AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0659210

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0659210 MtrunA17_Chr8g0385241 62.937 429 74 5 53 397 4 431 3.01e-169 480
Msa0659210 MtrunA17_Chr8g0382971 68.156 358 104 4 40 397 6 353 3.62e-166 469
Msa0659210 MtrunA17_Chr8g0383551 55.844 385 118 7 42 397 6 367 2.09e-125 366
Msa0659210 MtrunA17_Chr4g0059911 62.102 314 107 6 53 362 3 308 5.52e-124 363
Msa0659210 MtrunA17_Chr8g0377671 59.055 254 103 1 59 312 19 271 1.19e-96 290
Msa0659210 MtrunA17_Chr8g0377671 50.000 232 92 2 106 314 20 250 4.29e-65 209
Msa0659210 MtrunA17_Chr8g0377671 47.273 165 86 1 54 218 106 269 6.15e-40 143
Msa0659210 MtrunA17_Chr8g0383331 77.439 164 35 2 150 312 2 164 3.52e-82 248
Msa0659210 MtrunA17_Chr8g0383331 43.506 154 85 2 59 211 3 155 8.18e-35 126
Msa0659210 MtrunA17_Chr8g0377191 60.784 204 78 2 51 254 4 205 1.92e-76 235
Msa0659210 MtrunA17_Chr8g0377191 52.577 194 91 1 107 300 13 205 9.53e-60 192
Msa0659210 MtrunA17_Chr8g0377191 40.650 123 71 1 200 322 13 133 1.69e-19 86.3
Msa0659210 MtrunA17_Chr8g0377191 62.500 48 18 0 267 314 11 58 2.67e-11 62.8
Msa0659210 MtrunA17_Chr8g0380791 43.429 350 166 10 50 386 4 334 1.54e-75 249
Msa0659210 MtrunA17_Chr2g0316611 47.368 285 144 3 38 322 15 293 2.11e-74 236
Msa0659210 MtrunA17_Chr2g0316611 37.407 270 122 3 60 283 105 373 7.36e-47 164
Msa0659210 MtrunA17_Chr2g0316631 46.667 285 146 3 38 322 15 293 9.34e-73 229
Msa0659210 MtrunA17_Chr2g0316631 49.600 125 63 0 60 184 174 298 9.35e-33 125
Msa0659210 MtrunA17_Chr2g0316631 59.740 77 30 1 46 121 228 304 2.01e-20 90.9
Msa0659210 MtrunA17_Chr6g0467061 45.985 274 146 2 41 314 1 272 6.37e-69 221
Msa0659210 MtrunA17_Chr6g0467061 40.299 201 96 1 57 257 154 330 2.09e-35 132
Msa0659210 MtrunA17_Chr6g0467061 34.653 202 107 3 151 328 18 218 1.26e-27 111
Msa0659210 MtrunA17_Chr6g0467061 80.488 41 8 0 60 100 295 335 1.31e-13 71.2
Msa0659210 MtrunA17_Chr8g0377211 50.000 166 57 4 53 218 14 153 1.84e-44 152
Msa0659210 MtrunA17_Chr8g0377211 52.857 140 65 1 173 312 17 155 1.58e-39 139
Msa0659210 MtrunA17_Chr8g0377211 44.203 138 52 3 150 264 18 153 1.62e-26 104
Msa0659210 MtrunA17_Chr8g0377211 55.102 49 22 0 56 104 107 155 1.58e-11 62.8
Msa0659210 MtrunA17_Chr8g0377221 39.732 224 88 4 56 237 1 219 1.95e-42 150
Msa0659210 MtrunA17_Chr8g0377221 48.299 147 61 2 60 191 74 220 3.07e-34 128
Msa0659210 MtrunA17_Chr8g0377221 42.017 119 69 0 195 313 1 119 5.85e-23 97.4
Msa0659210 MtrunA17_Chr8g0377221 27.234 235 155 3 102 321 1 234 7.04e-20 89.0
Msa0659210 MtrunA17_Chr8g0353121 41.799 189 109 1 60 248 53 240 6.34e-41 154
Msa0659210 MtrunA17_Chr8g0353121 37.791 172 106 1 104 275 51 221 5.42e-32 128
Msa0659210 MtrunA17_Chr8g0353121 34.595 185 116 2 153 337 54 233 6.29e-22 98.2
Msa0659210 MtrunA17_Chr3g0082501 42.541 181 103 1 60 240 64 243 8.06e-41 154
Msa0659210 MtrunA17_Chr3g0082501 32.370 173 93 2 126 275 61 232 2.53e-22 99.4
Msa0659210 MtrunA17_Chr3g0082501 32.979 188 121 2 150 337 62 244 3.56e-22 99.0
Msa0659210 MtrunA17_Chr1g0180261 31.742 356 219 8 45 379 37 389 1.63e-40 153
Msa0659210 MtrunA17_Chr1g0171491 39.894 188 112 1 60 247 61 247 1.68e-35 138
Msa0659210 MtrunA17_Chr1g0171491 32.995 197 108 1 104 300 59 231 4.66e-25 107
Msa0659210 MtrunA17_Chr1g0171491 32.836 201 134 1 70 270 48 247 2.03e-22 99.8
Msa0659210 MtrunA17_Chr1g0171491 32.086 187 120 3 132 313 37 221 1.25e-19 91.3
Msa0659210 MtrunA17_Chr1g0171491 44.444 90 50 0 59 148 152 241 5.19e-15 77.0
Msa0659210 MtrunA17_Chr1g0171491 31.646 158 72 2 60 204 130 264 7.37e-15 76.6
Msa0659210 MtrunA17_Chr3g0115111 42.529 174 98 2 58 230 55 227 2.97e-34 134
Msa0659210 MtrunA17_Chr3g0115111 30.392 204 134 4 133 335 40 236 3.48e-18 86.7
Msa0659210 MtrunA17_Chr5g0424111 37.563 197 115 2 90 279 174 369 2.91e-33 129
Msa0659210 MtrunA17_Chr5g0424111 38.418 177 108 1 55 231 192 367 5.79e-28 114
Msa0659210 MtrunA17_Chr5g0424111 36.723 177 109 2 143 319 188 361 7.84e-28 114
Msa0659210 MtrunA17_Chr5g0424111 34.043 141 91 1 189 329 187 325 1.29e-16 81.3
Msa0659210 MtrunA17_Chr5g0398011 53.097 113 53 0 58 170 33 145 7.32e-33 120
Msa0659210 MtrunA17_Chr5g0398011 41.176 119 70 0 196 314 32 150 2.37e-20 87.0
Msa0659210 MtrunA17_Chr5g0398011 73.333 45 12 0 60 104 104 148 1.17e-15 73.9
Msa0659210 MtrunA17_Chr7g0233351 36.559 186 115 2 99 282 183 367 5.48e-32 125
Msa0659210 MtrunA17_Chr7g0233351 35.323 201 106 1 59 259 191 367 1.07e-29 119
Msa0659210 MtrunA17_Chr7g0233351 37.017 181 109 3 139 317 177 354 1.65e-27 113
Msa0659210 MtrunA17_Chr7g0233351 34.234 111 73 0 56 166 257 367 9.08e-13 69.7
Msa0659210 MtrunA17_Chr8g0336161 35.912 181 115 1 102 282 193 372 6.12e-32 125
Msa0659210 MtrunA17_Chr8g0336161 36.788 193 118 2 45 237 185 373 3.81e-27 112
