Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0006110 | Msa1110090 | 0.806586 | 7.653756e-50 | -8.615850e-47 |
| Msa0056450 | Msa1110090 | 0.800373 | 1.485904e-48 | -8.615850e-47 |
| Msa0058160 | Msa1110090 | 0.813261 | 2.799572e-51 | -8.615850e-47 |
| Msa0106710 | Msa1110090 | 0.808620 | 2.831929e-50 | -8.615850e-47 |
| Msa0659210 | Msa1110090 | 0.810858 | 9.353474e-51 | -8.615850e-47 |
| Msa0661320 | Msa1110090 | 0.818867 | 1.566474e-52 | -8.615850e-47 |
| Msa0701010 | Msa1110090 | 0.828739 | 7.602636e-55 | -8.615850e-47 |
| Msa0705190 | Msa1110090 | 0.838007 | 3.712880e-57 | -8.615850e-47 |
| Msa0731940 | Msa1110090 | 0.807814 | 4.206094e-50 | -8.615850e-47 |
| Msa0748970 | Msa1110090 | 0.809462 | 1.870223e-50 | -8.615850e-47 |
| Msa0760130 | Msa1110090 | 0.801036 | 1.088267e-48 | -8.615850e-47 |
| Msa0768290 | Msa1110090 | 0.854169 | 1.472366e-61 | -8.615850e-47 |
| Msa0768370 | Msa1110090 | 0.819960 | 8.826620e-53 | -8.615850e-47 |
| Msa0773180 | Msa1110090 | 0.812464 | 4.184034e-51 | -8.615850e-47 |
| Msa0810220 | Msa1110090 | 0.800791 | 1.221139e-48 | -8.615850e-47 |
| Msa0135660 | Msa1110090 | 0.808330 | 3.266374e-50 | -8.615850e-47 |
| Msa0145190 | Msa1110090 | 0.800473 | 1.417479e-48 | -8.615850e-47 |
| Msa0220730 | Msa1110090 | 0.806199 | 9.237764e-50 | -8.615850e-47 |
| Msa0384890 | Msa1110090 | 0.807378 | 5.205337e-50 | -8.615850e-47 |
| Msa0394140 | Msa1110090 | 0.814885 | 1.226418e-51 | -8.615850e-47 |
| Msa0394260 | Msa1110090 | 0.813235 | 2.836825e-51 | -8.615850e-47 |
| Msa0413410 | Msa1110090 | 0.807110 | 5.931955e-50 | -8.615850e-47 |
| Msa0420590 | Msa1110090 | -0.816130 | 6.479687e-52 | -8.615850e-47 |
| Msa0420650 | Msa1110090 | -0.816453 | 5.488297e-52 | -8.615850e-47 |
| Msa0424320 | Msa1110090 | 0.811791 | 5.867359e-51 | -8.615850e-47 |
| Msa0471550 | Msa1110090 | 0.807535 | 4.821104e-50 | -8.615850e-47 |
| Msa0479340 | Msa1110090 | 0.819490 | 1.130087e-52 | -8.615850e-47 |
| Msa1000880 | Msa1110090 | 0.802111 | 6.551137e-49 | -8.615850e-47 |
| Msa1002810 | Msa1110090 | 0.803418 | 3.518721e-49 | -8.615850e-47 |
| Msa1012590 | Msa1110090 | -0.810384 | 1.183927e-50 | -8.615850e-47 |
| Msa1015490 | Msa1110090 | 0.818360 | 2.041507e-52 | -8.615850e-47 |
| Msa1050740 | Msa1110090 | 0.814872 | 1.235040e-51 | -8.615850e-47 |
| Msa1080960 | Msa1110090 | 0.814034 | 1.891823e-51 | -8.615850e-47 |
| Msa1098240 | Msa1110090 | 0.812463 | 4.186073e-51 | -8.615850e-47 |
| Msa1098250 | Msa1110090 | 0.809759 | 1.614619e-50 | -8.615850e-47 |
| Msa0241190 | Msa1110090 | 0.823897 | 1.081203e-53 | -8.615850e-47 |
| Msa0278190 | Msa1110090 | 0.803270 | 3.776170e-49 | -8.615850e-47 |
| Msa0321310 | Msa1110090 | 0.830700 | 2.533386e-55 | -8.615850e-47 |
| Msa1110090 | Msa1121480 | 0.840369 | 9.066479e-58 | -8.615850e-47 |
| Msa1110090 | Msa1121490 | 0.837247 | 5.815685e-57 | -8.615850e-47 |
| Msa1110090 | Msa1137570 | 0.822869 | 1.880069e-53 | -8.615850e-47 |
| Msa1110090 | Msa1195180 | 0.810022 | 1.416831e-50 | -8.615850e-47 |
| Msa1110090 | Msa1248310 | 0.801792 | 7.616457e-49 | -8.615850e-47 |
| Msa1110090 | Msa1353470 | 0.814882 | 1.228254e-51 | -8.615850e-47 |
| Msa1110090 | Msa1408010 | 0.800659 | 1.299175e-48 | -8.615850e-47 |
| Msa1110090 | Msa1443060 | 0.803683 | 3.101298e-49 | -8.615850e-47 |
