AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0605600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605600 MtrunA17_Chr4g0034961 97.619 84 2 0 1 84 1 84 1.00e-53 161
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0605600 AT4G39300.1 50.000 94 38 2 4 88 3 96 1.81e-22 83.6
Msa0605600 AT4G39300.2 50.000 90 36 2 4 84 3 92 5.96e-21 79.7

Find 61 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTGTCACATTACATTATT+TGG 0.054919 4_2:-51536992 Msa0605600:three_prime_UTR
TAACTTCACAGTTGAAATTT+TGG 0.183736 4_2:-51537420 Msa0605600:three_prime_UTR
GTTGGTTAACATGTTAAAAT+TGG 0.204971 4_2:-51537029 Msa0605600:three_prime_UTR
CCCAGCAAGGATTGAAATTA+TGG 0.210912 4_2:+51537070 None:intergenic
CATATTCTTTCAAGAAAATA+AGG 0.255328 4_2:+51537355 None:intergenic
GTGACAGCCATAAGGAAAAT+AGG 0.303563 4_2:+51537006 None:intergenic
TCGCTTCCAATTCCACTTGC+TGG 0.335202 4_2:-51538972 Msa0605600:CDS
AAACATTGTGACAGGCTTGC+TGG 0.342358 4_2:-51537529 Msa0605600:exon
TCCATAATTTCAATCCTTGC+TGG 0.347997 4_2:-51537071 Msa0605600:three_prime_UTR
CATGCTCCAGCAAGTGGAAT+TGG 0.351595 4_2:+51538966 None:intergenic
TTGTTGGTGTTTATAATTGA+TGG 0.354784 4_2:-51537221 Msa0605600:three_prime_UTR
CTCAAGCTCCTCGTGTTTCA+AGG 0.362192 4_2:-51539151 Msa0605600:intron
CTTGTTAGAACTTAGACTTC+AGG 0.389833 4_2:+51537296 None:intergenic
CAGCAAGAGGAAGTTAAATC+TGG 0.401083 4_2:+51537163 None:intergenic
TTCGAATCAGTAACCTTCTT+CGG 0.405127 4_2:-51539282 Msa0605600:five_prime_UTR
GTAAATCTTAAGTTCCTTGT+TGG 0.410016 4_2:-51537237 Msa0605600:three_prime_UTR
CCAGAATTCAAAAGACTAAG+AGG 0.452287 4_2:+51536831 None:intergenic
AGAACGATTCGCGAAGGAAG+TGG 0.453105 4_2:+51539217 None:intergenic
ACTTCAGGACTGAAAATTTG+AGG 0.454803 4_2:+51537311 None:intergenic
GCTTGAAAACAATGCCTAGT+AGG 0.465036 4_2:-51536798 Msa0605600:three_prime_UTR
TCTGAGTAACTTATGACATC+CGG 0.465209 4_2:+51536761 None:intergenic
TACTAGACTTTGCAGTTCCC+CGG 0.470825 4_2:-51537112 Msa0605600:three_prime_UTR
CTTCAGGACTGAAAATTTGA+GGG 0.478290 4_2:+51537312 None:intergenic
CCATAATTTCAATCCTTGCT+GGG 0.478918 4_2:-51537070 Msa0605600:three_prime_UTR
TCAGTAACCTTCTTCGGCAA+TGG 0.504557 4_2:-51539276 Msa0605600:exon
TTGCATTATAGAGTTAATGT+TGG 0.508111 4_2:-51537047 Msa0605600:three_prime_UTR
AATGTAATGTGACAGCCATA+AGG 0.514270 4_2:+51536998 None:intergenic
CTGAGTAACTTATGACATCC+GGG 0.518902 4_2:+51536762 None:intergenic
AATTCTTTAGTCTTTGTCAC+CGG 0.521403 4_2:+51537093 None:intergenic
CTCTATAATGCAACCCAGCA+AGG 0.539629 4_2:+51537057 None:intergenic
GAATCATACAAGTTTACAGT+TGG 0.541756 4_2:+51537382 None:intergenic
AGAAGAAAACAGAAGCGGAA+TGG 0.542095 4_2:+51539172 None:intergenic