Msa0659210 MtrunA17_Chr8g0336161 38.095 168 101 2 150 317 195 359 4.61e-26 109
Msa0659210 MtrunA17_Chr3g0131001 43.258 178 100 1 58 235 53 229 7.41e-32 127
Msa0659210 MtrunA17_Chr3g0131001 32.558 172 111 2 152 323 55 221 2.98e-14 74.7
Msa0659210 MtrunA17_Chr5g0424301 41.212 165 96 1 60 224 69 232 1.64e-31 126
Msa0659210 MtrunA17_Chr5g0424301 34.132 167 109 1 104 270 67 232 2.73e-26 111
Msa0659210 MtrunA17_Chr5g0424301 31.250 160 109 1 152 311 69 227 3.11e-17 84.0
Msa0659210 MtrunA17_Chr4g0055371 33.493 209 136 2 79 287 147 352 1.54e-30 121
Msa0659210 MtrunA17_Chr4g0055371 33.831 201 109 2 59 259 171 347 3.17e-27 112
Msa0659210 MtrunA17_Chr4g0055371 35.393 178 112 2 140 317 160 334 3.20e-26 109
Msa0659210 MtrunA17_Chr4g0055371 31.111 135 93 0 59 193 217 351 1.78e-15 77.8
Msa0659210 MtrunA17_Chr4g0055371 33.333 135 88 1 185 319 158 290 2.73e-15 77.0
Msa0659210 MtrunA17_Chr4g0055371 34.234 111 73 0 56 166 237 347 2.18e-12 68.2
Msa0659210 MtrunA17_Chr7g0256301 40.116 172 102 1 60 231 71 241 1.97e-30 123
Msa0659210 MtrunA17_Chr7g0256301 35.632 174 111 1 104 277 69 241 9.15e-28 115
Msa0659210 MtrunA17_Chr7g0256301 30.247 162 112 1 152 313 71 231 8.85e-17 82.4
Msa0659210 MtrunA17_Chr8g0385201 41.304 184 102 4 60 239 30 211 3.45e-30 122
Msa0659210 MtrunA17_Chr8g0385201 34.659 176 113 2 106 280 30 204 9.34e-26 109
Msa0659210 MtrunA17_Chr8g0385201 38.000 150 91 2 57 206 51 198 4.45e-23 101
Msa0659210 MtrunA17_Chr8g0385201 31.844 179 120 2 148 325 26 203 7.80e-19 88.6
Msa0659210 MtrunA17_Chr3g0114791 34.659 176 114 1 100 275 179 353 4.61e-30 120
Msa0659210 MtrunA17_Chr3g0114791 36.313 179 113 1 58 236 183 360 2.93e-28 115
Msa0659210 MtrunA17_Chr3g0114791 33.889 180 116 2 140 319 173 349 2.21e-25 107
Msa0659210 MtrunA17_Chr3g0114791 33.582 134 87 1 186 319 172 303 2.08e-14 74.3
Msa0659210 MtrunA17_Chr5g0398021 52.885 104 49 0 60 163 13 116 1.43e-29 112
Msa0659210 MtrunA17_Chr5g0398021 36.975 119 75 0 196 314 10 128 8.06e-20 85.5
Msa0659210 MtrunA17_Chr5g0398021 35.185 108 69 1 103 210 10 116 6.94e-15 71.6
Msa0659210 MtrunA17_Chr8g0377201 66.216 74 25 0 242 315 1 74 7.85e-26 99.8
Msa0659210 MtrunA17_Chr8g0377201 58.065 62 26 0 196 257 1 62 1.46e-17 77.0
Msa0659210 MtrunA17_Chr8g0377201 56.923 65 27 1 151 215 3 66 2.78e-15 70.9
Msa0659210 MtrunA17_Chr8g0377201 60.784 51 20 0 56 106 23 73 6.68e-15 69.7
Msa0659210 MtrunA17_Chr3g0085921 73.333 60 16 0 191 250 2 61 3.39e-23 93.6
Msa0659210 MtrunA17_Chr3g0085921 65.574 61 20 1 144 204 2 61 3.04e-19 82.8
Msa0659210 MtrunA17_Chr3g0085921 34.862 109 48 1 236 344 1 86 9.12e-12 61.6
Msa0659210 MtrunA17_Chr1g0210831 34.146 123 81 0 62 184 615 737 3.14e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0659210 AT1G60890.1 36.416 346 184 7 59 386 10 337 1.22e-58 204
Msa0659210 AT1G60890.2 36.416 346 184 7 59 386 22 349 1.65e-58 204
Msa0659210 AT1G10900.2 36.937 333 187 6 59 386 10 324 2.54e-52 186
Msa0659210 AT1G10900.2 31.410 156 106 1 147 302 6 160 4.35e-16 80.9
Msa0659210 AT1G10900.2 29.730 148 81 2 189 313 1 148 3.08e-11 65.9
Msa0659210 AT1G10900.1 36.937 333 187 6 59 386 10 324 2.54e-52 186
Msa0659210 AT1G10900.1 31.410 156 106 1 147 302 6 160 4.35e-16 80.9
Msa0659210 AT1G10900.1 29.730 148 81 2 189 313 1 148 3.08e-11 65.9
Msa0659210 AT1G10900.3 36.937 333 187 6 59 386 10 324 2.54e-52 186
Msa0659210 AT1G10900.3 31.410 156 106 1 147 302 6 160 4.35e-16 80.9
Msa0659210 AT1G10900.3 29.730 148 81 2 189 313 1 148 3.08e-11 65.9
Msa0659210 AT3G09920.3 31.884 345 211 8 52 379 46 383 1.68e-38 147
Msa0659210 AT3G09920.4 31.884 345 211 8 52 379 46 383 1.68e-38 147
Msa0659210 AT3G09920.2 31.884 345 211 8 52 379 46 383 1.68e-38 147
Msa0659210 AT3G09920.1 31.884 345 211 8 52 379 46 383 1.68e-38 147
Msa0659210 AT1G21980.1 40.594 202 110 2 60 261 76 267 3.23e-38 146
Msa0659210 AT1G21980.1 30.000 240 142 5 152 387 76 293 1.51e-22 100
Msa0659210 AT1G21980.1 43.750 80 45 0 59 138 167 246 1.69e-13 72.8
Msa0659210 AT1G21980.1 29.688 128 67 1 57 184 142 246 4.38e-12 68.2
Msa0659210 AT3G07960.2 32.268 313 175 6 60 352 27 322 2.52e-37 143
Msa0659210 AT3G07960.2 42.222 90 52 0 59 148 118 207 2.98e-13 71.6
Msa0659210 AT3G07960.4 32.268 313 175 6 60 352 27 322 2.52e-37 143
Msa0659210 AT3G07960.4 42.222 90 52 0 59 148 118 207 2.98e-13 71.6
Msa0659210 AT3G07960.3 32.268 313 175 6 60 352 27 322 2.53e-37 143
Msa0659210 AT3G07960.3 42.222 90 52 0 59 148 118 207 3.14e-13 71.6
Msa0659210 AT3G07960.1 32.268 313 175 6 60 352 27 322 4.98e-37 143
Msa0659210 AT3G07960.1 42.222 90 52 0 59 148 118 207 3.32e-13 71.6
Msa0659210 AT1G77740.2 40.500 200 110 3 60 259 77 267 5.52e-37 143
Msa0659210 AT1G77740.2 28.390 236 152 4 152 387 77 295 3.05e-20 93.6
