AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0705190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0705190 MtrunA17_Chr8g0385241 63.170 429 73 5 55 399 4 431 6.25e-169 479
Msa0705190 MtrunA17_Chr8g0382971 68.156 358 104 4 42 399 6 353 2.50e-165 467
Msa0705190 MtrunA17_Chr8g0383551 56.364 385 116 8 44 399 6 367 3.21e-125 366
Msa0705190 MtrunA17_Chr4g0059911 65.979 291 89 5 55 343 3 285 9.56e-124 362
Msa0705190 MtrunA17_Chr8g0377671 59.449 254 102 1 61 314 19 271 6.12e-97 291
Msa0705190 MtrunA17_Chr8g0377671 50.431 232 91 2 108 316 20 250 1.82e-65 210
Msa0705190 MtrunA17_Chr8g0377671 47.879 165 85 1 56 220 106 269 2.19e-40 145
Msa0705190 MtrunA17_Chr8g0383331 78.049 164 34 2 152 314 2 164 1.77e-82 249
Msa0705190 MtrunA17_Chr8g0383331 44.156 154 84 2 61 213 3 155 3.27e-35 127
Msa0705190 MtrunA17_Chr8g0377191 61.275 204 77 2 53 256 4 205 9.48e-77 236
Msa0705190 MtrunA17_Chr8g0377191 53.093 194 90 1 109 302 13 205 3.95e-60 193
Msa0705190 MtrunA17_Chr8g0377191 40.650 123 71 1 202 324 13 133 1.77e-19 86.3
Msa0705190 MtrunA17_Chr8g0377191 62.500 48 18 0 269 316 11 58 3.07e-11 62.4
Msa0705190 MtrunA17_Chr8g0380791 43.429 350 166 10 52 388 4 334 4.17e-75 248
Msa0705190 MtrunA17_Chr2g0316611 47.703 283 142 3 42 324 17 293 1.83e-74 236
Msa0705190 MtrunA17_Chr2g0316611 37.037 270 123 3 62 285 105 373 1.22e-46 164
Msa0705190 MtrunA17_Chr2g0316611 39.437 213 104 3 49 237 160 371 6.17e-38 140
Msa0705190 MtrunA17_Chr2g0316631 46.996 283 144 3 42 324 17 293 1.54e-72 229
Msa0705190 MtrunA17_Chr2g0316631 49.600 125 63 0 62 186 174 298 1.20e-32 124
Msa0705190 MtrunA17_Chr2g0316631 59.740 77 30 1 48 123 228 304 2.19e-20 90.9
Msa0705190 MtrunA17_Chr6g0467061 45.985 274 146 2 43 316 1 272 6.47e-69 221
Msa0705190 MtrunA17_Chr6g0467061 40.299 201 96 1 59 259 154 330 2.26e-35 132
Msa0705190 MtrunA17_Chr6g0467061 35.149 202 106 3 153 330 18 218 3.91e-28 113
Msa0705190 MtrunA17_Chr6g0467061 80.488 41 8 0 62 102 295 335 1.45e-13 71.2
Msa0705190 MtrunA17_Chr8g0377211 50.602 166 56 4 55 220 14 153 6.53e-45 153
Msa0705190 MtrunA17_Chr8g0377211 52.143 140 66 1 175 314 17 155 3.69e-39 138
Msa0705190 MtrunA17_Chr8g0377211 44.203 138 52 3 152 266 18 153 1.76e-26 104
Msa0705190 MtrunA17_Chr8g0377211 55.102 49 22 0 58 106 107 155 1.63e-11 62.4
Msa0705190 MtrunA17_Chr8g0377221 39.732 224 88 4 58 239 1 219 2.82e-42 149
Msa0705190 MtrunA17_Chr8g0377221 48.299 147 61 2 62 193 74 220 4.25e-34 127
Msa0705190 MtrunA17_Chr8g0377221 42.017 119 69 0 197 315 1 119 5.70e-23 97.4
Msa0705190 MtrunA17_Chr8g0377221 27.234 235 155 3 104 323 1 234 6.38e-20 89.0
Msa0705190 MtrunA17_Chr8g0353121 42.487 193 108 3 62 253 53 243 9.77e-41 153
Msa0705190 MtrunA17_Chr8g0353121 37.791 172 106 1 106 277 51 221 7.82e-32 127
Msa0705190 MtrunA17_Chr8g0353121 34.595 185 116 2 155 339 54 233 7.35e-22 97.8
Msa0705190 MtrunA17_Chr3g0082501 42.541 181 103 1 62 242 64 243 1.27e-40 153
Msa0705190 MtrunA17_Chr3g0082501 33.803 213 129 4 152 358 62 268 1.67e-23 102
Msa0705190 MtrunA17_Chr3g0082501 27.574 272 160 5 128 376 61 318 2.86e-22 99.4
Msa0705190 MtrunA17_Chr3g0082501 34.513 113 74 0 62 174 133 245 2.29e-12 68.9
Msa0705190 MtrunA17_Chr1g0180261 31.461 356 220 8 47 381 37 389 2.71e-39 149
Msa0705190 MtrunA17_Chr1g0171491 39.894 188 112 1 62 249 61 247 3.18e-35 137
Msa0705190 MtrunA17_Chr1g0171491 29.389 262 171 6 134 389 37 290 6.54e-20 92.0
Msa0705190 MtrunA17_Chr1g0171491 44.444 90 50 0 61 150 152 241 6.74e-15 76.6
Msa0705190 MtrunA17_Chr1g0171491 31.013 158 73 2 62 206 130 264 1.92e-14 75.1
Msa0705190 MtrunA17_Chr5g0424111 34.733 262 154 6 29 277 108 365 1.86e-34 132
Msa0705190 MtrunA17_Chr5g0424111 38.418 177 108 1 57 233 192 367 6.53e-28 114
Msa0705190 MtrunA17_Chr5g0424111 36.723 177 109 2 145 321 188 361 7.93e-28 114
Msa0705190 MtrunA17_Chr5g0424111 34.043 141 91 1 191 331 187 325 1.35e-16 81.3
Msa0705190 MtrunA17_Chr3g0115111 42.529 174 98 2 60 232 55 227 4.79e-34 134
Msa0705190 MtrunA17_Chr3g0115111 30.392 204 134 4 135 337 40 236 5.30e-18 86.3
Msa0705190 MtrunA17_Chr5g0398011 53.097 113 53 0 60 172 33 145 7.18e-33 120
Msa0705190 MtrunA17_Chr5g0398011 41.176 119 70 0 198 316 32 150 1.99e-20 87.0
Msa0705190 MtrunA17_Chr5g0398011 73.333 45 12 0 62 106 104 148 1.22e-15 73.9
Msa0705190 MtrunA17_Chr5g0398011 29.371 143 77 1 105 247 32 150 8.70e-11 60.1
Msa0705190 MtrunA17_Chr7g0233351 37.097 186 114 2 101 284 183 367 1.72e-32 127
Msa0705190 MtrunA17_Chr7g0233351 35.323 201 106 1 61 261 191 367 8.85e-30 119
Msa0705190 MtrunA17_Chr7g0233351 37.017 181 109 3 141 319 177 354 1.42e-27 113
Msa0705190 MtrunA17_Chr7g0233351 34.234 111 73 0 58 168 257 367 8.04e-13 69.7
Msa0705190 MtrunA17_Chr8g0336161 36.464 181 114 1 104 284 193 372 2.26e-32 127
