AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0261720

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0261720 MtrunA17_Chr2g0324061 94.583 480 16 2 1 480 1 470 0.0 927
Msa0261720 MtrunA17_Chr4g0022171 79.835 486 76 5 1 480 1 470 0.0 764
Msa0261720 MtrunA17_Chr7g0249421 50.110 453 192 6 45 477 50 488 2.05e-146 428
Msa0261720 MtrunA17_Chr7g0221691 48.244 427 194 8 1 416 42 452 1.27e-130 386
Msa0261720 MtrunA17_Chr7g0243571 43.839 422 188 9 56 474 57 432 1.70e-115 347
Msa0261720 MtrunA17_Chr8g0342431 46.556 363 130 7 45 373 40 372 3.29e-102 311
Msa0261720 MtrunA17_Chr4g0067711 47.436 312 141 5 63 373 63 352 2.18e-101 308
Msa0261720 MtrunA17_Chr1g0192421 45.625 320 151 4 55 373 53 350 8.46e-101 306
Msa0261720 MtrunA17_Chr7g0272061 45.317 331 161 5 55 381 55 369 9.94e-101 306
Msa0261720 MtrunA17_Chr2g0295401 47.452 314 142 7 61 373 62 353 6.71e-100 304
Msa0261720 MtrunA17_Chr4g0004971 45.427 328 154 5 51 374 38 344 6.88e-100 304
Msa0261720 MtrunA17_Chr1g0151781 46.894 322 148 4 55 376 33 331 2.44e-88 274
Msa0261720 MtrunA17_Chr4g0066371 27.329 322 168 10 73 375 68 342 6.66e-25 106
Msa0261720 MtrunA17_Chr6g0486661 29.846 325 159 12 73 375 106 383 8.61e-25 108
Msa0261720 MtrunA17_Chr5g0396731 31.353 303 141 10 44 335 54 300 1.11e-23 102
Msa0261720 MtrunA17_Chr2g0286951 28.182 330 171 11 73 383 69 351 6.39e-22 97.8
Msa0261720 MtrunA17_Chr3g0135961 27.653 311 160 12 25 332 236 484 4.25e-18 87.0
Msa0261720 MtrunA17_Chr3g0112291 30.357 280 123 12 68 333 100 321 4.51e-18 85.9
Msa0261720 MtrunA17_Chr5g0435721 35.294 187 83 7 160 333 258 419 1.13e-17 85.5
Msa0261720 MtrunA17_Chr3g0136711 31.111 225 119 8 161 382 100 291 1.99e-17 82.8
Msa0261720 MtrunA17_Chr6g0483411 29.565 230 114 8 161 372 119 318 2.93e-17 82.8
Msa0261720 MtrunA17_Chr1g0150451 32.984 191 101 6 161 350 143 307 1.22e-16 81.3
Msa0261720 MtrunA17_Chr1g0212961 29.389 262 118 9 90 350 126 321 2.20e-16 80.5
Msa0261720 MtrunA17_Chr4g0070871 27.755 245 127 8 88 332 251 445 6.05e-16 80.1
Msa0261720 MtrunA17_Chr2g0299981 27.907 258 152 7 76 328 248 476 6.06e-15 77.4
Msa0261720 MtrunA17_Chr2g0279151 32.461 191 102 8 163 352 102 266 2.57e-14 73.2
Msa0261720 MtrunA17_Chr3g0136811 28.814 295 149 13 65 333 28 287 1.29e-13 72.0
Msa0261720 MtrunA17_Chr1g0150781 25.392 319 168 11 17 332 237 488 1.59e-13 72.8
Msa0261720 MtrunA17_Chr3g0115851 33.019 212 101 11 166 372 106 281 2.19e-13 70.5
Msa0261720 MtrunA17_Chr1g0175381 26.444 329 174 16 87 386 76 365 2.59e-13 71.6
Msa0261720 MtrunA17_Chr2g0317021 34.392 189 89 9 187 373 177 332 7.65e-13 69.7
Msa0261720 MtrunA17_Chr8g0360351 32.653 196 102 9 159 350 96 265 9.47e-13 68.6
Msa0261720 MtrunA17_Chr1g0158781 31.609 174 92 5 160 333 184 330 1.07e-12 69.7
Msa0261720 MtrunA17_Chr1g0150071 27.931 290 158 12 65 333 28 287 3.00e-12 68.2
Msa0261720 MtrunA17_Chr3g0126511 30.688 189 104 5 163 350 162 324 5.06e-12 67.4
Msa0261720 MtrunA17_Chr5g0407161 25.275 273 125 10 90 350 124 329 6.55e-12 67.0
Msa0261720 MtrunA17_Chr4g0051661 32.984 191 98 8 162 350 219 381 6.79e-12 67.0
Msa0261720 MtrunA17_Chr3g0144111 29.379 177 84 7 163 332 47 189 1.86e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0261720 AT4G03415.1 72.926 458 103 6 21 477 28 465 0.0 629
Msa0261720 AT4G03415.3 72.926 458 103 6 21 477 28 465 0.0 629
Msa0261720 AT4G03415.2 72.926 458 103 6 21 477 28 465 0.0 629
Msa0261720 AT1G03590.1 66.112 481 143 4 1 480 1 462 0.0 623
Msa0261720 AT1G16220.1 48.899 454 188 10 42 473 46 477 1.61e-143 421
Msa0261720 AT3G02750.2 46.918 503 222 8 1 473 1 488 4.66e-143 419
Msa0261720 AT3G02750.1 46.918 503 222 8 1 473 1 488 4.66e-143 419
Msa0261720 AT1G79630.1 48.115 451 188 9 51 473 58 490 2.79e-141 415
Msa0261720 AT1G79630.8 48.115 451 188 9 51 473 54 486 4.76e-141 414
Msa0261720 AT5G36250.2 47.111 450 173 8 45 473 52 457 1.06e-137 405
Msa0261720 AT5G36250.1 46.742 445 168 8 45 473 52 443 6.57e-135 397
Msa0261720 AT3G02750.3 43.866 538 222 9 1 473 1 523 1.19e-134 399
Msa0261720 AT1G79630.2 47.268 421 176 9 81 473 18 420 1.43e-125 373
Msa0261720 AT1G79630.7 47.268 421 176 9 81 473 18 420 1.43e-125 373
Msa0261720 AT1G79630.6 45.980 398 170 8 103 473 1 380 1.37e-111 335
Msa0261720 AT1G79630.3 45.980 398 170 8 103 473 1 380 1.37e-111 335
Msa0261720 AT1G79630.4 45.980 398 170 8 103 473 1 380 1.37e-111 335