Msa0659210 AT1G77740.2 35.185 108 62 1 59 166 168 267 9.94e-13 70.5
Msa0659210 AT1G77740.1 40.500 200 110 3 60 259 77 267 5.52e-37 143
Msa0659210 AT1G77740.1 28.390 236 152 4 152 387 77 295 3.05e-20 93.6
Msa0659210 AT1G77740.1 35.185 108 62 1 59 166 168 267 9.94e-13 70.5
Msa0659210 AT2G41210.1 39.810 211 112 4 55 251 60 269 9.78e-37 142
Msa0659210 AT2G41210.1 30.964 197 112 1 104 300 68 240 1.76e-23 103
Msa0659210 AT2G41210.1 36.429 140 88 1 175 314 70 208 1.12e-16 82.4
Msa0659210 AT3G56960.2 39.500 200 111 3 60 250 72 270 6.96e-35 137
Msa0659210 AT3G56960.2 34.104 173 113 1 104 276 70 241 2.40e-28 117
Msa0659210 AT3G56960.2 32.515 163 109 1 152 314 72 233 8.33e-20 92.0
Msa0659210 AT3G56960.1 39.500 200 111 3 60 250 72 270 6.96e-35 137
Msa0659210 AT3G56960.1 34.104 173 113 1 104 276 70 241 2.40e-28 117
Msa0659210 AT3G56960.1 32.515 163 109 1 152 314 72 233 8.33e-20 92.0
Msa0659210 AT1G21920.2 36.066 183 116 1 100 282 168 349 2.52e-31 122
Msa0659210 AT1G21920.2 34.653 202 119 3 146 337 168 366 1.77e-27 112
Msa0659210 AT1G21920.2 33.663 202 110 1 58 259 172 349 6.76e-26 107
Msa0659210 AT1G21920.1 36.066 183 116 1 100 282 168 349 3.17e-31 123
Msa0659210 AT1G21920.1 34.673 199 117 3 146 334 168 363 3.10e-27 112
Msa0659210 AT1G21920.1 33.663 202 110 1 58 259 172 349 6.16e-26 108
Msa0659210 AT2G35170.1 30.597 268 172 5 39 296 152 415 3.39e-31 124
Msa0659210 AT2G35170.1 35.503 169 106 2 151 319 225 390 1.19e-22 100
Msa0659210 AT2G35170.1 31.579 114 78 0 187 300 214 327 3.98e-11 65.1
Msa0659210 AT4G17080.1 35.961 203 123 3 82 282 235 432 7.84e-31 124
Msa0659210 AT4G17080.1 38.150 173 106 1 59 231 256 427 2.82e-28 116
Msa0659210 AT4G17080.1 35.754 179 112 2 139 317 244 419 2.62e-25 107
Msa0659210 AT4G17080.1 30.952 126 87 0 60 185 303 428 2.80e-16 80.9
Msa0659210 AT4G17080.1 33.333 111 74 0 56 166 322 432 1.08e-11 66.6
Msa0659210 AT1G77660.1 36.364 176 111 1 100 275 172 346 1.24e-29 119
Msa0659210 AT1G77660.1 33.182 220 139 3 21 236 138 353 4.33e-28 114
Msa0659210 AT1G77660.1 38.710 155 94 1 146 300 172 325 2.10e-23 101
Msa0659210 AT2G26420.1 43.038 158 89 1 61 218 54 210 1.58e-28 118
Msa0659210 AT2G26420.1 35.404 161 103 1 104 264 51 210 4.36e-23 102
Msa0659210 AT2G26420.1 35.211 142 91 1 57 198 73 213 1.19e-20 94.7
Msa0659210 AT2G26420.1 31.677 161 109 1 153 313 54 213 1.11e-17 85.5
Msa0659210 AT2G26420.1 35.507 138 66 1 200 314 54 191 4.23e-16 80.9
Msa0659210 AT2G26420.2 43.038 158 89 1 61 218 54 210 1.83e-28 118
Msa0659210 AT2G26420.2 35.404 161 103 1 104 264 51 210 5.13e-23 101
Msa0659210 AT2G26420.2 35.211 142 91 1 57 198 73 213 1.40e-20 94.4
Msa0659210 AT2G26420.2 31.677 161 109 1 153 313 54 213 1.33e-17 85.5
Msa0659210 AT2G26420.2 35.507 138 66 1 200 314 54 191 4.89e-16 80.5
Msa0659210 AT2G26420.3 43.038 158 89 1 61 218 54 210 1.83e-28 118
Msa0659210 AT2G26420.3 35.404 161 103 1 104 264 51 210 5.13e-23 101
Msa0659210 AT2G26420.3 35.211 142 91 1 57 198 73 213 1.40e-20 94.4
Msa0659210 AT2G26420.3 31.677 161 109 1 153 313 54 213 1.33e-17 85.5
Msa0659210 AT2G26420.3 35.507 138 66 1 200 314 54 191 4.89e-16 80.5

Find 114 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATGATGGGAGAGGGATTT+TGG 0.132408 4_3:-69501950 Msa0659210:CDS
CGGTTGCCGGAGTTGATTTA+TGG 0.180495 4_3:+69504158 None:intergenic
TACCTCCATCCTTCCAAGTT+TGG 0.255059 4_3:+69502142 None:intergenic
GATTCAGATAATAGCTTTGA+TGG 0.263410 4_3:+69501342 None:intergenic
TAAAGACGAGATCGTGTATA+TGG 0.267545 4_3:-69501378 Msa0659210:CDS
ACCTCCATCCTTCCAAGTTT+GGG 0.269330 4_3:+69502143 None:intergenic
GGGATGTCTACACTGGAAAT+TGG 0.295174 4_3:-69501987 Msa0659210:CDS
TTCTTCACTTCGTCGGTTGC+CGG 0.302924 4_3:+69504145 None:intergenic
TTCAAATAAAGCTGAGTCTA+AGG 0.307919 4_3:-69501539 Msa0659210:CDS
AATGGACTTATACATGGATT+TGG 0.320121 4_3:-69501751 Msa0659210:CDS
GGGATGTCTACATCGGAAAT+TGG 0.328664 4_3:-69501846 Msa0659210:CDS
GAGGTAGCTACACTGGAAAT+TGG 0.329352 4_3:-69502125 Msa0659210:CDS
CAGAGAAAGGACTGATGATA+TGG 0.336491 4_3:-69501600 Msa0659210:CDS
TACGAGGGTGATTGGGTTGA+TGG 0.337164 4_3:-69502255 Msa0659210:CDS
CCTTGTCGCCGGTGGATTTA+CGG 0.350069 4_3:+69504212 None:intergenic
GCTAATGGGGATGTCGATAT+TGG 0.363580 4_3:-69501715 Msa0659210:CDS
GAGTTTATAGATATGCTAAT+GGG 0.365013 4_3:-69501729 Msa0659210:CDS
AATGGACTTGTACATGGATA+TGG 0.366109 4_3:-69502030 Msa0659210:CDS
GGAGTTTATAGATATGCTAA+TGG 0.368674 4_3:-69501730 Msa0659210:CDS
AATGGACTTAGACATGGATT+TGG 0.376853 4_3:-69501889 Msa0659210:CDS
GAGTTTATAGATTTGCAAAT+GGG 0.384860 4_3:-69501867 Msa0659210:CDS
GATCAATATACGAGGGTGAT+TGG 0.385479 4_3:-69502263 Msa0659210:CDS
GAACAATATACGAGGGTGAT+TGG 0.386941 4_3:-69501639 Msa0659210:CDS
GGTTGCCGGAGTTGATTTAT+GGG 0.389181 4_3:+69504159 None:intergenic
