AlfalfaGEDB | Msa0491520

M. sativa cultivar zhongmu-4 / Msa0491520

KEGG: K17479
GO: GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
PFAM: PF00462.27

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0491520	XP_003598625.1	96.466	283	8	1	1	281	1	283	0.0	553

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0491520	sp\|Q9FLE8\|Y5986_ARATH	48.780	164	66	6	113	258	225	388	2.66e-41	149

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0491520	G7IYV7	96.466	283	8	1	1	281	1	283	0.0	553

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000490	Msa0491520	-0.842070	3.237360e-58	-8.615850e-47
Msa0000550	Msa0491520	-0.810192	1.302321e-50	-8.615850e-47
Msa0003560	Msa0491520	0.809549	1.790717e-50	-8.615850e-47
Msa0025740	Msa0491520	0.813822	2.107172e-51	-8.615850e-47
Msa0032610	Msa0491520	0.822443	2.363013e-53	-8.615850e-47
Msa0038020	Msa0491520	0.813981	1.943945e-51	-8.615850e-47
Msa0050730	Msa0491520	0.808485	3.026854e-50	-8.615850e-47
Msa0050760	Msa0491520	0.818699	1.710680e-52	-8.615850e-47
Msa0052030	Msa0491520	0.802984	4.327826e-49	-8.615850e-47
Msa0058270	Msa0491520	0.819183	1.327287e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0491520	MtrunA17_Chr3g0083531	96.466	283	8	1	1	281	1	283	0.0	553
Msa0491520	MtrunA17_Chr8g0352771	52.852	263	103	3	2	261	13	257	6.18e-87	259
Msa0491520	MtrunA17_Chr7g0256501	63.816	152	54	1	108	258	273	424	3.01e-69	220
Msa0491520	MtrunA17_Chr1g0171871	60.000	160	55	1	108	258	204	363	9.72e-67	212
Msa0491520	MtrunA17_Chr3g0105871	49.351	154	75	3	108	260	83	234	1.28e-49	164
Msa0491520	MtrunA17_Chr5g0437471	53.061	147	67	2	112	258	94	238	3.98e-49	163
Msa0491520	MtrunA17_Chr5g0431071	41.014	217	105	5	60	258	42	253	9.42e-47	157
Msa0491520	MtrunA17_Chr3g0113971	51.389	144	68	2	115	258	93	234	2.52e-45	153
Msa0491520	MtrunA17_Chr7g0266401	45.946	148	78	1	111	258	259	404	1.09e-40	145
Msa0491520	MtrunA17_Chr1g0196971	46.309	149	78	2	111	259	223	369	5.62e-40	142

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0491520	AT5G13810.1	56.420	257	103	5	6	259	23	273	7.00e-95	281
Msa0491520	AT2G41330.1	56.442	163	69	2	98	258	239	401	2.04e-55	184
Msa0491520	AT3G57070.1	44.215	242	127	3	20	258	179	415	8.40e-55	183
Msa0491520	AT5G58530.1	42.922	219	105	6	54	259	55	266	1.35e-49	165
Msa0491520	AT5G39865.1	48.780	164	66	6	113	258	225	388	2.70e-42	149
Msa0491520	AT5G06470.1	41.618	173	86	4	99	259	70	239	7.76e-41	141
Msa0491520	AT3G28850.1	42.614	176	71	3	113	258	251	426	1.70e-38	140
Msa0491520	AT4G10630.1	46.584	161	74	4	111	259	174	334	2.24e-38	137
Msa0491520	AT1G32760.1	49.689	161	73	4	107	259	154	314	2.35e-38	137
Msa0491520	AT3G11773.1	46.479	142	73	3	118	259	12	150	4.46e-36	126