Msa0705190 MtrunA17_Chr8g0336161 33.945 218 125 3 37 239 160 373 3.78e-27 112
Msa0705190 MtrunA17_Chr8g0336161 38.095 168 101 2 152 319 195 359 4.36e-26 109
Msa0705190 MtrunA17_Chr3g0131001 43.258 178 100 1 60 237 53 229 9.84e-32 127
Msa0705190 MtrunA17_Chr3g0131001 32.558 172 111 2 154 325 55 221 3.83e-14 74.3
Msa0705190 MtrunA17_Chr5g0424301 41.212 165 96 1 62 226 69 232 2.48e-31 126
Msa0705190 MtrunA17_Chr5g0424301 34.132 167 109 1 106 272 67 232 3.97e-26 110
Msa0705190 MtrunA17_Chr5g0424301 31.250 160 109 1 154 313 69 227 3.84e-17 83.6
Msa0705190 MtrunA17_Chr4g0055371 34.653 202 122 2 88 289 161 352 6.98e-31 122
Msa0705190 MtrunA17_Chr4g0055371 33.831 201 109 2 61 261 171 347 4.39e-27 111
Msa0705190 MtrunA17_Chr4g0055371 35.393 178 112 2 142 319 160 334 3.46e-26 109
Msa0705190 MtrunA17_Chr4g0055371 31.343 134 92 0 62 195 218 351 2.58e-15 77.4
Msa0705190 MtrunA17_Chr4g0055371 33.333 135 88 1 187 321 158 290 3.01e-15 77.0
Msa0705190 MtrunA17_Chr4g0055371 34.234 111 73 0 58 168 237 347 2.48e-12 68.2
Msa0705190 MtrunA17_Chr3g0114791 35.227 176 113 1 102 277 179 353 1.65e-30 121
Msa0705190 MtrunA17_Chr3g0114791 36.313 179 113 1 60 238 183 360 2.88e-28 115
Msa0705190 MtrunA17_Chr3g0114791 33.889 180 116 2 142 321 173 349 2.68e-25 106
Msa0705190 MtrunA17_Chr3g0114791 33.582 134 87 1 188 321 172 303 2.15e-14 74.3
Msa0705190 MtrunA17_Chr8g0385201 41.304 184 102 4 62 241 30 211 3.78e-30 122
Msa0705190 MtrunA17_Chr8g0385201 38.000 150 91 2 59 208 51 198 5.45e-23 101
Msa0705190 MtrunA17_Chr8g0385201 31.000 200 135 3 150 347 26 224 3.36e-19 89.7
Msa0705190 MtrunA17_Chr7g0256301 40.116 172 102 1 62 233 71 241 4.24e-30 122
Msa0705190 MtrunA17_Chr7g0256301 35.632 174 111 1 106 279 69 241 1.75e-27 114
Msa0705190 MtrunA17_Chr7g0256301 30.247 162 112 1 154 315 71 231 1.14e-16 82.0
Msa0705190 MtrunA17_Chr5g0398021 52.885 104 49 0 62 165 13 116 1.41e-29 112
Msa0705190 MtrunA17_Chr5g0398021 36.975 119 75 0 198 316 10 128 6.71e-20 85.9
Msa0705190 MtrunA17_Chr5g0398021 35.185 108 69 1 105 212 10 116 7.58e-15 71.6
Msa0705190 MtrunA17_Chr8g0377201 66.216 74 25 0 244 317 1 74 8.64e-26 99.8
Msa0705190 MtrunA17_Chr8g0377201 58.065 62 26 0 198 259 1 62 1.52e-17 77.0
Msa0705190 MtrunA17_Chr8g0377201 58.462 65 26 1 153 217 3 66 1.07e-15 72.0
Msa0705190 MtrunA17_Chr8g0377201 60.784 51 20 0 58 108 23 73 7.51e-15 69.7
Msa0705190 MtrunA17_Chr3g0085921 73.333 60 16 0 193 252 2 61 4.11e-23 93.2
Msa0705190 MtrunA17_Chr3g0085921 63.934 61 21 1 146 206 2 61 7.50e-19 81.6
Msa0705190 MtrunA17_Chr3g0085921 34.906 106 46 1 238 343 1 83 2.04e-11 60.8
Msa0705190 MtrunA17_Chr1g0210831 34.146 123 81 0 64 186 615 737 4.46e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0705190 AT1G60890.2 36.127 346 185 7 61 388 22 349 6.99e-57 199
Msa0705190 AT1G60890.1 36.127 346 185 7 61 388 10 337 7.64e-57 199
Msa0705190 AT1G10900.2 36.637 333 188 6 61 388 10 324 5.46e-51 182
Msa0705190 AT1G10900.2 31.410 156 106 1 149 304 6 160 6.65e-16 80.1
Msa0705190 AT1G10900.2 29.730 148 81 2 191 315 1 148 3.66e-11 65.5
Msa0705190 AT1G10900.1 36.637 333 188 6 61 388 10 324 5.46e-51 182
Msa0705190 AT1G10900.1 31.410 156 106 1 149 304 6 160 6.65e-16 80.1
Msa0705190 AT1G10900.1 29.730 148 81 2 191 315 1 148 3.66e-11 65.5
Msa0705190 AT1G10900.3 36.637 333 188 6 61 388 10 324 5.46e-51 182
Msa0705190 AT1G10900.3 31.410 156 106 1 149 304 6 160 6.65e-16 80.1
Msa0705190 AT1G10900.3 29.730 148 81 2 191 315 1 148 3.66e-11 65.5
Msa0705190 AT1G21980.1 40.594 202 110 2 62 263 76 267 5.78e-38 145
Msa0705190 AT1G21980.1 34.104 173 109 2 154 326 76 243 6.25e-22 98.6
Msa0705190 AT1G21980.1 43.750 80 45 0 61 140 167 246 2.47e-13 72.4
Msa0705190 AT1G21980.1 29.688 128 67 1 59 186 142 246 7.30e-12 67.8
Msa0705190 AT3G09920.3 31.519 349 216 8 52 384 42 383 9.73e-38 145
Msa0705190 AT3G09920.4 31.519 349 216 8 52 384 42 383 9.73e-38 145
Msa0705190 AT3G09920.2 31.519 349 216 8 52 384 42 383 9.73e-38 145
Msa0705190 AT3G09920.1 31.519 349 216 8 52 384 42 383 9.73e-38 145
Msa0705190 AT3G07960.3 32.268 313 175 6 62 354 27 322 5.94e-37 142
Msa0705190 AT3G07960.3 42.222 90 52 0 61 150 118 207 3.85e-13 71.6
Msa0705190 AT3G07960.2 32.268 313 175 6 62 354 27 322 6.31e-37 142
Msa0705190 AT3G07960.2 42.222 90 52 0 61 150 118 207 3.72e-13 71.6
Msa0705190 AT3G07960.4 32.268 313 175 6 62 354 27 322 6.31e-37 142
Msa0705190 AT3G07960.4 42.222 90 52 0 61 150 118 207 3.72e-13 71.6
Msa0705190 AT1G77740.2 40.500 200 110 3 62 261 77 267 8.94e-37 142
Msa0705190 AT1G77740.2 32.749 171 110 2 154 324 77 242 2.10e-19 90.9
Msa0705190 AT1G77740.2 35.185 108 62 1 61 168 168 267 1.29e-12 70.1
Msa0705190 AT1G77740.1 40.500 200 110 3 62 261 77 267 8.94e-37 142