Msa0261720 AT1G79630.5 45.980 398 170 8 103 473 1 380 1.37e-111 335
Msa0261720 AT5G36250.3 53.357 283 119 3 45 314 52 334 3.40e-106 320
Msa0261720 AT5G01700.2 48.624 327 141 5 52 376 39 340 2.00e-101 309
Msa0261720 AT3G05640.2 45.511 323 148 4 52 373 54 349 3.33e-101 308
Msa0261720 AT3G05640.1 45.511 323 148 4 52 373 54 349 3.33e-101 308
Msa0261720 AT3G05640.3 45.511 323 148 4 52 373 54 349 3.33e-101 308
Msa0261720 AT5G01700.1 50.160 313 129 5 66 376 4 291 5.78e-101 306
Msa0261720 AT5G27930.1 46.708 319 148 5 55 372 56 353 1.22e-100 306
Msa0261720 AT5G27930.2 46.708 319 148 5 55 372 56 353 1.22e-100 306
Msa0261720 AT5G27930.3 45.769 260 128 4 56 314 57 304 2.57e-82 258
Msa0261720 AT3G16800.2 41.304 322 158 4 54 373 56 348 3.93e-82 258
Msa0261720 AT3G16800.1 41.304 322 158 4 54 373 56 348 3.93e-82 258
Msa0261720 AT3G16800.3 41.304 322 158 4 54 373 56 348 3.93e-82 258
Msa0261720 AT4G32950.1 40.731 383 164 10 1 380 1 323 3.21e-77 244
Msa0261720 AT5G26010.1 41.433 321 150 6 59 376 42 327 1.85e-75 240
Msa0261720 AT3G16800.4 40.426 282 138 3 93 373 1 253 1.34e-69 223
Msa0261720 AT3G16800.6 40.426 282 138 3 93 373 1 253 1.34e-69 223
Msa0261720 AT4G32950.2 40.952 315 132 9 1 312 1 264 2.49e-60 199
Msa0261720 AT3G16800.5 35.870 184 96 3 54 235 56 219 5.11e-33 125
Msa0261720 AT2G20050.2 30.205 341 166 12 73 393 125 413 3.32e-28 119
Msa0261720 AT2G20050.1 30.205 341 166 12 73 393 125 413 3.96e-28 119
Msa0261720 AT3G11410.1 32.746 284 108 13 68 327 112 336 1.62e-20 94.0
Msa0261720 AT5G59220.1 31.522 276 127 11 68 333 119 342 6.56e-20 92.0
Msa0261720 AT3G06270.1 26.557 305 153 11 73 356 70 324 1.56e-19 90.1
Msa0261720 AT1G07430.1 29.355 310 162 12 34 333 92 354 8.09e-19 89.4
Msa0261720 AT5G10740.1 34.359 195 97 6 161 352 99 265 5.16e-18 85.9
Msa0261720 AT5G10740.2 34.359 195 97 6 161 352 99 265 5.16e-18 85.9
Msa0261720 AT4G28400.3 36.508 189 93 9 163 350 82 244 6.89e-18 84.0
Msa0261720 AT5G24940.1 35.632 174 85 6 161 333 99 246 7.30e-18 86.3
Msa0261720 AT4G28400.1 36.508 189 93 9 163 350 104 266 1.11e-17 83.6
Msa0261720 AT4G31750.2 32.093 215 110 8 163 374 101 282 7.94e-17 81.6
Msa0261720 AT4G31750.1 31.925 213 113 7 163 374 101 282 9.83e-17 81.3
Msa0261720 AT4G31750.3 31.925 213 113 7 163 374 101 282 9.83e-17 81.3
Msa0261720 AT1G72770.4 32.806 253 116 10 90 332 238 446 3.09e-16 81.6
Msa0261720 AT1G72770.1 32.806 253 116 10 90 332 238 446 3.09e-16 81.6
Msa0261720 AT1G72770.3 32.806 253 116 10 90 332 238 446 3.09e-16 81.6
Msa0261720 AT2G29380.1 30.175 285 119 14 68 332 85 309 6.65e-16 79.7
Msa0261720 AT1G78200.5 33.161 193 105 6 161 352 54 223 7.56e-15 74.3
Msa0261720 AT1G78200.4 33.161 193 105 6 161 352 54 223 7.56e-15 74.3
Msa0261720 AT1G43900.1 32.338 201 108 6 149 348 177 350 1.60e-14 75.5
Msa0261720 AT1G17550.1 29.134 254 117 9 90 328 237 442 6.29e-14 74.3
Msa0261720 AT1G17550.2 29.134 254 117 9 90 328 237 442 6.29e-14 74.3
Msa0261720 AT2G20630.1 32.843 204 110 9 148 350 85 262 9.73e-14 72.0
Msa0261720 AT2G20630.2 32.843 204 110 9 148 350 85 262 1.30e-13 71.6
Msa0261720 AT5G53140.1 32.727 165 84 5 187 350 193 331 2.94e-13 72.0
Msa0261720 AT5G53140.2 32.727 165 84 5 187 350 193 331 2.94e-13 72.0
Msa0261720 AT4G31860.1 27.835 291 157 11 65 333 28 287 1.22e-12 69.7
Msa0261720 AT4G31860.3 27.835 291 157 11 65 333 28 287 1.94e-12 68.2
Msa0261720 AT3G15260.2 32.995 197 102 9 158 350 102 272 4.68e-12 67.0
Msa0261720 AT3G15260.1 32.995 197 102 9 158 350 102 272 4.68e-12 67.0
Msa0261720 AT4G28400.2 36.076 158 81 9 163 318 104 243 7.49e-12 65.9
Msa0261720 AT1G18030.1 26.957 230 120 8 161 372 149 348 1.10e-11 66.6
Msa0261720 AT2G25070.1 27.055 292 158 12 65 333 28 287 1.74e-11 65.9
Msa0261720 AT2G25070.2 27.055 292 158 12 65 333 28 287 1.74e-11 65.9
Msa0261720 AT2G34740.3 36.306 157 79 9 159 311 145 284 2.83e-11 65.1
Msa0261720 AT2G34740.2 36.943 157 78 10 159 311 163 302 3.62e-11 64.7
Msa0261720 AT1G07160.1 31.429 175 93 6 158 332 188 335 9.66e-11 63.9

Find 124 sgRNAs with CRISPR-Local

Find 183 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAGCTTTATGTCCTTCT+TGG 0.199638 2_2:+60293588 None:intergenic
GCTGAAGTCTCATGCAAATT+TGG 0.220869 2_2:-60293470 Msa0261720:CDS
TTCTATAGTGAGAGGATCTT+TGG 0.223060 2_2:-60294265 Msa0261720:five_prime_UTR
TAAATATCCAACTTCGAAAA+TGG 0.246853 2_2:-60292420 Msa0261720:CDS