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Find 75 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAGTGAAATGAAATGTG+TGG	0.656253	3_4:-14266278	None:intergenic
AAAGGGTATGTTGTTTCGGT+TGG	0.494457	3_4:+14266838	Msa0491520:CDS
AAATTATTTGATGAGGATGA+AGG	0.476639	3_4:+14268293	Msa0491520:CDS
AACAAAGCCGGGCTTCATCT+TGG	0.384748	3_4:-14268213	None:intergenic
AACGAATTTCTAGACCGGTC+CGG	0.532633	3_4:+14266611	Msa0491520:CDS
AAGCAAATTCTACGGTTCAA+TGG	0.458730	3_4:+14266640	Msa0491520:CDS
AAGCGATTATGGTTGCAGCC+CGG	0.607773	3_4:+14268512	Msa0491520:three_prime_UTR
ACAAAACAAAACGATGTTCA+AGG	0.501444	3_4:-14268566	None:intergenic
ACAAAGCCGGGCTTCATCTT+GGG	0.437737	3_4:-14268212	None:intergenic
ACAACTCTCGCAAACAAAGC+CGG	0.469766	3_4:-14268225	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGGATTTGAAAGAAAGAAGT+TGG	-	chr3_4:14266258-14266277	None:intergenic	30.0%
AAAGAGTGAAATGAAATGTG+TGG	-	chr3_4:14266281-14266300	None:intergenic	30.0%
TCAATGAAATCATCTTTACG+TGG	+	chr3_4:14266665-14266684	Msa0491520:CDS	30.0%
TGATGTTACGATAGATATGT+CGG	+	chr3_4:14267185-14267204	Msa0491520:intron	30.0%
TTAACGTATGGTGAAAAATG+AGG	+	chr3_4:14267380-14267399	Msa0491520:intron	30.0%
TAACGTATGGTGAAAAATGA+GGG	+	chr3_4:14267381-14267400	Msa0491520:intron	30.0%
GATCAAGATTAGACAACTAA+AGG	-	chr3_4:14267668-14267687	None:intergenic	30.0%
ATGAACTAGGGAAATTGATT+TGG	+	chr3_4:14267747-14267766	Msa0491520:intron	30.0%
TCTGATATCAAATTATCAGC+AGG	+	chr3_4:14267861-14267880	Msa0491520:intron	30.0%
TTGAAACTGGTGAATTGAAA+AGG	+	chr3_4:14268174-14268193	Msa0491520:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr3_4	gene	14266214	14268792	14266214	ID=Msa0491520;Name=Msa0491520
chr3_4	mRNA	14266214	14268792	14266214	ID=Msa0491520-mRNA-1;Parent=Msa0491520;Name=Msa0491520-mRNA-1;_AED=0.02;_eAED=0.02;_QI=322\|1\|1\|1\|1\|1\|2\|347\|281
chr3_4	exon	14266214	14267021	14266214	ID=Msa0491520-mRNA-1:exon:4191;Parent=Msa0491520-mRNA-1
chr3_4	exon	14268086	14268792	14268086	ID=Msa0491520-mRNA-1:exon:4192;Parent=Msa0491520-mRNA-1
chr3_4	five_prime_UTR	14266214	14266535	14266214	ID=Msa0491520-mRNA-1:five_prime_utr;Parent=Msa0491520-mRNA-1
chr3_4	CDS	14266536	14267021	14266536	ID=Msa0491520-mRNA-1:cds;Parent=Msa0491520-mRNA-1
chr3_4	CDS	14268086	14268445	14268086	ID=Msa0491520-mRNA-1:cds;Parent=Msa0491520-mRNA-1
chr3_4	three_prime_UTR	14268446	14268792	14268446	ID=Msa0491520-mRNA-1:three_prime_utr;Parent=Msa0491520-mRNA-1

Gene Sequence

>Msa0491520

ATGTCGAGGTTTCAATTCTTCAACCGATCAAACACGATGCACACGTCATCAAAAGAACAATCACAGAAACCATTCAACGAATTTCTAGACCGGTCCGGTTCGTTAAGCAAATTCTACGGTTCAATGGAGTCAATGAAATCATCTTTACGTGGAAGAATGGTGAAGAAGCTTTGCACTTTATTTGAATCACCACCGACGACGAAAGAATTATCATCCTCGTCGTCGAATTTAAAACCTTCTTCTGAACCACGCGCGGCTTCGAAGTTAGGTCAGAAGCCGCGTTCTGGTTCGGAAGAAGATGAAAAGGGTATGTTGTTTCGGTTGGCGGATGCTGATGATCGGATTGTGGTGTATTTGACGAGTTTGAGAGGGATAAGAAGAACGTTTGAGGATTGTAATGCTGTTAAGATGATTTTGAAAGGGTTTAGGGTTTGGGTTGATGAAAGAGATGTTTCTATGGATCGTGCTTTCAGAAAAGAATTGCAGTCTGTGATGGGTGAGGAAAACGTGACACTGCCTCAAGTGTTCGTTAGAGGAAAGTACATTGGTGGTGCTGATGTGATCAAATCTCTATTTGAAACTGGTGAATTGAAAAGGATTCTTGAAGGTTTTCCCAAGATGAAGCCCGGCTTTGTTTGCGAGAGTTGTGGCGATGCTAGGTTTATACCTTGTGAGAATTGCAGTGGGAGTAGGAAATTATTTGATGAGGATGAAGGTTTGTTGAAAAGATGCTTGGAGTGCAATGAGAATGGACTTGTAAGGTGTCCTTGTTGCGCCTGTTCTGGAATGATGTTCTGTGATTATGAAAACGAGGGCGATGCTGTGTATCGATCCTTTATATTGTAA

Protein sequence

>Msa0491520

MSRFQFFNRSNTMHTSSKEQSQKPFNEFLDRSGSLSKFYGSMESMKSSLRGRMVKKLCTLFESPPTTKELSSSSSNLKPSSEPRAASKLGQKPRSGSEEDEKGMLFRLADADDRIVVYLTSLRGIRRTFEDCNAVKMILKGFRVWVDERDVSMDRAFRKELQSVMGEENVTLPQVFVRGKYIGGADVIKSLFETGELKRILEGFPKMKPGFVCESCGDARFIPCENCSGSRKLFDEDEGLLKRCLECNENGLVRCPCCACSGMMFCDYENEGDAVYRSFIL*