Msa0705190 AT1G77740.1 32.749 171 110 2 154 324 77 242 2.10e-19 90.9
Msa0705190 AT1G77740.1 35.185 108 62 1 61 168 168 267 1.29e-12 70.1
Msa0705190 AT3G07960.1 32.268 313 175 6 62 354 27 322 1.15e-36 142
Msa0705190 AT3G07960.1 42.222 90 52 0 61 150 118 207 4.41e-13 71.2
Msa0705190 AT2G41210.1 39.810 211 112 4 57 253 60 269 1.52e-36 142
Msa0705190 AT2G41210.1 30.964 197 112 1 106 302 68 240 3.13e-23 102
Msa0705190 AT2G41210.1 36.429 140 88 1 177 316 70 208 1.37e-16 82.4
Msa0705190 AT3G56960.2 39.500 200 111 3 62 252 72 270 1.13e-34 136
Msa0705190 AT3G56960.2 34.104 173 113 1 106 278 70 241 4.11e-28 117
Msa0705190 AT3G56960.2 32.515 163 109 1 154 316 72 233 9.79e-20 92.0
Msa0705190 AT3G56960.1 39.500 200 111 3 62 252 72 270 1.13e-34 136
Msa0705190 AT3G56960.1 34.104 173 113 1 106 278 70 241 4.11e-28 117
Msa0705190 AT3G56960.1 32.515 163 109 1 154 316 72 233 9.79e-20 92.0
Msa0705190 AT1G21920.2 36.612 183 115 1 102 284 168 349 6.67e-32 124
Msa0705190 AT1G21920.2 34.653 202 119 3 148 339 168 366 1.39e-27 112
Msa0705190 AT1G21920.2 33.663 202 110 1 60 261 172 349 4.79e-26 108
Msa0705190 AT1G21920.1 36.612 183 115 1 102 284 168 349 1.28e-31 124
Msa0705190 AT1G21920.1 34.673 199 117 3 148 336 168 363 3.39e-27 112
Msa0705190 AT1G21920.1 33.663 202 110 1 60 261 172 349 7.27e-26 108
Msa0705190 AT2G35170.1 30.483 269 173 5 40 298 151 415 1.68e-31 125
Msa0705190 AT2G35170.1 35.503 169 106 2 153 321 225 390 9.74e-23 100
Msa0705190 AT2G35170.1 31.579 114 78 0 189 302 214 327 3.34e-11 65.1
Msa0705190 AT4G17080.1 36.453 203 122 3 84 284 235 432 3.31e-31 124
Msa0705190 AT4G17080.1 38.150 173 106 1 61 233 256 427 3.14e-28 116
Msa0705190 AT4G17080.1 35.754 179 112 2 141 319 244 419 3.08e-25 107
Msa0705190 AT4G17080.1 30.952 126 87 0 62 187 303 428 3.77e-16 80.5
Msa0705190 AT4G17080.1 33.333 111 74 0 58 168 322 432 1.16e-11 66.6
Msa0705190 AT1G77660.1 36.364 176 111 1 102 277 172 346 1.43e-29 119
Msa0705190 AT1G77660.1 36.872 179 112 1 60 238 176 353 1.17e-27 113
Msa0705190 AT1G77660.1 38.710 155 94 1 148 302 172 325 2.71e-23 101
Msa0705190 AT2G26420.2 40.761 184 85 3 63 246 54 213 8.48e-29 119
Msa0705190 AT2G26420.2 35.404 161 103 1 106 266 51 210 5.13e-23 101
Msa0705190 AT2G26420.2 35.211 142 91 1 59 200 73 213 1.76e-20 94.0
Msa0705190 AT2G26420.2 31.677 161 109 1 155 315 54 213 1.13e-17 85.5
Msa0705190 AT2G26420.2 34.058 138 68 1 202 316 54 191 4.73e-16 80.5
Msa0705190 AT2G26420.3 40.761 184 85 3 63 246 54 213 8.48e-29 119
Msa0705190 AT2G26420.3 35.404 161 103 1 106 266 51 210 5.13e-23 101
Msa0705190 AT2G26420.3 35.211 142 91 1 59 200 73 213 1.76e-20 94.0
Msa0705190 AT2G26420.3 31.677 161 109 1 155 315 54 213 1.13e-17 85.5
Msa0705190 AT2G26420.3 34.058 138 68 1 202 316 54 191 4.73e-16 80.5
Msa0705190 AT2G26420.1 40.761 184 85 3 63 246 54 213 9.90e-29 119
Msa0705190 AT2G26420.1 35.404 161 103 1 106 266 51 210 5.70e-23 101
Msa0705190 AT2G26420.1 35.211 142 91 1 59 200 73 213 1.97e-20 94.0
Msa0705190 AT2G26420.1 31.677 161 109 1 155 315 54 213 1.11e-17 85.5
Msa0705190 AT2G26420.1 34.058 138 68 1 202 316 54 191 4.85e-16 80.5

Find 115 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATGATGGGAGAGGGATTT+TGG 0.132408 4_4:-77730571 Msa0705190:CDS
CGGTTGCCGGAGTTGATTTA+TGG 0.180495 4_4:+77732744 None:intergenic
GGGATGTTTACACTGGAAAT+TGG 0.243578 4_4:-77730608 Msa0705190:CDS
TAAAGAAGAGATCGTGTATA+TGG 0.254713 4_4:-77729999 Msa0705190:CDS
TACCTCCATCCTTCCAAGTT+TGG 0.255059 4_4:+77730763 None:intergenic
GATTCAGATAATAGCTTTGA+TGG 0.263410 4_4:+77729963 None:intergenic
ACCTCCATCCTTCCAAGTTT+GGG 0.269330 4_4:+77730764 None:intergenic
TTCTTCACTTCGTCGGTTGC+CGG 0.302924 4_4:+77732731 None:intergenic
TTCAAATAAAGCTGAGTCTA+AGG 0.307919 4_4:-77730160 Msa0705190:CDS
AATGGACTTATACATGGATT+TGG 0.320121 4_4:-77730372 Msa0705190:CDS
GGGATGTCTACATCGGAAAT+TGG 0.328664 4_4:-77730467 Msa0705190:CDS
GAGGTAGCTACACTGGAAAT+TGG 0.329352 4_4:-77730746 Msa0705190:CDS
CAGAGAAAGGACTGATGATA+TGG 0.336491 4_4:-77730221 Msa0705190:CDS
TACGAGGGTGATTGGGTTGA+TGG 0.337164 4_4:-77730876 Msa0705190:CDS
CCTTGTCGCCGGTGGATTTA+CGG 0.350069 4_4:+77732798 None:intergenic
GCTAATGGGGATGTCGATAT+TGG 0.363580 4_4:-77730336 Msa0705190:CDS
GAGTTTATAGATATGCTAAT+GGG 0.365013 4_4:-77730350 Msa0705190:CDS
AATGGACTTGTACATGGATA+TGG 0.366109 4_4:-77730651 Msa0705190:CDS
GGAGTTTATAGATATGCTAA+TGG 0.368674 4_4:-77730351 Msa0705190:CDS
AATGGACTTAGACATGGATT+TGG 0.376853 4_4:-77730510 Msa0705190:CDS
GAGTTTATAGATTTGCAAAT+GGG 0.384860 4_4:-77730488 Msa0705190:CDS
GATCAATATACGAGGGTGAT+TGG 0.385479 4_4:-77730884 Msa0705190:CDS
GAACAATATACGAGGGTGAT+TGG 0.386941 4_4:-77730260 Msa0705190:CDS
GGTTGCCGGAGTTGATTTAT+GGG 0.389181 4_4:+77732745 None:intergenic