GATGAGGTCAATGTGAATTC+TGG 0.264414 2_2:-60294577 Msa0261720:five_prime_UTR
AATTTAATATTTAATATATA+TGG 0.273123 2_2:+60294417 None:intergenic
TGCCTGAATGCCTGTTGCTT+TGG 0.290415 2_2:+60292306 None:intergenic
AATTTGTTCATGGGTAATAT+TGG 0.292320 2_2:-60293157 Msa0261720:CDS
TTATCCATTCATATAGCTTT+CGG 0.319034 2_2:-60294364 Msa0261720:five_prime_UTR
ACCTAGTAGCACGCAAAGTT+AGG 0.335302 2_2:-60293687 Msa0261720:CDS
TTGAAAATTGTTTATTCAGC+TGG 0.344033 2_2:-60292048 Msa0261720:exon
TATGACACACTTGGCAAATC+AGG 0.348941 2_2:+60293058 None:intergenic
TGACCATCATCTGACTCAAC+TGG 0.351058 2_2:+60292281 None:intergenic
GTGAGAGGATCTTTGGAATC+AGG 0.358603 2_2:-60294258 Msa0261720:five_prime_UTR
TCTAGATGTTCATTTAATCT+TGG 0.380127 2_2:-60294712 Msa0261720:five_prime_UTR
ATCATTGGCAGCGAGGGTTC+TGG 0.380369 2_2:-60292471 Msa0261720:CDS
TTAGCAATGGCTAGAGCATT+TGG 0.381409 2_2:-60292847 Msa0261720:CDS
ACTCCATCTTGTCTTGAAAT+CGG 0.382346 2_2:-60294065 Msa0261720:CDS
TCAGGGTTCAAATTTGTTCA+TGG 0.382599 2_2:-60293167 Msa0261720:intron
ATCAAGATGCCATGATTGTA+TGG 0.384111 2_2:-60293896 Msa0261720:CDS
CCATTACTAAATATTCTGTT+TGG 0.386029 2_2:+60293969 None:intergenic
TTCTTGTATATTTACGCAGC+AGG 0.386376 2_2:-60293934 Msa0261720:CDS
CACACAAATTTAGATATTTG+TGG 0.393174 2_2:+60294686 None:intergenic
ACATGTGTAGGAGGAGGAAA+AGG 0.396810 2_2:-60292200 Msa0261720:CDS
AACCACACCCTACAAACTTC+TGG 0.399419 2_2:+60292892 None:intergenic
TTTACTCAATTGACCAAGCT+TGG 0.403894 2_2:-60294231 Msa0261720:five_prime_UTR
CCCTAACTTTGCGTGCTACT+AGG 0.407833 2_2:+60293686 None:intergenic
CATCAAGTCATTGCTGTCCT+TGG 0.412532 2_2:+60293111 None:intergenic
CTATTAAATTAAATCAAATC+CGG 0.415351 2_2:+60294618 None:intergenic
CAAGGACAGCAATGACTTGA+TGG 0.418227 2_2:-60293110 Msa0261720:CDS
TGAATGCCTGTTGCTTTGGA+TGG 0.421566 2_2:+60292310 None:intergenic
ATCCAAAGCAACAGGCATTC+AGG 0.423868 2_2:-60292308 Msa0261720:CDS
AGAAGCCGGTGCGTAAATTC+AGG 0.424088 2_2:+60292787 None:intergenic
CAAAACTGGTTAGGTCTAGA+AGG 0.426937 2_2:-60292131 Msa0261720:CDS
CATCAGCTGAAGATCAAAAC+TGG 0.432537 2_2:-60292145 Msa0261720:CDS
AGGATGAACCAGAAGTTTGT+AGG 0.433167 2_2:-60292900 Msa0261720:CDS
ACTAAATATTCTGTTTGGTA+AGG 0.434667 2_2:+60293974 None:intergenic
TTCATTGTTCTTGCCTCGGA+TGG 0.438125 2_2:-60292748 Msa0261720:CDS
TTGTAATGCTTAAAACAATA+CGG 0.440204 2_2:-60292016 Msa0261720:three_prime_UTR
TGGTGATTCAAGAGCAATCA+TGG 0.443050 2_2:-60293137 Msa0261720:CDS
ATGAGGTCAATGTGAATTCT+GGG 0.447942 2_2:-60294576 Msa0261720:five_prime_UTR
GTGAAGTTCCTTCGCAAAGA+TGG 0.450703 2_2:+60292248 None:intergenic
AATTCACACCTTGTTGCTGT+TGG 0.452894 2_2:+60294302 None:intergenic
GCTGAAGATCAAAACTGGTT+AGG 0.453850 2_2:-60292140 Msa0261720:CDS
AAACTCTGAGATTCCAAGCT+TGG 0.454604 2_2:+60294218 None:intergenic
CCGCAACCATCCAAAGCAAC+AGG 0.458483 2_2:-60292316 Msa0261720:CDS
ATCAGCACCAAGTCGATCAT+TGG 0.460709 2_2:-60292486 Msa0261720:CDS
CAGGGTTCAAATTTGTTCAT+GGG 0.472932 2_2:-60293166 Msa0261720:CDS
TGTGTCTTCTTCTGTTCAGC+CGG 0.478288 2_2:-60294392 Msa0261720:five_prime_UTR
TTTGCCGAAAGCTATATGAA+TGG 0.481098 2_2:+60294360 None:intergenic
AGACTTCAGCTCTTTGTCCA+TGG 0.483435 2_2:+60293483 None:intergenic
CCAAACAGAATATTTAGTAA+TGG 0.485067 2_2:-60293969 Msa0261720:CDS
ATATGAATGGATAAAGAAAC+CGG 0.487229 2_2:+60294373 None:intergenic
AACCAGAAGTTTGTAGGGTG+TGG 0.487786 2_2:-60292894 Msa0261720:CDS
ATTTACGCAGCAGGGTCGTA+AGG 0.489572 2_2:-60293925 Msa0261720:CDS
GGCCGTTCTTCAGAAAATCT+AGG 0.491219 2_2:+60292077 None:intergenic
CAAGGGTAGGGTATTTGCAT+TGG 0.493438 2_2:-60292923 Msa0261720:CDS
GGGTAGGGTATTTGCATTGG+AGG 0.495563 2_2:-60292920 Msa0261720:CDS
ATCTGTGACTGTAGTGAAGC+AGG 0.498133 2_2:-60293425 Msa0261720:intron
TCAGTTCATTGTTCTTGCCT+CGG 0.500431 2_2:-60292752 Msa0261720:CDS
TGCGGTGTGTTCGATGGCCA+TGG 0.500762 2_2:-60293721 Msa0261720:CDS
TGTGTAGGAGGAGGAAAAGG+AGG 0.506060 2_2:-60292197 Msa0261720:CDS
TCTAGCTTCATTCTTAATGT+CGG 0.507918 2_2:+60291913 None:intergenic
AGCTTTCATGAAGGCATACA+AGG 0.511427 2_2:-60293506 Msa0261720:CDS
TAATTTCTCTGCTGAGGATG+AGG 0.513150 2_2:-60293548 Msa0261720:CDS
CAAGAACAATGAACTGATCT+CGG 0.513831 2_2:+60292758 None:intergenic
CCTAGTAGCACGCAAAGTTA+GGG 0.515838 2_2:-60293686 Msa0261720:CDS
TTCGATGGCCATGGTCCATA+CGG 0.524180 2_2:-60293712 Msa0261720:CDS
GCTGTACCAGTGAGTTTACT+CGG 0.525049 2_2:+60292102 None:intergenic
