa0114870:intron 35.0%
!!! TTTTTTCCCATTGTGGTTGT+TGG - chr1_3:33155436-33155455 Msa0114870:intron 35.0%
TAGATCTCTGTCTCATCTTT+CGG + chr1_3:33155562-33155581 None:intergenic 35.0%
! TAATGATCTTGAGGTTTGGT+TGG - chr1_3:33155658-33155677 Msa0114870:intron 35.0%
! AATGATCTTGAGGTTTGGTT+GGG - chr1_3:33155659-33155678 Msa0114870:intron 35.0%
! TATTGGTGCATGTACTGTTT+TGG - chr1_3:33155752-33155771 Msa0114870:intron 35.0%
ACATGAAAATCATGTGGATG+AGG - chr1_3:33155976-33155995 Msa0114870:CDS 35.0%
CATGAAAATCATGTGGATGA+GGG - chr1_3:33155977-33155996 Msa0114870:CDS 35.0%
ATGAAAATCATGTGGATGAG+GGG - chr1_3:33155978-33155997 Msa0114870:CDS 35.0%
TCTCAAGTTTCTCTGTACGA+TGG + chr1_3:33154443-33154462 None:intergenic 40.0%
! AATTACCTTGGCTCTCATGT+TGG + chr1_3:33154482-33154501 None:intergenic 40.0%
CGAAGAAGGATGAATTACCT+TGG + chr1_3:33154494-33154513 None:intergenic 40.0%
TGACAGTGTACTATAATCCG+AGG - chr1_3:33154935-33154954 Msa0114870:intron 40.0%
ACACAATCTCGGCATCTTAA+TGG + chr1_3:33155109-33155128 None:intergenic 40.0%
GATATATACTCTGTATGCGG+AGG - chr1_3:33155203-33155222 Msa0114870:intron 40.0%
!!! TGGTTTTTAAGTAGGGCTAG+TGG - chr1_3:33155268-33155287 Msa0114870:intron 40.0%
TGTCAATGACTACCGTAGAA+TGG + chr1_3:33155333-33155352 None:intergenic 40.0%
ATCAAGCCAACAACCACAAT+GGG + chr1_3:33155445-33155464 None:intergenic 40.0%
AATCAAGCCAACAACCACAA+TGG + chr1_3:33155446-33155465 None:intergenic 40.0%
GATGAGACAGAGATCTAAGT+AGG - chr1_3:33155565-33155584 Msa0114870:intron 40.0%
ATCTAAGTAGGTGCATATGC+AGG - chr1_3:33155577-33155596 Msa0114870:intron 40.0%
AGGCTAACATATCATGAAGC+CGG + chr1_3:33155629-33155648 None:intergenic 40.0%
! CATGATATGTTAGCCTGTTC+TGG - chr1_3:33155633-33155652 Msa0114870:intron 40.0%
CAAGATCATTACACCAGAAC+AGG + chr1_3:33155649-33155668 None:intergenic 40.0%
GTTCTGGTGTAATGATCTTG+AGG - chr1_3:33155649-33155668 Msa0114870:intron 40.0%
GGTGTAATGATCTTGAGGTT+TGG - chr1_3:33155654-33155673 Msa0114870:intron 40.0%
ATCTTAAGCTAACCCAGTGA+AGG + chr1_3:33155914-33155933 None:intergenic 40.0%
TGAAAATCATGTGGATGAGG+GGG - chr1_3:33155979-33155998 Msa0114870:CDS 40.0%
TCTCAAGTTTCTCTGTACGA+TGG + chr1_3:33154443-33154462 None:intergenic 40.0%
! AATTACCTTGGCTCTCATGT+TGG + chr1_3:33154482-33154501 None:intergenic 40.0%
CGAAGAAGGATGAATTACCT+TGG + chr1_3:33154494-33154513 None:intergenic 40.0%
TGACAGTGTACTATAATCCG+AGG - chr1_3:33154935-33154954 Msa0114870:intron 40.0%
ACACAATCTCGGCATCTTAA+TGG + chr1_3:33155109-33155128 None:intergenic 40.0%
GATATATACTCTGTATGCGG+AGG - chr1_3:33155203-33155222 Msa0114870:intron 40.0%
!!! TGGTTTTTAAGTAGGGCTAG+TGG - chr1_3:33155268-33155287 Msa0114870:intron 40.0%
TGTCAATGACTACCGTAGAA+TGG + chr1_3:33155333-33155352 None:intergenic 40.0%
ATCAAGCCAACAACCACAAT+GGG + chr1_3:33155445-33155464 None:intergenic 40.0%