AGTTGATTTATGGGTGGCTT+TGG 0.393637 4_4:+77732754 None:intergenic
GGCATCTGGTGTGAGCCCTT+TGG 0.401200 4_4:-77730139 Msa0705190:CDS
AGAAAATACACATGCTCAAA+TGG 0.401828 4_4:-77730282 Msa0705190:CDS
AAAGACAACATGGATGGAAC+AGG 0.409911 4_4:-77730441 Msa0705190:CDS
TTTGATGGTTGTTTGTCAAA+TGG 0.417735 4_4:-77730528 Msa0705190:CDS
TTCGATGGTTGTTGGTCAAA+TGG 0.420416 4_4:-77730390 Msa0705190:CDS
GGGACTGCGACTTGGGCTAA+TGG 0.422911 4_4:-77730699 Msa0705190:CDS
TTGGTGGCCAGCAGCTTATG+TGG 0.434386 4_4:+77732773 None:intergenic
TTTGATGGTTGCTGGTCAAA+TGG 0.435014 4_4:-77730669 Msa0705190:CDS
GGAGTTTATAGATTTGCAAA+TGG 0.437498 4_4:-77730489 Msa0705190:CDS
AGATGTTCTACTATTCAAAC+GGG 0.442897 4_4:-77730974 Msa0705190:CDS
TGATTTATGGGTGGCTTTGG+TGG 0.443228 4_4:+77732757 None:intergenic
GAGCCCTTTGGTTCTCGAAT+GGG 0.444739 4_4:-77730127 Msa0705190:CDS
GGATTTACGGTCAGTTTGCT+TGG 0.448178 4_4:+77732811 None:intergenic
CGGAAAAGGGACTGCGACTT+GGG 0.448804 4_4:-77730706 Msa0705190:CDS
AACAATATACGAGGGTGATT+GGG 0.451603 4_4:-77730259 Msa0705190:CDS
ATGGCAATGGCACCCAAACT+TGG 0.453922 4_4:-77730776 Msa0705190:CDS
AAAGCTGAGTCTAAGGCATC+TGG 0.456725 4_4:-77730153 Msa0705190:CDS
AGGTAGCTACACTGGAAATT+GGG 0.461160 4_4:-77730745 Msa0705190:CDS
ATGTCGATATTGGTAACTTC+AGG 0.462222 4_4:-77730326 Msa0705190:CDS
GAGATGTTCTACTATTCAAA+CGG 0.464322 4_4:-77730975 Msa0705190:CDS
GGAGTTTATAGATCTGCAAA+TGG 0.464834 4_4:-77730630 Msa0705190:CDS
ATCAATATACGAGGGTGATT+GGG 0.465727 4_4:-77730883 Msa0705190:CDS
GTGTGGCTTCTTCACTTCGT+CGG 0.472255 4_4:+77732724 None:intergenic
TGGAACAGGGATTATGAGTT+GGG 0.472804 4_4:-77730427 Msa0705190:CDS
ATTTGAAATCTGCAATGAAG+TGG 0.473202 4_4:+77730176 None:intergenic
TGGTCAAATGGACTTATACA+TGG 0.474958 4_4:-77730378 Msa0705190:CDS
GAGTTTATAGATCTGCAAAT+GGG 0.475929 4_4:-77730629 Msa0705190:CDS
AAAATAGACAGAATGGATGA+TGG 0.482430 4_4:-77730585 Msa0705190:CDS
GACAGAATGGATGATGGGAG+AGG 0.485525 4_4:-77730579 Msa0705190:CDS
TGTCTATGTTGGTAACTTCA+AGG 0.486468 4_4:-77730949 Msa0705190:CDS
TGAGCCCTTTGGTTCTCGAA+TGG 0.488472 4_4:-77730128 Msa0705190:CDS
ACGGAAAAGGGACTGCGACT+TGG 0.489434 4_4:-77730707 Msa0705190:CDS
TGCAATGAAGTGGAACCATC+AGG 0.492197 4_4:+77730186 None:intergenic
CAAGGCTCCACATAAGCTGC+TGG 0.492954 4_4:-77732780 Msa0705190:CDS
TTCTTTGAGAATTGCCGTCA+TGG 0.499107 4_4:-77730795 Msa0705190:CDS
GGACTGCTCATACAATCATC+AGG 0.504986 4_4:-77730837 Msa0705190:CDS
AAAGAAGAGATCGTGTATAT+GGG 0.505023 4_4:-77729998 Msa0705190:CDS
GCGATGTCTTCGATGGTTGT+TGG 0.515994 4_4:-77730398 Msa0705190:CDS
CATCGTTTCTTGTAAACTGT+AGG 0.517692 4_4:-77731004 Msa0705190:CDS
TGGGTTGATGGAGAGATGAC+AGG 0.520799 4_4:-77730864 Msa0705190:CDS
GGGATTATGAGTTGGGCTAC+CGG 0.525267 4_4:-77730420 Msa0705190:CDS
ACAGAATGGATGATGGGAGA+GGG 0.526125 4_4:-77730578 Msa0705190:CDS
ATGGAACAGGGATTATGAGT+TGG 0.528516 4_4:-77730428 Msa0705190:CDS
GAGAATTGCCGTCATGGCAA+TGG 0.531830 4_4:-77730789 Msa0705190:CDS
AGAGAAAGGACTGATGATAT+GGG 0.534627 4_4:-77730220 Msa0705190:CDS
CCGTAAATCCACCGGCGACA+AGG 0.536931 4_4:-77732798 Msa0705190:CDS
TTTGGGTGCCATTGCCATGA+CGG 0.541667 4_4:+77730781 None:intergenic
GGCACCCAAACTTGGAAGGA+TGG 0.545646 4_4:-77730768 Msa0705190:CDS
AAATAGACAGAATGGATGAT+GGG 0.549899 4_4:-77730584 Msa0705190:CDS
GGAAAGTACACATGGTCAGA+TGG 0.558174 4_4:-77730906 Msa0705190:CDS
CTTATGTGGAGCCTTGTCGC+CGG 0.558415 4_4:+77732787 None:intergenic
ATGTGGAGCCTTGTCGCCGG+TGG 0.562717 4_4:+77732790 None:intergenic
TTGTCAAATGGACTTAGACA+TGG 0.568143 4_4:-77730516 Msa0705190:CDS
AGTTTATAGATTTGCAAATG+GGG 0.568661 4_4:-77730487 Msa0705190:CDS
AAATTGGAAAATAGACAGAA+TGG 0.571082 4_4:-77730592 Msa0705190:CDS
TCAAACGGGGATGTCTATGT+TGG 0.574532 4_4:-77730960 Msa0705190:CDS
GCTACCGGCGATGTCTTCGA+TGG 0.576597 4_4:-77730405 Msa0705190:CDS
TTCAGGAGTAAACTTCTCCA+TGG 0.576629 4_4:-77730309 Msa0705190:CDS
AAGGATGGAGGTAGCTACAC+TGG 0.576669 4_4:-77730753 Msa0705190:CDS
GCAAATGGGGATGTTTACAC+TGG 0.581474 4_4:-77730615 Msa0705190:CDS
GATGGAGAGATGACAGGGAA+AGG 0.581963 4_4:-77730858 Msa0705190:CDS
TGCCGGAGTTGATTTATGGG+TGG 0.582432 4_4:+77732748 None:intergenic
TGGATAAAAGACAACATGGA+TGG 0.584838 4_4:-77730447 Msa0705190:CDS
CAATGGCACCCAAACTTGGA+AGG 0.585341 4_4:-77730772 Msa0705190:CDS
TTCAAGGATGAACTTCCTCA+TGG 0.590492 4_4:-77730933 Msa0705190:CDS
GATGAACTTCCTCATGGCAA+AGG 0.593188 4_4:-77730927 Msa0705190:CDS
TATTCCCATTCGAGAACCAA+AGG 0.596199 4_4:+77730123 None:intergenic
TGGTCAAATGGACTTGTACA+TGG 0.597343 4_4:-77730657 Msa0705190:CDS
AGCCACCCATAAATCAACTC+CGG 0.601961 4_4:-77732750 Msa0705190:CDS
ACAACCATCGAAGACATCGC+CGG 0.607359 4_4:+77730401 None:intergenic
ATTCCCATTCGAGAACCAAA+GGG 0.607945 4_4:+77730124 None:intergenic
GGGTTGATGGAGAGATGACA+GGG 0.608966 4_4:-77730863 Msa0705190:CDS