ATCTTCTGCGGTGTGTTCGA+TGG 0.528423 2_2:-60293727 Msa0261720:CDS
GCTCTAGCCATTGCTAATCC+AGG 0.531526 2_2:+60292853 None:intergenic
AATCCAGGTGCATCATCAAA+AGG 0.541135 2_2:+60292868 None:intergenic
TTAATACTTTCTATAGTGAG+AGG 0.541580 2_2:-60294273 Msa0261720:five_prime_UTR
TGAGGTGAGTTCTATGTGGA+GGG 0.545772 2_2:-60293530 Msa0261720:CDS
GTTCCTTCGCAAAGATGGCT+CGG 0.550410 2_2:+60292253 None:intergenic
CTCGCTGCCAATGATCGACT+TGG 0.550840 2_2:+60292479 None:intergenic
AAGAGCAATCATGGGATCCA+AGG 0.551093 2_2:-60293128 Msa0261720:CDS
GATCTCGGTCTGTGAGAAGC+CGG 0.551873 2_2:+60292773 None:intergenic
ACTTACTTCCCATACAATCA+TGG 0.552828 2_2:+60293887 None:intergenic
TATAATGAGACATGTGTAGG+AGG 0.553514 2_2:-60292209 Msa0261720:CDS
AAGGACATAAAGCTTGAAAG+TGG 0.554778 2_2:-60293583 Msa0261720:CDS
TATTAAATTAAATCAAATCC+GGG 0.554787 2_2:+60294619 None:intergenic
AAGTCGATCATTGGCAGCGA+GGG 0.560312 2_2:-60292477 Msa0261720:CDS
CCTGTTGCTTTGGATGGTTG+CGG 0.563383 2_2:+60292316 None:intergenic
GGATGAACCAGAAGTTTGTA+GGG 0.564180 2_2:-60292899 Msa0261720:CDS
GAAATTTCTTACACCATCCG+AGG 0.565735 2_2:+60292735 None:intergenic
ATGAGGTGAGTTCTATGTGG+AGG 0.571277 2_2:-60293531 Msa0261720:CDS
AATCCAGTTGAGTCAGATGA+TGG 0.571512 2_2:-60292284 Msa0261720:CDS
TGGAATCTGCCGCACGAGAG+TGG 0.576177 2_2:-60292448 Msa0261720:CDS
AATGAGACATGTGTAGGAGG+AGG 0.580853 2_2:-60292206 Msa0261720:CDS
TCTTGTATATTTACGCAGCA+GGG 0.584645 2_2:-60293933 Msa0261720:CDS
AATCCGATTTCAAGACAAGA+TGG 0.585814 2_2:+60294062 None:intergenic
ACATGTCTCATTATATTCTG+AGG 0.588314 2_2:+60292217 None:intergenic
AATTAGTTTACATTAAGCAA+AGG 0.589217 2_2:-60294455 Msa0261720:five_prime_UTR
TCAAGATGCCATGATTGTAT+GGG 0.591058 2_2:-60293895 Msa0261720:CDS
CAATAGAAGTAATGACGGCT+CGG 0.592065 2_2:-60294090 Msa0261720:CDS
GTCAATGTGAATTCTGGGGA+AGG 0.595148 2_2:-60294571 Msa0261720:five_prime_UTR
TGAGAATAATTTCTCTGCTG+AGG 0.599889 2_2:-60293554 Msa0261720:CDS
ATTGGCAGCGAGGGTTCTGG+TGG 0.601499 2_2:-60292468 Msa0261720:CDS
CGTGCTACTAGGTGACCGTA+TGG 0.603429 2_2:+60293697 None:intergenic
CTGTACCTGAATTTACGCAC+CGG 0.609120 2_2:-60292792 Msa0261720:CDS
TTTACGCAGCAGGGTCGTAA+GGG 0.610094 2_2:-60293924 Msa0261720:CDS
CATGAAGGCATACAAGGCCA+TGG 0.610791 2_2:-60293500 Msa0261720:CDS
ACAAGCAATAGAAGTAATGA+CGG 0.615043 2_2:-60294095 Msa0261720:CDS
AGTCCGAGCCATCTTTGCGA+AGG 0.615130 2_2:-60292256 Msa0261720:CDS
GAATATAATGAGACATGTGT+AGG 0.617364 2_2:-60292212 Msa0261720:CDS
GAGTCTGTCGATGTTGAAGA+CGG 0.621885 2_2:-60292173 Msa0261720:CDS
GGTGATTCAAGAGCAATCAT+GGG 0.631261 2_2:-60293136 Msa0261720:CDS
CTGTACCAGTGAGTTTACTC+GGG 0.631787 2_2:+60292103 None:intergenic
TATTTAGTAATGGAAAGAGT+CGG 0.646864 2_2:-60293959 Msa0261720:CDS
CAAGTCGATCATTGGCAGCG+AGG 0.650429 2_2:-60292478 Msa0261720:CDS
GCTAGTTTCCAACAGCAACA+AGG 0.652102 2_2:-60294310 Msa0261720:five_prime_UTR
GTGGAGGGAAGCTTTCATGA+AGG 0.654164 2_2:-60293515 Msa0261720:CDS
TCAGAAGATGCGATCTTCTG+CGG 0.654370 2_2:-60293739 Msa0261720:CDS
TGTCTTGAAATCGGATTGTG+TGG 0.666044 2_2:-60294056 Msa0261720:CDS
TGTCACCCGAGTAAACTCAC+TGG 0.671131 2_2:-60292108 Msa0261720:CDS
ACATTGACCTCATCGAAATG+CGG 0.674333 2_2:+60294586 None:intergenic
CATAAAGCTTGAAAGTGGAG+AGG 0.675886 2_2:-60293578 Msa0261720:CDS
AGGATGAGGTGAGTTCTATG+TGG 0.676000 2_2:-60293534 Msa0261720:CDS
CTAGGTGACCGTATGGACCA+TGG 0.681676 2_2:+60293704 None:intergenic
TGATGCACCTGGATTAGCAA+TGG 0.684010 2_2:-60292860 Msa0261720:CDS
TGAGGTCAATGTGAATTCTG+GGG 0.688531 2_2:-60294575 Msa0261720:five_prime_UTR
TTTAAGTTTCCACTCTCGTG+CGG 0.690625 2_2:+60292439 None:intergenic
TCAAACTCCGCATTTCGATG+AGG 0.703059 2_2:-60294593 Msa0261720:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAATATTTAATATATA+TGG + chr2_2:60292377-60292396 None:intergenic 0.0%
!! AAAAAACATAAATTTAAACA+TGG + chr2_2:60293775-60293794 None:intergenic 10.0%
!! TCATAAATATTATTTATATG+AGG - chr2_2:60294141-60294160 Msa0261720:five_prime_UTR 10.0%
!!! AAGAAGAATTATTTTTTATT+TGG - chr2_2:60294805-60294824 Msa0261720:intron 10.0%
!!! AAAAATGTTTTATTCTCATT+TGG - chr2_2:60291993-60292012 Msa0261720:three_prime_UTR 15.0%
!! TATTAAATTAAATCAAATCC+GGG + chr2_2:60292175-60292194 None:intergenic 15.0%
!! CTATTAAATTAAATCAAATC+CGG + chr2_2:60292176-60292195 None:intergenic 15.0%
!!! TTTTGATTTTTGTTTTCTTT+AGG - chr2_2:60293021-60293040 Msa0261720:intron 15.0%
!!! AAAAATGAAAGCAATTTTAT+CGG + chr2_2:60293139-60293158 None:intergenic 15.0%