CTGTAAATCAGCGGCAAGAG+TGG 0.546457 4_2:-51539243 Msa0605600:CDS
TGAAAGCATGCTCCAGCAAG+TGG 0.547575 4_2:+51538960 None:intergenic
GAAGAAGCCATTGCCGAAGA+AGG 0.549261 4_2:+51539269 None:intergenic
GACTAAGAGGTGAAACTGAA+AGG 0.549510 4_2:+51536844 None:intergenic
GGTTAAACAAGGTTCCTACT+AGG 0.551356 4_2:+51536784 None:intergenic
AGGGTGATTATCAAGCAAGT+TGG 0.561708 4_2:+51537331 None:intergenic
GAAAACAGAAGCGGAATGGA+AGG 0.567367 4_2:+51539176 None:intergenic
GAGTCTGTGATACTGAATGA+TGG 0.573407 4_2:+51536965 None:intergenic
ATTCTTTAGTCTTTGTCACC+GGG 0.589704 4_2:+51537094 None:intergenic
TCTTGCCGCTGATTTACAGA+AGG 0.600725 4_2:+51539247 None:intergenic
TTCTTCCTTCTGTAAATCAG+CGG 0.601690 4_2:-51539252 Msa0605600:CDS
ATGACATCCGGGGTTAAACA+AGG 0.601957 4_2:+51536773 None:intergenic
TGCTGGAGCATGCTTTCACA+AGG 0.601960 4_2:-51538955 Msa0605600:intron
GTAGGAACCTTGTTTAACCC+CGG 0.605549 4_2:-51536780 Msa0605600:three_prime_UTR
AGTCTGTGATACTGAATGAT+GGG 0.609722 4_2:+51536966 None:intergenic
ATGTTCAGAAAATTGATGAG+AGG 0.611908 4_2:+51536908 None:intergenic
AGTGAGTCTAGCATCAGCAA+TGG 0.614389 4_2:+51539002 None:intergenic
GGATCTATTCAAGATGAATG+AGG 0.615866 4_2:-51537508 Msa0605600:three_prime_UTR
CAAGAGGAAGTTAAATCTGG+AGG 0.626990 4_2:+51537166 None:intergenic
TCAGCAATGGCAGTATGAAG+AGG 0.642069 4_2:+51539015 None:intergenic
TATGGATTACAATCAGCAAG+AGG 0.643407 4_2:+51537150 None:intergenic
GCTTGAGAAGAAAACAGAAG+CGG 0.651968 4_2:+51539167 None:intergenic
GGTTCTAGAACGATTCGCGA+AGG 0.656504 4_2:+51539211 None:intergenic
GGGTTCAAGCTAGGATCTAG+CGG 0.662054 4_2:-51539314 None:intergenic
ATCAATTATAAACACCAACA+AGG 0.671646 4_2:+51537223 None:intergenic
TTCTTTAGTCTTTGTCACCG+GGG 0.692354 4_2:+51537095 None:intergenic
TTGTTCTACCTTGAAACACG+AGG 0.698940 4_2:+51539143 None:intergenic
GGATGTCATAAGTTACTCAG+AGG 0.708787 4_2:-51536759 None:intergenic
TGAGTAACTTATGACATCCG+GGG 0.708888 4_2:+51536763 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTAATTTAAAACATAATAT+GGG - chr4_2:51538250-51538269 Msa0605600:intron 10.0%
!!! TTATTTTTGAAGGATTTTAT+CGG - chr4_2:51537005-51537024 Msa0605600:three_prime_UTR 15.0%
!!! TATATAGAAAAAGTTTTCAA+GGG + chr4_2:51537341-51537360 None:intergenic 15.0%
!!! ATATATAGAAAAAGTTTTCA+AGG + chr4_2:51537342-51537361 None:intergenic 15.0%
!!! TTGAAAACTTTTTCTATATA+TGG - chr4_2:51537341-51537360 Msa0605600:three_prime_UTR 15.0%
!!! ATTTTCAAAATCCATTATTT+TGG - chr4_2:51537590-51537609 Msa0605600:intron 15.0%
!!! TTAAATTTTCATAACAAAGA+TGG + chr4_2:51537760-51537779 None:intergenic 15.0%
!!! CCATATTATGTTTTAAATTA+AGG + chr4_2:51538252-51538271 None:intergenic 15.0%