CTCGAATGGGAATATATGCA+AGG 0.614374 4_4:-77730114 Msa0705190:CDS
TGGGCCCTTCAAAATCCTGA+TGG 0.617949 4_4:-77730201 Msa0705190:CDS
AGTTTATAGATATGCTAATG+GGG 0.619790 4_4:-77730349 Msa0705190:CDS
CTCAAATGGAACAATATACG+AGG 0.634118 4_4:-77730268 Msa0705190:CDS
AGTTTATAGATCTGCAAATG+GGG 0.639593 4_4:-77730628 Msa0705190:CDS
GTGTACTTTCCTTTGCCATG+AGG 0.651733 4_4:+77730918 None:intergenic
CAAACTGACCGTAAATCCAC+CGG 0.652448 4_4:-77732806 Msa0705190:CDS
GCAAATGGGGATGTCTACAT+CGG 0.652522 4_4:-77730474 Msa0705190:CDS
AAATTGGATAAAAGACAACA+TGG 0.660956 4_4:-77730451 Msa0705190:CDS
AAGACAACATGGATGGAACA+GGG 0.663275 4_4:-77730440 Msa0705190:CDS
ACCCAAACTTGGAAGGATGG+AGG 0.668025 4_4:-77730765 Msa0705190:CDS
GTCAGATGGATCAATATACG+AGG 0.688571 4_4:-77730892 Msa0705190:CDS
ATGGCAAAGGAAAGTACACA+TGG 0.689912 4_4:-77730914 Msa0705190:CDS
TCAAATGGAACAATATACGA+GGG 0.706226 4_4:-77730267 Msa0705190:CDS
TCAGATGGATCAATATACGA+GGG 0.726714 4_4:-77730891 Msa0705190:CDS
ATCATCAGGAGAAATGTACG+AGG 0.734911 4_4:-77730823 Msa0705190:CDS
TCATCAGGAGAAATGTACGA+GGG 0.736398 4_4:-77730822 Msa0705190:CDS
GATGTTCTACTATTCAAACG+GGG 0.785195 4_4:-77730973 Msa0705190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATAAGTGAGTAAATAT+TGG - chr4_4:77730456-77730475 Msa0705190:CDS 15.0%
!! ATAAAAGAGAGTAATTTAAT+AGG + chr4_4:77730527-77730546 None:intergenic 15.0%
!! TAATATGCATAATATATGTA+AGG + chr4_4:77730956-77730975 None:intergenic 15.0%
!! ATAACAAAAATCTGTTTAAA+TGG + chr4_4:77731251-77731270 None:intergenic 15.0%
!! TATTTCATACTAGTAATTTA+GGG + chr4_4:77731338-77731357 None:intergenic 15.0%
!! ATATTTCATACTAGTAATTT+AGG + chr4_4:77731339-77731358 None:intergenic 15.0%
!! TAGTATGAAATATAAATAGA+GGG - chr4_4:77731346-77731365 Msa0705190:intron 15.0%
!!! TGTTTTGATATTTCTTTCAA+TGG + chr4_4:77730390-77730409 None:intergenic 20.0%
!! ACATTAGAGAGTTTATATTA+CGG - chr4_4:77730407-77730426 Msa0705190:CDS 20.0%
!! TTGAAACTCCATTTATATTT+CGG + chr4_4:77730701-77730720 None:intergenic 20.0%
!! CGAAATTAAAAAAAACCAAA+TGG - chr4_4:77730822-77730841 Msa0705190:CDS 20.0%
!!! GATAATCACTTTTTTTTTTG+TGG - chr4_4:77730911-77730930 Msa0705190:CDS 20.0%
!! CTAGTATGAAATATAAATAG+AGG - chr4_4:77731345-77731364 Msa0705190:intron 20.0%
!!! TAGTATTATTTTCGATTTTG+TGG - chr4_4:77731520-77731539 Msa0705190:intron 20.0%
!!! TATTGTAGTTTGTAAATGAA+TGG + chr4_4:77731706-77731725 None:intergenic 20.0%
!! TAATAACAAGAACAAAAAAC+AGG - chr4_4:77732744-77732763 Msa0705190:CDS 20.0%
! TGAAATCTCTCAAAATTCAA+AGG - chr4_4:77730184-77730203 Msa0705190:CDS 25.0%
!!! TGTTTTAAGTTGAGTATTAG+AGG - chr4_4:77730274-77730293 Msa0705190:CDS 25.0%
!!! GTTTTAAGTTGAGTATTAGA+GGG - chr4_4:77730275-77730294 Msa0705190:CDS 25.0%
! AGTATTAGAGGGAATTAAAA+GGG - chr4_4:77730286-77730305 Msa0705190:CDS 25.0%
! ATTTATCAAACTGATGAGAT+AGG + chr4_4:77730442-77730461 None:intergenic 25.0%
!! TTGGAACAAAAACAAGTTTT+AGG - chr4_4:77730475-77730494 Msa0705190:CDS 25.0%
! TTACAAATCATCATTTGACA+CGG + chr4_4:77730552-77730571 None:intergenic 25.0%
!!! GTTTGAAATGATTTGTTAGA+AGG - chr4_4:77730580-77730599 Msa0705190:CDS 25.0%
!!! AATGATTTGTTAGAAGGATA+TGG - chr4_4:77730586-77730605 Msa0705190:CDS 25.0%
! ATAGATACCCGAAATATAAA+TGG - chr4_4:77730690-77730709 Msa0705190:CDS 25.0%
! TGAAACTCCATTTATATTTC+GGG + chr4_4:77730700-77730719 None:intergenic 25.0%
! TCAAAAAAAAAATTGTGCAG+CGG - chr4_4:77730717-77730736 Msa0705190:CDS 25.0%
!!! TTTAATTTGACTTGACCATT+TGG + chr4_4:77730840-77730859 None:intergenic 25.0%
!!! GTTTCTTTTAAAAGCGATTT+AGG + chr4_4:77731312-77731331 None:intergenic 25.0%
! GATTAAAAGAATCGAAAAGT+CGG - chr4_4:77731415-77731434 Msa0705190:intron 25.0%
! TGGGAAAAAGATAAAAAAGA+CGG - chr4_4:77732061-77732080 Msa0705190:intron 25.0%
! AAAGATAAAAAAGACGGAAA+AGG - chr4_4:77732067-77732086 Msa0705190:intron 25.0%
! AAGATAAAAAAGACGGAAAA+GGG - chr4_4:77732068-77732087 Msa0705190:intron 25.0%
! AAATTGGAAAATAGACAGAA+TGG - chr4_4:77732195-77732214 Msa0705190:intron 25.0%
! GAGTTTATAGATTTGCAAAT+GGG - chr4_4:77732299-77732318 Msa0705190:intron 25.0%
! AGTTTATAGATTTGCAAATG+GGG - chr4_4:77732300-77732319 Msa0705190:intron 25.0%
! AAATTGGATAAAAGACAACA+TGG - chr4_4:77732336-77732355 Msa0705190:intron 25.0%
! GAGTTTATAGATATGCTAAT+GGG - chr4_4:77732437-77732456 Msa0705190:intron 25.0%
! AGTTTATAGATATGCTAATG+GGG - chr4_4:77732438-77732457 Msa0705190:intron 25.0%
!!! ACGTTGGAATGGAATTTTTT+GGG + chr4_4:77730156-77730175 None:intergenic 30.0%
GAGTATTAGAGGGAATTAAA+AGG - chr4_4:77730285-77730304 Msa0705190:CDS 30.0%
AGTCACATAAATTTGCAGAA+TGG - chr4_4:77730349-77730368 Msa0705190:CDS 30.0%
GTCACATAAATTTGCAGAAT+GGG - chr4_4:77730350-77730369 Msa0705190:CDS 30.0%
TACAAATCATCATTTGACAC+GGG + chr4_4:77730551-77730570 None:intergenic 30.0%
GGATATGGTGATATGATTTA+TGG - chr4_4:77730601-77730620 Msa0705190:CDS 30.0%
