AlfalfaGEDB | Msa1213520

M. sativa cultivar zhongmu-4 / Msa1213520

KEGG: -
GO: GO:0000070 GO:0000228 GO:0000278 GO:0000280 GO:0000444 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000818 GO:0000819 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006996 GO:0007049 GO:0007059 GO:0008104 GO:0008150 GO:0009987 GO:0016043 GO:0022402 GO:0022607 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0033365 GO:0034501 GO:0034502 GO:0034508 GO:0034613 GO:0034622 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0048285 GO:0051179 GO:0051276 GO:0051382 GO:0051383 GO:0051641 GO:0065003 GO:0065004 GO:0070013 GO:0070727 GO:0070925 GO:0071459 GO:0071824 GO:0071840 GO:0098687 GO:0098813 GO:0140014 GO:1903047
PFAM: PF05859.15

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1213520	sp\|Q2V0Z5\|MIS12_ARATH	41.803	244	124	7	1	239	1	231	2.65e-53	175

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1213520	G7JT53	93.496	246	16	0	1	246	4	249	8.31e-171	480

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0003890	Msa1213520	0.803097	4.100397e-49	-8.615850e-47
Msa0011660	Msa1213520	-0.802088	6.621836e-49	-8.615850e-47
Msa0084380	Msa1213520	0.800437	1.441934e-48	-8.615850e-47
Msa0150840	Msa1213520	0.808355	3.225862e-50	-8.615850e-47
Msa0166560	Msa1213520	0.806607	7.578196e-50	-8.615850e-47
Msa0180500	Msa1213520	0.818688	1.720074e-52	-8.615850e-47
Msa0211150	Msa1213520	0.804625	1.974326e-49	-8.615850e-47
Msa0267370	Msa1213520	0.812067	5.107743e-51	-8.615850e-47
Msa0296820	Msa1213520	0.806083	9.770601e-50	-8.615850e-47
Msa0317970	Msa1213520	0.806762	7.026064e-50	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1213520	MtrunA17_Chr4g0061861	93.496	246	16	0	1	246	4	249	1.60e-174	480
Msa1213520	MtrunA17_Chr4g0062071	92.276	246	16	1	1	246	1	243	8.04e-157	435
Msa1213520	MtrunA17_Chr4g0062041	88.889	36	4	0	201	236	2	37	2.07e-15	68.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1213520	AT5G35520.1	41.803	244	124	7	1	239	1	231	2.69e-54	175