!!! CCTTAATTTAAAACATAATA+TGG - chr4_2:51538249-51538268 Msa0605600:intron 15.0%
!!! AATAACGTGTTTATTTTTGA+AGG - chr4_2:51536995-51537014 Msa0605600:three_prime_UTR 20.0%
!! AGTATGATATATAAGGTTTA+TGG - chr4_2:51537208-51537227 Msa0605600:three_prime_UTR 20.0%
!! AAAGATTATGTATTGTGTTA+TGG - chr4_2:51537257-51537276 Msa0605600:three_prime_UTR 20.0%
!! ATGAATATATGATGAGTTAA+AGG - chr4_2:51537492-51537511 Msa0605600:three_prime_UTR 20.0%
!!! CATATAAACTTTTAGGATTT+GGG + chr4_2:51537979-51537998 None:intergenic 20.0%
!! CAAATCCTAAAAGTTTATAT+GGG - chr4_2:51537978-51537997 Msa0605600:intron 20.0%
!!! AATTTCAATCTTTTTCAACA+AGG - chr4_2:51538015-51538034 Msa0605600:intron 20.0%
!! GTTGATCATTATAAAATGTA+GGG - chr4_2:51538331-51538350 Msa0605600:intron 20.0%
!!! ATCTAGGTTATTTTATTTAC+TGG - chr4_2:51538362-51538381 Msa0605600:intron 20.0%
!! ATATTATATTACTGCAATAG+AGG + chr4_2:51538420-51538439 None:intergenic 20.0%
!! GTAATTACTAATTACCATAT+CGG + chr4_2:51538460-51538479 None:intergenic 20.0%
!! CATATTCTTTCAAGAAAATA+AGG + chr4_2:51538717-51538736 None:intergenic 20.0%
! TTCATGCAGTATGATATATA+AGG - chr4_2:51537201-51537220 Msa0605600:three_prime_UTR 25.0%
! ATATATGATGAGTTAAAGGA+TGG - chr4_2:51537496-51537515 Msa0605600:three_prime_UTR 25.0%
!!! TTTTATTGAGTTGTCACTTT+TGG + chr4_2:51537862-51537881 None:intergenic 25.0%
! AAGTGACAACTCAATAAAAA+TGG - chr4_2:51537863-51537882 Msa0605600:intron 25.0%
!!! CCATATAAACTTTTAGGATT+TGG + chr4_2:51537980-51537999 None:intergenic 25.0%
! CCAAATCCTAAAAGTTTATA+TGG - chr4_2:51537977-51537996 Msa0605600:intron 25.0%
!!! TTTTTCAACAAGGTTGTTTT+GGG - chr4_2:51538025-51538044 Msa0605600:intron 25.0%
! ATTAAGGTTGCTATACAATT+TGG + chr4_2:51538236-51538255 None:intergenic 25.0%
! TCAACAAATAATTGGAATGT+AGG + chr4_2:51538310-51538329 None:intergenic 25.0%
! CGTTGATCATTATAAAATGT+AGG - chr4_2:51538330-51538349 Msa0605600:intron 25.0%
! AATATAATATCGCTTTCAGA+TGG - chr4_2:51538430-51538449 Msa0605600:intron 25.0%
! ATATAATATCGCTTTCAGAT+GGG - chr4_2:51538431-51538450 Msa0605600:intron 25.0%
! ACAAAGTAAAAGAAGATATC+AGG + chr4_2:51538504-51538523 None:intergenic 25.0%
!!! CTTCTTTTACTTTGTTGTAT+AGG - chr4_2:51538509-51538528 Msa0605600:intron 25.0%
!! TAACTTCACAGTTGAAATTT+TGG - chr4_2:51538649-51538668 Msa0605600:intron 25.0%
! ATCAATTATAAACACCAACA+AGG + chr4_2:51538849-51538868 None:intergenic 25.0%
!! TTGTTGGTGTTTATAATTGA+TGG - chr4_2:51538848-51538867 Msa0605600:intron 25.0%
!! TTGTAATCCATATTTTTCGT+TGG - chr4_2:51538930-51538949 Msa0605600:intron 25.0%
! TTGCATTATAGAGTTAATGT+TGG - chr4_2:51539022-51539041 Msa0605600:CDS 25.0%