AGTTGATTTATGGGTGGCTT+TGG 0.393637 4_3:+69504168 None:intergenic
GGAGTTTATAAATCTGCAAA+TGG 0.399701 4_3:-69502009 Msa0659210:CDS
GGCATCTGGTGTGAGCCCTT+TGG 0.401200 4_3:-69501518 Msa0659210:CDS
AGAAAATACACATGCTCAAA+TGG 0.401828 4_3:-69501661 Msa0659210:CDS
TTTGATGGTTGTTGGTCAAA+TGG 0.406882 4_3:-69502048 Msa0659210:CDS
AAAGACAACATGGATGGAAC+AGG 0.409911 4_3:-69501820 Msa0659210:CDS
TTTGATGGTTGTTTGTCAAA+TGG 0.417735 4_3:-69501907 Msa0659210:CDS
TTCGATGGTTGTTGGTCAAA+TGG 0.420416 4_3:-69501769 Msa0659210:CDS
GGGACTGCGACTTGGGCTAA+TGG 0.422911 4_3:-69502078 Msa0659210:CDS
TTGGTGGCCAGCAGCTTATG+TGG 0.434386 4_3:+69504187 None:intergenic
GGAGTTTATAGATTTGCAAA+TGG 0.437498 4_3:-69501868 Msa0659210:CDS
GAGTTTATAAATCTGCAAAT+GGG 0.440314 4_3:-69502008 Msa0659210:CDS
AGATGTTCTACTATTCAAAC+GGG 0.442897 4_3:-69502353 Msa0659210:CDS
TGATTTATGGGTGGCTTTGG+TGG 0.443228 4_3:+69504171 None:intergenic
GGATTTACGGTCAGTTTGCT+TGG 0.448178 4_3:+69504225 None:intergenic
CGGAAAAGGGACTGCGACTT+GGG 0.448804 4_3:-69502085 Msa0659210:CDS
AACAATATACGAGGGTGATT+GGG 0.451603 4_3:-69501638 Msa0659210:CDS
ATGGCAATGGCACCCAAACT+TGG 0.453922 4_3:-69502155 Msa0659210:CDS
AAAGCTGAGTCTAAGGCATC+TGG 0.456725 4_3:-69501532 Msa0659210:CDS
AGGTAGCTACACTGGAAATT+GGG 0.461160 4_3:-69502124 Msa0659210:CDS
ATGTCGATATTGGTAACTTC+AGG 0.462222 4_3:-69501705 Msa0659210:CDS
GAGATGTTCTACTATTCAAA+CGG 0.464322 4_3:-69502354 Msa0659210:CDS
ATCAATATACGAGGGTGATT+GGG 0.465727 4_3:-69502262 Msa0659210:CDS
GTGTGGCTTCTTCACTTCGT+CGG 0.472255 4_3:+69504138 None:intergenic
TGGAACAGGGATTATGAGTT+GGG 0.472804 4_3:-69501806 Msa0659210:CDS
ATTTGAAATCTGCAATGAAG+TGG 0.473202 4_3:+69501555 None:intergenic
TGGTCAAATGGACTTATACA+TGG 0.474958 4_3:-69501757 Msa0659210:CDS
AAAATAGACAGAATGGATGA+TGG 0.482430 4_3:-69501964 Msa0659210:CDS
GACAGAATGGATGATGGGAG+AGG 0.485525 4_3:-69501958 Msa0659210:CDS
TGTCTATGTTGGTAACTTCA+AGG 0.486468 4_3:-69502328 Msa0659210:CDS
ACGGAAAAGGGACTGCGACT+TGG 0.489434 4_3:-69502086 Msa0659210:CDS
TGCAATGAAGTGGAACCATC+AGG 0.492197 4_3:+69501565 None:intergenic
CAAGGCTCCACATAAGCTGC+TGG 0.492954 4_3:-69504194 Msa0659210:CDS
GAGCCCTTTGGTTCTCGAAA+GGG 0.502856 4_3:-69501506 Msa0659210:CDS
GGACTGCTCATACAATCATC+AGG 0.504986 4_3:-69502216 Msa0659210:CDS
TGGTCAAATGGACTTGTACA+TGG 0.509652 4_3:-69502036 Msa0659210:CDS
GCGATGTCTTCGATGGTTGT+TGG 0.515994 4_3:-69501777 Msa0659210:CDS
CATCGTTTCTTGTAAACTGT+AGG 0.517692 4_3:-69502383 Msa0659210:CDS
TGGGTTGATGGAGAGATGAC+AGG 0.520799 4_3:-69502243 Msa0659210:CDS
GGGATTATGAGTTGGGCTAC+CGG 0.525267 4_3:-69501799 Msa0659210:CDS
ACAGAATGGATGATGGGAGA+GGG 0.526125 4_3:-69501957 Msa0659210:CDS
AAAGACGAGATCGTGTATAT+GGG 0.526778 4_3:-69501377 Msa0659210:CDS
ATGGAACAGGGATTATGAGT+TGG 0.528516 4_3:-69501807 Msa0659210:CDS
GAGAATTGTCGTCATGGCAA+TGG 0.529534 4_3:-69502168 Msa0659210:CDS
AGAGAAAGGACTGATGATAT+GGG 0.534627 4_3:-69501599 Msa0659210:CDS
TTCTTTGAGAATTGTCGTCA+TGG 0.535373 4_3:-69502174 Msa0659210:CDS
CCGTAAATCCACCGGCGACA+AGG 0.536931 4_3:-69504212 Msa0659210:CDS
GGCACCCAAACTTGGAAGGA+TGG 0.545646 4_3:-69502147 Msa0659210:CDS
AAATAGACAGAATGGATGAT+GGG 0.549899 4_3:-69501963 Msa0659210:CDS
TGAGCCCTTTGGTTCTCGAA+AGG 0.553188 4_3:-69501507 Msa0659210:CDS
GGAAAGTACACATGGTCAGA+TGG 0.558174 4_3:-69502285 Msa0659210:CDS
CTTATGTGGAGCCTTGTCGC+CGG 0.558415 4_3:+69504201 None:intergenic
ATGTGGAGCCTTGTCGCCGG+TGG 0.562717 4_3:+69504204 None:intergenic
TTGTCAAATGGACTTAGACA+TGG 0.568143 4_3:-69501895 Msa0659210:CDS
AGTTTATAGATTTGCAAATG+GGG 0.568661 4_3:-69501866 Msa0659210:CDS
GCAAATGGGGATGTCTACAC+TGG 0.570058 4_3:-69501994 Msa0659210:CDS
AAATTGGAAAATAGACAGAA+TGG 0.571082 4_3:-69501971 Msa0659210:CDS
TCAAACGGGGATGTCTATGT+TGG 0.574532 4_3:-69502339 Msa0659210:CDS
GCTACCGGCGATGTCTTCGA+TGG 0.576597 4_3:-69501784 Msa0659210:CDS
TTCAGGAGTAAACTTCTCCA+TGG 0.576629 4_3:-69501688 Msa0659210:CDS
AAGGATGGAGGTAGCTACAC+TGG 0.576669 4_3:-69502132 Msa0659210:CDS
GATGGAGAGATGACAGGGAA+AGG 0.581963 4_3:-69502237 Msa0659210:CDS
TGCCGGAGTTGATTTATGGG+TGG 0.582432 4_3:+69504162 None:intergenic
TATTCCCTTTCGAGAACCAA+AGG 0.582961 4_3:+69501502 None:intergenic
ATTCCCTTTCGAGAACCAAA+GGG 0.584361 4_3:+69501503 None:intergenic
TGGATAAAAGACAACATGGA+TGG 0.584838 4_3:-69501826 Msa0659210:CDS
CAATGGCACCCAAACTTGGA+AGG 0.585341 4_3:-69502151 Msa0659210:CDS
TTCAAGGATGAACTTCCTCA+TGG 0.590492 4_3:-69502312 Msa0659210:CDS
GATGAACTTCCTCATGGCAA+AGG 0.593188 4_3:-69502306 Msa0659210:CDS
AGCCACCCATAAATCAACTC+CGG 0.601961 4_3:-69504164 Msa0659210:CDS
CTCGAAAGGGAATATATGCA+AGG 0.605315 4_3:-69501493 Msa0659210:CDS
ACAACCATCGAAGACATCGC+CGG 0.607359 4_3:+69501780 None:intergenic
GGGTTGATGGAGAGATGACA+GGG 0.608966 4_3:-69502242 Msa0659210:CDS