TGGTTACGATATATGTAGAA+TGG - chr4_4:77730663-77730682 Msa0705190:CDS 30.0%
TAACTTTGTTGCAAAAAGTG+AGG - chr4_4:77731103-77731122 Msa0705190:intron 30.0%
! TGAGATTTTGTGTGATTGTT+TGG - chr4_4:77731141-77731160 Msa0705190:intron 30.0%
!! TCTTTTAAAAGCGATTTAGG+TGG + chr4_4:77731309-77731328 None:intergenic 30.0%
TTCTAAGTGCTAACAAACAA+GGG + chr4_4:77731640-77731659 None:intergenic 30.0%
ATTCTAAGTGCTAACAAACA+AGG + chr4_4:77731641-77731660 None:intergenic 30.0%
GAGATGTTCTACTATTCAAA+CGG - chr4_4:77731812-77731831 Msa0705190:intron 30.0%
AGATGTTCTACTATTCAAAC+GGG - chr4_4:77731813-77731832 Msa0705190:intron 30.0%
GAGTTTATAGATCTGCAAAT+GGG - chr4_4:77732158-77732177 Msa0705190:intron 30.0%
AGTTTATAGATCTGCAAATG+GGG - chr4_4:77732159-77732178 Msa0705190:intron 30.0%
AAAATAGACAGAATGGATGA+TGG - chr4_4:77732202-77732221 Msa0705190:intron 30.0%
AAATAGACAGAATGGATGAT+GGG - chr4_4:77732203-77732222 Msa0705190:intron 30.0%
! TTTGATGGTTGTTTGTCAAA+TGG - chr4_4:77732259-77732278 Msa0705190:intron 30.0%
GGAGTTTATAGATTTGCAAA+TGG - chr4_4:77732298-77732317 Msa0705190:intron 30.0%
! AATGGACTTATACATGGATT+TGG - chr4_4:77732415-77732434 Msa0705190:intron 30.0%
GGAGTTTATAGATATGCTAA+TGG - chr4_4:77732436-77732455 Msa0705190:intron 30.0%
AGAAAATACACATGCTCAAA+TGG - chr4_4:77732505-77732524 Msa0705190:intron 30.0%
TCAAATGGAACAATATACGA+GGG - chr4_4:77732520-77732539 Msa0705190:intron 30.0%
GATAAAAAAGTGACAGAGAA+AGG - chr4_4:77732553-77732572 Msa0705190:intron 30.0%
! ATTTGAAATCTGCAATGAAG+TGG + chr4_4:77732614-77732633 None:intergenic 30.0%
TTCAAATAAAGCTGAGTCTA+AGG - chr4_4:77732627-77732646 Msa0705190:intron 30.0%
TAAAGAAGAGATCGTGTATA+TGG - chr4_4:77732788-77732807 Msa0705190:CDS 30.0%
AAAGAAGAGATCGTGTATAT+GGG - chr4_4:77732789-77732808 Msa0705190:CDS 30.0%
ATTCAAGGTTTGAATCTGTG+TGG + chr4_4:77730083-77730102 None:intergenic 35.0%
TCTCTCAAAGCAACAATTCA+AGG + chr4_4:77730098-77730117 None:intergenic 35.0%
!!! GACGTTGGAATGGAATTTTT+TGG + chr4_4:77730157-77730176 None:intergenic 35.0%
CTTGTTAGATCGATAGAGTA+TGG - chr4_4:77730643-77730662 Msa0705190:CDS 35.0%
CATAATATATGTAAGGTCCG+AGG + chr4_4:77730949-77730968 None:intergenic 35.0%
! GATTTTGTGTGATTGTTTGG+TGG - chr4_4:77731144-77731163 Msa0705190:intron 35.0%
TAAAAGAATCGAAAAGTCGG+AGG - chr4_4:77731418-77731437 Msa0705190:intron 35.0%
CATCTATCTGCATATGTGAT+GGG - chr4_4:77731595-77731614 Msa0705190:intron 35.0%
!! TGCATATGTGATGGGTTTAT+CGG - chr4_4:77731603-77731622 Msa0705190:intron 35.0%
CATCGTTTCTTGTAAACTGT+AGG - chr4_4:77731783-77731802 Msa0705190:intron 35.0%
GATGTTCTACTATTCAAACG+GGG - chr4_4:77731814-77731833 Msa0705190:intron 35.0%
!! TGTCTATGTTGGTAACTTCA+AGG - chr4_4:77731838-77731857 Msa0705190:intron 35.0%
TCAGATGGATCAATATACGA+GGG - chr4_4:77731896-77731915 Msa0705190:intron 35.0%
ATCAATATACGAGGGTGATT+GGG - chr4_4:77731904-77731923 Msa0705190:intron 35.0%
!! GCTAATGGTGATGTTTTTGA+TGG - chr4_4:77732103-77732122 Msa0705190:intron 35.0%
! AATGGACTTGTACATGGATA+TGG - chr4_4:77732136-77732155 Msa0705190:intron 35.0%
GGAGTTTATAGATCTGCAAA+TGG - chr4_4:77732157-77732176 Msa0705190:intron 35.0%
!! GCTAATGGTGATGTTTTTGA+TGG - chr4_4:77732103-77732122 Msa0705190:intron 35.0%
! TTGTCAAATGGACTTAGACA+TGG - chr4_4:77732271-77732290 Msa0705190:intron 35.0%
! AATGGACTTAGACATGGATT+TGG - chr4_4:77732277-77732296 Msa0705190:intron 35.0%
TGGATAAAAGACAACATGGA+TGG - chr4_4:77732340-77732359 Msa0705190:intron 35.0%
! TGGTCAAATGGACTTATACA+TGG - chr4_4:77732409-77732428 Msa0705190:intron 35.0%
! ATGTCGATATTGGTAACTTC+AGG - chr4_4:77732461-77732480 Msa0705190:intron 35.0%
! ATGTGTATTTTCTGTTGCCA+TGG + chr4_4:77732498-77732517 None:intergenic 35.0%
CTCAAATGGAACAATATACG+AGG - chr4_4:77732519-77732538 Msa0705190:intron 35.0%
AACAATATACGAGGGTGATT+GGG - chr4_4:77732528-77732547 Msa0705190:intron 35.0%
AGAGAAAGGACTGATGATAT+GGG - chr4_4:77732567-77732586 Msa0705190:intron 35.0%
! AGTTGATTTATGGGTGGCTT+TGG + chr4_4:77730036-77730055 None:intergenic 40.0%
AGATTTCACGAACAAGACGT+TGG + chr4_4:77730172-77730191 None:intergenic 40.0%
ATGACTAGAAATAGAGCGAG+TGG - chr4_4:77730316-77730335 Msa0705190:CDS 40.0%
TGACTAGAAATAGAGCGAGT+GGG - chr4_4:77730317-77730336 Msa0705190:CDS 40.0%
TTTGCAGAATGGGTCATTGA+AGG - chr4_4:77730360-77730379 Msa0705190:CDS 40.0%
TTGCAGAATGGGTCATTGAA+GGG - chr4_4:77730361-77730380 Msa0705190:CDS 40.0%
AAAATTGTGCAGCGGAATTG+TGG - chr4_4:77730725-77730744 Msa0705190:CDS 40.0%
AACACAAGAGCAGATTGCTT+AGG + chr4_4:77731477-77731496 None:intergenic 40.0%
AAATTCATGCTCTTGTGTGC+TGG - chr4_4:77731566-77731585 Msa0705190:intron 40.0%
GCATCTATCTGCATATGTGA+TGG - chr4_4:77731594-77731613 Msa0705190:intron 40.0%
TTCAAGGATGAACTTCCTCA+TGG - chr4_4:77731854-77731873 Msa0705190:intron 40.0%