! GTTGGTTAACATGTTAAAAT+TGG - chr4_2:51539040-51539059 Msa0605600:CDS 25.0%
! CATGGAAATGAATTTTGAAC+TGG - chr4_2:51536968-51536987 Msa0605600:three_prime_UTR 30.0%
!! ACAAGGTACTGATTCTTATT+AGG - chr4_2:51537131-51537150 Msa0605600:three_prime_UTR 30.0%
GTTTCAGTAATTGATTGTCT+AGG - chr4_2:51537282-51537301 Msa0605600:three_prime_UTR 30.0%
ATTAGAGATTAGAGATGAGA+GGG - chr4_2:51537412-51537431 Msa0605600:three_prime_UTR 30.0%
GGTGTAGAATTAAGATAAAG+AGG - chr4_2:51537517-51537536 Msa0605600:three_prime_UTR 30.0%
ATCAGCACTAACCAAAATAA+TGG + chr4_2:51537604-51537623 None:intergenic 30.0%
CATTCATTAGCATTCTTACA+GGG - chr4_2:51537705-51537724 Msa0605600:intron 30.0%
!! TGGCAATCTCAAAGTTTTAT+AGG + chr4_2:51537740-51537759 None:intergenic 30.0%
CAACTCAATAAAAATGGATG+TGG - chr4_2:51537869-51537888 Msa0605600:intron 30.0%
ACAAAGGATAATACCATTGA+GGG + chr4_2:51537935-51537954 None:intergenic 30.0%
AACAAAGGATAATACCATTG+AGG + chr4_2:51537936-51537955 None:intergenic 30.0%
CAATGGTATTATCCTTTGTT+TGG - chr4_2:51537936-51537955 Msa0605600:intron 30.0%
! TGTCTCCCATATAAACTTTT+AGG + chr4_2:51537986-51538005 None:intergenic 30.0%
!!! CTTTTTCAACAAGGTTGTTT+TGG - chr4_2:51538024-51538043 Msa0605600:intron 30.0%
!! ATGGGTTATTTGTTACTTTG+AGG - chr4_2:51538148-51538167 Msa0605600:intron 30.0%
! AAAAGTGCGTTTATACATGT+TGG + chr4_2:51538175-51538194 None:intergenic 30.0%
GATCAACGTCAACAAATAAT+TGG + chr4_2:51538318-51538337 None:intergenic 30.0%
TGTGAACACTGAAAATACTA+AGG - chr4_2:51538385-51538404 Msa0605600:intron 30.0%
ATTACTGCAATAGAGGAATT+CGG + chr4_2:51538413-51538432 None:intergenic 30.0%
GATGAGCATAAAGAAGAAAA+AGG + chr4_2:51538482-51538501 None:intergenic 30.0%
ACTTGAAAAAACATTGTGAC+AGG - chr4_2:51538532-51538551 Msa0605600:intron 30.0%
!!! TGAAGTTATTTTTCCTCCAA+GGG + chr4_2:51538637-51538656 None:intergenic 30.0%
GAATCATACAAGTTTACAGT+TGG + chr4_2:51538690-51538709 None:intergenic 30.0%
GTAAATCTTAAGTTCCTTGT+TGG - chr4_2:51538832-51538851 Msa0605600:intron 30.0%
GTACAAACCAACGAAAAATA+TGG + chr4_2:51538940-51538959 None:intergenic 30.0%
! AATTCTTTAGTCTTTGTCAC+CGG + chr4_2:51538979-51538998 None:intergenic 30.0%
!! AAATTGGCCTATTTTCCTTA+TGG - chr4_2:51539056-51539075 Msa0605600:intron 30.0%
ATGTTCAGAAAATTGATGAG+AGG + chr4_2:51539164-51539183 None:intergenic 30.0%
TTCGAATCAGTAACCTTCTT+CGG - chr4_2:51536787-51536806 Msa0605600:three_prime_UTR 35.0%
TTCTTCCTTCTGTAAATCAG+CGG - chr4_2:51536817-51536836 Msa0605600:three_prime_UTR 35.0%
!! TGAAGGATTTTATCGGTTGT+TGG - chr4_2:51537012-51537031 Msa0605600:three_prime_UTR 35.0%
GATTAGAGATTAGAGATGAG+AGG - chr4_2:51537411-51537430 Msa0605600:three_prime_UTR 35.0%