!!! AATTAGTTTACATTAAGCAA+AGG - chr2_2:60292336-60292355 Msa0261720:CDS 20.0%
!!! ATCTAAAGTGTTTTTCTTTT+TGG - chr2_2:60292645-60292664 Msa0261720:intron 20.0%
!!! TCTAAAGTGTTTTTCTTTTT+GGG - chr2_2:60292646-60292665 Msa0261720:intron 20.0%
!! AGAAAACAAAAATCAAAACT+AGG + chr2_2:60293019-60293038 None:intergenic 20.0%
!!! GATAAAATTGCTTTCATTTT+TGG - chr2_2:60293138-60293157 Msa0261720:CDS 20.0%
!! TGTTCAAAAGAAAACATATA+CGG + chr2_2:60293564-60293583 None:intergenic 20.0%
!!! TATATGTTTTCTTTTGAACA+AGG - chr2_2:60293564-60293583 Msa0261720:CDS 20.0%
!!! ATATGTTTTCTTTTGAACAA+GGG - chr2_2:60293565-60293584 Msa0261720:CDS 20.0%
!!! TTATTTTCTTCACTTTTTCA+GGG - chr2_2:60293607-60293626 Msa0261720:CDS 20.0%
!! TGATGCAATAATATTCTTAT+AGG - chr2_2:60293821-60293840 Msa0261720:intron 20.0%
!!! ATAAACAATTTTCAAGAGTT+TGG + chr2_2:60294738-60294757 None:intergenic 20.0%
!! TTGTAATGCTTAAAACAATA+CGG - chr2_2:60294775-60294794 Msa0261720:five_prime_UTR 20.0%
! TCTAGATGTTCATTTAATCT+TGG - chr2_2:60292079-60292098 Msa0261720:CDS 25.0%
! CACACAAATTTAGATATTTG+TGG + chr2_2:60292108-60292127 None:intergenic 25.0%
! ATATGAATGGATAAAGAAAC+CGG + chr2_2:60292421-60292440 None:intergenic 25.0%
! TTATCCATTCATATAGCTTT+CGG - chr2_2:60292427-60292446 Msa0261720:CDS 25.0%
! TTAATACTTTCTATAGTGAG+AGG - chr2_2:60292518-60292537 Msa0261720:CDS 25.0%
! ACTAAATATTCTGTTTGGTA+AGG + chr2_2:60292820-60292839 None:intergenic 25.0%
! CCATTACTAAATATTCTGTT+TGG + chr2_2:60292825-60292844 None:intergenic 25.0%
! CCAAACAGAATATTTAGTAA+TGG - chr2_2:60292822-60292841 Msa0261720:CDS 25.0%
!! TATTTAGTAATGGAAAGAGT+CGG - chr2_2:60292832-60292851 Msa0261720:CDS 25.0%
!! AGTAAGTATGTTTCATGTTT+TGG - chr2_2:60292920-60292939 Msa0261720:CDS 25.0%
! TCAAGAGTAATCTTGTAATT+TGG - chr2_2:60293484-60293503 Msa0261720:CDS 25.0%
! ATTACGGGATTAAAATACTA+CGG + chr2_2:60293511-60293530 None:intergenic 25.0%
! AAAGAAAACATATACGGAAA+AGG + chr2_2:60293558-60293577 None:intergenic 25.0%
!!! CTTATTTTCTTCACTTTTTC+AGG - chr2_2:60293606-60293625 Msa0261720:CDS 25.0%
!! AATTTGTTCATGGGTAATAT+TGG - chr2_2:60293634-60293653 Msa0261720:CDS 25.0%
!! TGAAAGAATTAAAAAGTGCA+AGG - chr2_2:60293850-60293869 Msa0261720:intron 25.0%
!! GAAAGAATTAAAAAGTGCAA+GGG - chr2_2:60293851-60293870 Msa0261720:intron 25.0%
! TAAATATCCAACTTCGAAAA+TGG - chr2_2:60294371-60294390 Msa0261720:five_prime_UTR 25.0%
! TTGAAAATTGTTTATTCAGC+TGG - chr2_2:60294743-60294762 Msa0261720:five_prime_UTR 25.0%
!!! AAACTAGCTCCTTTTTTACT+CGG + chr2_2:60292469-60292488 None:intergenic 30.0%
ACAAGCAATAGAAGTAATGA+CGG - chr2_2:60292696-60292715 Msa0261720:intron 30.0%
!!! AATTTGGACTGTTTTTGTAG+TGG - chr2_2:60293337-60293356 Msa0261720:intron 30.0%
ACTCATAGTTTACTGATAAG+TGG - chr2_2:60293448-60293467 Msa0261720:CDS 30.0%
AACACACACAATTTGATTAC+GGG + chr2_2:60293526-60293545 None:intergenic 30.0%
GAACACACACAATTTGATTA+CGG + chr2_2:60293527-60293546 None:intergenic 30.0%
ATGATTTCATATGACACACT+TGG + chr2_2:60293745-60293764 None:intergenic 30.0%
! AATTAAAAAGTGCAAGGGTA+GGG - chr2_2:60293856-60293875 Msa0261720:intron 30.0%
!!! TTGTGTGTCTATTTTTGGAT+GGG - chr2_2:60294406-60294425 Msa0261720:five_prime_UTR 30.0%
!!! TCTATTTTTGGATGGGAAAA+TGG - chr2_2:60294413-60294432 Msa0261720:five_prime_UTR 30.0%
ACATGTCTCATTATATTCTG+AGG + chr2_2:60294577-60294596 None:intergenic 30.0%
GAATATAATGAGACATGTGT+AGG - chr2_2:60294579-60294598 Msa0261720:five_prime_UTR 30.0%
!! TTCCTAGATTTTCTGAAGAA+CGG - chr2_2:60294712-60294731 Msa0261720:five_prime_UTR 30.0%
! TCTAGCTTCATTCTTAATGT+CGG + chr2_2:60294881-60294900 None:intergenic 30.0%
!!! AGTTTTAATTGCACAGTTCC+CGG - chr2_2:60292154-60292173 Msa0261720:CDS 35.0%
ATGAGGTCAATGTGAATTCT+GGG - chr2_2:60292215-60292234 Msa0261720:CDS 35.0%
TTTGCCGAAAGCTATATGAA+TGG + chr2_2:60292434-60292453 None:intergenic 35.0%
TTCTATAGTGAGAGGATCTT+TGG - chr2_2:60292526-60292545 Msa0261720:CDS 35.0%
TTTACTCAATTGACCAAGCT+TGG - chr2_2:60292560-60292579 Msa0261720:intron 35.0%
!!! TCTTTTTGGGACTGAGAAAA+TGG - chr2_2:60292659-60292678 Msa0261720:intron 35.0%
!!! CTTTTTGGGACTGAGAAAAT+GGG - chr2_2:60292660-60292679 Msa0261720:intron 35.0%
!!! TTTTTGGGACTGAGAAAATG+GGG - chr2_2:60292661-60292680 Msa0261720:intron 35.0%
ACTCCATCTTGTCTTGAAAT+CGG - chr2_2:60292726-60292745 Msa0261720:CDS 35.0%
AATCCGATTTCAAGACAAGA+TGG + chr2_2:60292732-60292751 None:intergenic 35.0%