AATCAAGCCAACAACCACAA+TGG + chr1_3:33155446-33155465 None:intergenic 40.0%
GATGAGACAGAGATCTAAGT+AGG - chr1_3:33155565-33155584 Msa0114870:intron 40.0%
ATCTAAGTAGGTGCATATGC+AGG - chr1_3:33155577-33155596 Msa0114870:intron 40.0%
AGGCTAACATATCATGAAGC+CGG + chr1_3:33155629-33155648 None:intergenic 40.0%
! CATGATATGTTAGCCTGTTC+TGG - chr1_3:33155633-33155652 Msa0114870:intron 40.0%
CAAGATCATTACACCAGAAC+AGG + chr1_3:33155649-33155668 None:intergenic 40.0%
GTTCTGGTGTAATGATCTTG+AGG - chr1_3:33155649-33155668 Msa0114870:intron 40.0%
GGTGTAATGATCTTGAGGTT+TGG - chr1_3:33155654-33155673 Msa0114870:intron 40.0%
ATCTTAAGCTAACCCAGTGA+AGG + chr1_3:33155914-33155933 None:intergenic 40.0%
TGAAAATCATGTGGATGAGG+GGG - chr1_3:33155979-33155998 Msa0114870:CDS 40.0%
GTGCTGTGTTTCGTAAGAAG+CGG + chr1_3:33154355-33154374 None:intergenic 45.0%
TGTACGATGGCAAATTTCCG+CGG + chr1_3:33154430-33154449 None:intergenic 45.0%
ATCATCCAACATGAGAGCCA+AGG - chr1_3:33154474-33154493 Msa0114870:three_prime_UTR 45.0%
! GTCTTTGAGCCAGCATTTTG+TGG - chr1_3:33155248-33155267 Msa0114870:intron 45.0%
CCTTCCTGATAGCCATTCTA+CGG - chr1_3:33155318-33155337 Msa0114870:intron 45.0%
ACTACCGTAGAATGGCTATC+AGG + chr1_3:33155325-33155344 None:intergenic 45.0%
! AGTGGAAGAAGAAGCGTATG+AGG - chr1_3:33155519-33155538 Msa0114870:intron 45.0%
CTTTCCATCTTGCTTGATGC+CGG - chr1_3:33155607-33155626 Msa0114870:intron 45.0%
! ATCTTGAGGTTTGGTTGGGA+GGG - chr1_3:33155663-33155682 Msa0114870:intron 45.0%
!! TGGTGCATGTACTGTTTTGG+TGG - chr1_3:33155755-33155774 Msa0114870:intron 45.0%
!! CACTGGGTTAGCTTAAGATG+TGG - chr1_3:33155915-33155934 Msa0114870:CDS 45.0%
GTGCTGTGTTTCGTAAGAAG+CGG + chr1_3:33154355-33154374 None:intergenic 45.0%
TGTACGATGGCAAATTTCCG+CGG + chr1_3:33154430-33154449 None:intergenic 45.0%
ATCATCCAACATGAGAGCCA+AGG - chr1_3:33154474-33154493 Msa0114870:three_prime_UTR 45.0%
! GTCTTTGAGCCAGCATTTTG+TGG - chr1_3:33155248-33155267 Msa0114870:intron 45.0%
CCTTCCTGATAGCCATTCTA+CGG - chr1_3:33155318-33155337 Msa0114870:intron 45.0%
ACTACCGTAGAATGGCTATC+AGG + chr1_3:33155325-33155344 None:intergenic 45.0%
! AGTGGAAGAAGAAGCGTATG+AGG - chr1_3:33155519-33155538 Msa0114870:intron 45.0%
CTTTCCATCTTGCTTGATGC+CGG - chr1_3:33155607-33155626 Msa0114870:intron 45.0%
! ATCTTGAGGTTTGGTTGGGA+GGG - chr1_3:33155663-33155682 Msa0114870:intron 45.0%
!! TGGTGCATGTACTGTTTTGG+TGG - chr1_3:33155755-33155774 Msa0114870:intron 45.0%
!! CACTGGGTTAGCTTAAGATG+TGG - chr1_3:33155915-33155934 Msa0114870:CDS 45.0%
! ATAAGCCAAAGGCGCAATGC+TGG + chr1_3:33155073-33155092 None:intergenic 50.0%
CCGTAGAATGGCTATCAGGA+AGG + chr1_3:33155321-33155340 None:intergenic 50.0%
! GGAAGAAGAAGCGTATGAGG+CGG - chr1_3:33155522-33155541 Msa0114870:intron 50.0%
! GATCTTGAGGTTTGGTTGGG+AGG - chr1_3:33155662-33155681 Msa0114870:intron 50.0%
! TCTTGAGGTTTGGTTGGGAG+GGG - chr1_3:33155664-33155683 Msa0114870:intron 50.0%