TTAGAGATTAGAGATGAGAG+GGG - chr4_2:51537413-51537432 Msa0605600:three_prime_UTR 35.0%
! AGATGGAATAGATTTTGTGG+TGG + chr4_2:51537645-51537664 None:intergenic 35.0%
! TCGAGATGGAATAGATTTTG+TGG + chr4_2:51537648-51537667 None:intergenic 35.0%
! GCTAGAAATTAGTTTCGAGA+TGG + chr4_2:51537662-51537681 None:intergenic 35.0%
CAATGAAGCAATATTATCCG+CGG + chr4_2:51537684-51537703 None:intergenic 35.0%
GCATTCATTAGCATTCTTAC+AGG - chr4_2:51537704-51537723 Msa0605600:intron 35.0%
AAGTGTGTGTCTGTACTAAT+TGG - chr4_2:51537833-51537852 Msa0605600:intron 35.0%
! ATCCTTTGTTTGGAGAGATT+TGG - chr4_2:51537946-51537965 Msa0605600:intron 35.0%
! TCCTTTGTTTGGAGAGATTT+GGG - chr4_2:51537947-51537966 Msa0605600:intron 35.0%
ATTAAGACCAGCTTGATGAA+TGG - chr4_2:51538129-51538148 Msa0605600:intron 35.0%
TTAAGACCAGCTTGATGAAT+GGG - chr4_2:51538130-51538149 Msa0605600:intron 35.0%
AAATAACCCATTCATCAAGC+TGG + chr4_2:51538139-51538158 None:intergenic 35.0%
!! ATACAATTTGGTTCTCTGAC+TGG + chr4_2:51538224-51538243 None:intergenic 35.0%
AAACATAATATGGGACACAC+TGG - chr4_2:51538259-51538278 Msa0605600:intron 35.0%
ATGTAGGGAGTCAATAATCT+AGG - chr4_2:51538346-51538365 Msa0605600:intron 35.0%
GGATCTATTCAAGATGAATG+AGG - chr4_2:51538561-51538580 Msa0605600:intron 35.0%
!!! GTGAAGTTATTTTTCCTCCA+AGG + chr4_2:51538638-51538657 None:intergenic 35.0%
CTTCAGGACTGAAAATTTGA+GGG + chr4_2:51538760-51538779 None:intergenic 35.0%
ACTTCAGGACTGAAAATTTG+AGG + chr4_2:51538761-51538780 None:intergenic 35.0%
CTTGTTAGAACTTAGACTTC+AGG + chr4_2:51538776-51538795 None:intergenic 35.0%
! TATGGATTACAATCAGCAAG+AGG + chr4_2:51538922-51538941 None:intergenic 35.0%
! ATTCTTTAGTCTTTGTCACC+GGG + chr4_2:51538978-51538997 None:intergenic 35.0%
TCCATAATTTCAATCCTTGC+TGG - chr4_2:51538998-51539017 Msa0605600:CDS 35.0%
CCATAATTTCAATCCTTGCT+GGG - chr4_2:51538999-51539018 Msa0605600:CDS 35.0%
AATGTAATGTGACAGCCATA+AGG + chr4_2:51539074-51539093 None:intergenic 35.0%
GGCTGTCACATTACATTATT+TGG - chr4_2:51539077-51539096 Msa0605600:intron 35.0%
AGTCTGTGATACTGAATGAT+GGG + chr4_2:51539106-51539125 None:intergenic 35.0%
CCAGAATTCAAAAGACTAAG+AGG + chr4_2:51539241-51539260 None:intergenic 35.0%
!! CCTCTTAGTCTTTTGAATTC+TGG - chr4_2:51539238-51539257 Msa0605600:CDS 35.0%
!! AATGGAAGGTTTTTGAGCGA+AGG + chr4_2:51536882-51536901 None:intergenic 40.0%
AGAAGAAAACAGAAGCGGAA+TGG + chr4_2:51536900-51536919 None:intergenic 40.0%
GCTTGAGAAGAAAACAGAAG+CGG + chr4_2:51536905-51536924 None:intergenic 40.0%
TTGTTCTACCTTGAAACACG+AGG + chr4_2:51536929-51536948 None:intergenic 40.0%
!! GCATTTTTCAGCTGTGATCA+TGG - chr4_2:51536950-51536969 Msa0605600:three_prime_UTR 40.0%