TTCTTGTATATTTACGCAGC+AGG - chr2_2:60292857-60292876 Msa0261720:CDS 35.0%
TCTTGTATATTTACGCAGCA+GGG - chr2_2:60292858-60292877 Msa0261720:CDS 35.0%
ATCAAGATGCCATGATTGTA+TGG - chr2_2:60292895-60292914 Msa0261720:CDS 35.0%
TCAAGATGCCATGATTGTAT+GGG - chr2_2:60292896-60292915 Msa0261720:CDS 35.0%
ACTTACTTCCCATACAATCA+TGG + chr2_2:60292907-60292926 None:intergenic 35.0%
TCATTGAATGAAGACAGACT+TGG - chr2_2:60292965-60292984 Msa0261720:intron 35.0%
! TGAAAGCAATTTTATCGGCA+AGG + chr2_2:60293134-60293153 None:intergenic 35.0%
CTTCTTGGAAAAAGCTTTAC+TGG + chr2_2:60293191-60293210 None:intergenic 35.0%
!! CAGTAAAGCTTTTTCCAAGA+AGG - chr2_2:60293189-60293208 Msa0261720:intron 35.0%
! TTCAAGCTTTATGTCCTTCT+TGG + chr2_2:60293206-60293225 None:intergenic 35.0%
AAGGACATAAAGCTTGAAAG+TGG - chr2_2:60293208-60293227 Msa0261720:intron 35.0%
TGAGAATAATTTCTCTGCTG+AGG - chr2_2:60293237-60293256 Msa0261720:intron 35.0%
ACGATCAGCATCTATCATAT+TGG - chr2_2:60293396-60293415 Msa0261720:intron 35.0%
TAGTTTACTGATAAGTGGAC+AGG - chr2_2:60293453-60293472 Msa0261720:CDS 35.0%
! TCAGGGTTCAAATTTGTTCA+TGG - chr2_2:60293624-60293643 Msa0261720:CDS 35.0%
! CAGGGTTCAAATTTGTTCAT+GGG - chr2_2:60293625-60293644 Msa0261720:CDS 35.0%
! GAATTAAAAAGTGCAAGGGT+AGG - chr2_2:60293855-60293874 Msa0261720:intron 35.0%
! ATTTGGCGATTTTTGCTTGA+AGG - chr2_2:60293961-60293980 Msa0261720:CDS 35.0%
! GATTTTTGCTTGAAGGAGTA+TGG - chr2_2:60293968-60293987 Msa0261720:CDS 35.0%
CAAGAACAATGAACTGATCT+CGG + chr2_2:60294036-60294055 None:intergenic 35.0%
CTCAAACCTTTGTCTTCTTT+CGG - chr2_2:60294229-60294248 Msa0261720:five_prime_UTR 35.0%
! TGCTGTTGTGTGTCTATTTT+TGG - chr2_2:60294401-60294420 Msa0261720:five_prime_UTR 35.0%
!!! GTTGTGTGTCTATTTTTGGA+TGG - chr2_2:60294405-60294424 Msa0261720:five_prime_UTR 35.0%
TATAATGAGACATGTGTAGG+AGG - chr2_2:60294582-60294601 Msa0261720:five_prime_UTR 35.0%
ACATTGACCTCATCGAAATG+CGG + chr2_2:60292208-60292227 None:intergenic 40.0%
GATGAGGTCAATGTGAATTC+TGG - chr2_2:60292214-60292233 Msa0261720:CDS 40.0%
TGAGGTCAATGTGAATTCTG+GGG - chr2_2:60292216-60292235 Msa0261720:CDS 40.0%
CGCATTTGACCGAGTAAAAA+AGG - chr2_2:60292457-60292476 Msa0261720:CDS 40.0%
AATTCACACCTTGTTGCTGT+TGG + chr2_2:60292492-60292511 None:intergenic 40.0%
!! AAACTCTGAGATTCCAAGCT+TGG + chr2_2:60292576-60292595 None:intergenic 40.0%
CAATAGAAGTAATGACGGCT+CGG - chr2_2:60292701-60292720 Msa0261720:intron 40.0%
TGTCTTGAAATCGGATTGTG+TGG - chr2_2:60292735-60292754 Msa0261720:CDS 40.0%
TGAAGACAGACTTGGAATCT+TGG - chr2_2:60292973-60292992 Msa0261720:intron 40.0%
! TTTTGGATTCATCTGAGTCG+AGG - chr2_2:60293155-60293174 Msa0261720:CDS 40.0%
CATAAAGCTTGAAAGTGGAG+AGG - chr2_2:60293213-60293232 Msa0261720:intron 40.0%
TAATTTCTCTGCTGAGGATG+AGG - chr2_2:60293243-60293262 Msa0261720:intron 40.0%
! AGCTTTCATGAAGGCATACA+AGG - chr2_2:60293285-60293304 Msa0261720:intron 40.0%
GCTGAAGTCTCATGCAAATT+TGG - chr2_2:60293321-60293340 Msa0261720:intron 40.0%
TGGTGATTCAAGAGCAATCA+TGG - chr2_2:60293654-60293673 Msa0261720:CDS 40.0%
GGTGATTCAAGAGCAATCAT+GGG - chr2_2:60293655-60293674 Msa0261720:CDS 40.0%
TATGACACACTTGGCAAATC+AGG + chr2_2:60293736-60293755 None:intergenic 40.0%
AGGATGAACCAGAAGTTTGT+AGG - chr2_2:60293891-60293910 Msa0261720:intron 40.0%
GGATGAACCAGAAGTTTGTA+GGG - chr2_2:60293892-60293911 Msa0261720:intron 40.0%
!!! TTACCTTTTGATGATGCACC+TGG - chr2_2:60293920-60293939 Msa0261720:CDS 40.0%
AATCCAGGTGCATCATCAAA+AGG + chr2_2:60293926-60293945 None:intergenic 40.0%
! TTAGCAATGGCTAGAGCATT+TGG - chr2_2:60293944-60293963 Msa0261720:CDS 40.0%
TCAGTTCATTGTTCTTGCCT+CGG - chr2_2:60294039-60294058 Msa0261720:CDS 40.0%
GAAATTTCTTACACCATCCG+AGG + chr2_2:60294059-60294078 None:intergenic 40.0%
!! GGATGTTTTGAGCAATGAAG+AGG - chr2_2:60294266-60294285 Msa0261720:five_prime_UTR 40.0%
!! TGTTTTGAGCAATGAAGAGG+TGG - chr2_2:60294269-60294288 Msa0261720:five_prime_UTR 40.0%
! TTTAAGTTTCCACTCTCGTG+CGG + chr2_2:60294355-60294374 None:intergenic 40.0%
! CAGTCATCCATTTTCGAAGT+TGG + chr2_2:60294381-60294400 None:intergenic 40.0%
AATCCAGTTGAGTCAGATGA+TGG - chr2_2:60294507-60294526 Msa0261720:five_prime_UTR 40.0%
CATCAGCTGAAGATCAAAAC+TGG - chr2_2:60294646-60294665 Msa0261720:five_prime_UTR 40.0%
GCTGAAGATCAAAACTGGTT+AGG - chr2_2:60294651-60294670 Msa0261720:five_prime_UTR 40.0%