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Find 75 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATACATTACAGGTCACA+GGG	0.652192	8_1:-73669683	Msa1213520:three_prime_UTR
AACCACTCATTTCAAATGTA+TGG	0.432061	8_1:-73669400	Msa1213520:three_prime_UTR
AAGATCCCAACATCTCACAC+AGG	0.719465	8_1:-73671752	Msa1213520:intron
AAGGATTGACAACAATAAAA+TGG	0.285180	8_1:-73670063	Msa1213520:CDS
ACCAACAAGATTGAAGAGAC+CGG	0.579940	8_1:-73670101	Msa1213520:CDS
ACTAGGTTGTGATAAAGCCC+TGG	0.575518	8_1:-73669608	Msa1213520:three_prime_UTR
ACTTCGTTGAAGAAGAGTTG+TGG	0.543092	8_1:+73671947	None:intergenic
AGCATCCACCAAGCTCAATG+CGG	0.721723	8_1:-73671785	Msa1213520:CDS
AGCGAATCTCTACTTTCTAA+AGG	0.484540	8_1:+73669362	None:intergenic
AGCTTCCGCATTGAGCTTGG+TGG	0.510752	8_1:+73671780	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TAATTCTATCATTGTACGGT+TGG	-	chr8_1:73669373-73669392	Msa1213520:three_prime_UTR	30.0%
ATTAGCGAATAACAACAAGT+TGG	+	chr8_1:73669960-73669979	None:intergenic	30.0%
CATCAAAATCTATGCTATTG+CGG	-	chr8_1:73670025-73670044	Msa1213520:intron	30.0%
AAGAGATTAAAGGAAATGCA+CGG	-	chr8_1:73670077-73670096	Msa1213520:CDS	30.0%
ATTGAGGAAAAAACTAGTTG+AGG	-	chr8_1:73670419-73670438	Msa1213520:intron	30.0%
CAAACAAACAGGTATCAAAA+TGG	+	chr8_1:73670769-73670788	None:intergenic	30.0%
TTGATAGGAGGACTATATTT+AGG	-	chr8_1:73671036-73671055	Msa1213520:CDS	30.0%
TGATAGGAGGACTATATTTA+GGG	-	chr8_1:73671037-73671056	Msa1213520:CDS	30.0%
TCTGCTATATATGCTTCTAA+AGG	-	chr8_1:73671399-73671418	Msa1213520:intron	30.0%
CTTCAAATTTCGTATTAGAG+AGG	+	chr8_1:73671512-73671531	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	73669357	73672099	73669357	ID=Msa1213520;Name=Msa1213520
chr8_1	mRNA	73669357	73672099	73669357	ID=Msa1213520-mRNA-1;Parent=Msa1213520;Name=Msa1213520-mRNA-1;_AED=0.08;_eAED=0.08;_QI=70\|1\|1\|1\|1\|1\|7\|530\|246
chr8_1	exon	73669357	73669948	73669357	ID=Msa1213520-mRNA-1:exon:25128;Parent=Msa1213520-mRNA-1
chr8_1	exon	73670036	73670173	73670036	ID=Msa1213520-mRNA-1:exon:25127;Parent=Msa1213520-mRNA-1
chr8_1	exon	73670776	73670905	73670776	ID=Msa1213520-mRNA-1:exon:25126;Parent=Msa1213520-mRNA-1
chr8_1	exon	73671016	73671108	73671016	ID=Msa1213520-mRNA-1:exon:25125;Parent=Msa1213520-mRNA-1
chr8_1	exon	73671512	73671637	73671512	ID=Msa1213520-mRNA-1:exon:25124;Parent=Msa1213520-mRNA-1
chr8_1	exon	73671753	73671810	73671753	ID=Msa1213520-mRNA-1:exon:25123;Parent=Msa1213520-mRNA-1
chr8_1	exon	73671896	73672099	73671896	ID=Msa1213520-mRNA-1:exon:25122;Parent=Msa1213520-mRNA-1
chr8_1	five_prime_UTR	73672030	73672099	73672030	ID=Msa1213520-mRNA-1:five_prime_utr;Parent=Msa1213520-mRNA-1
chr8_1	CDS	73671896	73672029	73671896	ID=Msa1213520-mRNA-1:cds;Parent=Msa1213520-mRNA-1
chr8_1	CDS	73671753	73671810	73671753	ID=Msa1213520-mRNA-1:cds;Parent=Msa1213520-mRNA-1
chr8_1	CDS	73671512	73671637	73671512	ID=Msa1213520-mRNA-1:cds;Parent=Msa1213520-mRNA-1
chr8_1	CDS	73671016	73671108	73671016	ID=Msa1213520-mRNA-1:cds;Parent=Msa1213520-mRNA-1
chr8_1	CDS	73670776	73670905	73670776	ID=Msa1213520-mRNA-1:cds;Parent=Msa1213520-mRNA-1
chr8_1	CDS	73670036	73670173	73670036	ID=Msa1213520-mRNA-1:cds;Parent=Msa1213520-mRNA-1
chr8_1	CDS	73669887	73669948	73669887	ID=Msa1213520-mRNA-1:cds;Parent=Msa1213520-mRNA-1
chr8_1	three_prime_UTR	73669357	73669886	73669357	ID=Msa1213520-mRNA-1:three_prime_utr;Parent=Msa1213520-mRNA-1

Gene Sequence

>Msa1213520

ATGGAAGGAAGCATGAGCAGAGAGAGCGACGCTGTTTTCGAATCGGTGATGAATCTGAATCCACAACTCTTCTTCAACGAAGTTCTCAACACTGTTGATGATTTCGTCCTCGATACTTTCGATTTCTACTTCCAGGAAGCATCCACCAAGCTCAATGCGGAAGCTACTCAAAGATCCCAACATCTCACACAGGGTGTTGATTGCATTCGTCAAAAGGTTCAATCTGTTTTGGATCAAAAGCTCGCTGTTTGGGAAAAATACTGCCTTTATCATTGTTTTTCTCTTCCCGAAGGTTTTCAATTGCCAAACACTCTCAATGGTGAAACAAGTGGGAATGTCATTGATCCTGGTTCTACTTCCAATCTGGAATTAGATGCTCAGTTAGAATCATTGAGGAAAAAACTAGTTGAGGTGGGGAAAGAGTCTGAAATGCTGAATCAAGAAATTCATGCATTAGAGAGTCAGTCTTCTCACAATGCTCGGTATATCAACGAGGCAGTACAATTATTTGAGCAAAACTCTTACGCTGAGTTGTTTCAGGAGATTATGGCAACCGCCTCAGAACTTCGACTCAAGATAGGAAAGCTAAACACCAACAAGATTGAAGAGACCGGAAAAATGAAAGCAAAAAGGATTGACAACAATAAAATGGACATTTCTGCTATATATGCTTCTAAAGGCCTCTCTAATACGAAATTTGAAGATCTTCAAGAATTTGTAACTCTTATGAAGAGTACTTGA

Protein sequence

>Msa1213520

MEGSMSRESDAVFESVMNLNPQLFFNEVLNTVDDFVLDTFDFYFQEASTKLNAEATQRSQHLTQGVDCIRQKVQSVLDQKLAVWEKYCLYHCFSLPEGFQLPNTLNGETSGNVIDPGSTSNLELDAQLESLRKKLVEVGKESEMLNQEIHALESQSSHNARYINEAVQLFEQNSYAELFQEIMATASELRLKIGKLNTNKIEETGKMKAKRIDNNKMDISAIYASKGLSNTKFEDLQEFVTLMKST*