TAAGAAGAAGAGCAGAACTG+AGG + chr4_2:51537561-51537580 None:intergenic 40.0%
TGCTCTTCTTCTTAAGCTGT+TGG - chr4_2:51537567-51537586 Msa0605600:intron 40.0%
!! TCGAAACTAATTTCTAGCCG+CGG - chr4_2:51537664-51537683 Msa0605600:intron 40.0%
CGCGGATAATATTGCTTCAT+TGG - chr4_2:51537682-51537701 Msa0605600:intron 40.0%
GATTCATGAAGCAGTCTAGT+TGG + chr4_2:51537913-51537932 None:intergenic 40.0%
TCCCAAATCTCTCCAAACAA+AGG + chr4_2:51537951-51537970 None:intergenic 40.0%
!!! CAACAAGGTTGTTTTGGGAT+TGG - chr4_2:51538030-51538049 Msa0605600:intron 40.0%
ATATGGGACACACTGGAATA+AGG - chr4_2:51538266-51538285 Msa0605600:intron 40.0%
! TTTCTTTTCTGTCTCACCCT+TGG - chr4_2:51538618-51538637 Msa0605600:intron 40.0%
AGGGTGATTATCAAGCAAGT+TGG + chr4_2:51538741-51538760 None:intergenic 40.0%
CAAGAGGAAGTTAAATCTGG+AGG + chr4_2:51538906-51538925 None:intergenic 40.0%
CAGCAAGAGGAAGTTAAATC+TGG + chr4_2:51538909-51538928 None:intergenic 40.0%
! TTCTTTAGTCTTTGTCACCG+GGG + chr4_2:51538977-51538996 None:intergenic 40.0%
CCCAGCAAGGATTGAAATTA+TGG + chr4_2:51539002-51539021 None:intergenic 40.0%
GTGACAGCCATAAGGAAAAT+AGG + chr4_2:51539066-51539085 None:intergenic 40.0%
GAGTCTGTGATACTGAATGA+TGG + chr4_2:51539107-51539126 None:intergenic 40.0%
GACTAAGAGGTGAAACTGAA+AGG + chr4_2:51539228-51539247 None:intergenic 40.0%
GCTTGAAAACAATGCCTAGT+AGG - chr4_2:51539271-51539290 Msa0605600:exon 40.0%
GGTTAAACAAGGTTCCTACT+AGG + chr4_2:51539288-51539307 None:intergenic 40.0%
TGAGTAACTTATGACATCCG+GGG + chr4_2:51539309-51539328 None:intergenic 40.0%
CTGAGTAACTTATGACATCC+GGG + chr4_2:51539310-51539329 None:intergenic 40.0%
TCAGTAACCTTCTTCGGCAA+TGG - chr4_2:51536793-51536812 Msa0605600:three_prime_UTR 45.0%
!! TCTTGCCGCTGATTTACAGA+AGG + chr4_2:51536825-51536844 None:intergenic 45.0%
GAAAACAGAAGCGGAATGGA+AGG + chr4_2:51536896-51536915 None:intergenic 45.0%
TCAGCAATGGCAGTATGAAG+AGG + chr4_2:51537057-51537076 None:intergenic 45.0%
AGTGAGTCTAGCATCAGCAA+TGG + chr4_2:51537070-51537089 None:intergenic 45.0%
GAGAGGGGTAGTCTTTGATT+TGG - chr4_2:51537428-51537447 Msa0605600:three_prime_UTR 45.0%
GGTTGAATATGCATCAGACG+AGG - chr4_2:51537449-51537468 Msa0605600:three_prime_UTR 45.0%
CTGCTTCATGAATCCCTCAA+TGG - chr4_2:51537919-51537938 Msa0605600:intron 45.0%
GGGATTGGATGTAAGCAATC+TGG - chr4_2:51538045-51538064 Msa0605600:intron 45.0%
CTGGCAACTTCTGTTGCATT+TGG + chr4_2:51538205-51538224 None:intergenic 45.0%
TTTCAGATGGGCAACCGATA+TGG - chr4_2:51538443-51538462 Msa0605600:intron 45.0%
AAACATTGTGACAGGCTTGC+TGG - chr4_2:51538540-51538559 Msa0605600:intron 45.0%
TACTAGACTTTGCAGTTCCC+CGG - chr4_2:51538957-51538976 Msa0605600:CDS 45.0%