TGGGCCCTTCAAAATCCTGA+TGG 0.617949 4_3:-69501580 Msa0659210:CDS
AGTTTATAGATATGCTAATG+GGG 0.619790 4_3:-69501728 Msa0659210:CDS
AGTTTATAAATCTGCAAATG+GGG 0.626243 4_3:-69502007 Msa0659210:CDS
CTCAAATGGAACAATATACG+AGG 0.634118 4_3:-69501647 Msa0659210:CDS
GTGTACTTTCCTTTGCCATG+AGG 0.651733 4_3:+69502297 None:intergenic
CAAACTGACCGTAAATCCAC+CGG 0.652448 4_3:-69504220 Msa0659210:CDS
GCAAATGGGGATGTCTACAT+CGG 0.652522 4_3:-69501853 Msa0659210:CDS
AAATTGGATAAAAGACAACA+TGG 0.660956 4_3:-69501830 Msa0659210:CDS
AAGACAACATGGATGGAACA+GGG 0.663275 4_3:-69501819 Msa0659210:CDS
ACCCAAACTTGGAAGGATGG+AGG 0.668025 4_3:-69502144 Msa0659210:CDS
GTCAGATGGATCAATATACG+AGG 0.688571 4_3:-69502271 Msa0659210:CDS
ATGGCAAAGGAAAGTACACA+TGG 0.689912 4_3:-69502293 Msa0659210:CDS
TCAAATGGAACAATATACGA+GGG 0.706226 4_3:-69501646 Msa0659210:CDS
TCAGATGGATCAATATACGA+GGG 0.726714 4_3:-69502270 Msa0659210:CDS
ATCATCAGGAGAAATGTACG+AGG 0.734911 4_3:-69502202 Msa0659210:CDS
TCATCAGGAGAAATGTACGA+GGG 0.736398 4_3:-69502201 Msa0659210:CDS
GATGTTCTACTATTCAAACG+GGG 0.785195 4_3:-69502352 Msa0659210:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATAAGTGAGTAAATAT+TGG - chr4_3:69501835-69501854 Msa0659210:CDS 15.0%
!! ATAAAAGAGAGTAATTTAAT+AGG + chr4_3:69501906-69501925 None:intergenic 15.0%
!! ATAACAAAAATCTGTTTAAA+TGG + chr4_3:69502633-69502652 None:intergenic 15.0%
!! TATTTCATACTAGTAATTTA+GGG + chr4_3:69502720-69502739 None:intergenic 15.0%
!! ATATTTCATACTAGTAATTT+AGG + chr4_3:69502721-69502740 None:intergenic 15.0%
!! TAGTATGAAATATAAATAGA+GGG - chr4_3:69502728-69502747 Msa0659210:intron 15.0%
!!! TGTTTTGATATTTCTTTCAA+TGG + chr4_3:69501769-69501788 None:intergenic 20.0%
!! ACATTAGAGAGTTTATATTA+CGG - chr4_3:69501786-69501805 Msa0659210:CDS 20.0%
!! TTGAAACTCCATTTATATTT+CGG + chr4_3:69502080-69502099 None:intergenic 20.0%
!! CGAAATTAAAAAAAACCAAA+TGG - chr4_3:69502201-69502220 Msa0659210:CDS 20.0%
!!! GATAATCACTTTTTTTTTTG+TGG - chr4_3:69502290-69502309 Msa0659210:CDS 20.0%
!! TAATATGCATAATATATGCA+AGG + chr4_3:69502335-69502354 None:intergenic 20.0%
!! CTAGTATGAAATATAAATAG+AGG - chr4_3:69502727-69502746 Msa0659210:intron 20.0%
!!! TAGTATTATTTTCGATTTTG+TGG - chr4_3:69502940-69502959 Msa0659210:intron 20.0%
!!! TATTGTAGTTTGTAAATGAA+TGG + chr4_3:69503126-69503145 None:intergenic 20.0%
!! TAATAACAAGAACAAAAAAC+AGG - chr4_3:69504158-69504177 Msa0659210:CDS 20.0%
! TGAAATCTCTCAAAATTCAA+AGG - chr4_3:69501563-69501582 Msa0659210:CDS 25.0%
!!! TGTTTTAAGTTGAGTATTAG+AGG - chr4_3:69501653-69501672 Msa0659210:CDS 25.0%
!!! GTTTTAAGTTGAGTATTAGA+GGG - chr4_3:69501654-69501673 Msa0659210:CDS 25.0%
! AGTATTAGAGGGAATTAAAA+GGG - chr4_3:69501665-69501684 Msa0659210:CDS 25.0%
! ATTTATCAAACTGATGAGAT+AGG + chr4_3:69501821-69501840 None:intergenic 25.0%
!! TTGGAACAAAAACAAGTTTT+AGG - chr4_3:69501854-69501873 Msa0659210:CDS 25.0%
! TTACAAATCATCATTTGACA+CGG + chr4_3:69501931-69501950 None:intergenic 25.0%
!!! GTTTGAAATGATTTGTTAGA+AGG - chr4_3:69501959-69501978 Msa0659210:CDS 25.0%
!!! AATGATTTGTTAGAAGGATA+TGG - chr4_3:69501965-69501984 Msa0659210:CDS 25.0%
! ATAGATACCCGAAATATAAA+TGG - chr4_3:69502069-69502088 Msa0659210:CDS 25.0%
! TGAAACTCCATTTATATTTC+GGG + chr4_3:69502079-69502098 None:intergenic 25.0%
! TCAAAAAAAAAATTGTGCAG+CGG - chr4_3:69502096-69502115 Msa0659210:CDS 25.0%
!!! TTTAATTTGACTTGACCATT+TGG + chr4_3:69502219-69502238 None:intergenic 25.0%
!!! GTTTCTTTTAAAAGCGATTT+AGG + chr4_3:69502694-69502713 None:intergenic 25.0%
! GATTAAAAGAATCGAAAAGT+CGG - chr4_3:69502797-69502816 Msa0659210:intron 25.0%
! TGGGAAAAAGATAAAAAAGA+CGG - chr4_3:69503475-69503494 Msa0659210:intron 25.0%
! AAAGATAAAAAAGACGGAAA+AGG - chr4_3:69503481-69503500 Msa0659210:intron 25.0%
! AAGATAAAAAAGACGGAAAA+GGG - chr4_3:69503482-69503501 Msa0659210:intron 25.0%
! GAGTTTATAAATCTGCAAAT+GGG - chr4_3:69503572-69503591 Msa0659210:intron 25.0%
! AGTTTATAAATCTGCAAATG+GGG - chr4_3:69503573-69503592 Msa0659210:intron 25.0%
! AAATTGGAAAATAGACAGAA+TGG - chr4_3:69503609-69503628 Msa0659210:intron 25.0%
! GAGTTTATAGATTTGCAAAT+GGG - chr4_3:69503713-69503732 Msa0659210:intron 25.0%
! AGTTTATAGATTTGCAAATG+GGG - chr4_3:69503714-69503733 Msa0659210:intron 25.0%
! AAATTGGATAAAAGACAACA+TGG - chr4_3:69503750-69503769 Msa0659210:intron 25.0%
! GAGTTTATAGATATGCTAAT+GGG - chr4_3:69503851-69503870 Msa0659210:intron 25.0%
! AGTTTATAGATATGCTAATG+GGG - chr4_3:69503852-69503871 Msa0659210:intron 25.0%
!!! ACGTTGGAATGGAATTTTTT+GGG + chr4_3:69501535-69501554 None:intergenic 30.0%
GAGTATTAGAGGGAATTAAA+AGG - chr4_3:69501664-69501683 Msa0659210:CDS 30.0%
AGTCACATAAATTTGCAGAA+TGG - chr4_3:69501728-69501747 Msa0659210:CDS 30.0%
GTCACATAAATTTGCAGAAT+GGG - chr4_3:69501729-69501748 Msa0659210:CDS 30.0%