CAAAACTGGTTAGGTCTAGA+AGG - chr2_2:60294660-60294679 Msa0261720:five_prime_UTR 40.0%
TCAAACTCCGCATTTCGATG+AGG - chr2_2:60292198-60292217 Msa0261720:CDS 45.0%
! GTCAATGTGAATTCTGGGGA+AGG - chr2_2:60292220-60292239 Msa0261720:CDS 45.0%
TGTGTCTTCTTCTGTTCAGC+CGG - chr2_2:60292399-60292418 Msa0261720:CDS 45.0%
! GCTAGTTTCCAACAGCAACA+AGG - chr2_2:60292481-60292500 Msa0261720:CDS 45.0%
!! GTGAGAGGATCTTTGGAATC+AGG - chr2_2:60292533-60292552 Msa0261720:CDS 45.0%
TCAGAAGATGCGATCTTCTG+CGG - chr2_2:60293052-60293071 Msa0261720:intron 45.0%
ACCTAGTAGCACGCAAAGTT+AGG - chr2_2:60293104-60293123 Msa0261720:CDS 45.0%
CCTAGTAGCACGCAAAGTTA+GGG - chr2_2:60293105-60293124 Msa0261720:CDS 45.0%
AGGATGAGGTGAGTTCTATG+TGG - chr2_2:60293257-60293276 Msa0261720:intron 45.0%
ATGAGGTGAGTTCTATGTGG+AGG - chr2_2:60293260-60293279 Msa0261720:intron 45.0%
TGAGGTGAGTTCTATGTGGA+GGG - chr2_2:60293261-60293280 Msa0261720:intron 45.0%
AGACTTCAGCTCTTTGTCCA+TGG + chr2_2:60293311-60293330 None:intergenic 45.0%
ATCTGTGACTGTAGTGAAGC+AGG - chr2_2:60293366-60293385 Msa0261720:intron 45.0%
AAGAGCAATCATGGGATCCA+AGG - chr2_2:60293663-60293682 Msa0261720:CDS 45.0%
CATCAAGTCATTGCTGTCCT+TGG + chr2_2:60293683-60293702 None:intergenic 45.0%
CAAGGACAGCAATGACTTGA+TGG - chr2_2:60293681-60293700 Msa0261720:CDS 45.0%
! CAAGGGTAGGGTATTTGCAT+TGG - chr2_2:60293868-60293887 Msa0261720:intron 45.0%
AACCAGAAGTTTGTAGGGTG+TGG - chr2_2:60293897-60293916 Msa0261720:CDS 45.0%
AACCACACCCTACAAACTTC+TGG + chr2_2:60293902-60293921 None:intergenic 45.0%
!! TGATGCACCTGGATTAGCAA+TGG - chr2_2:60293931-60293950 Msa0261720:CDS 45.0%
CTGTACCTGAATTTACGCAC+CGG - chr2_2:60293999-60294018 Msa0261720:CDS 45.0%
TTCATTGTTCTTGCCTCGGA+TGG - chr2_2:60294043-60294062 Msa0261720:CDS 45.0%
CAGAGACCGAAAGAAGACAA+AGG + chr2_2:60294238-60294257 None:intergenic 45.0%
TCTTTCGGTCTCTGCAGATA+TGG - chr2_2:60294244-60294263 Msa0261720:five_prime_UTR 45.0%
CTTTCGGTCTCTGCAGATAT+GGG - chr2_2:60294245-60294264 Msa0261720:five_prime_UTR 45.0%
ATCAGCACCAAGTCGATCAT+TGG - chr2_2:60294305-60294324 Msa0261720:five_prime_UTR 45.0%
!! TGAATGCCTGTTGCTTTGGA+TGG + chr2_2:60294484-60294503 None:intergenic 45.0%
ATCCAAAGCAACAGGCATTC+AGG - chr2_2:60294483-60294502 Msa0261720:five_prime_UTR 45.0%
TGACCATCATCTGACTCAAC+TGG + chr2_2:60294513-60294532 None:intergenic 45.0%
GTGAAGTTCCTTCGCAAAGA+TGG + chr2_2:60294546-60294565 None:intergenic 45.0%
AATGAGACATGTGTAGGAGG+AGG - chr2_2:60294585-60294604 Msa0261720:five_prime_UTR 45.0%
ACATGTGTAGGAGGAGGAAA+AGG - chr2_2:60294591-60294610 Msa0261720:five_prime_UTR 45.0%
GAGTCTGTCGATGTTGAAGA+CGG - chr2_2:60294618-60294637 Msa0261720:five_prime_UTR 45.0%
CTGTACCAGTGAGTTTACTC+GGG + chr2_2:60294691-60294710 None:intergenic 45.0%
GCTGTACCAGTGAGTTTACT+CGG + chr2_2:60294692-60294711 None:intergenic 45.0%
GGCCGTTCTTCAGAAAATCT+AGG + chr2_2:60294717-60294736 None:intergenic 45.0%
ATTTACGCAGCAGGGTCGTA+AGG - chr2_2:60292866-60292885 Msa0261720:CDS 50.0%
TTTACGCAGCAGGGTCGTAA+GGG - chr2_2:60292867-60292886 Msa0261720:CDS 50.0%
ATCTTCTGCGGTGTGTTCGA+TGG - chr2_2:60293064-60293083 Msa0261720:intron 50.0%
TTCGATGGCCATGGTCCATA+CGG - chr2_2:60293079-60293098 Msa0261720:CDS 50.0%
! CCCTAACTTTGCGTGCTACT+AGG + chr2_2:60293108-60293127 None:intergenic 50.0%
GTGGAGGGAAGCTTTCATGA+AGG - chr2_2:60293276-60293295 Msa0261720:intron 50.0%
! CATGAAGGCATACAAGGCCA+TGG - chr2_2:60293291-60293310 Msa0261720:intron 50.0%
! GGGTAGGGTATTTGCATTGG+AGG - chr2_2:60293871-60293890 Msa0261720:intron 50.0%
GCTCTAGCCATTGCTAATCC+AGG + chr2_2:60293941-60293960 None:intergenic 50.0%
AGAAGCCGGTGCGTAAATTC+AGG + chr2_2:60294007-60294026 None:intergenic 50.0%
AAGTCGATCATTGGCAGCGA+GGG - chr2_2:60294314-60294333 Msa0261720:five_prime_UTR 50.0%
!! CCTGTTGCTTTGGATGGTTG+CGG + chr2_2:60294478-60294497 None:intergenic 50.0%
! TGCCTGAATGCCTGTTGCTT+TGG + chr2_2:60294488-60294507 None:intergenic 50.0%
GTTCCTTCGCAAAGATGGCT+CGG + chr2_2:60294541-60294560 None:intergenic 50.0%
TGTGTAGGAGGAGGAAAAGG+AGG - chr2_2:60294594-60294613 Msa0261720:five_prime_UTR 50.0%
TGTCACCCGAGTAAACTCAC+TGG - chr2_2:60294683-60294702 Msa0261720:five_prime_UTR 50.0%
CTAGGTGACCGTATGGACCA+TGG + chr2_2:60293090-60293109 None:intergenic 55.0%
! CGTGCTACTAGGTGACCGTA+TGG + chr2_2:60293097-60293116 None:intergenic 55.0%
GATCTCGGTCTGTGAGAAGC+CGG + chr2_2:60294021-60294040 None:intergenic 55.0%