| Msa0523940 | Msa1110090 | 0.813764 | 2.169580e-51 | -8.615850e-47 |
| Msa0548150 | Msa1110090 | 0.827602 | 1.428544e-54 | -8.615850e-47 |
| Msa0844490 | Msa1110090 | 0.807840 | 4.151924e-50 | -8.615850e-47 |
| Msa0868160 | Msa1110090 | 0.821687 | 3.537339e-53 | -8.615850e-47 |
| Msa0877250 | Msa1110090 | 0.812321 | 4.495895e-51 | -8.615850e-47 |
| Msa0899430 | Msa1110090 | 0.819142 | 1.356256e-52 | -8.615850e-47 |
| Msa0899450 | Msa1110090 | 0.844642 | 6.663494e-59 | -8.615850e-47 |
| Msa0925870 | Msa1110090 | 0.815464 | 9.124028e-52 | -8.615850e-47 |
| Msa0936390 | Msa1110090 | 0.805000 | 1.648888e-49 | -8.615850e-47 |
| Msa0958550 | Msa1110090 | 0.814740 | 1.320913e-51 | -8.615850e-47 |
| Msa0964900 | Msa1110090 | 0.801719 | 7.884699e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATCTCATCAAGTATTAAAA+AGG | 0.260384 | 7_3:-54699029 | None:intergenic |
| CATGAGTACAAAATAGAAAT+TGG | 0.324835 | 7_3:-54699069 | None:intergenic |
| AAACCAGATGTTTGTGAAGA+TGG | 0.418965 | 7_3:+54699133 | Msa1110090:CDS |
| CAACCATCTTCACAAACATC+TGG | 0.441838 | 7_3:-54699136 | None:intergenic |
| TTCCTTCTCTGAGGTTAATG+AGG | 0.457881 | 7_3:+54698985 | Msa1110090:CDS |
| ACTCATGTGCCTGAGGTAGC+TGG | 0.474913 | 7_3:+54699085 | Msa1110090:CDS |
| CCTGAGGTAGCTGGAAATCT+CGG | 0.544146 | 7_3:+54699094 | Msa1110090:CDS |
| CCGAGATTTCCAGCTACCTC+AGG | 0.573189 | 7_3:-54699094 | None:intergenic |
| AACCTCATTAACCTCAGAGA+AGG | 0.600801 | 7_3:-54698987 | None:intergenic |
| GAGTACAAAATAGAAATTGG+TGG | 0.618243 | 7_3:-54699066 | None:intergenic |
| AGTTAAGAGTTCCTTCTCTG+AGG | 0.621922 | 7_3:+54698976 | Msa1110090:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATCTCATCAAGTATTAAAA+AGG | - | chr7_3:54699032-54699051 | None:intergenic | 20.0% |
| !!! | TTTTTAATACTTGATGAGAT+TGG | + | chr7_3:54699031-54699050 | Msa1110090:CDS | 20.0% |
| ! | CATGAGTACAAAATAGAAAT+TGG | - | chr7_3:54699072-54699091 | None:intergenic | 25.0% |
| GAGTACAAAATAGAAATTGG+TGG | - | chr7_3:54699069-54699088 | None:intergenic | 30.0% | |
| AAACCAGATGTTTGTGAAGA+TGG | + | chr7_3:54699133-54699152 | Msa1110090:CDS | 35.0% | |
| AGTTAAGAGTTCCTTCTCTG+AGG | + | chr7_3:54698976-54698995 | Msa1110090:CDS | 40.0% | |
| !! | TTCCTTCTCTGAGGTTAATG+AGG | + | chr7_3:54698985-54699004 | Msa1110090:CDS | 40.0% |
| AACCTCATTAACCTCAGAGA+AGG | - | chr7_3:54698990-54699009 | None:intergenic | 40.0% | |
| ! | ATTTTGTACTCATGTGCCTG+AGG | + | chr7_3:54699078-54699097 | Msa1110090:CDS | 40.0% |
| CAACCATCTTCACAAACATC+TGG | - | chr7_3:54699139-54699158 | None:intergenic | 40.0% | |
| CCTGAGGTAGCTGGAAATCT+CGG | + | chr7_3:54699094-54699113 | Msa1110090:CDS | 50.0% | |
| ACTCATGTGCCTGAGGTAGC+TGG | + | chr7_3:54699085-54699104 | Msa1110090:CDS | 55.0% | |
| CCGAGATTTCCAGCTACCTC+AGG | - | chr7_3:54699097-54699116 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_3 | gene | 54698965 | 54699159 | 54698965 | ID=Msa1110090;Name=Msa1110090 |
| chr7_3 | mRNA | 54698965 | 54699159 | 54698965 | ID=Msa1110090-mRNA-1;Parent=Msa1110090;Name=Msa1110090-mRNA-1;_AED=0.45;_eAED=1.00;_QI=0|-1|0|1|-1|1|1|0|64 |
| chr7_3 | exon | 54698965 | 54699159 | 54698965 | ID=Msa1110090-mRNA-1:exon:11538;Parent=Msa1110090-mRNA-1 |
| chr7_3 | CDS | 54698965 | 54699159 | 54698965 | ID=Msa1110090-mRNA-1:cds;Parent=Msa1110090-mRNA-1 |
| Gene Sequence |
| Protein sequence |