CTCTATAATGCAACCCAGCA+AGG + chr4_2:51539015-51539034 None:intergenic 45.0%
! GTAGGAACCTTGTTTAACCC+CGG - chr4_2:51539289-51539308 Msa0605600:five_prime_UTR 45.0%
ATGACATCCGGGGTTAAACA+AGG + chr4_2:51539299-51539318 None:intergenic 45.0%
GAAGAAGCCATTGCCGAAGA+AGG + chr4_2:51536803-51536822 None:intergenic 50.0%
CTGTAAATCAGCGGCAAGAG+TGG - chr4_2:51536826-51536845 Msa0605600:three_prime_UTR 50.0%
AGAACGATTCGCGAAGGAAG+TGG + chr4_2:51536855-51536874 None:intergenic 50.0%
!! GGTTCTAGAACGATTCGCGA+AGG + chr4_2:51536861-51536880 None:intergenic 50.0%
CTCAAGCTCCTCGTGTTTCA+AGG - chr4_2:51536918-51536937 Msa0605600:three_prime_UTR 50.0%
TCGCTTCCAATTCCACTTGC+TGG - chr4_2:51537097-51537116 Msa0605600:three_prime_UTR 50.0%
CATGCTCCAGCAAGTGGAAT+TGG + chr4_2:51537106-51537125 None:intergenic 50.0%
! TGAAAGCATGCTCCAGCAAG+TGG + chr4_2:51537112-51537131 None:intergenic 50.0%
TGCTGGAGCATGCTTTCACA+AGG - chr4_2:51537114-51537133 Msa0605600:three_prime_UTR 50.0%
! GAAGCAGTCTAGTTGGCTCT+AGG + chr4_2:51537906-51537925 None:intergenic 50.0%
! CTTTTCTGTCTCACCCTTGG+AGG - chr4_2:51538621-51538640 Msa0605600:intron 50.0%
Chromosome Type Strat End Strand Name
chr4_2 gene 51536762 51539329 51536762 ID=Msa0605600;Name=Msa0605600
chr4_2 mRNA 51536762 51539329 51536762 ID=Msa0605600-mRNA-1;Parent=Msa0605600;Name=Msa0605600-mRNA-1;_AED=0.00;_eAED=0.00;_QI=50|1|1|1|0.5|0.66|3|779|88
chr4_2 exon 51536762 51537560 51536762 ID=Msa0605600-mRNA-1:exon:12366;Parent=Msa0605600-mRNA-1
chr4_2 exon 51538956 51539074 51538956 ID=Msa0605600-mRNA-1:exon:12365;Parent=Msa0605600-mRNA-1
chr4_2 exon 51539152 51539329 51539152 ID=Msa0605600-mRNA-1:exon:12364;Parent=Msa0605600-mRNA-1
chr4_2 five_prime_UTR 51539280 51539329 51539280 ID=Msa0605600-mRNA-1:five_prime_utr;Parent=Msa0605600-mRNA-1
chr4_2 CDS 51539152 51539279 51539152 ID=Msa0605600-mRNA-1:cds;Parent=Msa0605600-mRNA-1
chr4_2 CDS 51538956 51539074 51538956 ID=Msa0605600-mRNA-1:cds;Parent=Msa0605600-mRNA-1
chr4_2 CDS 51537541 51537560 51537541 ID=Msa0605600-mRNA-1:cds;Parent=Msa0605600-mRNA-1
chr4_2 three_prime_UTR 51536762 51537540 51536762 ID=Msa0605600-mRNA-1:three_prime_utr;Parent=Msa0605600-mRNA-1
Gene Sequence

>Msa0605600

ATGGCTTCTTCCTTCTGTAAATCAGCGGCAAGAGTGGCTTCCACTTCCTTCGCGAATCGTTCTAGAACCTTCGCTCAAAAACCTTCCATTCCGCTTCTGTTTTCTTCTCAAGCTCCTCGTGTTTCAAGGATTTTATCGGTTGTTGGAAGTGTTGAATCATTGATGCCTCTTCATACTGCCATTGCTGATGCTAGACTCACTTCTAATATCGCTTCCAATTCCACTTGCTGGAGCATGCTTTCACAAGGACTTGAAAAAACATTGTGA
Protein sequence

>Msa0605600

MASSFCKSAARVASTSFANRSRTFAQKPSIPLLFSSQAPRVSRILSVVGSVESLMPLHTAIADARLTSNIASNSTCWSMLSQGLEKTL*