GAGGGGGCAAAAATGCTTTC+AGG - chr1_3:33155995-33156014 Msa0114870:CDS 50.0%
! ATAAGCCAAAGGCGCAATGC+TGG + chr1_3:33155073-33155092 None:intergenic 50.0%
CCGTAGAATGGCTATCAGGA+AGG + chr1_3:33155321-33155340 None:intergenic 50.0%
! GGAAGAAGAAGCGTATGAGG+CGG - chr1_3:33155522-33155541 Msa0114870:intron 50.0%
! GATCTTGAGGTTTGGTTGGG+AGG - chr1_3:33155662-33155681 Msa0114870:intron 50.0%
! TCTTGAGGTTTGGTTGGGAG+GGG - chr1_3:33155664-33155683 Msa0114870:intron 50.0%
CAAATTTCCGCGGCAGGGAA+GGG + chr1_3:33154420-33154439 None:intergenic 55.0%
GATGGCAAATTTCCGCGGCA+GGG + chr1_3:33154425-33154444 None:intergenic 55.0%
TGCTACCAGCATTGCGCCTT+TGG - chr1_3:33155065-33155084 Msa0114870:intron 55.0%
AGCGTATGAGGCGGTTGAAG+AGG - chr1_3:33155531-33155550 Msa0114870:intron 55.0%
GAAGCCGGCATCAAGCAAGA+TGG + chr1_3:33155614-33155633 None:intergenic 55.0%
CAAATTTCCGCGGCAGGGAA+GGG + chr1_3:33154420-33154439 None:intergenic 55.0%
GATGGCAAATTTCCGCGGCA+GGG + chr1_3:33154425-33154444 None:intergenic 55.0%
TGCTACCAGCATTGCGCCTT+TGG - chr1_3:33155065-33155084 Msa0114870:intron 55.0%
AGCGTATGAGGCGGTTGAAG+AGG - chr1_3:33155531-33155550 Msa0114870:intron 55.0%
GAAGCCGGCATCAAGCAAGA+TGG + chr1_3:33155614-33155633 None:intergenic 55.0%
TTCAGTTCCCTTCCCTGCCG+CGG - chr1_3:33154410-33154429 Msa0114870:three_prime_UTR 60.0%
GCAAATTTCCGCGGCAGGGA+AGG + chr1_3:33154421-33154440 None:intergenic 60.0%
CGATGGCAAATTTCCGCGGC+AGG + chr1_3:33154426-33154445 None:intergenic 60.0%
TTCAGTTCCCTTCCCTGCCG+CGG - chr1_3:33154410-33154429 Msa0114870:three_prime_UTR 60.0%
GCAAATTTCCGCGGCAGGGA+AGG + chr1_3:33154421-33154440 None:intergenic 60.0%
CGATGGCAAATTTCCGCGGC+AGG + chr1_3:33154426-33154445 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 33154348 33156015 33154348 ID=Msa0114870;Name=Msa0114870
chr1_3 mRNA 33154348 33156015 33154348 ID=Msa0114870-mRNA-1;Parent=Msa0114870;Name=Msa0114870-mRNA-1;_AED=0.02;_eAED=0.02;_QI=0|1|0.5|1|1|1|2|433|69
chr1_3 exon 33154348 33154842 33154348 ID=Msa0114870-mRNA-1:exon:10453;Parent=Msa0114870-mRNA-1
chr1_3 exon 33155868 33156015 33155868 ID=Msa0114870-mRNA-1:exon:10452;Parent=Msa0114870-mRNA-1
chr1_3 CDS 33155868 33156015 33155868 ID=Msa0114870-mRNA-1:cds;Parent=Msa0114870-mRNA-1
chr1_3 CDS 33154781 33154842 33154781 ID=Msa0114870-mRNA-1:cds;Parent=Msa0114870-mRNA-1
chr1_3 three_prime_UTR 33154348 33154780 33154348 ID=Msa0114870-mRNA-1:three_prime_utr;Parent=Msa0114870-mRNA-1
Gene Sequence

>Msa0114870

GGCTCCGCTTCTTACGAAACACAGCACTATAAATACGAAAACGAGGGTTTTTCACTCTCAGATTCAGTTCCCTTCCCTGCCGCGGAAATTTGCCATCGTACAGAGAAACTTGAGAGCGTAGCGTTCATCATCCAACATGAGAGCCAAGTGGAAGAAGAAGCGTATGAGGCGGTTGAAGAGGAAGCGCCGAAAGATGAGACAGAGATCTAA
Protein sequence

>Msa0114870

GSASYETQHYKYENEGFSLSDSVPFPAAEICHRTEKLESVAFIIQHESQVEEEAYEAVEEEAPKDETEI*