TACAAATCATCATTTGACAC+GGG + chr4_3:69501930-69501949 None:intergenic 30.0%
GGATATGGTGATATGATTTA+TGG - chr4_3:69501980-69501999 Msa0659210:CDS 30.0%
TGGTTACGATATATGTAGAA+TGG - chr4_3:69502042-69502061 Msa0659210:CDS 30.0%
TAACTTTGTTGCAAAAAGTG+AGG - chr4_3:69502485-69502504 Msa0659210:intron 30.0%
! TGAGATTTTGTGTGATTGTT+TGG - chr4_3:69502523-69502542 Msa0659210:intron 30.0%
!! TCTTTTAAAAGCGATTTAGG+TGG + chr4_3:69502691-69502710 None:intergenic 30.0%
TTCTAAGTGCTAACAAACAA+GGG + chr4_3:69503060-69503079 None:intergenic 30.0%
ATTCTAAGTGCTAACAAACA+AGG + chr4_3:69503061-69503080 None:intergenic 30.0%
GAGATGTTCTACTATTCAAA+CGG - chr4_3:69503226-69503245 Msa0659210:intron 30.0%
AGATGTTCTACTATTCAAAC+GGG - chr4_3:69503227-69503246 Msa0659210:intron 30.0%
GGAGTTTATAAATCTGCAAA+TGG - chr4_3:69503571-69503590 Msa0659210:intron 30.0%
AAAATAGACAGAATGGATGA+TGG - chr4_3:69503616-69503635 Msa0659210:intron 30.0%
AAATAGACAGAATGGATGAT+GGG - chr4_3:69503617-69503636 Msa0659210:intron 30.0%
! TTTGATGGTTGTTTGTCAAA+TGG - chr4_3:69503673-69503692 Msa0659210:intron 30.0%
GGAGTTTATAGATTTGCAAA+TGG - chr4_3:69503712-69503731 Msa0659210:intron 30.0%
! AATGGACTTATACATGGATT+TGG - chr4_3:69503829-69503848 Msa0659210:intron 30.0%
GGAGTTTATAGATATGCTAA+TGG - chr4_3:69503850-69503869 Msa0659210:intron 30.0%
AGAAAATACACATGCTCAAA+TGG - chr4_3:69503919-69503938 Msa0659210:intron 30.0%
TCAAATGGAACAATATACGA+GGG - chr4_3:69503934-69503953 Msa0659210:intron 30.0%
GATAAAAAAGTGACAGAGAA+AGG - chr4_3:69503967-69503986 Msa0659210:intron 30.0%
! ATTTGAAATCTGCAATGAAG+TGG + chr4_3:69504028-69504047 None:intergenic 30.0%
TTCAAATAAAGCTGAGTCTA+AGG - chr4_3:69504041-69504060 Msa0659210:intron 30.0%
ATTCAAGGTTTGAATCTGTG+TGG + chr4_3:69501462-69501481 None:intergenic 35.0%
TCTCTCAAAGCAACAATTCA+AGG + chr4_3:69501477-69501496 None:intergenic 35.0%
!!! GACGTTGGAATGGAATTTTT+TGG + chr4_3:69501536-69501555 None:intergenic 35.0%
CTTGTTAGATCGATAGAGTA+TGG - chr4_3:69502022-69502041 Msa0659210:CDS 35.0%
! GATTTTGTGTGATTGTTTGG+TGG - chr4_3:69502526-69502545 Msa0659210:intron 35.0%
TAAAAGAATCGAAAAGTCGG+AGG - chr4_3:69502800-69502819 Msa0659210:intron 35.0%
AGAAGTTTACCAGATTGCTT+AGG + chr4_3:69502860-69502879 None:intergenic 35.0%
CATCTATCTGCATATGTGAT+GGG - chr4_3:69503015-69503034 Msa0659210:intron 35.0%
!! TGCATATGTGATGGGTTTAT+CGG - chr4_3:69503023-69503042 Msa0659210:intron 35.0%
CATCGTTTCTTGTAAACTGT+AGG - chr4_3:69503197-69503216 Msa0659210:intron 35.0%
GATGTTCTACTATTCAAACG+GGG - chr4_3:69503228-69503247 Msa0659210:intron 35.0%
!! TGTCTATGTTGGTAACTTCA+AGG - chr4_3:69503252-69503271 Msa0659210:intron 35.0%
TCAGATGGATCAATATACGA+GGG - chr4_3:69503310-69503329 Msa0659210:intron 35.0%
ATCAATATACGAGGGTGATT+GGG - chr4_3:69503318-69503337 Msa0659210:intron 35.0%
! TTCTTTGAGAATTGTCGTCA+TGG - chr4_3:69503406-69503425 Msa0659210:intron 35.0%
!! GCTAATGGTGATGTTTTTGA+TGG - chr4_3:69503517-69503536 Msa0659210:intron 35.0%
!! GTGATGTTTTTGATGGTTGT+TGG - chr4_3:69503524-69503543 Msa0659210:intron 35.0%
! TTTGATGGTTGTTGGTCAAA+TGG - chr4_3:69503532-69503551 Msa0659210:intron 35.0%
! AATGGACTTGTACATGGATA+TGG - chr4_3:69503550-69503569 Msa0659210:intron 35.0%
!! GCTAATGGTGATGTTTTTGA+TGG - chr4_3:69503517-69503536 Msa0659210:intron 35.0%
! TTGTCAAATGGACTTAGACA+TGG - chr4_3:69503685-69503704 Msa0659210:intron 35.0%
! AATGGACTTAGACATGGATT+TGG - chr4_3:69503691-69503710 Msa0659210:intron 35.0%
TGGATAAAAGACAACATGGA+TGG - chr4_3:69503754-69503773 Msa0659210:intron 35.0%
! TGGTCAAATGGACTTATACA+TGG - chr4_3:69503823-69503842 Msa0659210:intron 35.0%
! ATGTCGATATTGGTAACTTC+AGG - chr4_3:69503875-69503894 Msa0659210:intron 35.0%
! ATGTGTATTTTCTGTTGCCA+TGG + chr4_3:69503912-69503931 None:intergenic 35.0%
CTCAAATGGAACAATATACG+AGG - chr4_3:69503933-69503952 Msa0659210:intron 35.0%
AACAATATACGAGGGTGATT+GGG - chr4_3:69503942-69503961 Msa0659210:intron 35.0%
AGAGAAAGGACTGATGATAT+GGG - chr4_3:69503981-69504000 Msa0659210:intron 35.0%
TAAAGACGAGATCGTGTATA+TGG - chr4_3:69504202-69504221 Msa0659210:CDS 35.0%
AAAGACGAGATCGTGTATAT+GGG - chr4_3:69504203-69504222 Msa0659210:CDS 35.0%
! AGTTGATTTATGGGTGGCTT+TGG + chr4_3:69501415-69501434 None:intergenic 40.0%
AGATTTCACGAACAAGACGT+TGG + chr4_3:69501551-69501570 None:intergenic 40.0%
ATGACTAGAAATAGAGCGAG+TGG - chr4_3:69501695-69501714 Msa0659210:CDS 40.0%
TGACTAGAAATAGAGCGAGT+GGG - chr4_3:69501696-69501715 Msa0659210:CDS 40.0%
TTTGCAGAATGGGTCATTGA+AGG - chr4_3:69501739-69501758 Msa0659210:CDS 40.0%
TTGCAGAATGGGTCATTGAA+GGG - chr4_3:69501740-69501759 Msa0659210:CDS 40.0%
AAAATTGTGCAGCGGAATTG+TGG - chr4_3:69502104-69502123 Msa0659210:CDS 40.0%
CATAATATATGCAAGGTCCG+AGG + chr4_3:69502328-69502347 None:intergenic 40.0%
AAATTCATGCTCTTGTGTGC+TGG - chr4_3:69502986-69503005 Msa0659210:intron 40.0%