! ATGGCAAAGGAAAGTACACA+TGG - chr4_4:77731873-77731892 Msa0705190:intron 40.0%
GTCAGATGGATCAATATACG+AGG - chr4_4:77731895-77731914 Msa0705190:intron 40.0%
GATCAATATACGAGGGTGAT+TGG - chr4_4:77731903-77731922 Msa0705190:intron 40.0%
ATCATCAGGAGAAATGTACG+AGG - chr4_4:77731964-77731983 Msa0705190:intron 40.0%
TCATCAGGAGAAATGTACGA+GGG - chr4_4:77731965-77731984 Msa0705190:intron 40.0%
! TTCTTTGAGAATTGCCGTCA+TGG - chr4_4:77731992-77732011 Msa0705190:intron 40.0%
AGGTAGCTACACTGGAAATT+GGG - chr4_4:77732042-77732061 Msa0705190:intron 40.0%
!! GTGATGTTTTTGATGGTTGC+TGG - chr4_4:77732110-77732129 Msa0705190:intron 40.0%
! TTTGATGGTTGCTGGTCAAA+TGG - chr4_4:77732118-77732137 Msa0705190:intron 40.0%
! TGGTCAAATGGACTTGTACA+TGG - chr4_4:77732130-77732149 Msa0705190:intron 40.0%
GGGATGTTTACACTGGAAAT+TGG - chr4_4:77732179-77732198 Msa0705190:intron 40.0%
AAAGACAACATGGATGGAAC+AGG - chr4_4:77732346-77732365 Msa0705190:intron 40.0%
AAGACAACATGGATGGAACA+GGG - chr4_4:77732347-77732366 Msa0705190:intron 40.0%
ATGGAACAGGGATTATGAGT+TGG - chr4_4:77732359-77732378 Msa0705190:intron 40.0%
TGGAACAGGGATTATGAGTT+GGG - chr4_4:77732360-77732379 Msa0705190:intron 40.0%
TTCGATGGTTGTTGGTCAAA+TGG - chr4_4:77732397-77732416 Msa0705190:intron 40.0%
TTCAGGAGTAAACTTCTCCA+TGG - chr4_4:77732478-77732497 Msa0705190:intron 40.0%
GAACAATATACGAGGGTGAT+TGG - chr4_4:77732527-77732546 Msa0705190:intron 40.0%
CAGAGAAAGGACTGATGATA+TGG - chr4_4:77732566-77732585 Msa0705190:intron 40.0%
! GGAACCATCAGGATTTTGAA+GGG + chr4_4:77732593-77732612 None:intergenic 40.0%
! TGGAACCATCAGGATTTTGA+AGG + chr4_4:77732594-77732613 None:intergenic 40.0%
ATTCCCATTCGAGAACCAAA+GGG + chr4_4:77732666-77732685 None:intergenic 40.0%
TATTCCCATTCGAGAACCAA+AGG + chr4_4:77732667-77732686 None:intergenic 40.0%
CTCGAATGGGAATATATGCA+AGG - chr4_4:77732673-77732692 Msa0705190:intron 40.0%
! GGATTTACGGTCAGTTTGCT+TGG + chr4_4:77729979-77729998 None:intergenic 45.0%
CAAACTGACCGTAAATCCAC+CGG - chr4_4:77729981-77730000 Msa0705190:CDS 45.0%
!! TGATTTATGGGTGGCTTTGG+TGG + chr4_4:77730033-77730052 None:intergenic 45.0%
AGCCACCCATAAATCAACTC+CGG - chr4_4:77730037-77730056 Msa0705190:CDS 45.0%
! GGTTGCCGGAGTTGATTTAT+GGG + chr4_4:77730045-77730064 None:intergenic 45.0%
TCACGAACAAGACGTTGGAA+TGG + chr4_4:77730167-77730186 None:intergenic 45.0%
AGAAATAGAGCGAGTGGGAA+AGG - chr4_4:77730322-77730341 Msa0705190:CDS 45.0%
TCATGTGAGCTTAGCTCAGT+TGG + chr4_4:77730985-77731004 None:intergenic 45.0%
AAGAATCGAAAAGTCGGAGG+AGG - chr4_4:77731421-77731440 Msa0705190:intron 45.0%
AAAAGTCGGAGGAGGAGAAA+AGG - chr4_4:77731429-77731448 Msa0705190:intron 45.0%
TCAAACGGGGATGTCTATGT+TGG - chr4_4:77731827-77731846 Msa0705190:intron 45.0%
GATGAACTTCCTCATGGCAA+AGG - chr4_4:77731860-77731879 Msa0705190:intron 45.0%
GTGTACTTTCCTTTGCCATG+AGG + chr4_4:77731872-77731891 None:intergenic 45.0%
! GGAAAGTACACATGGTCAGA+TGG - chr4_4:77731881-77731900 Msa0705190:intron 45.0%
GGACTGCTCATACAATCATC+AGG - chr4_4:77731950-77731969 Msa0705190:intron 45.0%
ACCTCCATCCTTCCAAGTTT+GGG + chr4_4:77732026-77732045 None:intergenic 45.0%
TACCTCCATCCTTCCAAGTT+TGG + chr4_4:77732027-77732046 None:intergenic 45.0%
GAGGTAGCTACACTGGAAAT+TGG - chr4_4:77732041-77732060 Msa0705190:intron 45.0%
GCAAATGGGGATGTTTACAC+TGG - chr4_4:77732172-77732191 Msa0705190:intron 45.0%
!! ACAGAATGGATGATGGGAGA+GGG - chr4_4:77732209-77732228 Msa0705190:intron 45.0%
! ATGGGAGAGGGATTTTGGAT+TGG - chr4_4:77732221-77732240 Msa0705190:intron 45.0%
! TGGGAGAGGGATTTTGGATT+GGG - chr4_4:77732222-77732241 Msa0705190:intron 45.0%
!! GGGATTTTGGATTGGGCTAA+TGG - chr4_4:77732229-77732248 Msa0705190:intron 45.0%
GCAAATGGGGATGTCTACAT+CGG - chr4_4:77732313-77732332 Msa0705190:intron 45.0%
GGGATGTCTACATCGGAAAT+TGG - chr4_4:77732320-77732339 Msa0705190:intron 45.0%
GCTAATGGGGATGTCGATAT+TGG - chr4_4:77732451-77732470 Msa0705190:intron 45.0%
TGCAATGAAGTGGAACCATC+AGG + chr4_4:77732604-77732623 None:intergenic 45.0%
! AAAGCTGAGTCTAAGGCATC+TGG - chr4_4:77732634-77732653 Msa0705190:intron 45.0%
!! ATAATAATAATAATAATAGA+TGG + chr4_4:77731755-77731774 None:intergenic 5.0%
! TGCCGGAGTTGATTTATGGG+TGG + chr4_4:77730042-77730061 None:intergenic 50.0%
! CGGTTGCCGGAGTTGATTTA+TGG + chr4_4:77730046-77730065 None:intergenic 50.0%
! TTCTTCACTTCGTCGGTTGC+CGG + chr4_4:77730059-77730078 None:intergenic 50.0%
GTGTGGCTTCTTCACTTCGT+CGG + chr4_4:77730066-77730085 None:intergenic 50.0%
TGTGGCCTGAGTTTGAACCT+CGG - chr4_4:77730929-77730948 Msa0705190:CDS 50.0%
AAGGTCCGAGGTTCAAACTC+AGG + chr4_4:77730937-77730956 None:intergenic 50.0%
GGAGGAGGAGAAAAGGAGAT+CGG - chr4_4:77731436-77731455 Msa0705190:intron 50.0%
GAGGAGGAGAAAAGGAGATC+GGG - chr4_4:77731437-77731456 Msa0705190:intron 50.0%