CTCGCTGCCAATGATCGACT+TGG + chr2_2:60294315-60294334 None:intergenic 55.0%
CAAGTCGATCATTGGCAGCG+AGG - chr2_2:60294313-60294332 Msa0261720:five_prime_UTR 55.0%
ATCATTGGCAGCGAGGGTTC+TGG - chr2_2:60294320-60294339 Msa0261720:five_prime_UTR 55.0%
CCGCAACCATCCAAAGCAAC+AGG - chr2_2:60294475-60294494 Msa0261720:five_prime_UTR 55.0%
AGTCCGAGCCATCTTTGCGA+AGG - chr2_2:60294535-60294554 Msa0261720:five_prime_UTR 55.0%
TGCGGTGTGTTCGATGGCCA+TGG - chr2_2:60293070-60293089 Msa0261720:CDS 60.0%
! ATTGGCAGCGAGGGTTCTGG+TGG - chr2_2:60294323-60294342 Msa0261720:five_prime_UTR 60.0%
TGGAATCTGCCGCACGAGAG+TGG - chr2_2:60294343-60294362 Msa0261720:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 60291909 60294904 60291909 ID=Msa0261720;Name=Msa0261720
chr2_2 mRNA 60291909 60294904 60291909 ID=Msa0261720-mRNA-1;Parent=Msa0261720;Name=Msa0261720-mRNA-1;_AED=0.15;_eAED=0.15;_QI=736|0.8|0.83|1|0.8|0.66|6|158|480
chr2_2 exon 60291909 60292552 60291909 ID=Msa0261720-mRNA-1:exon:18568;Parent=Msa0261720-mRNA-1
chr2_2 exon 60292720 60292970 60292720 ID=Msa0261720-mRNA-1:exon:18567;Parent=Msa0261720-mRNA-1
chr2_2 exon 60293068 60293185 60293068 ID=Msa0261720-mRNA-1:exon:18566;Parent=Msa0261720-mRNA-1
chr2_2 exon 60293426 60293770 60293426 ID=Msa0261720-mRNA-1:exon:18565;Parent=Msa0261720-mRNA-1
chr2_2 exon 60293893 60294803 60293893 ID=Msa0261720-mRNA-1:exon:18564;Parent=Msa0261720-mRNA-1
chr2_2 exon 60294837 60294904 60294837 ID=Msa0261720-mRNA-1:exon:18563;Parent=Msa0261720-mRNA-1
chr2_2 five_prime_UTR 60294837 60294904 60294837 ID=Msa0261720-mRNA-1:five_prime_utr;Parent=Msa0261720-mRNA-1
chr2_2 five_prime_UTR 60294136 60294803 60294136 ID=Msa0261720-mRNA-1:five_prime_utr;Parent=Msa0261720-mRNA-1
chr2_2 CDS 60293893 60294135 60293893 ID=Msa0261720-mRNA-1:cds;Parent=Msa0261720-mRNA-1
chr2_2 CDS 60293426 60293770 60293426 ID=Msa0261720-mRNA-1:cds;Parent=Msa0261720-mRNA-1
chr2_2 CDS 60293068 60293185 60293068 ID=Msa0261720-mRNA-1:cds;Parent=Msa0261720-mRNA-1
chr2_2 CDS 60292720 60292970 60292720 ID=Msa0261720-mRNA-1:cds;Parent=Msa0261720-mRNA-1
chr2_2 CDS 60292067 60292552 60292067 ID=Msa0261720-mRNA-1:cds;Parent=Msa0261720-mRNA-1
chr2_2 three_prime_UTR 60291909 60292066 60291909 ID=Msa0261720-mRNA-1:three_prime_utr;Parent=Msa0261720-mRNA-1
Gene Sequence

>Msa0261720

ATGGGGTGTTGTGTCTCAACAAGCAATAGAAGTAATGACGGCTCGGTTACTCCATCTTGTCTTGAAATCGGATTGTGTGGTCAAAAGAAAGCAAGAAGAACATTCTCTGATCATGTTGTCTCTCTGAACAAGTTAGCATCCTTACCAAACAGAATATTTAGTAATGGAAAGAGTCGGTCTTCTTGTATATTTACGCAGCAGGGTCGTAAGGGTGTTAATCAAGATGCCATGATTGTATGGGAAGATTTTGTGTCAGAAGATGCGATCTTCTGCGGTGTGTTCGATGGCCATGGTCCATACGGTCACCTAGTAGCACGCAAAGTTAGGGATGCCTTGCCGATAAAATTGCTTTCATTTTTGGATTCATCTGAGTCGAGGCAAAATGAATCCAGTAAAGCTTTTTCCAAGAAGGACATAAAGCTTGAAAGTGGAGAGGCTGAGAATAATTTCTCTGCTGAGGATGAGGTGAGTTCTATGTGGAGGGAAGCTTTCATGAAGGCATACAAGGCCATGGACAAAGAGCTGAAGTCTCATGCAAATTTGGACTGTTTTTGTAGTGGAAGCACATCTGTGACTGTAGTGAAGCAGGGTTCAAATTTGTTCATGGGTAATATTGGTGATTCAAGAGCAATCATGGGATCCAAGGACAGCAATGACTTGATGGTTGCAATTCAGCTGACAGTTGATTTAAAACCTGATTTGCCAAGAGAAGCTGAAAGAATTAAAAAGTGCAAGGGTAGGGTATTTGCATTGGAGGATGAACCAGAAGTTTGTAGGGTGTGGTTACCTTTTGATGATGCACCTGGATTAGCAATGGCTAGAGCATTTGGCGATTTTTGCTTGAAGGAGTATGGTGTGATTTCTGTACCTGAATTTACGCACCGGCTTCTCACAGACCGAGATCAGTTCATTGTTCTTGCCTCGGATGGTGTAAGAAATTTCAATTTCATATCTACTATATGGGATGTTTTGAGCAATGAAGAGGTGGTAGAAATAGTATCATCAGCACCAAGTCGATCATTGGCAGCGAGGGTTCTGGTGGAATCTGCCGCACGAGAGTGGAAACTTAAATATCCAACTTCGAAAATGGATGACTGTGCTGTTGTGTGTCTATTTTTGGATGGGAAAATGGACTCAGAAGAATCTGATTGTGATGAACAATGCTATTCTTCCGCAACCATCCAAAGCAACAGGCATTCAGGCAATCCAGTTGAGTCAGATGATGGTCAAAAGTCCGAGCCATCTTTGCGAAGGAACTTCACTGTGAGAACCTCAGAATATAATGAGACATGTGTAGGAGGAGGAAAAGGAGGAGAGTCTGTCGATGTTGAAGACGGAACATCATCAGCTGAAGATCAAAACTGGTTAGGTCTAGAAGGTGTCACCCGAGTAAACTCACTGGTACAGCTTCCTAGATTTTCTGAAGAACGGCCAAACTCTTGA
Protein sequence

>Msa0261720

MGCCVSTSNRSNDGSVTPSCLEIGLCGQKKARRTFSDHVVSLNKLASLPNRIFSNGKSRSSCIFTQQGRKGVNQDAMIVWEDFVSEDAIFCGVFDGHGPYGHLVARKVRDALPIKLLSFLDSSESRQNESSKAFSKKDIKLESGEAENNFSAEDEVSSMWREAFMKAYKAMDKELKSHANLDCFCSGSTSVTVVKQGSNLFMGNIGDSRAIMGSKDSNDLMVAIQLTVDLKPDLPREAERIKKCKGRVFALEDEPEVCRVWLPFDDAPGLAMARAFGDFCLKEYGVISVPEFTHRLLTDRDQFIVLASDGVRNFNFISTIWDVLSNEEVVEIVSSAPSRSLAARVLVESAAREWKLKYPTSKMDDCAVVCLFLDGKMDSEESDCDEQCYSSATIQSNRHSGNPVESDDGQKSEPSLRRNFTVRTSEYNETCVGGGKGGESVDVEDGTSSAEDQNWLGLEGVTRVNSLVQLPRFSEERPNS*