GCATCTATCTGCATATGTGA+TGG - chr4_3:69503014-69503033 Msa0659210:intron 40.0%
TTCAAGGATGAACTTCCTCA+TGG - chr4_3:69503268-69503287 Msa0659210:intron 40.0%
! ATGGCAAAGGAAAGTACACA+TGG - chr4_3:69503287-69503306 Msa0659210:intron 40.0%
GTCAGATGGATCAATATACG+AGG - chr4_3:69503309-69503328 Msa0659210:intron 40.0%
GATCAATATACGAGGGTGAT+TGG - chr4_3:69503317-69503336 Msa0659210:intron 40.0%
ATCATCAGGAGAAATGTACG+AGG - chr4_3:69503378-69503397 Msa0659210:intron 40.0%
TCATCAGGAGAAATGTACGA+GGG - chr4_3:69503379-69503398 Msa0659210:intron 40.0%
AGGTAGCTACACTGGAAATT+GGG - chr4_3:69503456-69503475 Msa0659210:intron 40.0%
! TGGTCAAATGGACTTGTACA+TGG - chr4_3:69503544-69503563 Msa0659210:intron 40.0%
AAAGACAACATGGATGGAAC+AGG - chr4_3:69503760-69503779 Msa0659210:intron 40.0%
AAGACAACATGGATGGAACA+GGG - chr4_3:69503761-69503780 Msa0659210:intron 40.0%
ATGGAACAGGGATTATGAGT+TGG - chr4_3:69503773-69503792 Msa0659210:intron 40.0%
TGGAACAGGGATTATGAGTT+GGG - chr4_3:69503774-69503793 Msa0659210:intron 40.0%
TTCGATGGTTGTTGGTCAAA+TGG - chr4_3:69503811-69503830 Msa0659210:intron 40.0%
TTCAGGAGTAAACTTCTCCA+TGG - chr4_3:69503892-69503911 Msa0659210:intron 40.0%
GAACAATATACGAGGGTGAT+TGG - chr4_3:69503941-69503960 Msa0659210:intron 40.0%
CAGAGAAAGGACTGATGATA+TGG - chr4_3:69503980-69503999 Msa0659210:intron 40.0%
! GGAACCATCAGGATTTTGAA+GGG + chr4_3:69504007-69504026 None:intergenic 40.0%
! TGGAACCATCAGGATTTTGA+AGG + chr4_3:69504008-69504027 None:intergenic 40.0%
ATTCCCTTTCGAGAACCAAA+GGG + chr4_3:69504080-69504099 None:intergenic 40.0%
TATTCCCTTTCGAGAACCAA+AGG + chr4_3:69504081-69504100 None:intergenic 40.0%
CTCGAAAGGGAATATATGCA+AGG - chr4_3:69504087-69504106 Msa0659210:intron 40.0%
! GGATTTACGGTCAGTTTGCT+TGG + chr4_3:69501358-69501377 None:intergenic 45.0%
CAAACTGACCGTAAATCCAC+CGG - chr4_3:69501360-69501379 Msa0659210:CDS 45.0%
!! TGATTTATGGGTGGCTTTGG+TGG + chr4_3:69501412-69501431 None:intergenic 45.0%
AGCCACCCATAAATCAACTC+CGG - chr4_3:69501416-69501435 Msa0659210:CDS 45.0%
! GGTTGCCGGAGTTGATTTAT+GGG + chr4_3:69501424-69501443 None:intergenic 45.0%
TCACGAACAAGACGTTGGAA+TGG + chr4_3:69501546-69501565 None:intergenic 45.0%
AGAAATAGAGCGAGTGGGAA+AGG - chr4_3:69501701-69501720 Msa0659210:CDS 45.0%
TCATGTGAGCTTAGCTCAGT+TGG + chr4_3:69502364-69502383 None:intergenic 45.0%
AAGAATCGAAAAGTCGGAGG+AGG - chr4_3:69502803-69502822 Msa0659210:intron 45.0%
AAAAGTCGGAGGAGGAGAAA+AGG - chr4_3:69502811-69502830 Msa0659210:intron 45.0%
TCAAACGGGGATGTCTATGT+TGG - chr4_3:69503241-69503260 Msa0659210:intron 45.0%
GATGAACTTCCTCATGGCAA+AGG - chr4_3:69503274-69503293 Msa0659210:intron 45.0%
GTGTACTTTCCTTTGCCATG+AGG + chr4_3:69503286-69503305 None:intergenic 45.0%
! GGAAAGTACACATGGTCAGA+TGG - chr4_3:69503295-69503314 Msa0659210:intron 45.0%
GGACTGCTCATACAATCATC+AGG - chr4_3:69503364-69503383 Msa0659210:intron 45.0%
GAGAATTGTCGTCATGGCAA+TGG - chr4_3:69503412-69503431 Msa0659210:intron 45.0%
ACCTCCATCCTTCCAAGTTT+GGG + chr4_3:69503440-69503459 None:intergenic 45.0%
TACCTCCATCCTTCCAAGTT+TGG + chr4_3:69503441-69503460 None:intergenic 45.0%
GAGGTAGCTACACTGGAAAT+TGG - chr4_3:69503455-69503474 Msa0659210:intron 45.0%
GGGATGTCTACACTGGAAAT+TGG - chr4_3:69503593-69503612 Msa0659210:intron 45.0%
!! ACAGAATGGATGATGGGAGA+GGG - chr4_3:69503623-69503642 Msa0659210:intron 45.0%
! ATGGGAGAGGGATTTTGGAT+TGG - chr4_3:69503635-69503654 Msa0659210:intron 45.0%
! TGGGAGAGGGATTTTGGATT+GGG - chr4_3:69503636-69503655 Msa0659210:intron 45.0%
!! GGGATTTTGGATTGGGCTAA+TGG - chr4_3:69503643-69503662 Msa0659210:intron 45.0%
GCAAATGGGGATGTCTACAT+CGG - chr4_3:69503727-69503746 Msa0659210:intron 45.0%
GGGATGTCTACATCGGAAAT+TGG - chr4_3:69503734-69503753 Msa0659210:intron 45.0%
GCTAATGGGGATGTCGATAT+TGG - chr4_3:69503865-69503884 Msa0659210:intron 45.0%
TGCAATGAAGTGGAACCATC+AGG + chr4_3:69504018-69504037 None:intergenic 45.0%
! AAAGCTGAGTCTAAGGCATC+TGG - chr4_3:69504048-69504067 Msa0659210:intron 45.0%
!! ATTATAATAATAATAATAGA+TGG + chr4_3:69503175-69503194 None:intergenic 5.0%
! TGCCGGAGTTGATTTATGGG+TGG + chr4_3:69501421-69501440 None:intergenic 50.0%
! CGGTTGCCGGAGTTGATTTA+TGG + chr4_3:69501425-69501444 None:intergenic 50.0%
! TTCTTCACTTCGTCGGTTGC+CGG + chr4_3:69501438-69501457 None:intergenic 50.0%
GTGTGGCTTCTTCACTTCGT+CGG + chr4_3:69501445-69501464 None:intergenic 50.0%
TGTGGCCTGAGTTTGAACCT+CGG - chr4_3:69502308-69502327 Msa0659210:CDS 50.0%
AAGGTCCGAGGTTCAAACTC+AGG + chr4_3:69502316-69502335 None:intergenic 50.0%
GGAGGAGGAGAAAAGGAGAT+CGG - chr4_3:69502818-69502837 Msa0659210:intron 50.0%
GAGGAGGAGAAAAGGAGATC+GGG - chr4_3:69502819-69502838 Msa0659210:intron 50.0%
GAGATCGGGAAAAACGAACG+CGG - chr4_3:69502833-69502852 Msa0659210:intron 50.0%