! ATGCTCTTGTGTGCTGGTAG+AGG - chr4_4:77731572-77731591 Msa0705190:intron 50.0%
!! TACGAGGGTGATTGGGTTGA+TGG - chr4_4:77731911-77731930 Msa0705190:intron 50.0%
!! TGGGTTGATGGAGAGATGAC+AGG - chr4_4:77731923-77731942 Msa0705190:intron 50.0%
!! GGGTTGATGGAGAGATGACA+GGG - chr4_4:77731924-77731943 Msa0705190:intron 50.0%
GATGGAGAGATGACAGGGAA+AGG - chr4_4:77731929-77731948 Msa0705190:intron 50.0%
GAGAATTGCCGTCATGGCAA+TGG - chr4_4:77731998-77732017 Msa0705190:intron 50.0%
!! TTTGGGTGCCATTGCCATGA+CGG + chr4_4:77732009-77732028 None:intergenic 50.0%
ATGGCAATGGCACCCAAACT+TGG - chr4_4:77732011-77732030 Msa0705190:intron 50.0%
CAATGGCACCCAAACTTGGA+AGG - chr4_4:77732015-77732034 Msa0705190:intron 50.0%
ACCCAAACTTGGAAGGATGG+AGG - chr4_4:77732022-77732041 Msa0705190:intron 50.0%
AAGGATGGAGGTAGCTACAC+TGG - chr4_4:77732034-77732053 Msa0705190:intron 50.0%
!! GACAGAATGGATGATGGGAG+AGG - chr4_4:77732208-77732227 Msa0705190:intron 50.0%
!!! GGATGATGGGAGAGGGATTT+TGG - chr4_4:77732216-77732235 Msa0705190:intron 50.0%
! GGGATTATGAGTTGGGCTAC+CGG - chr4_4:77732367-77732386 Msa0705190:intron 50.0%
ACAACCATCGAAGACATCGC+CGG + chr4_4:77732389-77732408 None:intergenic 50.0%
GCGATGTCTTCGATGGTTGT+TGG - chr4_4:77732389-77732408 Msa0705190:intron 50.0%
TGGGCCCTTCAAAATCCTGA+TGG - chr4_4:77732586-77732605 Msa0705190:intron 50.0%
!! TGAGCCCTTTGGTTCTCGAA+TGG - chr4_4:77732659-77732678 Msa0705190:intron 50.0%
!! GAGCCCTTTGGTTCTCGAAT+GGG - chr4_4:77732660-77732679 Msa0705190:intron 50.0%
! CCTTGTCGCCGGTGGATTTA+CGG + chr4_4:77729992-77730011 None:intergenic 55.0%
! CTTATGTGGAGCCTTGTCGC+CGG + chr4_4:77730003-77730022 None:intergenic 55.0%
CAAGGCTCCACATAAGCTGC+TGG - chr4_4:77730007-77730026 Msa0705190:CDS 55.0%
! TTGGTGGCCAGCAGCTTATG+TGG + chr4_4:77730017-77730036 None:intergenic 55.0%
GGCACCCAAACTTGGAAGGA+TGG - chr4_4:77732019-77732038 Msa0705190:intron 55.0%
ACGGAAAAGGGACTGCGACT+TGG - chr4_4:77732080-77732099 Msa0705190:intron 55.0%
CGGAAAAGGGACTGCGACTT+GGG - chr4_4:77732081-77732100 Msa0705190:intron 55.0%
CCGTAAATCCACCGGCGACA+AGG - chr4_4:77729989-77730008 Msa0705190:CDS 60.0%
! GGGACTGCGACTTGGGCTAA+TGG - chr4_4:77732088-77732107 Msa0705190:intron 60.0%
GCTACCGGCGATGTCTTCGA+TGG - chr4_4:77732382-77732401 Msa0705190:intron 60.0%
GGCATCTGGTGTGAGCCCTT+TGG - chr4_4:77732648-77732667 Msa0705190:intron 60.0%
! ATGTGGAGCCTTGTCGCCGG+TGG + chr4_4:77730000-77730019 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 77729966 77732843 77729966 ID=Msa0705190;Name=Msa0705190
chr4_4 mRNA 77729966 77732843 77729966 ID=Msa0705190-mRNA-1;Parent=Msa0705190;Name=Msa0705190-mRNA-1;_AED=0.22;_eAED=0.22;_QI=0|0|0|1|0|0|2|0|399
chr4_4 exon 77732739 77732843 77732739 ID=Msa0705190-mRNA-1:exon:24757;Parent=Msa0705190-mRNA-1
chr4_4 exon 77729966 77731060 77729966 ID=Msa0705190-mRNA-1:exon:24756;Parent=Msa0705190-mRNA-1
chr4_4 CDS 77732739 77732843 77732739 ID=Msa0705190-mRNA-1:cds;Parent=Msa0705190-mRNA-1
chr4_4 CDS 77729966 77731060 77729966 ID=Msa0705190-mRNA-1:cds;Parent=Msa0705190-mRNA-1
Gene Sequence

>Msa0705190

ATGGCTCGTACCAAGCAAACTGACCGTAAATCCACCGGCGACAAGGCTCCACATAAGCTGCTGGCCACCAAAGCCACCCATAAATCAACTCCGGCAACCGACGAATATCCATCTATTATTATTATTATTATTATAATCTCATCGTTTCTTGTAAACTGTAGGTTTAAAGAGATGTTCTACTATTCAAACGGGGATGTCTATGTTGGTAACTTCAAGGATGAACTTCCTCATGGCAAAGGAAAGTACACATGGTCAGATGGATCAATATACGAGGGTGATTGGGTTGATGGAGAGATGACAGGGAAAGGACTGCTCATACAATCATCAGGAGAAATGTACGAGGGTGAATTCTTTGAGAATTGCCGTCATGGCAATGGCACCCAAACTTGGAAGGATGGAGGTAGCTACACTGGAAATTGGGAAAAAGATAAAAAAGACGGAAAAGGGACTGCGACTTGGGCTAATGGTGATGTTTTTGATGGTTGCTGGTCAAATGGACTTGTACATGGATATGGAGTTTATAGATCTGCAAATGGGGATGTTTACACTGGAAATTGGAAAATAGACAGAATGGATGATGGGAGAGGGATTTTGGATTGGGCTAATGGTGATGTTTTTGATGGTTGTTTGTCAAATGGACTTAGACATGGATTTGGAGTTTATAGATTTGCAAATGGGGATGTCTACATCGGAAATTGGATAAAAGACAACATGGATGGAACAGGGATTATGAGTTGGGCTACCGGCGATGTCTTCGATGGTTGTTGGTCAAATGGACTTATACATGGATTTGGAGTTTATAGATATGCTAATGGGGATGTCGATATTGGTAACTTCAGGAGTAAACTTCTCCATGGCAACAGAAAATACACATGCTCAAATGGAACAATATACGAGGGTGATTGGGTTGATAAAAAAGTGACAGAGAAAGGACTGATGATATGGGCCCTTCAAAATCCTGATGGTTCCACTTCATTGCAGATTTCAAATAAAGCTGAGTCTAAGGCATCTGGTGTGAGCCCTTTGGTTCTCGAATGGGAATATATGCAAGGAGTTCTAATTGTTGAGAAAATTATACAGTATTCAGAAGTAACACATAATAATAACAAGAACAAAAAACAGGATGCATTTAGTGTGAAGAAAGTAAAGAAGAGATCGTGTATATGGGCATTTTTTAAGACCATCAAAGCTATTATCTGA
Protein sequence

>Msa0705190

MARTKQTDRKSTGDKAPHKLLATKATHKSTPATDEYPSIIIIIIIISSFLVNCRFKEMFYYSNGDVYVGNFKDELPHGKGKYTWSDGSIYEGDWVDGEMTGKGLLIQSSGEMYEGEFFENCRHGNGTQTWKDGGSYTGNWEKDKKDGKGTATWANGDVFDGCWSNGLVHGYGVYRSANGDVYTGNWKIDRMDDGRGILDWANGDVFDGCLSNGLRHGFGVYRFANGDVYIGNWIKDNMDGTGIMSWATGDVFDGCWSNGLIHGFGVYRYANGDVDIGNFRSKLLHGNRKYTCSNGTIYEGDWVDKKVTEKGLMIWALQNPDGSTSLQISNKAESKASGVSPLVLEWEYMQGVLIVEKIIQYSEVTHNNNKNKKQDAFSVKKVKKRSCIWAFFKTIKAII*