! ATGCTCTTGTGTGCTGGTAG+AGG - chr4_3:69502992-69503011 Msa0659210:intron 50.0%
!! TACGAGGGTGATTGGGTTGA+TGG - chr4_3:69503325-69503344 Msa0659210:intron 50.0%
!! TGGGTTGATGGAGAGATGAC+AGG - chr4_3:69503337-69503356 Msa0659210:intron 50.0%
!! GGGTTGATGGAGAGATGACA+GGG - chr4_3:69503338-69503357 Msa0659210:intron 50.0%
GATGGAGAGATGACAGGGAA+AGG - chr4_3:69503343-69503362 Msa0659210:intron 50.0%
ATGGCAATGGCACCCAAACT+TGG - chr4_3:69503425-69503444 Msa0659210:intron 50.0%
CAATGGCACCCAAACTTGGA+AGG - chr4_3:69503429-69503448 Msa0659210:intron 50.0%
ACCCAAACTTGGAAGGATGG+AGG - chr4_3:69503436-69503455 Msa0659210:intron 50.0%
AAGGATGGAGGTAGCTACAC+TGG - chr4_3:69503448-69503467 Msa0659210:intron 50.0%
GCAAATGGGGATGTCTACAC+TGG - chr4_3:69503586-69503605 Msa0659210:intron 50.0%
!! GACAGAATGGATGATGGGAG+AGG - chr4_3:69503622-69503641 Msa0659210:intron 50.0%
!!! GGATGATGGGAGAGGGATTT+TGG - chr4_3:69503630-69503649 Msa0659210:intron 50.0%
! GGGATTATGAGTTGGGCTAC+CGG - chr4_3:69503781-69503800 Msa0659210:intron 50.0%
ACAACCATCGAAGACATCGC+CGG + chr4_3:69503803-69503822 None:intergenic 50.0%
GCGATGTCTTCGATGGTTGT+TGG - chr4_3:69503803-69503822 Msa0659210:intron 50.0%
TGGGCCCTTCAAAATCCTGA+TGG - chr4_3:69504000-69504019 Msa0659210:intron 50.0%
!! TGAGCCCTTTGGTTCTCGAA+AGG - chr4_3:69504073-69504092 Msa0659210:intron 50.0%
!! GAGCCCTTTGGTTCTCGAAA+GGG - chr4_3:69504074-69504093 Msa0659210:intron 50.0%
! CCTTGTCGCCGGTGGATTTA+CGG + chr4_3:69501371-69501390 None:intergenic 55.0%
! CTTATGTGGAGCCTTGTCGC+CGG + chr4_3:69501382-69501401 None:intergenic 55.0%
CAAGGCTCCACATAAGCTGC+TGG - chr4_3:69501386-69501405 Msa0659210:CDS 55.0%
! TTGGTGGCCAGCAGCTTATG+TGG + chr4_3:69501396-69501415 None:intergenic 55.0%
GAACGCGGACCTAAGCAATC+TGG - chr4_3:69502848-69502867 Msa0659210:intron 55.0%
GGCACCCAAACTTGGAAGGA+TGG - chr4_3:69503433-69503452 Msa0659210:intron 55.0%
ACGGAAAAGGGACTGCGACT+TGG - chr4_3:69503494-69503513 Msa0659210:intron 55.0%
CGGAAAAGGGACTGCGACTT+GGG - chr4_3:69503495-69503514 Msa0659210:intron 55.0%
CCGTAAATCCACCGGCGACA+AGG - chr4_3:69501368-69501387 Msa0659210:CDS 60.0%
! GGGACTGCGACTTGGGCTAA+TGG - chr4_3:69503502-69503521 Msa0659210:intron 60.0%
GCTACCGGCGATGTCTTCGA+TGG - chr4_3:69503796-69503815 Msa0659210:intron 60.0%
GGCATCTGGTGTGAGCCCTT+TGG - chr4_3:69504062-69504081 Msa0659210:intron 60.0%
! ATGTGGAGCCTTGTCGCCGG+TGG + chr4_3:69501379-69501398 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 69501345 69504257 69501345 ID=Msa0659210;Name=Msa0659210
chr4_3 mRNA 69501345 69504257 69501345 ID=Msa0659210-mRNA-1;Parent=Msa0659210;Name=Msa0659210-mRNA-1;_AED=0.25;_eAED=0.25;_QI=0|0|0|1|0|0|2|0|397
chr4_3 exon 69504153 69504257 69504153 ID=Msa0659210-mRNA-1:exon:20435;Parent=Msa0659210-mRNA-1
chr4_3 exon 69501345 69502433 69501345 ID=Msa0659210-mRNA-1:exon:20434;Parent=Msa0659210-mRNA-1
chr4_3 CDS 69504153 69504257 69504153 ID=Msa0659210-mRNA-1:cds;Parent=Msa0659210-mRNA-1
chr4_3 CDS 69501345 69502433 69501345 ID=Msa0659210-mRNA-1:cds;Parent=Msa0659210-mRNA-1
Gene Sequence

>Msa0659210

ATGGCTCGTACCAAGCAAACTGACCGTAAATCCACCGGCGACAAGGCTCCACATAAGCTGCTGGCCACCAAAGCCACCCATAAATCAACTCCGGCAACCGACGAATATCCATCTATTATTATTATTATAATCTCATCGTTTCTTGTAAACTGTAGGTTTAAAGAGATGTTCTACTATTCAAACGGGGATGTCTATGTTGGTAACTTCAAGGATGAACTTCCTCATGGCAAAGGAAAGTACACATGGTCAGATGGATCAATATACGAGGGTGATTGGGTTGATGGAGAGATGACAGGGAAAGGACTGCTCATACAATCATCAGGAGAAATGTACGAGGGTGAATTCTTTGAGAATTGTCGTCATGGCAATGGCACCCAAACTTGGAAGGATGGAGGTAGCTACACTGGAAATTGGGAAAAAGATAAAAAAGACGGAAAAGGGACTGCGACTTGGGCTAATGGTGATGTTTTTGATGGTTGTTGGTCAAATGGACTTGTACATGGATATGGAGTTTATAAATCTGCAAATGGGGATGTCTACACTGGAAATTGGAAAATAGACAGAATGGATGATGGGAGAGGGATTTTGGATTGGGCTAATGGTGATGTTTTTGATGGTTGTTTGTCAAATGGACTTAGACATGGATTTGGAGTTTATAGATTTGCAAATGGGGATGTCTACATCGGAAATTGGATAAAAGACAACATGGATGGAACAGGGATTATGAGTTGGGCTACCGGCGATGTCTTCGATGGTTGTTGGTCAAATGGACTTATACATGGATTTGGAGTTTATAGATATGCTAATGGGGATGTCGATATTGGTAACTTCAGGAGTAAACTTCTCCATGGCAACAGAAAATACACATGCTCAAATGGAACAATATACGAGGGTGATTGGGTTGATAAAAAAGTGACAGAGAAAGGACTGATGATATGGGCCCTTCAAAATCCTGATGGTTCCACTTCATTGCAGATTTCAAATAAAGCTGAGTCTAAGGCATCTGGTGTGAGCCCTTTGGTTCTCGAAAGGGAATATATGCAAGGAGTTCTAATTGTTGAGAAAATTATACAGTATTCAGAAGTAACACATAATAATAACAAGAACAAAAAACAGGATGCATTTAGTGTGAAGAAAGTAAAGACGAGATCGTGTATATGGGCATTTTTTAAGACCATCAAAGCTATTATCTGA
Protein sequence

>Msa0659210

MARTKQTDRKSTGDKAPHKLLATKATHKSTPATDEYPSIIIIIISSFLVNCRFKEMFYYSNGDVYVGNFKDELPHGKGKYTWSDGSIYEGDWVDGEMTGKGLLIQSSGEMYEGEFFENCRHGNGTQTWKDGGSYTGNWEKDKKDGKGTATWANGDVFDGCWSNGLVHGYGVYKSANGDVYTGNWKIDRMDDGRGILDWANGDVFDGCLSNGLRHGFGVYRFANGDVYIGNWIKDNMDGTGIMSWATGDVFDGCWSNGLIHGFGVYRYANGDVDIGNFRSKLLHGNRKYTCSNGTIYEGDWVDKKVTEKGLMIWALQNPDGSTSLQISNKAESKASGVSPLVLEREYMQGVLIVEKIIQYSEVTHNNNKNKKQDAFSVKKVKTRSCIWAFFKTIKAII*